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DOE OSTI2024

Accelerated Irradiation and Qualification of Ceramic Nuclear Fuels

Accelerated irradiation efforts are an important topic in accelerated qualification of new nuclear fuel concepts for light water reactor (LWRs), microreactors, and other special purpose reactors. Accelerated fuel qualification combines both experimental and modeling work to expedite the total qualification time from approximately 20-25 years to 5-10 years timeframe. The scope covered under the current work package focuses on the experimental contributions for the neutron irradiations. Accelerated irradiation aims to reduce the total time needed for neutron irradiation to achieve targeted burnup, which is timely using conventional irradiation profiles. The purpose of this report is to detail the samples preparation for such irradiations, which contain standard, medium, and large grain undoped UO 2 , as well as large grain, Cr 2 O 3 -doped UO 2 . The data that will be generated from the irradiation of these samples will yield important information regarding fission gas retention as a function of grain size and the effect that Cr dopants have on diffusivity of fission gases.

11 NUCLEAR FUEL CYCLE AND FUEL MATERIALS↗

NASA NTRS1977

A model for microwave intensity propagation in an inhomogeneous medium

A combined analytic and phenomenological approach, utilizing Maxwell's equations in the Born approximation with radiative transfer theory, is used to describe the propagation of microwave intensity in a scattering medium characterized by three-dimensional random fluctuations in refractive index, as well as nonrandom variations in permittivity, temperature, and loss. This approach yields microwave intensities as a function of polarization, direction, and position. Numerical techniques are presented to solve the transport equations, which include cases of spatially varying coefficients, and highly peaked phase functions. Some computed results illustrating the behavior of microwave intensity in various media are presented. Included are the angular and frequency spectra of thermal emission from semi-infinite media, and the diffuse transmission and reflection response of a scattering layer. The effects of scatterer geometry and scale sizes; correlation function; and gradients in temperature, loss, and scattering parameters are also demonstrated. This model should be particularly useful in interpreting active and passive remote sensing data.

Fisher, A. D.↗

NASA NTRS1985

Simple equation to approximate the bidirectional reflectance from vegetative canopies and bare soil surfaces

A simple equation has been developed for describing the bidirectional reflectance of some vegetative canopies and bare soil surfaces. The equation describes directional reflectance as a function of zenith and azimuth view angles and solar azimuth angle. The equation works for simulated and field measured red and IR reflectance under clear sky conditions. Hemispherical reflectance can be calculated as a function of the simple equation coefficients by integrating the equation over the hemisphere of view angles. A single equation for estimating soil bidirectional reflectance was obtained using the relationships between solar zenith angles and the simple equation coefficients for medium and rough soil distributions. The equation has many useful applications such as providing a lower level boundary condition in complex plant canopy models and providing an additional tool for studying bidirectional effects on pointable sensors.

Walthall, C. L.↗

NASA NTRS1999

Characterization of Showerhead Performance At Low Pressure

The overall objective of this work is to characterize the flow to rough showerheads by deriving pressure drop versus velocity correlations to at can be then used in reactor scale simulations where the showerhead is approximated as a porous medium. At relatively low Reynolds numbers (less than 1-10 based on the hole length scale) and in the absence of slip flow, Darcy's Law, grad P = mu U/k, can be used to express the relation between the pressure drop and velocity where @mu@ is the fluid viscosity and it is the permeability that can be theoretically predicted as k= e R^2 /8, where e is the porosity. However, at sufficiently small hole diameters and decreased pressures (less than 5 Torr), the Knudsen number based on showerhead tube radius increases, and the flow may be in a transition regime. Different expressions have been proposed to account for this effect in the permeability by expressing k as a function of either pressure or Knudsen number. But at even higher Knudsen numbers, the pressure drop - velocity dependence is non-linear, and Darcy's Law no longer holds such that a permeability cannot be defined. The direct simulation Monte Carlo method is used along side conventional CFD techniques to determine the extent to which the CFD technique is appropriate and helps to derive correlations for the more rarefied cases of interest in these showerhead flows.

