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MAVEN - Mars Atmosphere and Volatile EvolutioN Mission

NASA's MAVEN mission (to be launched in late 2013) is the first mission to Mars devoted to sampling all of the upper atmosphere neutral and plasma environments, including the well-mixed atmosphere, the exosphere, ionosphere, outer magnetosphere and near-Mars solar wind. It will fill in some measurement gaps remaining from the successful Mars Global Surveyor and the on-going Mars Express missions. The primary science objectives of MAVEN are: 1. Provide a comprehensive picture of the present state of the upper atmosphere and ionosphere of Mars; 2. Understand the processes controlling the present state; and 3. Determine how loss of volatiles to outer space in the present epoch varies with changing solar condition - EUY, solar wind and interplanetary magnetic field measurements will provide the varying solar energy inputs into the system. Knowing how these processes respond to the Sun's energy inputs in the current epoch will provide a framework for projecting atmospheric processes back in time to profile MARS' atmospheric evolution and to explore "where the water went", A description will be given of the science objectives, the instruments, and the current status of the project, emphasizing the value of having collaborations between the MAVEN project and the Mars upper atmosphere science community.

Grebowsky, Joseph M.↗

From Apollo to the Future, the NASA Curation Model for Engaging the Sample Science Community Maximizes Science on Extraterrestrial Samples

The Astromaterials Acquisition and Curation Office at Johnson Space Center (JSC) has enjoyed a long-term partnership (50 years!) with a broad community of planetary sample scientists. This partnership has enabled the curators of planetary samples to plan for and enact evolving requirements for preservation of sample scientific integrity and for handling and long-term storage. The basis for this relationship is a standing peer review advisory committee composed of leading scientists who are recognized for achievements in sample analysis. The committee and its descendants have brought familiarity with the most relevant scientific investigations and the associated analytical and contamination challenges. Beginning with Apollo, the review committee was charged with oversight of curatorial operations and with ensuring fair access to samples. As additional samples from other planetary bodies were acquired, the committee evolved, taking on new responsibilities, reflected in committee name changes. However, oversight of curatorial operations and fair allocation of samples remain basic responsibilities. Committee recommendations are sent to the NASA Headquarters Discipline Scientist for approval. To minimize conflict of interest and maximize fair access, the rules governing the make-up of the committee is structured. Systematic rotation of leadership and staggered terms of membership allow the committee to retain expertise while bringing in fresh ideas. The first peer review committee was called the Lunar Sample Analysis and Planning Team (LSAPT) and was formalized in early 1968 with about 15 members. Their function was to review a) the equipment and procedures used in the new Lunar Receiving Laboratory (LRL); b) the proficiency and capability of the LRL staff; c) the sequence of sample analysis and allocation after quarantine release; and d) the findings of the Preliminary Examination Team (PET). According to LSAPT member Gerald Wasserburg, one of the first issues they faced was deciding whether to have most of the sample analyses performed in house at the LRL or to distribute samples to members of the scientific community. LSAPT concluded that the major scientific investigations should be carried out externally to the LRL by scientists chosen for their expertise in specific disciplines. Further they recommended that the PET's basic characterization of samples be circulated to the broad scientific community. LSAPT set its own agenda, paid attention to facility details, closely monitored the move of samples from the LRL to the interim curatorial facility in 1973, and was active in inspecting curation facilities. Between 1975 and 1979, a Facility Subcommittee of LSAPT oversaw the design and construction of a permanent facility for preservation of lunar samples. The result was an outstanding facility still in use today. In 1977, a separate peer review committee, the Meteorite Working Group (MWG), was formed to evaluate requests for new meteorites then being collected in Antarctica under what would in 1980 become a 3-agency agreement (National Science Foundation, NASA, Smithsonian Institution). By 1979, after lunar samples were moved into the new permanent facility, the vacated gloveboxes and laboratory were prepared for meteorite curation. Recognizing that LSAPT had been helpful in setting up the JSC curatorial facility for Antarctic meteorites, JSC recommended the review committee be given expanded duties, including advice on curation and analysis of materials from other planetary bodies and the name be changed to Lunar and Planetary Sample Team (LAPST). In 1993, LAPST was renamed the Curation and Analysis Planning Team for Extraterrestrial Materials (CAPTEM) to reflect additional functions. CAPTEM is chartered to be (1) a community-based, interdisciplinary forum for discussion and analysis of matters concerning the collection and curation of extraterrestrial samples, including planning future sample return missions and (2) a standing review panel, charged with evaluating proposals requesting allocation of all extraterrestrial samples contained in NASA collections. Efficiency and flexibility are gained through use of subcommittees, both ad hoc and standing. Transition of the MWG to a subcommittee of CAPTEM was completed in 2017. Today subcommittees review allocation requests for lunar samples, Antarctic meteorites, cosmic dust, Stardust cometary samples, Genesis solar wind samples, and samples returned from asteroids. Other subcommittees address facilities, informatics, and micro-cratered substrates. Planetary samples have been sent to research teams in over 30 countries world-wide. The expertise in the care and fair distribution of astromaterials by NASA using this model spans generations of planetary sample scientists and is a valuable resource to be tapped for future sample returns - OSIRIS-REx, Hayabusa 2, and Mars 2020.

