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At least 307 records · Page 17

Active site plasticity and possible modes of chemical inhibition of the human DNA deaminase APOBEC3B

The single-stranded DNA cytosine deaminase APOBEC3B (A3B) functions in innate immunity against viruses, but it is also strongly implicated in eliciting mutations in cancer genomes. Because of the critical role of A3B in promoting virus and tumor evolution, small molecule inhibitors are desirable. However, there is no reported structure for any of the APOBEC3-family enzymes in complex with a small molecule bound in the active site, which hampers the development of small molecules targeting A3B. Here we report high-resolution structures of an active A3B catalytic domain chimera with loop 7 residues exchanged with those from the corresponding region of APOBEC3G (A3G). The structures reveal novel open conformations lacking the catalytically essential zinc ion, with the highly conserved active site residues extensively rearranged. These inactive conformations are stabilized by 2-pyrimidone or an iodide ion bound in the active site. Molecular dynamics simulations corroborate the remarkable plasticity of the engineered active site and identify key interactions that stabilize the native A3B active site. These data provide insights into A3B active site dynamics and suggest possible modes of its inhibition by small molecules, which would aid in rational design of selective A3B inhibitors for constraining virus and tumor evolution.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

Engagement opportunities in OECD NEA benchmark development

A myriad of opportunities is available to collaborate via international benchmark exercises and experimental data preservation activities. Many such opportunities abound under the auspices of the Nuclear Science Committee of the Organisation for Economic Co-operation and Development Nuclear Energy Agency (NEA). Key projects and activities of relevance to the development of advanced reactors design include the International Criticality Safety Benchmark Evaluation Project (ICSBEP), the International Reactor Physics Experiment Evaluation Project (IRPhEP), the International Assay Data of Spent Nuclear Fuel Database (SFCOMPO), the Shielding Integral Benchmark and Archive Database (SINBAD), and The International Experimental Thermal HYdraulicS Database (TIETHYS), and various cooperative benchmark exercises. Interested participants are encouraged to contact the leadership and secretariat of the various Technical Working Groups and Working Parties to become more engaged. This paper provides a summary of the current benchmark exercises and experimental databases available for international participation.

96 KNOWLEDGE MANAGEMENT AND PRESERVATION↗

Metabolic capability and in situ activity of microorganisms in an oil reservoir

Background: Microorganisms have long been associated with oxic and anoxic degradation of hydrocarbons in oil reservoirs and oil production facilities. While we can readily determine the abundance of microorganisms in the reservoir and study their activity in the laboratory, it has been challenging to resolve what microbes are actively participating in crude oil degradation in situ and to gain insight into what metabolic pathways they deploy. Results: Here, we describe the metabolic potential and in situ activity of microbial communities obtained from the Jiangsu Oil Reservoir (China) by an integrated metagenomics and metatranscriptomics approach. Almost complete genome sequences obtained by differential binning highlight the distinct capability of different community members to degrade hydrocarbons under oxic or anoxic condition. Transcriptomic data delineate active members of the community and give insights that Acinetobacter species completely oxidize alkanes into carbon dioxide with the involvement of oxygen, and Archaeoglobus species mainly ferment alkanes to generate acetate which could be consumed by Methanosaeta species. Furthermore, nutritional requirements based on amino acid and vitamin auxotrophies suggest a complex network of interactions and dependencies among active community members that go beyond classical syntrophic exchanges; this network defines community composition and microbial ecology in oil reservoirs undergoing secondary recovery. Conclusion: Our data expand current knowledge of the metabolic potential and role in hydrocarbon metabolism of individual members of thermophilic microbial communities from an oil reservoir. The study also reveals potential metabolic exchanges based on vitamin and amino acid auxotrophies indicating the presence of complex network of interactions between microbial taxa within the community.

