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At least 307 records · Page 17

Visualization of local phosphatidylcholine synthesis within hippocampal neurons using a compartmentalized culture system and imaging mass spectrometry

Highlights: • A compartmentalized neuron culture system was combined with imaging mass spectrometry. • Phosphatidylcholine (PC) species within rat hippocampal neurons were visualized. • Differential synthetic activity of a PC species within neurons was observed. • Neurites synthesized a PC species using local extracellular free fatty acids in situ. Neurons extend neurites with an increased synthesis of phosphatidylcholine (PC) that is not only a membrane component but also a functional regulator with specific fatty acid composition. To analyze the local synthesis of the PC molecular species within neurons, we combined a compartmentalized culture system with matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS). We observed that a newly synthesized PC, which contains exogenously administered palmitic acid-d{sub 3}, is accumulated at the cell bodies and the tips of the distal neurites. The local accumulation within distal neurites is formed by distinct metabolic activity from cell bodies, suggesting that the local extracellular composition of free fatty acid can be a key to regulate specific functions of each PC molecular species. We expect our simple method to be a starting point for more sophisticated in vitro analytical methods for unveiling detailed lipid metabolisms within neurons.

60 APPLIED LIFE SCIENCES↗

An approach for visualizing the spatial metabolome of an entire plant root system inspired by the Swiss-rolling technique

The spatial configuration and morphology of roots are commonly monitored for a better understanding of plant health and development. However, this approach provides minimal details about the biochemistry regulating the observable traits. Therefore, the ability to metabolically map the entire root structure would be of major value. Here, we developed a sample preparation approach that enables imaging of the entire root within a restricted space (width of microscope slide), which was influenced by the Swiss-rolling technique. By using this technique, we were able to image and confidently identify molecules along the entire root structure from rolled-root tissue sections using multiple spatially resolved mass spectrometry approaches.

59 BASIC BIOLOGICAL SCIENCES↗

Vehicle detection based on visual attention mechanism and adaboost cascade classifier in intelligent transportation systems

Robust and efficient vehicle detection is an essential task in intelligent transportation systems (ITS). Unfortunately, due to a great diversity of vehicle profiles and outdoor illumination conditions, it is a challenge to detect vehicles effectively. This paper proposes a method for high-performance vehicle detection based on visual attention mechanism and AdaBoost cascade classifier. Our method constructs the structural Haar features and extracts the features of samples using structural Haar features and trains an AdaBoost cascade classifier. Then we use the visual attention mechanism to extract the target candidate region. At last, we generate detecting sub-windows in the candidate region and discriminate them with the cascade classifier to realize vehicle detection. We compare the performance of this method against two variants, one using MB–LBP features and another using Haar features. The experimental results demonstrate satisfactory performance for the proposed method in term of training speed, detecting speed and detecting accuracy.

71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSIC↗

A graphical user interface platform to support power electronic converters and integrated systems

Power Electronic Systems have grown in size and complexity over the past years. Therefore, users require interfaces to properly configure, develop, and deploy these systems. This work presents a Graphical User Interface that dynamically adapts to the system and allows the user to control and monitor its operation. This GUI uses networked communication and open-source Python libraries to allow the user to interact with the system at its different hierarchical layers, as well as visualize the interconnection of the full system. This is demonstrated by showing the user interface applied to a megawatt class charging station that consists of various energy resources.

Dean, Ben↗

Systems and methods of generating a plurality of data visualizations in a data visualization region

An exemplary method of generating a plurality of data visualizations comprises displaying a graphical user interface that includes a schema information region and a data visualization region. The method further includes receiving user actions to associate first, second, and third fields included in the schema information region with a plurality of shelves of the data visualization region. The method further includes generating a plurality of data visualizations in the data visualization region in accordance with the received user actions, each data visualization includes a plurality of data marks corresponding to a respective retrieved tuple of data having the first, second, and third fields. Moreover, each of the data marks has an x-position and y-position defined according to data for the first and second fields, respectively. In addition, each of the data visualizations corresponds to a respective unique value of the third field, and is displayed in a respective pane.

