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At least 325 records · Page 18

Two-Photon Fluorescence Microscope for Microgravity Research

A two-photon fluorescence microscope has been developed for the study of biophysical phenomena. Two-photon microscopy is a novel form of laser-based scanning microscopy that enables three-dimensional imaging without many of the problems inherent in confocal microscopy. Unlike one-photon optical microscopy, two-photon microscopy utilizes the simultaneous nonlinear absorption of two near-infrared photons. However, the efficiency of two-photon absorption is much lower than that of one-photon absorption, so an ultra-fast pulsed laser source is typically employed. On the other hand, the critical energy threshold for two-photon absorption leads to fluorophore excitation that is intrinsically localized to the focal volume. Consequently, two-photon microscopy enables optical sectioning and confocal performance without the need for a signal-limiting pinhole. In addition, there is a reduction (relative to one-photon optical microscopy) in photon-induced damage because of the longer excitation wavelength. This reduction is especially advantageous for in vivo studies. Relative to confocal microscopy, there is also a reduction in background fluorescence, and, because of a reduction in Rayleigh scattering, there is a 4 increase of penetration depth. The prohibitive cost of a commercial two-photon fluorescence-microscope system, as well as a need for modularity, has led to the construction of a custom-built system (see Figure 1). This system includes a coherent mode-locked titanium: sapphire laser emitting 120-fs-duration pulses at a repetition rate of 80 MHz. The pulsed laser has an average output power of 800 mW and a wavelength tuning range of 700 to 980 nm, enabling the excitation of a variety of targeted fluorophores. The output from the laser is attenuated, spatially filtered, and then directed into a confocal scanning head that has been modified to provide for side entry of the laser beam. The laser output coupler has been replaced with a dichroic filter that reflects the longer-wavelength excitation light and passes the shorter-wavelength fluorescence light. Also, the confocal pinhole has been removed to increase the signal strength. The laser beam is scanned by a twoperpendicular- axis pair of galvanometer mirrors through a pupil transfer lens into the side port of an inverted microscope. Finally, the beam is focused by a 63-magnification, 1.3-numerical- aperture oil-immersion objective lens onto a specimen. The pupil transfer lens serves to match the intermediate image planes of the scanning head and the microscope, and its location is critical. In order to maximize the quality of the image, (that is, the point spread function of the objective lens for all scan positions), the entire system was modeled in optical-design software, and the various free design parameters (the parameters of the spatial-filter components as well as the separations of all of the system components) were determined through an iterative optimization process. A modular design was chosen to facilitate access to the optical train for future fluorescence correlation spectroscopy and fluorescence-lifetime experiments.

Fischer, David G.↗

Passive Microwave Remote Sensing of Soil Moisture

Microwave remote sensing provides a unique capability for direct observation of soil moisture. Remote measurements from space afford the possibility of obtaining frequent, global sampling of soil moisture over a large fraction of the Earth's land surface. Microwave measurements have the benefit of being largely unaffected by cloud cover and variable surface solar illumination, but accurate soil moisture estimates are limited to regions that have either bare soil or low to moderate amounts of vegetation cover. A particular advantage of passive microwave sensors is that in the absence of significant vegetation cover soil moisture is the dominant effect on the received signal. The spatial resolutions of passive Microwave soil moisture sensors currently considered for space operation are in the range 10-20 km. The most useful frequency range for soil moisture sensing is 1-5 GHz. System design considerations include optimum choice of frequencies, polarizations, and scanning configurations, based on trade-offs between requirements for high vegetation penetration capability, freedom from electromagnetic interference, manageable antenna size and complexity, and the requirement that a sufficient number of information channels be available to correct for perturbing geophysical effects. This paper outlines the basic principles of the passive microwave technique for soil moisture sensing, and reviews briefly the status of current retrieval methods. Particularly promising are methods for optimally assimilating passive microwave data into hydrologic models. Further studies are needed to investigate the effects on microwave observations of within-footprint spatial heterogeneity of vegetation cover and subsurface soil characteristics, and to assess the limitations imposed by heterogeneity on the retrievability of large-scale soil moisture information from remote observations.

