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At least 325 records · Page 18

Cytoplasmic pH dynamics in maize pulvinal cells induced by gravity vector changes

In maize (Zea mays) and other grasses, changes in orientation of stems are perceived by pulvinal tissue, which responds to the stimulus by differential growth resulting in upward bending of the stem. The amyloplast-containing bundle sheath cells are the sites of gravity perception, although the initial steps of gravity perception and transmission remain unclear. In columella cells of Arabidopsis roots, we previously found that cytoplasmic pH (pH(c)) is a mediator in early gravitropic signaling (A.C. Scott, N.S. Allen [1999] Plant Physiol 121: 1291-1298). The question arises whether pH(c) has a more general role in signaling gravity vector changes. Using confocal ratiometric imaging and the fluorescent pH indicator carboxy seminaphtorhodafluor acetoxymethyl ester acetate, we measured pH(c) in the cells composing the maize pulvinus. When stem slices were gravistimulated and imaged on a horizontally mounted confocal microscope, pH(c) changes were only apparent within the bundle sheath cells, and not in the parenchyma cells. After turning, cytoplasmic acidification was observed at the sides of the cells, whereas the cytoplasm at the base of the cells where plastids slowly accumulated became more basic. These changes were most apparent in cells exhibiting net amyloplast sedimentation. Parenchyma cells and isolated bundle sheath cells did not show any gravity-induced pH(c) changes although all cell types responded to external stimuli in the predicted way: Propionic acid and auxin treatments induced acidification, whereas raising the external pH caused alkalinization. The results suggest that pH(c) has an important role in the early signaling pathways of maize stem gravitropism.

Non-NASA Center↗

Spaceflight reduces somatic embryogenesis in orchardgrass (Poaceae)

Somatic embryos initiate and develop from single mesophyll cells in in vitro cultured leaf segments of orchard-grass (Dactylis glomerata L.). Segments were plated at time periods ranging from 21 to 0.9 d (21 h) prior to launch on an 11 d spaceflight (STS-64). Using a paired t-test, there was no significant difference in embryogenesis from preplating periods of 14 d and 21 d. However, embryogenesis was reduced by 70% in segments plated 21 h before launch and this treatment was significant at P=0.0001. The initial cell divisions leading to embryo formation would be taking place during flight in this treatment. A higher ratio of anticlinal:periclinal first cell divisions observed in the flight compared to the control tissue suggests that microgravity affects axis determination and embryo polarity at a very early stage. A similar reduction in zygotic embryogenesis would reduce seed formation and have important implications for long-term space flight or colonization where seeds would be needed either for direct consumption or to grow another generation of plants.

NASA Discipline Plant Biology↗

MRI of bone marrow in the distal radius: in vivo precision of effective transverse relaxation times

The effective transverse relaxation time T2* is influenced by the presence of trabecular bone, and can potentially provide a measure of bone density as well as bone structure. We determined the in vivo precision of T2* in repeated bone marrow measurements. The T2* measurements of the bone marrow of the distal radius were performed twice within 2 weeks in six healthy young volunteers using a modified water-presaturated 3D Gradient-Recalled Acquisition at Steady State (GRASS) sequence with TE 7, 10, 12, 20, and 30; TR 67; flip angle (FA) 90 degrees. An axial volume covering a length of 5.6 cm in the distal radius was measured. Regions of interest (ROIs) were determined manually and consisted of the entire trabecular bone cross-section extending proximally from the radial subchondral endplate. Reproducibility of T2* and area measurements was expressed as the absolute precision error (standard deviation [SD] in ms or mm2) or as the relative precision error (SD/mean x 100, or coefficient of variation [CV] in %) between the two-point measurements. Short-term precision of T2* and area measurements varied depending on section thickness and location of the ROI in the distal radius. Absolute precision errors for T2* times were between 1.3 and 2.9 ms (relative precision errors 3.8-9.5 %) and for area measurements between 20 and 55 mm2 (relative precision errors 5.1-16.4%). This MR technique for quantitative assessment of trabecular bone density showed reasonable reproducibility in vivo and is a promising future tool for the assessment of osteoporosis.

