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At least 343 records · Page 19
All sourcesNASA NTRSDOE OSTI
DOE OSTI2025

Arabidopsis GALACTURONOSYLTRANSFERASE (GAUT) 1 synthesizes a homogalacturonan tightly bound to the cell wall and required for cell expansion

Arabidopsis GALACTURONOSYLTRANSFERASE1 (GAUT1) synthesizes homogalacturonan (HG), the most abundant pectin in growing plant cells. GAUT1 has the greatest in vitro enzyme activity of the six confirmed Arabidopsis HG biosynthetic GAUTs, but its biological activity remains elusive. Here we show that Arabidopsis GAUT1 homozygous mutants have a severe dwarfed seedling phenotype, survive several weeks as 2 to 3 mm seedlings, and have severely reduced shoot and root growth and hypocotyl epidermal, cortex and endodermal cell size. gaut1-1 pollen tubes are shorter than WT with increased bursting. Complementation of homozygous gaut1-1 with GAUT1 coding sequence driven by the GAUT1 promoter restored WT-like growth. The extreme dwarf phenotype of homozygous gaut1-1 seedlings precluded their use for detailed cell wall analysis, thus suspensions cultures were produced from callus generated from mutant and WT seedlings. Homozygous gaut1-1 suspension cells were smaller than WT with ∼30% reduced wall GalA content compared to WT. Sequential extraction of the walls with increasingly harsh solvents and sugar composition analysis revealed reduced GalA content in only the 4M KOH post-chlorite fraction, indicating that GAUT1-synthesized HG was held tightly in the wall by direct or indirect hydrogen bonding and/or oxidation-sensitive linkages. Treatment of wall fractions with endopolygalacturonase to hydrolyze HG and gel electrophoretic separation of hydrolysates exposed an HG-associated doublet band markedly downregulated in the homozygous gaut1-1 4M KOH post-chlorite fraction and to a lesser extent in 4M KOH and sodium chlorite fractions. NMR analysis identified the band as rhamnogalacturonan (RG)-II. Super resolution microscopy using anti-HG antibodies showed that, compared to WT, the homozygous gaut1-1 hypocotyl epidermal and callus cells had reduced content and length of HG nanofilaments, HG fibers associated with cell expansion in Arabidopsis. The results demonstrate that GAUT1-synthesized HG resides in a tightly-cell-wall-bound, RG-II-containing polymer required for HG nanofilament formation and seedling cell expansion.

Atmodjo↗
NASA NTRS1975

Low-speed upwash interference on a transport model in a rectangular slotted-wall wind tunnel

A study was made of the upwash interference caused by the wind tunnel walls at a Mach number of 0.20. The wind tunnel has slotted horizontal walls and solid vertical walls and the wind tunnel model is a wing-fuselage combination typical of a short take-off and landing (STOL) transport. Measurements were made of the model forces and angle of attack. The experimental results are compared to theoretical solutions for the upwash interference. This comparison enabled an indirect determination of one of the constants in the slotted wall boundary condition. The magnitude of the experimental upwash interference is also compared to the accuracy of the data. This comparison indicates that it is difficult to make definite conclusions based on the experimental data. Suggestions are made for future research which could provide a practical means of accurately determining the wall-interference velocities in wind tunnels with rigid slotted walls.

Mann, M. J.↗
NASA NTRS1978

A parametric experimental study of the interference effects and the boundary-condition coefficient of slotted wind-tunnel walls

An experimental study of slotted upper and lower walls in a two-dimensional transonic wind tunnel with solid sidewalls is reported. Results are presented for several slot spacings and slot openness ratios. The experimental data are pressure measurements which were made on an airfoil model and on a sidewall near one of the slotted walls. The slotted-wall boundary-condition coefficient, which relates the pressure and streamline curvature near the wall, is determined from the wall pressure measurements. The measured wall-induced interference is correlated with the experimental values for the boundary-condition coefficient. This correlation is compared with theory.