Allton, Judith↗

A numerical model of nonmigrating diurnal tides between the surface and 65 km

Observations of surface pressure and middle atmosphere temperatures and winds indicate that a substantial nonmigrating component is present in the diurnal tide. The nonmigrating tides, which propagate with a zonal phase speed that is different from the earth's rotation, are attributed to the diurnal heating of geographically fixed sources. In this study we utilize a classical tidal model to examine the propagation characteristics of diurnal tides. The global fields of tropospheric sensible, radiative, and latent heating used to drive the model are supplied from summer and winter diurnal climatologies of the National Center for Atmospheric Research (NCAR) Community Climate Model (CCM2). A novel aspect of this study is the focus on the relative importance of the nonmigrating components. The classical model successfully reproduces many observed features of the low-latitude diurnal surface pressure tides. In the middle atmosphere, the simulated migrating (or sun-synchronous) tide shows qualitative agreement with November-March Limb Infrared Monitor of the Stratosphere (LIMS) observations. Tropospheric solar heating is clearly the dominant driving force for the migrating tide, with secondary contributions from boundary-layer sensible heating and tropospheric latent heat release. The leading modes of the zonal mean tide are also driven chiefly by tropospheric solar heating. The higher-order modes of the zonal mean and eastward propagating tides may be attributed to the joint effects of tropospheric solar heating, sensible heating, and latent heat release. The LIMS and other data reveal features that cannot be explained or examined within the context of the classical model used in the present study. These include upward phase propagation, vertical attenuation, and temporal variations in the migrating diurnal tide.

Lieberman, Ruth S.↗

Langley Wind Tunnel Data Quality Assurance-Check Standard Results

A framework for statistical evaluation, control and improvement of wind funnel measurement processes is presented The methodology is adapted from elements of the Measurement Assurance Plans developed by the National Bureau of Standards (now the National Institute of Standards and Technology) for standards and calibration laboratories. The present methodology is based on the notions of statistical quality control (SQC) together with check standard testing and a small number of customer repeat-run sets. The results of check standard and customer repeat-run -sets are analyzed using the statistical control chart-methods of Walter A. Shewhart long familiar to the SQC community. Control chart results are presented for. various measurement processes in five facilities at Langley Research Center. The processes include test section calibration, force and moment measurements with a balance, and instrument calibration.

