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At least 343 records · Page 19

Culture-Independent Microbial Air Profiling using a Spaceflight-Compatible Nanopore Sequencing Method

Microbial monitoring of spacecraft air is critical toward assessing the efficacy of microbial controls within the environmental control and life support systems to protect both the crew and the vehicle environment. Additionally, understanding of the atmospheric microbial profiles will be essential toward Mars-forward planetary protection planning. Currently, onboard the International Space Station (ISS), the air is monitored on a quarterly basis using an impaction air sampler. With this method, bacterial and fungal cells and spores are pulled onto plates containing growth medium. Following five days of ambient incubation onboard the ISS, the crew compares the growth to density charts and provides the approximations to the ground. Upon return of the culture plates to the Johnson Space Center’s Microbiology Laboratory, the isolates present are identified for crew health risk assessments. As NASA moves beyond the low-Earth orbit of ISS, sample return will be impractical. As such, a near real-time monitoring capability for the assessment of the spacecraft atmosphere is necessary. Significant strides have been made in recent years to utilize a molecular-based method for microbial profiling of ISS surfaces. The developed method is not dependent on microbial culture, thus removing the associated risk to the crew from high microbial levels, the bias toward detecting only the culturable organisms, and eliminates the need for sample return. The work described here details the evaluation of three different air sampling platforms whose product is amenable to downstream molecular processing and nanopore sequencing. The three samplers were compared in terms of mass and power requirements, ease-of -use, and the resulting data. For the two highest ranking samplers, a basic concept of operations was developed to transfer the sample into the already established preparation and sequencing process. Using these concepts of operations, an in-depth comparison of the molecular data generated was compared to the historical culture-based method. Data from both methods detailed similar microbial profiles, while the molecular method detailed microbial identifications that could not be identified through the culture-based method. The developed method will enable the generation of near real-time microbial profiles of the spacecraft atmosphere.

Brandon J Dunbar↗

Design of a Jettison System For Space Transit Vehicles

Many options to re-use waste are currently being developed by NASA. These include combustion, compaction, torrefaction, and converting waste materials to an easily stored base polymer for future use. Human exploration missions require large amounts of supplies such as food, clothing and spare parts. A many-month journey to Mars will result in the generation of a substantial amount of problematic waste products. It is thought that this waste must be discarded to enable a Mars transit mission. The most cost-effective, reliable, and safest method to address this problem may be to simply jettison these materials from the spacecraft. The ability to jettison requires a multi-component integrated system design. Major components include a launcher, airlocks, trash bags, and tracking system. Depending upon mission requirements, a jettison dedicated airlock may be necessary. In other cases, the crew airlock might be all that’s needed. Examples of what to jettison include food waste, fecal containers, broken equipment, foam, expendables, hygiene products, health items, wipes, and clothing. The most important design factor is always crew safety. Other features to consider are loss of air due to the inefficiencies of vacuum pumps in the airlock, outgassing from the bags, and liquids condensing on the airlock that result in unwanted coatings. The launching system design must consider many different features: while in planetary orbit, a minimum exit velocity is necessary so the jettisoned material will not complete an orbit only to return and strike the ship. Also, for planetary protection issues, enabling waste to be sent to distant non-critical locations is highly desirable. When the space craft is in planetary transit to say, Mars, the jettison exit velocity should be low if the material is not released along the direction of travel. This is to minimize trajectory perturbations due to jettison-imposed torque on the spacecraft. Design considerations for the launcher include exit velocity, power consumption, volume and mass of material jettisoned, and frequency of jettison. Finally, the launcher must be able to handle many different types and shapes of waste products. This paper will discuss these design issues and give guidance to a pathway forward.

