Results for “correlates of protection” — Page 17

NASA NTRS1978

Aerothermodynamic assessment of corrugated panel thermal protection systems

The results of an analysis of wind-tunnel data for the effect of wave shape on local boundary layer flows and heat transfer are used to assess the feasibility of using corrugated panels on a Single-Stage-To-Orbit (SSTO) vehicle. In the flight assessment, available engineering design procedures are used to compute the inviscid and boundary layer flow for a given flight trajectory. The flight vehicle flow field calculations are then used in conjunction with wind-tunnel corrugated panel heating data correlations to examine the heating penalties associated with using corrugated panels at a number of locations on the vehicle. Severe heating penalties, as a result of the corrugations, were found over large areas of the windward surface. Maximum surface temperature penalties ranged from 100K to 500K and very large structural advantages for the corrugations would be required to override the heating penalties. Computed peak corrugation temperatures were within allowable limits of the equivalent smooth wall material for most leeward and side fuselage locations and corrugated panels will be most likely limited to these locations.

Brandon, H. J.↗

DOE OSTI2020

Salmonella Typhoid Toxin PltB Subunit and Its Non-typhoidal Salmonella Ortholog Confer Differential Host Adaptation and Virulence

Typhoidal and non-typhoidal Salmonelleae (NTS) cause typhoid fever and gastroenteritis, respectively, in humans. Salmonella typhoid toxin contributes to typhoid disease progression and chronic infection, but little is known about the role of its NTS ortholog. We found that typhoid toxin and its NTS ortholog induce different clinical presentations. The PltB subunit of each toxin exhibits different glycan-binding preferences that correlate with glycan expression profiles of host cells targeted by each bacterium at the primary infection or intoxication sites. Through co-crystal structures of PltB subunits bound to specific glycan receptor moieties, we show that they induce markedly different glycan-binding preferences and virulence outcomes. Furthermore, immunization with the NTS S. Javiana or its toxin offers cross-reactive protection against lethal-dose typhoid toxin challenge. Cumulatively, these results offer insights into the evolution of host adaptations in Salmonella AB toxins, their cell and tissue tropisms, and the design for improved typhoid vaccines and therapeutics.

59 BASIC BIOLOGICAL SCIENCES↗

DOE OSTI2020

Skyrmion fluctuations at a first-order phase transition boundary

Magnetic skyrmions are topologically protected spin textures with promising prospects for applications in data storage. They can form a lattice state due to competing magnetic interactions and are commonly found in a small region of the temperature—magnetic field phase diagram. Recent work has demonstrated that these magnetic quasi-particles fluctuate at the μeV energy scale. Here, we use a coherent x-ray correlation method at an x-ray free-electron laser to investigate these fluctuations in a magnetic phase coexistence region near a first-order transition boundary where fluctuations are not expected to play a major role. Surprisingly, we find that the relaxation of the intermediate scattering function at this transition differs significantly compared to that deep in the skyrmion lattice phase. The observation of a compressed exponential behavior suggests solid-like dynamics, often associated with jamming. We assign this behavior to disorder and the phase coexistence observed in a narrow field-window near the transition, which can cause fluctuations that lead to glassy behavior.

71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSIC↗

DOE OSTI2026

Light-Induced Charge Order Mode in a Metastable Cuprate Ladder

We report the observation of an emergent charge order mode in the optically excited cuprate ladder Sr 14⁢ Cu 24⁢ O 41 . Near-infrared light in the ladder plane drives a symmetry-protected electronic metastable state together with a partial melting of the equilibrium charge order. Our time-resolved resonant inelastic x-ray scattering measurements at the upper Hubbard band reveal a collective excitation dispersing from the charge order wave vector up to 0.8 eV with a slope on the order of the quasiparticle velocity. These findings reveal a regime where correlated carriers acquire itinerant character at finite momentum, and charge order becomes dynamically fluctuating, offering a platform to explore light-induced pairing instabilities.

75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND↗

NASA NTRS2012

Using an A-10 Aircraft for Airborne measurements of TGFs

Plans are underway to convert an A-10 combat attack aircraft into a research aircraft for thunderstorm research. This aircraft would be configured and instrumented for flights into large, convective thunderstorms. It would have the capabilities of higher altitude performance and protection for thunderstorm conditions that exceed those of aircraft now in use for this research. One area of investigation for this aircraft would be terrestrial gamma ]ray flashes (TGFs), building on the pioneering observations made by the Airborne Detector for Energetic Lightning Emissions (ADELE) project several years ago. A new and important component of the planned investigations are the continuous, detailed correlations of TGFs with the electric fields near the aircraft, as well as detailed measurements of nearby lightning discharges. Together, the x-and gamma-radiation environments, the electric field measurements, and the lightning observations (all measured on microsecond timescales) should provide new insights into this TGF production mechanism. The A -10 aircraft is currently being modified for thunderstorm research. It is anticipated that the initial test flights for this role will begin next year.

