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At least 307 records · Page 17
All sourcesNASA NTRSDOE OSTI
DOE OSTI2020

Applications of Hyporheic Zone Sampling for Assessing VOC Plume Engineered Remediation and Natural Attenuation Effectiveness - 20414

Determining the efficacy of engineered remedial actions and/or natural attenuation as an acceptable EPA Record of Decision (ROD) must address long-term temporal and spatial variability. Since final plume downslope destinations are aquifers and related surface streams and rivers, hyporheic zone sampling is essential. Comprehensive hyporheic sampling documents plume groundwater remediation prior to upwelling into stream benthic strata and natural attenuation within this dynamic zone itself. Our intensive sampling of the Savannah River Site (SRS) Chemical, Metals, and Pesticides (CMP) Pits VOC plume-fringe from 2005 to 2018 documented PCE natural attenuation and effectiveness of In Situ Thermal Treatment (ISTT). Most plume hyporheic upwelling was delimited to a 20 m inflow reach along Pen Branch. Our study utilized corroborative sampling methodologies: ambient hole-water grab samples: passive diffusion bags (PDB); and hole-core sediment samples to confirm station to station spatial and temporal trends. VOC hot spot detections were confirmed from both groundwater and soil core samples. VOC pathways through the Pen Branch hyporheic zone were assessed at hole-installation using sequential soil samples from augered cores and grab-samples of hole-bottom water. Longer term, composite water samples were collected from PDBs deployed for a minimum two-week equilibration period. Soil samples were collected from the 15 and 65 cm core levels in December 2017 and June 2018 at six stations. Additional core depths of 40 and 85 cm were sampled in June 2018 at the highest VOC station (5DB80). All samples were analyzed in an EPA certified lab by purge and trap GC-MS. Effectiveness of previous ISTT and soil vapor extraction (SVE) for this CMP Pits PCE plume, was clearly documented by significant downward trends in VOC at hyporheic zone stations prior to December 2015. Achieving final stage conversions of PCE daughter products to ethene and CO{sub 2} may be limited depending upon suitable interactions between hyporheic zone sediment morphology, hydrology, and redox conditions. Because declines in VOC daughter products at our hyporheic stations may also be caused by physical actions of dilution and dispersion rather than true biochemical dechlorination, full quantification of natural attenuation requires the use of compound-specific isotope ratio analysis (CSIA) and microbial 16S rRNA with qPCR. To assist these next-phase efforts, a natural attenuation index (NAI) was used to help produce quicker, more cost-effective decision making for locating CSIA and microbial sampling efforts. The NAI used natural logarithm transformed GC-MS results for station VOC degradation compounds versus VOC parent compounds. Wide differences were observed between stations. (authors)

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗
DOE OSTI2023

Evaluation of a Wave Powered Water Pump Performance by Ocean Field Testing and WEC-Sim Modeling

This submission from AMEC (the Atlantic Marine Energy Center) includes data from an ocean field deployment of a wave powered water pump in March 2023. The wave pump is an upweller device, designed to enhance macroalgal aquaculture. The wave pump device was deployed off the coast of Isles of Shoals Appledore Island in Maine, USA. The data were collected using a custom-built DAQ module comprised of Arduino Unos. GPS time stamp accompanies the data. The data are volumetric flow rate from the wave pump, and relative motion of the device between float and spar buoys. Flow rate is measured by flow meter, and relative motion is measured by lidar. Calibration data for the lidar and flow meter sensors are included. This data set also includes synchronous Sofar Spotter buoy data from a mooring approximately 300 feet away from the wave pump mooring. Video data from the deployment are included from both on-board the device sporadically throughout the deployment, and from a webcam for a short duration of the deployment. Hydrophone data were also taken co-currently, and are available by contacting Martin Wosnik at the University of New Hampshire. The Matlab code used to process the field data is incorporated. A biological assessment is included which aided the NEPA consultation process, prior to conducting the field deployment. A WEC-Sim numerical model of the wave pump, and a re-design effort are part of this work. Code used to validate the WEC-Sim model from the field data are also included.