Hash, David B.↗

NASA NTRS2004

The Interaction of Hot and Cold Gas in the Disk and Halo of Galaxies

Most of the thermal energy in the Galaxy and perhaps most of the baryons in the Universe are found in hot (log T approximately 5.5 - 7) gas. Hot gas is detected in the local interstellar medium, in supernova remnants (SNR), the Galactic halo, galaxy clusters and the intergalactic medium (IGM). In our own Galaxy, hot gas exists in large superbubbles up to several hundred pc in diameter that locally dominate the interstellar medium (ISM) and determine its thermal and dynamic evolution. While X-ray observations using ROSAT, Chandra and XMM have allowed us to make dramatic progress in mapping out the morphology of the hot gas and in understanding some of its spectral characteristics, there remain fundamental questions that are unanswered. Chief among these questions is the way that hot gas interacts with cooler phase gas and the effects these interactions have on hot gas energetics. The theoretical investigations we proposed in this grant aim to explore these interactions and to develop observational diagnostics that will allow us to gain much improved information on the evolution of hot gas in the disk and halo of galaxies. The first of the series of investigations that we proposed was a thorough exploration of turbulent mixing layers and cloud evaporation. We proposed to employ a multi-dimensional hydrodynamical code that includes non-equilibrium ionization (NEI), radiative cooling and thermal conduction. These models are to be applied to high velocity clouds in our galactic halo that are seen to have O VI by FUSE (Sembach et ai. 2000) and other clouds for which sufficient constraining observations exist.

Slavin, Jonathan↗

NASA NTRSDate not supplied

4pPA1 - Turbulence Effects on Shaped Booms: Central Composite Design of Modeled Atmospheric Turbulence Parameters for Sonic Boom Propagation

Propagation of sonic booms through turbulence reduces mean sonic boom perception metric levels and also causes considerable variability. NASA’s PCBoom suite of sonic boom acoustic propagation modules includes an approximate method for accounting for the effects of turbulence on traditional N-wave sonic booms. The current implementation is ineffective for shaped sonic booms or low-booms, and it also has limited values for turbulence and ambient input parameters. NASA’s future X-59 low-boom community noise surveys require an accurate estimate of the effects of turbulence in regions across the USA, so the module must be improved. This work presents the methods of selecting which ambient and turbulence parameters should be included in an improved PCBoom turbulence module. Turbulence and ambient data were collected from two atmospheric model databases, the Climate Forecast System Version 2 and European Centre for Medium-Range Weather Forecast Reanalysis Version 5 (ERA5), hourly from 7 AM to 7 PM local time for 10 years at 19 locations across the USA. A fully-factorial propagation analysis using these parameters would be exceedingly computationally expensive. Instead, a central composite design was chosen resulting in 45 combinations of ambient and turbulence parameters. These 45 cases effectively sample the space balancing computational burden.