59 BASIC BIOLOGICAL SCIENCES↗

Investigating the Effects of Bars on Star Formation and Nuclear Activity of Galaxies Using DESI Survey Data

We present a statistical analysis of the connections between galactic bars, star formation, and active galactic nucleus (AGN) activity using 33,201 disk galaxies (0.01 < z < 0.05) from Dark Energy Spectroscopic Instrument Data Release 1 (DESI DR1) cross-matched with Galaxy Zoo DESI. Based on morphological classifications, we identify 3508 strongly barred and 8335 weakly barred systems. We find that barred galaxies exhibit a clear bimodal distribution in color–mass space: weak bars are preferentially found in bluer, lower-mass disks, whereas strong bars are more common in massive, redder systems. Strongly barred galaxies are on average more massive and metal rich than unbarred systems. In addition, strong bars enhance central star formation rates (SFRs) in low-mass galaxies but reduce specific SFRs in massive systems, reflecting a dual role where bars initially trigger central star formation but eventually promote quenching by accelerating gas consumption. In terms of nuclear activity, barred galaxies display a higher incidence of AGN activity. The presence of a bar is also associated with an increased fraction of powerful AGN, with the highest proportions found in strongly barred systems. However, the correlations between AGN activity and detailed bar structural parameters are weak, suggesting that the link between bars and nuclear activity is indirect and regulated by multiple factors. Overall, our results support a scenario in which bars facilitate angular momentum transport and gas inflow, thereby driving central star formation and fueling supermassive black hole accretion while operating alongside other processes that shape galaxy evolution.

Liu, Jianfei [Chinese Academy of Sciences (CAS), B↗

ACTIVE REGION MORPHOLOGIES SELECTED FROM NEAR-SIDE HELIOSEISMIC DATA

We estimate the morphology of near-side active regions using near-side helioseismology. Active regions from two data sets, Air Force Data Assimilative Photospheric flux Transport synchronic maps and Global Oscillation Network Group near-side helioseismic maps, were matched and their morphologies compared. Our algorithm recognizes 382 helioseismic active regions between 2002 April 25 and 2005 December 31 and matches them to their corresponding magnetic active regions with 100% success. A magnetic active region occupies 30% of the area of its helioseismic signature. Recovered helioseismic tilt angles are in good agreement with magnetic tilt angles. Approximately 20% of helioseismic active regions can be decomposed into leading and trailing polarity. Leading polarity components show no discernible scaling relationship, but trailing magnetic polarity components occupy approximately 25% of the area of the trailing helioseismic component. A nearside phase-magnetic calibration is in close agreement with a previous far-side helioseismic calibration and provides confidence that these morphological relationships can be used with far-side helioseismic data. Including far-side active region morphology in synchronic maps will have implications for coronal magnetic topology predictions and solar wind forecasts.

79 ASTRONOMY AND ASTROPHYSICS↗

2013 Metropolitan Area Planning Agency External Travel Survey

# 2013 Metropolitan Area Planning Agency External Travel Survey The 2013 Metropolitan Area Planning Agency (MAPA) External Travel Survey was conducted to measure and identify travel patterns into, within, and out of the greater Omaha/Council Bluffs metropolitan area in Nebraska. MAPA sponsored the survey in conjunction with the Federal Highway Administration, the Nebraska Department of Roads, and the Iowa Department of Transportation. ## Data Collection Agency MAPA conducted the survey. ## Methodology The purpose of the survey was to collect information and data needed as input for MAPA’s travel-demand model. The survey employed a combination of nine survey methods and data-collection activities, including Bluetooth technology, intercept surveys, postcard handouts, travel-time studies, vehicle classification counts, and a web-based survey. ## Survey Records Survey records include a total of 729 participants. ## Transportation Data This study provides Bluetooth records and supplementary data for 17,434 passenger trips and 3,123 commercial trips, accounting for 714,218 vehicle miles traveled. Transportation data are available as zipped files. [Download Winzip](http://www.winzip.com/downwz.htm).