Steve Sepka↗

Effects of Desiccation and Freezing on Microbial Ionizing Radiation Survivability: Considerations for Mars Sample Return

Increasingly, national space agencies are expanding their goals to include Mars exploration with sample return. To better protect Earth and its biosphere from potential extraterrestrial sources of contamination, as set forth in the Outer Space Treaty of 1967, international efforts to develop planetary protection measures strive to understand the danger of cross-contamination processes in Mars sample return missions. We aim to better understand the impact of the martian surface on microbial dormancy and survivability. Radiation resistance of microbes is a key parameter in considering survivability of microbes over geologic times on the frigid, arid surface of Mars that is bombarded by solar and galactic cosmic radiation. We tested the influence of desiccation and freezing on the ionizing radiation survival of six model microorganisms: vegetative cells of two bacteria (Deinococcus radiodurans, Escherichia coli) and a strain of budding yeast (Saccharomyces cerevisiae); and vegetative cells and endospores of three Bacillus bacteria (B. subtilis, B. megaterium, B. thuringiensis). Desiccation and freezing greatly increased radiation survival of vegetative polyploid microorganisms when applied separately, and when combined, desiccation and freezing increased radiation survival even more so. Thus, the radiation survival threshold of polyploid D. radiodurans cells can be extended from the already high value of 25 kGy in liquid culture to an astonishing 140 kGy when the cells are both desiccated and frozen. However, such synergistic radioprotective effects of desiccation and freezing were not observed in monogenomic or digenomic Bacillus cells and endospores, which are generally sterilized by 12 kGy. This difference is associated with a critical requirement for survivability under radiation, that is, repair of genome damage caused by radiation. Deinococcus radiodurans and S. cerevisiae accumulate similarly high levels of the Mn antioxidants that are required for extreme radiation resistance, as do endospores, though they greatly exceed spores in radioresistance because they contain multiple identical genome copies, which in D. radiodurans are joined by persistent Holliday junctions. We estimate ionizing radiation survival limits of polyploid DNA-based life-forms to be hundreds of millions of years of background radiation while buried in the martian subsurface. Our findings imply that forward contamination of Mars will essentially be permanent, and backward contamination is a possibility if life ever existed on Mars.

59 BASIC BIOLOGICAL SCIENCES↗

Atmospheric Escape Processes and Planetary Atmospheric Evolution: From Misconceptions to Challenges

The recent discoveries of telluric exoplanets in the habitable zone of different stars have led to questioning the nature of their atmosphere, which is required to determine their habitability. Atmospheric escape is one of the challenging problems to be solved: simply adapting what is currently observed in the solar system is doomed to fail due to the large variations in the conditions encountered around other stars. A better strategy is to evaluate the different processes that shaped planetary atmosphere and to evaluate their importance depending upon the stellar conditions. We reviewed the different escape mechanisms and their magnitude in function of different conditions [Gronoff et al. 2020]. This led us to discuss the importance of a magnetic field in protecting an atmosphere. The importance of the thermal escape, of polar wind, and of the transport of plasma within the magnetosphere are typically forgotten when claiming that magnetic fields are protecting planetary atmospheres and leading to their habitability. Overall, the habitability of a planet should not be claimed only on by its location in the habitable zone but also after careful analysis of the interaction between its atmosphere and its parent star . Gronoff, G., Arras, P., Baraka, S., Bell, J. M., Cessateur, G., Cohen, O., et al. ( 2020). Atmospheric Escape Processes and Planetary Atmospheric Evolution. Journal of Geophysical Research: Space Physics, 125, e2019JA027639. https://doi.org/10.1029/2019JA027639

G Gronoff↗

Thermal Protection Materials for Reentry and Planetary Applications

Thermal protection materials and systems (TPS) are used to protect spacecraft during reentry into Earth's atmosphere or entry into planetary atmospheres. As such, these materials are subject to severe environments with high heat fluxes and rapid heating. Catalytic effects can increase the temperatures substantially. These materials are also subject to impact damage from micrometeorites or other debris during ascent, orbit, and descent, and thus must be able to withstand damage and function following damage. Thermal protection materials and coatings used in reusable launch vehicles will be reviewed, including the needs and directions for new materials to enable new missions that require faster turnaround and much greater reusability. The role of ablative materials for use in high heat flux environments, especially for non-reusable applications and upcoming planetary missions, will be discussed. New thermal protection system materials may enable the use of sharp nose caps and leading edges on future reusable space transportation vehicles. Vehicles employing this new technology would have significant increases in maneuverability and out-of-orbit cross range compared to current vehicles, leading to increased mission safety in the event of the need to abort during ascent or from orbit. Ultrahigh temperature ceramics, a family of materials based on HfB2and ZrB2 with SiC, will be discussed. The development, mechanical and thermal properties, and uses of these materials will be reviewed.

