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Non-DNA radiosensitive targets that initiate persistent behavioral deficits in rats exposed to space radiation

Predicting future CNS risks for astronauts during deep-space missions will rely substantially on ground-based rodent data with space-relevant ions and behaviors. For rats, the accumulated evidence indicates that less densely ionizing radiation, such as 4 He and 12 C ions, induce behavior deficits at lower doses than densely ionizing ions, such as 48 Ti and 56 Fe. However, this observation conflicts with standard somatic radiobiology, in which densely ionizing ions are generally more effective than less densely ionizing ions, and where the DNA/nucleus is the accepted target for radiation-induced tumorigenesis, cytogenetic aberrations, genetic mutations, and reproductive cell death. To gain deeper insight into the subcellular nature of the radiation targets for behavior risks, we compared the effects of dose, fluence, and linear energy transfer (LET) of 4 He and 56 Fe particles using existing datasets for four distinct behavioral outcomes in rats: elevated plus maze (EPM-anxiety), novel object recognition (NOR-memory), operant responding (OR-response to environmental stimuli), and attentional set-shifting (ATSET-cognitive flexibility). We confirmed that less densely ionizing particles (except protons) showed ~100-fold lower threshold doses than densely ionizing particles for behavioral deficits (0.1–1 cGy for 4 He vs. 15–100 cGy for 56 Fe). However, when analyzed by fluence the behavioral responses converged, indicating that 4 He and 56 Fe were equally effective on a per-track basis. When analyzed by LET, there were ~100-fold differences in the LET for maximum effectiveness for behavioral deficits and DNA endpoints (~1 vs ~100 keV/μm, respectively). These unique features of radiation-induced behavioral deficits (high sensitivity to particles in the 1-keV/μm range, insensitivity to protons in the 0.2 keV/μm range, and isofluence dependence for particles with LET>1 keV/μm) provide evidence in support of a new hypothesis of sub-micron sized radiosensitive targets for behavioral effects consistent with the thickness of plasma membranes and/or small subcellular structures, smaller than a whole synapse. Like our behavior findings, mouse immature oocyte killing which is known to have a plasma membrane target was also better explained by fluence, rather than dose. In contrast, fluence analyses for DNA/nuclear endpoints in somatic cells (e.g., tumor induction, chromosome aberrations) showed opposite results, suggesting that behavior targets are not DNA. Our findings raise questions regarding the identity of subcellular targets and the multi-cellular functional unit for behavior risks, low-dose susceptibility, and generalizability from rat to other species and astronauts.

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTAN↗

Risk of Adverse Cognitive or Behavioral Conditions and Psychiatric Disorders: Evidence Report

In April 2010, President Obama declared a space pioneering goal for the United States in general and NASA in particular. "Fifty years after the creation of NASA, our goal is no longer just a destination to reach. Our goal is the capacity for people to work and learn and operate and live safely beyond the Earth for extended periods of time, ultimately in ways that are more sustainable and even indefinite." Thus NASA's Strategic Objective 1.1 emerged as "expand human presence into the solar system and to the surface of Mars to advance exploration, science, innovation, benefits to humanity, and international collaboration" (NASA 2015b). Any space flight, be it of long or short duration, occurs in an extreme environment that has unique stressors. Even with excellent selection methods, the potential for behavioral problems among space flight crews remain a threat to mission success. Assessment of factors that are related to behavioral health can help minimize the chances of distress and, thus, reduce the likelihood of adverse cognitive or behavioral conditions and psychiatric disorders arising within a crew. Similarly, countermeasures that focus on prevention and treatment can mitigate the cognitive or behavioral conditions that, should they arise, would impact mission success. Given the general consensus that longer duration, isolation, and confined missions have a greater risk for behavioral health ensuring crew behavioral health over the long term is essential. Risk, which within the context of this report is assessed with respect to behavioral health and performance, is addressed to deter development of cognitive and behavioral degradations or psychiatric conditions in space flight and analog populations, and to monitor, detect, and treat early risk factors, predictors and other contributing factors. Based on space flight and analog evidence, the average incidence rate of an adverse behavioral health event occurring during a space mission is relatively low for the current conditions. While mood and anxiety disturbances have occurred, no behavioral emergencies have been reported to date in space flight. Anecdotal and empirical evidence indicate that the likelihood of an adverse cognitive or behavioral condition or psychiatric disorder occurring greatly increases with the length of a mission. Further, while cognitive, behavioral, or psychiatric conditions might not immediately and directly threaten mission success, such conditions can, and do, adversely impact individual and crew health, welfare, and performance.