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗

2013 Metropolitan Area Planning Agency External Travel Survey

# 2013 Metropolitan Area Planning Agency External Travel Survey The 2013 Metropolitan Area Planning Agency (MAPA) External Travel Survey was conducted to measure and identify travel patterns into, within, and out of the greater Omaha/Council Bluffs metropolitan area in Nebraska. MAPA sponsored the survey in conjunction with the Federal Highway Administration, the Nebraska Department of Roads, and the Iowa Department of Transportation. ## Data Collection Agency MAPA conducted the survey. ## Methodology The purpose of the survey was to collect information and data needed as input for MAPA’s travel-demand model. The survey employed a combination of nine survey methods and data-collection activities, including Bluetooth technology, intercept surveys, postcard handouts, travel-time studies, vehicle classification counts, and a web-based survey. ## Survey Records Survey records include a total of 729 participants. ## Transportation Data This study provides Bluetooth records and supplementary data for 17,434 passenger trips and 3,123 commercial trips, accounting for 714,218 vehicle miles traveled. Transportation data are available as zipped files. [Download Winzip](http://www.winzip.com/downwz.htm).

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗

2013 Metropolitan Area Planning Agency External Travel Survey

# 2013 Metropolitan Area Planning Agency External Travel Survey The 2013 Metropolitan Area Planning Agency (MAPA) External Travel Survey was conducted to measure and identify travel patterns into, within, and out of the greater Omaha/Council Bluffs metropolitan area in Nebraska. MAPA sponsored the survey in conjunction with the Federal Highway Administration, the Nebraska Department of Roads, and the Iowa Department of Transportation. ## Data Collection Agency MAPA conducted the survey. ## Methodology The purpose of the survey was to collect information and data needed as input for MAPA’s travel-demand model. The survey employed a combination of nine survey methods and data-collection activities, including Bluetooth technology, intercept surveys, postcard handouts, travel-time studies, vehicle classification counts, and a web-based survey. ## Survey Records Survey records include a total of 729 participants. ## Transportation Data This study provides Bluetooth records and supplementary data for 17,434 passenger trips and 3,123 commercial trips, accounting for 714,218 vehicle miles traveled. Transportation data are available as zipped files. [Download Winzip](http://www.winzip.com/downwz.htm).

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗

2013 Metropolitan Area Planning Agency External Travel Survey

# 2013 Metropolitan Area Planning Agency External Travel Survey The 2013 Metropolitan Area Planning Agency (MAPA) External Travel Survey was conducted to measure and identify travel patterns into, within, and out of the greater Omaha/Council Bluffs metropolitan area in Nebraska. MAPA sponsored the survey in conjunction with the Federal Highway Administration, the Nebraska Department of Roads, and the Iowa Department of Transportation. ## Data Collection Agency MAPA conducted the survey. ## Methodology The purpose of the survey was to collect information and data needed as input for MAPA’s travel-demand model. The survey employed a combination of nine survey methods and data-collection activities, including Bluetooth technology, intercept surveys, postcard handouts, travel-time studies, vehicle classification counts, and a web-based survey. ## Survey Records Survey records include a total of 729 participants. ## Transportation Data This study provides Bluetooth records and supplementary data for 17,434 passenger trips and 3,123 commercial trips, accounting for 714,218 vehicle miles traveled. Transportation data are available as zipped files. [Download Winzip](http://www.winzip.com/downwz.htm).

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗

2013 Metropolitan Area Planning Agency External Travel Survey

# 2013 Metropolitan Area Planning Agency External Travel Survey The 2013 Metropolitan Area Planning Agency (MAPA) External Travel Survey was conducted to measure and identify travel patterns into, within, and out of the greater Omaha/Council Bluffs metropolitan area in Nebraska. MAPA sponsored the survey in conjunction with the Federal Highway Administration, the Nebraska Department of Roads, and the Iowa Department of Transportation. ## Data Collection Agency MAPA conducted the survey. ## Methodology The purpose of the survey was to collect information and data needed as input for MAPA’s travel-demand model. The survey employed a combination of nine survey methods and data-collection activities, including Bluetooth technology, intercept surveys, postcard handouts, travel-time studies, vehicle classification counts, and a web-based survey. ## Survey Records Survey records include a total of 729 participants. ## Transportation Data This study provides Bluetooth records and supplementary data for 17,434 passenger trips and 3,123 commercial trips, accounting for 714,218 vehicle miles traveled. Transportation data are available as zipped files. [Download Winzip](http://www.winzip.com/downwz.htm).

