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At least 343 records · Page 19

Genomics, Exometabolomics, and Metabolic Probing Reveal Conserved Proteolytic Metabolism of Thermoflexus hugenholtzii and Three Candidate Species From China and Japan

Thermoflexus hugenholtzii JAD2 T , the only cultured representative of the Chloroflexota order Thermoflexales, is abundant in Great Boiling Spring (GBS), NV, United States, and close relatives inhabit geothermal systems globally. However, no defined medium exists for T. hugenholtzii JAD2 T and no single carbon source is known to support its growth, leaving key knowledge gaps in its metabolism and nutritional needs. Here, we report comparative genomic analysis of the draft genome of T. hugenholtzii JAD2 T and eight closely related metagenome-assembled genomes (MAGs) from geothermal sites in China, Japan, and the United States, representing “Candidatus Thermoflexus japonica,” “Candidatus Thermoflexus tengchongensis,” and “Candidatus Thermoflexus sinensis.” Genomics was integrated with targeted exometabolomics and 13 C metabolic probing of T. hugenholtzii. The Thermoflexus genomes each code for complete central carbon metabolic pathways and an unusually high abundance and diversity of peptidases, particularly Metallo- and Serine peptidase families, along with ABC transporters for peptides and some amino acids. The T. hugenholtzii JAD2 T exometabolome provided evidence of extracellular proteolytic activity based on the accumulation of free amino acids. However, several neutral and polar amino acids appear not to be utilized, based on their accumulation in the medium and the lack of annotated transporters. Adenine and adenosine were scavenged, and thymine and nicotinic acid were released, suggesting interdependency with other organisms in situ. Metabolic probing of T. hugenholtzii JAD2 T using 13 C-labeled compounds provided evidence of oxidation of glucose, pyruvate, cysteine, and citrate, and functioning glycolytic, tricarboxylic acid (TCA), and oxidative pentose-phosphate pathways (PPPs). However, differential use of position-specific 13 C-labeled compounds showed that glycolysis and the TCA cycle were uncoupled. Thus, despite the high abundance of Thermoflexus in sediments of some geothermal systems, they appear to be highly focused on chemoorganotrophy, particularly protein degradation, and may interact extensively with other microorganisms in situ.

59 BASIC BIOLOGICAL SCIENCES↗

First restults from the Faint Infrared Grism Survey (FIGS): first simultaneous detection of Lyα emission and Lyman break from a galaxy at z = 7.51

Galaxies at high redshifts are a valuable tool for studying cosmic dawn, therefore it is crucial to reliably identify these galaxies. Here, we present an unambiguous and first simultaneous detection of both the Lyα emission and the Lyman break from a z=7.512 ± 0.004 galaxy, observed in the Faint Infrared Grism Survey (FIGS). These spectra, taken with the G102 grism on the Hubble Space Telescope (HST), show a significant emission line detection (6σ) in two observational position angles (PAs), with Lyα line flux of 1.06±0.19×10{sup −17} erg s{sup −1} cm{sup −2}. The line flux is nearly a factor of four higher than that in the archival MOSFIRE spectroscopic observations. This is consistent with other recent observations, implying that ground-based near-infrared spectroscopy underestimates the total emission line fluxes, and if confirmed, can have strong implications for reionization studies that are based on ground-based Lyα measurements. A 4σ detection of the NV line in one PA also suggests a weak active galactic nucleus (AGN), and if confirmed, would make this source the highest-redshift AGN yet found. These observations from HST thus clearly demonstrate the sensitivity of the FIGS survey, and the capability of grism spectroscopy for studying the epoch of reionization.

79 ASTRONOMY AND ASTROPHYSICS↗

Entangling remote superconducting qubits via transducer-generated multi-time-bin states

Recent studies have shown long-distance entanglement using NV centers, atoms, and quantum dots with single-photon time-bin encoding. We propose a method to entangle remote superconducting qubits via microwave-optical transduction using multi-time-bin states. By adapting conventional entanglement swapping techniques, fidelity improves from 0.75 to $0.98$ in transduction systems, and 0.66 to 0.89 in noisy channels. The protocol mitigates thermal noise without relying on purification and offers a practical path toward scalable, heterogeneous quantum systems.

