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At least 343 records · Page 19
All sourcesNASA NTRSDOE OSTI
NASA NTRS2016

Mechanotransductive Regulation of Gap-Junction Activity Between MLO-Y4 Osteocyte-Like and MC3T3-E1 Osteoblast-Like Cells in Three-Dimensional Co-Culture.

Cell and animal studies conducted onboard the International Space Station and formerly on Shuttle flights have provided groundbreaking data illuminating the deleterious biological response of bone to mechanical unloading. However the intercellular communicative mechanisms associated with the regulation of bone synthesis and bone resorption cells are still largely unknown. Connexin-43 (CX43), a gap junction protein, is hypothesized to play a significant role in osteoblast and osteocyte signaling. The purpose of this investigation was to evaluate within a novel three-dimensional microenvironment how the osteocyte-osteoblast gap-junction expression changes when cultures are exposed to exaggerated mechanical load. MLO-Y4 osteocyte-like cells were cultured on a 3D-Biotek polystyrene insert and placed in direct contact with an MC3T3-E1 pre-osteoblast co-cultured monolayer and exposed to 48 h of mechanical stimulation (pulsatile fluid flow (PFF) or monolayer cyclic stretch (MCS)) then evaluated for viability, proliferation, metabolism, and CX43 expression. Mono-cultured MLO-Y4 and MC3T3-E1 control experiments were conducted under PFF and MCS stimulation to observe how strain application stimuli (PFF cell membrane shear or MCS cell focal adhesion/attachment loading) initiates different signaling pathways or downstream regulatory controls. TotalLive cell count, viability and metabolic reduction (Trypan Blue, LIVEDead and Alamar Blue analysis respectively) indicate that mechanical activation of MC3T3-E1 cells inhibits proliferation while maintaining an average 1.04E4 reductioncell metabolic rate, *p0.05 n4. MLO-Y4s in monolayer culture increase in number when exposed to MCS loading but the percent of live cells within the population is low (46.3 total count, *p0.05 n4), these results may indicate an apoptotic signaling cascade. PFF stimulation of the three-dimensional co-cultures elicits a universal increase in CX43 in MLO-Y4 and MC3T3-E1 cells, illustrated by immunohistological observation. Increased CX43 expression is also observed with the three-dimensional co-cultures with MC3T3-E1 MCS stimulation but the increased gap-junction protein presence was limited to the osteoblast-osteocyte interface region. Previously reported PCR evaluation of osteogenic markers further corroborate that the co-cultured populations communicative networks play a role in translating mechanical signals to molecular messaging. These findings suggests an osteocyte-osteoblast gap-junction signaling feedback mechanism may regulate mechanotransduction of apoptosis initiation and transcription of cytokine signaling proteins responsible for stem cell niche recruitment much more directly than previously believed.

mechanical loading↗
NASA NTRS2017

A Review of the Proposed K (sub Isi) Offset-Secant Method for Size-Independent Linear-Elastic Toughness Evaluation

The proposed size-independent linear-elastic fracture toughness, K (sub Isi), for potential inclusion in ASTM E399 targets a consistent 0.5 millimeters crack extension for all specimen sizes through an offset secant that is a function of the specimen ligament length. The K (sub Isi) method also includes an increase in allowable deformation, and the removal of the P (sub max)/P (sub Q) criterion. A finite element study of the K (sub Isi) test method confirms the viability of the increased deformation limit, but has also revealed a few areas of concern. Findings: 1. The deformation limit, b (sub o) greater than or equal to 1.1 times (K (sub I) divided by delta (sub ys) squared) maintains a K-dominant crack tip field with limited plastic contribution to the fracture energy; 2. The three dimensional effects on compliance and the shape of the force versus CMOD (Crack-Mouth Opening Displacement) trace are significant compared to a plane strain assumption; 3. The non-linearity in the force versus CMOD trace at deformations higher than the current limit of 2.5 times (K (sub I) divided by delta (sub ys) squared) is sufficient to introduce error or even "false calls" regarding crack extension when using a constant offset secant line. This issue is more significant for specimens with W (width) greater than or equal to 2 inches; 4. A non-linear plasticity correction factor in the offset secant may improve the viability of the method at deformations between 2.5 times (K (sub I) divided by delta (sub ys) squared) and 1.1 times (K (sub I) divided by delta (sub ys) squared).

