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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 343 records · Page 19
All sourcesNASA NTRSDOE OSTI
NASA NTRS2018

High-precision Method for Cyclic Loading of Small-Animal Vertebrae to Assess Bone Quality

One potentially important bone quality characteristic is the response of bone to cyclic (repetitive) mechanical loading. In small animals, such as in rats and mice, cyclic loading experiments are particularly challenging to perform in a precise manner due to the small size of the bones and difficult-to-eliminate machine compliance. Addressing this issue, we developed a precise method for ex vivo cyclic compressive loading of isolated mouse vertebral bodies. The method has three key characteristics: 3D-printed support jigs for machining plano-parallel surfaces of the tiny vertebrae; pivotable loading platens to ensure uniform contact and loading of specimen surfaces; and specimen-specific micro-CT-based finite element analysis to measure stiffness to prescribe force levels that produce the same specified level of strain for all test specimens. To demonstrate utility, we measured fatigue life for three groups (n = 5-6 per group) of L5 vertebrae of C57BL/6J male mice, comparing our new method against two methods commonly used in the literature. We found reduced scatter of the mechanical behavior for this new method compared to the literature methods. In particular, for a controlled level of strain, the standard deviation of the measured fatigue life was up to 5-fold lower for the new method (F-ratio = 4.9; p < 0.01). The improved precision for this new method for biomechanical testing of small-animal vertebrae may help elucidate aspects of bone quality.

Bone quality↗
NASA NTRS2015

Translational Cell & Animal Research in Space 1965-2011

Translational Cell and Animal Research (TCAR). For nearly 50 years, the NASA Space Biology Program has funded, and Ames Research Center (ARC) has managed, a robust program of fundamental research including studies using a wide range of animal cells, tissues and organisms. Much of this research was conducted on spacecraft in microgravity environments including diverse platforms such as: Gemini Spacecraft, US Biosatellites, Apollo Command Modules, Skylabs, Russian Biosatellites, NASA Space Shuttles, NASA/Mir, and most recently, the International Space Station (ISS). During the Space Shuttle Era (1981–2011), the science of space biology took an enormous step forward with 45 missions that afforded researchers with new opportunities to conduct systematic and complex experiments aimed at a deeper understanding of how life adapts to the space environment. Beginning in the 1990s, the products of these experiments, comprised of research summaries and rare, unused biospecimens, were collected and catalogued within the ARC Life Sciences Data Archiving Office, a branch of NASA’s Life Sciences Data Archive (LSDA) managed from the NASA Johnson Spaceflight Center.

Ronca, April E.↗
NASA NTRSDate not supplied

NASA GeneLab Platform Utilized for Biological Response to Space Radiation in Animal Models

Ionizing radiation from Galactic Cosmic Rays (GCR) is one of the major risk factors that will impact the health of astronauts on extended missions outside the protective effects of Earth’s magnetic field. The NASA GeneLab project has detailed information on radiation exposure using animal models with curated dosimetry information for spaceflight experiments. We analyzed multiple GeneLab omics datasets associated with both ground-based and spaceflight radiation studies that included in vivo and in vitro approaches. A range of ions from protons to iron particles with doses from 0.1 Gy to 1.0 Gy for ground studies and samples flown in Low Earth Orbit (LEO) with total doses of 1.0 mGy to 30 mGy were utilized From this analysis we were able to identify distinct biological signatures associating specific ions with specific biological responses due to radiation exposure in space. For example, we discovered changes in mitochondrial function, ribosomal assembly, and immune pathways as a function of dose. We provided a summary of how the GeneLab’s rich database of omics experiments with animal models can be used to generate novel hypotheses to better understand human health risks from GCR exposures.

Afshin Beheshti↗
NASA NTRS2020

MAGIK Animation of Robotic Operations for Flight SpaceX-22 to ISS

The MAGIK Robotic analysis team provides kinematic feasibility assessments for the ISS and has produced an animation to evaluate Extravehicular Robotics (EVR) activities for the SpaceX (SpX)-22 flight. The animation also displays the current ISS configuration at the time of the flight. Flight SpX-22 includes a SpaceX Cargo Dragon vehicle docked to Node 2 Zenith (N2Z) International Docking Adapter (IDA) and consists of the delivery of the ISS Power Augmentation (IPA) Carrier. Robotic operations shown include the Space Station Remote Manipulator System (SSRMS) Trunk extraction of the IPA Carrier and maneuvering to the install position on the Payload ORU (Orbital Replacement Unit) Accommodation (POA). There is no disposal payload for this flight.

