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At least 307 records · Page 17

System to Perform Radio Frequency Interferometry Using Optical Fiber Sensing Signal Processing Techniques

Aspects of the present disclosure involve a system and method for performing radio frequency interferometry using optical fiber sensing. Optical fiber sensing is performed as a reference signal is defined and compared, in the optical domain, to incoming signals to obtain interference fringe patterns that can be used to decode phase shift offsets with respect to the designated reference signal. The phase shift offsets can be determined by first optically modulating the reference and incoming signals using a laser source as the carrier. In the optical domain, the reference and incoming signals are combined using an optical coupler and then converted back to the electrical domain for processing.

Ott, Melanie N.↗

Cassini Spacecraft Strategy for Recovering from a Complete Loss-Of-Signal Condition

Unlike other faults where the space craft’s automated Fault Protection (FP) routines can be relied upon to provide a carrier signal and telemetered data to the ground-based Spacecraft Operations Flight Support (SOFS) team to facilitate vehicle acquisition, diagnosis, and fault recovery, a complete Loss-of-Spacecraft-Signal (LOS) condition will yield no information at all to help re-acquire the vehicle and resolve the fault. For any FP response activation, the spacecraft’s downlink signal must be acquired via the Deep Space Network (DSN) on the expected (post-fault) Radio Frequency Subsystem (RFS) device configuration commanded by the FP; data that will not be attainable in the case of a complete LOS condition since there is no signal available for the ground station to lock onto. In the case of the Cassini Mission-to-Saturn spacecraft, numerous fault possibilities exist that can cause a LOS condition. Activations of the onboard RFS-related FP can reconfigure the post-fault RFS states, impeding the ground team’s ability to acquire the spacecraft’s downlink signal

Morgan, Paula S.↗

Robust Positioning in the Presence of Multipath and NLOS GNSS Signals

GNSS signals can be blocked and reflected by nearby objects, such as buildings, walls, and vehicles. They can also be reflected by the ground and by water. These effects are the dominant source of GNSS positioning errors in dense urban environments, though they can have an impact almost anywhere. Non- line-of-sight (NLOS) reception occurs when the direct path from the transmitter to the receiver is blocked and signals are received only via a reflected path. Multipath interference occurs, as the name suggests, when a signal is received via multiple paths. This can be via the direct path and one or more reflected paths, or it can be via multiple reflected paths. As their error characteristics are different, NLOS and multipath interference typically require different mitigation techniques, though some techniques are applicable to both. Antenna design and advanced receiver signal processing techniques can substantially reduce multipath errors. Unless an antenna array is used, NLOS reception has to be detected using the receiver's ranging and carrier-power-to-noise-density ratio (C/N0) measurements and mitigated within the positioning algorithm. Some NLOS mitigation techniques can also be used to combat severe multipath interference. Multipath interference, but not NLOS reception, can also be mitigated by comparing or combining code and carrier measurements, comparing ranging and C/N0 measurements from signals on different frequencies, and analyzing the time evolution of the ranging and C/N0 measurements.

McGraw, Gary A.↗

Volume Raycasting of GNSS Signals through Ground Structure Lidar for UAV Navigational Guidance and Safety Estimation

Autonomous UAS navigation at low altitudes is often hindered by degradation of GNSS position estimates. The line of sight from the UAS to orbital satellites may be intersected by foliage (which attenuates the received signal) and by buildings (which block the signal). Since the geometric ray from the presumed UAS position to each GNSS satellite orbital location is predictable, if a 3D survey of ground structures is available, the degree of blockage of each GNSS signal can be estimated. In this study we show raycasting from a UAS location to GNSS satellites at two flight locations: one with overlying structures and bordered by tall trees, and another in an arboreal canyon bordered by tall trees. We confirm the intermittent blockage of satellites in the first location sufficient to lose GNSS position fix. We demonstrate low-altitude GNSS fidelity forecasting via the raycasting method at the second location that can be used to plan navigable flight locations and altitudes. Finally, we match the GNSS signal strength with raycast-derived foliage obstruction depth at hundreds of observation times from 55 recordings collected over 14 days from November 2018 to February 2021 at the second location. This matching confirms that signal attenuation varies with the depth of foliage blockage along a saturating exponential curve, as found in prior continuous-wave RF studies. The exponent and saturation value are species dependent and therefore vary from site to site; once determined empirically, they can be used to characterize foliage along a particular flight path, and refine GNSS fidelity forecasts of flights along that path. The techniques described in this study show the feasibility of a survey method to construct low-altitude navigation safety maps and forecasts.

