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At least 325 records · Page 18

Sampling South Pole-Aitken Basin: The Moonrise Approach

The South Pole-Aitken basin (SPA) is the largest of the giant impact basins in the inner Solar System, and its location on Earth s Moon makes it the most accessible. Exploration of SPA through direct collection and analysis of representative materials addresses issues as fundamental as the characteristics of the chemical reservoir from which the Moon originated, early differentiation and production of crust and development of global asymmetry, relationships between magmatic activity and internal thermal evolution, and effects of giant impact events on the terrestrial planets. Owing to its great size and superposition relationships with other lunar impact basins, SPA is the oldest and as such anchors the lunar chronology. Moreover, numerous large impact craters and basins are contained within it such that materials (rocks) of the SPA basin contain a record of the early impact chronology, one less likely to have been affected by the large, late nearside basins (e.g., Imbrium). Understanding the early basin chronology is key to deciphering the sequence and effects of early giant impact bombardment of the inner Solar System. That record exists on the Moon, and materials of the SPA basin will allow us to read that record. Knowledge of the early bombardment history will test - and may reshape - a key paradigm relating to early Solar System evolution. Did the planets form with the alignment of today, or was there a major reorientation of the giant planets that led to destabilization of asteroid orbits, and a cataclysmic bombardment of the inner Solar System hundreds of millions of years after accretion of the planets? Implications include understanding environments for early life-supporting habitats on Earth and Mars, and relationships to new observations of extra-solar planetary systems.

Jolliff, B. L.↗

Decay of the proton-unbound superradiant state in 13 N

Here, the 12 C ( 3 He, 𝑑)⁢ 13 N* reaction is studied in an experiment with a high-resolution magnetic spectrograph, in coincidence with protons detected in silicon detectors near the target. This allows for the observation of angular correlation patterns between the proton transfer and proton decays from populated unbound resonances. A formalism describing the spin polarization of direct reactions is developed to analyze these correlations, and is verified on the known directionally asymmetric decay distributions arising from parity mixing in the 13 N ⁡($\frac{3}{2}$ − , $\frac{5}{2}$ + ) doublet. The same formalism is used to study the decays from the continuum-aligned, broad 3/2 + resonance at 7.9 MeV excitation energy, which arises from superradiant coupling. The observed asymmetric angular correlation patterns are approximately reproduced by adding an “artificial” 3/2 − resonance with strength equal to that of the reaction formalism. This parity-mixing approach serves as a first approximation to a more advanced reaction model of rapid reaction and decay sequences.

13N↗

Craspase is a CRISPR RNA-guided, RNA-activated protease

The CRISPR-Cas type III-E RNA-targeting effector complex gRAMP/Cas7-11 is associated with a caspase-like protein (TPR-CHAT/Csx29) to form Craspase (CRISPR-guided caspase). Here, we use cryo–electron microscopy snapshots of Craspase to explain its target RNA cleavage and protease activation mechanisms. Target-guide pairing extending into the 5' region of the guide RNA displaces a gating loop in gRAMP, which triggers an extensive conformational relay that allosterically aligns the protease catalytic dyad and opens an amino acid side-chain–binding pocket. We further define Csx30 as the endogenous protein substrate that is site-specifically proteolyzed by RNA-activated Craspase. This protease activity is switched off by target RNA cleavage by gRAMP and is not activated by RNA targets containing a matching protospacer flanking sequence. We thus conclude that Craspase is a target RNA–activated protease with self-regulatory capacity.

Science & Technology - Other Topics↗

Chromospheric and transition region structure of the Herbig emission stars HR 5999 and BN Ori

The IUE spectra of HR 5999 and BN Ori were analyzed, showing strong emission lines of C II, C IV, O I, and Si IV (also Mg II in HR 5999), indicating the presence of chromospheres and transition regions around these high mass premain sequence (PMS) stars. Infrared, optical, and ultraviolet observations show that BN Ori has a spectral type of FO-2 IIIe, a bolometric luminosity of 36 L, age 1.5 million yr, and mass 2 to 2.5 solar mass. As HR 5999 fades, the ratio of total to selective absorption increases indicating the appearance of larger grains or changes in grain alignment. Emission measure distributions are used to investigate the atmospheric structure of the stars. As HR 5999 fades the emission measure distribution rises systematically and the inferred transition region pressures increase. The transition region and chromospheric radiative losses are large and imply input mechanical energy fluxes similar to those of lower mass PMS stars.