Wu, Jing [Fermilab]↗

Field Validation of a Grid-Interactive Efficient Building Software Solution

The U.S. General Services Administration's (GSA's) Green Proving Ground (GPG) program, in partnership with the National Laboratory of the Rockies (NLR), completed a field study of a Grid-Interactive Efficient Buildings (GEB) software solution. The study focused on a single testbed facility to test the GEB functionality of the software solution, along with other features. The testbed facility - a courthouse - is a common building type in GSA's vast building portfolio, offering potentially impactful findings on a scalable level. The study evaluated Prescriptive Data's technology, Nantum OS, a connected building operating system ("GEB Solution") which aggregates multiple sources of previously siloed building data and combines that data with external sources, such as weather information or utility signals, into a single integrated platform. A GEB Solution is a type of Energy Management Information System (EMIS). EMIS is defined as a system of devices, data services, and software applications that communicates with any building system or third-party data source to aggregate and transform data into new capabilities to aid in the optimization of energy use at the building, campus, or agency level. This specific GEB Solution is an EMIS with ASO, automated system optimization, offering supervisory control of certain aspects of the Building Automation System (BAS). Multiple features were evaluated including, but not limited to, Continuous Demand Management to avoid setting new monthly kilowatt (kW) peaks, energy efficiency for reduction of kilowatt hours (kWh) and natural gas consumption, and automated demand response (ADR) for purposes of lowering demand during a utility called Demand Response (DR) event. The testbed facility was the Foley Federal Building and US Courthouse ("Foley Federal Building") located in Las Vegas, NV. This is a 209,496 sq. ft. building constructed in the 1960s with major renovations in 2004. The facility was a good candidate due to the large prevalence of office and courthouse spaces in the GSA portfolio of buildings. It also has many features which allow integration into and control of the building and a strong facilities team to assist with the study. Quantitative and qualitative performance objectives were developed using GSA's GPG GEB project template along with input from the vendor and building facility staff; these are outlined in Table 1. The quantitative performance objectives focused on continuous demand management, energy efficiency, and automated demand response. The qualitative performance objectives focused on the ease of installation and commissioning as well as the operability of the GEB solution. Other performance metrics that are reported on include carbon reduction, cost effectiveness, and occupant acceptance.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗

Electrochemical approach for passivating steel and other metals and for the simultaneous production of a biocide to render water potable

Potentiostatic polarization curves indicated that the cathodic reactions in deaerated KI-I2 water solutions were due to iodine reduction and hydrogen evolution. In the presence of oxygen an additional reduction wave appeared. Anodic polarization curves revealed that iodine could be produced in the region of potential from +600 to +1000 nv vs. SCE.

Source record↗

Line strength variations in beta Cephei

The line strength variations of the resonance line of C IV (1550A, 2s 2S - 2P) observed by OAO-2 were confirmed by IUE observations. In addition, the NV resonance line (1204A, 2s 2S - 2P), the Si III line (1206A, 3p 1P-1D, multiplet 11) and the Si IV resonance line (1395A, 3s 2S - 2P) all vary in line strength essentially in phase with the C IV variation. The (preliminary) period of the variation is 6.02/12.04 days.

Fischel, D.↗

On the nature of the nova-like variable CD-42 deg 14462

Low dispersion long and short wavelength IUE spectra of the nova like system CD-42 deg 14462 were obtained on August 24 U.T. The short wave spectrum exhibits absorption features due to C III (lambda 1175), Lalpha 1216), NV (lambda1240), HeII (lambda 1640), SiIV (lambda1394), NIV (lambda1875) with CIV (lambda1550) as a P Cygni feature with blue shifted absorption suggesting the presence of material leaving the system. Possible interpretations of this object are discussed.

Guinan, E. F.↗

Orbital phase dependent IUE spectra of the nova like binary II Arietis

Nine low dispersion IUE spectra of the nova like binary TT Ari over its 3h17m orbital period were obtained. Four short wave spectra and five long wave spectra exhibit marked changes in line strength and continuum shape with orbital phase. The short wave spectra show the presence in absorption of C III, Lyman alpha, SiIII, NV, SiIV, CIV, HeII, AlIII, and NIV. The CIV shows a P Cygni profile on two of the spectra. Implications of these spectra for the nature of nova like variables are discussed.