Fishman, Gerald J.↗

NASA NTRSDate not supplied

Characterization of Modern Spacecraft Materials under Space-simulated Environment

External spacecraft materials play an important role in satellite protection from the harsh space environment. Research has shown that the physical, chemical, and optical properties of matter change continuously as a result of exposure to solar radiation and aggressive chemical species produced in Earth’s upper atmosphere. Thorough knowledge of the material properties evolution throughout a planned mission lifetime helps to improve the reliability of spacecraft. Moreover, the establishment of correlation factors between true space exposure and accelerated space weather experiments at ground facilities enables accurate prediction of on-orbit material performance based on laboratory-based testing. The presented work aims to evaluate the radiation effects of low Earth orbit (LEO) environment, namely, exposure to the high-energy electrons, atomic oxygen (AO), and vacuum ultraviolet (VUV), of several modern spacecraft materials. The studied materials represent the “flight duplicates” of samples that will be launched as a part of the Materials International Space Station Experiment Flight Facility (MISSE-FF) mission in 2022. MISSE-FF flight sample collection comprises different classes of polymers, including polyimides from the Kapton family, manufactured by E.I du Pont de Nemours and Co., Polyethylene terephthalate (PET) materials, liquid crystal polymers, PI/Polyhedral Oligomeric Silsesquioxanes (POSS), and carbon and glass fiber reinforced polymers. A sequential exposure approach was undertaken to allow monitoring of degradation induced by each environmental component (electrons, AO, and VUV) separately. Surface morphology, optical, and charge transport properties of selected materials were characterized using different techniques, namely, atomic force and scanning electron microscopy, ultraviolet visible (UV/Vis) transmission, reflectance, Bidirectional Reflectance Distribution Function (BRDF), and surface potential decay measurements.

Elena Plis↗

NASA NTRSDate not supplied

Spacecraft Materials Degradation Under Space-Simulated Low Earth Orbit (LEO) Environment

External spacecraft materials play an important role in satellite protection from the harsh space environment. Research has shown that the physical, chemical, and optical properties of matter change continuously as a result of exposure to solar radiation and aggressive chemical species produced in Earth’s upper atmosphere. Thorough knowledge of the material properties evolution throughout a planned mission lifetime helps to improve the reliability of spacecraft. Moreover, the establishment of correlation factors between true space exposure and accelerated space weather experiments at ground facilities enables accurate prediction of on-orbit material performance based on laboratory-based testing. The presented work aims to evaluate the radiation effects of low Earth orbit (LEO) environment, namely, exposure to the high-energy electrons, atomic oxygen (AO), and vacuum ultraviolet (VUV), of several modern spacecraft materials. The studied materials represent the “flight duplicates” of samples that are launched as a part of the 16th Materials International Space Station Experiment Flight Facility (MISSE-FF) mission in 2022.

Elena A Plis↗

NASA NTRSDate not supplied

Ground Testing of the MISSE-16 Materials

External spacecraft materials play an important role in satellite protection from the harsh space environment. Research has shown that the physical, chemical, and optical properties of matter change continuously as a result of exposure to solar radiation and aggressive chemical species produced in Earth’s upper atmosphere. Thorough knowledge of the material properties evolution throughout a planned mission lifetime helps to improve the reliability of spacecraft. Moreover, the establishment of correlation factors between true space exposure and accelerated space weather experiments at ground facilities enables accurate prediction of on-orbit material performance based on laboratory-based testing. The presented work aims to evaluate the radiation effects of low Earth orbit (LEO) environment, namely, exposure to the high-energy electrons and atomic oxygen (AO), of heritage and novel spacecraft materials selection. The studied materials represent the “flight duplicates” of samples that are launched as a part of the 16th Materials International Space Station Experiment Flight Facility (MISSE-FF) mission in 2022.

