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At least 325 records · Page 18

NASA GRC's High Pressure Burner Rig Facility and Materials Test Capabilities

The High Pressure Burner Rig (HPBR) at NASA Glenn Research Center is a high-velocity. pressurized combustion test rig used for high-temperature environmental durability studies of advanced materials and components. The facility burns jet fuel and air in controlled ratios, simulating combustion gas chemistries and temperatures that are realistic to those in gas turbine engines. In addition, the test section is capable of simulating the pressures and gas velocities representative of today's aircraft. The HPBR provides a relatively inexpensive. yet sophisticated means for researchers to study the high-temperature oxidation of advanced materials. The facility has the unique capability of operating under both fuel-lean and fuel-rich gas mixtures. using a fume incinerator to eliminate any harmful byproduct emissions (CO, H2S) of rich-burn operation. Test samples are easily accessible for ongoing inspection and documentation of weight change, thickness, cracking, and other metrics. Temperature measurement is available in the form of both thermocouples and optical pyrometery. and the facility is equipped with quartz windows for observation and video taping. Operating conditions include: (1) 1.0 kg/sec (2.0 lbm/sec) combustion and secondary cooling airflow capability: (2) Equivalence ratios of 0.5- 1.0 (lean) to 1.5-2.0 (rich), with typically 10% H2O vapor pressure: (3) Gas temperatures ranging 700-1650 C (1300-3000 F): (4) Test pressures ranging 4-12 atmospheres: (5) Gas flow velocities ranging 10-30 m/s (50-100) ft/sec.: and (6) Cyclic and steady-state exposure capabilities. The facility has historically been used to test coupon-size materials. including metals and ceramics. However complex-shaped components have also been tested including cylinders, airfoils, and film-cooled end walls. The facility has also been used to develop thin-film temperature measurement sensors.

Robinson, R. Craig↗

Numerical experiments on stability and transition in plane channel flow

Various secondary and tertiary instabilities in plane channael flow are explored via time-dependent numerical simulations using the incompressible Navier-Stokes equations. Comparisons are made between transitional flows at Reynolds numbers 1500, 5000, and 8000. The lambda vortex, detached shear layer, and inverted vortex regions are identified, and the origin of the latter is explained. The laminar breakdown of the Re = 1500 flow is computed with high resolution, and the nature of its ensuing hairpin eddies is clarified by numerical particle paths. The potential of center-mode, rather than wall-mode transitions is proposed, and the resulting flow structure is described.

Zang, Thomas A.↗

Simulations of Boundary-Layer Transition

For incompressible benchmark flows, we have demonstrated the capability of the parabolized stability equations (PSE) to simulate the transition process in excellent agreement with microscopic experiments and direct Navier-Stokes simulations at modest computational cost. Encouraged by these results, we have developed the PSE methodology of three-dimensional boundary-layers in general curvilinear coordinates for the range from low to hypersonic speeds, and for both linear and nonlinear problems. For given initial and boundary conditions, the approach permits simulations from receptivity through linear and secondary instabilities into the late stages of transition where significant changes in skin friction and heat transfer coefficients occur. We have performed transition simulations for a variety of two- and three-dimensional similarity solutions and for realistic flows over swept wings at subsonic and supersonic speeds, the pressure ans suction side of turbine blades at low and medium turbulence levels, and over a blunt cone at Mach number Ma = 8. We present selected results for different transition mechanisms with emphasis on the late stage of transition and the evolution of wall-shear stress and heat transfer.

Herbert, Thorwald↗

An overview of medical-biological radiation hazards in earth orbits

The radiation exposure of crew members on space missions in LEO and GEO is evaluated in a general review. The radiation environment is characterized, taking the trapped radiation belts, solar flares, Galactic and solar cosmic rays, and secondary radiation into account and emphasizing the increased dosages produced by transient phenomena such as solar flares. The biological effects of space radiation in the individual cells, on the body as a whole, and on critical organs are summarized, and the exposure limits and shielding specifications (for spacecraft and for EVA suits) currently used by US mission planners are reviewed. For GEO, 4-g/sq cm Al-equivalent shielding is considered adequate under normal conditions, making EVA in this volatile environment questionable; for anomalously large solar flares, emergency shelter within 10-g/sq cm walls or the capability to retreat to a lower orbit is necessary.