Njoku, Eni G.↗

Uncertainties in Coastal Ocean Color Products: Impacts of Spatial Sampling

With increasing demands for ocean color (OC) products with improved accuracy and well characterized, per-retrieval uncertainty budgets, it is vital to decompose overall estimated errors into their primary components. Amongst various contributing elements (e.g., instrument calibration, atmospheric correction, inversion algorithms) in the uncertainty of an OC observation, less attention has been paid to uncertainties associated with spatial sampling. In this paper, we simulate MODIS (aboard both Aqua and Terra) and VIIRS OC products using 30 m resolution OC products derived from the Operational Land Imager (OLI) aboard Landsat-8, to examine impacts of spatial sampling on both cross-sensor product intercomparisons and in-situ validations of R(sub rs) products in coastal waters. Various OLI OC products representing different productivity levels and in-water spatial features were scanned for one full orbital-repeat cycle of each ocean color satellite. While some view-angle dependent differences in simulated Aqua-MODIS and VIIRS were observed, the average uncertainties (absolute) in product intercomparisons (due to differences in spatial sampling) at regional scales are found to be 1.8%, 1.9%, 2.4%, 4.3%, 2.7%, 1.8%, and 4% for the R(sub rs)(443), R(sub rs)(482), R(sub rs)(561), R(sub rs)(655), Chla, K(sub d)(482), and b(sub bp)(655) products, respectively. It is also found that, depending on in-water spatial variability and the sensor's footprint size, the errors for an in-situ validation station in coastal areas can reach as high as +/- 18%. We conclude that a) expected biases induced by the spatial sampling in product intercomparisons are mitigated when products are averaged over at least 7 km × 7 km areas, b) VIIRS observations, with improved consistency in cross-track spatial sampling, yield more precise calibration/validation statistics than that of MODIS, and c) use of a single pixel centered on in-situ coastal stations provides an optimal sampling size for validation efforts. These findings will have implications for enhancing our understanding of uncertainties in ocean color retrievals and for planning of future ocean color missions and the associated calibration/validation exercises.

Coastal ocean color↗

Adjoint-Based Algorithms for Adaptation and Design Optimizations on Unstructured Grids

Schemes based on discrete adjoint algorithms present several exciting opportunities for significantly advancing the current state of the art in computational fluid dynamics. Such methods provide an extremely efficient means for obtaining discretely consistent sensitivity information for hundreds of design variables, opening the door to rigorous, automated design optimization of complex aerospace configuration using the Navier-Stokes equation. Moreover, the discrete adjoint formulation provides a mathematically rigorous foundation for mesh adaptation and systematic reduction of spatial discretization error. Error estimates are also an inherent by-product of an adjoint-based approach, valuable information that is virtually non-existent in today's large-scale CFD simulations. An overview of the adjoint-based algorithm work at NASA Langley Research Center is presented, with examples demonstrating the potential impact on complex computational problems related to design optimization as well as mesh adaptation.

Nielsen, Eric J.↗

Space-Based Sensor Web for Earth Science Applications: An Integrated Architecture for Providing Societal Benefits

There is a significant interest in the Earth Science research and user remote sensing community to substantially increase the number of useful observations relative to the current frequency of collection. The obvious reason for such a push is to improve the temporal, spectral, and spatial coverage of the area(s) under investigation. However, there is little analysis available in terms of the benefits, costs and the optimal set of sensors needed to make the necessary observations. Classic observing system solutions may no longer be applicable because of their point design philosophy. Instead, a new intelligent data collection system paradigm employing both reactive and proactive measurement strategies with adaptability to the dynamics of the phenomena should be developed. This is a complex problem that should be carefully studied and balanced across various boundaries including: science, modeling, applications, and technology. Modeling plays a crucial role in making useful predictions about naturally occurring or human-induced phenomena In particular, modeling can serve to mitigate the potentially deleterious impacts a phenomenon may have on human life, property, and the economy. This is especially significant when one is interested in learning about the dynamics of, for example, the spread of forest fires, regional to large-scale air quality issues, the spread of the harmful invasive species, or the atmospheric transport of volcanic plumes and ash. This paper identifies and examines these challenging issues and presents architectural alternatives for an integrated sensor web to provide observing scenarios driving the requisite dynamic spatial, spectral, and temporal characteristics to address these key application areas. A special emphasis is placed on the observing systems and its operational aspects in serving the multiple users and stakeholders in providing societal benefits. We also address how such systems will take advantage of technological advancement in small spacecraft and emerging information technologies, and how sensor web options may be realized and made affordable. Specialized detector subsystems and precision flying techniques may still require substantial innovation, development time and cost: we have presented the considerations for these issues. Finally, data and information gathering and compression techniques are also briefly described.