Everhart, J. L.↗
NASA NTRS1978

A parametric experimental study of the slotted-wall boundary condition

An experimental study of slotted upper and lower walls in a two dimensional transonic wind tunnel with solid sidewalls is reported. Results are presented for several slot spacings and slot openness ratios. The experimental data were pressure measurements which were made on an airfoil model and on a sidewall near one of the slotted walls. The slotted-wall boundary condition coefficient, which related the pressure and streamline curvature near the wall, was determined from the wall pressure measurements. The measured wall-induced interference was correlated with the experimental values for the boundary condition coefficient. This correlation was compared with theory.

Everhart, J. L.↗
NASA NTRS1981

Three-dimensional structures and turbulence closure of the wake developing in a wall shear layer

The turbulent wake interacting with the rotating wall shear layer is investigated analytically and numerically. The turbulent wakes of the rotating blades in a compressor which are interacting with the rotating hub-wall boundary layer are analyzed. A modified version of the closure model of the pressure-strain correlation term in the Reynolds stress transport equation is developed to predict the effect of rotation, which is appreciable for the present flow because the thick hub-wall boundary layer is interacting with the rotor wake. It is noted that the Poisson type equation for the pressure-strain correlation has an extra rotation term when the entire flow field is rotating. This extra rotation term is modeled to accommodate the effect of rotation. In addition, the standard correction for the wall effect is incorporated for the utilized Reynolds stress closure model. The rotation-modified Reynolds stress closure model is used to predict the present flow, and the predictions are compared with the experimental data. The experimental data reveal that the characteristics of the three-dimensional turbulent wake interacting with the wall shear layer are considerably altered by the effects of the wall and the rotation. These features are predicted with good accuracy by the turbulence closure model developed.

Hah, C.↗
NASA NTRS1981

Error in Dasibi flight measurements of atmospheric ozone due to instrument wall-loss

Theory suggests that in laminar flow the percent loss of a trace constituent to the walls of a measuring instrument varies as P to the -2/3, where P is the total gas pressure. Preliminary laboratory ozone wall-loss measurements confirm this P to the -2/3 dependence. Accurate assessment of wall-loss is thus of particular importance for those balloon-borne instruments utilizing laminar flow at ambient pressure, since the ambient pressure decreases by a factor of 350 during ascent to 40 km. Measurements and extrapolations made for a Dasibi ozone monitor modified for balloon flight indicate that the wall-loss error at 40 km was between 6 and 30 percent and that the wall-loss error in the derived total ozone column-content for the region from the surface to 40 km altitude was between 2 and 10 percent. At 1000 mb, turbulence caused an order of magnitude increase in the Dasibi wall-loss.

Ainsworth, J. E.↗
NASA NTRS1982

Adaptive-wall wind-tunnel research at Ames Research Center

Adaptive-wall wind-tunnel research is summarized. This research includes small-scale two- and three-dimensional wind-tunnel experiments and numerical experiments with a three-dimensional adaptive-wall simulator. Airflow through the test-section walls is controlled by adjusting the pressures in segmented plenums. Interference free conditions are successfully attained in subsonic and transonic flows. An adaptive wall test section is constructed for the transonic wind tunnel. Wall interference was reduced in the three dimensional experiment at several angles of attack at Mach 0.60. A wing on wall configuration was modeled in the numerical experiments. These flow simulations showed that free air conditions can be approximated by adjusting boundary conditions at only the floor and ceiling of the test section. No sidewall control was necessary.

Schairer, E. T.↗
NASA NTRS1984

Survey of ONERA activities on adaptive-wall applications and computation of residual corrections

The research undertaken concerning the computation and/or reduction of wall interference follows two main axes: improvement of wall correction determinations, and use of adaptive flexible walls. The use of wall-measured data to compute interference effects is reliable when the model representation is assessed by signatures with known boundary conditions. When the computed interferences are not easily applicable to correcting the results (especially for gradients in two-dimensional cases), the flexible adaptive walls in operation in T2 are an efficient and assessed means of reducing the boundary effects to a negligible level, if the direction and speed of the flow are accurately measured on the boundary. The extension of the use of adaptive walls to three-dimensional cases may be attempted since the residual corrections are assumed to be small and are computable.