16 TIDAL AND WAVE POWER↗
DOE OSTI2025

Surface Energy Balance System (SEBS) Instrument Handbook

A Surface Energy Balance System (SEBS) has been installed collocated with each deployed ECOR system at the Southern Great Plains (SGP), North Slope of Alaska (NSA), Tropical Western Pacific (TWP), ARM Mobile Facility 1 (AMF1), and ARM Mobile Facility 2 (AMF2). The surface energy balance system consists of upwelling and downwelling solar and infrared radiometers within one net radiometer, a wetness sensor, and soil measurements. The SEBS measurements allow the comparison of ECOR sensible and latent heat fluxes with the energy balance determined from the SEBS and provide information on wetting of the sensors for data quality purposes.

54 ENVIRONMENTAL SCIENCES↗
NASA NTRS1998

The Climatological Annual Cycle of Satellite-derived Phytoplankton Pigments in the Alboran Sea: A Physical Interpretation

The circulation and upwelling processes (coastal and gyre-induced) that control the phytoplankton distribution in the Alboran sea are examined by analyzing monthly climatological patterns of Coastal Zone Color Scanner (CZCS) pigment concentrations, sea surface temperatures, winds, and seasonal geostrophic fields.

Alboran sea phytoplankton distribution coastal upw↗
NASA NTRS2008

A Three-Dimensional Variational Data Assimilation Scheme for the Regional Ocean Modeling System: Implementation and Basic Experiments

A three-dimensional variational data assimilation scheme for the Regional Ocean Modeling System (ROMS), named ROMS3DVAR, has been described in the work of Li et al. (2008). In this paper, ROMS3DVAR is applied to the central California coastal region, an area characterized by inhomogeneity and anisotropy, as well as by dynamically unbalanced flows. A method for estimating the model error variances from limited observations is presented, and the construction of the inhomogeneous and anisotropic error correlations based on the Kronecker product is demonstrated. A set of single observation experiments illustrates the inhomogeneous and anisotropic error correlations and weak dynamic constraints used. Results are presented from the assimilation of data gathered during the Autonomous Ocean Sampling Network (AOSN) experiment during August 2003. The results show that ROMS3DVAR is capable of reproducing complex flows associated with upwelling and relaxation, as well as the rapid transitions between them. Some difficulties encountered during the experiment are also discussed.

California coastal ocean↗
DOE OSTI2020

Impact of UV-light and pH on the Fate of Tc, I, and U in Wetlands at Savannah River Site - 20230

The Savannah River Site (SRS) is one of the major nuclear facilities owned by the U.S Department of Energy. During the Cold War, these facilities produced large amounts of radioactive and hazardous waste. On site, three unlined seepage basins in the F-Area received approximately 1.8 billion gallons of low-level waste containing nitric acid, radionuclides, and dissolved metals due to plutonium and tritium production operations. The acidic nature of the waste created a source of relatively mobile radionuclides below the basins. Radionuclides previously disposed of within the F-Area, including uranium isotopes (U), technetium-99 (Tc-99), and iodine-129 (I-129), are moving with groundwater towards Four Mile Branch Wetland, where they are subsequently upwelling and interacting with natural organic matter (NOM). Many environmental factors including redox conditions, porewater composition, pH, light, and temperature affect the degradation of organic matter as well as interactions with Tc-99, U, and I-129. In particular, the high concentrations of nitrate from the acidic waste may increase the formation of reactive oxygen species (ROS) that impact both degradation of NOM and behavior of contaminants. In the presence of sunlight, I-129, Tc-99, and U speciation and complexation behavior may be affected by ROS and NOM degradation products in addition to the factors generally considered in subsurface systems in the absence of light. This research aims to determine whether the interactions between radionuclides, NOM, and nitrate affect the fate of I-129, Tc-99, and U and which processes are controlling their behavior. Experiments were conducted at variable pH in the presence of NaNO{sub 3} in order to determine the impact of light and pH on NOM degradation and to evaluate the impact on the fate of contaminants of concern. Soil samples high in NOM collected from two sites in the Southeastern United States (Savannah River Site and the Everglades) were studied. Batch experiments were conducted with NOM and the following aqueous contaminants, U, I-129, Tc-99 with exposure to ultraviolet (UV) light in an environmental chamber. This study was conducted in a sterile environment in order to exclude the potential for microbial degradation of organic matter. Results indicated photodegradation of NOM and significant interaction of radionuclides with NOM. (authors)

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗
DOE OSTI2024

Potential of U.S. Marine and Offshore Wind Energy to Power Marine Carbon Dioxide Removal