turbulence↗

NASA NTRS2022

Design of Hypervelocity Flow Generator (HFG) and Its Diagnostics

Ground facilities for hypersonic research are a key element for successful development of aerodynamically proven hypervelocity vehicles. Design concepts and diagnostics of a hypervelocity flow generator (HFG) were made as a test platform for hypersonic/hypervelocity spacecraft models at the NASA Langley Research Center. The HFG is a hypersonic flow field generator using optically heated gas which is blown into an 80 cu m vacuum chamber. The vacuum chamber is kept at a stable vacuum pressure with a combination of three large vacuum pumps, while the HFG is in the test mode. The HFG provides a relatively small test section with approximately a 20 cm window. This facility was designed to generate 2.45 km/sec of flow speed, and potentially generate a continuous flow with the nozzle and vacuum system. The window in test section provides a direct view of the shock wave around a model in order to measure temperature, pressure, and density profile within the shock layer. One of the key test goals under this project is to understand why the emission spectra from the standing shockwave plasma predicted by Lora-Loran codes are significantly different from the measured emission spectra from the Flight Investigation Reentry Environment (FIRE II) Flight. The correct estimate of the thermal loading on the leading edge of hypersonic vehicles greatly affects the aerodynamic design, the material selection for the vehicle, and the cooling requirement and can be obtained by the precise modeling of emission spectra from shockwave plasma. However, the estimation of thermal loading is not an easy task due to complex non-equilibrium radiative process within high temperature shock layers that still falls into a category of cold plasma. Direct flight experiments are the most desirable, but not a cost-effective approach. Analysis by computational fluid dynamics (CFD) offers many test flexibilities. However, the CFD codes must be fully tested and validated with experimental data before the codes are effectively used for practical design. Large discrepancies between experiments and codes appear in hypersonic/hypervelocity flow regimes at high altitudes of 60 km ~ 90 km. This HFG facility offers some important parameters for CFD code validation, such as collision cross-sections, relaxation times, reaction rate coefficients and transportation coefficients. The HFG test facility is based on the ejection flow of high temperature gas heated over to 3500 K through a nozzle. The tungsten gas chamber of the HFG is heated up to a desired temperature by a 60 kW optical power beam source. This system consists of an optical power source, a thermal chamber, an expansion nozzle, a test section, and an 80 cu m vacuum tank. 60 kW optical input power is obtained from the 150 kW Vortek arc lamp system (by Vortek Industries, Vancouver, Canada). This optical beam is focused to heat the gas chamber within which a flow media is heated. The maximum achievable temperature of the flow medium reached approximately 3500 K or even higher but is limited by the melting point of the chamber material used. The exhaust velocity through the nozzle was determined by the stagnation temperature and the molecular weight of the working medium at the test section. To provide design parameters, a NASA Chemical Equilibrium with Application (CEA) computer program is used for the simulation of aerothermal data. This CEA program can calculate chemical equilibrium and properties of complex mixtures using shock tube parameters. For nitrogen gas at 2666 K stagnation temperature, the maximum achievable velocity at the test section is approximately 2.45 km/sec which is within the range of the thermal velocity of 8000 K shock layer. Based on the calculation through the CEA program, the design parameters of a HFG were determined and implemented for the test section that includes an expansion nozzle. The installed test section of the experimental facility can sustain a condition of a re-entry vehicle from the Space at an altitude of 60-90 km. After installation of the HFG, the system was fully tested and its operational parameters were measured. An 80 cu m vacuum chamber of HFG was set at 1 torr level to keep a stable downstream condition. The pumping time to reach the minimum vacuum pressure (~ 1 torr) at the test chamber from atmospheric pressure was approximately an hour using Kinney (MBV-14000/MB -1600/KT-300) and Stokes (Model 1772 and 412) pumps. Such a setup condition allowed a continued stable operation of the HFG experiment with flowrates through 1-, 3-, or 5-mm diameters of nozzle throat. The flow characteristics of the HFG for various operating conditions were performed using a focused Schlieren method. At 0.8 torr chamber pressure, a barrel shock was observed at the test section. With a sphere obstacle of 12 mm diameter in the flow, a bow shock (~ 2-mm thickness) was observed by a focused Schlieren visualization method.

Hypervelocity flow↗

DOE OSTI2024

Effectiveness of denoising diffusion probabilistic models for fast and high-fidelity whole-event simulation in high-energy heavy-ion experiments

Artificial intelligence (AI) generative models, such as generative adversarial networks (GANs), variational autoencoders, and normalizing flows, have been widely used and studied as efficient alternatives for traditional scientific simulations. However, they have several drawbacks, including training instability and inability to cover the entire data distribution, especially for regions where data are rare. This is particularly challenging for whole-event, full-detector simulations in high-energy heavy-ion experiments, such as sPHENIX at the Relativistic Heavy Ion Collider and Large Hadron Collider experiments, where thousands of particles are produced per event and interact with the detector. This work investigates the effectiveness of denoising diffusion probabilistic models (DDPMs) as an AI-based generative surrogate model for the sPHENIX experiment that includes the heavy-ion event generation and response of the entire calorimeter stack. DDPM performance in sPHENIX simulation data is compared with a popular rival, GANs. Results show that both DDPMs and GANs can reproduce the data distribution where the examples are abundant (low-to-medium calorimeter energies). Nonetheless, DDPMs significantly outperform GANs, especially in high-energy regions where data are rare. Additionally, DDPMs exhibit superior stability compared to GANs. The results are consistent between both central and peripheral centrality heavy-ion collision events. Moreover, DDPMs offer a substantial speedup of approximately a factor of 100 compared to the traditional Geant4 simulation method.

42 ENGINEERING↗

NASA NTRS1993

Thermal infrared emission spectroscopy of natural surfaces: Application to desert varnish coatings on rocks