Slack, Kelley J.↗

Rodent Habitat On ISS: Spaceflight Effects On Mouse Behavior

The NASA Decadal Survey (2011), Recapturing a Future for Space Exploration: Life and Physical Sciences Research for a New Era, emphasized the importance of expanding NASA life sciences research to long duration, rodent experiments on the International Space Station (ISS). To accomplish this objective, flight hardware, operations, and science capabilities supporting mouse studies in space were developed at NASA Ames Research Center. The first flight experiment carrying mice, Rodent Research Hardware and Operations Validation (Rodent Research-1), was launched on Sept 21, 2014 in an unmanned Dragon Capsule, SpaceX4, exposing the mice to a total of 37 days in space. Ground control groups were maintained in environmental chambers at Kennedy Space Center. Mouse health and behavior were monitored for the duration of the experiment via video streaming. Here we present behavioral analysis of two groups of five C57BL/6 female adult mice viewed via fixed camera views compared with identically housed Ground Controls. Flight (Flt) and Ground Control (GC) mice exhibited the same range of behaviors, including eating, drinking, exploratory behavior, self- and allo-grooming, and social interactions at similar or greater levels of occurrence. Mice propelled themselves freely and actively throughout the Habitat using their forelimbs to push off or by floating from one cage area to another, and they quickly learned to anchor themselves using tails and/or paws. Overall activity was greater in Flt as compared to GC mice, with spontaneous ambulatory behavior including the development of organized ‘circling’ or ‘race-tracking’ behavior that emerged within the first few days of flight and encompassed the primary dark cycle activity for the remainder of the experiment. We quantified the bout frequency, duration and rate of circling with respect to characteristic behaviors observed in the varying stages of the progressive development of circling: flipping utilizing two sides of the habitat, circling, multi-lap circling and group-circling. Once begun, mice did not regress to flipping behavior or other previous behavioral milestones for the remainder of flight. An overall upward trend in circling frequency, rate, duration, participation, and organization was observed over the course of the 37-day spaceflight experiment. In this presentation, we will summarize qualitative observations and quantitative comparisons of mice in microgravity and 1g conditions. Behavioral analyses provide important insights into the overall health and adaptation of mice to the space environment, and identify unique behaviors and social interactions to guide future habitat development and research on rodents in space.

Mouse↗

A Model-Based Approach to Engineering Behavior of Complex Aerospace Systems

One of the most challenging yet poorly defined aspects of engineering a complex aerospace system is behavior engineering, including definition, specification, design, implementation, and verification and validation of the system's behaviors. This is especially true for behaviors of highly autonomous and intelligent systems. Behavior engineering is more of an art than a science. As a process it is generally ad-hoc, poorly specified, and inconsistently applied from one project to the next. It uses largely informal representations, and results in system behavior being documented in a wide variety of disparate documents. To address this problem, JPL has undertaken a pilot project to apply its institutional capabilities in Model-Based Systems Engineering to the challenge of specifying complex spacecraft system behavior. This paper describes the results of the work in progress on this project. In particular, we discuss our approach to modeling spacecraft behavior including 1) requirements and design flowdown from system-level to subsystem-level, 2) patterns for behavior decomposition, 3) allocation of behaviors to physical elements in the system, and 4) patterns for capturing V&V activities associated with behavioral requirements. We provide examples of interesting behavior specification patterns, and discuss findings from the pilot project.

