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At least 325 records · Page 18

Newborn Screening Quality Assurance Program for CFTR Mutation Detection and Gene Sequencing to Identify Cystic Fibrosis

All newborn screening laboratories in the United States and many worldwide screen for cystic fibrosis. Most laboratories use a second-tier genotyping assay to identify a panel of mutations in the CF transmembrane regulator (CFTR) gene. Centers for Disease Control and Prevention’s Newborn Screening Quality Assurance Program houses a dried blood spot repository of samples containing CFTR mutations to assist newborn screening laboratories and ensure high-quality mutation detection in a high-throughput environment. Recently, CFTR mutation detection has increased in complexity with expanded genotyping panels and gene sequencing. To accommodate the growing quality assurance needs, the repository samples were characterized with several multiplex genotyping methods, Sanger sequencing, and 3 next-generation sequencing assays using a high-throughput, low-concentration DNA extraction method. The samples performed well in all of the assays, providing newborn screening laboratories with a resource for complex CFTR mutation detection and next-generation sequencing as they transition to new methods.

59 BASIC BIOLOGICAL SCIENCES↗

Surface photovoltage in exciton absorption range in CdS

The high resolution, intrinsic spectra of surface photovoltage are reported for semiconducting n-type CdS single crystals. At reduced temperatures (120-160 K) the spectra exhibit three sharp maxima due to A, B and C free exciton transitions. Energy positions of these lines and valence band parameters (spin-orbit and crystal field splittings) estimated from surface photovoltage are in good agreement with values obtained by other methods. The excitonic transitions are very sensitive to surface treatment, i.e. polishing, etching, background illumination and surface doping. The mechanism of direct interaction of free excitons with surface states is proposed to explain exciton lines in surface photovoltage.

Morawski, A.↗

Intercomparison of single-frequency methods for retrieving a vertical rain profile from airborne or spaceborne radar data

This paper briefly reviews several single-frequency rain profiling methods for an airborne or spaceborne radar. The authors describe the different methods from a unified point of view starting from the basic differential equation. This facilitates the comparisons between the methods and also provides a better understanding of the physical and mathematical basis of the methods. The application of several methods to airborne radar data taken during the Convective and Precipitation/Electrification Experiment is shown. Finally, the authors consider a hybrid method that provides a smooth transition between the Hitschfeld-Bordan method, which performs well at low attenuations, and the surface reference method, for which the relative error decreases with increasing path attenuation.

Iguchi, Toshio↗

KAPPA: A PACKAGE FOR SYNTHESIS OF OPTICALLY THIN SPECTRA FOR THE NON-MAXWELLIAN κ-DISTRIBUTIONS BASED ON THE CHIANTI DATABASE

Non-Maxwellian κ-distributions have been detected in the solar transition region and in flares. These distributions are characterized by a high-energy tail and a near-Maxwellian core and are known to have a significant impact on the resulting optically thin spectra arising from collisionally dominated astrophysical plasmas. We developed the KAPPA package for the synthesis of such line and continuum spectra. The package is based on the freely available CHIANTI database and software, and can be used in a similar manner. Ionization and recombination rates together with the ionization equilibria are provided for a range of κ values. Distribution-averaged collision strengths for excitation are obtained using an approximate method for all transitions in all ions available within CHIANTI. The validity of this approximate method is tested through a comparison with direct calculations. Typical precisions of better than 5% are found with all cases being within 10%. Tools for the calculation of synthetic line and continuum intensities are provided and described. Examples of the synthetic spectra and SDO/AIA responses to emission for the κ-distributions are given.

79 ASTRONOMY AND ASTROPHYSICS↗

Improved convolutional coding

Convolutional coding, used to upgrade digital data transmission under adverse signal conditions, has been improved by a method which ensures data transitions, permitting bit synchronizer operation at lower signal levels. Method also increases decoding ability by removing ambiguous condition.

