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At least 343 records · Page 19

Ecosystem Fabrication (EcoFAB) Protocols for The Construction of Laboratory Ecosystems Designed to Study Plant-microbe Interactions

Beneficial plant-microbe interactions offer a sustainable biological solution with the potential to boost low-input food and bioenergy production. A better mechanistic understanding of these complex plant-microbe interactions will be crucial to improving plant production as well as performing basic ecological studies investigating plant-soil-microbe interactions. Here, a detailed description for ecosystem fabrication is presented, using widely available 3D printing technologies, to create controlled laboratory habitats (EcoFABs) for mechanistic studies of plant-microbe interactions within specific environmental conditions. Two sizes of EcoFABs are described that are suited for the investigation of microbial interactions with various plant species, including Arabidopsis thaliana, Brachypodium distachyon, and Panicum virgatum. These flow-through devices allow for controlled manipulation and sampling of root microbiomes, root chemistry as well as imaging of root morphology and microbial localization. This protocol includes the details for maintaining sterile conditions inside EcoFABs and mounting independent LED light systems onto EcoFABs. Detailed methods for addition of different forms of media, including soils, sand, and liquid growth media coupled to the characterization of these systems using imaging and metabolomics are described. Together, these systems enable dynamic and detailed investigation of plant and plant-microbial consortia including the manipulation of microbiome composition (including mutants), the monitoring of plant growth, root morphology, exudate composition, and microbial localization under controlled environmental conditions. We anticipate that these detailed protocols will serve as an important starting point for other researchers, ideally helping create standardized experimental systems for investigating plant-microbe interactions.

54 ENVIRONMENTAL SCIENCES↗

Material interactions with the Low Earth Orbital (LEO) environment: Accurate reaction rate measurements

To resolve uncertainties in estimated LEO atomic oxygen fluence and provide reaction product composition data for comparison to data obtained in ground-based simulation laboratories, a flight experiment has been proposed for the space shuttle which utilizes an ion-neutral mass spectrometer to obtain in-situ ambient density measurements and identify reaction products from modeled polymers exposed to the atomic oxygen environment. An overview of this experiment is presented and the methodology of calibrating the flight mass spectrometer in a neutral beam facility prior to its use on the space shuttle is established. The experiment, designated EOIM-3 (Evaluation of Oxygen Interactions with Materials, third series), will provide a reliable materials interaction data base for future spacecraft design and will furnish insight into the basic chemical mechanisms leading to atomic oxygen interactions with surfaces.

Visentine, James T.↗

Methanol Steam Reforming on Ru/m-ZrO 2 : Sodium Promotion of the CO 2 -Forming Pathway

Sodium (Na) promotion of Ru/m-ZrO 2 was investigated to elucidate how an alkali modification tunes selectivity in methanol steam reforming (MSR). H 2 -TPR/XANES/EXAFS show that Na increases surface basicity and strengthens Ru–O interactions, shifting RuO x reduction and H 2 spillover to a higher temperature. DRIFTS reveals Na-induced red shifts of the formate ν(CH) band and changes in OCO vibrational splitting, consistent with weakening of the formate C–H bond and an altered binding geometry. CO 2 -TPD confirms a monotonic shift toward stronger basic sites with increasing Na concentrations. Under MSR conditions, Na selectively increases CO2 concentration at the expense of CO. At ~80% conversion and 325 °C, CO 2 selectivity increases from 12.0% (unpromoted) to 16.2, 21.0, and 26.5% for 0.5, 1.0, and 1.8% Na, respectively; at ~300 °C and ~66–69% conversion, CO 2 selectivity increases from 8.6% to 23.7% at 1.8% Na. Transient MSR experiments further show earlier and larger H 2 evolution upon Na addition, corroborating the promotion of the dehydrogenation/decarbonylation route to CO 2 + H 2 . We propose that Na increases basicity and modifies the Ru–support interface to favor formate dehydrogenation/decarboxylation, thereby increasing the H 2 yield and lowering CO formation. Ru’s higher-energy, less occupied d-band stabilizes CO and oxygenated intermediates more strongly in the reforming environment, making the CO-forming pathway more resistant to suppression than on Pt.

