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At least 343 records · Page 19

Ebola and Marburg virus matrix layers are locally ordered assemblies of VP40 dimers

Filoviruses such as Ebola and Marburg virus bud from the host membrane as enveloped virions. This process is achieved by the matrix protein VP40. When expressed alone, VP40 induces budding of filamentous virus-like particles, suggesting that localization to the plasma membrane, oligomerization into a matrix layer, and generation of membrane curvature are intrinsic properties of VP40. There has been no direct information on the structure of VP40 matrix layers within viruses or virus-like particles. We present structures of Ebola and Marburg VP40 matrix layers in intact virus-like particles, and within intact Marburg viruses. VP40 dimers assemble extended chains via C-terminal domain interactions. These chains stack to form 2D matrix lattices below the membrane surface. These lattices form a patchwork assembly across the membrane and suggesting that assembly may begin at multiple points. Our observations define the structure and arrangement of the matrix protein layer that mediates formation of filovirus particles.

59 BASIC BIOLOGICAL SCIENCES↗

WHOLE CELL TOMOGRAPHY/MOLECULAR BIOLOGY/STRUCTURAL BIOLOGY: Affordable x-ray microscopy with nanoscale resolution

Biological research spans 10 orders of magnitude from angstroms to meters. While electron microscopy can reveal structural details at most of these spatial length scales, transmission electron tomography only reliably reconstructs three-dimensional (3-D) volumes of cellular material with a spatial resolution between 1-5 nm from samples less than 500 nm thick1. Most biological cells are 2-30 times thicker than this threshold, which means that a cell must be cut into consecutive slices with each slice reconstructed individually in order to approximate the contextual information of the entire cell. Fortunately, due to a larger penetration depth2, X-ray computed tomography bypasses the need to physically section a cell and enables imaging of intact cells and tissues on the micrometer or larger scale with tens to hundreds of nanometer spatial resolution. While the technique of soft x-ray microscopy has been extensively developed in synchrotron facilities, advancements in laboratory x-ray source designs now increase its accessibility by supporting commercial systems suitable for a standard laboratory. In this paper, we highlight a new commercial compact cryogenic soft x-ray microscope designed for a standard laboratory setting and explore its capabilities for mesoscopic investigations of intact prokaryotic and eukaryotic cells.

femtosecond crystallography↗

Irradiated Microsphere Gamma Analyzer for Examination of Particle Fuel

Fabrication of the first series of fuel compacts for the current US tristructural isotropic (TRISO) coated particle fuel development and qualification effort was completed at Oak Ridge National Laboratory (ORNL) in 2006. In November of 2009, after almost 3 years and 620 effective full power days of irradiation in the Advanced Test Reactor at Idaho National Laboratory (INL), the first Advanced Gas Reactor irradiation test (AGR-1) was concluded. Compacts were irradiated at a calculated timeaveraged, volume-averaged temperature of 955–1136°C to a burnup ranging from 11.2–19.5% fissions per initial metal atom and a total fast fluence of 2.2–4.3·1025 n/m2 [1]. No indication of fission product release from TRISO coating failure was observed during the irradiation test, based on real-time monitoring of gaseous fission products. Post-irradiation examination (PIE) and hightemperature safety testing of the compacts has been in progress at both ORNL and INL since 2010, and have revealed small releases of a limited subset of fission products (such as silver, cesium, and europium). Past experience has shown that some elements can be released from TRISO particles when a defect forms in the SiC layer, even when one or more pyrocarbon layers remain intact and retain the gaseous fission products. Some volatile elements can also be released by diffusion through an intact SiC layer during safety testing if temperatures are high enough and the duration is long enough. In order to understand and quantify the release of certain radioactive fission products, it is sometimes necessary to individually examine each of the more than 4000 coated particles in a given compact. The Advanced Irradiated Microsphere Gamma Analyzer (Advanced- IMGA) was designed to perform this task in a remote hot cell environment. This paper describes the Advanced- IMGA equipment and examination process and gives results for a typical full compact evaluation.