James, Mark↗
NASA NTRS2017

Unmanned Aircraft Systems (UAS) Integration in the National Airspace System (NAS) Project KDP-C Review

The topics discussed are the UAS-NAS project life-cycle and ARMD thrust flow down, as well as the UAS environments and how we operate in those environments. NASA's Armstrong Flight Research Center at Edwards, CA, is leading a project designed to help integrate unmanned air vehicles into the world around us. The Unmanned Aircraft Systems Integration in the National Airspace System project, or UAS in the NAS, will contribute capabilities designed to reduce technical barriers related to safety and operational challenges associated with enabling routine UAS access to the NAS. The project falls under the Integrated Systems Research Program office managed at NASA Headquarters by the agency's Aeronautics Research Mission Directorate. NASA's four aeronautics research centers - Armstrong, Ames Research Center, Langley Research Center, and Glenn Research Center - are part of the technology development project. With the use and diversity of unmanned aircraft growing rapidly, new uses for these vehicles are constantly being considered. Unmanned aircraft promise new ways of increasing efficiency, reducing costs, enhancing safety and saving lives 460265main_ED10-0132-16_full.jpg Unmanned aircraft systems such as NASA's Global Hawks (above) and Predator B named Ikhana (below), along with numerous other unmanned aircraft systems large and small, are the prime focus of the UAS in the NAS effort to integrate them into the national airspace. Credits: NASA Photos 710580main_ED07-0243-37_full.jpg The UAS in the NAS project envisions performance-based routine access to all segments of the national airspace for all unmanned aircraft system classes, once all safety-related and technical barriers are overcome. The project will provide critical data to such key stakeholders and customers as the Federal Aviation Administration and RTCA Special Committee 203 (formerly the Radio Technical Commission for Aeronautics) by conducting integrated, relevant system-level tests to adequately address safety and operational challenges of national airspace access by unmanned aircraft systems, or UAS. In the process, the project will work with other key stakeholders to define necessary deliverables and products to help enable such access. Within the project, NASA is focusing on five sub-projects. These five focus areas include assurance of safe separation of unmanned aircraft from manned aircraft when flying in the national airspace; safety-critical command and control systems and radio frequencies to enable safe operation of UAS; human factors issues for ground control stations; airworthiness certification standards for UAS avionics and integrated tests and evaluation designed to determine the viability of emerging UAS technology. Five Focus Areas of the UAS Integration in the NAS Project Separation Assurance Provide an assessment of how planned Next Generation Air Transportation System (NextGen) separation assurance systems, with different functional allocations, perform for UAS in mixed operations with manned aircraft Assess the applicability to UAS and the performance of NASA NextGen separation assurance systems in flight tests with realistic latencies and uncertain trajectories Assess functional allocations ranging from today's ground-based, controller-provided aircraft separation to fully autonomous airborne self-separation Communications Develop data and rationale to obtain appropriate frequency spectrum allocations to enable safe and efficient operation of UAS in the NAS Develop and validate candidate secure safety-critical command and control system/subsystem test equipment for UAS that complies with UAS international/national frequency regulations, standards and recommended practices and minimum operational and aviation system performance standards for UAS Perform analysis to support recommendations for integration of safety-critical command and control systems and air traffic control communications to ensure safe and efficient operation of UAS in the NAS Human Systems Integration Develop a research test bed and database to provide data and proof of concept for GCS - ground control station - operations in the NAS Coordinate with standards organizations to develop human-factors guidelines for GCS operation in the NAS Certification Define a UAS classification scheme and approach to determining Federal Aviation Regulation airworthiness requirements applicable to all UAS digital avionics Provide hazard and risk-related data to support development of type design criteria and best development practices Integrated Tests and Evaluation Integrate and test mature concepts from technical elements to demonstrate and test viability Evaluate the performance of technology development in a relevant environment (full-mission, human-in-the-loop simulations and flight tests)

outreach↗
NASA NTRS2017

Growth and Survival of Perchlorate-Reducing Bacteria in Media Containing Elevated Perchlorate Concentrations and UV-C Conditions

