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At least 343 records · Page 19
All sourcesNASA NTRSDOE OSTI
DOE OSTI2025

Investigating Bacterial-Fungal Interactions using Fungal Highway Columns in Diverse Environments and Substrates

Bacterial-fungal interactions (BFIs) play an integral role in shaping microbial community composition, biogeochemical functions, spatial dynamics, and microbial dispersal. Mycelial networks created by filamentous fungi or other filamentous microorganisms (e.g., Oomycetes) act as 'fungal highways' that can be utilized by bacteria for transport throughout heterogeneous environments, greatly facilitating their mobility and granting them access to regions that may be challenging or impossible to reach on their own (e.g., due to air pockets within the soil). Several devices and experimental protocols have been created to study these fungal highways, including fungal highway columns. The fungal highway column designed by our group can be used for a variety of in situ or in vitro applications, as well as with diverse environmental and host-associated sample types. Herein, we describe the methods for performing experiments with these columns, including designing, printing, sterilizing, and preparing the devices. The options for analyzing data obtained from the use of these devices are also discussed here, and troubleshooting advice regarding potential pitfalls associated with experiments using fungal highway columns is offered. These devices can be used to gain a more comprehensive understanding of the diversity, mechanisms, and dynamics of fungal highway BFIs to provide valuable insights into the structural and functional dynamics within complex environments (e.g., soils) and across diverse habitats in which bacteria and fungi co-exist.

59 BASIC BIOLOGICAL SCIENCES↗
NASA NTRSDate not supplied

A Simulation Architecture for Air Traffic Over Urban Environments Supporting Autonomy Research in Advanced Air Mobility

NASA is conducting investigations into Advanced Air Mobility (AAM) concepts, aircraft, and operations. One of the most challenging scenarios for AAM will be enabling safe routine access into densely populated urban centers. To address challenges in the urban environment, a moderately high-fidelity simulation capability is needed to investigate AAM flight operations over a regional area for the development and evaluation of autonomy technologies. This paper describes a system to simulate flight operations around regions such as the San Francisco-Oakland Bay area at a moderately-high scale (10's-100's of flights) that incorporates detailed vehicle models and control necessary to support research in airborne autonomy. The flight vehicle utilizes NASA AAM concept vehicle dynamics integrated with a custom flight management system and flight control system to accurately simulate all phases of flight. Glass cockpit displays have been developed for monitoring aircraft operation over a detailed simulated urban environment. Simulation models have been integrated to simulate air and ground-based sensors, such as radar and LIDAR. The commercial X-Plane software package is used as a rendering engine to mimic vision-based sensors (such as onboard and ground-based cameras) at various times of day and in various weather conditions over a relatively detailed graphical model of the city. The paper presents a detailed illustration of the simulation and software architecture used for traffic over this urban region. This system is enabling the evaluation of NASA research concepts in autonomy for urban AAM operations on the path toward aircraft flight test evaluation.

Keerthana Kannan↗
NASA NTRSDate not supplied

Webinar on Urban Air Mobility Vision: Sky For All

The webinar will highlight the role UAM can play in future mobility improvements to enhance lives of people living in urban environments. In addition, challenges of integrating these vehicles into the current airspace will be discussed. NASA will present how these questions are being addressed from a US perspective.

Urban Air Mobility↗
DOE OSTI2020

Municipal Adaptation to Changing Curbside Demands: Findings from Semi-Structured Interviews with Ten U.S. Cities

Emerging mobility services (e.g. ride-hailing, e-commerce, micro-mobility, etc.), are generating novel and rapidly growing demands to use curbside space, with potentially large impacts on mobility, energy consumption, and related outcomes. This presents both opportunities and challenges to municipal agencies responsible for managing this interface between the road network and adjacent land uses, as legacy practices require updating. In this study, we employ a semi-structured interviewing approach to establish how municipalities are adapting to these new pressures on their curbside. We interviewed senior staff responsible for curbside policy of ten large U.S. municipalities, with populations ranging from ~250,000 to ~5,000,000 (and the majority of which are the central city of their metropolitan region). We document a trend of organizational restructuring to more formally include curbside management teams, with the majority of interviewees also reporting increased staffing. Respondents reported that operational failures at their curbside (e.g. demand in excess of capacity) have impacts on safety, capacity, and emergency vehicle mobility, with impacts highly concentrated spatially and temporally (e.g. late evenings in nightlife districts). We document a diversity of data flows between ride-hailing operators (e.g. Uber, Lyft) and municipalities, with some cities reporting obtaining types of data that other cities report not receiving, despite requesting such data. Finally, respondents consistently expressed a desire for new data streams and methodologies to help manage the curbside of the future. It is hoped that establishing the state of practice in this rapidly changing context will be of use to practitioners facing similar pressures as those of our interviewees.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗
NASA NTRS2023