Elena A. Plis↗

NASA NTRSDate not supplied

Spacecraft Materials Degradation Under Space-Simulated Low Earth Orbit (LEO) Environment

External spacecraft materials play an important role in satellite protection from the harsh space environment. Research has shown that the physical, chemical, and optical properties of matter change continuously as a result of exposure to solar radiation and aggressive chemical species produced in Earth’s upper atmosphere. Thorough knowledge of the material properties evolution throughout a planned mission lifetime helps to improve the reliability of spacecraft. Moreover, the establishment of correlation factors between true space exposure and accelerated space weather experiments at ground facilities enables accurate prediction of on-orbit material performance based on laboratory-based testing. The presented work aims to evaluate the radiation effects of low Earth orbit (LEO) environment, namely, exposure to the high-energy electrons, atomic oxygen (AO), and vacuum ultraviolet (VUV), of several modern spacecraft materials. The studied materials represent the “flight duplicates” of samples that are launched as a part of the 16th Materials International Space Station Experiment Flight Facility (MISSE-FF) mission in 2022.

Elena Plis↗

NASA NTRS2023

Tracking NO2 Pollution Changes Over Texas: Synthesis of In Situ and Satellite Observations

Nitrogen oxides (NO x ) are major air pollutants that play a crucial role in atmospheric chemistry. We compare Ozone Measuring Instrument's (OMI) NO 2 records with the in situ surface measurements from the Air Quality System of the US Environmental Protection Agency and the Texas Commission on Environmental Quality network in the state of Texas with the goal of understanding the correspondence of satellite and in situ surface observations and identifying the potential synergies between the two observing systems. Our analysis of over 40 in situ daily surface site observations, mostly from urban areas, and OMI daily observed data suggests a correlation ( r ) ranging between 0.2 and 0.8. The correlation improves considerably ( r > 0.5) for monthly average data. Weekly variation of surface NO 2 with a Sunday minimum is well captured by OMI tropospheric NO 2 column observations with similar weekend reductions. NO 2 trend in Texas during 2005–2019 is characterized by significant reductions of 20%–36% in highly populated cities and urban centers. However, a significant (up to 80%) increase was observed in oil and gas producing regions of the Permian and Eagle Ford Basins over the same period. In March–April of 2020, like the other US and global cities, Texas experienced up to 60% reduction in NO 2 levels in major cities due to travel restrictions imposed at local and national levels to contain the spread of COVID-19. Though such reduction is temporary, these reductions were significantly larger than those achieved in the past 16 years of OMI record suggesting that technological advancement can curtail NO x emissions.

Nitrogen dioxide (NO2)↗

NASA NTRS2015

Alternate Protocol for Detecting Biological Contamination on Sensitive Hardware

The purpose of this project is to develop a sterile water based rapid bioburden test. Contamination engineers use two tests to assess the level of biological contamination on hardware: the rapid five minute bioburden test, which is a molecular screening for Adenosine triphosphate (ATP), a molecule found in all cells on the hardware, and a slower colony growth test, which is used to give a more accurate representation of the amount of microbes on the hardware. However, the rapid bioburden test has limited application because it leaves a residue that can be detrimental to sensitive hardware. This can cause project delays while waiting for the results from the three day colony growth test. We address this problem by adapting the commercial germicide based ATP system to a sterile water based system. The test works by reacting ATP with D-Luciferin and Luciferase protein to yield light. The light is then detected by a luminometer that outputs a Relative Light Unit (RLU) amount depending on how much ATP is present. To analyze the effectiveness of the new test, we developed a correlation between amounts of ATP and the RLU produced using the germicide based system. From these experiments, we've generated a consistent relationship between the two in the form of a power curve. From there, we developed a correlation curve between the amount of colonies and the RLU they produced. Initial tests of the new protocol have shown that the water based system isn't as sensitive as the germicide based test.

planetary protection↗

DOE OSTI2023

Nuclear Criticality Safety Integral Experiment Covariance Determination

Integral benchmarks for criticality safety and nuclear data validation require expensive uncertainty quantification studies. Commonly, the uncertainty quantification ignores correlations between experiments that share components. Experiments such as the TEX (Thermal/Epithermal eXperiments) campaigns consist of many shared parts, such as fuel, which create a strong correlation in their uncertainties. While these correlations are known to exist, they are often not estimated due to the complexity of such calculations. This paper describes a software package that uses an intuitive method of determining the covariance for each of the experimental components, providing a correlation matrix for each family of parts across the multiple cases examined within a benchmark. The code uses the TEX-HEU campaign as a proof of concept, and we show that the correlations can be calculated with information commonly found in ICSBEP (International Criticality Safety Benchmark Evaluation Project) benchmarks. The estimated covariances are used in χ 2 trending studies to evaluate their impact on nuclear data validation. Without covariances, χ 2 per degree of freedom was calculated as 2.203 and with covariances it was 1.179. The difference shows that omitting covariance information may cause overly pessimistic bias quantifications. The covariance determination code can be easily integrated into current benchmark evaluations as well as reevaluating legacy benchmark uncertainties. Uncertainty correlation calculations should become the baseline for criticality safety integral experiment benchmarks and can now be easily calculated with the described software package.