Introduction: The identification of perchlorate (ClO4(-)) on Mars has led to the possibility that complete redox couples are available for microbial metabolism in contemporary surface environments. Perchlorate-reducing bacteria (PRB) utilize ClO4(-) and chlorate (ClO3(-)) as terminal electron acceptors due to the high reduction potential. Additionally, ClO4(-) salts have been suggested as a possible source of brines on Mars and spectral evidence indicates that the hydration of ClO4(-) salts in the regolith of Martian is linked to the surface recurring slope lineae (RSL). For these reasons PRB may serve as analog organisms for possible life on Mars. However, there is very little information on the viability of PRB in aqueous environments that contain high levels of perchlorate Microorganisms on or near the surface of Mars, such as in the RSL, would potentially be exposed to high-salinity and high ultraviolet radiation environments. Under these extreme conditions, microorganisms must possess mechanisms for maintaining continued high genome fidelity. To assess possible microbial viability in contemporary Mars analog environments we are investigating the tolerance of two PRB strains in aqueous conditions under high UV-C conditions and high ClO4(-) concentrations.

Bywaters, K. F.↗
NASA NTRS2017

Building Better Biosensors for Exploration into Deep-Space, Using Humanized Yeast

1.BioSentinel is 1 of 13 secondary payloads hitching a ride beyond Low Earth Orbit on Exploration Mission 1 (EM-1), set to launch from NASAs Space Launch System in 2019. EM-1 is our first opportunity to investigate the effects of the deep space environment on a eukaryotic biological system, the budding yeast S. cerevisiae. Though separated by a billion years of evolution we share hundreds of genes important for basic cell function, including responses to DNA damage. Thus, yeast is an ideal biosensor for detecting typesextent of damage induced by deep-space radiation.We will fly desiccated cells, then rehydrate to wake them up when the automated payload is ready to initiate the experiment. Rehydration solution contains SC (Synthetic Complete) media and alamarBlue, an indicator for changes in growth and metabolism. Telemetry of LED readings will then allow us to detect how cells respond throughout the mission. The desiccation-rehydration process can be extremely damaging to cells, and can severely diminish our ability to accurately measure and model cellular responses to deep-space radiation. The aim of this study is to develop a better biosensor: yeast strains that are more resistant to desiccation stress. We will over-express known cellular protectants, including hydrophilin Sip18, the protein disaggregase Hsp104, and thioredoxin Trx2, a responder to oxidative stress, then measure cell viability after desiccation to determine which factors improve stress tolerance. Over-expression of SIP18 in wine yeast starter cultures was previously reported to increase viability following desiccation stress by up to 70. Thus, we expect similar improvements in our space-yeast strains. By designing better yeast biosensors we can better prepare for and mitigate the potential dangers of deep-space radiation for future missions.This work is funded by NASAs AES program.

biosensor↗
NASA NTRS2017

Biosentinel: Improving Desiccation Tolerance of Yeast Biosensors for Deep-Space Missions

BioSentinel is one of 13 secondary payloads to be deployed on Exploration Mission 1 (EM-1) in 2019. We will use the budding yeast Saccharomyces cerevisiae as a biosensor to determine how deep-space radiation affects living organisms and to potentially quantify radiation levels through radiation damage analysis. Radiation can damage DNA through double strand breaks (DSBs), which can normally be repaired by homologous recombination. Two yeast strains will be air-dried and stored in microfluidic cards within the payload: a wild-type control strain and a radiation sensitive rad51 mutant that is deficient in DSB repairs. Throughout the mission, the microfluidic cards will be rehydrated with growth medium and an indicator dye. Growth rates of each strain will be measured through LED detection of the reduction of the indicator dye, which correlates with DNA repair and the amount of radiation damage accumulated. Results from BioSentinel will be compared to analog experiments on the ISS and on Earth. It is well known that desiccation can damage yeast cells and decrease viability over time. We performed a screen for desiccation-tolerant rad51 strains. We selected 20 re-isolates of rad51 and ran a weekly screen for desiccation-tolerant mutants for five weeks. Our data shows that viability decreases over time, confirming previous research findings. Isolates L2, L5 and L14 indicate desiccation tolerance and are candidates for whole-genome sequencing. More time is needed to determine whether a specific strain is truly desiccation tolerant. Furthermore, we conducted an intracellular trehalose assay to test how intracellular trehalose concentrations affect or protect the mutant strains against desiccation stress. S. cerevisiae cell and reagent concentrations from a previously established intracellular trehalose protocol did not yield significant absorbance measurements, so we tested varying cell and reagent concentrations and determined proper concentrations for successful protocol use.