Doland, G. D.↗

Spin–orbit angles of Kepler-13Ab and HAT-P-7b from gravity-darkened transit light curves

Analysis of the transit light curve deformed by the stellar gravity darkening allows us to photometrically measure both components of the spin–orbit angle ψ, its sky projection λ and inclination of the stellar spin axis i{sub ⋆}. In this paper, we apply the method to two transiting hot Jupiter systems monitored with the Kepler spacecraft, Kepler-13A and HAT-P-7. For Kepler-13A, we find i{sub ⋆}=81{sup ∘}±5{sup ∘} and ψ=60{sup ∘}±2{sup ∘} adopting the spectroscopic constraint λ = 58.°6 ± 2.°0 by Johnson et al. In our solution, the discrepancy between the above λ and that previously reported by Barnes et al. is solved by fitting both of the parameters in the quadratic limb-darkening law. We also report the temporal variation in the orbital inclination of Kepler-13Ab, d|cos i{sub orb}|/dt=(−7.0±0.4)×10{sup −6} days{sup −1}, providing further evidence for the spin–orbit precession in this system. By fitting the precession model to the time series of i{sub orb}, λ, and i{sub ⋆} obtained with the gravity-darkened model, we constrain the stellar quadrupole moment J{sub 2}=(6.1±0.3)×10{sup −5} for our new solution, which is several times smaller than J{sub 2}=(1.66±0.08)×10{sup −4} obtained for the previous one. We show that the difference can be observable in the future evolution of λ, thus providing a possibility to test our solution with follow-up observations. The second target, HAT-P-7, is the first F-dwarf star analyzed with the gravity-darkening method. Our analysis points to a nearly pole-on configuration with ψ=101{sup ∘}±2{sup ∘} or 87{sup ∘}±2{sup ∘} and the gravity-darkening exponent β consistent with 0.25. Such an observational constraint on β can be useful for testing the theory of gravity darkening.

79 ASTRONOMY AND ASTROPHYSICS↗

Using Infrasound to Inform Avalanche Hazard Forecasts

Avalanches are natural hazards that occur when an unstable mass of snow breaks away from a mountain slope. It is expected that climate change will lead to increased avalanche activity, which can cause interruptions to water and power infrastructure, transportation blockages, higher risk for loss of life, and changes to ecosystems. Avalanche forecasts are key to mitigating hazards, and observations of recent avalanches comprise one of the key observations for deciding danger level. It is well understood that infrasound can be used to detect and locate snow avalanches in transitional snowpacks, even during snowstorms, but similar studies are lacking for maritime snowpacks. Here we show results from an infrasound field experiment in Tutl’uh (Turnagain Arm), Alaska, USA between January 31 – April 30 field deployment campaign. We show that (1) methods developed for transitional snowpacks can be applied to maritime snowpacks in Alaska, (2) background noise may be higher in this region due to natural and anthropogenic influences, and (3) low-cost infrasound sensors can withstand the harsh Alaskan winter and successfully collect data. We also discuss the impact of this work and a path forward.

58 GEOSCIENCES↗

Optical methods for model angle of attack and transition measurement

A novel laser-based sensor for the in situ measurement of wind tunnel model angle of attack is described and the results of a successful demonstration in the NASA Ames 9 x 7-ft. Supersonic wind tunnel are presented. The design concepts of a scanning laser-based turbulence detector, which will enable nonintrusive, microscopic studies of the onset and extent of transition on wind tunnel test models, is also described.

Owen, F. K.↗

Systems and method for a composite blade with fillet transition

A system includes a turbomachine blade segment including an airfoil with an exterior surface, and a platform coupled to the airfoil having a first side and a second side. The system also includes a concave fillet transition extending between the airfoil and the platform. The concave fillet transition includes one or more interface ply segments extending across the exterior surface of the airfoil and the first or the second side of the platform to form a continuous surface between the airfoil and the platform.

42 ENGINEERING↗

Rapid fitting of particle cascade development data from X-ray film densitometry measurements

A semiautomatic method of fitting transition curves to X-ray film optical density measurements of electromagnetic particle cascades is described. Several hundred singly and multiple interacting cosmic ray events from the JACEE 8 balloon flights were analyzed using this procedure. In addition to greatly increased speed compared to the previous manual method, the semiautomatic method offers increased accuracy through maximum likelihood fitting.

Roberts, E.↗

Transition and mixing in impulsively started jets and vortex rings

A new method is used to analyze transition in impulsively started jets and vortex rings. The method uses similarity variables to reduce the equations for unsteady particle paths to a quasi-autonomous system with the Reynolds number as a parameter. The flow pattern is represented in terms of the phase portrait of this system and the structure of the flow is defined by its critical points. Flow transition is examined in terms of bifurcations in the topology of the phase portrait which occur as the Reynolds number is increased. There is no assumption that the flow is parallel or that it is subjected to small disturbances. The method of analysis, through straightforward, is new and lies outside the usual small disturbance theory used to determine the stability of various profile shapes.