The goal of this project was to provide a preliminary feasibility assessment of powering different marine carbon dioxide removal (mCDR), carbon capture (mCC), and carbon sequestration (mCS) strategies with marine energy. The investigation focused on methods with clear power requirements at sea beyond monitoring such as artificial upwelling, deep ocean storage, electrochemical (eChem) mCDR and mCC, offshore microalgae cultivation, and seaweed farming and sinking. The project found that eChem mCDR powered by marine energy and offshore wind energy available in the United States could meet global CDR scales needed by 2040 and 2050 to limit warming to 1.5 degrees C by 2100 and marine energy alone could greatly contribute to reaching scales needed by 2040. Note that this preliminary estimate assumes that it is possible to harvest all the marine and offshore wind resources available in the United States with existing technology options. Though the biological methods were limited in scale they still hold promise in developing carbon negative fuels and products which can reduce emissions in the short term.

afforestation↗
DOE OSTI2020

Population genomic response to geographic gradients by widespread and endemic fishes of the Arabian Peninsula

Genetic structure within marine species may be driven by local adaptation to their environment, or alternatively by historical processes, such as geographic isolation. The gulfs and seas bordering the Arabian Peninsula offer an ideal setting to examine connectivity patterns in coral reef fishes with respect to environmental gradients and vicariance. The Red Sea is characterized by a unique marine fauna, historical periods of desiccation and isolation, as well as environmental gradients in salinity, temperature, and primary productivity that vary both by latitude and by season. The adjacent Arabian Sea is characterized by a sharper environmental gradient, ranging from extensive coral cover and warm temperatures in the southwest, to sparse coral cover, cooler temperatures, and seasonal upwelling in the northeast. Reef fish, however, are not confined to these seas, with some Red Sea fishes extending varying distances into the northern Arabian Sea, while their pelagic larvae are presumably capable of much greater dispersal. These species must therefore cope with a diversity of conditions that invoke the possibility of steep clines in natural selection. Here, we test for genetic structure in two widespread reef fish species (a butterflyfish and surgeonfish) and eight range-restricted butterflyfishes across the Red Sea and Arabian Sea using genome-wide single nucleotide polymorphisms. We performed multiple matrix regression with randomization analyses on genetic distances for all species, as well as reconstructed scenarios for population subdivision in the species with signatures of isolation. We found that (a) widespread species displayed more genetic subdivision than regional endemics and (b) this genetic structure was not correlated with contemporary environmental parameters but instead may reflect historical events. We propose that the endemic species may be adapted to a diversity of local conditions, but the widespread species are instead subject to ecological filtering where different combinations of genotypes persist under divergent ecological regimes.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2023

Juvenile life history diversity is associated with lifetime individual heterogeneity in a migratory fish

Abstract Differences in the life history pathways (LHPs) of juvenile animals are often associated with differences in demographic rates in later life stages. For migratory animals, different LHPs often result in animals from the same population occupying distinct habitats subjected to different environmental drivers. Understanding how demographic rates differ among animals expressing different LHPs may reveal fitness trade‐offs that drive the expression of alternative LHPs and enable better prediction of population dynamics in a changing environment. To understand how demographic outcomes and their relationships with environmental variables differ among animals with different LHPs, we analyzed a long‐term (2006–2021) mark–recapture dataset for Chinook salmon ( Oncorhynchus tshawytscha ) from the Wenatchee River, Washington, USA. Distinct LHPs represented in this population include either remaining in the natal stream until emigrating to the ocean as a 1‐year‐old (natal‐reach rearing) or emigrating from the natal stream and rearing in downstream habitats for several months before completing the emigration to the ocean as a 1‐year‐old (downstream rearing). We found that downstream‐rearing fish emigrated to the ocean 19 days earlier on average and returned as adults from the ocean at higher rates. We detected a positive correlation between rate of return from the ocean by downstream‐rearing fish and coastal upwelling in their spring of outmigration, whereas for natal‐reach‐rearing fish we detected a positive correlation with sea surface temperature during their first marine summer. Different responses to environmental variability should lead to asynchrony in adult abundance among juvenile LHPs. A higher proportion of downstream‐rearing fish returned at younger ages compared with natal‐reach‐rearing fish, which contributed to variability in age at reproduction and greater mixing across generations. Our results demonstrate how diversity in juvenile LHPs is associated with heterogeneity in demographic rates during subsequent life stages, which can in turn affect variance in aggregate population abundance and response to environmental change. Our findings underscore the importance of considering life history diversity in demographic analyses and provide insights into the effects of life history diversity on population dynamics and trade‐offs that contribute to the maintenance of life history diversity.