An Interface Specification for Urban Air Mobility Performance Models to Support Air Traffic Management Research

Performance modeling of Urban Air Mobility vehicles in support of Air Traffic Management research poses new challenges. These aircraft often rely on a combination of fixed-wing and rotorcraft performance capabilities to enable a new and still-evolving concept of high-volume operations in densely populated areas. These characteristics lead to variability in performance model format and functionality, complicating model development and leading to difficulties for users integrating the models into their applications. This paper describes an interface specification for performance models that is intended to help address this issue. The interface aims to support the core functionality of performance models while also providing the necessary flexibility to both model developers and users. The specification describes the required model documentation, required inputs to the model, and required outputs from the model in general terms that are adaptable to most programming languages. The utilization of an appropriate interface specification will support the development of an Urban Air Mobility performance model database and help to improve interoperability of these models for a broad array of user applications.

Trajectory Prediction↗
NASA NTRS2023

An Interface Specification for Urban Air Mobility Performance Models to Support Air Traffic Management Research

Performance modeling of Urban Air Mobility vehicles in support of Air Traffic Management research poses new challenges. These aircraft often rely on a combination of fixed-wing and rotorcraft performance capabilities to enable a new and still-evolving concept of high-volume operations in densely populated areas. These characteristics lead to variability in performance model format and functionality, complicating model development and leading to difficulties for users integrating the models into their applications. This paper describes an interface specification for performance models that is intended to help address this issue. The interface aims to support the core functionality of performance models while also providing the necessary flexibility to both model developers and users. The specification describes the required model documentation, required inputs to the model, and required outputs from the model in general terms that are adaptable to most programming languages. The utilization of an appropriate interface specification will support the development of an Urban Air Mobility performance model database and help to improve interoperability of these models for a broad array of user applications.

Trajectory Prediction↗
DOE OSTI2019

A Semi-Empirical Framework for Interpreting Traveling Wave Ion Mobility Arrival Time Distributions

The inherent structural heterogeneity of biomolecules is an important biophysical property that is essential to their function, but is challenging to characterize experimentally. We present a workflow that rapidly and quantitatively assesses the conformational heterogeneity of peptides and proteins in the gas phase using traveling wave ion mobility (TWIM) arrival time distributions (ATDs). We have established a set of semi-empirical equations that model the TWIM ATD peak width and resolution across a wide range of wave amplitudes (V) and wave velocities (v). In addition, a conformational broadening parameter, δ, can be extracted from this analysis that reports on the contribution of conformational heterogeneity to the broadening of TWIM ATD peak width during ion mobility separation. We use this δ value to evaluate the conformational heterogeneity of a set of helical peptides, and our analysis correlates well with previous peak width observations reported for these ions. Furthermore, we use molecular dynamics simulations to independently investigate the general flexibility of these peptides in the gas phase, and generate similar trends found in experimental TWIM data. Finally, we extended our analysis to Avidin, a 64-kDa homotetramer, and quantify the structural heterogeneity of this intact complex using TWIM ATD data as a function of cross-linking. We observe an initial reduction in δ values as a function of cross-linker concentration, demonstrating the sensitivity of our δ value analysis to changes in flexibility of the assembly. .

74 ATOMIC AND MOLECULAR PHYSICS↗
NASA NTRS2018

Modeling of Orbital and Attitude Dynamics of Nanosatellites Controlled via Active Electrostatic Charging

