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NASA NTRS1988

Isoelectric focusing analysis of antibody clonotype changes occurring during immune responses using immobilized pH gradients

Serum was collected from rabbits at 2-day intervals following a single injection with tetanus toxoid or at weekly intervals following multiple injections with Micrococcus lysodeikticus cell walls. These sera were analyzed for the presence of individual clonotypes of specific antitetanus or antimicrococcal antibodies by isoelectric focusing in immobilized pH gradients with added carrier ampholytes followed by affinity immunoblotting. The affinity immunoblots obtained clearly defined both the rapid disappearance and late appearance of distinct subsets of antibody clonotypes during the response. These data demonstrate the application of affinity immunoblotting combined with immobilized pH gradients for detecting the subtle changes in specific antibody clonotype patterns which occur during an immune response.

Knisley, Keith A.↗

NASA NTRS1999

Fractal analysis of heart rate dynamics as a predictor of mortality in patients with depressed left ventricular function after acute myocardial infarction. TRACE Investigators. TRAndolapril Cardiac Evaluation

A number of new methods have been recently developed to quantify complex heart rate (HR) dynamics based on nonlinear and fractal analysis, but their value in risk stratification has not been evaluated. This study was designed to determine whether selected new dynamic analysis methods of HR variability predict mortality in patients with depressed left ventricular (LV) function after acute myocardial infarction (AMI). Traditional time- and frequency-domain HR variability indexes along with short-term fractal-like correlation properties of RR intervals (exponent alpha) and power-law scaling (exponent beta) were studied in 159 patients with depressed LV function (ejection fraction <35%) after an AMI. By the end of 4-year follow-up, 72 patients (45%) had died and 87 (55%) were still alive. Short-term scaling exponent alpha (1.07 +/- 0.26 vs 0.90 +/- 0.26, p <0.001) and power-law slope beta (-1.35 +/- 0.23 vs -1.44 +/- 0.25, p <0.05) differed between survivors and those who died, but none of the traditional HR variability measures differed between these groups. Among all analyzed variables, reduced scaling exponent alpha (<0.85) was the best univariable predictor of mortality (relative risk 3.17, 95% confidence interval 1.96 to 5.15, p <0.0001), with positive and negative predictive accuracies of 65% and 86%, respectively. In the multivariable Cox proportional hazards analysis, mortality was independently predicted by the reduced exponent alpha (p <0.001) after adjustment for several clinical variables and LV function. A short-term fractal-like scaling exponent was the most powerful HR variability index in predicting mortality in patients with depressed LV function. Reduction in fractal correlation properties implies more random short-term HR dynamics in patients with increased risk of death after AMI.

Non-NASA Center↗

NASA NTRS1982

Solar rotation measurements at Mount Wilson. III - Meridional flow and limbshift

It is shown that the use of a two-parameter limbshift and a meridional flow velocity fits solar velocity data better than the standard analysis defined by Howard et al. (1980). The data used are the coarse residual velocity arrays, with 34 equal intervals in both sine latitude and sine longitude. There are a total of 2899 full-disk observations between January 1, 1967, and December 12, 1980. The original velocity fields are reconstructed by adding into the residual arrays the large-scale patterns that were measured and removed on a daily basis by a standard reduction. Tests of this reconstituded data set show that no significant errors are introduced in the analysis of large-scale velocity fields. The results of the analysis presented here imply that the study of solar velocity pattern at the level of a few m/s requires that magnetic regions be treated separately from nonmagnetic regions.

Labonte, B. J.↗

NASA NTRS1975

Engineering studies of vectorcardiographs in blood pressure measuring systems, appendix 3

The following subjects were covered: (1) ASM80 manual, (2) signal preprocessing as an aid to on-line EKG analysis, and (3) high speed evaluation of magnetic tape recordings of electrocardiograms. A description of the ASM80 symbolic assembly program for the INTEL 8080 microprocessor and a user's manual were presented. The capability of three redundancy reduction algorithms to produce adequate representations of electrocardiographic data was examined. A hardware device was constructed which carried out zero order interpolation on a signal. Examination of the zero order interpolators reconstructed signal indicated that this representation was adequate for analysis of rhythm. A system to analyze magnetic tapes of electrocardiograms recorded over 24 hour intervals was designed. The recordings are sampled 200 times per second using a Nova computer and a special interface system. This system was tested on several recordings of clinical data, containing over 75 premature ventricular contractions, each one of which was flagged.