Alexander Radford Fryer↗
NASA NTRS2022

SpaceX-26 Animation - Operations from MAGIK AI-4177: SpX-26 (Launch-6)

The MAGIK Robotic analysis team provides kinematic feasibility assessments for the ISS and has produced an animation to evaluate Extravehicular Robotics (EVR) activities for the SpaceX (SpX)-26 flight. The animation also displays the current ISS configuration at the time of the flight. Flight SpX-26 includes a SpaceX Cargo Dragon vehicle docked to Node 2 Zenith (N2Z) International Docking Adapter (IDA) and consists of the delivery of the ISS Roll Out Solar Array (IROSA) mounted to an ISS Power Augmentation (IPA) Carrier. Robotic operations shown include the Space Station Remote Manipulator System (SSRMS) Trunk extraction of the IPA Carrier and maneuvering to the install position on the Payload ORU (Orbital Replacement Unit) Accommodation (POA). There is no disposal payload for this flight.

ISS↗
NASA NTRS2023

Magik Animation of Robotic Operations for Flight SpaceX-29 to ISS

The MAGIK Robotic analysis team provides kinematic feasibility assessments for the ISS and has produced an animation to evaluate Extravehicular Robotics (EVR) activities for the SpaceX (SpX)-29 flight. The animation also displays the current ISS configuration at the time of the flight. Flight SpX-29 includes a SpaceX Cargo Dragon vehicle docked to Node 2 Forward (N2F) International Docking Adapter (IDA) and consists of the delivery of AWE (Atmospheric Waves Experiment) and Integrated LCRD LEO User Modem Amplifier optical communications Terminal (ILLUMA-T). Robotic operations shown include the Special Purpose Dexterous Manipulator (SPDM) Trunk extraction of ILLUMA-T and maneuvering to the JEM RMS Handoff position, the SPDM Trunk extraction of AWE, maneuvering to Express Logistics Carrier (ELC) 1 Site 3 to remove Space Test Program - Houston 5 (STP-H5), and the installation of AWE to ELC-1 Site 3. There is no disposal payload for this flight.

EVR↗
NASA NTRS2024

MAGIK Animation of Robotic Operations for Flight SpaceX-30 to ISS

The MAGIK Robotic analysis team provides kinematic feasibility assessments for the ISS and has produced an animation to evaluate Extravehicular Robotics (EVR) activities for the SpaceX (SpX)-30 flight. Shown EVR activities are preliminary and final operations will be developed by Flight Operations Directorate (FOD) Robotics (ROBO). The animation also displays the current ISS configuration at the time of the flight. Flight SpX-30 includes a SpaceX Cargo Dragon vehicle docked to Node 2 Zenith (N2Z) International Docking Adapter (IDA) and consists of the delivery of the Pump Module (PM) Integrated Assembly (IA). Robotic operations shown include the Special Purpose Dexterous Manipulator (SPDM) based on the Space Station Remote Manipulator System (SSRMS) for Trunk extraction of the PM IA, maneuvering to the External Stowage Platform 3 (ESP-3), temporary removal of the Control Moment Gyro (CMG) from ESP-3 Site 4, install of the PM IA at ESP-3 Site 3 and re-install of the CMG to ESP-3 Site4. There is no disposal payload for this flight.

ISS↗
DOE OSTI2014

A Simultaneous PET and Fluorescence Optical Tomography Imaging System for Small Animals

For 3D FOT imaging, we have developed an innovative system geometry using a truncated conical mirror, thereby allowing simultaneous viewing of the entire surface of the animal by an EMCCD camera . This geometry, and the small size of the conical mirror (outside diameter 152 mm, total length of 62.5 mm) makes it feasible to insert the conical mirror inside the gantry of our existing microPET II scanner for simultaneous PET and 3D FOT imaging. The first-generation conical mirror was made of aluminum and coated with silver. The outside diameter of the conical mirror is 15.2 cm in diameter, which is 1 mm smaller than the open aperture diameter of microPET II. The axial length of the mirror is 6.25 cm with an axial field of view (FOV) of 6 cm. Shown is a side view schematic and photographs of the imaging system. A 650 laser with a collimation lens at the end of its pigtail fiber was used to excite the fluorescence dye. A bandpass filter following the laser removed light at other wavelengths. Another lens focused the beam into a small 1 mm diameter spot at the mouse surface. Two motorized mirrors scanned the laser beam across the mouse surface. A transparent plastic bed was placed in the center of the conical mirror. Bandpass filters, placed inside a filter wheel, were used to select emission wavelengths. In the front of the filter wheel, a long pass filter was used to remove excitation laser spillover into the measurements at the emission wavelengths. An EMCCD was used to collect the emission photons. Three line pattern lasers on a linear stage were used to extract mouse surface geometry were scanned axially along the mouse to extract the surface geometry. The bottom surface was assumed to be a flat plane based on the geometry of the imaging platform on which the object or animal was placed. To minimize ambient light effects on FOT imaging, the open aperture of the conical mirror and the gap between the FOT enclosure and PET gantry were covered with black cloth.