NASA Discipline Musculoskeletal↗

Two endogenous proteins that induce cell wall extension in plants

Plant cell enlargement is regulated by wall relaxation and yielding, which is thought to be catalyzed by elusive "wall-loosening" enzymes. By employing a reconstitution approach, we found that a crude protein extract from the cell walls of growing cucumber seedlings possessed the ability to induce the extension of isolated cell walls. This activity was restricted to the growing region of the stem and could induce the extension of isolated cell walls from various dicot stems and the leaves of amaryllidaceous monocots, but was less effective on grass coleoptile walls. Endogenous and reconstituted wall extension activities showed similar sensitivities to pH, metal ions, thiol reducing agents, proteases, and boiling in methanol or water. Sequential HPLC fractionation of the active wall extract revealed two proteins with molecular masses of 29 and 30 kD associated with the activity. Each protein, by itself, could induce wall extension without detectable hydrolytic breakdown of the wall. These proteins appear to mediate "acid growth" responses of isolated walls and may catalyze plant cell wall extension by a novel biochemical mechanism.

Non-NASA Center↗

The Widest Practicable Dissemination: The NASA Technical Report Server

The National Aeronautics and Space Act of 1958 established NASA and charged it to "provide for the widest practicable and appropriate dissemination of information concerning [...] its activities and the results thereof." The search for innovative methods to distribute NASA s information lead a grass-roots team to create the NASA Technical Report Server (NTRS), which uses the World Wide Web and other popular Internet-based information systems as search engines. The NTRS is an inter-center effort which provides uniform access to various distributed publication servers residing on the Internet. Users have immediate desktop access to technical publications from NASA centers and institutes. The NTRS is comprised of several units, some constructed especially for inclusion in NTRS, and others that are existing NASA publication services that NTRS reuses. This paper presents the NTRS architecture, usage metrics, and the lessons learned while implementing and maintaining the services over the initial 6-month period. The NTRS is largely constructed with freely available software running on existing hardware. NTRS builds upon existing hardware and software, and the resulting additional exposure for the body of literature contained will allow NASA to ensure that its institutional knowledge base will continue to receive the widest practicable and appropriate dissemination.

Nelson, Michael L.↗

Analysis and expression of the alpha-expansin and beta-expansin gene families in maize

Expansins comprise a multigene family of proteins in maize (Zea mays). We isolated and characterized 13 different maize expansin cDNAs, five of which are alpha-expansins and eight of which are beta-expansins. This paper presents an analysis of these 13 expansins, as well as an expression analysis by northern blotting with materials from young and mature maize plants. Some expansins were expressed in restricted regions, such as the beta-expansins ExpB1 (specifically expressed in maize pollen) and ExpB4 (expressed principally in young husks). Other expansins such as alpha-expansin Exp1 and beta-expansin ExpB2 were expressed in several organs. The expression of yet a third group was not detected in the selected organs and tissues. An analysis of expansin sequences from the maize expressed sequence tag collection is also presented. Our results indicate that expansin genes may have general, overlapping expression in some instances, whereas in other cases the expression may be highly specific and limited to a single organ or cell type. In contrast to the situation in Arabidopsis, beta-expansins in maize seem to be more numerous and more highly expressed than are alpha-expansins. The results support the concept that beta-expansins multiplied and evolved special functions in the grasses.

NASA Program Fundamental Space Biology↗

The NASA Technical Report Server

The National Aeronautics and Space Act of 1958 established NASA and charged it to "provide for the widest practicable and appropriate dissemination of information concerning its activities and the results thereof." The search for innovative methods to distribute NASA's information lead a grass-roots team to create the NASA Technical Report Server (NTRS), which uses the World Wide Web and other popular Internet-based information systems as search engines. The NTRS is an inter-center effort which provides uniform access to various distributed publication servers residing on the Internet. Users have immediate desktop access to technical publications from NASA centers and institutes. The NTRS is comprised of several units, some constructed especially for inclusion in NTRS, and others that are existing NASA publication services that NTRS reuses. This paper presents the NTRS architecture, usage metrics, and the lessons learned while implementing and maintaining the service. The NTRS is largely constructed with freely available software running on existing hardware. NTRS builds upon existing hardware and software, and the resulting additional exposure for the body of literature contained ensures that NASA's institutional knowledge base will continue to receive the widest practicable and appropriate dissemination.