Hemsch, Michael J.↗

ARES Biennial Report 2012 Final

Since the return of the first lunar samples, what is now the Astromaterials Research and Exploration Science (ARES) Directorate has had curatorial responsibility for all NASA-held extraterrestrial materials. Originating during the Apollo Program (1960s), this capability at Johnson Space Center (JSC) included scientists who were responsible for the science planning and training of astronauts for lunar surface activities as well as experts in the analysis and preservation of the precious returned samples. Today, ARES conducts research in basic and applied space and planetary science, and its scientific staff represents a broad diversity of expertise in the physical sciences (physics, chemistry, geology, astronomy), mathematics, and engineering organized into three offices (figure 1): Astromaterials Research (KR), Astromaterials Acquisition and Curation (KT), and Human Exploration Science (KX). Scientists within the Astromaterials Acquisition and Curation Office preserve, protect, document, and distribute samples of the current astromaterials collections. Since the return of the first lunar samples, ARES has been assigned curatorial responsibility for all NASA-held extraterrestrial materials (Apollo lunar samples, Antarctic meteorites - some of which have been confirmed to have originated on the Moon and on Mars - cosmic dust, solar wind samples, comet and interstellar dust particles, and space-exposed hardware). The responsibilities of curation consist not only of the longterm care of the samples, but also the support and planning for future sample collection missions and research and technology to enable new sample types. Curation provides the foundation for research into the samples. The Lunar Sample Facility and other curation clean rooms, the data center, laboratories, and associated instrumentation are unique NASA resources that, together with our staff's fundamental understanding of the entire collection, provide a service to the external research community, which relies on access to the samples. The curation efforts are greatly enhanced by a strong group of planetary scientists who conduct peerreviewed astromaterials research. Astromaterials Research Office scientists conduct peer-reviewed research as Principal or Co-Investigators in planetary science (e. g., cosmochemistry, origins of solar systems, Mars fundamental research, planetary geology and geophysics) and participate as Co-Investigators or Participating Scientists in many of NASA's robotic planetary missions. Since the last report, ARES has achieved several noteworthy milestones, some of which are documented in detail in the sections that follow. Within the Human Exploration Science Office, ARES is a world leader in orbital debris research, modeling and monitoring the debris environment, designing debris shielding, and developing policy to control and mitigate the orbital debris population. ARES has aggressively pursued refinements in knowledge of the debris environment and the hazard it presents to spacecraft. Additionally, the ARES Image Science and Analysis Group has been recognized as world class as a result of the high quality of near-real-time analysis of ascent and on-orbit inspection imagery to identify debris shedding, anomalies, and associated potential damage during Space Shuttle missions. ARES Earth scientists manage and continuously update the database of astronaut photography that is predominantly from Shuttle and ISS missions, but also includes the results of 40 years of human spaceflight. The Crew Earth Observations Web site (http://eol.jsc.nasa.gov/Education/ESS/crew.htm) continues to receive several million hits per month. ARES scientists are also influencing decisions in the development of the next generation of human and robotic spacecraft and missions through laboratory tests on the optical qualities of materials for windows, micrometeoroid/orbital debris shielding technology, and analog activities to assess surface science operations. ARES serves as host to numerous students and visiting scientists as part of the services provided to the research community and conducts a robust education and outreach program. ARES scientists are recognized nationally and internationally by virtue of their success in publishing in peer-reviewed journals and winning competitive research proposals. ARES scientists have won every major award presented by the Meteoritical Society, including the Leonard Medal, the most prestigious award in planetary science and cosmochemistry; the Barringer Medal, recognizing outstanding work in the field of impact cratering; the Nier Prize for outstanding research by a young scientist; and several recipients of the Nininger Meteorite Award. One of our scientists received the Department of Defense (DoD) Joint Meritorious Civilian Service Award (the highest civilian honor given by the DoD). ARES has established numerous partnerships with other NASA Centers, universities, and national laboratories. ARES scientists serve as journal editors, members of advisory panels and review committees, and society officers, and several scientists have been elected as Fellows in their professional societies. This biennial report summarizes a subset of the accomplishments made by each of the ARES offices and highlights participation in ongoing human and robotic missions, development of new missions, and planning for future human and robotic exploration of the solar system beyond low Earth orbit.