Brown, A.↗

High-throughput mapping of the phage resistance landscape in E. coli

Bacteriophages (phages) are critical players in the dynamics and function of microbial communities and drive processes as diverse as global biogeochemical cycles and human health. Phages tend to be predators finely tuned to attack specific hosts, even down to the strain level, which in turn defend themselves using an array of mechanisms. However, to date, efforts to rapidly and comprehensively identify bacterial host factors important in phage infection and resistance have yet to be fully realized. Here, we globally map the host genetic determinants involved in resistance to 14 phylogenetically diverse double-stranded DNA phages using two model Escherichia coli strains (K-12 and BL21) with known sequence divergence to demonstrate strain-specific differences. Using genome-wide loss-of-function and gain-of-function genetic technologies, we are able to confirm previously described phage receptors as well as uncover a number of previously unknown host factors that confer resistance to one or more of these phages. We uncover differences in resistance factors that strongly align with the susceptibility of K-12 and BL21 to specific phage. We also identify both phage specific mechanisms, such as the unexpected role of cyclic-di-GMP in host sensitivity to phage N4, and more generic defenses, such as the overproduction of colanic acid capsular polysaccharide that defends against a wide array of phages. Our results indicate that host responses to phages can occur via diverse cellular mechanisms. Our systematic and high-throughput genetic workflow to characterize phage-host interaction determinants can be extended to diverse bacteria to generate datasets that allow predictive models of how phage-mediated selection will shape bacterial phenotype and evolution. The results of this study and future efforts to map the phage resistance landscape will lead to new insights into the coevolution of hosts and their phage, which can ultimately be used to design better phage therapeutic treatments and tools for precision microbiome engineering.

59 BASIC BIOLOGICAL SCIENCES↗

A remote sensing-aided small grains inventory using sequential Landsat imagery

A procedure for manual acreage estimation from Landsat imagery is proposed which involves a stepwise sequence of area stratification, simple size estimation, acreage estimation, and acreage precision calculation. The sample design consists of a systematic point sample of the Landsat imagery for crop type presence calibrated by a double sample of several image points having crop season-coincident ground data. The sample point layout chosen for cost-efficiency in manual sampling is a doubly aligned point matrix. The significant advantage of this sampling approach is that crop information is available from throughout the agricultural reporting unit. For technique demonstration purposes three strata were selected for sampling. The proposed technique can complement more sophisticated computer-based acreage estimation methodologies, and refinements in the sampling techniques promise significant improvements for operational utility.

Thomas, R. W.↗

A model of electronic map interpretation

This paper describes an experiment that provides data for the development of a cognitive model of pilot flight navigation. The model views navigation as a process involving the alignment of mental images with the perceptual view out of the cockpit. The data support a three stage model: (1) the perceptual encoding of the map display, (2) mental rotation of the mental image, and (3) comparison of the image to the environment. The variables that significantly influence the processes embodied in the model in decreasing importance are: speed of processing, display sequencing, map complexity, and rotation angle of the map. The model can be used as a preliminary computational tool in predicting the navigational component of pilot situational awareness.

Aretz, Anthony J.↗

Understanding the Reactions Between Fe and Se Binary Diffusion Couples

Spurred by recent discoveries of high-temperature superconductivity in Fe-Se based materials, the magnetic, electronic, and catalytic properties of iron-chalcogenides have drawn significant attention. Furthermore, much remains to be understood about the sequence of phase formation in these systems. In this work, we shed light on this issue by preparing a series of binary Fe-Se ultrathin diffusion couples via designed thin film precursors and investigating their structural evolution as a function of composition and annealing temperature. Two previously unreported Fe-Se phases crystallized during the deposition process on a nominally room-temperature Si substrate in the 27-33% and 37-47% Fe (atomic percent) composition regimes. Both phases completely decompose after annealing to 200°C in a nitrogen glovebox. At higher temperatures, the sequence of phase formation is governed by Se loss in the annealing process, consistent with what would be expected from the phase diagram. Films rich in Fe (53-59% Fe) crystalized during deposition as β-FeSe (P4/nmm) with preferred c-axis orientation to the amorphous SiO 2 substrate surface, providing a means to non-epitaxial self-assembly of crystallographically aligned, iron-rich β-FeSe for future research. Our findings suggest the crystallization of binary Fe-Se compounds at room temperature via near diffusionless transformations should be a significant consideration in future attempts to prepare metastable ternary and higher order compounds containing Fe and Se.