Nelson, Michael L.↗

The Global Impact of Biomass Burning: An Interview with EPA's Robert Huggett

The extent of biomass burning has increased significantly over the past 100 years because of human activities, and such burning is much more frequent and widespread than was previously believed. Biomass burning is now recognized as a significant global source of emissions, contributing as much as 40% of gross carbon dioxide and 38% of tropospheric ozone. Most of the world's burned biomass matter is from the savannas, and because two-thirds of the Earth's savannas are located in Africa, that continent is now recognized as the "burn center" of the planet. In the past few years the international scientific community has conducted field experiments using ground-based and airborne measurements in Africa, South America. and Siberia to better assess the global production of gases and particulates by biomass burning. Researchers are gathering this month in Williamsburg, VA, to discuss the results of these and other investigations at the Second Chapman Conference on Biomass Burning and Global Change, sponsored by the American Geophysical Union. The first international biomass burning conference, held in 1990, was attended by atmospheric chemists, climatologists, ecologists, forest and soil scientists, fire researchers, remote- sensins specialists, and environmental planners and managers from more than 25 countries.When we hear about biomass burning, we usually think of the burning of the worlds tropical forests for permanent land clearing. However, biomass burning serves a variety of land use changes, including the clearing of forests and savannas for agricultural and grazing use; shifting agriculture practices; the control of grass, weeds, and litter on agricultural and grazing lands; the elimination of stubble and waste on agricultural lands after the harvest; and the domestic use of biomass matter.

Sevine, Joel S.↗

Seasonal Distribution of African Savanna Fires

Savannas consist of a continuous layer of grass interspersed with scattered trees or shrubs, and cover approx. 10 million square kilometers of tropical Africa. African savanna fires, almost all resulting from human activities, may produce as much as a third of the total global emissions from biomass burning. Little is known, however, about the frequency and location of these fires, and the area burned each year. Emissions from African savanna burning are known to be transported over the mid-Atlantic, south Pacific and Indian oceans; but to study fully the transport of regional savanna burning and the seasonality of the atmospheric circulation must be considered simultaneously. Here we describe the temporal and spatial distribution of savanna fires over the entire African continent, as determined from night-time satellite imagery. We find that, contrary to expectations, most fires are left to burn uncontrolled, so that there is no strong diurnal cycle in the fire frequency. The knowledge gained from this study regarding the distribution and variability of fires will aid monitoring of the climatically important trace gases emitted from burning biomass.

Cahoon, Donald R.↗

New genes and new biological roles for expansins

Expansins are extracellular proteins that loosen plant cell walls in novel ways. They are thought to function in cell enlargement, pollen tube invasion of the stigma (in grasses), wall disassembly during fruit ripening, abscission and other cell separation events. Expansins are encoded by two multigene families and each gene is often expressed in highly specific locations and cell types. Structural analysis indicates that one expansin region resembles the catalytic domain of family-45 endoglucanases but glucanase activity has not been detected. The genome projects have revealed numerous expansin-related sequences but their putative wall-loosening functions remain to be assessed.