BioSentinel↗
NASA NTRS2016

Pathfinder Technology Demonstrator: GlobalStar Testing and Results

The communications subsystem of a spacecraft is typically a SWaP (size, weight, and power) intensive subsystem in a SWaP constrained environment such as a CubeSat. Use of a satellite-based communication system, such as GlobalStars duplex GSP-1720 radio is a low SWaP potentially game-changing low-cost communication subsystem solution that was evaluated for feasibility for the NASA Pathfinder Technology Demonstrator (PTD) project. The PTD project is a series of 6U CubeSat missions to flight demonstrate and characterize novel small satellite payloads in low Earth orbit. GlobalStar is a low Earth orbit satellite constellation for satellite phone and low-speed data communications, and the GSP-1720 is their single board duplex radio most commonly used in satellite phones and shipment tracking devices. The PTD project tested the GSP-1720 to characterize its viability for flight using NASA GEVS (General Environmental Verification Standard) vibration and thermal vacuum levels, as well as testing the uplink-downlink connectivity, data throughput, and file transfer capabilities. This presentation will present the results of the environmental and capability testing of the GSP-1720 performed at NASA Ames Research Center, as well as the viability for CubeSat use in LEO.

satellite communication↗
NASA NTRS2017

Biosentinel: Improving Desiccation Tolerance of Yeast Biosensors for Deep-Space Missions

BioSentinel is one of 13 secondary payloads to be deployed on Exploration Mission 1 (EM-1) in 2019. We will use the budding yeast Saccharomyces cerevisiae as a biosensor to determine how deep-space radiation affects living organisms and to potentially quantify radiation levels through radiation damage analysis. Radiation can damage DNA through double strand breaks (DSBs), which can normally be repaired by homologous recombination. Two yeast strains will be air-dried and stored in microfluidic cards within the payload: a wild-type control strain and a radiation sensitive rad51 mutant that is deficient in DSB repairs. Throughout the mission, the microfluidic cards will be rehydrated with growth medium and an indicator dye. Growth rates of each strain will be measured through LED detection of the reduction of the indicator dye, which correlates with DNA repair and the amount of radiation damage accumulated. Results from BioSentinel will be compared to analog experiments on the ISS and on Earth. It is well known that desiccation can damage yeast cells and decrease viability over time. We performed a screen for desiccation-tolerant rad51 strains. We selected 20 re-isolates of rad51 and ran a weekly screen for desiccation-tolerant mutants for five weeks. Our data shows that viability decreases over time, confirming previous research findings. Isolates L2, L5 and L14 indicate desiccation tolerance and are candidates for whole-genome sequencing. More time is needed to determine whether a specific strain is truly desiccation tolerant. Furthermore, we conducted an intracellular trehalose assay to test how intracellular trehalose concentrations affect or protect the mutant strains against desiccation stress. S. cerevisiae cell and reagent concentrations from a previously established intracellular trehalose protocol did not yield significant absorbance measurements, so we tested varying cell and reagent concentrations and determined proper concentrations for successful protocol use.

BioSentinel↗
NASA NTRS2016

Mechanotransductive Regulation of Gap-Junction Activity Between MLO-Y4 Osteocyte-Like and MC3T3-E1 Osteoblast-Like Cells in Three-Dimensional Co-Culture