Johnson, Sylvia M.↗

Operational Lessons Learned from NASA Analog Missions

National Aeronautics and Space Administration s (NASA) efforts in human space flight are currently focused on the Space Shuttle and International Space Station (ISS) programs, with efforts beginning on the future exploration opportunities. Both the Space Shuttle and ISS programs are important to the development of a capability for human exploration beyond Low Earth Orbit (LEO). The ISS provides extensive research capabilities to determine how the human body reacts to long duration stays in space. Also, the ISS and Shuttle can serve as a limited testbed for equipment or entire systems that may be used on missions to the Moon, Mars, or to a near-Earth asteroid. It has been nearly 35 years since the Apollo astronauts visited the Moon. Future space explorers will have to re-learn how to work and live on planetary surfaces, and how to do that for extended periods of time. Exploration crews will perform a wide assortment of scientific tasks, including material sampling and emplacement of automated instruments. Surface mission operations include the activities of the crew living and working, mission support from the Earth, and the operation of robotic and other remotely commanded equipment on the surface and in planetary orbit. Other surface activities will include the following: exploring areas surrounding a habitat; using rovers to collect rock and soil samples; setting up experiments on the surface to monitor the radiation environment and any seismic or thermal activity; and conducting scientific analyses and experiments inside a habitat laboratory. Of course, the astronauts will also have to spend some of their surface time "doing chores" and maintaining their habitat and other systems. In preparation for future planetary exploration, NASA must design the answers to many operational questions. What will the astronauts do on the surface? How will they accomplish this? What tools will they require for their tasks? How will robots and astronauts work together? What vehicle and system capabilities are required to support the activities? How will the crew and the Earth-based mission control team interact? During the initial phases of manned planetary exploration, one challenge in particular is virtually the same as during the Apollo program: How can scientific return be maximized during a relatively short surface mission? Today, NASA is investigating solutions to these challenges by conducting analog missions. These Earth-based missions possess characteristics that are analogous to missions on the Moon or Mars. These missions are excellent for testing operational concepts, and the design, configuration, and functionality of spacesuits, robots, rovers, and habitats. Analog mission crews test specific techniques and procedures for surface field geology, biological sample collection, and planetary protection. The process of actually working an analog mission reveals a myriad of small details, which either contribute to or impede efficient operations, many of which would never have been thought about otherwise. It also helps to define the suite of tools, containers, and other small equipment that surface explorers will use. This paper focuses on how analog missions have addressed selected operational considerations for future planetary missions.

Arnold, Larissa S.↗

Thermal Protection System Materials for Sample Return Missions

This white paper from the Thermal Protection System (TPS) community to the NRC Decadal Survey Sub-Panels provides an overview of TPS materials needed for future Sample Return (SR) missions. We consider the capability of heritage TPS material used by recent SR missions and identify appropriate materials for future SR missions. A prime conclusion is that the current TPS materials, if properly maintained, offer good low-density solutions for lower velocity (<13.5 km/s) sample return missions without planetary protection back-protection concerns. Furthermore, missions that have a larger capsule, a higher entry speed (>13.5 km/s), or back-protection concerns will leverage recently developed mid-density TPS materials. To maintain NASA’s Sample Return capabilities in the coming decade, we recommend that NASA continue to invest in sustainment of relevant TPS materials, as well as ground-test facilities, predictive entry modeling, and flight instrumentation.

mission design↗

MSR Curation Technology Development – Developing Protocols for Pre-Basic Characterization Measurements

The joint NASA/ESA Mars Sample Return (MSR) campaign could deliver to Earth the first pristine Mar-tian rock and regolith samples, collected by the Mars2020 science mission, Perseverance. In preparation for their arrival, the NASA/ESA Joint Curation Office (JCO) within the Sample Receiving Project (SRP) is developing curation technology needed to perform initial characterization of the samples while isolating samples from the Earth’s biosphere and protecting the samples from terrestrial contamination. (RSTA; Fig. 1). The Mars Sample Return Planning Group 2 (MSPG2) identified methods for achieving an array of potential science objectives for MSR outlined by the International MSR Objectives and Samples Team (iMOST) [1]. One category of methods, called Pre-Basic Characterization, recommends potential analyses that would happen before the Returnable Sample Tube Assemblies (RSTA; Figure 1) are opened: 1) Magnetometer & Magnetic Susceptibility; 2) X-ray Computed Tomography (XCT) Scanner [2]. To be in compliance with planetary protection requirements, and to limit the amount of time sample tubes are out-side of an isolated contamination-controlled environment, secondary containment is necessary. A Sample Tube Isolation Container (STIC) and Secondary Outer Containment Case (SOCC) is currently being developed at NASA JPL under the direction of the SRP JCO.