NASA Program Fundamental Space Biology↗

Comparison of Landsat-7 Enhanced Thematic Mapper Plus (ETM+) and Earth Observing One (EO-1) Advanced Land Imager

We compare images from the Enhanced Thematic Mapper Plus (ETM+) sensor on Landsat-7 and the Advanced Land Imager (ALI) instrument on Earth Observing One (EO-1) over a test site in Rochester, New York. The site contains a variety of features, ranging from water of varying depths, deciduous/coniferous forest, and grass fields, to urban areas. Nearly coincident cloud-free images were collected one minute apart on 25 August 2001. We also compare images of a forest site near Howland, Maine, that were collected on 7 September, 2001. We atmospherically corrected each pair of images with the Second Simulation of the Satellite Signal in the Solar Spectrum (6S) atmosphere model, using aerosol optical thickness and water vapor column density measured by in situ Cimel sun photometers within the Aerosol Robotic Network (AERONET), along with ozone density derived from the Total Ozone Mapping Spectrometer (TOMS) on the Earth Probe satellite. We present true-color composites from each instrument that show excellent qualitative agreement between the multispectral sensors, along with grey-scale images that demonstrate a significantly improved ALI panchromatic band. We quantitatively compare ALI and ETM+ reflectance spectra of a grassy field in Rochester and find < or equal to 6% differences in the visible/near infrared and approx. 2% differences in the short wave infrared. Spectral comparisons of forest sites in Rochester and Howland yield similar percentage agreement except for band 1, which has very low reflectance. Principal component analyses and comparison of normalized difference vegetation index histograms for each sensor indicate that the ALI is able to reproduce the information content in the ETM+ but with superior signal-to-noise performance due to its increased 12-bit quantization.

Pedelty, Jeffrey A.↗

Predictions of adult Anopheles albimanus densities in villages based on distances to remotely sensed larval habitats

Remote sensing is particularly helpful for assessing the location and extent of vegetation formations, such as herbaceous wetlands, that are difficult to examine on the ground. Marshes that are sparsely populated with emergent macrophytes and dense cyanobacterial mats have previously been identified as very productive Anopheles albimanus larval habitats. This type of habitat was detectable on a classified multispectral System Probatoire d'Observation de la Terre image of northern Belize as a mixture of two isoclasses. A similar spectral signature is characteristic for vegetation of river margins consisting of aquatic grasses and water hyacinth, which constitutes another productive larval habitat. Based on the distance between human settlements (sites) of various sizes and the nearest marsh/river exhibiting this particular class combination, we selected two groups of sites: those located closer than 500 m and those located more than 1,500 m from such habitats. Based on previous adult collections near larval habitats, we defined a landing rate of 0.5 mosquitoes/human/min from 6:30 PM to 8:00 PM as the threshold for high (> or = 0.5 mosquitoes/human/min) versus low (< 0.5 mosquitoes/human/min) densities of An. albimanus. Sites located less than 500 m from the habitat were predicted as having values higher than this threshold, while lower values were predicted for sites located greater than 1,500 m from the habitat. Predictions were verified by collections of mosquitoes landing on humans. The predictions were 100% accurate for sites in the > 1,500-m category and 89% accurate for sites in the < 500-m category.

NASA Discipline General Space Life Sciences↗

Applications of Principled Search Methods in Climate Influences and Mechanisms

Forest and grass fires cause economic losses in the billions of dollars in the U.S. alone. In addition, boreal forests constitute a large carbon store; it has been estimated that, were no burning to occur, an additional 7 gigatons of carbon would be sequestered in boreal soils each century. Effective wildfire suppression requires anticipation of locales and times for which wildfire is most probable, preferably with a two to four week forecast, so that limited resources can be efficiently deployed. The United States Forest Service (USFS), and other experts and agencies have developed several measures of fire risk combining physical principles and expert judgment, and have used them in automated procedures for forecasting fire risk. Forecasting accuracies for some fire risk indices in combination with climate and other variables have been estimated for specific locations, with the value of fire risk index variables assessed by their statistical significance in regressions. In other cases, the MAPSS forecasts [23, 241 for example, forecasting accuracy has been estimated only by simulated data. We describe alternative forecasting methods that predict fire probability by locale and time using statistical or machine learning procedures trained on historical data, and we give comparative assessments of their forecasting accuracy for one fire season year, April- October, 2003, for all U.S. Forest Service lands. Aside from providing an accuracy baseline for other forecasting methods, the results illustrate the interdependence between the statistical significance of prediction variables and the forecasting method used.