Chevallier, J. P.↗
NASA NTRS1984

Investigations of flow field perturbations induced on slotted transonic-tunnel walls

The free-stream interference caused by the flow through the slotted walls of the test sections of transonic wind tunnels has continuously a problem in transonic tunnel testing. The adaptive-wall transonic tunnel is designed to actively control the near-wall boundary conditions by sucking or blowing through the wall. In order to make the adaptive-wall concept work, parameters for computational boundary conditions must be known. These parameters must be measured with sufficient accuracy to allow numerical convergence of the flow field computations and must be measured in an inviscid region away from the model that is placed inside the wind tunnel. The near-wall flow field was mapped in detail using a five-port cone probe that was traversed in a plane transverse to the free-stream flow. The initial experiments were made using a single slot and recent measurements used multiple slots, all with the tunnel empty. The projection of the flow field velocity vectors on the transverse plane revealed the presence of a vortex-like flow with vorticity in the free stream. The current research involves the measurement of the flow field above a multislotted system with segmented plenums behind it, in which the flow is controlled through several plenums simultaneously. This system would be used to control a three-dimensional flow field.

Wu, J. M.↗
NASA NTRS1986

Wall layer models for the calculation of velocity and heat transfer in turbulent boundary layers

In the computation of turbulent boundary-layer flows and internal flows, a substantial amount of mesh points and computational effort is required to adequately resolve the intense temperature and velocity profile variations in the near wall region. In this study, analytical profile approximations are obtained for the mean velocity and temperature distribution in the wall layer; these profiles are based upon consideration of the observed coherent structure of the time-dependent wall-layer flow. The profile approximations are relatively simple analytical formulae which satisfy all the required compatibility conditions at the wall and the logarithmic behavior at the edge of the wall layer. The Reynolds analogy for heat transfer is not used in the present theory. A general method for utilizing the present wall-layer profile models in a prediction method is indicated.

Walker, J. D. A.↗
NASA NTRS1986

Applications of a new wall function to turbulent flow computations

A new wall function approach is developed based on a wall law suitable for incompressible turbulent boundary layers under strong adverse pressure gradients. This wall law was derived from a one-dimensional analysis of the turbulent kinetic energy equation with gradient diffusion concept employed in modeling the near-wall shear stress gradient. Numerical testing cases for the present wall functions include turbulent separating flows around an airfoil and turbulent recirculating flows in several confined regions. Improvements on the predictions using the present wall functions are illustrated. For cases of internal recirculating flows, one modification factor for improving the performance of the k-epsilon turbulence model in the flow recirculation regions is also included.

Chen, Y. S.↗
NASA NTRS1985

The effects of compliant walls on transition and turbulence

Two theoretical approaches to the phenomenon of drag reduction by a compliant wall are proposed. One is to study the limit cycle oscillations of the Navier-Stokes equations for a boundary layer flow over a compliant wall or a Poiseuille flow in a channel with a compliant wall. The other is a complementary study of a simpler, sequential model, which partially decouples the fluid and wall motions. In this approach, a 'Total Model' is constructed from suitable component models. While the first study is more rigorously based, it will be initially limited, by computational constraints, to relatively low Reynolds numbers. The second, more approximate approach, will allow consideration of a fully developed turbulent flow. The Total Model will predict the compliant wall motion accurately, but will not per se be sufficient to predict the skin friction drag. However, once the wall motion is known, the skin friction drag and the pressure drag can be computed separately, using one of the existing models.