36 MATERIALS SCIENCE↗

Cusp/cleft auroral activity in relation to solar wind dynamic pressure, interplanetary magnetic field B(sub z) and B(sub y)

Continuous optical observations of cusp/cleft auroral activities within approximately equal to 09-15 MLT and 70-76 deg magnetic latitude are studied in relation to changes in solar wind dynamic pressure and interplanetary magnetic field (IMF) variability. The observed latitudinal movements of the cusp/cleft aurora in response to IMF B(sub z) changes may be explained as an effect of a variable magnetic field intensity in the outer dayside magnetosphere associated with the changing intensity of region 1 field-aligned currents and associated closure currents. Ground magnetic signatures related to such currents were observed in the present case (January 10, 1993). Strong, isolated enhancements in solar wind dynamic pressure (Delta p/p is greater than or equal to 0.5) gave rise to equatorward shifts of the cusp/cleft aurora, characteristic auroral transients, and distinct ground magnetic signatures of enhanced convection at cleft latitudes. A sequence of auroral events of approximately equal to 5-10 min recurrence time, moving eastward along the poleward boundary of the persistent cusp/cleft aurora in the approximately equal to 10-14 MLT sector, during negative IMF B(sub z) and B(sub y) conditions, were found to be correlated with brief pulses in solar wind dynamic pressure (0.1 is less than Delta p/p is less than 0.5). Simultaneous photometer observations from Ny Alesund, Svalbard, and Danmarkshavn, Greenland, show that the events often appeared on the prenoon side (approximately equal to 10-12 MLT), before moving into the postnoon sector in the case we study here, when IMF B(sub y) is less than 0. In other cases, similar auroral event sequences have been observed to move westward in the prenoon sector, during intervals of positive B(sub y). Thus a strong prenoon/postnoon asymmetry of event occurence and motion pattern related to the IMF B(sub y) polarity is observed. We find that this category of auroral event sequence is stimulated bursts of electron precipitation that originate from magnetosheath plasma that has accessed that dayside magnetosphere in the noon or near-noon sector, possibly at high latitudes, partly governed by the IMF orientation as well as by solar wind dynamic pressure pulses.

Sandholt, P. E.↗

CAPG: comprehensive allopolyploid genotyper

Genotyping by sequencing is a powerful tool for investigating genetic variation in plants, but many economically important plants are allopolyploids, where homoeologous similarity obscures the subgenomic origin of reads and confounds allelic and homoeologous SNPs. Recent polyploid genotyping methods use allelic frequencies, rate of heterozygosity, parental cross or other information to resolve read assignment, but good subgenomic references offer the most direct information. The typical strategy aligns reads to the joint reference, performs diploid genotyping within each subgenome, and filters the results, but persistent read misassignment results in an excess of false heterozygous calls. We introduce the Comprehensive Allopolyploid Genotyper (CAPG), which formulates an explicit likelihood to weight read alignments against both subgenomic references and genotype individual allopolyploids from whole-genome resequencing data. We demonstrate CAPG in allotetraploids, where it performs better than Genome Analysis Toolkit’s HaplotypeCaller applied to reads aligned to the combined subgenomic references.

59 BASIC BIOLOGICAL SCIENCES↗

Space station architecture, module, berthing hub, shell assembly, berthing mechanism and utility connection channel

The geometric form of a space station is presented that includes a description of a plurality of modules and berthing hubs, joined by interconnections which are sideways connectable. The modules and hubs are fastened together in a triangular configuration in three dimensions. The interconnections include a pair of opposed, axially aligned, flanged ports and a clamp latch formed from a plurality of sections hinged along their length and extending circumferentially around the flanged ports. A hermetic seal is formed between the ports. A utilities connection channel extends between the ports. The channel has a shell with utilities connectors movable between an extended position to mating connectors in the modules and a withdrawn position. Assembly sequence and common module shell structure is detailed.