97 MATHEMATICS AND COMPUTING↗

Systems and methods of generating data marks in data visualizations

An example method of displaying a data visualization includes displaying a graphical user interface that includes three distinct regions, and receiving user actions to associate two different fields with two different shelves. The method also includes generating, in accordance with the received user actions, data marks to be displayed in a data visualization, each data mark corresponding to a respective retrieved tuple of data from a multidimensional database, where (i) each data mark has an x-position defined according to data for a first field in the respective tuple and (ii) each data mark has a y-position defined according to data for a second field in the respective tuple. The method also includes displaying a data visualization that includes the generated data marks.

Stolte, Chris↗

Systems and methods of generating data marks in data visualizations

An example method of displaying a data visualization includes displaying a plurality of selectable fields and receiving user selections of two different fields from the plurality of selectable fields. The method also includes generating, in accordance with the received user selections, data marks to be displayed in a data visualization, each data mark corresponding to a respective retrieved tuple of data from a multidimensional database, where (i) each data mark has an x-position defined according to data for a first field in the respective tuple and (ii) each data mark has a y-position defined according to data for a second field in the respective tuple. The method also includes displaying the data visualization that includes the generated data marks.

97 MATHEMATICS AND COMPUTING↗

Evaluation of integrated daylighting and electric lighting design projects: Lessons learned from international case studies

This article presents and discusses the lessons learned from the monitoring of 25 integrated daylighting and electric lighting international case study projects. The case studies consist of real occupied buildings that have been monitored as part of the International Energy Agency (IEA) SHC Task 61/EBC Annex 77 Programme. The general goal of the case studies was to balance lighting energy use with occupants’ visual and non-visual requirements. This was achieved using innovative solutions for daylighting and electric lighting with advanced controls, but also implementing simple and out-of-the-box strategies. The findings suggest that energy demands for lighting can significantly be reduced by combining sensible daylight provision, efficient lighting sources, and advances in controls. Yet, the effective achievement of project goals requires adequate monitoring, fine-tuning, and verification. The findings also suggest that the adoption of “integrative” lighting – that is, lighting systems that address both visual and non-visual responses – is getting increasingly popular. Catering to non-visual requirements will likely drive further innovation in lighting technology. Currently, there is limited investment available for developing daylighting systems for integrative lighting, and the current related electric strategies often come at the risk of energy rebound effects. Overall, providing daylighting and understanding user requirements are fundamental steps towards achieving quality projects, with potential benefits beyond saving energy.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗

3D Modeling of Safety-Related Upgrade Pilot Project

Constellation Energy and the United States Department of Energy (DOE) have established a public/private partnership to implement a pilot digital upgrade to replace legacy analog, safety-related reactor protection and emergency safety feature actuation systems (RPS/ESFAS) with modern digital systems. This effort is occurring at Constellation’s Limerick Generating Station. This project is being performed in accordance with industry processes that have been adapted to better support digital upgrades. These processes include IP-ENG-001, Standard Design Process, NISP-EN-04, Standard Digital Engineering Process and Electric Power Research Institute Report 3002011816, Digital Engineering Guide. The Light Water Reactor Sustainability (LWRS) Program at the Idaho National Laboratory (INL) has been supporting this effort. Latest INL HFE efforts in support of this project have focused on definition and implementation of the Human Factors Engineering (HFE) Program in support of Constellation design and related licensing efforts. This paper will present the development of the HFE 3D modeling execution of the Main Control Room safety upgrades. New equipment and modifications are planned for upgrading the new safety systems. 3D modeling allows for visualization of the new equipment and modifications to be shared with engineering and design teams for evaluation and review. Anthropometric considerations such as sight lines and functional reach can be evaluated directly using the 3D model. This effort has helped to provide visualizations for additional review with operations personnel and project stakeholders.