The large-scale exploration of airless bodies, such as asteroids and moons, is gaining interest, however it is limited by mobility issues: the lack of atmosphere, low gravity, and unknown soil properties pose difficult challenges for many forms of traditional locomotion. The environment in proximity of these bodies is also electrically charged due to interactions with solar wind and UV radiation. The EGlider (Electrostatic Glider) concept would be able to overcome these mobility issues by leveraging the natural environment, allowing operations in close proximity of the surface, while enabling long duration missions by minimizing propellant consumption. The EGlider is an advanced concept for small satellite mobility and propulsion, which relies on the electric fields naturally present around airless bodies in order to generate forces and torques useful for maneuvering. It does so by extending electrically charged appendages, which enable it to electrostatically soar above the surface. By differentially charging its electrodes it can also produce torques to control its attitude. The charges are maintained by continuous active ion or electron emission from the spacecraft, in order to cancel out the neutralizing influx of charges from surrounding plasma. An investigation of the spacecraft-plasma interaction was carried out. This included studying the effect of electrode geometry and calculating the charge-to-mass ratios required to enable several mission scenarios. Long, thin wire electrodes were identified to be the most power-efficient and would allow power-to-weight ratios achievable with current nanosatellite technologies. High electrode potential represents the main limiting factor for the system design. In order to test the feasibility of active control by means of differential charging, a simple 2D interaction model was developed, and a feedback controller to stabilize the vehicle was tested in a simulation environment. The results confirmed that good performance could be obtained for both position and attitude control. Finally, a dedicated software was developed for future simulation and testing of control strategies for the EGlider. This software allows to study the trajectory and attitude of an arbitrarily configured spacecraft in the proximity of an arbitrarily defined main airless body. The spacecraft can be assembled from basic parts, each with specified electrical, mass and optical properties. Efficient models allow to calculate gravitational and electrical interactions with the rotating main body and the local plasma field, as well as solar radiation pressure effects. Control models can be implemented as simple plug-in functions and easily tested. The preliminary validation campaign showed good matching with the reference cases that have been analyzed.

Corpino, Sabrina↗
DOE OSTI2024

Exploring new frontiers in type 1 diabetes through advanced mass-spectrometry-based molecular measurements

Type 1 diabetes (T1D) is a devastating autoimmune disease for which advanced mass spectrometry (MS) methods are increasingly used to identify new biomarkers and better understand underlying mechanisms. For example, integration of MS analysis and machine learning has identified multimolecular biomarker panels. In mechanistic studies, MS has contributed to the discovery of neoepitopes, and pathways involved in disease development and identifying therapeutic targets. However, challenges remain in understanding the role of tissue microenvironments, spatial heterogeneity, and environmental factors in disease pathogenesis. Recent advancements in MS, such as ultra-fast ion-mobility separations, and single-cell and spatial omics, can play a central role in addressing these challenges. Here, in this work, we review recent advancements in MS-based molecular measurements and their role in understanding T1D.

60 APPLIED LIFE SCIENCES↗
NASA NTRSDate not supplied

Advanced Material Options for the Portable Life Support System

Meeting an aggressive mass requirement is a challenge for new space suit development efforts, including that of the Exploration Extravehicular Mobility Unit Portable Life Support System (PLSS) government reference design. To reduce overall system mass, titanium was selected as the primary metal for the PLSS backplate and thermal loop. However, galvanically compatible metals (Hastelloy, Inconel, Monel) have relatively high densities and require further design complexities such as coatings and more challenging manufacturing. Efforts to reduce the mass of the government reference design for an International Space Station (ISS) or lunar mission were halted due to the government’s transition to a commercial Extravehicular Activity (EVA) services approach, but research was conducted into methodologies for mass savings using advanced materials. With the advent of a lunar Artemis mission and potential for future Mars missions, mass becomes a more critical driver going forward. Therefore, alternative materials and processes must be considered to fully close the mass requirement. There are many new materials and processes that can be considered; however, considerations must be made to ensure galvanic compatibility, radiation concerns for sensitive electronics, vacuum compatibility, tight tolerances, fluid compatibility with oxygen and water, and thread insert options. Individual PLSS components may have different requirements and need to be considered separately for reducing overall mass. This report will touch on comparisons between advanced materials, focusing on composites, additive manufacturing, and plastics. It will also address the different processes that may need to be applied when using these materials in the harsh environment of space. Lastly, it will look at specific components and make recommendations for options to reduce the mass of each one.