Navigation↗

Precision of Spacecraft Doppler Tracking at Low Signal-to-Noise Ratios

The signal-to-noise ratio received at Earth is typically larger than 10 dB-Hz for radio tracking of a spacecraft in deep space, allowing a phase-locked loop to execute reliable carrier tracking. Recently, missions have been proposed to utilize low-gain antennas for Doppler tracking where the signal-to-noise ratio may be at the single-digit (dB-Hz) level. In this work, we discuss spacecraft Doppler tracking at these low signal-to-noise ratios through an analysis of thermal noise on the radio link, results from ground testing with the Deep Space Network, and demonstrations with an active spacecraft. We show that by utilizing an open-loop receiver to capture the carrier signal and by applying post-processing techniques, radio data with the signal-to-noise ratio as low as 4 dB-Hz can be used to derive Doppler data with precision sufficient for tracking a spacecraft in deep space.

D. R. Buccino↗

Transfer function models for using empirical and physics-based simulation signal response data

In many situations, real or induced flaws such as tight cracks with known morphology cannot be manufactured in part geometry specimens or in real parts. Typically, surface fatigue cracks are manufactured in simple geometry specimens such as flat plates, dog-bone shaped flat or cylindrical specimens. If a nondestructive evaluation (NDE)technique is required to provide a reliably detectable flaw size, denoted asa90/95, for induced flaws in a part, then a direct method for qualifying the NDE procedure is to use appropriate induced flaw specimens and perform NDE procedure demonstration on the specimens. Probability of detection (POD)analysis of the empirical data may provide estimation of a90/95. This approach is described as direct POD demonstration testing, which may follow guidelines of MIL-HDBK-1823. This paper considers a case, where embedded tight cracklike induced flaws are to be detected reliably using a signal response based NDE procedure. Here, it is assumed that it is not practical to make surface or embedded induced flaw specimens in part geometry or configuration. Therefore, a direct POD demonstration testing cannot be undertaken. It is also assumed that simulation of signal response is possible for both surface and embedded induced flaws in part geometry specimens using a physics-based model. The proposed approach for NDE procedure qualification uses artificial flaws in simple geometry and part geometry specimens, and induced flaws in the same type of simple geometry specimens. Signal response data is taken on all sets of artificial and induced flaws in simple geometry and part geometry specimens. Moreover, simulated signal response data is generated for surface and embedded flaws. Thus, a case of five signal response versus flaw size datasets is considered. Three of the datasets are empirical and two datasets are physics model-based simulation datasets. A method of devising and using transfer function calculation dataset blocks to estimate the either the reliably detectable flaw size or the demonstration flaw size is provided.

Nondestructive evaluation↗

Signal Stabilization of Self-Oscillating Systems

The hunt (self-oscillations) of a physical system may often be removed by the introduction of an appropriate stabilizing signal which changed the open loop gain in a nonlinear manner. More generally, the performance of nonlinear systems in many cases may be improved by the introduction of extra signals. The theory of signal stabilization developed here extends the earlier work by Oldenburger and Liu involving an equivalent gain concept. It is shown that with the aid of the Fourier series the designer can determine the periodic signal to be inserted at one point in a loop to yield a desired stabilizing input to a nonlinear element in the loop. The use of sinusoidal and triangular inputs to a limiter are compared. An example where a limiter is the only nonlinearity is employed to illustrate the theory. The approach developed here explains experimental results previously reported by Oldenburger.