3D Modeling↗

ADMS Evaluation Platform

Deploying an ADMS or looking to optimize its value? NREL offers a low-cost, low-risk evaluation platform for assessing ADMS performance. The National Renewable Energy Laboratory (NREL) has developed a vendor-neutral advanced distribution management system (ADMS) evaluation platform and is expanding its capabilities. The platform uses actual grid-scale hardware, large-scale distribution system models, and advanced visualization to simulate realworld conditions for the most accurate ADMS evaluation and experimentation.

24 POWER TRANSMISSION AND DISTRIBUTION↗

Theoretical study of chromophores for biological sensing: Understanding the mechanism of rhodol based multi-chromophoric systems

Development of two-photon fluorescent probes can aid in visualizing the cellular environment. Multi-chromophore systems display complex manifolds of electronic transitions, enabling their use for optical sensing applications. Time-Dependent Density Functional Theory (TDDFT) methods allow for accurate predictions of the optical properties. These properties are related to the electronic transitions in the molecules, which include two-photon absorption cross-sections. We use TDDFT to understand the mechanism of aza-crown based fluorescent probes for metals sensing applications. Here our findings suggest changes in local excitation in the rhodol chromophore between unbound form and when bound to the metal analyte. These changes are caused by a charge transfer from the aza-crown group and pyrazol units toward the rhodol unit. Understanding this mechanism leads to an optimized design with higher two-photon excited fluorescence to be used in medical applications.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

Visualization Within the Department of Energy: NREL IEEE VIS Application Spotlight

This presentation highlights the role of advanced visualization techniques at the National Renewable Energy Laboratory (NREL) in supporting cutting-edge research across diverse energy domains. From immersive analytics and uncertainty visualization to high-resolution and real-time data analysis, NREL's visualization capabilities enable scientists to explore complex datasets more effectively. These tools are critical for advancing research in materials science, renewable energy technologies, biofuels, electric vehicle infrastructure, energy efficiency - from industrial processes to entire communities - and then bringing these innovations to practice through energy systems integration. NREL's visualization tools drive innovation across renewable energy and grid modernization efforts by providing deeper insights and improving decision-making.

grid modernization↗

The Strategic Value of Human Factors Engineering in Control Room Modernization

Control room modernization is one of the most challenging and complex upgrade projects that a nuclear power plant can undertake. It can have almost as big an impact on operations as, for example, turbine replacement. The challenges of migrating an analog control system to a distributed control system are already well known and a number of nuclear utilities have embarked upon various levels of effort to upgrade some of the systems in the control room. When planning for control room upgrades, plants have to deal with a multitude of engineering, operational, and regulatory impacts. This will inevitably include several human factors considerations, such as workstation ergonomics, viewing angles, lighting, seating, new interaction modalities, new communication requirements, and new concepts of operation. In helping nuclear power utilities to deal with these challenges, the United States Department of Energy researchers located at Idaho National Laboratory (INL) have developed research-based human factors design and evaluation methods to be used in the development of end-state concepts for modernized control rooms and to manage the various phases of the upgrade life cycle. The methodology includes interactive sessions with operators in INL’s Human System Simulation Laboratory, three-dimensional modeling to visualize control board changes and operator-system interaction, and development of human-system prototypes to evaluate various aspects of proposed modifications. This methodology has been applied at a number of U.S. nuclear power plants where modernization projects are underway, including Exelon’s Braidwood and Byron plants, and Arizona Public Service's Palo Verde plant. It was demonstrated that including this methodology in the plant’s engineering process helps to ensure an integrated and cohesive outcome that is consistent with human factors engineering principles and provide substantial improvement in operator performance.

99 GENERAL AND MISCELLANEOUS↗

Exploring the Use of Large Immersive Display Systems in the Nuclear Industry

Large immersive display systems play a critical role in the nuclear industry by enabling advanced training, design reviews, scientific visualization, and safety simulations. These systems, such as CAVE and powerwalls, allow engineers and operators to interact with virtual nuclear environments in real-time, providing a deeper understanding of complex systems. Their ability to simulate real-world scenarios for testing and optimization in a safe, controlled environment ensures that operators and engineers can refine process, enhance safety protocols, and troubleshoot complex challenges. This article outlines key lessons learned from deploying these systems, practical insights into their applications, and considerations for future improvements, aiming to guide their broader adoption and effective use in the nuclear industry.