Thermal infrared spectroscopy has become an increasingly important tool for remote compositional analysis and geologic mapping. Most published laboratory measurements have been obtained in bidirectional reflection or transmission, whereas remotely sensed thermal infrared data are obtained by measuring the emitted energy. Section 2 of this paper describes a laboratory technique for determining calibrated emissivities of natural surfaces. Equations are developed to account for the energy reflected from the environment and to determine directly the sample temperature from measurements of hot and cold blackbody targets. Two methods for determining emissivity are developed: one in which only a hot sample measurement is made and the reflected background energy is removed by modeling, and a second in which the sample is cooled and the reflected energy is measured directly. Relative emissivity can be obtained to approximately 1% and absolute emissivities can be obtained to 2-15%, depending on the validity of the assumption that the emissivity of the sample is unity at some wavelength. The emission data agree well within the hemispherically integrated reflection data but point out probelms associated with bidirectional reflectance measurements. Section 3 applies emissivity measurements to the study of layered surfaces consisting of desert varnish coatings on granite and granodiorite rock suites. Two linear models are developed: the first assumes linear mixing of independent emission from the substrate and varnish (checkerboard model); the second models tansmission through an absorbing/emitting medium. Regardless of whether the varnish is or is not relatively transparant and strongly absorptive, the spectral effect of varnish increases linearly with varnish thickness, indicating that thick patches of varnish dominate the spectral properties. As a result, medium varnish thickness can be determined from spectral measurements. In addition, the composition of a substrate can be estimated through varnish layers up to 40-50 micrometers in median thickness, and the composition of the varnish material can be determined if the substrate material is known. The varnishes studied are composed primarily of clay materials, consistent with previous studies of varnish composition.

Christensen, Philip R.↗

NASA NTRS1995

Near-infrared (Fe II) and Pa Beta imaging and spectroscopy of Arp 220

We have imaged the ultraluminous infrared galaxy Arp 220 in light of the near-infrared (Fe II) 1.257 micron and Pa-beta lines, and have obtained spectra in the J- and H-band atmospheric windows. Arp 220 is a strong source of (Fe II) and Pa-beta emission, with luminosities of 1.3 x 10(exp 41) and 9.2 x 10(exp 40) ergs/s, respectively. The (Fe II) and Pa-beta emission are both extended over the central 2 sec-3 sec, but with different morphologies. We suggest that the extended (Fe II) emission is produced through the interaction of fast shocks with ambient gas in the interstellar medium (ISM) at the base of the outflowing, supernovae-driven superwind mapped by Heckman et al. (1987). The bolometric luminosity of the starburst required to power this wind is estimated to be at least 2 x 10(exp 11) solar luminosity. If the spatially unresolved (Fe II) emission is produced via a large number of supernova remnants, the implied rate is approximately 0.6/yr. The overall luminosity of such a starburst could account for a large fraction (1/2-1/3) of the Arp 220 energy budget, but the large deficit of ionizing photons (as counted by the Pa-beta luminosity) requires that the starburst be rapidly declining and/or have a low upper mass cutoff. Alternatively, dust may effectively compete with the gas for ionizing photons, or much of the ionizing radiation may escape through 'holes' in the ISM. It is also possible that a buried active galactic nuclei (AGN) produces a large fraction of the unresolved (Fe II) and Pa-beta emission. We briefly discuss these possibilities in light of these new imaging and spectroscopic data.

Armus, L.↗

NASA NTRS1999

Bidirectional Reflectance of a Macroscopically Flat, High-Albedo Particulate Surface: An Efficient Radiative Transfer Solution and Applications to Regoliths

Many remote sensing applications rely on accurate knowledge of the bidirectional reflection function (BRF) of surfaces composed of discrete, randomly positioned scattering particles. Theoretical computations of BRFs for plane-parallel particulate layers are usually reduced to solving the radiative transfer equation (RTE) using one of existing exact or approximate techniques. Since semi-empirical approximate approaches are notorious for their low accuracy, violation of the energy conservation law, and ability to produce unphysical results, the use of numerically exact solutions of RTE has gained justified popularity. For example, the computation of BRFs for macroscopically flat particulate surfaces in many geophysical publications is based on the adding-doubling (AD) and discrete ordinate (DO) methods. A further saving of computer resources can be achieved by using a more efficient technique to solve the plane-parallel RTE than the AD and DO methods. Since many natural particulate surfaces can be well represented by the model of an optically semi-infinite, homogeneous scattering layer, one can find the BRF directly by solving the Ambartsumian's nonlinear integral equation using a simple iterative technique. In this way, the computation of the internal radiation field is avoided and the computer code becomes highly efficient and very accurate and compact. Furthermore, the BRF thus obtained fully obeys the fundamental physical laws of energy conservation and reciprocity. In this paper, we discuss numerical aspects and the computer implementation of this technique, examine the applicability of the Henyey-Greenstein phase function and the sigma-Eddington approximation in BRF and flux calculations, and describe sample applications demonstrating the potential effect of particle shape on the bidirectional reflectance of flat regolith surfaces. Although the effects of packing density and coherent backscattering are currently neglected, they can also be incorporated. The FORTRAN implementation of the technique is available on the World Wide Web, and can be applied to a wide range of remote sensing problems. BRF computations for undulated (macroscopically rough) surfaces are more complicated and often rely on time consuming Monte Carlo procedures. This approach is especially inefficient for optically thick, weakly absorbing media (e.g., snow and desert surfaces at visible wavelengths since a photon may undergo many internal scattering events before it exists the medium or is absorbed. However, undulated surfaces can often be represented as collections of locally flat tilted facets characterized by the BRF found from the traditional plane parallel RTE. In this way the MOnte Carlo procedure could be used only to evaluate the effects of surface shadowing and multiple surface reflections, thereby bypassing the time-consuming ray tracing inside the medium and providing a great savings of CPU time.