Stauber, M. C.↗

Volatile-rich Crater Interior Deposits in the Polar Regions of Mars: Evidence for Ice Cap Advance and Retreat

Many craters on Mars are partially filled by distinctive material emplaced by post-impact processes. This crater fill material is an interior mound which is generally separated from the walls of the crater by a trough that may be continuous along the crater circumference (i.e. a ring-shaped trough), or which may only partially contact the crater walls (i.e. a crescent-shaped trough). The fill deposit is frequently offset from the crater center and may be asymmetric in plan view. Populations of such craters include those in the circum-south polar cap region, in Arabia Terra, associated with the Medusae Fossae Formation, and in the northern lowlands proximal to the north polar cap. We focus on those craters in circumpolar regions and assess their relationship to polar cap advance and retreat, especially the possibility that fill material represents remnants of a formerly larger contiguous cap. Volatile-rich deposits have the property of being modifiable by the local stability of the solid volatile, which is governed by local energy balance. Here we test the hypothesis that asymmetries in volatile fill shape, profile, and center-location within a crater result from asymmetries in local energy balance within the crater, due mainly to variation of solar insolation and radiative effects of the crater walls over the crater interior. Model profiles of crater fill are compared with MOLA topographic profiles to assess this hypothesis. If asymmetry in morphology and location of crater fill are consistent with radiative-dominated asymmetries in energy budget within the crater, then 1) the volatile-rich composition of the fill is supported (this process should not be effective at shaping volcanic or sedimentary deposits), and 2) the dominant factor determining the observed shape of volatile-rich crater fill is the local radiative energy budget (and erosive processes such as eolian deflation are secondary or unnecessary). We also use a geographic and energy model approach to specifically test the idea that material in partially filled craters around the south pole may once have been contiguous to the cap and may have been sustained and modified by radiative processes specific to the crater environment (as opposed to the surrounding plains) as the cap retreated.

Russell, Patrick S.↗

Development of a Novel Space Flight Plan to Monitor Female Mice Fertility Using Reduced Crew Time

Ovarian estrogen impacts the normal homeostatic and metabolic processes of all tissues and organ systems within the body: particularly, but not limited to canonical space-flight impacted systems: bone, muscle, immune, wound repair, and cardiovascular. Effects of space flight on the ovarian estrogen production are therefore critical to our understanding of all space flight experiments using female mice, the current paradigm being used on the International Space Station (ISS). Recently, we demonstrated that vaginal wall histology could be used to determine the stage of the estrous cycle in female mice at the time of sacrifice in space. Moreover, this robust technique was completed following two post-flight freezethaw procedures of the carcasses (RR1 experiment). Thus, this technique represents a viable mechanism to determine the estrous cycle status of the female at the time of sacrifice and can be completed in a manner that does not impact primary experimental objectives. We propose that vaginal wall histology become a standard procedure completed on all mice sacrificed in space and that the individual estrous status of each animal be shared with all investigators. While evidence of estrous cyclicity was present in long-term (33 day) RR1 mice, fertility of female mice exposed to weightlessness remains unknown. In preparation for an upcoming funded NASA flight investigating the effects of long duration spaceflight on female fertility, we have refined our experimental design to minimize crew flight time and to accommodate the duration of Dragon capsule berth. These refinements maintain all our proposed primary and secondary experimental objectives. Briefly, in order to evaluate fertility, we will super ovulate mice using standard procedures (PMSG hCG), followed by collection of reproductive tract after follicular stimulation alone (PMSG) or following ovulation (hCG). Ovarian folliculogenesis and ovulation rate will be determined in fixed tissues following return in order to determine fertility. Ovarian and uterine tissues will also be evaluated by hormonal and gene expression profiling using quantitative approaches (radioimmunoassays, western blots, digital droplet PCR). Comparisons will be made to contemporary vivarium and Rodent Research Hardware Transporter and Habitat housed animals maintained on earth. Supported by NNX15AB48G to JST.