Guinan, E. F.↗

The high velocity symbiotic star AG Draconis after its 1980 outburst

High and low resolution spectra of AG Dra taken in 1981 are analyzed. The UV spectrum of AG Dra is characterized by prominent high ionization emission lines superimposed on a strong continuum. At high resolution, several intense absorption lines of interstellar origin are seen, in spite of the low interstellar extinction. A similar situation is displayed by the high galactic latitude sd0 stars. The radial velocity difference between the emission lines and the i.s. lines is about -105 Km/sec in agreement with the optical observations. The He II 1640 A line appears much stronger than in other symbiotic stars and suggests the presence of a hot source which is variable according to the activity of the star. The line also exhibits broad emission wings which could be formed in a rotating disk. The NV resonance doublet displays a P Cygni profile and is probably formed in a warm wind. Two components in the UV continuum are identified: a steep component dominating the far UV probably associated with the hot source, and a flatter continuum in the near UV which cannot be accounted for by f-f and f-b emission alone, but which is probably emitted by an optically thick region or disk.

Viotti, R.↗

Observations of mass accretion in binary stars

Results from high resolution observations of eight close binary stars (TX UMa, U CrB, CX Dra, TT Hya, AU Mon, KX And, HR 2142, and phi Per) are presented. Variable absorption lines, indicative of mass flow, are observed in all systems expect phi Per. Emission lines are seen in KX And and phi Per. Variable high ionization features (NV, SiIV, and CIV) are seen in TX UMa, UCrB, CX Dra, and AU Mon. The observations are modeled using the calculations of Lubow and Shu.

Polidan, R. S.↗

Optically-thick, highly-variable plasma in interacting binaries

The interacting binaries HD 207739 and R Arae were observed by IUE. The ultraviolet spectrum of HD 207739 undergoes complex variations in both the lines and the continuum. Considerable circumstellar matter is present; R Arae (B9p + ?) exhibits SiIV, CIV and even NV absorption features. Both the lines and continuum are variable. An unusual secondary eclipse which is deeper in the ultraviolet than in the visible is present near phase 0.6. The CII line has a P cygni profile with emission present at all observed phases. These systems are probably in a relatively short-lived phase of evolution which is not often observed.

Kondo, Y.↗

A search for quasars in the Virgo cluster region

Using objective-prism plates taken with the 44-arcmin objective prism mounted on the UK Schmidt telescope, 53 emission-line quasar candidates and 29 ultraviolet-excess objects (possible low-redshift quasars) have been found in a 5 x 5-degree field centered on 12 h 27 m, + 13 deg 30 min (1950) in the Virgo cluster of galaxies. Eighteen of these 82 candidates were observed using the double spectrograph on the Palomar 5-meter telescope; 13 of the observed objects proved to be quasars. The broad-absorption-line QSO Q1232 + 134 is the first example of the class to show broad low-ionization absorption lines (such as Mg II 2798 A) in addition to the usual high-excitation lines such as Nv 1240 A. Although no conclusive evidence for quasar-galaxy associations is found in this field, there do exist nonuniformities in the distribution of the quasar candidates which may merit further investigation. These objects will provide a useful network of probes for absorbing material in the Virgo cluster. The lines-of-sight to two of the confirmed quasars pass very close to NGC galaxies; the respective projected QSO-galaxy separations are only 4 and 11 kpc at the assumed distance of the Virgo cluster.

He, X.-T.↗

Influence of rock-soil spectral variation on the assessment of green biomass

A comparison of how n-spaced and ratio-based vegetation indices respond to rock and soil spectral variation is made, using a set of ground-based reflectance spectra and airborne Thematic Mapper imagery of the Virginia Range, NV. The influence of variations in rock-soil brightness on ratio-based vegetation indices is also discussed. It is shown that of all the vegetation indices tested, the perperdicular vegetation index is the most appropriate for use in multispectral imagery of arid and semiarid regions where there is a wide variation in substrate characteristics.

Elvidge, C. D.↗

Field Utilization and Analysis of AIS 128-channel Imagery Using Microcomputers: Application to Yerington, Nevada Field Area

Geologists in exploration need to be able to determine the mineral composition of a given outcrop, and then proceed to another in order to carry out the process of geologic mapping. Since April 1984 researchers have been developing a portable microcomputer-based imaging system (with a grey-scale of 16 shades of amber), which were demonstrated during the November 1984 GSA field trip in the field at Yerington, NV. A color-version of the same technology was recently demonstrated. The portable computer selected is a COLBY 10-Megabyte, hard disk-equipped repackaged-IBM/XT, which operates on either 110/220 VAC or on 12VDC from the cigarette lighter in a field vehicle. A COMPAQ PLUS or an IBM Portable will also work on modified software. The underlying concept is that the atmospheric transmission and surface albedo/slope terms are multiplicative, relating the spectral irradiance to the spectral color of the surface materials. Thus, the spectral color of a pixel remains after averaged log-albedo and log-irradiance have been estimated. All these steps can be carried out on the COLBY microcomputer, using 80 image lines of the 128-channel, 12-bit imagery. Results are shown for such an 80-line segment, showing the identification of an O-H bearing mineral group (of slightly varying specific characters) on the flight line.