Cell and animal studies conducted onboard the International Space Station and formerly on Shuttle flights have provided groundbreaking data illuminating the deleterious biological response of bone to mechanical unloading. However the intercellular communicative mechanisms associated with the regulation of bone synthesis and bone resorption cells are still largely unknown. Connexin-43 (CX43), a gap junction protein, is hypothesized to play a significant role in osteoblast and osteocyte signaling. The purpose of this investigation was to evaluate within a novel three-dimensional microenvironment how the osteocyte-osteoblast gap-junction expression changes when cultures are exposed to exaggerated mechanical load. MLO-Y4 osteocyte-like cells were cultured on a 3D-Biotek polystyrene insert and placed in direct contact with an MC3T3-E1 pre-osteoblast co-cultured monolayer and exposed to 48 h of mechanical stimulation (pulsatile fluid flow (PFF) or monolayer cyclic stretch (MCS)) then evaluated for viability, proliferation, metabolism, and CX43 expression. Mono-cultured MLO-Y4 and MC3T3-E1 control experiments were conducted under PFF and MCS stimulation to observe how strain application stimuli (PFF cell membrane shear or MCS cell focal adhesionattachment loading) initiates different signaling pathways or downstream regulatory controls. TotalLive cell count, viability and metabolic reduction (Trypan Blue, LIVEDead and Alamar Blue analysis respectively) indicate that mechanical activation of MC3T3-E1 cells inhibits proliferation while maintaining an average 1.04E4 reductioncell metabolic rate, *p0.05 n4. MLO-Y4s in monolayer culture increase in number when exposed to MCS loading but the percent of live cells within the population is low (46.3 total count, *p0.05 n4), these results may indicate an apoptotic signaling cascade. PFF stimulation of the three-dimensional co-cultures elicits a universal increase in CX43 in MLO-Y4 and MC3T3-E1 cells, illustrated by immunohistological observation. Increased CX43 expression is also observed with the three-dimensional co-cultures with MC3T3-E1 MCS stimulation but the increased gap-junction protein presence was limited to the osteoblast-osteocyte interface region. Previously reported PCR evaluation of osteogenic markers further corroborate that the co-cultured populations communicative networks play a role in translating mechanical signals to molecular messaging. These findings suggests an osteocyte-osteoblast gap-junction signaling feedback mechanism may regulate mechanotransduction of apoptosis initiation and transcription of cytokine signaling proteins responsible for stem cell niche recruitment much more directly than previously believed.

Juran, C. M.↗
NASA NTRS2012

SPS-ALPHA: The First Practical Solar Power Satellite via Arbitrarily Large Phased Array

The vision of delivering solar power to Earth from platforms in space has been known for decades. However, early architectures to accomplish this vision were technically complex and unlikely to prove economically viable. Some of the issues with these earlier solar power satellite (SPS) concepts – particularly involving technical feasibility – were addressed by NASA’s space solar power (SSP) studies and technology research in the mid-to-late 1990s. Despite that progress, ten years ago a number of key technical and economic uncertainties remained. A new SPS concept has been proposed that resolves many, if not all, of those uncertainties: “SPSALPHA” (Solar Power Satellite by means of Arbitrarily Large Phased Array). During 2011-2012 the NASA Innovative Advanced Concepts (NIAC) Program supported a Phase 1 “SPS-ALPHA” project, the goal of which was to establish the technical and economic viability of the SPS-ALPHA concept to an early TRL 3 – analytical proof-of-concept – and provide a framework for further study and technology development. The objectives of this project were to: (1) conduct an initial end-to-end systems analysis of the SPS-ALPHA concept in order to determine its technical feasibility; (2) identify and assess in greater detail the key technology challenges inherent in the architecture (including figures of merit for each critical technology area); (3) conduct an initial evaluation of the economic viability of the concept (as a function of key performance parameters); and, (4) define a preliminary roadmap for the further development of the SPS-ALPHA concept.

Mankins, John↗
NASA NTRS2019

Reliability, Safety, and Performance for Two Aerospace Revolutions - UAS/ODM and Commercial Deep Space

Aerospace is in the midst of a renaissance, expanding on both the air and space side into major new commercial areas including unmanned air systems (UAS), on demand mobility (ODM), personal air vehicles (PAV), and commercial deep space. These new areas require, in the initial planning, consideration of new safety, reliability, and in some cases, enabling performance approaches for viability. For example, due to their huge numbers, if current accident rates prevail, UAS/ODM/PAV aircraft could crash at an unacceptable rate, causing life and property damage. Also, if humans in commercial space activities have serious health issues and/or there are unacceptable rocket viability issues/crash rates, these new, major markets (order of 1 trillion dollars per year) could be rapidly curtailed until agreeable and effective changes are instituted, producing additional expense, delay, and reduced revenue. This report addresses such safety and reliability issues and includes: performance enhancement possibilities such as an enabling Air Traffic Control System (ATC), crash proof vehicles, increased range for aero, space debris removal, and human health for space.