SysML↗

What’s That Supposed to Mean? Capturing Micro-Behaviors in Teams

Future long-duration space exploration (LDSE) crews will require extensive coordination, cooperation, and team functioning as they face a myriad of challenges rooted in both taskwork and teamwork (Bell et al., 2015; Landon et al., 2018). While exposed to extreme conditions, crew members must navigate living and working together in prolonged confinement. Moreover, astronaut teams are becoming increasingly diverse, introducing significant variability in team composition. This increasing diversity, alongside traditional constraints of LDSE, introduces additional challenges into effective team functioning. To date, most methods for capturing team functioning rely on self-report measures. Such measures are prone to several limitations, including but not limited to social desirability bias, halo effect, and leniency effects (Trull & Ebner-Priemer, 2013), which skew data and limit nuanced understandings of phenomena at play. Self-report measures broadly capture team functioning, lending the nature of such methods to identifying underlying “macro”-behaviors (i.e., behaviors that are long-standing and last over time). However, team functioning is far more complex than a series of macro-behaviors, rendering reliance on self-report data deficient for accurate measurement. Recent research demonstrates the potential of alternative methods for capturing team functioning, such as speech and physiological data (Chaffin et al., 2017; Murray & Oertel, 2018). Consequently, these methods are more suitable for capturing micro-behaviors: brief, often unconscious expressions that affect the extent to which an individual feels included by others around them (Paletz et al., 2013). Micro-behaviors can be further classified into micro-aggressions (i.e., subtle, negative exchanges; Keller & Galgay, 2010) or micro-affirmations (i.e., subtle, positive exchanges; Kyte et al. 2020), both of which influence team functioning. Due to the subtle nature of micro-behaviors, contextual factors have a significant impact when determining if it is aggressive or affirmative. Additionally, several iterations of micro-behaviors can have lingering effects on team interactions. For example, the use of “mm-hmm” by a crew member can function as both a micro-affirmation and micro-aggression. Specifically, it can be indication of active listening (i.e., micro-affirmation) or as an expression of annoyance (i.e., aggression) depending on the context in which it occurs. Auditory features (e.g., tone, frequency) can help delineate between the two forms; however, the contextual factors (e.g., previous interactions between team members, crew demographics) add a layer of complexity that render auditory features alone as insufficient to capture micro-behaviors. Consequently, this paper seeks to provide a novel approach in which multi-modal data (i.e., auditory features and contextual features) are used in a random-forest model to better identify distinguishing characteristics between micro-affirmations and micro-aggressions. In turn, detected micro-behaviors are used to predict team performance, thereby demonstrating the value of capturing micro-behaviors as supplemental data to macro-behaviors.

Sydney Begerowski↗

Can we use antipredator behavior theory to predict wildlife responses to high-speed vehicles?

Animals seem to rely on antipredator behavior to avoid vehicle collisions. There is an extensive body of antipredator behavior theory that have been used to predict the distance/time animals should escape from predators. These models have also been used to guide empirical research on escape behavior from vehicles. However, little is known as to whether antipredator behavior models are appropriate to apply to an approaching high-speed vehicle scenario. We addressed this gap by (a) providing an overview of the main hypotheses and predictions of different antipredator behavior models via a literature review, (b) exploring whether these models can generate quantitative predictions on escape distance when parameterized with empirical data from the literature, and (c) evaluating their sensitivity to vehicle approach speed using a simulation approach wherein we assessed model performance based on changes in effect size with variations in the slope of the flight initiation distance (FID) vs. approach speed relationship. The slope of the FID vs. approach speed relationship was then related back to three different behavioral rules animals may rely on to avoid approaching threats: the spatial, temporal, or delayed margin of safety. We used literature on birds for goals (b) and (c). Our review considered the following eight models: the economic escape model, Blumstein’s economic escape model, the optimal escape model, the perceptual limit hypothesis, the visual cue model, the flush early and avoid the rush (FEAR) hypothesis, the looming stimulus hypothesis, and the Bayesian model of escape behavior. We were able to generate quantitative predictions about escape distance with the last five models. However, we were only able to assess sensitivity to vehicle approach speed for the last three models. The FEAR hypothesis is most sensitive to high-speed vehicles when the species follows the spatial (FID remains constant as speed increases) and the temporal margin of safety (FID increases with an increase in speed) rules of escape. The looming stimulus effect hypothesis reached small to intermediate levels of sensitivity to high-speed vehicles when a species follows the delayed margin of safety (FID decreases with an increase in speed). The Bayesian optimal escape model reached intermediate levels of sensitivity to approach speed across all escape rules (spatial, temporal, delayed margins of safety) but only for larger (> 1 kg) species, but was not sensitive to speed for smaller species. Overall, no single antipredator behavior model could characterize all different types of escape responses relative to vehicle approach speed but some models showed some levels of sensitivity for certain rules of escape behavior. We derive some applied applications of our findings by suggesting the estimation of critical vehicle approach speeds for managing populations that are especially susceptible to road mortality. Overall, we recommend that new escape behavior models specifically tailored to high-speeds vehicles should be developed to better predict quantitatively the responses of animals to an increase in the frequency of cars, airplanes, drones, etc. they will face in the next decade.