Stansbery, Eileen↗

Towards the Development of a Unified Distributed Date System for L1 Spacecraft

The purpose of this grant, 'Towards the Development of a Unified Distributed Data System for L1 Spacecraft', is to take the initial steps towards the development of a data distribution mechanism for making in-situ measurements more easily accessible to the scientific community. Our obligations as subcontractors to this grant are to add our Faraday Cup plasma data to this initial study and to contribute to the design of a general data distribution system. The year 1 objectives of the overall project as stated in the GSFC proposal are: 1) Both the rsync and Perl based data exchange tools will be fully developed and tested in our mixed, Unix, VMS, Windows and Mac OS X data service environment. Based on the performance comparisons, one will be selected and fully deployed. Continuous data exchange between all L1 solar wind monitors initiated. 2) Data version metadata will be agreed upon, fully documented, and deployed on our data sites. 3) The first version of the data description rules, encoded in a XML Schema, will be finalized. 4) Preliminary set of library routines will be collected, documentation standards and formats agreed on, and desirable routines that have not been implemented identified and assigned. 5) ViSBARD test site implemented to independently validate data mirroring procedures. The specific MIT tasks over the duration of this project are the following: a) implement mirroring service for WIND plasma data b) participate in XML Schema development c) contribute toward routine library.

Lazarus, Alan J.↗

Paraglider Recovery Systems

In 1945 it occurred to the writer that if we could discover how to make flexible wings that could be packaged and deployed somewhat like a parachute, such wings would have many new applications as well as replacing some parachutes and rigid wings. Previous uses of flexible materials in aerodynamic surfaces - parachutes, kites, boat sails, and wind mills - were reviewed, and some crude experiments were performed with gliders and kites. Before the end of 1948, the device now generally called a paraglider was evolved and developed sufficiently to merit a patent application. The study was continued privately as time permitted, and in 1954 a short paper on the subject was presented to an audience of about 50 Reserve Air Force officers. This paper was given rather wide distribution, although it suffers from lack of the many kite and glider demonstrations of the original presentation. Little serious interest was shown by the aeronautical community, however, until about a year after Sputnik I. In December 1958 the flexible-wing concept was presented to the Langley Committee on General Aerodynamics with the aid of the hurriedly prepared charts shown in figure 1, faithfully reproduced here for historical purposes.

Flexible wing↗

Global and regional/seasonal color mosaics of Mars

Four regional mosaics of Mars acquired during different seasons, along with their composite as a single global mosaic, have been completed in two colors (red and violet) at scales of 1/16 and 1/64 degrees/pixel. These mosaics were put together from a set of 51 separate mosaics, each acquired from a single Viking orbiter spacecraft orbital revolution. Special techniques were developed and applied to suppress large variations between mosaics introcued by highly variable, optically thin, condensate hazes. The techniques utilize a combination of the spatial characteristics of the hazes (generally broad, low-frequency) along with their modulation of the reginal color ratios (strongly enhancing the violet/red ratios). Photometric-function normalization was applied following the haze removal. Most of the single-orbit mosaics consist of red and violet or red, green, and violet filters, but a few mosaics with only red-filter data were included to fill gaps in global coverage at high northern latitudes. Global coverage is approximately 99 percent complete in red-filter mosaics and approximately 95 percent and approximately 60 percent complete in corresponding violet- and green-filter mosaics, respectively. All of the mosaics are geometrically tied to the 1/256 deg per pixel Mars Digital Image Map (MDIM), which is available on Compact Disk (CD), and which will be used as the base map for Mars Observer data sets. Early in 1993, the single-orbit color mosaics will be distributed to the science community in a six-volume set of CDs. Perhaps the most scientifically interesting parts of this dataset are the overlap regions, which show significant temporal variations in surface and atmospheric features. Surface changes can be categorized as (1) changes that probably occurred during the great dust storms of 1977; (2) changes that occurred soon after 1977 storms due to removal of redistribution of recently deposited dust; (3) changes in the northern lowlands that probably occurred during the dusty southern summer of 1979 (when no great dust storm occurred); and (4) changes associated with strong slope winds in the Tharsis and Elysium regions.