Mark, R. G.↗

NASA NTRS1982

Comparison of polar motion data from the 1980 project MERIT short campaign

The short campaign of MERIT (a program of international collaboration to Monitor Earth Rotation and Intercompare the Techniques of observation and analysis) was held during the three-month period August to October, 1980. Based on the analysis of polar motion behavior, the possibility of predicting polar motion for a long time interval (1-2 years in advance) with sufficient accuracy has been found. The predicted polar motion can be used for geodetic purposes. It appears that the accuracy of the prediction is high enough for any practical purpose which requires real time polar motion up to an accuracy of approximately 50 cm. This would include applications such as control of space probes. Attention is given to a MERIT data intercomparison based on raw data, and a MERIT data intercomparison after smoothing.

Mueller, I. I.↗

NASA NTRS1982

GRAVSAT error analysis by means of global spectral analyses of the marine geoid from SEASAT altimeter data

The geographical variability of short wavelength geoid power spectra (geoid roughness), was mapped for the world's oceans between latitudes 72 deg N and 72 deg S. A spectral analysis of SEASAT altimeter data, reduced to sea surface heights, was performed at 2 minute intervals for 15 consecutive days of the 3-day repeat orbit. The geoid roughness represented by these spectra for wavelengths shorter than about 220 km is separated from the total sea height variance and is displayed in the form of a global contour map. The global average geoid roughness is 32 cm RMS, varying from a high in excess of 2 m RMS near deep ocean trenches to a low of 2 cm RMS in the southeast Pacific near the East Pacific Rise. This average value agrees well with previous estimates based on gravimetry and GEOS-3 altimetry. In general, the smoothest areas in the marine geoid overlie relatively young seafloor adjacent mid-ocean spreading centers, where even short-wavelength topographic variations tend to be isostatically compensated.

Source record↗

NASA NTRS1985

Behavior of neutral wind gradients at meteor heights over midlatitude stations

The variation of wind gradients in the altitude range of 80 to 100 km, which contributes information on propagational characteristics of wave phenomena prevailing at those altitudes, was studied. Diurnal and semidiurnal components of the zonal (EW) and meridional (NS) neutral wind data collected over Atlanta using the Georgia Tech Meteor Wind Radar Facility during the period of August 1974 through March of 1978 over the height range of 80 to 100 km are analyzed in detail to obtain information on height gradients in amplitude and phase of neutral wind components over height intervals of 80 to 90 and 90 to 100 km. The details of the data analysis, major results, and conclusions are presented.

Devara, P. C. S.↗

NASA NTRS1987

The velocity distribution of quasar/AGN broad emission line clouds

In the present work, the effects of geometric projection are deconvolved from the observed broad emission line profiles from a number of active galaxies to reveal the intrinsic brightness versus velocity function of the emission-line clouds. Results are presented as total emission per absolute velocity interval, rather than imposing specific dynamical models on the data. It is concluded from the analysis that the preponderance of evidence indicates that the broad-line emission arises from gas with preferred absolute velocity relative to line center.

Puetter, R. C.↗

NASA NTRS1988

High resolution chronology of late Cretaceous-early Tertiary events determined from 21,000 yr orbital-climatic cycles in marine sediments

A number of South Atlantic sites cored by the Deep Sea Drilling Project (DSDP) recovered late Cretaceous and early Tertiary sediments with alternating light-dark, high-low carbonate content. The sedimentary oscillations were turned into time series by digitizing color photographs of core segments at a resolution of about 5 points/cm. Spectral analysis of these records indicates prominent periodicity at 25 to 35 cm in the Cretaceous intervals, and about 15 cm in the early Tertiary sediments. The absolute period of the cycles that is determined from paleomagnetic calibration at two sites is 20,000 to 25,000 yr, and almost certainly corresponds to the period of the earth's precessional cycle. These sequences therefore contain an internal chronometer to measure events across the K/T extinction boundary at this scale of resolution. The orbital metronome was used to address several related questions: the position of the K/T boundary within magnetic chron 29R, the fluxes of biogenic and detrital material to the deep sea immediately before and after the K/T event, the duration of the Sr anomaly, and the level of background climatic variability in the latest Cretaceous time. The carbonate/color cycles that were analyzed contain primary records of ocean carbonate productivity and chemistry, as evidenced by bioturbational mixing of adjacent beds and the weak lithification of the rhythmic sequences. It was concluded that sedimentary sequences that contain orbital cyclicity are capable of providing resolution of dramatic events in earth history with much greater precision than obtainable through radiometric methods. The data show no evidence for a gradual climatic deterioration prior to the K/T extinction event, and argue for a geologically rapid revolution at this horizon.