54 ENVIRONMENTAL SCIENCES↗

Toward applied behavior analysis of life aloft

This article deals with systems at multiple levels, at least from cell to organization. It also deals with learning, decision making, and other behavior at multiple levels. Technological development of a human behavioral ecosystem appropriate to space environments requires an analytic and synthetic orientation, explicitly experimental in nature, dictated by scientific and pragmatic considerations, and closely approximating procedures of established effectiveness in other areas of natural science. The conceptual basis of such an approach has its roots in environmentalism which has two main features: (1) knowledge comes from experience rather than from innate ideas, divine revelation, or other obscure sources; and (2) action is governed by consequences rather than by instinct, reason, will, beliefs, attitudes or even the currently fashionable cognitions. Without an experimentally derived data base founded upon such a functional analysis of human behavior, the overgenerality of "ecological systems" approaches render them incapable of ensuring the successful establishment of enduring space habitats. Without an experimentally derived function account of individual behavioral variability, a natural science of behavior cannot exist. And without a natural science of behavior, the social sciences will necessarily remain in their current status as disciplines of less than optimal precision or utility. Such a functional analysis of human performance should provide an operational account of behavior change in a manner similar to the way in which Darwin's approach to natural selection accounted for the evolution of phylogenetic lines (i.e., in descriptive, nonteleological terms). Similarly, as Darwin's account has subsequently been shown to be consonant with information obtained at the cellular level, so too should behavior principles ultimately prove to be in accord with an account of ontogenetic adaptation at a biochemical level. It would thus seem obvious that the most productive conceptual and methodological approaches to long-term research investments focused upon human behavior in space environments will require multidisciplinary inputs from such wide-ranging fields as molecular biology, environmental physiology, behavioral biology, architecture, sociology, and political science, among others.

Review↗

Analysis of Adult Female Mouse (Mus musculus) Group Behavior on the International Space Station (ISS)

As interest in long duration effects of space habitation increases, understanding the behavior of model organisms living within the habitats engineered to fly them is vital for designing, validating, and interpreting future spaceflight studies. A handful of papers have previously reported behavior of mice and rats in the weightless environment of space. The Rodent Research Hardware and Operations Validation (Rodent Research-1; RR1) utilized the Rodent Habitat (RH) developed at NASA Ames Research Center to fly mice on the ISS (International Space Station). Ten adult (16-week-old) female C57BL/6 mice were launched on September 21st, 2014 in an unmanned Dragon Capsule, and spent 37 days in microgravity. Here we report group behavioral phenotypes of the RR1 Flight (FLT) and environment-matched Ground Control (GC) mice in the Rodent Habitat (RH) during this long-duration flight. Video was recorded for 33 days on the ISS, permitting daily assessments of overall health and well-being of the mice, and providing a valuable repository for detailed behavioral analysis. We previously reported that, as compared to GC mice, RR1 FLT mice exhibited the same range of behaviors, including eating, drinking, exploration, self- and allo-grooming, and social interactions at similar or greater levels of occurrence. Overall activity was greater in FLT as compared to GC mice, with spontaneous ambulatory behavior, including organized 'circling' or 'race-tracking' behavior that emerged within the first few days of flight following a common developmental sequence, and comprised the primary dark cycle activity persisting throughout the remainder of the experiment. Participation by individual mice increased dramatically over the course of the flight. Here we present a detailed analysis of 'race-tracking' behavior in which we quantified: (1) Complete lap rotations by individual mice; (2) Numbers of collisions between circling mice; (3) Lap directionality; and (4) Recruitment of mice into a group phenotype. This analysis contributes to the first NASA long-duration study of rodent behavior, providing evidence for the emergence of a distinctive, organized group behavior unique to the weightless space environment.