Mcewen, Alfred S.↗

An overview of IACG campaign 4: Solar sources of heliospheric structure observed out of the ecliptic

In 1994, the Inter Agency Consultative Group (IACG), with represenation from ESA, the Japanese Institute of Space and Astronautical Science, the Russian Space Agency, and NASA, initiated a data analysis campaign entitled: 'solar sources of heliospheric structure observed out of the ecliptic' (IACG campaign 4). The primary campaign themes are: the large-scale heliosphere and its dependence on solar photospheric and coronal phenomena; the topology of coronal streamers; the 3D shape of coronal hole boundaries, and solar wind source and acceleration. These themes emphasize the collaborative analysis of remote sensing and in situ data sets for solar features such as coronal holes, coronal mass ejections, and other other solar sources of heliospheric structure. The key observation intervals, covering June 1994 to January 1997, were selected based on the timeline of the unique trajectory of the Ulysses mission. The capabilities and opportunities afforded by the core campaing missions, Ulysses and Yohkoh, and the availability of supportin solar ground observatories and other spacecraft missions, make this campaign of special interest to the solar and heliospheric communities.

Galvin, A. B.↗

Development of a Sodium LIDAR for Spaceborne Missions

We are currently developing laser and electro-optic technologies to remotely measure Sodium (Na) by adapting existing lidar technology with space flight heritage. The developed instrumentation will serve as the core for the planning of a Heliophysics mission targeted to study the composition and dynamics of Earths mesosphere based on a spaceborne lidar that will measure the mesospheric Na layer. There is a pressing need in the Ionosphere Thermosphere - Mesosphere (ITM) community for high-resolution measurements that can characterize small-scale dynamics (i.e. Gravity Waves with wavelengths smaller than a few hundred km) and their effects in the Mesosphere-Lower-Termosphere (MLT) on a global basis. This is compelling because they are believed to be the dominant contributors to momentum transport and deposition in the MLT, which largely drive the global circulation and thermal structure and interactions with the tides and planetary waves in this region. We are developing a spaceborne remote sensing technique that will enable acquisition of global Na density, temperature and wind measurements in the MLT with the spatial and temporal resolution required to resolve issues associated with the structure, chemistry, dynamics, and energetics of this regionA nadir-pointing spaceborne Na Doppler resonance fluorescence LIDAR on board of the ISS will essentially make high-resolution, in time and space, Na density, temperature and vertical wind measurements, from 75-115 km (MLT region). Our instrument concept consisted of a high-energy laser transmitter at 589 nm and highly sensitive photon counting detector that allows for range-resolved atmospheric-sodium-temperature profiles. The atmospheric temperature is deduced from the linewidth of the resonant fluorescence from the atomic sodium vapor D2 line as measured by our tunable laser. We are currently developing a high power energy laser that allows for some day time sodium lidar observations with the help of a narrow bandpass filter based on etalon or atomic sodium Faraday filter with 5 to 10 pm optical bandwidth. The current baseline detector for the lidar instrument is a 16-channel Photomultiplier Tube with receiver electronics that has been space-qualified for the ICESat-2ATLAS mission. Our technique uses the 16-channels as a photon-number-resolving single detector to provide the required full-spectroscopic sodium lineshape waveform for recovering Mesospheric temperature profiles. In this paper, we will describe our instrument concept for a future Heliophysics space mission based on board of the International Space Station (ISS).

lidar↗

Catalog of solar wind events identified from observations by ISTP spacecraft

The ISTP Science Planning and Operations Facility (SPOF), in collaboration with ISTP investigators, is developing a catalog of solar wind events and features. The catalog is primarily based on plasma and magnetic field observations from the WIND and IMP-8 spacecraft. Interplanetary events that may trigger magnetospheric activity are included as well as features of interest for using the solar wind as a plasma laboratory. Catalog coverage begins on September 8, 1992, the start of ISTP science data collection. The catalog is based on Key Parameter data sets (preliminary summary data at approximately 1 min time resolution produced quickly for survey purposes) and as such has limited citability in formal scientific work. Its primary intent is to serve as a reference for identifying candidate periods for further study, such as may be the focus of coordinated data analysis efforts during ISTP and/or IACG Science Campaigns. To facilitate access by members of the ISTP and wider space physics communities, the catalog will be available on the World Wide Web. The contents of the catalog will be described, and samples of catalog information will be presented.