Christenson, Lane↗

CFD analysis on control of secondary losses in STME LOX turbines with endwall fences

The rotor blade in the newly designed LOX turbine for the future Space Transportation Main Engine (STME) has a severe flow turning angle, nearly 160 degrees. The estimated secondary loss in the rotor alone accounts for nearly 50 percent of the total loss over the entire stage. To reduce such a loss, one of the potential methods is to use fences attached on the turbine endwall (hub). As a prelude to examining the effects of endwall fence with actual STME turbine configuration, the present study focuses on similar issues with a different, but more generic, geometry - a rectangular duct with a 160-degree bend. The duct cross-section has a 2-to-1 aspect ratio and the radii of curvature for the inner and outer wall are 0.25 and 1.25 times the duct width, respectively. The present emphasis lies in examining the effects of various fence-length extending along the streamwise direction. The flowfield is numerically simulated using the FDNS code developed earlier by Wang and Chen. The FDNS code is a pressure based, finite-difference, Navier-Stokes equations solver.

Chyu, Mingking K.↗

Sidewall Effects on Exact Reynolds-Stress Budgets in an Impinging Shock Wave/Boundary Layer Interaction

Large-eddy simulations are performed using wall-resolved mesh for a Mach 2.29 impinging shock wave/boundary-layer interaction. Flow conditions are based on an experiment and therefore entire span was simulated, including the two sidewalls. Mean flow comparison with the experimental data showed that the interaction was larger in the simulation. Time-series analysis of a rake of pressure probes immediately downstream of the mean reflected shock position showed a peak in weighted power spectral density occurred about $St_{Lint}=0.01$, owing to a larger interaction length. Budgets of Reynolds-stress transport calculated across the span and along the corner bisector showed high degree of anisotropy. Merging of the secondary flows and separation along the corner gives rise to unstablecounter rotating vortices, which straddle the corner and grow in size. This also leads to a development of new behavior in the viscous sublayer along the corner bisector, where the pressure strain andmolecular diffusion mechanisms become prominent.

Vyas, Manan A.↗

Missile impact craters (White Sands Missile Range, New Mexico) and applications to lunar research: Contributions to astrogeology