Crash Proof↗
NASA NTRSDate not supplied

Space Shuttle Program Dual Docked Operations

Due to the ever-increasing visiting vehicle traffic to and from the International Space Station (ISS) during mated Orbiter/ISS missions, it became apparent to both the ISS Program (ISSP) and the Space Shuttle program (SSP) that there would arise occasions where a visiting vehicle docking and/or undocking could overlap when the Space Shuttle Orbiter was docked to the ISS, referred to as Dual Docked Operations (DDO). The primary concerns were that plumes from the visiting vehicles would cause contamination, corrosion, and erosion to Space Shuttle surfaces and present a toxicity hazard to ground personnel when processing the Space Shuttle after landing. This potential conflict provided the genesis for evaluating risk mitigations to gain maximum flexibility for managing potential visiting vehicle traffic to and from the ISS and to maximize launch and landing opportunities for all visiting vehicles. The overarching goal of this effort was to assess the viability and readiness of the SSP to protect for DDO on any given mission. To assess the viability of performing DDO, the ISSP delivered plume particle fluence (i.e., number of particles per unit area) and contamination deposition based on the visiting vehicle’s proximity operations to the SSP for evaluation. This paper summarizes the concept definition, studies, and analysis results generated by the SSP, ISSP, and Mission Operations Directorate (MOD) for implementing DDO involving the Orbiter and Russian vehicles (RVs). Discussion and results presented herein represent a generic assessment of the SSP and ISSP integrated safety and technical evaluations of DDO involving the Orbiter and RVs. Mission-specific analysis and operational impacts were conducted flight-by-flight as they arose.

Launch vehicles↗
NASA NTRS2023

Market Analysis of the Subsonic Single Aft Engine (SUSAN) Transport Aircraft Concept

This paper presents a detailed market analysis of the U.S. domestic aviation market in support of the NASA subsonic single aft engine (SUSAN) regional aircraft concept. The current scoping of the SUSAN concept is intended to compete in the medium to large (160-180 seats) narrow body market, with range capabilities of up to 2,500 nautical miles, and expected fuel burn reduction up to 40% relative to conventional 2 engine aircraft. Recent historical trends suggest growth in aviation passenger demand will continue to be met by the narrow body fleet of aircraft; however, a comprehensive review is required to understand how these current fleet trends could evolve in the future. Moreover, estimating and forecasting the potential market size is the critical first step when developing a new aircraft concept to determine commercial viability. To assist in the trade space exploration of the SUSAN concept, a generalized traffic and fleet forecast of the U.S. aviation market is conducted. Using publicly available aviation data from the U.S. Bureau of Transportation Statistics and passenger demand forecasts from FAA, a multinomial logit model is estimated to predict the composition of the future fleet by aircraft size. These fleet forecasts are then used as inputs for a fleet evolution model to provide required operational forecasts at the aircraft specific level. Forecast scenarios with and without the SUSAN concept are compared, and a breakeven analysis is performed to evaluate the commercial viability of the SUSAN aircraft from an operating cost perspective. Results from the multinomial logit fleet forecast indicate the narrow body size category of 150+ seats dominating the market, comprising 87% of the future revenue passenger miles market share in 2050 (up from 60% in 2019). Forecast scenarios with the SUSAN concept see a maximum cumulative decline in fuel cost of 20% by 2050, while a breakeven analysis shows competitive advantage of the SUSAN aircraft, due to expected fuel burn reduction, at moderate levels of increased maintenance and capital costs.