V Tu↗

Enigma Version 12

Enigma Version 12 software combines model building, animation, and engineering visualization into one concise software package. Enigma employs a versatile user interface to allow average users access to even the most complex pieces of the application. Using Enigma eliminates the need to buy and learn several software packages to create an engineering visualization. Models can be created and/or modified within Enigma down to the polygon level. Textures and materials can be applied for additional realism. Within Enigma, these models can be combined to create systems of models that have a hierarchical relationship to one another, such as a robotic arm. Then these systems can be animated within the program or controlled by an external application programming interface (API). In addition, Enigma provides the ability to use plug-ins. Plugins allow the user to create custom code for a specific application and access the Enigma model and system data, but still use the Enigma drawing functionality. CAD files can be imported into Enigma and combined to create systems of computer graphics models that can be manipulated with constraints. An API is available so that an engineer can write a simulation and drive the computer graphics models with no knowledge of computer graphics. An animation editor allows an engineer to set up sequences of animations generated by simulations or by conceptual trajectories in order to record these to highquality media for presentation. Enigma Version 12 Lyndon B. Johnson Space Center, Houston, Texas 28 NASA Tech Briefs, September 2013 Planetary Protection Bioburden Analysis Program NASA's Jet Propulsion Laboratory, Pasadena, California This program is a Microsoft Access program that performed statistical analysis of the colony counts from assays performed on the Mars Science Laboratory (MSL) spacecraft to determine the bioburden density, 3-sigma biodensity, and the total bioburdens required for the MSL prelaunch reports. It also contains numerous tools that report the data in various ways to simplify the reports required. The program performs all the calculations directly in the MS Access program. Prior to this development, the data was exported to large Excel files that had to be cut and pasted to provide the desired results. The program contains a main menu and a number of submenus. Analyses can be performed by using either all the assays, or only the accountable assays that will be used in the final analysis. There are three options on the first menu: either calculate using (1) the old MER (Mars Exploration Rover) statistics, (2) the MSL statistics for all the assays, or This software implements penetration limit equations for common micrometeoroid and orbital debris (MMOD) shield configurations, windows, and thermal protection systems. Allowable MMOD risk is formulated in terms of the probability of penetration (PNP) of the spacecraft pressure hull. For calculating the risk, spacecraft geometry models, mission profiles, debris environment models, and penetration limit equations for installed shielding configurations are required. Risk assessment software such as NASA's BUMPERII is used to calculate mission PNP; however, they are unsuitable for use in shield design and preliminary analysis studies. The software defines a single equation for the design and performance evaluation of common MMOD shielding configurations, windows, and thermal protection systems, along with a description of their validity range and guidelines for their application. Recommendations are based on preliminary reviews of fundamental assumptions, and accuracy in predicting experimental impact test results. The software is programmed in Visual Basic for Applications for installation as a simple add-in for Microsoft Excel. The user is directed to a graphical user interface (GUI) that requires user inputs and provides solutions directly in Microsoft Excel workbooks. This work was done by Shannon Ryan of the USRA Lunar and Planetary Institute for Johnson Space Center. Further information is contained in a TSP (see page 1). MSC- 24582-1 Micrometeoroid and Orbital Debris (MMOD) Shield Ballistic Limit Analysis Program Lyndon B. Johnson Space Center, Houston, Texas Commercially, because it is so generic, Enigma can be used for almost any project that requires engineering visualization, model building, or animation. Models in Enigma can be exported to many other formats for use in other applications as well. Educationally, Enigma is being used to allow university students to visualize robotic algorithms in a simulation mode before using them with actual hardware.