73 NUCLEAR PHYSICS AND RADIATION PHYSICS↗

DOE OSTI2021

Additive Destabilization of Porous Magnesium Borohydride Framework with Core--Shell Structure

Design of interfaces with thermodynamic and kinetic specificity is of great importance for hydrogen storage from both an applied and fundamental perspective. Here, in order to destabilize the metal hydride and protect the dehydrogenated products from oxidizing, a unique core-shell structure of porous Mg(BH4)2-based framework with a thin layer (no more than 5 nm) of MgCl2 additives on the surface, has been proposed and synthesized via a wet-chemical method. The local structure and electronic state of the present complex system are systematically investigated to understand the correlation between the distribution of additives and dehydrogenation property of Mg(BH4)2. A significant improvement is achieved for hydrogen desorption with chlorides: initial hydrogen release from MgCl2 decorated ..gamma..-phase Mg(BH4)2 particles commences at 100 °C and reaches a maximum of 9.4 wt% at 385 °C. Besides the decreased decomposition temperature, an activation barrier of about 76.4 kJ mol-1 lower than that of Mg(BH4)2 without MgCl2 is obtained. Moreover, MgCl2 decoration can also prevent the whole decomposed system (both Mg- and B- elements) from oxidizing, which is a necessary condition to reversibility.

74 ATOMIC AND MOLECULAR PHYSICS↗

DOE OSTI2024

Labile assembly of a tardigrade protein induces biostasis

Abstract Tardigrades are microscopic animals that survive desiccation by inducing biostasis. To survive drying tardigrades rely on intrinsically disordered CAHS proteins, which also function to prevent perturbations induced by drying in vitro and in heterologous systems. CAHS proteins have been shown to form gels both in vitro and in vivo, which has been speculated to be linked to their protective capacity. However, the sequence features and mechanisms underlying gel formation and the necessity of gelation for protection have not been demonstrated. Here we report a mechanism of fibrillization and gelation for CAHS D similar to that of intermediate filament assembly. We show that in vitro, gelation restricts molecular motion, immobilizing and protecting labile material from the harmful effects of drying. In vivo, we observe that CAHS D forms fibrillar networks during osmotic stress. Fibrillar networking of CAHS D improves survival of osmotically shocked cells. We observe two emergent properties associated with fibrillization; (i) prevention of cell volume change and (ii) reduction of metabolic activity during osmotic shock. We find that there is no significant correlation between maintenance of cell volume and survival, while there is a significant correlation between reduced metabolism and survival. Importantly, CAHS D's fibrillar network formation is reversible and metabolic rates return to control levels after CAHS fibers are resolved. This work provides insights into how tardigrades induce reversible biostasis through the self‐assembly of labile CAHS gels.

59 BASIC BIOLOGICAL SCIENCES↗

DOE OSTI2022

Direct Multimodal Nanoscale Visualization of Early Phosphorus-Based Antiwear Tribofilm Formation

Understanding the mechanism of antiwear (AW) tribofilm formation and how to tune surface chemistry to control functionality is essential for the development of the next generation of oil lubricants. In particular, understanding and optimizing early AW tribofilm formation can increase the energy efficiency of mechanical systems. However, the mechanism for how these films form is not well understood. The majority of prior work has focused on analyzing only end-of-test surfaces long after the film has formed. In this work, we develop an in situ multimodal chemical imaging methodology to directly visualize the early formation of AW films on steel surfaces. We investigate an oil formulation containing a phosphorus-based additive commonly used to protect surfaces from wear and fatigue processes in machine elements, such as gears, bearings, and sliding contacts. Using nanoscale multimodal chemical imaging on combined platforms of atomic force microscopy (AFM) coupled directly with in situ nano-infrared (nano-IR) spectroscopy, and further combined ex situ with time-of-flight secondary ion mass spectrometry (ToF-SIMS), we demonstrate a direct correlation between changes in friction and local surface chemistry. In these experiments, the AFM probe acts as a single asperity contact to generate the tribofilm as well as a tool to analyze it in situ as it is forming. To verify our in situ measurements, we compare these results to the ex situ ToF-SIMS of macroscale block-on-ring tribometer-formed samples. We report the understanding gained here on how AW films form and how film properties can be modified by tuning the chemistry of the additives will facilitate developing transmission fluids to meet increasing demands for vehicle performance and efficiency.