11 NUCLEAR FUEL CYCLE AND FUEL MATERIALS↗

Experimental Study for Effects of Ballooning and Power Peak on a Coolability of Fuel Rod Bundle

For a large break loss-of-coolant accident (LB LOCA) in a pressurized water reactor, the clad temperature increases until the reflood phase. This causes ballooned fuel rods and fuel relocation, thereby reducing flow passage area of sub-channel and redistributing the flow and heat transfer in sub-channels. The fuel relocation increase the power at the ballooned region and temperature on the fuel rod. The research on the coolability of the deformed fuel and fuel relocation is very important for a prevention and mitigation of the severe accidents. In the present study, the reflood experiments were performed to investigate the coolability in the ballooned geometry and fuel relocation condition during the LB LOCA for Korean nuclear power plant, APR1400. The coolability experiments were carried out using three kinds of test sections. The first experiments using 6x6 intact rod bundle were performed as a base case. The second experiments simulated the coolability in the deformed fuel using 5x5 ballooned rod bundle. The last experiments were performed using another ballooned 5x5 rod bundle with local power increase to simulate fuel relocation. The fuel relocation phenomena increase the peak cladding temperatures compared with intact and ballooned only rod bundles.

22 GENERAL STUDIES OF NUCLEAR REACTORS↗

AGR-2 TRISO Fuel Post-Irradiation Examination Final Report

An array of post-irradiation exams and experiments were employed to assess the irradiation performance and high-temperature behavior of AGR-2 UCO TRISO fuel produced in an engineering-scale coater. Work was also conducted on UO2 TRISO fuel produced for comparison with the AGR UCO fuel. Emphases we replaced on enumerating the frequency of SiC layer failures (failure of the SiC layer with at least one PyC layer remaining intact) and TRISO failures (failure of all three dense layers) and evaluating the retention of key fission products within the fuel. Examinations of the fuel kernel and coating morphologies were used to understand the features typical of normal intact fuel, degraded or failed fuel, and mechanisms of degradation. Radiochemical analyses were used to determine their retention within the fuel. The results confirm excellent performance of the fuel that exceeds historical HTGR design requirements with significant margin.

11 NUCLEAR FUEL CYCLE AND FUEL MATERIALS↗

Direct determination of antibody chain pairing

Methods are described for using a combination of mass spectroscopic and proteomic approaches for identifying the specific pairing of heavy and light chains for an intact antibody, an antibody fragment, a mixture of intact antibodies, or a mixture of antibody fragments.

Shaw, Jared B.↗

In-Pile testing of CrN, TiAIN, and AlCrN coatings on Zircaloy cladding in the Halden reactor - 2016-0011

CrN coating came out as superior and remained completely intact after the irradiation, as evidenced by SEM analysis. Based on these promising results, an in-pile experiment with coated Zircaloy claddings (containing fuel) was performed in the Halden reactor in PWR conditions in 2014. The experimental rig contained four Zircaloy-4-clad fuel rods-three coated (CrN, TiAlN, and AlCrN) and one uncoated. Due to a mechanical deformation of tubes for cooling water within the rig, the fuel rods were insufficiently cooled, resulting in a higher-than-normal cladding temperature. Nevertheless, an SEM examination showed that the CrN coating largely remained intact apart from several local spots where the coating had cracked or disappeared. Underneath the cracked coating, oxide formation was observed in the Zircaloy-4 cladding. The TiAlN and AlCrN coatings both disappeared after the irradiation. (authors)

36 MATERIALS SCIENCE↗

Dry Storage of Encapsulated Defective Fuel Rods at Doel 1 and 2 in Belgium - 17581