CO2 selectivity↗

Quasiparticle-phonon interaction in the theory of finite Fermi systems

Within the Green's function method and on the basis of the method developed by V.A. Khodel for analyzing anharmonic effects, effects of quasiparticle-phonon interaction in the second order in the amplitude of phonon production are studied in two problems as a natural development of A.B. Migdal's theory of finite Fermi systems. Transitions between excited states and static moments of magic and nonmagic nuclei in excited states, each of which is described in the random-phase approximation, are considered. The results for this problem are found to differ considerably from those in the quasiparticle random-phase approximation. The inclusion of all second-order anharmonic effects in the extended theory of finite Fermi systems that extends the standard theory of finite Fermi systems to the case of taking into account quasiparticle-phonon interaction in order to describe excited states, but which does not take into account all such effects, is also considered. They are taken into account at a level that makes it possible to calculate static moments of odd nuclei-more precisely, the respective equation for the vertex function, which, in the theory of finite Fermi systems, is a basic ingredient that describes the interaction of a nucleus with an external field, is derived. Some numerical results obtained within the recently implemented self-consistent version of the extended theory of finite Fermi systems are also presented for 15 stable and unstable tin isotopes. These results give sufficient grounds to conclude that phenomenological systematics are inapplicable to giant dipole resonances in neutron-rich isotopes. The cross sections for radiative neutron capture that are calculated by usingmicroscopic strength functions for the neutron-rich isotopes 132Sn and 150Sn differ strongly from the cross sections calculated on the basis of a phenomenological description of giant dipole resonances. These results are of paramount importance for astrophysics and for the theory of nuclear data for reactors since they highlight the inapplicability of phenomenological systematics to giant dipole resonances and the need for self-consistent calculations for neutron-rich nuclei.

73 NUCLEAR PHYSICS AND RADIATION PHYSICS↗

Interesting Scientific Questions Regarding Interactions in the Gas-aerosol-cloud System

The growth of human population and their use of land, food and energy resources affect the Earth's atmosphere, biosphere and oceans in a complex manner. Many important questions in earth sciences today deal with issues regarding the impact of human activities on our immediate and future environment, ranging in scope from local (i.e. air pollution) to global (i.e. global warming) scale problems. Because the mass of the Earth's atmosphere is negligible compare to that found in the oceans and the biosphere, the atmosphere can respond quickly to natural and/or manmade perturbations. For example, seasonal 'ozone hole' formation in the Antarctic is a result of manmade CFC emissions in just the last 40 years. Also, the observed rise in global temperatures (known as global warming) is linked to a rapid increase in carbon dioxide and other greenhouse gas concentrations (emitted primarily by combustion processes) over the last century. The Earth's atmosphere is composed of a mixture of gases, aerosol and cloud particles. Natural and anthropogenic emissions of gases and aerosols affect the composition of the Earth's atmosphere. Changes in the chemical and physical makeup of the atmosphere can influence how the Earth will interact with the incoming solar radiation and the outgoing infrared radiation and vise versa. While, some perturbations are short-lived, others are long-lived and can affect the Earth's global climate and chemistry in many decades to come, In order to be able to separate the natural effects from anthropogenic ones, it is essential that we understand the basic physics and chemistry of interactions in the gas-aerosol-cloud system in the Earth's atmosphere. The important physics and chemistry that takes place in the coupled gas-aerosol-cloud system as it relates to aircraft observations are discussed.

Tabazadeh, Azadeh↗

Observations of moon-plasma interactions by orbital and surface experiments

Extensive magnetic field observations together with crucial plasma measurements by the Explorer 35 lunar orbiter and Apollo surface and orbital experiments have established the basic nature of the moon's interaction with the solar wind and interplanetary magnetic field. The effective absorption of the incident solar wind by the moon creates a plasma void or cavity behind the moon. The cavity-associated magnetic signature is characterized by an enhancement in magnetic field magnitude B within the cavity as compared with the mean level of B in the surrounding interplanetary plasma and dips or decreases in B near the cavity boundaries with the solar wind. Apollo particle and field measurements on the lunar surface have provided evidence of a regional interaction of the highly conducting solar wind with lunar remanent magnetic fields. Simultaneous plasma and magnetic field data, from the spectrometer and the lunar surface magnetometer at the Apollo 12 location, show the compression of the local remanent field by large solar wind and magnetosheath plasma dynamic pressures.

Schubert, G.↗

Radiative interactions in molecular gases under local and nonlocal thermodynamic equilibrium conditions

Basic formulations, analyses, and numerical procedures are presented to investigate radiative heat interactions in diatomic and polyatomic gases under local and nonlocal thermodynamic equilibrium conditions. Essential governing equations are presented for both gray and nongray gases. Information is provided on absorption models, relaxation times, and transfer equations. Radiative flux equations are developed which are applicable under local and nonlocal thermodynamic equilibrium conditions. The problem is solved for fully developed laminar incompressible flows between two parallel plates under the boundary condition of a uniform surface heat flux. For specific applications, three diatomic and three polyatomic gases are considered. The results are obtained numerically by employing the method of variation of parameters. The results are compared under local and nonlocal thermodynamic equilibrium conditions at different temperature and pressure conditions. Both gray and nongray studies are conducted extensively for all molecular gases considered. The particular gases selected for this investigation are CO, NO, OH, CO2, H2O, and CH4. The temperature and pressure range considered are 300-2000 K and 0.1-10 atmosphere, respectively. In general, results demonstrate that the gray gas approximation overestimates the effect of radiative interaction for all conditions. The conditions of NLTE, however, result in underestimation of radiative interactions. The method developed for this study can be extended to solve complex problems of radiative heat transfer involving nonequilibrium phenomena.