Cohen, M. M.↗

Layer-engineered interlayer charge transfer in WSe 2 /WS 2 heterostructures

The layer thickness determines the electronic structure of two-dimensional (2D) materials, leading to different band alignments, which are crucial for the transition metal dichalcogenides heterostructures. Here, we investigated the heterostructure of WSe 2 /WS 2 with different layer thicknesses by steady-state and transient absorption spectroscopy. We observed different ultrafast charge transfer behaviors in 1L-WSe 2 /2L-WS 2 and 2L-WSe 2 /2L-WS 2 few-layer heterostructures. We demonstrate that the layer thickness determines the sequence of intralayer exciton relaxation and interlayer charge transfer. The valley transfer of the band edge induced by the layer thickness can effectively mediate the hot carrier transfer time and interlayer exciton lifetime. Furthermore, these provide us a deeper understanding of carrier dynamics in 2D indirect bandgap semiconductor heterostructures.

77 NANOSCIENCE AND NANOTECHNOLOGY↗

An investigation of the failure modes in U-10Mo monolithic fuel irradiated to high burnup

Here, this study investigated possible failure mode sequences in U-10Mo monolithic fuel irradiated to very-high-burnup. The U-Mo fuel plate used in this study was characterized using scanning electron microscopy (SEM), wavelength dispersive spectroscopy (WDS), and image analysis techniques to investigate how microstructural features may initiate crack formation and propagation through the microstructure, potentially leading to blistering. Distinctly large fission gas pores (FGPs) were observed to preferentially align 5-15µm away from the U-10Mo/Zr interface, parallel to the Zr diffusion barrier. Preferential growth, alignment, and interconnection of FGP could initiate blistering in the fuel plate by creating a fission gas channel, parallel to the U-Mo/Zr interface. FGP alignment in the fuel phase near the U-Mo/Zr interface is believed to be one of the precursors to crack and Type 2 blister formation in monolithic U-Mo fuels. The chemical maps revealed differences in fission product behavior near the U-Mo/Zr interface such that Nd remains in the fuel matrix at the FGP sites. On the other hand, other fission products like Xe and Cs can get trapped in FGPs by Nd in the fuel phase and/or diffuse toward the Zr diffusion barrier.

11 NUCLEAR FUEL CYCLE AND FUEL MATERIALS↗

Maast: genotyping thousands of microbial strains efficiently

Existing single nucleotide polymorphism (SNP) genotyping algorithms do not scale for species with thousands of sequenced strains, nor do they account for conspecific redundancy. Here we present a bioinformatics tool, Maast, which empowers population genetic meta-analysis of microbes at an unrivaled scale. Maast implements a novel algorithm to heuristically identify a minimal set of diverse conspecific genomes, then constructs a reliable SNP panel for each species, and enables rapid and accurate genotyping using a hybrid of whole-genome alignment and k-mer exact matching. We demonstrate Maast’s utility by genotyping thousands of Helicobacter pylori strains and tracking SARS-CoV-2 diversification.

59 BASIC BIOLOGICAL SCIENCES↗

Analysis of the new polarimeter for the Marshall Space Flight Center vector magnetograph

The magnetograph was upgraded in both electronic control of the magnetograph hardware and in the polarization optics. The problems associated with the orignal polarimeter were: (1) field of view errors associated with the natural birefringence of the KD*P crystals; (2.) KD*P electrode failure due to the halfwave dc voltage required in one of the operational sequences; and (3) breakdown of the retardation properties of some KD*Ps when exposed to a zero to halfwave modulation (DC) scheme. The new polarimeter gives up the flexibility provided by two variable waveplates to adjust the retardances of the optics for a particular polarization measurement, but solves the problems associated with the original polarimeter. With the addition of the quartz quarterwave plates, a new optical alignment was developed to allow the remaining KD*P to correct for errors in the waveplates. The new optical alignment of the polarimeter is prescribed. The various sources of error, and how those errors are minimized so that the magnetograph can look at the transverse field in real time are discussed.