Peredo, M.↗

An Investigation of the Grain Size and Adhesion Strength of High-Speed Impact Ice

Ice accumulation on aircraft from supercooled water droplets (impact icing) can increase drag and decrease lift, leading to a decrease in fuel efficiency and a potential for unsafe situations. Anti-icing materials and coatings are of high interest to prevent aircraft icing. Because no anti-icing material suitable for in-flight situations has been realized, icing models aim to predict the accumulation and shape of the ice to understand its effects. In both cases, understanding the physical mechanism behind ice adhesion and shedding is necessary. A recent literature review has shown that the apparent ice adhesion strength to any specific material varies over several orders of magnitude across studies and test methods. One of the reasons for this high variance is likely to be the varying microstructure of the ice used for different studies. Although the microstructure of impact ice has been studied in the past, those conditions were not relevant to that of in-flight aircraft icing. The microstructure of the ice formed will determine both its bulk and surface properties, yet it has been overlooked in the ice adhesion community. Here, we use optical microscopy to study the non-uniform ice crystal grain size in impact ice accreted in NASA Glenn Research Center’s Icing Research Tunnel at various icing conditions. Similar to other environmental conditions studied in the literature, we show an increase in grain size at larger distances from the growth interface; however, the data shows substantially larger grains than what was expected from the prior literature. Ice accrued at lower temperatures has a smaller grain size at all distances from the growth interface. No change in grain size was noticed when varying only the wind speed. A correlational analysis shows that smaller grain sizes at the growth interface corresponded to an increase in the apparent ice adhesion strength when the adhesive failure was interfacial, but not when the failure was cohesive. These results may explain the overall trend in the literature of increasing apparent adhesion strength with decreasing temperature. Incorporating microstructure trends for various environmental conditions in icing models may increase the certainty in predicting ice adhesion strength and shedding, and will provide a better basis for comparison between adhesive tests on ice produced in different facilities and at different storage times.

• Ice Fabric↗

Monitoring land use and degradation using satellite and airborne data

In July 1990 AVIRIS and AIRSAR data were collected over the Manix Basin Area of the Mojave Desert to study land degradation in an arid area where centerpivot irrigation had been in use. The Manix Basin is located NE of Barstow, California, along Interstate-15 at 34 deg 57 min N 116 deg 35 min W. This region was covered by a series of lakes during the Late Pleistocence and Early Holocene. Beginning in the 1960's, areas were cleared of the native creosote bush-dominated plant community to be used for agricultural purposes. Starting in 1972 fields have been abandoned due to the increased cost of electricity needed to pump the irrigation water, with some fields abandoned as recently as 1988 and 1992. These circumstances provide a time series of abandoned fields which provide the possibility of studying the processes which act on agricultural fields in arid regions when they are abandoned. Ray et al. reported that polarimetric SAR (AIRSAR) could detect that the concentric circular planting furrows plowed on these fields persists for a few years after abandonment and then disappear over time and that wind ripples which form on these fields over time due to wind erosion can be detected with polarimetric radar. Ray et al. used Landsat Thematic Mapper (TM) bandpasses to generate NDVI images of the Manix Basin which showed that the fields abandoned for only a few years had higher NDVI's than the undisturbed desert while the fields abandoned for a longer time had NDVI levels lower than that of the undisturbed desert. The purpose of this study is to use a fusion of a time series of satellite data with airborne data to provide a context for the airborne data. The satellite data time series will additionally help to validate the observation and analysis of time-dependent processes observed in the single AVIRIS image of fields abandoned for different periods of time.