36 MATERIALS SCIENCE↗

NASA NTRS1994

Analysis of the Shuttle Orbiter reinforced carbon-carbon oxidation protection system

Reusable, oxidation-protected reinforced carbon-carbon (RCC) has been successfully flown on all Shuttle Orbiter flights. Thermal testing of the silicon carbide-coated RCC to determine its oxidation characteristics has been performed in convective (plasma Arc-Jet) heating facilities. Surface sealant mass loss was characterized as a function of temperature and pressure. High-temperature testing was performed to develop coating recession correlations for predicting performance at the over-temperature flight conditions associated with abort trajectories. Methods for using these test data to establish multi-mission re-use (i.e., mission life) and single mission limits are presented.

Williams, S. D.↗

NASA NTRS2019

Overexpression of Catalase in Mitochondria Mitigates the Effects of Simulated Microgravity and Social Isolation on Cytokine Expression in Mouse Hippocampus

Space flight missions are becoming longer and more common and evidence points to the physiological toll the missions have on the human body. Aging, sedentary lifestyle, and spaceflight have similar degenerative effects on almost every part of our body; both exposure to the space environment and aging result in cardiovascular deconditioning, bone loss, muscle atrophy, brain changes, and immune response impairment. We hypothesize that exposure to the space environment generates excessive Reactive Oxygen Species (ROS), which results in neuroinflammation and aging-like degenerative symptoms in the brain. We used the hindlimb unloading (HU) model to mimic microgravity with either paired or single housed animals (social isolation). Responses to 30d (30 days) of HU were compared in wildtype or transgenic MCAT (Mitochondrial-targeted CATalase) mice, in which mitochondrial ROS is quenched by over-expression of human catalase. Expression of 4-Hydroxynonenal (4HNE) and Park7 (redox-sensitive chaperone and sensor of oxidative stress) were measured by ELISA (Enzyme-Linked ImmunoSorbent Assay), a protein array quantified from the hippocampal cytokines and 8-hydroxy-2'-deoxyguanosine in serum was measured by ELISA to assess oxidative DNA damage. Preliminary analysis of cage behavior patterns from video collected at the end of the study showed that MCAT HU mice (socially housed) were more active and conducted more exploratory activities compared to NL (Normally Loaded). Our biochemical results showed simulated microgravity and/or social isolation caused changes in levels of cytokines related to immune responses. Two-way ANOVA (Analysis of Variance) revealed significant interaction effects of HU and genotype in expression levels of five cytokines (out of 35) in socially-housed animals. Elevation of these generally pro-inflammatory cytokines by HU in WT (Wild Type) mice was mitigated in MCAT mice, suggesting a role for mitochondrial ROS signaling in inflammatory CNS (Central Nervous System) responses to microgravity. Interestingly, some of these cytokines in the hippocampus displayed strong correlations to the 4HNE levels. We also found substantive cytokine responses to social isolation in the hippocampus; housing and genotype interaction effects were significant (by 2-Factor ANOVA) for 15 cytokines, most of which were mitigated in MCAT mice.Taken together, our results showed that both simulated microgravity and social isolation influenced cytokine levels in the hippocampus and MCAT mice were at least partially protected from these changes. These findings implicate a potentially important role for mitochondrial ROS in CNS responses to the challenges posed by long duration spaceflight.

MCAT↗

DOE OSTI2023

Many-Body Majorana Braiding without an Exponential Hilbert Space

Qubits built out of Majorana zero modes constitute the primary path toward topologically protected quantum computing. Simulating the braiding process of multiple Majorana zero modes corresponds to the quantum dynamics of a superconducting many-body system. It is crucial to study the Majorana dynamics both in the presence of all other quasiparticles and for reasonably large system sizes. We present a method to calculate arbitrary many-body wave functions as well as their expectation values, correlators, and overlaps from time evolved single-particle states of a superconductor, allowing for significantly larger system sizes. We calculate the fidelity, transition probabilities, and joint parities of Majorana pairs to track the quality of the braiding process. Here, we show how the braiding success depends on the speed of the braid. Moreover, we demonstrate the topological CNOT two-qubit gate as an example of two-qubit entanglement. Our Letter opens the path to test and analyze the many theoretical implementations of Majorana qubits. Moreover, this method can be used to study the dynamics of any noninteracting superconductor.

Majorana bound states↗