Habib, Shahid↗

A Simplified Mesh Deformation Method Using Commercial Structural Analysis Software

Mesh deformation in response to redefined or moving aerodynamic surface geometries is a frequently encountered task in many applications. Most existing methods are either mathematically too complex or computationally too expensive for usage in practical design and optimization. We propose a simplified mesh deformation method based on linear elastic finite element analyses that can be easily implemented by using commercially available structural analysis software. Using a prescribed displacement at the mesh boundaries, a simple structural analysis is constructed based on a spatially varying Young s modulus to move the entire mesh in accordance with the surface geometry redefinitions. A variety of surface movements, such as translation, rotation, or incremental surface reshaping that often takes place in an optimization procedure, may be handled by the present method. We describe the numerical formulation and implementation using the NASTRAN software in this paper. The use of commercial software bypasses tedious reimplementation and takes advantage of the computational efficiency offered by the vendor. A two-dimensional airfoil mesh and a three-dimensional aircraft mesh were used as test cases to demonstrate the effectiveness of the proposed method. Euler and Navier-Stokes calculations were performed for the deformed two-dimensional meshes.

Hsu, Su-Yuen↗

Coherent Optical Receiver for PPM Signals under Atmospheric Turbulence

Adaptive combining of experimentally obtained heterodyned pulse position modulated (PPM) signals with pulse-to-pulse coherence in the presence of simulated spatial distortions resembling atmospheric turbulence is demonstrated. The adaptively combined PPM signals are phased up via an LMS algorithm suitably optimized to operate with PPM in the presence of additive shot-noise. A convergence analysis of the algorithm is presented, and results with both, computer simulated and experimentally obtained PPM signals are analyzed.

coherent detection↗

Adaptive Combining of Coherently Detected PPM Signals in the Presence of Atmospheric Turbulence

Adaptive combining of experimentally obtained heterodyned pulse position modulated (PPM) signals with pulse-to-pulse coherence, in the presence of simulated spatial distortions resembling atmospheric turbulence, is demonstrated. The adaptively combined PPM signals are phased up via an LMS algorithm suitably optimized to operate with PPM in the presence of additive shot-noise. A convergence study of the algorithm is presented, and results with both computer simulated and experimentally obtained PPM signals are presented and analyzed.

pulse position modulation (PPM)↗

Streak camera based SLR receiver for two color atmospheric measurements

To realize accurate two-color differential measurements, an image digitizing system with variable spatial resolution was designed, built, and integrated to a photon-counting picosecond streak camera, yielding a temporal scan resolution better than 300 femtosecond/pixel. The streak camera is configured to operate with 3 spatial channels; two of these support green (532 nm) and uv (355 nm) while the third accommodates reference pulses (764 nm) for real-time calibration. Critical parameters affecting differential timing accuracy such as pulse width and shape, number of received photons, streak camera/imaging system nonlinearities, dynamic range, and noise characteristics were investigated to optimize the system for accurate differential delay measurements. The streak camera output image consists of three image fields, each field is 1024 pixels along the time axis and 16 pixels across the spatial axis. Each of the image fields may be independently positioned across the spatial axis. Two of the image fields are used for the two wavelengths used in the experiment; the third window measures the temporal separation of a pair of diode laser pulses which verify the streak camera sweep speed for each data frame. The sum of the 16 pixel intensities across each of the 1024 temporal positions for the three data windows is used to extract the three waveforms. The waveform data is processed using an iterative three-point running average filter (10 to 30 iterations are used) to remove high-frequency structure. The pulse pair separations are determined using the half-max and centroid type analysis. Rigorous experimental verification has demonstrated that this simplified process provides the best measurement accuracy. To calibrate the receiver system sweep, two laser pulses with precisely known temporal separation are scanned along the full length of the sweep axis. The experimental measurements are then modeled using polynomial regression to obtain a best fit to the data. Data aggregation using normal point approach has provided accurate data fitting techniques and is found to be much more convenient than using the full rate single shot data. The systematic errors from this model have been found to be less than 3 ps for normal points.