Ryan Ogilvie↗
NASA NTRS2024

Advanced Material Options for the Portable Life Support System (PLSS)

Meeting an aggressive mass requirement is a challenge for new space suit development efforts, including that of the Exploration Extravehicular Mobility Unit Portable Life Support System (PLSS) government reference design. To reduce overall system mass, titanium was selected as the primary metal for the PLSS backplate and thermal loop. However, galvanically compatible metals (Hastelloy, Inconel, Monel) have relatively high densities and require further design complexities such as coatings and more challenging manufacturing. Efforts to reduce the mass of the government reference design for an International Space Station (ISS) or lunar mission were halted due to the government’s transition to a commercial Extravehicular Activity (EVA) services approach, but research was conducted into methodologies for mass savings using advanced materials. With the advent of a lunar Artemis mission and potential for future Mars missions, mass becomes a more critical driver going forward. Therefore, alternative materials and processes must be considered to fully close the mass requirement. There are many new materials and processes that can be considered; however, considerations must be made to ensure galvanic compatibility, radiation concerns for sensitive electronics, vacuum compatibility, tight tolerances, fluid compatibility with oxygen and water, and thread insert options. Individual PLSS components may have different requirements and need to be considered separately for reducing overall mass. This report will touch on comparisons between advanced materials, focusing on composites, additive manufacturing, and plastics. It will also address the different processes that may need to be applied when using these materials in the harsh environment of space. Lastly, it will look at specific components and make recommendations for options to reduce the mass of each one.

Ryan Ogilvie↗
DOE OSTI2018

Improved Sensitivity and Separations for Phosphopeptides using Online Liquid Chromotography Coupled with Structures for Lossless Ion Manipulations Ion Mobility–Mass Spectrometry

Phosphoproteomics greatly augments proteomics and holds tremendous potential for insights into the modulation of biological systems for various disease states, etc. However, numerous challenges hinder conventional methods in terms of measurement sensitivity, throughput, and capabilities for confident phosphopeptide and phosphosite identification. In this work, we report the first example of integrating structures for lossless ion manipulations ion mobility (SLIM) IM-MS with online reversed-phase liquid chromatography (LC) to evaluate its potential for addressing the aforementioned challenges. A mixture of 51 heavy-labeled phosphopeptides was analyzed with a SLIM IM module having integrated ion accumulation and long-path separation regions. The SLIM IM-MS provided improved sensitivity over an established LC-SRM method, with limits of detection as low as 50 pM (50 amol/µl) for several peptides. Conventionally problematic phosphopeptide isomers could be resolved following an 18 m SLIM IM separation. The 2-D peak capacity was estimated as ~9000 for 90 min LC separation coupled to an 18 m SLIM IM separation, considerably higher than LC alone and providing a basis for both improved identification and quantification, with additional gains projected with the future use of longer path SLIM IM separations. Thus, LC-SLIM IM-MS offers great potential for improving the sensitivity, separation, and throughput of phosphoproteomics analyses.

Chouinard, Christopher D.↗
NASA NTRS1992

High-temperature strain measurement techniques: Current developments and challenges

Since 1987, a very substantial amount of R&D has been conducted in an attempt to develop reliable strain sensors for the measurements of structural strains during ground testing and hypersonic flight, at temperatures up to at least 2000 deg F. Much of the effort has been focused on requirements of the NASP Program. This presentation is limited to the current sensor development work and characterization studies carried out within that program. It is basically an assessment as to where we are now and what remains to be done in the way of technical accomplishments to meet the technical challenges posed by the requirements and constraints established for the NASP Program. The approach for meeting those requirements and constraints has been multi-disciplinary in nature. It was recognized early on that no one sensor could meet all these requirements and constraints, largely because of the large temperature range (cryogenic to at least 2000 deg F) and many other factors, including the most challenging requirement that the sensor system be capable of obtaining valid 'first cycle data'. Present candidate alloys for resistance-type strain gages include Fe-Cr-Al and Pd-Cr. Although they have superior properties regarding withstanding very high temperatures, they exhibit large apparent strains that must either be accounted for or cancelled out by various techniques, including the use of a dual-element, half-bridge dummy gage, or electrical compensation networks. A significant effort is being devoted to developing, refining, and evaluating the effectiveness of those techniques over a broad range in temperature and time. In the quest to obtain first-cycle data, ways must be found to eliminate the need to prestabilize or precondition the strain gage, before it is attached to the test article. It should be noted that present NASP constraints do not permit prestabilization of the sensor, in situ. Gages are currently being 'heat treated' during manufacture in both the wire- and foil-type resistance strain gages, and evaluation is in progress. In addition, the 'gage-on-shim' concept is being revisited. That concept will permit heat treatment of the gage during manufacture, before attachment on the test article. Also, it may permit the individual calibration of each gage regarding gage factor and apparent strain. Candidate alloys for the NASP include titanium metal-matrix and carbon-carbon composites. Although those materials have very attractive properties at elevated temperatures in terms of strength and weight, they pose significant attachment problems. Methods for making reliable strain gage and thermocouple attachments to them are currently under development. Experience to date indicates that Rokide attachment of the sensor directly to the protective coating is easier than to the base material itself. However, interpreting strain data from gages attached in this way may prove difficult because of possible cracks in the coating that form 'islands' and the mobility of those 'islands'. It is concluded, therefore, that major technical challenges lie ahead as we proceed to meet the stringent strain sensor requirements and constraints of the NASP Program.

