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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 325 records · Page 18
All sourcesNASA NTRSDOE OSTI
DOE OSTI2020

Optimizing the Design of Diatom Biosilica-Targeted Fusion Proteins in Biosensor Construction for Bacillus anthracis Detection

In vivo functionalization of diatom biosilica frustules by genetic manipulation requires careful consideration of the overall structure and function of complex fusion proteins. Although we previously had transformed Thalassiosira pseudonana with constructs containing a single domain antibody (sdAb) raised against the Bacillus anthracis Sterne strain, which detected an epitope of the surface layer protein EA1 accessible in lysed spores, we initially were unsuccessful with constructs encoding a similar sdAb that detected an epitope of EA1 accessible in intact spores and vegetative cells. This discrepancy limited the usefulness of the system as an environmental biosensor for B. anthracis. We surmised that to create functional biosilica-localized biosensors with certain constructs, the biosilica targeting and protein trafficking functions of the biosilica-targeting peptide Sil3T8 had to be uncoupled. We found that retaining the ER trafficking sequence at the N-terminus and relocating the Sil3T8 targeting peptide to the C-terminus of the fusion protein resulted in successful detection of EA1 with both sdAbs. Homology modeling of antigen binding by the two sdAbs supported the hypothesis that the rescue of antigen binding in the previously dysfunctional sdAb was due to removal of steric hindrances between the antigen binding loops and the diatom biosilica for that particular sdAb.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2011

Non-contiguous finished genome sequence and contextual data of the filamentous soil bacterium Ktedonobacter racemifer type strain (SOSP1-21 T )

Ktedonobacter racemifer corrig. Cavaletti et al. 2007 is the type species of the genus Ktedonobacter, which in turn is the type genus of the family Ktedonobacteraceae, the type family of the order Ktedonobacterales within the class Ktedonobacteria in the phylum 'Chloroflexi'. Although K. racemifer shares some morphological features with the actinobacteria, it is of special interest because it was the first cultivated representative of a deep branching unclassified lineage of otherwise uncultivated environmental phylotypes tentatively located within the phylum 'Chloroflexi'. The aerobic, filamentous, non-motile, spore-forming Gram-positive heterotroph was isolated from soil in Italy. The 13,661,586 bp long non-contiguous finished genome consists of ten contigs and is the first reported genome sequence from a member of the class Ktedonobacteria. With its 11,453 protein-coding and 87 RNA genes, it is the largest prokaryotic genome reported so far. It comprises a large number of over-represented COGs, particularly genes associated with transposons, causing the genetic redundancy within the genome being considerably larger than expected by chance. This work is a part of the Genomic Encyclopedia of Bacteria and Archaea project.

59 BASIC BIOLOGICAL SCIENCES↗
NASA NTRS1971

Environmental microbiology as related to planetary quarantine

The experiments carried out to determine the effects of temperature and relative humidity on the survival rate of Bacillus subtillis var. niger spores are reported. The experiments were conducted in environmental chambers at temperatures of 75 and 90 C. Data are also included on the survival characteristics of the spores suspended in sucrose solutions at 90 C with water activities of 0.99, 0.9, and 0.85

Pflug, I. J.↗
NASA NTRS1972

Services provided in support of the planetary quarantine requirements of the National Aeronautics and Space Administration

The efficiency of a biodetection grinder, used to recover buried contamination, was tested using spacecraft components and laminated polystyrene strips containing Bacillus subtilis var. niger spores. The surfaces were decontaminated before tests. Results are given in tabular form. Tables are also given for heat resistance of bacteria spores, prevalence of bacteria in spacecraft before launch, and the types of bacteria found in Apollo 15 spacecraft components and command modules.