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗

2013 Metropolitan Area Planning Agency External Travel Survey

# 2013 Metropolitan Area Planning Agency External Travel Survey The 2013 Metropolitan Area Planning Agency (MAPA) External Travel Survey was conducted to measure and identify travel patterns into, within, and out of the greater Omaha/Council Bluffs metropolitan area in Nebraska. MAPA sponsored the survey in conjunction with the Federal Highway Administration, the Nebraska Department of Roads, and the Iowa Department of Transportation. ## Data Collection Agency MAPA conducted the survey. ## Methodology The purpose of the survey was to collect information and data needed as input for MAPA’s travel-demand model. The survey employed a combination of nine survey methods and data-collection activities, including Bluetooth technology, intercept surveys, postcard handouts, travel-time studies, vehicle classification counts, and a web-based survey. ## Survey Records Survey records include a total of 729 participants. ## Transportation Data This study provides Bluetooth records and supplementary data for 17,434 passenger trips and 3,123 commercial trips, accounting for 714,218 vehicle miles traveled. Transportation data are available as zipped files. [Download Winzip](http://www.winzip.com/downwz.htm).

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗

2013 Metropolitan Area Planning Agency External Travel Survey

# 2013 Metropolitan Area Planning Agency External Travel Survey The 2013 Metropolitan Area Planning Agency (MAPA) External Travel Survey was conducted to measure and identify travel patterns into, within, and out of the greater Omaha/Council Bluffs metropolitan area in Nebraska. MAPA sponsored the survey in conjunction with the Federal Highway Administration, the Nebraska Department of Roads, and the Iowa Department of Transportation. ## Data Collection Agency MAPA conducted the survey. ## Methodology The purpose of the survey was to collect information and data needed as input for MAPA’s travel-demand model. The survey employed a combination of nine survey methods and data-collection activities, including Bluetooth technology, intercept surveys, postcard handouts, travel-time studies, vehicle classification counts, and a web-based survey. ## Survey Records Survey records include a total of 729 participants. ## Transportation Data This study provides Bluetooth records and supplementary data for 17,434 passenger trips and 3,123 commercial trips, accounting for 714,218 vehicle miles traveled. Transportation data are available as zipped files. [Download Winzip](http://www.winzip.com/downwz.htm).

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗

2013 Metropolitan Area Planning Agency External Travel Survey

# 2013 Metropolitan Area Planning Agency External Travel Survey The 2013 Metropolitan Area Planning Agency (MAPA) External Travel Survey was conducted to measure and identify travel patterns into, within, and out of the greater Omaha/Council Bluffs metropolitan area in Nebraska. MAPA sponsored the survey in conjunction with the Federal Highway Administration, the Nebraska Department of Roads, and the Iowa Department of Transportation. ## Data Collection Agency MAPA conducted the survey. ## Methodology The purpose of the survey was to collect information and data needed as input for MAPA’s travel-demand model. The survey employed a combination of nine survey methods and data-collection activities, including Bluetooth technology, intercept surveys, postcard handouts, travel-time studies, vehicle classification counts, and a web-based survey. ## Survey Records Survey records include a total of 729 participants. ## Transportation Data This study provides Bluetooth records and supplementary data for 17,434 passenger trips and 3,123 commercial trips, accounting for 714,218 vehicle miles traveled. Transportation data are available as zipped files. [Download Winzip](http://www.winzip.com/downwz.htm).