Tiwari, S. N.↗

Heuristic control of the Utah/MIT dextrous robot hand

Basic hand grips and sensor interactions that a dextrous robot hand will need as part of the operation of an EVA Retriever are analyzed. What is to be done with a dextrous robot hand is examined along with how such a complex machine might be controlled. It was assumed throughout that an anthropomorphic robot hand should perform tasks just as a human would; i.e., the most efficient approach to developing control strategies for the hand would be to model actual hand actions and do the same tasks in the same ways. Therefore, basic hand grips that human hands perform, as well as hand grip action were analyzed. It was also important to examine what is termed sensor fusion. This is the integration of various disparate sensor feedback paths. These feedback paths can be spatially and temporally separated, as well as, of different sensor types. Neural networks are seen as a means of integrating these varied sensor inputs and types. Basic heuristics of hand actions and grips were developed. These heuristics offer promise of control dextrous robot hands in a more natural and efficient way.

Bass, Andrew H., Jr.↗

Paired and interacting galaxies: Conference summary

The author gives a summary of the conference proceedings. The conference began with the presentation of the basic data sets on pairs, groups, and interacting galaxies with the latter being further discussed with respect to both global properties and properties of the galactic nuclei. Then followed the theory, modelling and interpretation using analytic techniques, simulations and general modelling for spirals and ellipticals, starbursts and active galactic nuclei. Before the conference the author wrote down the three questions concerning pairs, groups and interacting galaxies that he hoped would be answered at the meeting: (1) How do they form, including the role of initial conditions, the importance of subclustering, the evolution of groups to compact groups, and the fate of compact groups; (2) How do they evolve, including issues such as relevant timescales, the role of halos and the problem of overmerging, the triggering and enhancement of star formation and activity in the galactic nuclei, and the relative importance of dwarf versus giant encounters; and (3) Are they important, including the frequency of pairs and interactions, whether merging and interactions are very important aspects of the life of a normal galaxy at formation, during its evolution, in forming bars, shells, rings, bulges, etc., and in the formation and evolution of active galaxies? Where possible he focuses on these three central issues in the summary.

Norman, Colin A.↗

Review of Aerosol–Cloud Interactions: Mechanisms, Significance, and Challenges

Over the past decade, the number of studies that investigate aerosol-cloud interactions has increased considerably. Although tremendous progress has been made to improve our understanding of basic physical mechanisms of aerosol-cloud interactions and reduce their uncertainties in climate forcing, we are still in poor understanding of (1) some of the mechanisms that interact with each other over multiple spatial and temporal scales, (2) the feedback between microphysical and dynamical processes and between local-scale processes and large-scale circulations, and (3) the significance of cloud-aerosol interactions on weather systems as well as regional and global climate. This review focuses on recent theoretical studies and important mechanisms on aerosol-cloud interactions, and discusses the significances of aerosol impacts on raditative forcing and precipitation extremes associated with different cloud systems. Despite significant understanding has been gained about aerosol impacts on the main cloud types, there are still many unknowns especially associated with various deep convective systems. Therefore, large efforts are needed to escalate our understanding. Future directions should focus on obtaining concurrent measurements of aerosol properties, cloud microphysical and dynamic properties over a range of temporal and spatial scales collected over typical climate regimes and closure studies, as well as improving understanding and parameterizations of cloud microphysics such as ice nucleation, mixed-phase properties, and hydrometeor size and fall speed

Fan, Jiwen↗

Membrane stiffness and myelin basic protein binding strength as molecular origin of multiple sclerosis

Myelin basic protein (MBP) and its interaction with lipids of the myelin sheath plays an important part in the pathology of multiple sclerosis (MS). Previous studies observed that changes in the myelin lipid composition lead to instabilities and enhanced local curvature of MBP-lipid multilayer structures. We investigated the molecular origin of the instability and found that the diseased lipid membrane has a 25% lower bending rigidity, thus destabilizing smooth >1µm curvature radius structures such as in giant unilamellar vesicles. MBP-mediated assembling of lipid bilayers proceeds in two steps, with a slow second step occurring over many days where native lipid membranes assemble into well-defined multilayer structures, whereas diseased lipid membranes form folded assemblies with high local curvature. For both native and diseased lipid mixtures we find that MBP forms dense liquid phases on top of the lipid membranes mediating attractive membrane interactions. Furthermore, we observe MBP to insert into its bilayer leaflet side in case of the diseased lipid mixture, whereas there is no insertion for the native mixture. Insertion increases the local membrane curvature, and could be caused by a decrease of the sphingomyelin content of the diseased lipid mixture. These findings can help to open a pathway to remyelination strategies.

