Proceedings of the Microbial Response to Space Environment Symposium
Conference on Apollo 16 experiment for measuring microbial response to space environment
Taylor, G. R.↗
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Description of the microbial ecology evaluation device, flight equipment, and ground transporter
Exposure of test systems in space required the fabrication of specialized hardware termed a Microbial Ecology Evaluation Device that had individual test chambers and a complex optical filter system. The characteristics of this device and the manner in which it was deployed in space are described.
Chassay, C. E.↗
The ferrioxalate actinometry system of the microbial response to space environment experiment (M191)
The fluid actinometry portion of the Microbial Response to Space Environment Experiment (M191) was designed for measurement of the solar energy that penetrates certain optical filter systems during exposure in space. Potassium ferrioxalate was used to measure energy at peak wavelengths of 254, 280, and 300 nanometers because of its high degree of sensitivity and its linear response to the middle ultraviolet regions.
Parson, M.↗
Thermoluminescent dosimetry for the Apollo 16 microbial response to space environment experiment (M191)
Lithium fluoride thermoluminescent chips were used to provide an integrated dose from the broad spectrum of ionizing radiation to the Microbial Response to Space Environment Experiment (M191). The chips were positioned in the flight hardware to provide data on ionizing radiation within specific volume segments. A uniform radiation dose of 4.8 x 0.001 plus or minus 2 x 0.0001 joule/kg resulted.
Brown, R. D.↗
Spacecraft microbial burden reduction due to atmospheric entry heating: Jupiter
Planetary quarantine analyses performed for recent unmanned Mars and Venus missions assumed that the probability of contamination by a spacecraft given accidental impact was equivalent to one. However, in the case of the gaseous outer planets, the heat generated during the inadvertent entry of a spacecraft into the planetary atmosphere might be sufficient to cause significant microbial burden reduction. This could affect navigation strategy by reducing the necessity for biasing the aim point away from the planets. An effort has been underway to develop the tools necessary to predict temperature histories for a typical spacecraft during inadvertent entry. In order that the results have general applicability, parametric analyses were performed. The thermal response of the spacecraft components and debris resulting from disintegration was determined. The temperature histories of small particles and composite materials, such as thermal blankets and an antenna, were given special attention. Guidelines are given to indicate the types of components and debris most likely to contain viable organisms, which could contaminate the lower layers of the Jovian atmosphere (approximately one atmosphere of pressure).
Gonzalez, C. C.↗
Studies on possible propagation of microbial contamination in planetary clouds
One of the key parameters in estimation of the probability of contamintion of the outer planets (Jupiter, Saturn, Uranus, etc.) is the probability of growth (Pg) of terrestrial microorganisms on or near these planets. For example, Jupiter appears to have an atmosphere in which some microbial species could metabolize and propagate. This study includes investigation of the likelihood of metabolism and propagation of microbes suspended in dynamic atmospheres. It is directed toward providing experimental information needed to aid in rational estimation of Pg for these outer planets. Current work is directed at demonstration of aerial metabolism under near optimal conditions and tests of propagation in simulated Jovian atmospheres.
Dimmick, R. L.↗
Automated Microbial Metabolism Laboratory
Development of the automated microbial metabolism laboratory (AMML) concept is reported. The focus of effort of AMML was on the advanced labeled release experiment. Labeled substrates, inhibitors, and temperatures were investigated to establish a comparative biochemical profile. Profiles at three time intervals on soil and pure cultures of bacteria isolated from soil were prepared to establish a complete library. The development of a strategy for the return of a soil sample from Mars is also reported.
Source record↗
A rapid method for the determination of microbial susceptibility using the firefly luciferase assay for adenosine triphosphate (ATP)
Luciferase assay for adenosine triphosphate (ATP) was optimized for pure bacteria in broth in order to evaluate if changes in bacterial ATP content could be used as a rapid measure of antibiotic effect on microorganisms. Broth cultures of log phase bacteria were incubated at 310 K (37 C) for 2.5 hours at antimicrobial concentrations which resulted in the best discrimination between sensitive and resistant strains. Eighty-seven strains of 11 bacterial species were studied for their susceptibility to 12 commonly used antimicrobial agents: ampicillin, Penicillin G, nafcillin, carbenicillin, cephalothin, tetracycline, erythromycin, clindamycin, gentamicin, nitrofurantoin, colistin, and chloramplenicol. The major advantage of the ATP system over existing methods of rapid microbial susceptibility testing is that the assay can be made specific for bacterial ATP.
Vellend, H.↗
Microbial exchange experiment AR-002
Operational aspects associated with the experiment and the activities of medically important microorganisms recovered from the Apollo crewmen are evaluated. A variety of potential pathogens was recovered from each of the prime and backup crew members before and after flight. However, no disease events were reported. Candida albicans and Staphylococcus aureus were shown to be transferred from one crewmember to another during the flight. No other medically significant changes in the microbial population were observed.