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗

2013 Metropolitan Area Planning Agency External Travel Survey

# 2013 Metropolitan Area Planning Agency External Travel Survey The 2013 Metropolitan Area Planning Agency (MAPA) External Travel Survey was conducted to measure and identify travel patterns into, within, and out of the greater Omaha/Council Bluffs metropolitan area in Nebraska. MAPA sponsored the survey in conjunction with the Federal Highway Administration, the Nebraska Department of Roads, and the Iowa Department of Transportation. ## Data Collection Agency MAPA conducted the survey. ## Methodology The purpose of the survey was to collect information and data needed as input for MAPA’s travel-demand model. The survey employed a combination of nine survey methods and data-collection activities, including Bluetooth technology, intercept surveys, postcard handouts, travel-time studies, vehicle classification counts, and a web-based survey. ## Survey Records Survey records include a total of 729 participants. ## Transportation Data This study provides Bluetooth records and supplementary data for 17,434 passenger trips and 3,123 commercial trips, accounting for 714,218 vehicle miles traveled. Transportation data are available as zipped files. [Download Winzip](http://www.winzip.com/downwz.htm).

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗

2013 Metropolitan Area Planning Agency External Travel Survey

# 2013 Metropolitan Area Planning Agency External Travel Survey The 2013 Metropolitan Area Planning Agency (MAPA) External Travel Survey was conducted to measure and identify travel patterns into, within, and out of the greater Omaha/Council Bluffs metropolitan area in Nebraska. MAPA sponsored the survey in conjunction with the Federal Highway Administration, the Nebraska Department of Roads, and the Iowa Department of Transportation. ## Data Collection Agency MAPA conducted the survey. ## Methodology The purpose of the survey was to collect information and data needed as input for MAPA’s travel-demand model. The survey employed a combination of nine survey methods and data-collection activities, including Bluetooth technology, intercept surveys, postcard handouts, travel-time studies, vehicle classification counts, and a web-based survey. ## Survey Records Survey records include a total of 729 participants. ## Transportation Data This study provides Bluetooth records and supplementary data for 17,434 passenger trips and 3,123 commercial trips, accounting for 714,218 vehicle miles traveled. Transportation data are available as zipped files. [Download Winzip](http://www.winzip.com/downwz.htm).

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗

2013 Metropolitan Area Planning Agency External Travel Survey

# 2013 Metropolitan Area Planning Agency External Travel Survey The 2013 Metropolitan Area Planning Agency (MAPA) External Travel Survey was conducted to measure and identify travel patterns into, within, and out of the greater Omaha/Council Bluffs metropolitan area in Nebraska. MAPA sponsored the survey in conjunction with the Federal Highway Administration, the Nebraska Department of Roads, and the Iowa Department of Transportation. ## Data Collection Agency MAPA conducted the survey. ## Methodology The purpose of the survey was to collect information and data needed as input for MAPA’s travel-demand model. The survey employed a combination of nine survey methods and data-collection activities, including Bluetooth technology, intercept surveys, postcard handouts, travel-time studies, vehicle classification counts, and a web-based survey. ## Survey Records Survey records include a total of 729 participants. ## Transportation Data This study provides Bluetooth records and supplementary data for 17,434 passenger trips and 3,123 commercial trips, accounting for 714,218 vehicle miles traveled. Transportation data are available as zipped files. [Download Winzip](http://www.winzip.com/downwz.htm).

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗

Truck Technology Efficiency Assessment (TTEA) Project (Final Report)

