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Structural Analysis and Optimization of the Covalent Association between SpyCatcher and a Peptide Tag
Abstract not provided
Structural Analysis and Identification of PhuS as a Heme-Degrading Enzyme from Pseudomonas aeruginosa
Abstract not provided
Structural Analysis of Different LINC Complexes Reveals Distinct Binding Modes
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The electrochemical and local structural analysis of the mesoporous Li[subscript 4]Ti[subscript 5]O[subscript 12] anode
Abstract not provided
Structural analysis of PrBaMn2O5+δ under SOFC anode conditions by in-situ neutron powder diffraction
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Structural analysis of histone deacetylase 8 mutants associated with Cornelia de Lange Syndrome spec
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Structural analysis of RIG-I-like receptors reveals ancient rules of engagement between diverse RNA helicases and TRIM ubiquitin ligases
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Further structural analysis of Cr(III) oligomers in weakly acidic solutions
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Structural Analysis of Bacterial ABC Transporter Inhibition by an Antibody Fragment
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Structural Analysis of the Ash2L/Dpy-30 Complex Reveals a Heterogeneity in H3K4 Methylation
Abstract not provided
Classifying biophysical subpopulations of insulin secretory granules using quantitative whole-cell structure analysis
Pancreatic beta cells contain insulin secretory granules (ISGs), organelles where proinsulin is converted into insulin. As ISGs mature, they undergo extensive biophysical remodeling, producing a spectrum of subpopulations with heterogeneous molecular and spatial characteristics. However, systematic methods to define ISG subpopulations remain underdeveloped. To address this gap in knowledge, we employed soft X-ray tomography (SXT), which can quantitatively measure the biochemical density of ISGs within whole beta cells. Using unsupervised clustering, we classified subpopulations based on molecular density, size, and spatial positioning. Across different insulin secretory stimuli, we observed shifts toward mature and releasable subtypes, demonstrating that exogenous signals can dynamically remodel ISG subpopulation distributions. We extended this methodology to primary beta cells characterized using volume electron microscopy (vEM). Integrating subpopulations from SXT and vEM uncovered insights inaccessible by a single method in isolation. This strategy establishes a framework for defining therapeutic approaches aimed at enriching physiologically beneficial ISG subpopulations.
N,Nʹ-diarylformamidines: Solid state structural analysis and steric-induced stereochemical exchange in solution
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Structural Analysis of Single-Atom Catalysts by X-ray Absorption Spectroscopy
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