Mishchenko, Michael I.↗

DOE OSTI2022

Insights into Sorption and Molecular Transport of Aqueous Glucose into Zeolite Nanopores

Liquid-phase heterogeneous catalysis using zeolites is important for biomass conversion to fuels and chemicals. There is a substantial body of work on gas-phase sorption in zeolites with different topologies; however, studies investigating the diffusion of complex molecules in liquid medium into zeolitic nanopores are scarce. Here, we present a molecular dynamics study to understand the sorption and diffusion of aqueous β-d-glucose into β-zeolite silicate at T = 395 K and P = 1 bar. Through 2-μs-long molecular dynamics trajectories, we reveal the role of the solvent, the kinetics of the pore filling, and the effect of the water model on these properties. We find that the glucose and water loading is a function of the initial glucose concentration. Although the glucose concentration increases monotonically with the initial glucose concentration, the water loading exhibits a nonmonotonic behavior. At the highest initial concentration (~20 wt %), we find that the equilibrium loading of glucose is approximately five molecules per unit cell and displays a weak dependence on the water model. Glucose molecules follow a single-file diffusion in the nanopores due to confinement. Here, the dynamics of glucose and water molecules slows significantly at the interface. The average residence time for glucose molecules is an order of magnitude larger than that in the bulk solution, while it is about twice as large for the water molecules. Our simulations reveal critical molecular details of the glucose molecule’s local environment inside the zeolite pore relevant to catalytic conversion of biomass to valuable chemicals.

09 BIOMASS FUELS↗

DOE OSTI2024

Optical second harmonic generation in anisotropic multilayers with complete multireflection of linear and nonlinear waves using ♯SHAARP.ml package

Abstract Optical second harmonic generation (SHG) is a nonlinear optical effect widely used for nonlinear optical microscopy and laser frequency conversion. Closed-form analytical solution of the nonlinear optical responses is essential for evaluating materials whose optical properties are unknown a priori. A recent open-source code, ♯SHAARP. si , can provide such closed form solutions for crystals with arbitrary symmetries, orientations, and anisotropic properties at a single interface. However, optical components are often in the form of slabs, thin films on substrates, and multilayer heterostructures with multiple reflections of both the fundamental and up to ten different SHG waves at each interface, adding significant complexity. Many approximations have therefore been employed in the existing analytical approaches, such as slowly varying approximation, weak reflection of the nonlinear polarization, transparent medium, high crystallographic symmetry, Kleinman symmetry, easy crystal orientation along a high-symmetry direction, phase matching conditions and negligible interference among nonlinear waves, which may lead to large errors in the reported material properties. To avoid these approximations, we have developed an open-source package named Second Harmonic Analysis of Anisotropic Rotational Polarimetry in Multilayers (♯SHAARP. ml ). The reliability and accuracy are established by experimentally benchmarking with both the SHG polarimetry and Maker fringes using standard and commonly used nonlinear optical materials as well as twisted 2-dimensional heterostructures.

36 MATERIALS SCIENCE↗

NASA NTRS1974

Isotopic composition of low energy beryllium in the galactic cosmic rays

The multielement charged-particle telescope on the IMP-5 satellite obtained data essentially continuously for more than three years in the interplanetary medium under stable instrument conditions which have made it possible to separate the H, He, and Be isotopes in the galactic cosmic radiation using the technique of double dE/dx vs residual E and particle range measurements. Special emphasis is placed on demonstrating that in the energy range from 50 to 150 MeV/nucleon the instrument clearly resolved Be-7 vs Be-9. A detailed analysis of approximately 100 Be events collected over the mission lifetime yields the ratios (Be-7/Be) = 0.50 plus or minus 0.07, (Be-9/Be) = 0.41 plus or minus 0.10, and (Be-10/Be) = 0.09 plus or minus 0.10. These results are consistent with current models of galactic cosmic ray propagation after taking account of the effects of solar modulation.