Shores, David↗

Facility Concepts for Mars Returned Sample Handling

Samples returned from Mars must be held in quarantine until their biological safety has been determined. A significant challenge, unique to NASA's needs, is how to contain the samples (to protect the blaspheme) while simultaneously protecting their pristine nature. This paper presents a comparative analysis of several quarantine facility concepts for handling and analyzing these samples. The considerations in this design analysis include: modes of manipulation; capability for destructive as well as non-destructive testing; avoidance of cross-contamination; linear versus recursive processing; and sample storage and retrieval within a closed system. The ability to rigorously contain biologically hazardous materials has been amply demonstrated by facilities that meet the specifications of the Center for Disease Control Biosafety Level 4. The newly defined Planetary Protection Level Alpha must provide comparable containment while assuring that the samples remain pristine; the latter requirement is based on the need to avoid compromising science analyses by instrumentation of the highest possible sensitivity (among other things this will assure that there is no false positive detection of organisms or organic molecules - a situation that would delay or prevent the release of the samples from quarantine). Protection of the samples against contamination by terrestrial organisms and organic molecules makes a considerable impact upon the sample handling facility. The use of glove boxes appears to be impractical because of their tendency to leak and to surges. As a result, a returned sample quarantine facility must consider the use of automation and remote manipulation to carry out the various functions of sample handling and transfer within the system. The problem of maintaining sensitive and bulky instrumentation under the constraints of simultaneous sample containment and contamination protection also places demands on the architectural configuration of the facility that houses it.

Cohen, Marc M.↗

Microbial Methods for Testing a Novel Spacecraft Bioburden Reduction Method

In order to circumvent the contamination of microorganisms in both extraterrestrial and terrestrial environments, spacecraft hardware must often be sterilized. Current sterilization practices are both time-consuming and expensive. Techniques such as dry heat sterilization and scattered/localized electric field enhancements are incompatible with a variety of hardware materials as well as complex surface shapes. Our group is testing a novel femtosecond pulsed laser for the rapid sterilization of spacecraft hardware using high photon fluxes, which is both an efficient and effective technique to ensure a reduced microbial burden aboard spacecraft. This method is likely to be effective because microorganisms have not encountered high photon fluxes in their evolutionary history and thus contain no protective mechanisms against them. To assess the effectiveness of pulsed laser sterilization, we used a combination of scanning electron microscopy and elemental analysis for the examination of damaged spores on metal coupons, as well as microbiology methods for spore assays. The surfaces of metal coupons inoculated with Bacillus subtilis were illuminated with femtosecond pulses at various wavelengths below the damage threshold for the aluminum. After laser treatment, metal coupon samples were sonicated to dislodge spores, serially diluted, and finally plated and incubated. Results show that sterilization efficiency is favorable in some conditions. The NASA standards for spore assays are a preliminary demonstration in establishing the trend in ability to kill spores. The results show potential for the dual pulse illumination method as a sterilization technique for planetary protection applications.

Maha S Ulhaq↗

The Integration of Life Sciences in Space: Astrobiology and Space Biology Virtual Workshops Report

A series of virtual workshops was held during June 2020 to seek ways to integrate the efforts of the astrobiology and space biology research communities under a broad umbrella of space life sciences. The overall goal was to help inspire creativity that will guide us towards new synergistic ideas complementing these existing disciplines that are of such importance to NASA. Workshop participants aspired to: (1) Exploit synergies across the biological sciences at NASA, (2) Foster research, enabling technology, and mission concepts that support commonalities in space biology, astrobiology, synthetic biology, planetary protection, and relevant human health, performance, and habitation concerns, (3) Envision the development of an “Arc of Biology in Space” to encompass this multi-faceted joint research community. The focused objective of the workshop series was to explore and demonstrate how the integration of astrobiology and space biology could be achieved, identify strengths and weaknesses in the current state of the art, and recognize where our greatest challenges lay. Specifically, we seek to: (1) Establish a scientific framework for an integrated life sciences effort, (2) Pioneer discovery by creating unique opportunities in the fundamental biological sciences, (3) Explore novel combinations of existing technologies across the relevant disciplines, (4) Invent new technologies and applications in space life sciences, and (5) Creatively increase access to spaceflight, emerging and novel technologies, Earth analogs, and simulated natural and spaceflight environments. The community aims for a broad arc of biological competence in the context of space and planetary science, spaceflight, and habitation. We will present dominant themes and innovative ideas that resulted from this interchange of relevant communities.

astrobiology↗

The Integration of Life Sciences in Space: Astrobiology and Space Biology Virtual Workshops Report