Behavior in Space↗

A simulation approach to estimate energy savings potential of occupant behavior measures

Occupant behavior in buildings is a leading factor influencing energy use in buildings. Low-cost behavioral solutions have demonstrated significant potential energy savings. Estimating the behavioral savings potential is important for a more effective design of behavior change interventions, which in turn will support more effective energy-efficiency policies. This study introduces a simulation approach to estimate the energy savings potential of occupant behavior measures. First it defines five typical occupant behavior measures in office buildings, then simulates and analyzes their individual and integrated impact on energy use in buildings. The energy performance of the five behavior measures was evaluated using EnergyPlus simulation for a real office building across four typical U.S. climates and two vintages. The Occupancy Simulator was used to simulate the occupant movement in each zone with inputs from the site survey of the case building. Based on the simulation results, the occupant behavior measures can achieve overall site energy savings as high as 22.9% for individual measures and up to 41.0% for integrated measures. Although energy savings of behavior measures would vary depending upon many factors, the presented simulation approach is robust and can be adopted for other studies aiming to quantify occupant behavior impact on building performance.

29 ENERGY PLANNING, POLICY, AND ECONOMY↗

Deer Vigilance and Movement Behavior Are Affected by Edge Density and Connectivity

ABSTRACT Animal behavior is an important component of individual, population, and community responses to anthropogenic habitat alteration. For example, antipredator behavior (e.g., vigilance) and animal movement behavior may both be important behavioral responses to the increased density of habitat edges and changes in patch connectivity that characterize highly modified habitats. Importantly, edge density and connectivity might interact, and this interaction is likely to mediate animal behavior: linear, edge‐rich landscape features often provide structural connectivity between patches, but the functional connectedness of patches for animal use could depend upon how edge density modifies animal vigilance and movement. Using remote cameras in large‐scale experimental landscapes that manipulate edge density (high‐ vs. low‐density edges) and patch connectivity (isolated or connected patches), we examined the effects of edge density and connectivity on the antipredator behavior and movement behavior of white‐tailed deer ( Odocoileus virginianus ). Deer vigilance was 1.38 times greater near high‐density edges compared to low‐density edges, regardless of whether patches were connected or isolated. Deer were also more likely to move parallel to connected high‐density edges than all other edge types, suggesting that connectivity promotes movement along high‐density edges. These results suggest that increases in edge density that accompany human fragmentation of existing habitats may give rise to large‐scale changes in the antipredator behavior of deer. These results also suggest that conservation strategies that simultaneously manipulate edge density and connectivity (i.e., habitat corridors) may have multiple effects on different aspects of deer behavior: linear habitat corridors were areas of high vigilance, but also areas where deer movement behavior implied increased movement along the habitat edge.

Bartel, Savannah L. [University of Wisconsin‐Madis↗

Towards Behavioral Reflexion Models

Software architecture has become essential in the struggle to manage today s increasingly large and complex systems. Software architecture views are created to capture important system characteristics on an abstract and, thus, comprehensible level. As the system is implemented and later maintained, it often deviates from the original design specification. Such deviations can have implication for the quality of the system, such as reliability, security, and maintainability. Software architecture compliance checking approaches, such as the reflexion model technique, have been proposed to address this issue by comparing the implementation to a model of the systems architecture design. However, architecture compliance checking approaches focus solely on structural characteristics and ignore behavioral conformance. This is especially an issue in Systems-of- Systems. Systems-of-Systems (SoS) are decompositions of large systems, into smaller systems for the sake of flexibility. Deviations of the implementation to its behavioral design often reduce the reliability of the entire SoS. An approach is needed that supports the reasoning about behavioral conformance on architecture level. In order to address this issue, we have developed an approach for comparing the implementation of a SoS to an architecture model of its behavioral design. The approach follows the idea of reflexion models and adopts it to support the compliance checking of behaviors. In this paper, we focus on sequencing properties as they play an important role in many SoS. Sequencing deviations potentially have a severe impact on the SoS correctness and qualities. The desired behavioral specification is defined in UML sequence diagram notation and behaviors are extracted from the SoS implementation. The behaviors are then mapped to the model of the desired behavior and the two are compared. Finally, a reflexion model is constructed that shows the deviations between behavioral design and implementation. This paper discusses the approach and shows how it can be applied to investigate reliability issues in SoS.