Sorel, Mark H.↗
DOE OSTI2022

Metatranscriptomics reveals a shift in microbial community composition and function during summer months in a coastal marine environment

Abstract Temperate coastal marine waters are often thermally stratified from spring through fall but can be dynamic and disrupted by tidal currents and wind‐driven upwelling. These mixing events introduce deeper, cooler water with a higher partial pressure of CO 2 (pCO2) and its associated microbial communities to the surface. Anecdotally, these events impact shellfish hatcheries and farms, warranting improved understanding of changes in composition and activity of marine microbial communities in relation to environmental processes. To characterize both compositional and functional changes associated with abiotic factors, here, we generate a reference metatranscriptome from the Strait of Georgia over representative seasons and analyze metatranscriptomic profiles of the microorganisms present within intake water containing different pCO 2 levels at a shellfish hatchery in British Columbia from June through October. Abiotic factors studied include pH, temperature, alkalinity, aragonite, calcite, and pCO 2 . Community composition changes were observed to occur at broad taxonomic levels and most notably to vary with temperature and pCO 2 . Functional gene expression profiles indicated a strong difference between early (June–July) and late summer (August–October) associated with viral activity. The taxonomic data suggest this could be due to the termination of cyanobacteria and phytoplankton blooms by viral lysis in the late season. Functional analysis indicated fewer differentially expressed transcripts associated with abiotic variables (e.g., pCO 2 ) than with the temporal effect. Microbial composition and activity in these waters vary with both short‐term effects observed alongside abiotic variation and long‐term effects observed across seasons. The analysis of both taxonomy and functional gene expression simultaneously in the same samples by environmental RNA (eRNA metatranscriptomics) provided a more comprehensive view for monitoring water bodies than either would in isolation.

Sutherland, Ben J. G.↗
DOE OSTI2023

Thermal insulation versus capacitance: A simulation experiment comparing effects of shade and hyporheic exchange on daily and seasonal stream temperature cycles

In streams where water temperatures stress native biota, management of riparian shade or hyporheic exchange are both considered viable management strategies for reducing the peaks of daily and seasonal stream channel temperature cycles. Although shade and hyporheic exchange may have similar effects on stream temperatures, their mechanisms differ. Improved understanding of the heat-exchange mechanisms influenced by shade and hyporheic exchange will aid in the appropriate application of either stream temperature management strategy. To illustrate a conceptual model highlighting shade as ‘thermal insulation’ and hyporheic exchange imparting ‘thermal capacitance’ to a stream reach, we conducted an in-silico simulation modelling experiment increasing shade or hyporheic exchange parameters on an idealized, hypothetical stream. We assessed the potential effects of increasing shade or hyporheic exchange on a stream reach using an established process-based heat-energy budget model of stream-atmosphere heat exchange and incorporated an advection-driven hyporheic heat exchange routine. The model tracked heat transport through the hyporheic zone and exchange with the stream channel, while including the effects of hyporheic water age distribution on upwelling hyporheic temperatures. Results showed that shade and hyporheic exchange similarly damped diurnal temperature cycles and differentially altered seasonal cycles of our theoretical stream. In winter, hyporheic exchange warmed simulated channel temperatures whereas shade had little effect. In summer, both shade and hyporheic exchange cooled channel temperatures, though the effects of shade were more pronounced. Our simple-to-grasp analogies of ‘thermal insulation’ for shade effects and ‘thermal capacitance’ for hyporheic exchange effects on stream temperature encourage more accurate conceptualization of complex, dynamic heat exchange processes among the atmosphere, stream channel, and alluvial aquifer.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2025

Improved Simulation of East Asian Summer Monsoon in the High-resolution CESM1 and Its Causes