A series of virtual workshops was held during June 2020 to seek ways to integrate the efforts of the astrobiology and space biology research communities under a broad umbrella of space life sciences. The overall goal was to help inspire creativity that will guide us towards new synergistic ideas complementing these existing disciplines that are of such importance to NASA. Workshop participants aspired to: (1) Exploit synergies across the biological sciences at NASA, (2) Foster research, enabling technology, and mission concepts that support commonalities in space biology, astrobiology, synthetic biology, planetary protection, and relevant human health, performance, and habitation concerns, (3) Envision the development of an “Arc of Biology in Space” to encompass this multi-faceted joint research community. The focused objective of the workshop series was to explore and demonstrate how the integration of astrobiology and space biology could be achieved, identify strengths and weaknesses in the current state of the art, and recognize where our greatest challenges lay. Specifically, we seek to: (1) Establish a scientific framework for an integrated life sciences effort, (2) Pioneer discovery by creating unique opportunities in the fundamental biological sciences, (3) Explore novel combinations of existing technologies across the relevant disciplines, (4) Invent new technologies and applications in space life sciences, and (5) Creatively increase access to spaceflight, emerging and novel technologies, Earth analogs, and simulated natural and spaceflight environments. The community aims for a broad arc of biological competence in the context of space and planetary science, spaceflight, and habitation. We will present dominant themes and innovative ideas that resulted from this interchange of relevant communities.

astrobiology↗

Characterization of Spacesuit Associated Microbial Communities and their Implications for NASA Missions

Crewed National Aeronautics and Space Administration (NASA) missions to other solar system bodies are currently being planned. One high-profile scientific focus during such expeditions would be life detection, specifically the discovery of past or present microbial life, if they exist. However, both humans and associated objects typically carry a high microbial burden. Thus, it is essential to distinguish between microbes brought with the expedition and those present on the exploring planets. Modern spacesuits are unique, customized spacecraft which provide protection, mobility and life support to crew during spacewalks, yet they vent continuously, and the mobility of microbes through spacesuits has not been studied. To evaluate the microbial colonization of spacesuits, NASA used an Extravehicular Activity swab kit to examine viable microbial populations of 48 samples from spacesuits using both traditional microbiological methods and molecular sequencing methods. The cultivable microbial population ranged from below the detection limit to 9 x 102 colony forming units per 25 cm2 of sample and also significantly varied by the location. The cultivable microbial diversity was dominated by members of Bacillus, Arthrobacter, and Ascomycota. However, 16S rRNA-based viable bacterial burden ranged from 105 to 106 copies per 25 cm2 of sample. Shotgun metagenome sequencing revealed the presence of a diverse microbial population on the spacesuit surfaces, including Curtobacterium and Methylobacterium from across all sets of spacesuits in high abundance. Among bacterial species identified, higher abundance of Cutibacterium acnes, Methylobacterium oryzae, and M. phyllosphaerae reads were documented. The results of this study provide evidence that identical microbial strains may live on the wrist joint, inner gauntlet, and outer gauntlet of spacesuits. This raises the possibility, but does not confirm, that viable microbes are able to migrate between the interior and exterior of spacesuits. Overall, these data provide the first estimate of microbial dispersion from internal to external spacesuit surfaces, which will help future mission planners develop effective planetary protection strategies.

spacesuit↗

Quo vadis? Microbial profiling revealed strong effects of cleanroom maintenance and routes of contamination in indoor environments

Space agencies maintain highly controlled cleanrooms to ensure the demands of planetary protection. To study potential effects of microbiome control, we analyzed microbial communities in two particulate-controlled cleanrooms (ISO 5 and ISO 8) and two vicinal uncontrolled areas (office, changing room) by cultivation and 16S rRNA gene amplicon analysis (cloning, pyrotagsequencing, and PhyloChip G3 analysis). Maintenance procedures affected the microbiome on total abundance and microbial community structure concerning richness, diversity and relative abundance of certain taxa. Cleanroom areas were found to be mainly predominated by potentially human-associated bacteria; archaeal signatures were detected in every area. Results indicate that microorganisms were mainly spread from the changing room (68%) into the cleanrooms, potentially carried along with human activity. The numbers of colony forming units were reduced by up to ~400 fold from the uncontrolled areas towards the ISO 5 cleanroom, accompanied with a reduction of the living portion of microorganisms from 45% (changing area) to 1% of total 16S rRNA gene signatures as revealed via propidium monoazide treatment of the samples. Our results demonstrate the strong effects of cleanroom maintenance on microbial communities in indoor environments and can be used to improve the design and operation of biologically controlled cleanrooms.