Ackermann, Christopher↗

What’s That Supposed to Mean? Capturing Micro-Behaviors in Teams

Future long-duration space exploration (LDSE) crews will require extensive coordination, cooperation, and team functioning as they face a myriad of challenges rooted in both taskwork and teamwork (Bell et al., 2015; Landon et al., 2018). While exposed to extreme conditions, crew members must navigate living and working together in prolonged confinement. Moreover, astronaut teams are becoming increasingly diverse, introducing significant variability in team composition. This increasing diversity, alongside traditional constraints of LDSE, introduces additional challenges into effective team functioning. To date, most methods for capturing team functioning rely on self-report measures. Such measures are prone to several limitations, including but not limited to social desirability bias, halo effect, and leniency effects (Trull & Ebner-Priemer, 2013), which skew data and limit nuanced understandings of phenomena at play. Self-report measures broadly capture team functioning, lending the nature of such methods to identifying underlying “macro”-behaviors (i.e., behaviors that are long-standing and last over time). However, team functioning is far more complex than a series of macro-behaviors, rendering reliance on self-report data deficient for accurate measurement. Recent research demonstrates the potential of alternative methods for capturing team functioning, such as speech and physiological data (Chaffin et al., 2017; Murray & Oertel, 2018). Consequently, these methods are more suitable for capturing micro-behaviors: brief, often unconscious expressions that affect the extent to which an individual feels included by others around them (Paletz et al., 2013). Micro-behaviors can be further classified into microaggressions (i.e., subtle, negative exchanges; Keller & Galgay, 2010) or micro-affirmations (i.e., subtle, positive exchanges; Kyte et al. 2020), both of which influence team functioning. Due to the subtle nature of micro-behaviors, contextual factors have a significant impact when determining if it is aggressive or affirmative. Additionally, several iterations of microbehaviors can have lingering effects on team interactions. For example, the use of “mm-hmm” by a crew member can function as both a micro-affirmation and micro-aggression. Specifically, it can be indication of active listening (i.e., micro-affirmation) or as an expression of annoyance (i.e., aggression) depending on the context in which it occurs. Auditory features (e.g., tone, frequency) can help delineate between the two forms; however, the contextual factors (e.g., previous interactions between team members, crew demographics) add a layer of complexity that render auditory features alone as insufficient to capture micro-behaviors. Consequently, this paper seeks to provide a novel approach in which multi-modal data (i.e., auditory features and contextual features) are used in a random-forest model to better identify distinguishing characteristics between micro-affirmations and micro-aggressions. In turn, detected micro-behaviors are used to predict team performance, thereby demonstrating the value of capturing micro-behaviors as supplemental data to macro-behaviors.

Sydney R. Begerowski↗

A Preliminary Study on the Feasibility of Large Language Models for Detecting Micro-Behaviors Among Team Members in Space Missions

Large-language models (LLMs) have been recently used for spoken language understanding (SLU) to infer meaning and semantics from speech in tasks such as speaker intent and sentiment classification. Due to being trained on large amounts of data, and their ability to understand context and relationships between words, LLMs are competent, enabling them to generalize across tasks without requiring many task-specific training samples. This research examines the feasibility of few-shot learning in LLMs for detecting subtle, brief, and possibly unconscious interactions between team members, called ``micro-behaviors," and provides insights into the appropriate design of LLMs for this task. Our data came from 5 teams participating in a 45-day mission at the US National Aeronautics and Space Administration’s (NASA) Human Exploration Research Analog (HERA). More specifically we used data collected from team interaction battery (TIB) tasks teams performed five times in-mission which comprise an average 1.5 hours of conversation data per day. Micro-behaviors were coded according to an adapted version of Smith & Griffins (2022) theoretical framework in terms of Violation (i.e., presence of valenced behavior, uplifting/positive or discouraging/negative), Intensity (i.e., force of behavior in terms of how uplifting or discouraging is the behavior), and Intent (i.e., motive of the behavior in terms of whether it was deliberate or unintentional). We explore the ability of LLMs to detect the presence and intensity of micro-behaviors. We examine employing and fine-tuning readily available LLMs (i.e., RoBERTa, DistilBERT), as well as prompting state-of-the-art sequence classification models (i.e., Llama-2, Llama-3). In a total of 13,058 conversational turns (17.8% uplifting, 3.3% discouraging, 75.76% neutral, 3.14% nulls), we compute the macro F1-score of the 3-way micro-behavior classification task (i.e., classifying among uplifting, discouraging, and neutral; 33% chance). Results indicate that the RoBERTa model achieves a F1-score of 36.2% (uplift: 43.3% precision (P), 15.1% recall (R); discourage: 20% P, 0.5% R). These results significantly improve when we augment the data via paraphrasing in the RoBERTa model, reaching a 41.2% macro F1-score (uplift: 37.7% P, 86.3% R; discourage: 3.5% P, 1.8% R). Finally, the Llama-2 model with 3-shot prompting yields 38% macro F1-score (uplift: 28.7% P, 20% R; discourage: 7.2% P, 18% R), which is slightly better compared to the RoBERTa model without data augmentation, highlighting the effectiveness of sequence classification models in detecting minority classes with a small sample size. Findings indicate that LLMs hold potential to detect subtle behaviors in conversations, which could be valuable in assessing team behavior in space exploration missions. Future studies will evaluate the performance of different LLM prompting strategies or fine-tuning methods.