The project of removing all fissile and non-fissile material out of the spent fuel ponds of Belgian Doel power plant is on-going and in that framework, a solution was needed for evacuating all used fuel elements from Doel 1 and 2 storage pool, including Defective Fuel Assemblies (DFA) and Defective Fuel Rods (DFR). Following the moving out of storage pool, all used fuel elements are intended to be loaded in dry storage casks, this used fuel management option being the one implemented today in Belgium while waiting for final management. Synatom, in charge of all fuel cycle related activities for the nuclear power plants of Belgium and Engie-Electrabel, the operator of both Belgium power plants (Doel and Tihange), had then to find an industrial solution for emptying Doel 1 and 2 used fuel pool and loading DFR in dry storage casks. After having thoroughly reviewed the possible options for implementing dry storage of DFR, Synatom selected AREVA technology. It consists in: - encapsulating each DFR by loading the fuel rod in a capsule, drying the fuel rod using a process which allows to physically verify with high accuracy the residual water content inside the DFR, and welding the capsule, - assembling the capsules in a capsule canister (a skeleton with external dimensions similar to fuel assembly's) and - transferring this capsule canister along with intact fuel assemblies in an associated dry transport and storage (dual purpose) cask, as already used for interim storage of used fuel at Doel site. The French as well as the Belgian competent authorities are parties to the licensing process project. The purpose of the paper is to present the on-going industrial project related to dry storage of dried and encapsulated DFR - along with intact used fuel in dual purpose casks - at Doel 1 and 2 in Belgium, its challenges and progress. The paper presents: - why the drying method, welded capsule and associated cask is the best available technology for dry storage of DFR; - a focus on fuel assembly repair operations (initial step before encapsulation) per-formed in 2015 which consisted in successful extraction of four DFR from their original fuel assembly, using proven and experienced methods and tools; - in conclusion, an update of Doel 1 and 2 DFR dry storage project: reaching of major qualifications and licensing milestones and overview of the project perspectives. (authors)

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗

Drying Simulated Advanced Gas Reactor Fuel: Proving the Concept - 17637

Spent Nuclear Fuel (SNF) reprocessing is due to cease in the UK in 2018 and a new interim storage method may be required if it is found that pond storage is not viable beyond the 25 year period which is currently understood to be safe. Dry storage has been used elsewhere in the world for zircaloy and aluminium clad fuel and has been proposed for use in the UK, however since the UK's fuel is predominantly stainless steel (SS) clad, testing will need to be carried out to ensure that drying methods are suitable. This will require representative samples for macro scale drying tests. To date, little long term stored fuel has been examined. As a result, work has been carried out to try and ascertain whether the corrosion associated with long term storage leads to significant bound water and whether through wall cracks can be formed in intact lengths of SS tube. It was found that unlike an aluminium simulant, corroded SS showed no sign of water loss as a result of oxide dehydration during controlled drying by thermogravimetric analysis, and consequently bound water could be disregarded. It was also found that through wall cracks could be produced in an intact length of SS tube which would be suitable for macro scale drying tests. Some initial macro scale drying tests have been carried out using an alternative test piece with preformed cracks, and three distinct drying regimes to complete dryness have been detected. (authors)