Based on the high- and low-resolution Community Earth System Model, version 1 (CESM1), and corresponding simulations from phase 6 of the Coupled Model Intercomparison Project (CMIP6), we compare the interannual variability of the East Asian summer monsoon (EASM). The EASM interannual variability is characterized by the anomalous western North Pacific anticyclone (WNPAC) circulation and the dipole rainfall pattern with a negative southern lobe over the western North Pacific and a positive northern lobe along the Meiyu–Baiu region, which is better reproduced by the high-resolution models. Here, the reason for the improvement in the high-resolution models has been attributed to the better simulation of the warm temperature advection from the wind anomalies on the climatological temperature gradient. Positive sea surface temperature (SST) anomalies over the tropical Indian Ocean are the key to the improved wind anomalies featuring a WNPAC in the high-resolution models. The warm SST anomalies over the tropical Indian Ocean strengthen the WNPAC by triggering a Kelvin-wave response to the enhanced heat release induced by the increased precipitation. Based on the mixed-layer heat budget analysis, the warm SST anomalies over the western Indian Ocean in the high-resolution CESM1 are tied to the anomalous easterly wind along the equator, which reduces surface evaporation and upwelling. Therefore, the better simulations of air-sea feedback and the oceanic mesoscale eddy over the western Indian Ocean are the key for the improved simulation of the EASM interannual variations in the high-resolution CESM1.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2023

Revisiting the equatorial Pacific sea surface temperature response to global warming

The relative roles of the oceanic and atmospheric processes in the pattern formation of the equatorial Pacific sea surface temperature (SST) response to global warming is investigated using a set of climate model experiments embedded with a novel partial coupling technique. Here, the modeling results show that the SST response experiences a transition from a La Niña-like warming pattern at the initial stage to an El Niño-like warming pattern at the quasi-equilibrium stage. By decomposing anomalous equatorial Pacific SST into atmosphere-forced passive component and ocean dynamics-induced active component, it is found that the SST warming pattern at both stages is entirely induced by its active component. Specifically, the meridional and vertical ocean circulation changes play a dominant role in forming the La Niña-like SST warming pattern at the initial stage, and the zonal and meridional ocean circulation changes are responsible for the formation of the El Niño-like SST warming pattern at the quasi-equilibrium stage. In contrast, the passive SST at both stages is characterized by a zonally uniform warming along the equator, which can be explained by a balance between the cooling effect associated with mean upwelling and the warming effect due to surface passive heat flux change. In addition, this study finds that it is the slowdown of the Pacific subtropical cells during the transition period that controls the evolution of the equatorial SST warming pattern by changing the meridional and vertical ocean heat transports.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2020

Seismic identification of geothermal prospecting in Harrat Rahat, Northern Arabian Shield

Harrat Rahat volcanic field lies along the Makkah-Madinah-Nafud active volcanic line. It has extensive linear weak zones of wrench faults that could provide ideal conduits for upwelling magma and hydrothermal fluids as well. Here, passive seismic data recorded by seismic stations installed within and around Harrat Rahat has been processed for the detection of microseismic events. Moreover, ambient noise was also gathered and analyzed to construct a subsurface velocity model. This model indicates low velocity zone in Harrat Rahat, and two areas with coincident basement faults and possibly with enhanced subsurface temperatures were identified. It is concluded that Harrat Rahat has high geothermal potential and promising for geothermal renewable power.

58 GEOSCIENCES↗
DOE OSTI2020

An Unmanned Aerial System (UAS) for concurrent measurements of solar-induced chlorophyll fluorescence and hyperspectral reflectance toward improving crop monitoring

Unmanned aerial system (UAS)-based remote sensing can provide high spatial- and temporal-resolution crop monitoring for precision management. Existing crop monitoring UAS primarily use conventional techniques such as multi-spectral broadband vegetation indices (VIs) to track canopy structure/biomass. Recently developed lightweight hyperspectral sensors can track crop physiology and performance via complementary signals such as solar-induced chlorophyll fluorescence (SIF) and photochemical reflectance index (PRI) that can be derived from hyperspectral reflectance. However, few existing UAS can acquire high-quality SIF and hyperspectral reflectance. Here, we designed a novel UAS that simultaneously captures far-red SIF and hyperspectral reflectance, using a single bifurcated fiber and motorized arm to measure downwelling and upwelling irradiance. The UAS was tested over (1) a heterogeneous corn field with spatially varying crop yield potential and (2) a set of soybean and corn plots under differing nutrient treatments. Our UAS can maintain stability under low-to-moderate winds (2–3 m/s) with <0.4 m geolocation accuracy, <0.1 m altitude drift, and <1.5° error of the fiber optic from nadir position during scanning. Weekly seasonal campaigns reveal that SIF outperforms conventional VIs such as the normalized difference vegetation index (NDVI) for distinguishing plots with different crop yield potential, especially after canopy closure when NDVI tends to saturate. The UAS can capture the diurnal dynamics of SIF and PRI for both corn and soybean. At the seasonal scale, SIF acquired from the UAS correlates well with that from a fixed SIF tower system ( R 2 = 0.81), which measured the same target albeit with different footprints, demonstrating the capability of UAS in characterizing the seasonal progression of crop activity. In conclusion, our newly developed UAS provides high-quality SIF and hyperspectral reflectance, facilitating mechanistic understanding of the physiological control on photosynthesis dynamics. Our findings also imply that this UAS could enable extension of fixed tower-based systems to monitor multiple targets from diurnal to seasonal scales and improve monitoring of heterogeneous fields.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2026