Glymour, Clark↗

Foliage discrimination using a rotating ladar

We present a real time algorithm that detects foliage using range from a rotating laser. Objects not classified as foliage are conservatively labeled as non-driving obstacles. In contrast to related work that uses range statistics to classify objects, we exploit the expected localities and continuities of an obstacle, in both space and time. Also, instead of attempting to find a single accurate discriminating factor for every ladar return, we hypothesize the class of some few returns and then spread the confidence (and classification) to other returns using the locality constraints. The Urbie robot is presently using this algorithm to descriminate drivable grass from obstacles during outdoor autonomous navigation tasks.

Autonomous navigation range robots↗

Alternate-Fueled Flight: Halophytes, Algae, Bio-, and Synthetic Fuels

Synthetic and biomass fueling are now considered to be near-term aviation alternate fueling. The major impediment is a secure sustainable supply of these fuels at reasonable cost. However, biomass fueling raises major concerns related to uses of common food crops and grasses (some also called "weeds") for processing into aviation fuels. These issues are addressed, and then halophytes and algae are shown to be better suited as sources of aerospace fuels and transportation fueling in general. Some of the history related to alternate fuels use is provided as a guideline for current and planned alternate fuels testing (ground and flight) with emphasis on biofuel blends. It is also noted that lessons learned from terrestrial fueling are applicable to space missions. These materials represent an update and additions to the Workshop on Alternate Fueling Sustainable Supply and Halophyte Summit at Twinsburg, OH, Oct. 17 to 18, 2007 (ref. 1).

Hendricks, R. C.↗

On Convergence of Development Costs and Cost Models for Complex Spaceflight Instrument Electronics

Development costs of a few recent spaceflight instrument electrical and electronics subsystems have diverged from respective heritage cost model predictions. The cost models used are Grass Roots, Price-H and Parametric Model. These cost models originated in the military and industry around 1970 and were successfully adopted and patched by NASA on a mission-by-mission basis for years. However, the complexity of new instruments recently changed rapidly by orders of magnitude. This is most obvious in the complexity of representative spaceflight instrument electronics' data system. It is now required to perform intermediate processing of digitized data apart from conventional processing of science phenomenon signals from multiple detectors. This involves on-board instrument formatting of computational operands from row data for example, images), multi-million operations per second on large volumes of data in reconfigurable hardware (in addition to processing on a general purpose imbedded or standalone instrument flight computer), as well as making decisions for on-board system adaptation and resource reconfiguration. The instrument data system is now tasked to perform more functions, such as forming packets and instrument-level data compression of more than one data stream, which are traditionally performed by the spacecraft command and data handling system. It is furthermore required that the electronics box for new complex instruments is developed for one-digit watt power consumption, small size and that it is light-weight, and delivers super-computing capabilities. The conflict between the actual development cost of newer complex instruments and its electronics components' heritage cost model predictions seems to be irreconcilable. This conflict and an approach to its resolution are addressed in this paper by determining the complexity parameters, complexity index, and their use in enhanced cost model.

Kizhner, Semion↗

The Joint Aerosol-Monsoon Experiment (JAMEX): A Core Element for the Asian Monsoon Year (2008-2009)