Medium- and heavy-duty trucks and buses are responsible for over 28% of the energy used and emissions generated in highway transportation, and class 8 tractor-trailers operating in long-haul and regional cargo transport are responsible for about 75% of all fuel consumed by commercial trucks. The vehicle miles traveled (VMT) for trucks is expected to increase at a rate significantly outpacing passenger VMT growth, which will result in a steady rise in the percentage of energy consumption (and emissions) attributable to trucks over the coming decades. These facts have sparked significant recent interest in truck fuel efficiency in the transportation community. Although fuel economy regulations in the United States have historically focused on passenger cars, recent legislation requiring new standards for fuel economy in medium- and heavy-duty trucks aims to increase the efficiency of trucks as well. The development of regulations for truck fuel efficiency is quite challenging, however, since vehicle usage and configurations vary substantially among the very diverse set of trucking applications. Fuel economy is very strongly linked to the particular drive cycles followed by a given truck, as are the gains in efficiency that can realized by implementing new technologies. As demonstration of this fact, a technology that provides significant fuel efficiency gains for one trucking application may yield little improvement or could even be detrimental to fuel economy in a different trucking application. It is therefore critical that the usage of each application be well understood and carefully evaluated to select the set of technologies that can provide the greatest benefits for each application. Although it is well known that drive cycle data representative of a vehicle or fleet’s usage is crucial for an accurate evaluation of fuel economy benefits or to identify an optimum set of technologies to reduce fuel consumption, detailed drive cycle data of trucks are not readily available for many applications. The Oak Ridge National Laboratory (ORNL) has collected a rather extensive set of truck duty cycle data in an effort to characterize the usage of several trucking applications. During the first phase of ORNL’s duty cycle data collection activities, drive cycle measurements were made from six tractor-trailers during normal operations in a regional commercial shipping fleet, operating primarily in the southeastern U.S., for a period of a full year. This data, contained in the Heavy Truck Duty Cycle (HTDC) project database, was analyzed in detail for the current research effort in the Truck Technology Efficiency Assessment project. Based on the high fidelity HTDC duty cycle data, the results of this study are therefore highly representative of tractor-trailer operations for this type of regional, freeway-dominant trucking application.

LaClair, Tim J.↗

Application of activity pencil beam algorithm using measured distribution data of positron emitter nuclei for therapeutic SOBP proton beam

Purpose: Recently, much research on imaging the clinical proton-irradiated volume using positron emitter nuclei based on target nuclear fragment reaction has been carried out. The purpose of this study is to develop an activity pencil beam (APB) algorithm for a simulation system for proton-activated positron-emitting imaging in clinical proton therapy using spread-out Bragg peak (SOBP) beams.Methods: The target nuclei of activity distribution calculations are {sup 12}C nuclei, {sup 16}O nuclei, and {sup 40}Ca nuclei, which are the main elements in a human body. Depth activity distributions with SOBP beam irradiations were obtained from the material information of ridge filter (RF) and depth activity distributions of compounds of the three target nuclei measured by BOLPs-RGp (beam ON-LINE PET system mounted on a rotating gantry port) with mono-energetic Bragg peak (MONO) beam irradiations. The calculated data of depth activity distributions with SOBP beam irradiations were sorted in terms of kind of nucleus, energy of proton beam, SOBP width, and thickness of fine degrader (FD), which were verified. The calculated depth activity distributions with SOBP beam irradiations were compared with the measured ones. APB kernels were made from the calculated depth activity distributions with SOBP beam irradiations to construct a simulation system using the APB algorithm for SOBP beams.Results: The depth activity distributions were prepared using the material information of RF and the measured depth activity distributions with MONO beam irradiations for clinical therapy using SOBP beams. With the SOBP width widening, the distal fall-offs of depth activity distributions and the difference from the depth dose distributions were large. The shapes of the calculated depth activity distributions nearly agreed with those of the measured ones upon comparison between the two. The APB kernels of SOBP beams were prepared by making use of the data on depth activity distributions with SOBP beam irradiations that were made from the depth activity distributions with MONO beam irradiations and sorted in terms of energy, SOBP width, and thickness of FD. The data on APB kernels of SOBP beams were determined as installment data for the simulation system using the APB algorithm for SOBP beam irradiations.Conclusions: A method of obtaining the depth activity distributions and the APB algorithm for clinical use of SOBP beams have been developed. It is suggested that the simulation system for imaging the clinical irradiated volume with the APB algorithm can be used in clinical proton therapy using SOBP beams by preparing and investigating the data on APB kernels of SOBP beams.

62 RADIOLOGY AND NUCLEAR MEDICINE↗