Autonomous monitoring of algal biomass: Success stories and lessons learned from long-term field deployment

Autonomous, high-frequency monitoring of outdoor algal ponds is needed to quantify biomass productivity and detect culture decline in environments prone to contamination, grazers, and variable operating conditions. We report successes and lessons learned in translating a laboratory spectroradiometric monitoring approach to a multi-year autonomous field deployment at the Arizona Center for Algae Technology and Innovation (AzCATI). The system measures spectrally resolved pond reflectance by ratioing upwelling radiance from each raceway to simultaneous downwelling sky irradiance using fiber-coupled spectrometers. A physics-based reflectance model (ASHARP) is fit to each spectrum pair to estimate optical parameters, including a biomass-proxy coefficient (C a ) which enables near-real-time tracking of biomass accumulation and culture state at 2–5 min intervals. From May 2022 through September 2025 the platform operated continuously while scaling from two to six raceway ponds. Several strains of algae were monitored successfully, including the high productivity Tetraselmis striata and Picochlorum celeri. Transitioning data acquisition from a Windows laptop to a Raspberry Pi improved uptime from 57% (2022) to ~89% (2024–2025) and enabled routine real-time analysis. Further, we converted relative biomass estimates to absolute ash-free dry weight (AFDW) using experimentally-derived calibrations, providing field-relevant biomass predictions with conservative confidence bounds. These results demonstrate the feasibility of long-term, autonomous optical monitoring for well-mixed open-raceway algal cultivation and provide practical guidance for reliable field operation and scaling.

Katinas, Christopher Michael [Sandia National Labo↗
DOE OSTI2024

Effects of boundary hydraulics, dissolved oxygen, and dissolved organic carbon on growth and death dynamics of aerobic microbes in riverbed dune-induced hyporheic zones

Surface and groundwater interact in the hyporheic zone beneath and adjacent to rivers in the presence of a diverse microbial community. Heterotrophic bacteria mediate a range of environmentally important reactions, yet few studies have quantified bacterial growth and death dynamics in the hyporheic zone, and none have systematically analyzed their response to variations in hydraulic or chemical conditions. Here we used MODFLOW and SEAM3D to simulate hydraulics; dissolved oxygen (DO) and dissolved organic carbon (DOC) transport; and aerobic microbial metabolism, growth, and death in hyporheic zones induced by riverbed dunes. We ran simulations both with and without growth/death processes, and varied hydraulic parameters and DO/DOC boundary concentrations. Microbial biomass reached steady state (t = 3 days) in every simulation, at which time there was greater biomass and DOC biodegradation rates in the hyporheic flowcell (300% and 85% higher for the base case, respectively) when accounting for microbial growth dynamics. This occurred as microbial biomass tailored its spatial distribution to the availability of DO and DOC, demonstrating the importance of simulating growth/death processes. Biomass generally increased with hyporheic flow cell area as upwelling groundwater decreased. When varying surface water DO and DOC source concentrations relative to the base case, the greatest effect on biomass occurred when increasing DOC and decreasing DO. We determined minimum DO and DOC steady-state concentrations required for microbial growth, but the minimums were not absolute or related by stoichiometry. Increasing DOC created a smaller area of microbes with higher concentrations relative to the base case. Increasing DO slightly increased the area occupied by microbes while keeping the total biomass nearly constant. Overall, microbial growth and death dynamics depend on DO and DOC availability in the hyporheic zone, which is dependent on DOC/DO boundary concentrations and hyporheic flow paths, and in turn the hydraulic interaction between surface water and groundwater.

54 ENVIRONMENTAL SCIENCES↗