SELF-OSCILLATION↗

Brain signaling and behavioral responses induced by exposure to (56)Fe-particle radiation

Previous experiments have demonstrated that exposure to 56Fe-particle irradiation (1.5 Gy, 1 GeV) produced aging-like accelerations in neuronal and behavioral deficits. Astronauts on long-term space flights will be exposed to similar heavy-particle radiations that might have similar deleterious effects on neuronal signaling and cognitive behavior. Therefore, the present study evaluated whether radiation-induced spatial learning and memory behavioral deficits are associated with region-specific brain signaling deficits by measuring signaling molecules previously found to be essential for behavior [pre-synaptic vesicle proteins, synaptobrevin and synaptophysin, and protein kinases, calcium-dependent PRKCs (also known as PKCs) and PRKA (PRKA RIIbeta)]. The results demonstrated a significant radiation-induced increase in reference memory errors. The increases in reference memory errors were significantly negatively correlated with striatal synaptobrevin and frontal cortical synaptophysin expression. Both synaptophysin and synaptobrevin are synaptic vesicle proteins that are important in cognition. Striatal PRKA, a memory signaling molecule, was also significantly negatively correlated with reference memory errors. Overall, our findings suggest that radiation-induced pre-synaptic facilitation may contribute to some previously reported radiation-induced decrease in striatal dopamine release and for the disruption of the central dopaminergic system integrity and dopamine-mediated behavior.

NASA Discipline Radiation Health↗

Cross-talk in abscisic acid signaling

"Cross-talk" in hormone signaling reflects an organism's ability to integrate different inputs and respond appropriately, a crucial function at the heart of signaling network operation. Abscisic acid (ABA) is a plant hormone involved in bud and seed dormancy, growth regulation, leaf senescence and abscission, stomatal opening, and a variety of plant stress responses. This review summarizes what is known about ABA signaling in the control of stomatal opening and seed dormancy and provides an overview of emerging knowledge about connections between ABA, ethylene, sugar, and auxin synthesis and signaling.

Review, Tutorial↗

Distinct requirements for TrkB and TrkC signaling in target innervation by sensory neurons

Signaling by brain-derived neurotrophic factor (BDNF) via the TrkB receptor, or by neurotrophin-3 (NT3) through the TrkC receptor support distinct populations of sensory neurons. The intracellular signaling pathways activated by Trk (tyrosine kinase) receptors, which in vivo promote neuronal survival and target innervation, are not well understood. Using mice with TrkB or TrkC receptors lacking the docking site for Shc adaptors (trkB(shc/shc) and trkC(shc/shc) mice), we show that TrkB and TrkC promote survival of sensory neurons mainly through Shc site-independent pathways, suggesting that these receptors use similar pathways to prevent apoptosis. In contrast, the regulation of target innervation appears different: in trkB(shc/shc) mice neurons lose target innervation, whereas in trkC(shc/shc) mice the surviving TrkC-dependent neurons maintain target innervation and function. Biochemical analysis indicates that phosphorylation at the Shc site positively regulates autophosphorylation of TrkB, but not of TrkC. Our findings show that although TrkB and TrkC signals mediating survival are largely similar, TrkB and TrkC signals required for maintenance of target innervation in vivo are regulated by distinct mechanisms.

Non-NASA Center↗

Extracellular signaling through the microenvironment: a hypothesis relating carcinogenesis, bystander effects, and genomic instability

Cell growth, differentiation and death are directed in large part by extracellular signaling through the interactions of cells with other cells and with the extracellular matrix; these interactions are in turn modulated by cytokines and growth factors, i.e. the microenvironment. Here we discuss the idea that extracellular signaling integrates multicellular damage responses that are important deterrents to the development of cancer through mechanisms that eliminate abnormal cells and inhibit neoplastic behavior. As an example, we discuss the action of transforming growth factor beta (TGFB1) as an extracellular sensor of damage. We propose that radiation-induced bystander effects and genomic instability are, respectively, positive and negative manifestations of this homeostatic process. Bystander effects exhibited predominantly after a low-dose or a nonhomogeneous radiation exposure are extracellular signaling pathways that modulate cellular repair and death programs. Persistent disruption of extracellular signaling after exposure to relatively high doses of ionizing radiation may lead to the accumulation of aberrant cells that are genomically unstable. Understanding radiation effects in terms of coordinated multicellular responses that affect decisions regarding the fate of a cell may necessitate re-evaluation of radiation dose and risk concepts and provide avenues for intervention.