Craters in natural materials at White Sands Missile Range, N. Mex., were produced by the impact of high-velocity to hypervelocity missiles traveling along oblique trajectories with kinetic energies between 2.1 and 81 × 1014 ergs. The oblique impacts produce craters 2 to 10 m across with morphologies and ejecta that are bilaterally symmetrical with respect to the plane of the missile trajectory. Rims are high and the amount of ejecta large in down-trajectory and lateral directions, whereas rims are low to nonexistent and ejecta thin to absent up-trajectory. Symmetry development and modifications of the symmetry are a function of target material, local topography, and angle of impact. Seven mappable units can be recognized in and around the craters. Three of these are ejecta: thick ejecta near the crater, thin to discontinuous ejecta at greater distances, and scattered ejecta at the greatest distances to the limit of throwout. These ejecta units may be absent on the up-trajectory side; if present, they are rarely as thick or continuous as on other sides of the crater. Three units are target materials: undeformed target material exposed in local patches through thin to discontinuous ejecta and everywhere between the fragments of scattered ejecta, tilted and broken target material exposed in upper crater walls, and shattered and fractured target material exposed on the up-trajectory crater wall. The seventh unit is slope material composed of talus and fallback within the crater. Development, character, and exposure of these units varies chiefly with the target material. Ejecta from the craters is chiefly broken but relatively undeformed target material that may range in size from very fine grained debris to large blocks. Where the target is porous, significant amounts of the ejecta are composed of sheared and compressed fragments, some coated with dark layers of mixed projectile pieces, powder, and fused metal mixed with crushed target material. For layered targets, the original stratigraphic sequence is crudely preserved and in inverted order in thick ejecta. Secondary impact craters are produced by the impact of ejected fragments when the surrounding surface materials are sufficiently weak. A wide variety of secondary impact crater relations may result. Secondary craters nearest the primary crater have blocks in them that are larger than or the same size as the crater they produced. Farther from the primary crater, the fragments are generally smaller than the secondary crater and are ejected from it. Excavation of four craters revealed a mixed breccia beneath the crater floor composed of missile pieces, sheared and compressed target material, and crushed debris. Banded, disaggregated target material and nonmixed breccia surrounded the mixed breccia, and these breccias were surrounded by a zone of conjugate fractures. Beneath the ejecta on the lateral and down-trajectory crater flanks, the target materials were tilted upward and broken. Up-trajectory, open fractures and downward displacement occurred in two of the craters. No displacement was observed for the other two. Beneath the down-trajectory rims of craters with distinct layering, overturned synclines were observed. Missile breakup and behavior during cratering are a function of target and missile properties. Missile breakup depends on missile velocity and is most extensive at high velocities, where the missile is fragmented, powdered, and partly fused. Burial of missile or its fragmented, powdered, and fused remains is greatest for porous targets and least for dense cohesive targets. For very porous targets, camouflet structures containing the fragmented missile may form. Least squares fit to the data on craters in dry to moist targets indicate V(a) = 10^(-11.433)E(p)^(1.205) where V(a) is the volume of the apparent crater and E(p) is the kinetic energy of the missile. This equation is consistent with expectations of the equations relating apparent depth and radius to kinetic energy. Extrapolation of displaced masses and kinetic energies for laboratory impacts with sand and rock converge near 10^(15) to 10^(16) ergs, where the extrapolations are near the data on missile impact craters, corrected for impact angle. Displaced masses of craters produced by missile impacts and by chemical explosives with small scaled depths of burial are about the same when the kinetic energies of the missiles (corrected for angle of impact) are equal to the TNT equivalent energy of the explosive. The problem of equivalent scaled depth of burst for an impact crater is complicated and not entirely resolved, however. Both missile impact craters and chemical explosive craters in water-saturated targets are larger than their counterparts in dry to moist materials. Data collected during the study of missile impact craters have helped resolve a number of problems in lunar research: (1) the soillike nature of lunar surface materials was predicted, (2) sizes of craters produced by artificial impacts were correctly predicted, (3) certain features imaged by Surveyor were found to be analogous to features associated with missile impact craters, {4) missile impacts were used in support of the Apollo passive seismic experiment, (5) craters seen in Apollo orbital photographs were found to be similar to some missile impact craters, (6) missile impact craters supplied data on sample collection and crater phenomenology used in training astronauts, and (7) some returned lunar samples are similar to coated, sheared, and compressed fragments ejected from missile impact craters.

H. J. Moore↗

Small experiments for the maturation of orbital cryogenic transfer technologies

The no-vent method is a promising approach to handling the problems of low-g venting during propellant transfer. A receiver tank is first cooled to remove thermal energy from the tank wall and the resultant vapor vented overboard. The nozzles mix the incoming liquid and residual vapor in the tank maintaining a thermodynamic state which allows the tank to fill with liquid without venting. Ground based testing at NASA Lewis Research Center (LeRC) has demonstrated the no-vent fill process and attempted to bound its low-gravity performance. But, low-gravity testing is required to validate the method. As an alternative to using a dedicated spacecraft for validation, several small scale experiments to study no-vent fill in low-g were formulated. Cost goals quickly limited the search to two possibilities: a secondary payload on the space shuttle, or a small scale sounding rocket experiment. The key issues of small scale experimentation are discussed, and a conceptual design of a sounding rocket experiment with liquid hydrogen for studying the fill process is presented.