West, E. A.↗

An imaging vector magnetograph for the next solar maximum

Researchers describe the conceptual design of a new imaging vector magnetograph currently being constructed at the University of Hawaii. The instrument combines a modest solar telescope with a rotating quarter-wave plate, an acousto-optical tunable prefilter as a blocker for a servo-controlled Fabry-Perot etalon, CCD cameras, and on-line digital image processing. Its high spatial resolution (1/2 arcsec pixel size) over a large field of view (5 by 5 arcmin) will be sufficient to significantly measure, for the first time, the magnetic energy dissipated in major solar flares. Its millisecond tunability and wide spectral range (5000 to 7000 A) enable nearly simultaneous vector magnetic field measurements in the gas-pressure-dominated photosphere and magnetically-dominated chromosphere, as well as effective co-alignment with Solar-A's X ray images. Researchers expect to have the instrument in operation at Mees Solar Observatory (Haleakala) in early 1991. They have chosen to use tunable filters as wavelength-selection elements in order to emphasize the spatial relationships between magnetic field elements, and to permit construction of a compact, efficient instrument. This means that spectral information must be obtained from sequences of images, which can cause line profile distortions due to effects of atmospheric seeing.

Mickey, D. L.↗

Five myofibrillar lesion types in eccentrically challenged, unloaded rat adductor longus muscle--a test model

Sarcomere disruptions are observed in the adductor longus (AL) muscles following voluntary reloading of spaceflown and hindlimb suspension unloaded (HSU) rat, which resemble lesions in eccentrically challenged muscle. We devised and tested an eccentric contraction (ECCON) test system for the 14-day HSU rat AL. Six to 7 hours following ECCON, ALs were fixed to allow immunostaining and electron microscopy (EM). Toluidine blue-stained histology semithin sections were screened for lesion density (#/mm2). Serial semithin sections from the ECCON group were characterized for myosin immunointensity of lesions. Five myofibrillar lesion types were identified in histological semithin sections: focal contractions; wide A-bands; opaque areas; missing A-bands; and hyperstretched sarcomeres. Lesion density by type was greater for ECCON than NonECCON ALs (P< or =0.05; focal contractions and opaque regions). Lesion density (#-of-all-five-types/mm2) was significantly different (ECCON: 23.91+/-10.58 vs. NonECCON: 5.48+/-1.28, P< or =0.05; ECCON vs. SHAM: 0.00+/-0.00; P< or = 0.025). PostECCON optimal tension decreased (Poi-drop, 17.84+/-4.22%) and was correlated to lesion density (R2=0.596), but prestretch tension demonstrated the highest correlation with lesion density (R2=0.994). In lesions, the darkly staining A-band lost the normally organized thick filament alignment to differing degrees across the different lesion types. Ranking the five lesion types by a measure of lesion length deformation (hypercontracted to hyperstretched) at the light microscopy level, related to the severity of thick filament registry loss across the lesion types at the electron microscopic level. This ranking suggested that the five lesion types seen in semithin sections at the light level represented a lesion progression sequence and paralleled myosin immunostaining loss as the distorted A-band filaments spread across the hyperlengthening lesion types. Lesion ultrastructure indicated damage involved calcium homeostasis loss (focal contraction lesions) and "thick-filament-centering" failure of titin (wide A-band lesions) in the early stages of lesion development.

NASA Discipline Musculoskeletal↗

Atmospheric Escape from Magnetized Rocky Exoplanets

Recent Kepler and TESS observations discovered many rocky exoplanets in habitable zones around active main-sequence stars. The upper atmospheres of exoplanets are subject to two important energy sources derived from their host stars. First, the stellar photon flux in the X-ray and XUV bands ionizes and heats the upper atmosphere, driving atmospheric heating, affecting the conductance, and enhancing atmospheric escape. Second, the stellar wind’s interaction with the exoplanet’s intrinsic magnetic field transfers energy to the atmosphere through field aligned currents and Poynting flux. That energy is dissipated in the high latitude cusp and auroral regions through Joule heating which can inflate the atmosphere and also enhance the atmospheric escape rate. This presentation will discuss recent advances in modeling these energy inputs and their consequences for exoplanetary habitability. Also, I will discuss the development of a new model, the (exo) PLANETary Ionosphere-Thermosphere Tool for Research (PLANET-ITTR) and some early results from it.

William Danchi↗