Jacob M. Wishart↗
NASA NTRS2022

Lunar Communications Services with Emphasis on Commercialization

In mid-2020 Lockheed Marin Space (LM) and NASA’s Jet Propulsion Laboratory (JPL) formed a working group to study and address the need for a lunar communications network to service a growing lunar economy, beginning with the initial crewed and robotic mission needs in 2024. Multiple commercial relay architectures were studied and evaluated for platform requirements, communications capabilities, and commercial business viability in a growing ecosystem. The evolution of communications needs around the Moon were mapped to infrastructure build out. Trade studies were performed to evaluate reliability and lifetime requirements, and the viability of various data protocols. Mission concepts and operations plans were developed for both initial mission support and future autonomous network support. This work has led to Lockheed Martin and JPL to evaluate a single dedicated relay satellite in a frozen lunar orbit, with simultaneous coverage of the far-side and south pole landing sites for over 10 hours per day as a first step towards a comprehensive solution to lunar connectivity. This first satellite is compatible with an ESPA Grande launch volume and would be capable of launching on a rideshare into a variety of orbits and trajectories. The satellite is equipped with both high- and low-rate communications relay payloads with software-defined radios and a delay/disruption tolerant networking protocol. The system is designed to service initial and future mission needs in a commercialized manner, enabling a new class of missions to the Moon. The relay satellite leverages prior spacecraft platform work with on-orbit heritage in a lunar environment expected by 2023.

Davarian, Faramaz↗
NASA NTRSDate not supplied

Structural Requirements and Scaling Analysis of a Fluidic Mirror Space Telescope Support Structure

The NASA FLUTE project proposes large-scale (50m) fluidic telescopes for astronomy applications. To continue to explore the universe, astronomers require larger and larger telescope apertures. The highest priority astrophysics targets such as exoplanets and early galaxies are extremely faint, motivating larger telescope apertures. However, mission costs depend on aperture diameter, and scaling apertures beyond 10-m apertures faces economic and technological viability challenges. An unsegmented primary mirror made in space via fluidic microgravity shaping would provide a scalable and cost-effective method to scale apertures to 50-m scale while achieving sub-nanometer (root mean square) surface quality. Such microgravity fluidic shaping has been demonstrated in laboratory neutral buoyance environments, parabolic microgravity experiments, as well aboard the International Space Station. One of the main components of a fluidic observatory is the mirror frame. The frame must provide a stable bounding circular ring which the edges of the fluid mirror surface can wet. The frame can optionally provide a ‘floor’ surface on the interior of the ring to provide additional fluid support and reduce required fluid volume. In this work, we evaluate several classes of structural frame architectures potentially suitable for a fluidic telescope support structure. We start by estimating stability requirements, orbital, station keeping, and slew loads based on a notional CONOPS. The scaling of overall fluid mass required for each architecture is evaluated. Preliminary results elucidate the importance of a support floor for overall mission viability above 10-m diameter. We then investigate the scaling of a tetrahedral truss frame support structure. We show that segmented solid shell support surfaces can provide sufficient stability at modest mass fractions. We estimate that the total fluid and frame mass for a 50-m telescope could be on the order of 15,000 kg. Finally, implementation considerations are discussed, including deployment/assembly methodologies. The results of this study establish feasibility of a large-scale fluidic telescope and will guide further architecture development and detailed structural design.

Christine Gregg↗
NASA NTRS2024

Evaluation of Cleaning Methods for Dust Mitigation of Lunar Docking and Hatch Seals

Low-leakage seals are an enabling technology for future lunar surface systems, and they must be kept clean to ensure that crews have sufficient breathable air for extended lunar surface missions. Previous testing on representative state-of-the-art seals showed that while seals can tolerate some level of lunar simulant dust contamination, dust can degrade seal performance to a point at which seal leak rates exceed maximum allowable values. The ability to clean simulant from seals had not been thoroughly studied, and the effectiveness and potential complications of using different cleaning methods for lunar seals were not well understood. To address this knowledge gap, researchers at the NASA Glenn Research Center executed a multiphase study to better understand and mitigate seal dust-exposure risks. The focus of this report is on the second phase of that study, in which tests were conducted to evaluate the viability of seal cleaning methods for use in the lunar environment. Multiple methods for cleaning seals contaminated with lunar simulant were assessed for cleaning effectiveness and viability for use in the lunar environment. Leak tests were performed on seals after they were cleaned to determine whether leak rates could be brought back to acceptable levels, and cleaned seals were examined to determine whether their surfaces had been damaged by the cleaning processes. This phase of the study also investigated changes in the seals’ adhesion forces as a result of contamination and cleaning.