Herbert, Timothy D.↗

NASA NTRS1989

Global distribution of moisture, evaporation-precipitation, and diabatic heating rates

Global archives were established for ECMWF 12-hour, multilevel analysis beginning 1 January 1985; day and night IR temperatures, and solar incoming and solar absorbed. Routines were written to access these data conveniently from NASA/MSFC MASSTOR facility for diagnostic analysis. Calculations of diabatic heating rates were performed from the ECMWF data using 4-day intervals. Calculations of precipitable water (W) from 1 May 1985 were carried out using the ECMWF data. Because a major operational change on 1 May 1985 had a significant impact on the moisture field, values prior to that date are incompatible with subsequent analyses.

Christy, John R.↗

NASA NTRS1991

An RF interference mitigation methodology with potential applications in scheduling

Software tools for interference analysis and mitigation were developed in the Communications Link Analysis and Simulation System (CLASS) environment for: communications performance evaluation; and mission planning. Potential applications are seen in analysis, evaluation, and optimization of user schedules. Tools producing required separation angles and potential interference intervals can be used as an aid to mutual interference mitigation within a scheduling system.

Wong, Yen F.↗

NASA NTRS1992

Ground-based infrared measurements of carbonyl sulfide total column abundances - Long-term trends and variability

Attention is given to total vertical column abundances of carbonyl sulfide (OCS) derived from time series of high-resolution IR solar absorption spectra recorded near Tucson, Arizona, and in the Swiss Alps. The analysis of both data sets is based on nonlinear least squares spectral fittings of narrow intervals centered on lines of the intense nu3 band of OCS, the P(37) transition at 2045.5788/cm, and the P(15) transition at 2055.8609/cm, with a consistent set of spectroscopic line parameters. The Arizona measurements, recorded on 20 different days between May 1977 and March 1991, show a 10-percent peak-to-peak seasonal cycle with a summer maximum and a winter minimum and a trend in the total column abundance equal to (0.1 +/-0.2) percent/yr, 2sigma. The Alpine total columns exhibit a more complex seasonal variation than noted in the Arizona data. The results from the two sites indicate that there has been no significant change in the OCS total column abundance at northern midlatitudes over the last decade.

Rinsland, C. P.↗

NASA NTRS2001

Treatment and Analysis of a Paint Chip from "Water Lilies": A Fire Damaged Monet

A museum fire in 1958 severely damaged a Monet 'Water Lilies' (1916-1926) painting that was on display. The surface of the painting is very dark with areas of blistering and charring. Over the years, traditional techniques have been found to be ineffective at removal of the soot and char from the surface. The painting, which is now in the care of the New York University (NYU) Conservation Center of the Institute of Fine Arts, was the subject of a study to determine if atomic oxygen treatment could remove the soot and char without damaging the fragile painting underneath. For test purposes, a small chip of paint was removed from the edge of the painting by a conservator at NYU and supplied to NASA Glenn Research Center for atomic oxygen treatment and analysis. The diffuse spectral reflectance, at three locations on the paint chip, was monitored at intervals during the atomic oxygen treatment process. Photo documentation of the chip during treatment was also performed. The color contrast was calculated from the spectral reflectance data as a function of treatment duration. Results of the testing indicated that the contrast improved as a result of the treatment, and the differentiation of colors on the surface was significantly improved. Soot and char could be removed without visibly affecting the gross surface features such as impasto areas. These results indicate the feasibility for the treatment of the 'Water Lilies' painting.