42 ENGINEERING↗

Radiation Testing at Sandia National Laboratories: Sandia – JPL Collaboration for Europa Lander

Sandia National Laboratories (SNL) is assisting Jet Propulsion Laboratory in undertaking feasibility studies and performance assessments for the Planetary Protection aspect of the Europa Lander mission. The specific areas of interest for this project are described by task number. This white paper presents the evaluation results for Task 2, Radiation Testing, which was stated as follows: Survey SNL facilities and capabilities for simulating the Europan radiation environment and assess suitability for: A. Testing batteries, electronics, and other component and subsystems B. Exposing biological organisms to assess their survivability metrics. The radiation environment the Europa Lander will encounter on route and in orbit upon arrival at its destination consists primarily of charged particles, energetic protons and electrons with the energies up to 1 GeV. The charged particle environments can be simulated using the accelerators at the Ion Beam Laboratory. The Gamma Irradiation Facility and its annex, the Low Dose Rate Irradiation Facility, offer irradiations using Co-60 gamma sources (1.17 and 1.33 MeV), as well as Cs-137 gamma (0.661 MeV) AmBe neutron (0-10 MeV) sources.

07 ISOTOPE AND RADIATION SOURCES↗

Radioisotope Power Systems: Considerations for use of Dynamic versus Static Conversion Technologies for NASA Missions

For more than five decades, Radioisotope Power Systems (RPS) have played a critical role in the exploration of space, enabling missions of scientific discovery to destinations across the solar system by providing electrical power to explore remote, challenging and extreme environments. In particular, RPS enable deep space missions where increased heliocentric distances reduce the ability of solar power to adequately meet spacecraft and instruments power requirements. Some previous notable missions that were enabled by RPS include Nimbus III, the Apollo Sur-face Experiments, the Pioneers 10 and 11, the Viking Mars Landers, Galileo, Ulysses, Cassini, New Horizons and Curiosity. The current operating set of missions that are enabled by RPS are Voyagers 1 and 2, Cassini, New Hori-zons, and Curiosity. The Multi-Mission Radioisotope Thermal Generator (MMRTG) is the current RPS used for mis-sions. An enhanced version of this generator outfitted with higher efficiency thermoelectrics is under development for potential use in the future. There are also current development efforts underway by NASA to explore dynamic power conversion systems such as Brayton and Stirling. This paper will compare and contrast the pros and cons of a generalized dynamic system with the current state-of-the-art thermoelectric static conversion system. The reason for this paper is to calmly analyze the pros and cons so that all the pertinent factors are placed under the same examination light with equal brightness. As with any purchase made the “newness” of certain items sometimes overrides a needed desire to fully vet all characteristics of the merchandise so that the best choice is made. The following areas will be specifically examined and discussed as they may impact the choice of radioisotope power system for a specific mission: • Projected power output to weight ratio (We/kg) • Amount of Pu-238 required per a fixed power output • Excess heat available to be rejected or used • Fueling operations during assembly by the Department of Energy (DOE) • Planetary protection protocol during fueling and testing of RPS • Transportation operations from DOE assembly site to Kennedy Space Center (KSC) • Remote operations at KSC prior to launch during Assembly Test and Launch Operations (ATLO) phase o Timing of the placement of the RPS onto spacecraft/rover o Off-normal operations at KSC

99 GENERAL AND MISCELLANEOUS↗

Methodology for back-contamination risk assessment for a Mars sample return mission

The risk of back-contamination from Mars Surface Sample Return (MSSR) missions is assessed. The methodology is designed to provide an assessment of the probability that a given mission design and strategy will result in accidental release of Martian organisms acquired as a result of MSSR. This is accomplished through the construction of risk models describing the mission risk elements and their impact on back-contamination probability. A conceptual framework is presented for using the risk model to evaluate mission design decisions that require a trade-off between science and planetary protection considerations.

Merkhofer, M. W.↗