Ankush Raut↗

A meta-analysis of the association between gender and protective behaviors in response to respiratory epidemics

Respiratory infectious disease epidemics and pandemics are recurring events that levy a high cost on individuals and society. The health-protective behavioral response of the public plays an important role in limiting respiratory infectious disease spread. Health-protective behaviors take several forms. Behaviors can be categorized as pharmaceutical (e.g., vaccination uptake, antiviral use) or non-pharmaceutical (e.g., hand washing, face mask use, avoidance of public transport). Due to the limitations of pharmaceutical interventions during respiratory epidemics and pandemics, public health campaigns aimed at limiting disease spread often emphasize both non-pharmaceutical and pharmaceutical behavioral interventions. Understanding the determinants of the public’s behavioral response is crucial for devising public health campaigns, providing information to parametrize mathematical models, and ultimately limiting disease spread. While other reviews have qualitatively analyzed the body of work on demographic determinants of health-protective behavior, this meta-analysis quantitatively combines the results from 85 publications to determine the global relationship between gender and health-protective behavioral response. The results show that women in the general population are about 50% more likely than men to adopt/practice non-pharmaceutical behaviors. Conversely, men in the general population are marginally (about 12%) more likely than women to adopt/practice pharmaceutical behaviors. It is possible that factors other than pharmaceutical/non-pharmaceutical status not included in this analysis act as moderators of this relationship. We find these results suggest an inherent difference in how men and women respond to epidemic and pandemic respiratory infectious diseases. In conclusion, this information can be used to target specific groups when developing non-pharmaceutical public health campaigns and to parameterize epidemic models incorporating demographic information.

60 APPLIED LIFE SCIENCES↗

Effect of environmental enrichment devices on behaviors of single- and group-housed squirrel monkeys (Saimiri sciureus)

Squirrel monkeys display an interest in novel places, habituate to new situations, and spend most of their daily activity in the wild in large groups engaging in feeding behaviors over a broad area. Captivity limits these behaviors and consequently may disrupt normal social organizations. In captivity, squirrel monkeys may exhibit stereotypical behaviors that are believed to indicate decreased psychologic well-being. When a monkey's behavior can be made to approach that seen in the wild, and stereotypical behaviors are minimal, it is assumed that psychologic well-being is adequate. Environmental enrichment devices have been used to address the Animal Welfare Act requirement that psychologic well-being of captive nonhuman primates be considered. The purpose of the study reported here was to examine whether various environmental enrichment devices improve the psychologic well-being of captive squirrel monkeys. In the study, we used behavioral observation to quantify the effectiveness of several environmental enrichment devices for reducing stereotypical behaviors in squirrel monkeys housed alone or in groups. Analysis of our results revealed that the environmental enrichment devices did not affect the expression of normal or stereotypical behaviors, but that the type of housing did.