Lyon, R. J. P.↗

Planetary ecology; Proceedings of the Sixth International Symposium on Environmental Biogeochemistry, Santa Fe, NM, October 10-14, 1983

Topics presented include biological evolution and planetary chemistry; C-1 compounds; transport, deposition, and weathering; sulfur transformations; ground water; transformation processes for nitrogen oxides; and soils. Papers are presented on immunological studies on the organic matrix of recent and fossil invertebrate shells; biogenic gases in sediments deposited since Miocene times on the Walvis Ridge, South Atlantic Ocean; aspects of the biogeochemistry of Big Soda Lake, NV; mesophilic manganese-oxidizing bacteria from hydrothermal discharge areas at 21 deg North on the East Pacific Rise; and autotrophic growth and iron oxidation and inhibition kinetics of Leptospirillum ferrooxidans. Consideration is also given to thermophilic archaebacteria occurring in submarine hydrothermal areas; fate of sulfate in a soft-water, acidic lake; geochemical conditions in the ground water environment; microbial transformations as sources and sinks for nitrogen oxides; and the biogeochemistry of soil phosphorus.

Caldwell, D. E.↗

Non-equilibrium ionization around clouds evaporating in the interstellar medium

It is of prime importance for global models of the interstellar medium to know whether dense clouds do or do not evaporate in the hot coronal gas. The rate of mass exchanges between phases depends very much on that. McKee and Ostriker's model, for instance, assumes that evaporation is important enough to control the expansion of supernova remnants, and that mass loss obeys the law derived by Cowie and McKee. In fact, the geometry of the magnetic field is nearly unknown, and it might totally inhibit evaporation, if the clouds are not regularly connected to the hot gas. Up to now, the only test of the theory is the U.V. observation (by the Copernicus and IUE satellites) of absorption lines of ions such as OVI or NV, that exist at temperatures of a few 100,000 K typical of transition layers around evaporating clouds. Other means of testing the theory are discussed.

Ballet, J.↗

Viscous Transonic Airfoil Workshop compendium of results

Results from the Viscous Transonic Airfoil Workshop held at the AIAA 25th Aerospace Sciences Meeting at Reno, NV in January 1987, are compared with each other and with experimental data. Test cases used in this workshop include attached and separated transonic flows for three different airfoils: the NACA 0012 airfoil, the RAE 2822 airfoil, and the Jones airfoil. A total of 23 sets of numerical results from 15 different author groups are included. The numerical methods used vary widely and include: 16 Navier-Stokes methods, 2 Euler/boundary-layer methods, and 5 full-potential/boundary-layer methods. The results indicate a high degree of sophistication among the numerical methods with generally good agreement between the various computed and experimental results for attached or moderately-separated cases. The agreement for cases with larger separation is only fair and suggests additional work is required in this area.

Holst, Terry L.↗

Discrimination of hydrothermal alteration mineral assemblages at Virginia City, Nevada, using the airborne imaging spectrometer

The purpose of this study is to use airborne imaging spectrometer data to discriminate hydrothermal alteration mineral assemblages associated with silver and gold mineralization at Virginia City, NV. The data is corrected for vertical striping and sample gradients, and converted to flat-field logarithmic residuals. Log residual spectra from areas known to be altered are compared to field spectra for kaolinitic, illitic, sericitic, and propylitic alteration types. The areal distributions of these alteration types are estimated using a spectral matching technique. Both visual examination of spectra and the matching techniques are effective in distinguishing kaolinitic, illitic, and propylitic alteration types from each other. However, illitic and sericitic alteration cannot be separated using these techniques because the spectra of illite and sericite are very similar. A principal components analysis of 14 channels in the 2.14-2.38 micron wavelength region is also successful in discriminating and mapping illitic, kaolinitic, and propylitic alteration types.

Hutsinpiller, Amy↗