Garcia-Munoz, M.↗

NASA NTRS1990

Somatic proembryo production from excised, wounded zygotic carrot embryos on hormone-free medium: evaluation of the effects of pH, ethylene and activated charcoal

Wounded zygotic embryos of cultivated carrot produce somatic proembryos on hormone-free nutrient medium containing 1 mM NH4+ as the sole nitrogen source. Continued maintenance of proembryos on this medium leads to a "pure" culture of preglobular stage proembryos (PGSPs). Ethylene had no effect on this process. Also, somatic embryo production was not affected by growing cultures on activated charcoal-impregnated filter papers. However, somatic proembyros initiated on activated charcoal papers were not maintainable as PGSPs and developed into later embryo stages. Normally, medium pH dropped from 5.7 to 4 during each subculture period, but when using activated charcoal papers the pH endpoint was around 6 - 7 due to a leachable substance(s) within the filter papers. When powdered, activated charcoal was used in the medium as an adsorbent of products potentially released after wounding, pH dropped at the normal rate and to the expected levels; proembryos did not mature into later embryo stages and were maintainable exclusively as PGSPs. Low pH (approximately 4) is detrimental to proembyro production, but is essential to maintaining PGSPs on hormone-free nutrient medium, whereas a sustained pH > or = 5.7 allows continued development of PGSPs into later embryo stages.

NASA Program Space Biology↗

NASA NTRS1972

Solar modulation of galactic cosmic rays. 3: Implications of the Compton-Getting coefficient

Spectra of modulated galactic cosmic rays are expressed in terms of the Compton-Getting coefficient C. This parameter can reveal the energy range over which the force field approximation is valid, and the range where convection effects dominate those of diffusion. A value of C near zero over an extended low energy range implies that the radial gradient at low energies can not be large. This small gradient may imply that the diffusion coefficient increases beyond 1 AU less rapidly than proportionally to heliocentric radial distance, and/or there is essentially no scattering for a sizeable distance from the Sun to earth. The behavior of C with rigidity (or energy) is discussed in terms of the omnidirectional distribution function f sub zero. Contours of constant f sub zero in the heliocentric distance vs rigidity plane are useful for illustrating the mean rigidity loss experienced by cosmic rays in the interplanetary medium.

Fisk, L. A.↗

NASA NTRS1975

Far infrared maser communications technology

An optically pumped FIR laser was constructed and tested. Optimum operating conditions were determined with CH3OH as the lasing medium. The laser was found to operate equally well with flowing gas or in a sealed off configuration. The FIR cavity stability and pump laser stability were found to have significant problems. The absorption coefficient per unit pressure of 1-1 difluoroethylene at the P(22) and P(24) lines of the 10.4 micron CO2 band was measured. The FIR line pumped by P(22) occurs at approximately 890 microns, which may be in an atmospheric transmission window. It was found that significant Stark tuning of absorption lines of methanol and 1-1 difluoroethylene can be accomplished, even at the usual 100 to 300 mTorr operating pressures of FIR lasers. This means that the use of Stark tuning may enable more effective use of pump laser output.

Claspy, P. C.↗

NASA NTRS1982

Studies with sample conductivity, insertion rates, and particle deflection in a continuous flow electrophoresis system

The continuous flow electrophoresis system makes electrophoresis possible in a free-flowing film of aqueous electrolyte medium. The sample continuously enters the electrolyte at the top of the chamber and is subjected to the action of a lateral dc field. This divides the sample into fractions since each component has a distinctive electrophoretic mobility. Tests were made using monodisperse polystyrene latex microspheres to determine optimum sample conductivity, insertion rates and optimum electric field applications as baseline data for future STS flight experiments. Optimum sample flow rates for the selected samples were determined to be approximately 26 micro-liters/min. Experiments with samples in deionized water yielded best results and voltages in the 20 V/cm to 30 V/cm range were optimum. Deflections of formaldehyde fixed turkey and bovine erythrocytes were determined using the continuous flow electrophoresis system. The effects of particle interactions on sample resolution and migration in the chamber was also evaluated.

Williams, G., Jr.↗