Ray, Terrill W.↗

Review of V/STOL lift/cruise fan technology

This paper presents an overview of supporting technology programs conducted to reduce the risk in the joint NASA/Navy Lift/Cruise Fan Research and Technology Aircraft Program. The aeronautical community has endeavored to combine the low-speed and lifting capabilities of the helicopter with the high-speed capabilities of the jet aircraft; recent developments have indicated a lift/cruise fan propulsion system may provide these desired characteristics. NASA and the Navy have formulated a program that will provide a research and technology aircraft to furnish viability of the lift/cruise fan aircraft through flight experiences and obtain data on designs for future naval and civil V/STOL aircraft. The supporting technology programs discussed include: (1) design studies for operational aircraft, a research and technology aircraft, and associated propulsion systems; (2) wind-tunnel tests of several configurations; (3) propulsion-system thrust vectoring tests; and (4) simulation. These supporting technology programs have indicated that a satisfactory research and technology aircraft program can be accomplished within the current level of technology.

Rolls, L. S.↗

Life in the Clouds of Venus? An Experimental Synthetic Biology Approach

The surface of Venus constitutes the most hellish and biologically inhospitable planetary surface in our solar system, boasting a pH of 0, blistering winds that can melt lead, and pressures of 60 atm. However, during the earlier years of the solar system, without the runaway greenhouse effect that has plagued the planet, Venus potentially housed oceans and perhaps even life. There is a possibility that microbes could have retreated into hospitable niches in the atmosphere, as suggested by Carl Sagan as early as 1967 [1]. For example, 50 km above the raging hell of the Venusian surface, exists a relatively temperate environment that might serve as reservoir for life. This astrobiology project seeks to explore life at the extremes and to theorize whether microbial communities could not only survive but also reproduce in the Venusian atmosphere. Specifically, we ask: are aerosols viable microbial environments? But before we can test for life in the clouds, we have to develop a proper reporter to visualize cell growth in situ. For this purpose, we aimed to develop cell-growth dependent reporters to serve as remote biosensors for cell growth. We developed two using the polA promoter, a DNA-replication dependent promoter, and nrd operon promoter, a cell-cycle dependent promoter. Using these cell-growth reporters, the next step is to aerosolize microbes expressing these reporters in a suspension chamber adapted from a Millikan Drop Apparatus to assay reproduction in an aerosolized environment. Better yet is to test the reproduction of microbes in a microgravity regime such as on ISS.Approach: We engineered two cell-cycle dependent genetic reporters. One was the polA promoter which codes for DNA Polymerase I, a gene active in DNA replication [2]. The other was the nrdP. The activation of ribonucleotide reductase reduces ribonucleotides into deoxyribonucleotides and is involved in the bacterial cell cycle [3]. This promoter began activation during the initiation of DNA replication and is cell-cycle dependent [4]. These promoters were fused to a GFP reporter, transformed into E. coli. The constructs were deposited in the iGEM registry as K847210: Escherichia coli DNA-replication dependent polA promoter K847211: Escherichia coli cell-division dependent nrd promoter Results: Our constructs displayed fluorescence when transformed into NEB-5alpha competent cells. While nrdP-E0840 displayed sufficient fluorescence as verified by fluorescent microscopy, the original polAP-E0840 construct (which uses mut3b GFP) exhibited low expression; while fluorescence was visible under the microscope, the signal was too weak for the camera to recognize. The polA promoter was therefore digest-ed with EcoRI and SpeI then ligated into plasmid pNCS containing a RBS, Clover, and a terminator. Clover is a highly engineered green fluorescent protein that exhibits extreme brightness [5] Fluoresence time course data demonsrated that the genes were induced in a cell cycle dependant manner [6]. Our assays via microscopy and the bulk assay shows that our promoters are functional as cell cycle reporters.Conclusions: The application of such tools are widespread and not limited to astrobiology; nrdP could be used to determine doubling times empirically and could possibly extrapolate DNA content from intensity of signals expressed by polAP. However, we are pri-marily interested in its use in astrobiology.