Favero, M. S.↗
NASA NTRS1972

Survival of bacterial isolates exposed to simulated Jovian trapped radiation belt electrons and solar wind protons

With missions to Jupiter, the spacecraft will be exposed for extended duration to solar wind radiation and the Jovian trapped radiation belt. This study is designed to determine the effect of these radiation environments on spacecraft bacterial isolates. The information can be used in the probability of contamination analysis for these missions. A bacterial subpopulation from Mariner Mars 1971 spacecraft (nine sporeforming and three nonsporeforming isolates) plus two comparative organisms, Staphylococcus epidermidis ATCC 17917 and a strain of Bacillus subtilis var. niger, were exposed to 2-, 12-, and 25-MeV electrons at different doses with simultaneous exposure to a vacuum of 0.0013 N/sqm at 20 and -20 C. The radioresistance of the subpopulation was dependent on the isolate, dose, and energy of electrons. Temperature affected the radioresistance of only the sporeforming isolates. Survival data indicated that spores were reduced approximately 1 log/1500 J/kg, while nonsporeforming isolates (micrococci) were reduced 1.5 to 2 logs/1500 J/kg with the exception of an apparent radioresistant isolate whose resistance approached that of the spores. The subpopulation was found to be less resistant to lower energy than to higher energy electrons.

Taylor, D. M.↗
NASA NTRS1972

Services provided in support of the planetary quarantine requirements

Tests were conducted to determine the dry heat resistance at 125 C of a naturally occurring bacterial spore population in a mixture of sieved vacuum cleaner dusts from Cape Kennedy. The dust was aerosolized in a special chamber and was allowed to settle on 32 Teflon ribbons to provide approximately 500,000 spores per ribbon.

Favero, M. S.↗
NASA NTRS1971

Microbiologic assay of space hardware.

Review of the procedures used in the microbiological examination of space hardware. The general procedure for enumerating aerobic and anaerobic microorganisms and spores is outlined. Culture media and temperature-time cycles used for incubation are reviewed, along with assay systems designed for the enumeration of aerobic and anaerobic spores. The special problems which are discussed are involved in the precise and accurate enumeration of microorganisms on surfaces and in the neutralization of viable organisms buried inside solid materials that could be released to a planet's surface if the solid should be fractured. Special attention is given to sampling procedures including also the indirect techniques of surface assays of space hardware such as those using detachable or fallout strips. Some data on comparative levels of microbial contamination on lunar and planetary spacecraft are presented.

Favero, M. S.↗
NASA NTRS1971

The Effect of Temperature on the Survival of Microorganisms in a Deep Space Vacuum

A space molecular sink research facility (Molsink) was used to evaluate the ability of microorganisms to survive the vacuum of outer space. This facility could be programmed to simulate flight spacecraft vacuum environments at pressures in the .1 nanotorr range and thermal gradients (30 to 60 C) closely associated to surface temperatures of inflight spacecraft. Initial populations of Staphylococcus epidermidis and a Micrococcus sp. were reduced approximately 1 log while exposed to -105 and 34 C, and approximately 2 logs while exposed to 59 C for 14 days in the vacuum environment. Spores of Bacillus subtilis var. niger were less affected by the environment. Initial spore populations were reduced 0.2, 0.3, and 0.8 log during the 14-day vacuum exposure at -124, 34, and 59 C, respectively.

Hagen, C. A.↗
NASA NTRS1972

Aliphatic hydrocarbons of the fungi.

Review of studies of aliphatic hydrocarbons which have been recently detected in the spores of phytopathogenic fungi, and are found to be structurally very similar to the alkanes of higher plants. It appears that the hydrocarbon components of the few mycelial and yeast forms reported resemble the distribution found in bacteria. The occurence and distribution of these compounds in the fungi is discussed. Suggested functional roles of fungal spore alkanes are presented.

Weete, J. D.↗
NASA NTRS1973

Ecology and thermal inactivation of microbes in and on interplanetary space vehicle components

An experimental sterilization facility was developed to simulate conditions that will be encountered during terminal sterilization of space vehicles. The system consists of a temperature controlled oven with a nitrogen gas stream containing a known concentration of water. Moisture analyzers are utilized to monitor the gas flowing over spore samples contained in the oven. In its original configuration, no provision was made for the control of water vapor during the sterilization cycle. Because moisture profoundly influences the thermal inactivation of bacterial spores, an upper limit for the moisture content in the gas used to sterilize the space vehicle was established (25% RH at 0 C STP). Accordingly, a controller was developed and installed to provide these conditions in the experimental sterilization facility.