Taylor, G. R.↗
Microbial load monitor
Design analysis of a microbial load monitor system flight engineering model was presented. Checkout of the card taper and media pump system was fabricated as well as the final two incubating reading heads, the sample receiving and card loading device assembly, related sterility testing, and software. Progress in these areas was summarized.
Caplin, R. S.↗
Microbial load monitor
Attempts are made to provide a total design of a Microbial Load Monitor (MLM) system flight engineering model. Activities include assembly and testing of Sample Receiving and Card Loading Devices (SRCLDs), operator related software, and testing of biological samples in the MLM. Progress was made in assembling SRCLDs with minimal leaks and which operate reliably in the Sample Loading System. Seven operator commands are used to control various aspects of the MLM such as calibrating and reading the incubating reading head, setting the clock and reading time, and status of Card. Testing of the instrument, both in hardware and biologically, was performed. Hardware testing concentrated on SRCLDs. Biological testing covered 66 clinical and seeded samples. Tentative thresholds were set and media performance listed.
Caplin, R. S.↗
New microbial growth factor
A screening procedure was used to isolate from soil a Penicillium sp., two bacterial isolates, and a Streptomyces sp. that produced a previously unknown microbial growth factor. This factor was an absolute growth requirement for three soil bacteria. The Penicillium sp. and one of the bacteria requiring the factor, an Arthrobacter sp., were selected for more extensive study concerning the production and characteristics of the growth factor. It did not seem to be related to the siderochromes. It was not present in soil extract, rumen fluid, or any other medium component tested. It appears to be a glycoprotein of high molecular weight and has high specific activity. When added to the diets for a meadow-vole mammalian test system, it caused an increased consumption of diet without a concurrent increase in rate of weight gain.
Bok, S. H.↗
Indirect microbial detection
Indirect method for detection of microbial growth utilizes flow of charged particles across barrier that physically separated growing cells from electrodes and measures resulting difference in potential between two platinum electrodes. Technique allows simplified noncontact monitoring of all growth in highly infectious cultures or in critical biochemical studies.
Wilkins, J. R.↗
Water system microbial check valve development
A residual iodine microbial check valve (RIMCV) assembly was developed and tested. The assembly is designed to be used in the space shuttle potable water system. The RIMCV is based on an anion exchange resin that is supersaturated with an iodine solution. This system causes a residual to be present in the effluent water which provides continuing bactericidal action. A flight prototype design was finalized and five units were manufactured and delivered.
Colombo, G. V.↗
Microbial Check Valve for Shuttle
The Microbial Check Valve (MCV) is a device developed for the Space Shuttle that prevents the transfer of viable microorganisms within water systems. The device is essentially a bed of resin material, impregnated with iodine, that kills microorganisms on contact. It prevents the cross-contamination of microorganisms from a nonpotable system into the potable water system when these systems are interconnected. In this regard, the function of the device is similar to that of the 'air gap' found in conventional one-gravity systems. Basic design data are presented including pressure drop, scaling factors, sizing criteria, and the results of challenging the device with suspensions of seven microorganisms including aerobes, anaerobes and spore formers.
Colombo, G. V.↗
Advanced microbial check valve development
A flight certified assembly identified as a Microbial Check Valve (MCV) was developed and tested. The MCV is a canister packed with an iodinated anionic exchange resin. The device is used to destroy organisms in a water stream as the water passes through the device. The device is equally effective for fluid flow in either direction and its primary method of organism removal is killing rather than filtering. The MCV was successfully developed for the space shuttle to: disinfect fuel cell water; and prevent back contamination of the stored potable water supply. One version of the device consists of a high residual iodinated resin bed that imparts approximately 2 ppm of iodine to the fuel cell water as it flows to the potable water tanks. A second version of the device consists of a low residual iodinated resin bed. One of these low residual beds is located at each use port in the potable water system for the dual purpose of removing some iodine from the potable water as it is dispensed and also to prevent back contamination of the potable supply.
Colombo, G. V.↗
Endolithic microbial life in hot and cold deserts
Endolithic microorganisms (those living inside rocks) occur in hot and cold deserts and exist under extreme environmental conditions. These conditions are discussed on a comparative basis. Quantitative estimates of biomass are comparable in hot and cold deserts. Despite the obvious differences between the hot and cold desert environment, survival strategies show some common features. These endolithic organisms are able to 'switch' rapidly their metabolic activities on and off in response to changes in the environment. Conditions in hot deserts impose a more severe environmental stress on the organisms than in the cold Antarctic desert. This is reflected in the composition of the microbial flora which in hot desert rocks consist entirely of prokaryotic microorganisms, while under cold desert conditions eukaryotes predominate.
Friedmann, E. I.↗
Indirect microbial detection
The growth of microorganisms in a sample is detected and monitored by culturing microorganisms in a growth medium and detecting a change in potential between two electrodes, separated from the microbial growth by a barrier which is permeable to charged paticles but microorganism impermeable.
Wilkins, J. R.↗