Dowell, E. H.↗
NASA NTRS1986

A theory for turbulent curved wall jets

A simple theoretical model is proposed to describe the flow of a turbulent wall jet along a curved surface into a quiescent atmosphere. An integral method is used to solve the momentum equation and identifies three contributions to the spreading rate of the wall jet: (1) turbulent diffusion in the wall jet; (2) wall curvature; and (3) rate of change of wall curvature. Closed from approximate solutions are found for the case of a plane wall, a circular cylinder, and a logarithmic spiral surface. Comparison with experimental data for these three cases is made showing good agreement.

Roberts, Leonard↗
NASA NTRS1981

Flexiwall 3 SO: A second order predictive strategy for rapid wall adjustment in two-dimensional compressible flow

An improvement is presented for the 2-D strategies for adjustment of the flexible top and bottom walls of an Adaptive (Wind Tunnel) Wall Test Section (AWTS). This adjustment is part of the wall adaptation process to eliminate top and bottom wall interference at the source. The improvements to account for second order effects are described in mathematical detail. It is intended that these improvements should further minimize the necessary iterations in the wall adaptation process. An associated computer program written in BASIC is presented and several test cases run with this program are discussed. The strategy performs well for a theoretical test case but when applied to experimental AWTS data some discrepancies in the adapted wall shapes are found.

Goodyer, M. J.↗
NASA NTRS1988

Empty test section streamlining of the transonic self-streamlining wind tunnel fitted with new walls

The original flexible top and bottom walls of the Transonic Self-Streamlining Wind Tunnel (TSWT), at the University of Southampton, have been replaced with new walls featuring a larger number of static pressure tappings and detailed mechanical improvements. This report describes the streamling method, results, and conclusions of a series of tests aimed at defining sets of aerodynamically straight wall contours for the new flexible walls. This procedure is a necessary prelude to model testing. The quality of data obtained compares favorably with the aerodynamically straight data obtained with the old walls. No operational difficulties were experienced with the new walls.

Lewis, M. C.↗
NASA NTRS1988

Highlights of experience with a flexible walled test section in the NASA Langley 0.3-meter transonic cryogenic tunnel

The unique combination of adaptive wall technology with a continuous flow cryogenic wind tunnel is described. This powerful combination allows wind tunnel users to carry out two-dimensional (2-D) tests at flight Reynolds numbers with wall interferences essentially eliminated. Validation testing was conducted to support this claim using well tested symmetrical and cambered airfoils at transonic speeds and high Reynolds numbers. The test section hardware has four solid walls, with the floor and ceiling flexible. The method of adapting/shaping the floor and ceiling to eliminate top and bottom wall interference at its source is outlined. Data comparisons for different size models tested and others in several sophisticated 2-D wind tunnels are made. In addition, the effects of Reynolds number, testing at high lift with associated large flexible wall movements, the uniqueness of the adapted wall shapes, and the effects of sidewall boundary layer control are examined. The 0.3-m TCT is now the most advanced 2-D research facility anywhere.

Wolf, Stephen W. D.↗
NASA NTRS1988

Highlights of experience with a flexible walled test section in the NASA Langley 0.3-meter transonic cryogenic tunnel

The unique combination of adaptive wall technology with a contonuous flow cryogenic wind tunnel is described. This powerful combination allows wind tunnel users to carry out 2-D tests at flight Reynolds numbers with wall interference essentially eliminated. Validation testing was conducted to support this claim using well tested symmetrical and cambered airfoils at transonic speeds and high Reynolds numbers. The test section hardware has four solid walls, with the floor and ceiling flexible. The method of adapting/shaping the floor and ceiling to eliminate top and bottom wall interference at its source is outlined. Data comparisons for different size models tested and others in several sophisticated 2-D wind tunnels are made. In addition, the effects of Reynolds number, testing at high lift with associated large flexible wall movements, the uniqueness of the adapted wall shapes, and the effects of sidewall boundary layer control are examined. The 0.3-m TCT is now the most advanced 2-D research facility anywhere.

Wolf, Stephen W. D.↗