Miller, Sharon K. R.↗

NASA NTRS2016

Survey of the Frequency Dependent Latitudinal Distribution of the Fast Magnetosonic Wave Mode from Van Allen Probes Electric and Magnetic Field Instrument and Integrated Science Waveform Receiver Plasma Wave Analysis

We present a statistical survey of the latitudinal structure of the fast magnetosonic wave mode detected by the Van Allen Probes spanning the time interval of 21 September 2012 to 1 August 2014. We show that statistically, the latitudinal occurrence of the wave frequency (f) normalized by the local proton cyclotron frequency (f(sub cP)) has a distinct funnel-shaped appearance in latitude about the magnetic equator similar to that found in case studies. By comparing the observed E/B ratios with the model E/B ratio, using the observed plasma density and background magnetic field magnitude as input to the model E/B ratio, we show that this mode is consistent with the extra-ordinary (whistler) mode at wave normal angles (theta(sub k)) near 90 deg. Performing polarization analysis on synthetic waveforms composed from a superposition of extra-ordinary mode plane waves with theta(sub k) randomly chosen between 87 and 90 deg, we show that the uncertainty in the derived wave normal is substantially broadened, with a tail extending down to theta(sub k) of 60 deg, suggesting that another approach is necessary to estimate the true distribution of theta(sub k). We find that the histograms of the synthetically derived ellipticities and theta(sub k) are consistent with the observations of ellipticities and theta(sub k) derived using polarization analysis.We make estimates of the median equatorial theta(sub k) by comparing observed and model ray tracing frequency-dependent probability occurrence with latitude and give preliminary frequency dependent estimates of the equatorial theta(sub k) distribution around noon and 4 R(sub E), with the median of approximately 4 to 7 deg from 90 deg at f/f(sub cP) = 2 and dropping to approximately 0.5 deg from 90 deg at f/f(sub cP) = 30. The occurrence of waves in this mode peaks around noon near the equator at all radial distances, and we find that the overall intensity of these waves increases with AE*, similar to findings of other studies.

Boardsen, Scott A.↗

NASA NTRS1998

Mesogranulation as A Distinct Scale of Convection in the Sun

We present evidence for the existence of mesogranulation as a scale of convection distinct from granulation and supergranulation through analysis of full-disk Doppler velocity images of the Sun collected by the Michelson Doppler Imager (MDI) aboard the NASA/ESA Solar and Heliospheric Observatory (SOHO). Our analysis procedures isolate nearly steady flows in the solar photosphere and yield power spectra of convection for spherical harmonic degrees up to I = 1000. Each spectrum exhibits an obvious supergranulation peak at I approximately 130 and a broad secondary peak at I approximately 600 with a distinct break in the spectrum between these peaks at I approximately 300. We believe that this secondary peak is a signature of mesogranulation with typical cell diameters of about 7 Mm. Our standard analysis procedure is to first remove the p-mode oscillation signal by averaging individual Dopplergrams over 17-minute intervals. Next, by fitting to standard functional forms we remove Doppler signals due to the motion of the spacecraft, the convective blueshift, solar rotation including differential rotation, and the meridional circulation in order to produce Dopplergrams dominated by convective motions. By mapping these processed images onto heliographic coordinates and projecting onto spherical harmonics, we produce a power spectrum of solar convection for each 17-minute period. We construct synthetic images and pass them through the same analysis procedure in order to determine the actual solar convection spectrum that reproduces the analyzed results. We find that a small but increasing percentage of high-degree convective power is lost in the analysis as we approach the limit of resolution of the detector but'that the broad, mesogranulation peak at I approximately 600 must be included in the convection spectrum of the synthetic images.

Bachmann, Kurt T.↗

NASA NTRS2014

Challenges in Evaluating Relationships Between Quantitative Data (Carbon Dioxide) and Qualitative Data (Self-Reported Visual Changes)