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗

Solutions for management of defective fuel assemblies - 15318

Defective fuel management is a major challenge for nuclear operators when the time comes for finding a long-term solution for their used (or spent) fuel assemblies. This question of defective fuel management can be raised in several cases: - For optimizing the remaining operating costs of shutdown reactors, the spent fuel pools must be emptied as soon as possible; a solution needs therefore to be found for evacuating all the spent fuel assemblies, defective fuel assemblies and in particular defective fuel rods included; - Some utilities are today facing a saturation of their reactor spent fuel pool; evacuating the defective fuel assemblies and extracted defective fuel rods allows optimizing the space in the pool. Whereas the solutions for managing intact used fuel assemblies are well-known, a specific technical solution must be implemented for managing the defective spent fuel assemblies as well. The purpose of this paper is to describe the existing solutions for the management of all types of defective fuel assemblies. Interim Dry Storage Solution Interim dry storage of defective spent fuel assemblies is being implemented for decades in the USA. Specific operations are performed for preparing the defective fuel assemblies for interim dry storage and they are then stored with intact used fuel assemblies in the dry storage cask. The advantage of such a solution is its short-term cost effectiveness. The paper presents the existing dry interim storage solutions for defective used fuel rods, the way they are implemented, the associated operational experience. Long-term Dry Storage Solution A long-term dry storage solution for defective fuel assemblies is being developed, compliant with direct disposal requirements. The solution is based on the encapsulation of each defective fuel rod into a fuel rod capsule and an associated specific dry storage licensing approach: this is the best available technology regarding long term storage safety requirements. The paper presents this unique capsule technology, the transport and storage casks that can be used for transportation of defective fuel rods to the storage facility, and the associated transport licensing approach currently used in Europe. Treatment Solution. The most definitive way to get rid of defective fuel assemblies is their treatment (or reprocessing) in a treatment plant. There is then no need to assess the behaviour of these specific used fuel assemblies as for an interim storage facility or a final repository. The advantage of such a solution is its sustainable approach. The paper presents the existing transport and treatment solutions, the way they are implemented, the associated operational experience. (authors)

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗

Analysis of Legacy {sup 85}Kr Waste Form Samples - 16319

In the late 1970's, the Idaho Chemical Processing Plant conducted an R and D program on krypton encapsulation. Legacy samples composed of {sup 85}Kr incorporated in solid zeolite 5A material, and five small metal tubes containing a mixture of the zeolite combined with a glass matrix resulting from hot isostatic pressing, have been preserved. These samples were shipped to Oak Ridge National Laboratory (ORNL) in mid-FY 2014. After receipt and repackaging, the samples were examined by x-ray imaging and x-ray tomography. Two of the capsules appear to contain an amorphous mass within the capsules. An image of one capsule clearly shows the saw marks on the capsule and a quantity of loose pellet or bead-like material remaining in the capsule. An image of another capsule shows similar bead-like material within the intact capsule. One of the capsules was previously opened. The end of this capsule appears to have been cut off, and there are additional saw marks on the side of the capsule. X-ray tomography allowed the capsules to be viewed along the three axes. Of most interest was determining whether there was any residual material in the closed end of this capsule. The images confirmed the presence of residual material within this capsule. The material appears to be compacted but still retains some of the bead-like morphology. Based on the initial nondestructive assay (NDA) and the fact that there are two obviously breached samples, a plan was developed for exploratory tests that would be conducted with the breached specimens before opening the three intact capsules. Preparations are being made to determine the fraction of krypton/xenon remaining in the waste form matrix and the amount of rubidium remaining in the waste form matrix. The inner surface of the breached capsules will be examined for corrosion. (authors)

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗

Nuclear thermal design of high temperature gas-cooled reactor with SiC/C mixed matrix fuel compacts - 18410

Japan Atomic Energy Agency (JAEA) has started R and D on SiC/C mixed matrix fuel element of high temperature gas-cooled reactors (HTGRs) to improve oxidation resistance of fuel. Nuclear thermal design of HTGR with SiC/C mixed matrix fuel compacts was carried out as a part of above R and Ds. Nuclear thermal design was carried out based on a small sized HTGR for developing countries, HTR50S, which thermal power is 50 MW and burn-up period is 2 years. Maximum enrichment of uranium is set to be 10 wt%, because coated fuel particles with 10 wt% uranium have been fabricated in Japan. Numbers of kinds of enrichment and burnable poisons (BPs) were set to be same as those of original HTR50S (3 and 2, respectively). We succeeded in nuclear thermal design of a small sized HTGR which performance was equivalent to original HTR50S, with SiC/C mixed matrix fuel compacts. Based on nuclear thermal design, intactness of coated fuel particles was evaluated to against internal pressure increase by burnup. It was shown that intactness was kept for burnup condition of HTR50S. (authors)