Wimsatt, J. C.↗
NASA NTRS1972

Services provided in support of the planetary quarantine requirements of the National Aeronautics and Space Administration

Heat studies with the highly resistant bacterial spore isolated from Cape Kennedy soil were continued, and the D130C was determined. The interior surfaces of the command module of the Apollo 17 spacecraft were studied for microbial contamination during assembly and testing. The thermal resistance of naturally occurring airborne bacterial spores was determined, using the heating times of 2, 4, 6, and 8 hr. at 125 C. The evaluation of a terminal sterilization process for unmanned lander spacecraft is also continuing.

Favero, M. S.↗
NASA NTRS1973

Postflight analyses of Bacillus thuringiensis organisms exposed to space flight conditions

Cultures of B. thuringiensis returned from space flight appeared to be normal to slightly affected adversely in their ability to produce three toxins that affect insects. In addition, it can be stated that B. thuringiensis spores are very resistant to ultraviolet irradiation at the individual wavelengths and energy levels previously described. Full sunlight, however, does have a detrimental effect on the viability of B. thuringiensis spores.

Wrenn, R. T.↗
NASA NTRS1973

Thermoradiation inactivation of naturally occurring organisms in soil

Samples of soil collected from Kennedy Space Center near spacecraft assembly facilities were found to contain microorganisms very resistant to conventional sterilization techniques. The inactivation behavior of the naturally occurring spores in soil was investigated using dry heat and ionizing radiation, first separately, then in combination. Dry heat inactivation rates of spores were determined for 105 and 125 C. Radiation inactivation rates were determined for dose rates of 660 and 76 krad/hr at 25 C. Simultaneous combinations of heat and radiation were then investigated at 105, 110, 115, 120, and 125 C. Combined treatment was found to be highly synergistic requiring greatly reduced radiation doses to accomplish sterilization.

Reynolds, M. C.↗
NASA NTRS1973

Ecology and thermal inactivation of microbes in and on interplanetary space vehicle components

The thermal inactivation curve for Bacillus subtilis var. niger spores on the Viking lander is examined. Tests were conducted at 113 C and 25% RH, and over a wide range of temperatures using .001% RH and additions of P2O5 to dry the environment. Results show the 25% RH environment did not significantly reduce the survival curve, while the survival curves for spores treated under the drier .001% RH environment was reduced by a factor of 3.

Campbell, J. E.↗
NASA NTRS1974

A study of psychrophilic organisms isolated from the manufacture and assembly areas of spacecraft to be used in the Viking mission

The effect of storage of dry heat treated Teflon ribbons under nitrogen gas followed by high vacuum on the recovery of hardy organisms from the ribbons was studied. A similar experiment was performed on spore crops of hardy organisms recovered previously from Cape Canaveral. Hardy organisms have been inoculated onto slides and subjected to an artificial Martian environment in an attempt to demonstrate their growth in this environment. Additional experiments using the artificial Martian environment include response of soil samples from the VAB with both constant temperature and freeze-thaw cycles. These experiments were performed with dried soil and soil containing added water. Other investigations included the effect of heatshock on soil samples, psychrophilic counts of new soil samples from the manufacture area of the Viking spacecraft, effect of pour plate versus spread plate on psychrophilic counts, and preparation of spore crops of hardy organisms from Cape Canaveral.

Foster, T. L.↗
NASA NTRS1974

Ecology and thermal inactivation of microbes in and on interplanetary space vehicle components

The uses of scanning electron microscopy in assessing changes that occur in spores exposed to wet and dry heat cycles at elevated temperatures were examined. Several species of Bacillus and other nonspore-forming species of organisms were used for the experiment. Surface morphology of viable and nonviable organisms was clearly detectable by this method, making it a potentially useful technique for investigating microbial inactivation on space vehicle surfaces and components. Micrographs of the spores and bacterial cells are provided.

Campbell, J. E.↗