Chato, David J.↗

Revisiting the Field Geology of Taurus-Littrow

Integration of Apollo 17 field observations and photographs, sample investigations, Lunar Reconnaissance Orbiter Camera images, Chandrayaan-1 Moon Mineralogy Mapper (M(sup 3)) spectra, and Miniature Radio Frequency (Mini-RF) S-band radar images provides new insights into the geology of the valley of Taurus-Littrow on the Moon. Connecting the various remote observations to sample data enables a set of new conclusions to be drawn regarding the geological evolution of the valley. Structural considerations and published and recalculated Ar-40/Ar-39 analyses of samples from the North Massif and the Sculptured Hills indicate that the Crisium basin formed about 3.93 Ga; the Serenitatis basin about 3.82 Ga; and the Imbrium basin no earlier than 3.82 Ga and no later than the average of 3.72 Ga for 33 age dates from samples of the valley's mare basalts. Strong evidence continues to support the conclusion of others (Lucchitta, 1972; Spudis et al., 2011; Fassett et al., 2012) that the Sculptured Hills physiographic unit consists of Imbrium ejecta. Interpretation of M(sup 3) spectral data and Apollo 17 samples indicate that rock units of the Sculptured Hills consist of a largely coherent, Mg-suite pluton. LROC NAC stereo images and Mini-RF data indicate the presence of several exposed pyroclastic fissures across the Sculptured Hills. Rim boulders at Camelot Crater constitute nearly in situ wall rocks of that crater rather than ejecta and provide an opportunity for investigations of remanent magnetic field orientation at the time of the eruption of late mare basalt lavas in the valley. Paleomagnetic field orientation information also may be obtained relative to melt-breccia contacts in North Massif boulders that suggest original horizontal orientations. LROC images indicate the existence of two temporally separate light mantle avalanche deposits. The origin, potential flow mechanisms, and geology of the youngest avalanche from the South Massif have been clarified. The existence of two distinct light mantle avalanches raises doubt about the association of either light mantle avalanche with secondary impacts related to the Tycho impact event. Alternatively, the Lee-Lincoln thrust fault appears to have triggered the second light mantle avalanche between 70 and 110 Ma. A simple structural analysis shows that this thrust fault dips 20-25 degrees to the southwest where it crosses the North Massif and to the west where it crosses the valley floor. Mini-RF data reveal a line of reduced reflections roughly perpendicular to contours on the North Massif about 3 km to the east of the Lee-Lincoln fault. Although this line is possibly an older ancillary fault, LROC NAC stereo images indicate that it may be best explained as a pyroclastic fissure. A debris flow of dark, apparent pyroclastic ash lies below the southeast end of the potential fissure. Finally, young lunar impact glass sample 70019 has been precisely located within LROC NAC images and oriented for the first time using 60 mm (f.l.) sample documentation photographs. Sample 70019 can now be employed in lunar paleomagnetic field orientation studies.

Schmitt, H. H.↗

Multiple incidence angle SIR-B experiment over Argentina

The Shuttle Imaging Radar (SIR-B), the second synthetic aperture radar (SAR) to fly aboard a shuttle, was launched on October 5, 1984. One of the primary goals of the SIR-B experiment was to use multiple incidence angle radar images to distinguish different terrain types through the use of their characteristic backscatter curves. This goal was accomplished in several locations including the Chubut Province of southern Argentina. Four descending image acquisitions were collected providing a multiple incidence angle image set. The data were first used to assess stereo-radargrammetric techniques. A digital elevation model was produced using the optimum pair of multiple incidence angle images. This model was then used to determine the local incidence angle of each picture element to generate curves of relative brightness vs. incidence angle. Secondary image products were also generated using the multi-angle data. The results of this work indicate that: (1) various forest species and various structures of a single species may be discriminated using multiple incidence angle radar imagery, and (2) it is essential to consider the variation in backscatter due to a variable incidence angle when analyzing and comparing data collected at varying frequencies and polarizations.