Lemcoe, M. M.↗
DOE OSTI2026

Overview of NLR Automated Mobility District Implementation Research - Phases I, II, and III. The Convergence of Automation, Electrification, and On-Demand Services: Enabling Resilient Automated Mobility Districts

A research program by the National Renewable Energy Laboratory has been investigating the implementation prospects for fully automated passenger transport systems that are deployed to operate within dense urban settings, referred to as Automated Mobility Districts (AMDs). An AMD emphasizes the deployment of automated vehicles (AV) passenger transport services within a dense urban setting and other major activity centers with intense passenger origin-destination demand patterns, such as those found in large business districts, airports, and university and medical campuses. Phase I and II surveyed 10 early deployment sites and subsequently collected and evaluated the lessons learned from these early deployment sites, with particular attention to fleet operations, impacts of service reliability, and vehicle technology evolution as the field of companies was being progressively winnowed by the challenges of full automation.

29 ENERGY PLANNING, POLICY, AND ECONOMY↗
DOE OSTI2018

Electron-Phonon Scattering in the Presence of Soft Modes and Electron Mobility in SrTiO 3 Perovskite from First Principles

Structural phase transitions and soft phonon modes pose a longstanding challenge to computing electron-phonon (e-ph) interactions in strongly anharmonic crystals. Here we develop a first-principles approach to compute e-ph scattering and charge transport in materials with anharmonic lattice dynamics. Our approach employs renormalized phonons to compute the temperature-dependent e-ph coupling for all phonon modes, including the soft modes associated with ferroelectricity and phase transitions. We show that the electron mobility in cubic SrTiO 3 is controlled by scattering with longitudinal optical phonons at room temperature and with ferroelectric soft phonons below 200 K. Our calculations can accurately predict the temperature dependence of the electron mobility in SrTiO 3 between 150-300 K, and reveal the microscopic origin of its roughly T -3 trend. Our approach enables first-principles calculations of e-ph interactions and charge transport in broad classes of crystals with phase transitions and strongly anharmonic phonons.

74 ATOMIC AND MOLECULAR PHYSICS↗
DOE OSTI2020

Gaussian process regression for radiological contamination mapping- Applied to optimal motion planning for mobile sensor platforms [Slides]

Want to achieve best representative characterization of the entire area efficiently and accurately-Unmanned aerial/ground vehicles (UAV/UGVs) for contamination mapping. Some major challenges include: Limited battery life (move smart), Human operated (fully autonomous controls) and Many measurements (predictive mapping capabilities). The objective: Develop fully autonomous controls for mobile sensor platforms to improve efficiency and maintain performance.

61 RADIATION PROTECTION AND DOSIMETRY↗
DOE OSTI2026

Toward Tunable Magnetic Dirac Semimetals: Mn Doping of Cd 3 As 2

Magnetic impurities provide a route toward increasing functionality in electronic materials, often enabling new device concepts and architectures. In the case of topological semimetals, dilute magnetic doping presents a particularly attractive approach for inducing a Dirac to Weyl phase change via time reversal symmetry breaking. However, efforts to realize changes in the electronic structure have been limited by challenges in incorporating magnetic impurities into crystals with sufficiently high electron mobilities to detect them via transport or spectroscopic techniques. Here, we demonstrate incorporation of Mn into Cd 3 ⁢As 2 Dirac semimetal thin films grown by molecular beam epitaxy (MBE). Using As-rich growth conditions and [001] oriented thin films, Mn compositions of >10% are achieved. Films contain uniform distributions of Mn with no evidence of secondary phases and exhibit electron mobilities greater than 10 000–30 000 cm 2 /Vs up to 5% Mn. An evolution in the magnetization behavior along with the emergence of a second quantum oscillation frequency at low Mn concentrations provide preliminary evidence of Mn-induced changes in the electronic structure that are consistent with a Weyl phase. This work demonstrates the potential of magnetically doping topological semimetal thin films and a pathway for synthesizing them.

36 MATERIALS SCIENCE↗