99 - GENERAL AND MISCELLANEOUS↗

Water Cleaning System for the Exhaust Pipe of HLW Glass Melter (LFCM) - 17180

Vitrification technology has been generally applied to stabilize High-Level Radioactive Waste (HLW) generated from spent fuel reprocessing. In Japan, Joule Heated Liquid Fed Ceramic Melter (LFCM) system is selected for HLW vitrification and the recent active test has been successfully demonstrated its stable operation. The higher reliability of off-gas treatment system (OGTS) which consists of wet scrubber, absorbers and filters connected to the LFCM is also important to ensure long term system operation. The OGTS removes radioactive gases, mists and dust generated by LFCM and maintains radioactive containments by keeping negative pressure inside. In the past active and inactive tests, it was observed that differential pressure in the exhaust pipe attached to LFCM gradually increased during continuous operation and some deposits were observed inside the off-gas pipe. So the cleaning operation needs to be improved and more frequent in order to avoid the exhaust pipe clogging. Therefore we investigated several cleaning systems and operational methods for the exhaust pipe of LFCM. As a result, we have successfully developed the modified water cleaning system. In this presentation we will introduce our water cleaning system. In addition, we will present the result of inactive mock-up tests for the visualization of this water cleaning system. (authors)

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗

Effectiveness of supercritical-CO 2 and N 2 huff-and-puff methods of enhanced oil recovery in shale fracture networks using microfluidic experiments

Current oil recovery methods in hydraulically fractured shale reservoirs have a low recovery efficiency of about 10%. The objective of this work is to investigate the effectiveness of nitrogen and supercritical carbon dioxide in the huff-and-puff method for enhanced oil recovery as a means to re-energize reservoirs and improve recovery rates. Here, we conduct direct visualization experiments with a microfluidic system to reveal the mechanisms and to quantify the recovery rates of oil from fracture networks. We compared the effectiveness of water, nitrogen and supercritical carbon dioxide at reservoir conditions in a process mimicking the huff-and-puff method in both dead-end and connected fracture systems. The microfluidic chips were made of glass and placed in a confining pressure system pressurized to 10 MPa, 50 °C. The system was allowed to equilibrate, and then depressurized to simulate huff-and-puff oil recovery. Fluorescence microscope images were continuously taken to visualize and calculate residual oil saturation as a function of pressure drawdown. As the system was depressurized from 10 MPa, gas exsolution from the oil liquid phase, including bubble nucleation, growth, and coalescence, appeared to be the main driver for mobilizing oil from the fracture networks. Injection of supercritical CO 2 resulted in the highest recovery rate with an average end-point recovery of about 90% in the connected fracture network and 60% in the dead-end fracture network. N 2 has lower solubility in oil and hence showed a lower recovery rate of 40% in the connected fracture network and 25% in the dead-end fracture network. Injection of water had no effect on oil mobilization since water is insoluble, immiscible and incompressible. The main mechanism of enhanced recovery was gas exsolution from the liquid phase as pressure was decreased below the bubble point pressure. Because the gas was distributed throughout the oil phase, bubble nucleation, growth, coalescence, and elongation occurred throughout the fracture network. Expansion of the gas forced oil out of the network through piston displacement in continuous oil areas and film flow in the dispersed oil areas. Bubbles began as spheres and grew until they touched the fracture walls where they elongated along the fracture length. The bubble growth rate depended on local mass transfer from liquid to gas phase and gas volume expansion due to pressure drop. The efficiency of the huff-and-puff process is dependent on the solubility and miscibility of the injection fluid with oil. High gas solubility allows for more bubble nucleation, growth and expansion during the depressurization cycle.

58 GEOSCIENCES↗