59 BASIC BIOLOGICAL SCIENCES↗

Next-Generation Sequencing for Binary Protein–Protein Interactions

The yeast two-hybrid (Y2H) system exploits host cell genetics in order to display binary protein–protein interactions (PPIs) via defined and selectable phenotypes. Numerous improvements have been made to this method, adapting the screening principle for diverse applications, including drug discovery and the scale-up for proteome wide interaction screens in human and other organisms. Here we discuss a systematic workflow and analysis scheme for screening data generated by Y2H and related assays that includes high-throughput selection procedures, readout of comprehensive results via next-generation sequencing (NGS), and the interpretation of interaction data via quantitative statistics. The novel assays and tools will serve the broader scientific community to harness the power of NGS technology to address PPI networks in health and disease. We discuss examples of how this next-generation platform can be applied to address specific questions in diverse fields of biology and medicine.

quantitative interaction profiles↗

A new dipolar potential for numerical simulations of polar fluids on the 4D hypersphere

We present a new method for Monte Carlo or Molecular Dynamics numerical simulations of three-dimensional polar fluids. The simulation cell is defined to be the surface of the northern hemisphere of a four-dimensional (hyper)sphere. The point dipoles are constrained to remain tangent to the sphere and their interactions are derived from the basic laws of electrostatics in this geometry. The dipole-dipole potential has two singularities which correspond to the following boundary conditions: when a dipole leaves the northern hemisphere at some point of the equator, it reappears at the antipodal point bearing the same dipole moment. We derive all the formal expressions needed to obtain the thermodynamic and structural properties of a polar liquid at thermal equilibrium in actual numerical simulation. We notably establish the expression of the static dielectric constant of the fluid as well as the behavior of the pair correlation at large distances. We report and discuss the results of extensive numerical Monte Carlo simulations for two reference states of a fluid of dipolar hard spheres and compare these results with previous methods with a special emphasis on finite size effects.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

Gamma-Ray Interactions for Reachback Analysts

This presentation is a part of the DHS LSS spectroscopy training course and presents an overview of the following concepts: identification and measurement of gamma rays; use of gamma counts and energies in research. Understanding the basic physics of how gamma rays interact with matter can clarify how certain features in a spectrum were produced.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗

Continuation of full-scale three-dimensional numerical experiments on high-intensity particle and laser beam-matter interactions

We present results from the grant entitled, Continuation of full-scale three-dimensional numerical experiments on high-intensity particle and laser beam-matter interactions. The research significantly advanced the understanding of basic high-energy density science (HEDS) on ultra intense laser and particle beam plasma interactions. This advancement in understanding was then used to to aid in the quest to make 1 GeV to 500 GeV plasma based accelerator stages. The work blended basic research with three-dimensions fully nonlinear and fully kinetic simulations including full-scale modeling of ongoing or planned experiments. The primary tool was three-dimensional particle-in-cell simulations. The simulations provided a test bed for theoretical ideas and models as well as a method to guide experiments. The research also included careful benchmarking of codes against experiment. High-fidelity full-scale modeling provided a means to extrapolate parameters into regimes that were not accessible to current or near term experiments, thereby allowing concepts to be tested with confidence before tens to hundreds of millions of dollars were spent building facilities. The research allowed the development of a hierarchy of PIC codes and diagnostics that is one of the most advanced in the world.

43 PARTICLE ACCELERATORS↗

Opportunities for Undergraduate Research in Nuclear Physics

University of Dallas (UD) physics majors are offered a variety of undergraduate research opportunities in nuclear physics through an established program at the University of Kentucky Accelerator Laboratory (UKAL). The 7-MV Model CN Van de Graaff accelerator and the neutron production and detection facilities located there are used by UD students to investigate how neutrons scatter from materials that are important in nuclear energy production and for our basic understanding of how neutrons interact with matter. Recent student projects include modeling of the laboratory using the neutron transport code MCNP to investigate the effectiveness of laboratory shielding, testing the long-term gain stability of C 6 D 6 liquid scintillation detectors, and deducing neutron elastic and inelastic scattering cross sections for 12 C. Finally, results of these student projects are presented that indicate the pit below the scattering area reduces background by as much as 30%; the detectors show no significant gain instabilities; and new insights into existing 12 C neutron inelastic scattering cross-section discrepancies near a neutron energy of 6.0 MeV are obtained.

73 NUCLEAR PHYSICS AND RADIATION PHYSICS↗