Varghese, Thomas K.↗

Optimizing spectroheliogram deconvolution methods : MaGIXS - A case study

Over the past five years, new methods to reconstruct spectrally pure maps of the Sun from spectroheliogram data have emerged. In particular, with the rebirth of long-abandoned slitless imaging spectroscopy, one may obtain both spatial and spectral information over a large field of view simultaneously. Yet, depending on the size of the extended source combined with the extent of spectral dispersion, there will be locations in the focal plane where spectral lines from different spatial locations overlap and must be deconvolved. An unfolding method has been successfully developed and demonstrated on the recent rocket flight MaGIXS, which observed several strong emission lines (9 to 30$\AA$) from different portions of two active regions. In the work we are going to present, we conduct a systematic investigation of the parameters that controls and optimizes the inversion method to unfold overlappogram data. We also demonstrate a derived method of the inversion that does not require previous assumptions on the thermal and ionization equilibrium and abundance state of the plasma.

Spectroheliogram↗

Vestibular models for design and evaluation of flight simulator motion

The use of spatial orientation models in the design and evaluation of control systems for motion-base flight simulators is investigated experimentally. The development of a high-fidelity motion drive controller using an optimal control approach based on human vestibular models is described. The formulation and implementation of the optimal washout system are discussed. The effectiveness of the motion washout system was evaluated by studying the response of six motion washout systems to the NASA/AMES Vertical Motion Simulator for a single dash-quick-stop maneuver. The effects of the motion washout system on pilot performance and simulator acceptability are examined. The data reveal that human spatial orientation models are useful for the design and evaluation of flight simulator motion fidelity.

Bussolari, S. R.↗

Satellite Sensed Skin Sea Surface Temperature

Quantitative predictions of spatial and temporal changes the global climate rely heavily on the use of computer models. Unfortunately, such models cannot provide the basis for climate prediction because key physical processes are inadequately treated. Consequently, fine tuning procedures are often used to optimize the fit between model output and observational data and the validation of climate models using observations is essential if model based predictions of climate change are to be treated with any degree of confidence. Satellite Sea Surface Temperature (SST) observations provide high spatial and temporal resolution data which is extremely well suited to the initialization, definition of boundary conditions and, validation of climate models. In the case of coupled ocean-atmosphere models, the SST (or more correctly the 'Skin' SST (SSST)) is a fundamental diagnostic variable to consider in the validation process. Daily global SST maps derived from satellite sensors also provide adequate data for the detection of global patterns of change which, unlike any other SST data set, repeatedly extend into the southern hemisphere extra-tropical regions. Such data are essential to the success of the spatial 'fingerprint' technique, which seeks to establish a north-south asymmetry where warming is suppressed in the high latitude Southern Ocean. Some estimates suggest that there is a greater than 80% chance of directly detecting significant change (97.5 % confidence level) after 10-12 years of consistent global observations of mean sea surface temperature. However, these latter statements should be qualified with the assumption that a negligible drift in the observing system exists and that biases between individual instruments required to derive a long term data set are small. Given that current estimates for the magnitude of global warming of 0.015 K yr(sup -1) - 0.025 K yr(sup -1), satellite SST data sets need to be both accurate and stable if such a warming trend is to be confidently detected. Some of these activities are focussed to develop and deploy instrumentation suitable for the collection of precise in situ measurements of the SSST which can be used to improve the accuracy of satellite measurements, while others develop techniques to generate improved global analyses of sea surface temperature using historical data.

Donlon, Craig↗

Modeling the Absorbing Aerosol Index

We propose a scheme to model the absorbing aerosol index and improve the biomass carbon inventories by optimizing the difference between TOMS aerosol index (AI) and modeled AI with an inverse model. Two absorbing aerosol types are considered, including biomass carbon and mineral dust. A priori biomass carbon source was generated by Liousse et al [1996]. Mineral dust emission is parameterized according to surface wind and soil moisture using the method developed by Ginoux [2000]. In this initial study, the coupled CCM1 and GRANTOUR model was used to determine the aerosol spatial and temporal distribution. With modeled aerosol concentrations and optical properties, we calculate the radiance at the top of the atmosphere at 340 nm and 380 nm with a radiative transfer model. The contrast of radiance at these two wavelengths will be used to calculate AI. Then we compare the modeled AI with TOMS AI. This paper reports our initial modeling for AI and its comparison with TOMS Nimbus 7 AI. For our follow-on project we will model the global AI with aerosol spatial and temporal distribution recomputed from the IMPACT model and DAO GEOS-1 meteorology fields. Then we will build an inverse model, which applies a Bayesian inverse technique to optimize the agreement of between model and observational data. The inverse model will tune the biomass burning source strength to reduce the difference between modelled AI and TOMS AI. Further simulations with a posteriori biomass carbon sources from the inverse model will be carried out. Results will be compared to available observations such as surface concentration and aerosol optical depth.