47 OTHER INSTRUMENTATION↗
DOE OSTI2019

Signal transmitter and methods for transmitting signals from animals

Injectable transmitters are provided that can include a body with the body housing a power source and an oscillator, the injectable transmitter also including an antenna extending from the body, the body and antenna being of sufficient size to be injected through a 9 gauge needle. Radio frequency transmitters are provided that can include a body extending from a nose to a tail with the body housing a power source and RF signal generator components. The power source of the transmitter can define at least a portion of the nose of the body. The transmitters can have an antenna extending from the tail. Methods for attaching a radio frequency (RF) transmitter to an animal are provided. The methods can include providing an RF transmitter and providing an injection device having a needle of gauge of 9 or smaller; providing the RF transmitter into the injection device; and providing the RF transmitter through the 9 gauge or smaller needle and into the animal.

Deng, Z. Daniel↗
DOE OSTI2021

Methods for attaching transmitters to animals

Methods for attaching a radio frequency (RF) transmitter to an animal are provided. The methods can include providing an RF transmitter and providing an injection device having a needle of gauge of 9 or smaller; providing the RF transmitter into the injection device; and providing the RF transmitter through the 9 gauge or smaller needle and into the animal.

Deng, Z. Daniel↗
DOE OSTI2022

Methods for attaching transmitters to animals

Methods for attaching a radio frequency (RF) transmitter to an animal are provided. The methods can include providing an RF transmitter and providing an injection device having a needle of gauge of 9 or smaller; providing the RF transmitter into the injection device; and providing the RF transmitter through the 9 gauge or smaller needle and into the animal.

59 BASIC BIOLOGICAL SCIENCES↗
NASA NTRS2003

Mortality of atomic bomb survivors predicted from laboratory animals

Exposure, pathology and mortality data for mice, dogs and humans were examined to determine whether accurate interspecies predictions of radiation-induced mortality could be achieved. The analyses revealed that (1) days of life lost per unit dose can be estimated for a species even without information on radiation effects in that species, and (2) accurate predictions of age-specific radiation-induced mortality in beagles and the atomic bomb survivors can be obtained from a dose-response model for comparably exposed mice. These findings illustrate the value of comparative mortality analyses and the relevance of animal data to the study of human health effects.

NASA Discipline Radiation Health↗
NASA NTRS2002

Pulmonary venous flow determinants of left atrial pressure under different loading conditions in a chronic animal model with mitral regurgitation

BACKGROUND: The aim of our study was to quantitatively compare the changes and correlations between pulmonary venous flow variables and mean left atrial pressure (mLAP) under different loading conditions in animals with chronic mitral regurgitation (MR) and without MR. METHODS: A total of 85 hemodynamic conditions were studied in 22 sheep, 12 without MR as control (NO-MR group) and 10 with MR (MR group). We obtained pulmonary venous flow systolic velocity (Sv) and diastolic velocity (Dv), Sv and Dv time integrals, their ratios (Sv/Dv and Sv/Dv time integral), mLAP, left ventricular end-diastolic pressure, and MR stroke volume. We also measured left atrial a, x, v, and y pressures and calculated the difference between v and y pressures. RESULTS: Average MR stroke volume was 10.6 +/- 4.3 mL/beat. There were good correlations between Sv (r = -0.64 and r = -0.59, P <.01), Sv/Dv (r = -0.62 and r = -0.74, P <.01), and mLAP in the MR and NO-MR groups, respectively. Correlations were also observed between Dv time integral (r = 0.61 and r = 0.57, P <.01) and left ventricular end-diastolic pressure in the MR and NO-MR groups. In velocity variables, Sv (r = -0.79, P <.001) was the best predictor of mLAP in both groups. The sensitivity and specificity of Sv = 0 in predicting mLAP 15 mm Hg or greater were 86% and 85%, respectively. CONCLUSION: Pulmonary venous flow variables correlated well with mLAP under altered loading conditions in the MR and NO-MR groups. They may be applied clinically as substitutes for invasively acquired indexes of mLAP to assess left atrial and left ventricular functional status.