11 NUCLEAR FUEL CYCLE AND FUEL MATERIALS↗

Initial results from safety testing of US AGR-2 irradiation test fuel - 18574

Two cylindrical compacts containing tri-structural isotropic (TRISO)-coated particles with kernels that contained a mixture of uranium carbide and uranium oxide (UCO) and two compacts with UO{sub 2}-kernel TRISO particles have undergone 1600 deg. C safety testing. These compacts were irradiated in the US Advanced Gas Reactor Fuel Development and Qualification Program's second irradiation test (AGR-2). The time-dependent releases of several radioisotopes ({sup 110m}Ag, {sup 134}Cs, {sup 137}Cs, {sup 154}Eu, {sup 155}Eu, {sup 90}Sr, and {sup 85}Kr) were monitored while heating the fuel specimens to 1600 deg. C in flowing helium for 300 h. The UCO compacts behaved similarly to previously reported 1600 deg. C-safety-tested UCO compacts from the AGR-1 irradiation. No failed TRISO or failed SiC were detected (based on krypton and cesium release), and cesium release through intact SiC was very low. Release behavior of silver, europium, and strontium appeared to be dominated by inventory originally released through intact coating layers during irradiation but retained in the compact matrix until it was released during safety testing. Both UO{sub 2} compacts exhibited cesium release from multiple particles whose SiC failed during the safety test. Europium and strontium release from these two UO{sub 2} compacts appeared to be dominated by release from the particles with failed SiC. Silver release was characteristically similar to the release from the UCO compacts in that an initial release of the majority of silver trapped in the matrix occurred during ramping to 1600 deg. C. However, additional silver release was observed later in the safety testing due to the UO{sub 2} TRISO with failed SiC. Failure of the SiC layer in the UO{sub 2} fuel may have been dominated by CO corrosion, as opposed to the palladium degradation observed in AGR-1 UCO fuel. (authors)

21 SPECIFIC NUCLEAR REACTORS AND ASSOCIATED PLANTS↗

A study on the criticality safety for the direct disposal of used nuclear fuel in Japan: Application of burnup credit to the criticality safety evaluation for the disposal canister - 14049

Japan has recently started the technical development of the direct disposal of used nuclear fuel (UNF) to prepare various disposal options. Unlike the disposal of radioactive waste from UNF reprocessing, criticality safety is important here because of the presence of a certain amount of fissile nuclides in UNF. This paper provides an outline of the research to be addressed in this field and the relevant studies in Japan. It especially includes the first result of a criticality safety evaluation of a disposal canister model adopting burnup credit. In this evaluation, it was assumed that a steel disposal canister loaded with fuel assemblies was surrounded by a clay buffer material, and the fuel assemblies and the disposal canister were intact. Conditions representative of discharged pressurized-water reactor (PWR) fuel were applied in this study - initial U-235 enrichment of 4.5 wt% and burnup of 45 GWd/t. The isotopic composition of the burnt fuel was evaluated using SWAT3.1 code. In the depletion calculation, conservative parameters were applied within the typical PWR operation range. The effective neutron multiplication factor (k{sub eff}) was then calculated using a continuous-energy Monte Carlo code MVP-2.0, adopting the JENDL-4.0 nuclear data library. Uncertainty in k{sub eff} due to depletion calculation errors was evaluated using the correction factors of isotopic composition, which were obtained via multiple comparisons between the calculated and measured isotopic concentrations of irradiated fuels. In addition, the effects of axial burnup profile and horizontal burnup gradient on k{sub eff} were evaluated using the open burnup distribution database. The k{sub eff} including the uncertainties and conservatism was found to be below 0.95 for both actinide-only and actinide and fission product considerations when the fuel assemblies and the disposal canister were assumed to be intact. (authors)