Dust Mitigation↗
NASA NTRSDate not supplied

A Heritage BioSensor for Lunar Biology Experiments

Introduction: Automated biological experiments on small spacecraft missions have gained prominence over the past decade due to their simplicity, accessibility, and small mass, volume, and power needs. Most recently, the BioSensor microfluidic CubeSat payload aboard BioSentinel used an automated microfluidic cell culture system to study the effects of environmental stressors like deep space radiation and microgravity on yeast growth and metabolism. BioSentinel’s successor, the Lunar Explorer Instrument for space biology Applications (LEIA), will study the effects of lunar gravity and radiation using an improved version of the BioSensor microfluidic platform. The BioSensor payload has great adaptability to host a diverse range of biological experiments with single- and multi-celled organisms in both crewed and uncrewed missions, making it a compelling candidate for future space biology studies in a lunar surface environment. BioSensor Instrumentation on BioSentinel: The first spaceflight mission with the BioSensor, BioSentinel’s biology experiments occurred at three locations -- deep space, ISS and ground. The payload contained 18 microfluidic cards, each featuring 16 growth wells (a total of 288 growth wells). Each well was loaded before launch with desiccated yeast. In space, liquid culture medium (nutrients) was automatically introduced to batches of wells at a time to initiate a series of biology experiments. Temperature was maintained by thin film heaters on both sides of each card. Each well was equipped with three LEDs emitting at 570 nm, 630 nm, and 850 nm, paired with photodetectors to measure cell concentration and the alamarBlue (metabolic indicator dye) color transition from blue to pink. Phenotypic parameters like cell viability, metabolic rate, and generation time can be derived from these measurements. The sequence and timing of fluid fills, optical measurements, and thermal control were stored onboard, but could be updated asynchronously via ground communication. LEIA: LEIA is slated for launch no earlier than 2026 on a CLPS lander. BioSentinel’s BioSensor has been modified for use in LEIA. These improvements include: (a) storage for multiple culture medium types, (b) additional LED color (465 nm) for a new biological assay for antioxidant (carotenoid) production, (c) housing modifications for later biology load before launch, (d) improved isolation between electronic and fluidic components, and (e) improved humidity control for prolonged organism viability in case of post-load launch delay. Future Prospects: The consistent and successful demonstration of complex fluidics platforms alongside reliable instrument operations in a space environment is poised to create strong momentum for BioSensor-based biological experiment payloads. Planned future developments with the BioSensor include extending compatibility to a broader range of organisms and assays. Preliminary work has already demonstrated successful growth of Arabidopsis seedlings in fluidic cards. With a few modifications to the optical assembly, the setup could easily measure photosynthetic traits in plants and cyanobacteria. The addition of fluorescence measurements and generation of novel luminescent assays will elevate BioSensor’s functionality further. Beyond the BioSensor’s potential uses on free-flyer missions, ISS and Gateway, and CLPS landers, deploying the BioSensor to the lunar surface or in an artificial habitat on crewed missions could enable pioneering research on both how life responds to lunar conditions and future bioproduction capabilities making the BioSensor an indispensable tool for future space biology research.

Chinmayee Govinda Raj↗
NASA NTRSDate not supplied

Using Global Market Demand Analysis to Guide Conceptual Design of Low-Boom Supersonic Transports

This paper uses a mathematical framework to identify the interdependencies of key variables in supersonic transportation demand analysis. The existing quantitative models for supersonic transportation demand analysis are compared for consistency in modeling the interdependencies. Unlike other quantitative models, the Low-Boom Systems Analysis Model (LBSAM2) can propagate important design characteristics of a supersonic transport aircraft concept to the level of economic metrics (such as the number of future supersonic passengers), with a consistent coupling of the market demand analysis, detailed mission analysis, and low-boom constraint. This allows the use of the detailed demand analysis results from LBSAM2 to maximize the economic viability of a supersonic transport aircraft by finding favorable system-level trades between weight, range, fuel burn, and assumed sonic boom ground noise limit for supersonic overland flight. In this paper, LBSAM2 is integrated with conceptual low-boom design to improve the economic viability of low-boom supersonic transport aircraft. A brief discussion of uncertainties in the LBSAM2 analysis is also included, focusing on their impacts on the relative economic advantages between low-boom concepts.

Consistent coupling of disciplinary analyses↗