Cimino, Jobea↗

Small experiments for the maturation of orbital cryogenic transfer technologies

The no-vent fill method is a promising approach to handle the problems of low-g venting during propellant transfer. A receiver tank is first cooled to remove thermal energy from the tank wall and the resultant vapor vented overboard. Then nozzles mix the incoming liquid and residual vapor in the tank maintaining a thermodynamic state which allows the tank to fill with liquid without venting. Ground based testing at NASA Lewis Research Center (LeRC) has demonstrated the no-vent fill process and attempted to bound its low-gravity performance. But, low-gravity testing is required to validate the method. As an alternative to using a dedicated spacecraft for validation the authors have formulated several small scale experiments to study no-vent fill in low-g. Cost goals quickly limited the search to two possibilities: a secondary payload on the Space Shuttle, or a small scale sounding rocket experiment. This paper will discuss the key issues of small scale experimentation and present a conceptual design of a sounding rocket experiment with liquid hydrogen for studying the fill process.

Chato, David J.↗

Supercritical Water Oxidation (SCWO) Trade Study and 2021 Final Report

SCWO is a promising technology whose main benefits is that it is capable of completely mineralizing organic compounds in wastewater and separating inorganic salts. This means that SCWO can theoretically function as a single step water treatment system. It performs the functions of both the primary treatment system and the secondary brine drying function to achieve near 100% water recovery. It can also handle solids so no pretreatment of the feed is required. A preliminary trade study indicates that SCWO could be competitive with the ISS state of the art if its power consumption could be reduced. Thermal and mechanical energy recovery system exist that could reduce SCWO energy consumption to a level that is competitive with the state of the art. In addition, increasing the feed wastewaters organic content by including other wastes, such as feces, could produce a reactor that is thermally self-sustaining. There are 5 different types of SCWO reactors. They are; tubular, tank, impingement, transpiring wall reactors (TWR) and super critical water mixing (SCWM) reactors. Of these the best suited for NASA missions are the impingement and SCWM reactors. The impingement reactor is best suited for near term missions because it can treat the low organic content wastewaters such as are expected for initial Lunar and Mars missions and has the ability to remove precipitated inorganic solids. The SCWM reactor is best suited for longer duration CELSS type missions where high organic content wastewater is available and will resolve solids fouling and corrosion issues. In addition, these two reactors have a long NASA heritage and have been developed specifically for space flight application. Tubular reactors suffer from plugging issues which is a safety concern. Tank reactors will increase in size inversely with gravity, which will trade poorly in Lunar and Mars environments where gravity is reduced. TWR reactors require high organic content wastewater feed and the SCWM reactor is an optimized version of the TWR for NASA applications. It is recommended that in FY 2022 NASA continue to fully evaluate the ability of SCWO to produce potable water and remove inorganics as a solid byproduct. This should be done using the impingement reactor for near term missions and SCWM for longer duration missions. NASA should also develop optimized recuperative heat exchangers and pressure recovery devices to reduce power consumption and investigate inclusion of feces and other organic wastes into the wastewater model to increase its organic content and further reduce thermal energy requirements.

Supercritical Water Oxidation↗

Electroconductive PET/SWNT Films by Solution Casting

The market for electrically conductive polymers is rapidly growing, and an emerging pathway for attaining these materials is via polymer-carbon nanotube (CNT) nanocomposites, because of the superior properties of CNTs. Due to their excellent electrical properties and anisotropic magnetic susceptibility, we expect CNTs could be easily aligned to maximize their effectiveness in imparting electrical conductivity to the polymer matrix. Single-walled carbon nanotubes (SWNT) were dispersed in a polyethylene terephthalate (PET) matrix by solution blending then cast onto a glass substrate to create thin, flexible films. Various SWNT loading concentrations were implemented (0.5, 1.0, and 3.0 wt.%) to study the effect of additive density. The processing method was repeated to produce films in the presence of magnetic fields (3 and 9.4 Tesla). The SWNTs showed a high susceptibility to the magnetic field and were effectively aligned in the PET matrix. The alignment was characterized with Raman spectroscopy. Impedance spectroscopy was utilized to study the electrical behavior of the films. Concentration and dispersion seemed to play very important roles in improving electrical conductivity, while alignment played a secondary and less significant role. The most interesting result proved to be the effect of a magnetic field during processing. It appears that a magnetic field may improve dispersion of unmodified SWNTs, which seems to be more important than alignment. It was concluded that SWNTs offer a good option as conductive, nucleating filler for electroconductive polymer applications, and the utilization of a magnetic field may prove to be a novel method for CNT dispersion that could lead to improved nanocomposite materials.