Cantwell, B. J.↗

Treatment of Electronic Energy Level Transition and Ionization Following the Particle-Based Chemistry Model

A new method of treating electronic energy level transitions as well as linking ionization to electronic energy levels is proposed following the particle-based chemistry model of Bird. Although the use of electronic energy levels and ionization reactions in DSMC are not new ideas, the current method of selecting what level to transition to, how to reproduce transition rates, and the linking of the electronic energy levels to ionization are, to the author s knowledge, novel concepts. The resulting equilibrium temperatures are shown to remain constant, and the electronic energy level distributions are shown to reproduce the Boltzmann distribution. The electronic energy level transition rates and ionization rates due to electron impacts are shown to reproduce theoretical and measured rates. The rates due to heavy particle impacts, while not as favorable as the electron impact rates, compare favorably to values from the literature. Thus, these new extensions to the particle-based chemistry model of Bird provide an accurate method for predicting electronic energy level transition and ionization rates in gases.

Liechty, Derek S.↗

Transition research in low-disturbance high-speed wind tunnels

The technical requirements and test data from the Mach 3.5 Pilot Low-Disturbance Tunnel are presented. This unique facility provides a test region with essentially zero-acoustic noise and simulates, for the first time, the low-disturbance conditions of atmospheric flight. Applications to the test results of linear stability theory with the e exp N method indicate that transition locations for both simple and complex flows are well predicted by using N of about 9 to 11.

Beckwith, I. E.↗

Resonant Inelastic X-ray Scattering Calculations of Transition Metal Complexes Within a Simplified Time-Dependent Density Functional Theory Framework

We present a time-dependent density functional theory (TDDFT) approach to compute the light-matter couplings between two different manifolds of excited states relative to a common ground state in the context of 4d transition metal systems. These quantities are the necessary ingredients to solve the Kramers-Heisenberg (KH) equation for resonant inelastic X-ray scattering (RIXS) and several other types of two-photon spectroscopies. The procedure is based on the pseudo-wavefunction approach, where the solutions of a TDDFT calculation can be used to construct excited-state wave- functions, and on the restricted energy window approach, where a manifold of excited states can be rigorously defined based on the energies of the occupied molecular orbitals involved in the excitation process. Thus, the present approach bypasses the need to solve the costly TDDFT quadratic-response equations. We illustrate the applicability of the method to 4d transition metal molecular complexes by calculating the 2p4d RIXS maps of three representative ruthenium complexes and comparing them to experimental results. The method can capture all the experimental features in all three complexes to allow the assignment of the experimental peaks, and relative energies correct to within 0.6 eV at the cost of two independent TDDFT calculations.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

Stopped, Bunched Beams for the TwinSol Facility

Tests of the unitarity of the Cabbibo-Kobayashi-Masakawa (CKM) matrix offer an important avenue for constraining the Standard Model of the electroweak interaction. Several methods are currently used to determine V-ud, the largest element in the top-row normalization test. One such method is through the study of superallowed T =1/2 mixed mirror transitions, which offers a complementary method to the current most-precise value that is determined from superallowed pure Fermi 0(+) 0(+) transitions. The precision currently achievable by this method is currently limited by the very low number of transitions for which the Fermi-to-Gamow-Teller mixing ratio has been measured. St. Benedict, the Superallowed Transition Beta-Neutrino Decay-Ion-Coincidence Trap, is currently under development at the University of Notre Dame's Nuclear Science Laboratory, and intends to determine for a range of new isotopes through measurements of the -neutrino asymmetry parameter a using a linear Paul trap. In order to trap these ions, the fast, continuous secondary beam separated by the TwinSol twin solenoid separator must be thermalized and bunched. The system through which this will be done will feature a large-volume gas cell in which the ions will be thermalized, a double-RF-funnel-based ion guide system for the extraction of the ions, and a radiofrequency quadrupole (RFQ) to provide cooled ion bunches for capture in the Paul trap.

beam cooling↗