11 NUCLEAR FUEL CYCLE AND FUEL MATERIALS↗

Criticality Analysis of FSV Spent Nuclear Fuel in the DOE Standard Canister

The U.S. Department of Energy (DOE) is responsible for managing over 300 types of spent nuclear fuel (SNF). To manage this large variety of fuel types, DOE plans to employ standardized canisters for the transportation, long-term storage, and eventual disposal of SNF. Idaho National Laboratory is currently supporting DOE’s SNF Packaging Demonstration Project, in which Fort Saint Vrain (FSV) fuel assemblies will be loaded into a DOE Standard Canister. This paper presents criticality calculations demonstrating that all four or five FSV fuel assemblies loaded into the DOE Standard Canister will remain subcritical in any expected normal or credible abnormal conditions. Previous criticality analyses were performed for one FSV fuel assembly and 12 Peach Bottom Core 2 fuel elements loaded into a DOE Standard Canister. This paper covers the criticality analysis performed for loading both four and five FSV fuel assemblies into a DOE Standard Canister. Various intact and degraded mode configurations were modeled in conducting the criticality calculations. This analysis encompassed three different configurations: (1) a single DOE Standard Canister loaded into a concrete storage overpack, (2) seven DOE Standard Canisters loaded into a concrete storage overpack, and (3) nine DOE Standard Canisters loaded into a concrete storage overpack. The overpack dimensions were varied for each of the three configurations, and transport, storage, and disposal scenarios were analyzed for each configuration. For transport scenarios, a pair of degradation cases was analyzed. In the first case, the fuel compacts became degraded and were removed from the fuel block, then deposited at the bottom of a horizontally placed canister, thereby simulating a drop event. The canister was considered to remain intact. In the second case, the spacing between horizontally placed canisters in a nine-canister overpack was reduced such that the canisters were piled on top of each other, simulating a drop event. For this case, no degradation of the canister internals or fuel was considered. For storage scenarios, the water moderator location in the system was varied to enable identification of the most reactive configurations. Dry and wet conditions were analyzed for the fuel materials, canister, and overpack. For disposal scenarios, two degradation cases were analyzed. In the first, the stainless-steel internals of the canister degraded to either hematite or goethite under both dry and wet conditions. In the second case, degraded FSV fuel formed a uranium-water slurry that filled the coolant/void holes. None of the cases presented exceeded the application specific upper subcritical limit.

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗

Structural, Criticality, and Radiation Dose Calculations to Support SNF Loading into a DOE Standard Canister

The DOE Standard Canister Demonstration Project includes the development of an internal support structure (ISS) for the 4.6-m-long and 45.7-cm-diameter canister. This study presents structural evaluations, criticality safety assessments, and dose rate calculations conducted within the scope of the ISS design process to support smooth canister loading operations and to ensure safe storage, transportation, and disposal of Peach Bottom 1 Core II (PB2) and Fort St. Vrain (FSV) SNF, currently stored at INL’s CPP-603 facility. The ISS includes a 316L stainless-steel basket that holds 12 PB2 SNF rods. The PB2 basket rests on top of six individual, 78.7-cm-long, 316L stainless-steel columns that are equally spaced and welded to the inner canister wall at the lower end of the shell. These columns represent the FSV basket and can hold one FSV SNF element. An A92014 T6 aluminum spacer disc is bolted to the bottom plate of the PB2 basket to vertically restrain the FSV SNF element after the PB2 basket is placed inside the canister above the FSV basket. The structural evaluations of the ISS followed applicable ASME BPVC.III.3 guidelines and included finite element (FE) analyses of the PB2 basket structure; analyses of welds and bolds; buckling analyses of selected components, and acceptability assessments of the expected basket deformations under loading operations. The criticality safety assessments used the Monte Carlo N-Particle (MCNP) software architecture Version 6.2, including ENDF/B-V continuous-energy cross-section libraries, considering intact SNF in a single storage overpack or two multistorage overpack configurations and intact or failed SNF configured for disposal. The dose rate computations are based on source terms taken from the DOE Spent Fuel Database. The isotopic composition was decay corrected for the year 2022 using the ORIGEN module in the SCALE suite. A 19-group photon spectrum and a 27-group neutron-source spectrum were generated and used in MCNP to calculate estimated dose-equivalent rates, both on DOE Standard Canister contact and at a radial distance of 1 m from the canister surface. The results of this study indicate a structurally sound system that can uphold its criticality safety functions throughout its intended operational phases. Furthermore, this study provides confidence that sufficient radiological protection is technically achievable.