Steinert, Brian W.↗

A Three-Dimensional CFD Investigation of Secondary Flow in an Accelerating, 90 deg Elbow

NASA Glenn Research Center has recently applied the WIND National Code flow solver to an accelerating elbow with a 90 deg. bend to reveal aspects of secondary flow. This elbow was designed by NACA in the early 1950's such that flow separation would be avoided. Experimental testing was also done at that time. The current three dimensional CFD investigation shows that separation has indeed been avoided. Using its three-dimensional capability, this investigation provides various viewpoints in several planes that display the inception, development, and final location of a passage vortex. Its shape first becomes discernible as a vortex near the exit of the bend. This rendition of the exit passage vortex compares well with that found in the experiments. The viewpoints show that the passage vortex settles on the suction surface at the exit about one-third of the distance between the plane wall and midspan. Furthermore, it projects into the mainstream to about one-third of the channel width. Of several turbulence models used in this investigation, the Spalart Alimaras, Baldwin Lomax, and SST (Shear Stress Transport) models were by far the most successful in matching the experiments.

Cavicchi, Richard H.↗

Analysis of xylem formation in pine by cDNA sequencing

Secondary xylem (wood) formation is likely to involve some genes expressed rarely or not at all in herbaceous plants. Moreover, environmental and developmental stimuli influence secondary xylem differentiation, producing morphological and chemical changes in wood. To increase our understanding of xylem formation, and to provide material for comparative analysis of gymnosperm and angiosperm sequences, ESTs were obtained from immature xylem of loblolly pine (Pinus taeda L.). A total of 1,097 single-pass sequences were obtained from 5' ends of cDNAs made from gravistimulated tissue from bent trees. Cluster analysis detected 107 groups of similar sequences, ranging in size from 2 to 20 sequences. A total of 361 sequences fell into these groups, whereas 736 sequences were unique. About 55% of the pine EST sequences show similarity to previously described sequences in public databases. About 10% of the recognized genes encode factors involved in cell wall formation. Sequences similar to cell wall proteins, most known lignin biosynthetic enzymes, and several enzymes of carbohydrate metabolism were found. A number of putative regulatory proteins also are represented. Expression patterns of several of these genes were studied in various tissues and organs of pine. Sequencing novel genes expressed during xylem formation will provide a powerful means of identifying mechanisms controlling this important differentiation pathway.

Non-NASA Center↗

Simple, Robust Cryogenic Propellant Depot for Near Term Applications

The ability to refuel cryogenic propulsion stages on-orbit provides an innovative paradigm shift for space transportation supporting National Aeronautics and Space Administration s (NASA) Exploration program as well as deep space robotic, national security and commercial missions. Refueling enables large beyond low Earth orbit (LEO) missions without requiring super heavy lift vehicles that must continuously grow to support increasing mission demands as America s exploration transitions from early Lagrange point missions to near Earth objects (NEO), the lunar surface and eventually Mars. Earth-to-orbit launch can be optimized to provide competitive, cost-effective solutions that allow sustained exploration. This paper describes an experimental platform developed to demonstrate the major technologies required for fuel depot technology. This test bed is capable of transferring residual liquid hydrogen (LH2) or liquid oxygen (LO2) from a Centaur upper stage, and storage in a secondary tank for up to one year on-orbit. A dedicated, flight heritage spacecraft bus is attached to an Evolved Expendable Launch Vehicle (EELV) Secondary Payload Adapter (ESPA) ring supporting experiments and data collection. This platform can be deployed as early as Q1 2013. The propellant depot design described in this paper can be deployed affordably this decade supporting missions to Earth-Moon Lagrange points and lunar fly by. The same depot concept can be scaled up to support more demanding missions and launch capabilities. The enabling depot design features, technologies and concept of operations are described.

McLean, Christopher↗