Understanding the nuances in clinical data is critical in developing a successful data analysis plan. Carbon dioxide (CO2) data are collected on board the International Space Station (ISS) in a continuous stream. Clinical data on ISS are primarily collected via conversations between individual crewmembers and NASA Flight Surgeons during weekly Private Medical Conferences (PMC). Law, et.al, 20141 demonstrated a statistically significant association between weekly average CO2 levels on ISS and self-reported headaches over the reporting period from March 14, 2001 to May 31, 2012. The purpose of this analysis is to describe the evaluation of a possible association between visual changes and CO2 levels on ISS and to discuss challenges in developing an appropriate analysis plan. METHODS & PRELIMINARY RESULTS: A first analysis was conducted following the same study design as the published work on CO2 and self-reported headaches1; substituting self-reported changes in visual acuity in place of self-reported headaches. The analysis demonstrated no statistically significant association between visual impairment characterized by vision symptoms self-reported during PMCs and ISS average CO2 levels over ISS missions. Closer review of the PMC records showed that vision outcomes are not well-documented in terms of clinical severity, timing of onset, or timing of resolution, perhaps due to the incipient nature of vision changes. Vision has been monitored in ISS crewmembers, pre- and post-flight, using standard optometry evaluations. In-flight visual assessments were limited early in the ISS program, primarily consisting of self-perceived changes reported by crewmembers. Recently, on-orbit capabilities have greatly improved. Vision data ranges from self-reported post-flight changes in visual acuity, pre- to postflight changes identified during fundoscopic examination, and in-flight progression measured by advanced on-orbit clinical imaging capabilities at predetermined testing intervals. In contrast, CO2 data are recorded in a continuous stream over time; however, for the initial analysis this data was categorized into weekly averages.

Mendez, C. M.↗

NASA NTRS2018

Discovery of Activities via Statistical Clustering of Fixation Patterns

Human behavior often consists of a series of distinct activities, each characterized by a unique pattern of interaction with the visual environment. This is true even in a restricted domain, such as a piloting an aircraft, where activities with distinct visual signatures might be things like communicating, navigating, and monitoring. We propose a novel analysis method for gaze-tracking data, to perform blind discovery of these hypothetical activities. The method is in some respects similar to recurrence analysis, but here we compare not individual fixations, but groups of fixations aggregated over a fixed time interval. The duration of this interval is a parameter that we will refer to as delta. We assume that the environment has been divided into a set of N different areas-of-interest (AOIs). For a given interval of time of duration delta, we compute the proportion of time spent fixating each AOI, resulting in an N-dimensional vector. These proportions can be converted to integer counts by multiplying by delta divided by the average fixation duration (another parameter that we fix at 280 milliseconds). We compare different intervals by computing the chi-square statistic. The p-value associated with the statistic is the likelihood of observing the data under the hypothesis that the data in the two intervals were generated by a single process with a single set of probabilities governing the fixation of each AOI. The method has been applied to approximately 100 hours of eye movement data collected from pilots in a high-fidelity B747 flight simulator, and the results have been compared to synthetic data in which the each activity is represented as first-order Markov process with random probabilities assigned to the AOIs. Randomly-generated synthetic activities can require thousands of fixations to be discriminated with statistical significance, while the human data can be clustered using averaging windows of some 10's of seconds, suggesting that the actual activities are much more narrowly focused than random Markov models.

activity analysis↗

NASA NTRS1994

Features of steady magnetospheric convection

The large-scale patterns of ionospheric convection and particle precipitation are described during two intervals of steady magnetospheric convection (SMC) on November 24, 1981. The unique data set used in the analysis includes recordings from the worldwide network of magnetometers and all-sky cameras, global auroral images from the Dynamics Explorer (DE) 1 spacecraft, and particle precipitation data from low-altitude National Oceanic and Atmospheric Administration (NOAA) 6 and NOAA 7 spacecraft. The data show that intense magnetospheric convection continued during more than 10 hours under the steady southward interplanetary magnetic field without any distinct substorm signatures. All data sets available confirmed the stable character of the large-scale magnetospheric configuration during this period. In particular, the magnetic flux threading the polar cap was stable (within 10%) during 3.5 hours of continued DE 1 observations. The dayside cusp was located at an unusually low latitude (70 deg CGL). The nightside auroral pattern consisted of two distinct regions. The diffuse aurora in the equatorward half of the expanded (10 deg wide) auroral oval was well-separated from the bright, active auroral forms found in the vicinity of the poleward boundary of the oval. The twin-vortex convection pattern had no signature of the Harang discontinuity; its nightside 'convection throat' was spatially coincident with the poleward active auroras. This region of the auroral oval was identified as the primary site of the short-lived transient activations during the SMC intervals. The energetic particle observations show that the auroral precipitation up to its high-latitude limit is on closed field lines and that particle acceleration up to greater than 30-keV energy starts close to this limit. The isotropic boundaries of the greater than 30-keV protons and electrons were found close to each other, separating regions of discrete and diffuse precipitation. This suggests that these precipitation types originate on the very taillike and very dipolelike field lines, respectively.

Yahnin, A.↗