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗

Structural, Criticality, and Radiation Dose Calculations to support SNF Loading into a DOE Standard Canister

The DOE Standard Canister Demonstration Project includes the development of an internal support structure (ISS) for the 4.6-m long and 45.7-cm diameter canister. This study presents structural evaluations, criticality safety assessments, and dose rate calculations conducted within the scope of the ISS design process to support smooth canister loading operations and to ensure safe storage, transportation, and disposal of Peach Bottom 1 Core II (PB2) and Fort St. Vrain (FSV) SNF, currently stored at the CPP-603 facility of the INL. The ISS includes a 316L stainless steel basket that holds twelve PB2 SNF rods. Further, six individual, 78.7-cm long, 316L stainless steel columns are equally spaced and welded to the inner canister wall at the lower end of the shell. These columns represent the FSV basket and can hold one FSV SNF element. An A92014 T6 aluminum spacer disc is bolted to the bottom plate of the PB2 basket to vertically restrain the FSV SNF element after the PB2 basket is placed inside the canister on top of the FSV basket. The structural evaluations of the ISS followed applicable ASME BPVC.III.3 guidelines and included finite element (FE) analyses of the PB2 basket structure; analyses of welds and bolds; buckling analyses of selected components, and acceptability assessments of the expected basket deformations under loading operations. The criticality safety assessments used the Monte Carlo N-Particle (MCNP) software architecture version 6.2 including ENDF/B-V continuous energy cross-section libraries, considering intact SNF in a single storage overpack or two multi-storage overpack configurations, and intact or failed SNF configured for disposal. The dose rate computations are based on source terms taken from the DOE Spent Fuel Database. The isotopic composition was decay corrected for the year 2022 using the ORIGEN module in the SCALE suite. A 19-group photon spectrum and 27-group neutron-source spectra were generated and used in MCNP to calculate estimated dose-equivalent rates, both on DOE Standard Canister contact and at a radial distance of from the canister surface. The results of this study indicate a structurally sound system that can uphold its criticality safety functions throughout its intended operational phases. Further, they increase confidence that sufficient radiological protection is technically achievable.

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗

High-resolution ultrahigh-pressure long column reversed-phase liquid chromatography for top-down proteomics

We report development of an approach providing high-resolution RPLC of proteins and its utility for mass spectrometry-based top-down proteomics. A chromatographic peak capacity of ~450 was achieved for proteins and large polypeptides having MWs up to 43 kDa in the context of proteomics applications. RPLC column lengths from 20 to 200 cm, particle sizes from 1.5 to 5 m, bonding alkyl chains from C1 to C2, C4, C8, and C18, and particle surface structures that spanned porous, superficially porous (porous shell, core-shell), and nonporous were investigated at pressures up to14K psi. Column length was found as the most important factor for >20 kDa proteins in gradient RPLC, and shortening column length degraded RPLC resolution and sensitivity regardless of the size and surface structure of the packing particles used. The alkyl chains bonded to the silica particle surface significantly affected the RPLC recovery and efficiency, and short alkyl C1-C4 phases provided higher sensitivity and resolution than C8 and C18 phases. Long gradient separations (e.g., >10 hours) with long columns (e.g., 100 cm) were particularly effective in conjunction with use of high accuracy mass spectrometers (e.g., the Orbitrap Elite) for top-down proteomics with improved proteoform coverage by allowing multiple HCD, CID, and ETD dissociation modes. It was also found that HCD produced small fragments useful for proteoform identification, while low energy CID and ETD often complemented HCD by providing large fragments.

columns and stationary phases↗