NASA Discipline Neuroscience↗

A framework for quantifying the impact of occupant behavior on energy savings of energy conservation measures

To improve energy efficiency—during new buildings design or during a building retrofit—evaluating the energy savings potential of energy conservation measures (ECMs) is a critical task. In building retrofits, occupant behavior significantly impacts building energy use and is a leading factor in uncertainty when determining the effectiveness of retrofit ECMs. Current simulation-based assessment methods simplify the representation of occupant behavior by using a standard or representative set of static and homogeneous assumptions ignoring the dynamics, stochastics, and diversity of occupant's energy-related behavior in buildings. The simplification contributes to significant gaps between the simulated and measured actual energy performance of buildings. This paper presents a framework for quantifying the impact of occupant behaviors on ECM energy savings using building performance simulation. During the first step of the study, three occupant behavior styles (austerity, normal, and wasteful) were defined to represent different levels of energy consciousness of occupants regarding their interactions with building energy systems (HVAC, windows, lights and plug-in equipment). Next, a simulation workflow was introduced to determine a range of the ECM energy savings. Then, guidance was provided to interpret the range of ECM savings to support ECM decision making. Finally, a pilot study was performed in a real building to demonstrate the application of the framework. Simulation results show that the impact of occupant behaviors on ECM savings vary with the type of ECM. Occupant behavior minimally affects energy savings for ECMs that are technology-driven (the relative savings differ by less than 2%) and have little interaction with the occupants; for ECMs with strong occupant interaction, such as the use of zonal control variable refrigerant flow system and natural ventilation, energy savings are significantly affected by occupant behavior (the relative savings differ by up to 20%). Finally, the study framework provides a novel, holistic approach to assessing the uncertainty of ECM energy savings related to occupant behavior, enabling stakeholders to understand and assess the risk of adopting energy efficiency technologies for new and existing buildings.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗

Data-driven occupant-behavior analytics for residential buildings

Many advances have been made in building technology to help save energy, but influencing the behavior of the occupants is still necessary to achieve low-energy use targets. One of the most practical ways to influence and change occupant behaviors is through incentives. Developing incentives for energy-saving and quantifying the impact of occupant behaviors are both active areas of research. Here, we propose a data analytics framework for detecting changes in occupant behaviors, which will help build an analytics feedback loop from behavior impact to incentive design. The framework has two major parts. The first forecasts energy consumption for each occupant, while the second determines a probability distribution for changes in energy consumption. The parts are interchangeable with other existing machine learning and statistical methods. A specific instantiation of the framework, using kernel ridge-regression for forecasting and k-means to find an empirical behavior distribution, is described in detail. An HVAC use-case with 5 different incentivized behaviors is used as an example to show that the framework can detect behavior changes induced by incentives. Furthermore, we show that some simpler behavior-change detection methods do not work, further justifying the use of advanced analytics.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗

The Effects of Placental-expanded (PLX-PAD) Stromal Cell Treatment, Hindlimb Unloading, and Isolation on the Behavior of Female Mice

Spaceflight can lead to altered immune responses and inflammation (Crucian et al. 2014) and elevated levels of inflammation are connected to anxiety and depression. A recent study on the International Space Station showed that mice exhibited a novel circling behavior during spaceflight (Ronca et al. 2019). However, there is still a gap in knowledge on how microgravity impacts behavior. In this current study, we performed 30 days of hindlimb unloading (HU) on four-month old female mice and analyzed select behavior from video image capture. We also determined the effects of PLacental-eXpanded stromal cells derived from the maternal placenta (PLX-PAD), alone and in combination with HU and isolation, on behavior. We have previously shown that PLX-PAD mitigates select inflammatory responses and changes in cytokine expression caused by HU. In-cage behaviors were analyzed in HU or control female mice treated with 2 injections of PlasmaLyte (Sham) or PLX-PAD (n=7/group). We found that PLX-PAD decreased exploratory behaviors compared to Sham-treated mice at night. Normally loaded (NL) PLX mice slept less than NL Sham mice during the day, and HU animals had significantly higher involuntary movement during sleep compared to NL animals, suggesting sleep disruption. Overall, we show that both HU and PLX affect important behavioral factors. This experiment is the first to study the effects of PLX-PAD on behavior. Additional studies are needed to define the behavioral changes in spaceflight and test possible countermeasures.

PLacental-EXpanded stromal cells, behavior, microg↗