Escherichia↗

Surface and Flow Field Measurements on the FAITH Hill Model

A series of experimental tests, using both qualitative and quantitative techniques, were conducted to characterize both surface and off-surface flow characteristics of an axisymmetric, modified-cosine-shaped, wall-mounted hill named "FAITH" (Fundamental Aero Investigates The Hill). Two separate models were employed: a 6" high, 18" base diameter machined aluminum model that was used for wind tunnel tests and a smaller scale (2" high, 6" base diameter) sintered nylon version that was used in the water channel facility. Wind tunnel and water channel tests were conducted at mean test section speeds of 165 fps (Reynolds Number based on height = 500,000) and 0.1 fps (Reynolds Number of 1000), respectively. The ratio of model height to boundary later height was approximately 3 for both tests. Qualitative techniques that were employed to characterize the complex flow included surface oil flow visualization for the wind tunnel tests, and dye injection for the water channel tests. Quantitative techniques that were employed to characterize the flow included Cobra Probe to determine point-wise steady and unsteady 3D velocities, Particle Image Velocimetry (PIV) to determine 3D velocities and turbulence statistics along specified planes, Pressure Sensitive Paint (PSP) to determine mean surface pressures, and Fringe Imaging Skin Friction (FISF) to determine surface skin friction (magnitude and direction). This initial report summarizes the experimental set-up, techniques used, data acquired and describes some details of the dataset that is being constructed for use by other researchers, especially the CFD community. Subsequent reports will discuss the data and their interpretation in more detail

Bell, James H.↗

Development of a Weather Robust Microphone Configuration for Sonic Boom Measurements

This paper discusses ongoing development and testing of ground-based, weather-robust microphone measurement systems in conjunction with preparation for community testing of the NASA X-59 quiet supersonic aircraft. Prior efforts [Anderson et al., Proc. Mtgs. Acoust. 42, 040005 (2022)] resulted in the refinement of a ground-plate setup by investigating varied windscreen and plate diameters and thicknesses. The most recent goal has been to make the setup more compact and easier to manufacture. This paper discusses the results of additional tests performed on updated designs meant to find the balance between compactness and performance. Anechoic chamber testing was performed to examine ground plate and windscreen performance and to compare results against prior versions. Outdoor tests included measurements during different wind conditions and over different ground surfaces to examine low-frequency wind noise reduction and ground impedance effects. Results discussed during the presentation suggest the new design strikes an acceptable compromise between compactness, manufacturing ease and robustness, and performance. [Work supported by NASA Langley Research Center through Analytical Mechanics Associates]

sonic boom↗

Indian Ocean warming as key driver of long-term positive trend of Arctic Oscillation

Arctic oscillation (AO), which is the most dominant atmospheric variability in the Northern Hemisphere (NH) during the boreal winter, significantly affects the weather and climate at mid-to-high latitudes in the NH. Although a climate community has focused on a negative trend of AO in recent decades, the significant positive trend of AO over the last 60 years has not yet been thoroughly discussed. By analyzing reanalysis and Atmospheric Model Inter-comparison Project (AMIP) datasets with novel pacemaker experiments, we found that sea surface temperature warming in the Indian Ocean is conducive to the positive trend of AO from the late 1950s. The momentum flux convergence by stationary waves due to the Indian Ocean warming plays an important role in the positive trend of AO, which is characterized by a poleward shift of zonal-mean zonal winds. In addition, the reduced upward propagating wave activity flux over the North Pacific due to Indian Ocean warming also plays a role to strengthen the polar vortex, subsequently, it contributes to the positive trend of AO. Our results imply that the respective warming trend of tropical ocean basins including Indian Ocean, which is either anthropogenic forcing or natural variability or their combined effect, should be considered to correctly project the future AO’s trend.

Indian Ocean↗