The objective of the Joint Aerosol-Monsoon Experiment (JAMEX) is to unravel the physical mechanisms and multi-scale interactions associated with aerosol-monsoon water cycle in the Asian Indo-Pacific region towards improved prediction of rainfall in land regions of the Asian monsoon. JAMEX will be planned as a five-year (2007-201 1) multi-national aerosol-monsoon research project, aimed at promoting collaboration, partnership and alignment of ongoing and planned national and international programs. Two coordinated special observing periods (SOP), covering the pre-monsoon (April-May) and the monsoon (June-August) periods is tentatively targeted for 2008 and 2009. The major work on validation and reference site coordination will take place in 2007 through the spring of 2008. A major science workshop is planned after SOP-I1 in 2010. Modeling and satellite data utilization studies will continue throughout the entire period to help in design of the observation arrays and measurement platforms for SOPS. The tentative time schedule, including milestones and research activities is shown in Fig. 1. One of the unique aspects of JAMEX is that it stems from grass-root scientific and societal imperatives, and it bridges a gap in existing national and international research programs. Currently we have identified 10 major national and international projects/programs separately for aerosols and monsoon research planned in the next five years in China, India, Japan, Italy, and the US, that could be potential contributors or partners with JAMEX. These include the Asian-Indo- Pacific Ocean (AIPO) Project and Aerosol Research Project from China, Monsoon Asian Hydro- Atmospheric Science Research and predication Initiative (MAHASRI) from Japan, Continental Tropical Convergence Zone (CTCZ) and Severe Thunderstorm: Observations and Regional Modeling (STORM) from India, Share-Asia from Italy, Atmospheric Brown Cloud (ABC), Pacific Aerosol-Cloud-Dust Experiment (PACDEX), East Asia Study of Tropospheric Aerosol: an International Regional Experiment (East-AIRE), and Radiation Aerosol Joint Observations - Monsoon Experiments over the Gangetic Himalayas Area (Rajo-Megha: dust cloud in Sanskrit) from the US, and Monsoon Asia Integrated Regional Study (MAIR) under the Earth Systems I Science Partnership (ESSP) and WCRP. For JAMEX to succeed, it is crucial for an international body, such as CEOP or an organization under WCRP to provide the science oversight, data policy and stewardship, and to promote collaboration and partnership among national programs. It makes eminent sense for WCRP to expand the concept and the prototype proposed by JAMEX to include all monsoon countries to expand AMY08-09 into an International Monsoon Era (2008- 2013). Such an establishment followed by establishment of an international body for science oversight, and data stewardship will go a long way in promoting coordination and connection among various existing monsoon research programs within WCRP, and with burgeoning national programs on monsoon and aerosol research.

Lau, William K.M.↗

Microwave Soil Moisture Retrieval Under Trees

Soil moisture is recognized as an important component of the water, energy, and carbon cycles at the interface between the Earth's surface and atmosphere. Current baseline soil moisture retrieval algorithms for microwave space missions have been developed and validated only over grasslands, agricultural crops, and generally light to moderate vegetation. Tree areas have commonly been excluded from operational soil moisture retrieval plans due to the large expected impact of trees on masking the microwave response to the underlying soil moisture. Our understanding of the microwave properties of trees of various sizes and their effect on soil moisture retrieval algorithms at L band is presently limited, although research efforts are ongoing in Europe, the United States, and elsewhere to remedy this situation. As part of this research, a coordinated sequence of field measurements involving the ComRAD (for Combined Radar/Radiometer) active/passive microwave truck instrument system has been undertaken. Jointly developed and operated by NASA Goddard Space Flight Center and George Washington University, ComRAD consists of dual-polarized 1.4 GHz total-power radiometers (LH, LV) and a quad-polarized 1.25 GHz L band radar sharing a single parabolic dish antenna with a novel broadband stacked patch dual-polarized feed, a quad-polarized 4.75 GHz C band radar, and a single channel 10 GHz XHH radar. The instruments are deployed on a mobile truck with an 19-m hydraulic boom and share common control software; real-time calibrated signals, and the capability for automated data collection for unattended operation. Most microwave soil moisture retrieval algorithms developed for use at L band frequencies are based on the tau-omega model, a simplified zero-order radiative transfer approach where scattering is largely ignored and vegetation canopies are generally treated as a bulk attenuating layer. In this approach, vegetation effects are parameterized by tau and omega, the microwave vegetation opacity and single scattering albedo. One goal of our current research is to determine whether the tau-omega model can work for tree canopies given the increased scatter from trees compared to grasses and crops, and. if so, what are effective values for tau and omega for trees.

O'Neill, P.↗