Pulmonary Veins/physiopathology↗
NASA NTRS2003

Interaction between Japanese flowering cherry trees and some wild animals observed during physiological experiment in fields

We have studied the weeping habit of Japanese flowering cherry tree in the field of Tama Forest Science Garden, Forestry and Forest Products Research Institute at the foot of Mt. Takao. Since cherry trees at various age were the materials for our plant physiology experiments, our studies were conducted in the fields where we experienced certain difficulties. Even under such difficult environment that was rather unexpected and uncontrollable, we could obtain fruitful results on the growth of cherry tree, and found them scientifically significant, especially in terms of biological effects of gravity on earth. Moreover, a lot of interesting interactions of cherry trees with various kinds of animals were observed in parallel to the plant physiology.

Gibberellins/pharmacology↗
NASA NTRS1995

[Quantification and monitoring of vascular resistance in the lower limbs by the Doppler method (animal model)]

The object of this study was to define and validate a non-invasive method of evaluation and monitoring of vascular resistances in the leg. Blood flow velocity was measured by Doppler ultrasound in an animal model (ewe) with similar blood flow characteristics in the lower limb as man and allowing access to the required invasive measurements for validation of the method (pressure and flow). Vascular resistances distal to the measuring point (femoral, for example) were assessed using the resistance index R = D/S, S being the peak systolic deflection and D that of diastolic reflux of the Doppler spectral analysis of flow in the femoral artery. The values and variations of this resistance index were compared with the vascular resistances calculated from measurements of pressure and flow at the point of Doppler sampling and expressed in mmHg/ml/min. Femoral flow was measured by Doppler ultrasound (Doppler-echo), and mean pressure by an arterial catheter introduced into the abdominal aorta. Compression of the lower limb veins induced a venous return resulting in a reduction of cardiac output and femoral flow. During compression, femoral flow decreased by an average of 29% (p < 0.001) although mean pressure and heart rate did not change significantly. The femoral resistance index (Rf) increased by an average of 37.5% (p < 0.01) and vascular resistances increased by 45.9% (p < 0.01). Injection of 1 mg adrenaline induced peripheral vasoconstriction with an increase in blood pressure and a decrease in heart rate and femoral flow.(ABSTRACT TRUNCATED AT 250 WORDS).

Non-NASA Center↗
NASA NTRS1992

System identification of perilymphatic fistula in an animal model

An acute animal model has been developed in the chinchilla for the study of perilymphatic fistulas. Micropunctures were made in three sites to simulate bony, round window, and oval window fistulas. The eye movements in response to pressure applied to the external auditory canal were recorded after micropuncture induction and in preoperative controls. The main pressure stimulus was a pseudorandom binary sequence (PRBS) that rapidly changed between plus and minus 200 mm of water. The PRBS stimulus, with its wide frequency bandwidth, produced responses clearly above the preoperative baseline in 78 percent of the runs. The response was better between 0.5 and 3.3 Hz than it was below 0.5 Hz. The direction of horizontal eye movement was toward the side of the fistula with positive pressure applied in 92 percent of the runs. Vertical eye movements were also observed. The ratio of vertical eye displacement to horizontal eye displacement depended upon the site of the micropuncture induction. Thus, such a ratio measurement may be clinically useful in the noninvasive localization of perilymphatic fistulas in humans.

Non-NASA Center↗
DOE OSTI2020

Evolutionary history, potential intermediate animal host, and cross-species analyses of SARS-CoV-2

To investigate the evolutionary history of the recent outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in China, a total of 70 genomes of virus strains from China and elsewhere with sampling dates between 24 December 2019 and 3 February 2020 were analyzed. To explore the potential intermediate animal host of the SARS-CoV-2 virus, we reanalyzed virome data sets from pangolins and representative SARS-related coronaviruses isolates from bats, with particular attention paid to the spike glycoprotein gene. We performed phylogenetic, split network, transmission network, likelihood-mapping, and comparative analyses of the genomes. Additionally, based on Bayesian time-scaled phylogenetic analysis using the tip-dating method, we estimated the time to the most recent common ancestor and evolutionary rate of SARS-CoV-2, which ranged from 22 to 24 November 2019 and 1.19 to 1.31 × 10 -3 substitutions per site per year, respectively. Our results also revealed that the BetaCoV/bat/Yunnan/RaTG13/2013 virus was more similar to the SARS-CoV-2 virus than the coronavirus obtained from the two pangolin samples (SRR10168377 and SRR10168378). We also identified a unique peptide (PRRA) insertion in the human SARS-CoV-2 virus, which may be involved in the proteolytic cleavage of the spike protein by cellular proteases, and thus could impact host range and transmissibility. Interestingly, the coronavirus carried by pangolins did not have the RRAR motif. Therefore, we concluded that the human SARS-CoV-2 virus, which is responsible for the recent outbreak of COVID-19, did not come directly from pangolins.

59 BASIC BIOLOGICAL SCIENCES↗