Penner, Joyce↗

A 3-Channel Algorithm for Retrieving Spatially and Temporally Continuous Cloud Properties Across Different Geostationary Satellite Imagers

Cloud property retrieval algorithms for passive satellite imagers are generally designed to take advantage of all the useful spectral information available for a particular satellite. This strategy optimizes accuracy and reduces misidentification and retrieval biases, particularly for modern satellites with many spectral channels. However, the application of dissimilar algorithms tailored for different satellite sensor scan present a problem within the climate data record (CDR). Algorithm inconsistencies can introduce artificial trends in the CDR that are tied to instrument changes rather than physical changes, especially when older satellites with limited spectral information are included. The NASA CERES (Clouds and the Earth’s Radiant Energy System) data record provides global cloud property retrievals across 23 years and more than 25 satellites. With the goal of producing a spatially and temporally continuous record of cloud properties, the CERES cloud working group has developed algorithms that use only 3 channels that are common to most geostationary satellite imagers: 0.65, 3.9, and 10.8 μm.

Sarah Bedka↗

Convective heating of solid propellant during ignition

A pattern search optimization procedure is used to deduce from test data on the solid rocket boosters of the Space Shuttle the convective heat transfer coefficients between the igniter and main motor combustion gases and the surface of the solid propellant by utilizing a spatial and temporal ignition transient analysis. The 'best' convective coefficients are found which will minimize the overall deviation of the analytical prediction from the ignition transient test data. Computationally, this is achieved by treating the motor in such a way that only four optimization variables must be determined. The resulting coefficients greatly improve the prediction capabilities of the ignition transient analysis for the Space Shuttle SRM.

Murph, J. E.↗

Image-plane processing of visual information

Shannon's theory of information is used to optimize the optical design of sensor-array imaging systems which use neighborhood image-plane signal processing for enhancing edges and compressing dynamic range during image formation. The resultant edge-enhancement, or band-pass-filter, response is found to be very similar to that of human vision. Comparisons of traits in human vision with results from information theory suggest that: (1) Image-plane processing, like preprocessing in human vision, can improve visual information acquisition for pattern recognition when resolving power, sensitivity, and dynamic range are constrained. Improvements include reduced sensitivity to changes in lighter levels, reduced signal dynamic range, reduced data transmission and processing, and reduced aliasing and photosensor noise degradation. (2) Information content can be an appropriate figure of merit for optimizing the optical design of imaging systems when visual information is acquired for pattern recognition. The design trade-offs involve spatial response, sensitivity, and sampling interval.

Huck, F. O.↗

Commercial Applications Multispectral Sensor System

NASA's Office of Commercial Programs is funding a multispectral sensor system to be used in the development of remote sensing applications. The Airborne Terrestrial Applications Sensor (ATLAS) is designed to provide versatility in acquiring spectral and spatial information. The ATLAS system will be a test bed for the development of specifications for airborne and spaceborne remote sensing instrumentation for dedicated applications. This objective requires spectral coverage from the visible through thermal infrared wavelengths, variable spatial resolution from 2-25 meters; high geometric and geo-location accuracy; on-board radiometric calibration; digital recording; and optimized performance for minimized cost, size, and weight. ATLAS is scheduled to be available in 3rd quarter 1992 for acquisition of data for applications such as environmental monitoring, facilities management, geographic information systems data base development, and mineral exploration.

Birk, Ronald J.↗

Effects of Inlet Flow Conditions on Crossflow Jet Mixing

An experimental investigation of the effects of mainstream turbulence, mainstream swirl and non-symmetric mass addition has been conducted for the isothermal mixing of multiple jets injected into a confined rectangular crossflow. Jet penetration and mixing in the near field was studied using planar Mie scattering to measure time-averaged mixture fraction distributions. Orifice configurations were used that were optimized for mixing performance based on previous experimental and computational results for a homogeneous approach flow. Mixing effectiveness, determined using a spatial unmixedness parameter based on the variance of the mean jet concentration distributions, was found to be minimally affected by free-stream turbulence but significantly influenced by the addition of swirl to the mainstream. The results for non-symmetric mass addition indicate that the concentration distribution of the flowfield can be tailored if desired.

Liscinsky, D. S.↗