NASA Discipline Radiation Health↗

The ethylene signal transduction pathway in Arabidopsis

The gaseous hormone ethylene is an important regulator of plant growth and development. Using a simple response of etiolated seedlings to ethylene as a genetic screen, genes involved in ethylene signal transduction have been identified in Arabidopsis. Analysis of two of these genes that have been cloned reveals that ethylene signalling involves a combination of a protein (ETR1) with similarity to bacterial histidine kinases and a protein (CTR1) with similarity to Raf-1, a protein kinase involved in multiple signalling cascades in eukaryotic cells. Several lines of investigation provide compelling evidence that ETR1 encodes an ethylene receptor. For the first time there is a glimpse of the molecular circuitry underlying the signal transduction pathway for a plant hormone.

NASA Discipline Plant Biology↗

Microsurgical removal of epidermal and cortical cells: evidence that the gravitropic signal moves through the outer cell layers in primary roots of maize

There is general agreement that during root gravitropism some sort of growth-modifying signal moves from the cap to the elongation zone and that this signal ultimately induces the curvature that leads to reorientation of the root. However, there is disagreement regarding both the nature of the signal and the pathway of its movement from the root cap to the elongation zone. We examined the pathway of movement by testing gravitropism in primary roots of maize (Zea mays L.) from which narrow (0.5 mm) rings of epidermal and cortical tissue were surgically removed from various positions within the elongation zone. When roots were girdled in the apical part of the elongation zone gravitropic curvature occurred apical to the girdle but not basal to the girdle. Filling the girdle with agar allowed curvature basal to the girdle to occur. Shallow girdles, in which only two or three cell layers (epidermis plus one or two cortical cell layers) were removed, prevented or greatly delayed gravitropic curvature basal to the girdle. The results indicate that the gravitropic signal moves basipetally through the outermost cell layers, perhaps through the epidermis itself.

NASA Discipline Number 29-20↗

Long-distance signaling within Coleus x hybridus leaves; mediated by changes in intra-leaf CO2?

Rapid long-distance signaling in plants can occur via several mechanisms, including symplastic electric coupling and pressure waves. We show here in variegated Coleus leaves a rapid propagation of electrical signals that appears to be caused by changes in intra-leaf CO2 concentrations. Green leaf cells, when illuminated, undergo a rapid depolarization of their membrane potential (Vm) and an increase in their apoplastic pH (pHa) by a process that requires photosynthesis. This is followed by a slower hyperpolarization of Vm and apoplastic acidification, which do not require photosynthesis. White (chlorophyll-lacking) leaf cells, when in isolated white leaf segments, show only the slow response, but when in mixed (i.e. green and white) segments, the rapid Vm depolarization and increase in pHa propagate over more than 10 mm from the green to the white cells. Similarly, these responses propagate 12-20 mm from illuminated to unilluminated green cells. The fact that the propagation of these responses is eliminated when the leaf air spaces are infiltrated with solution indicates that the signal moves in the apoplast rather than the symplast. A depolarization of the mesophyll cells is induced in the dark by a decrease in apoplastic CO2 but not by an increase in pHa. These results support the hypothesis that the propagating signal for the depolarization of the white mesophyll cells is a photosynthetically induced decrease in the CO2 level of the air spaces throughout the leaf.

NASA Discipline Plant Biology↗

Signal processing related to the vestibulo-ocular reflex during combined angular rotation and linear translation of the head

The contributions of vestibular nerve afferents and central vestibular pathways to the angular (AVOR) and linear (LVOR) vestibulo-ocular reflex were studied in squirrel monkeys during fixation of near and far targets. Irregular vestibular afferents did not appear to be necessary for the LVOR, since when they were selectively silenced with galvanic currents the LVOR was essentially unaffected during both far- and near-target viewing. The linear translation signals generated by secondary AVOR neurons in the vestibular nuclei were, on average, in phase with head velocity, inversely related to viewing distance, and were nearly as strong as AVOR-related signals. We suggest that spatial-temporal transformation of linear head translation signals to angular eye velocity commands is accomplished primarily by the addition of viewing distance multiplied, centrally integrated, otolith regular afferent signals to angular VOR pathways.

NASA Discipline Neuroscience↗

In search of cellular control: signal transduction in context

The field of molecular cell biology has experienced enormous advances over the last century by reducing the complexity of living cells into simpler molecular components and binding interactions that are amenable to rigorous biochemical analysis. However, as our tools become more powerful, there is a tendency to define mechanisms by what we can measure. The field is currently dominated by efforts to identify the key molecules and sequences that mediate the function of critical receptors, signal transducers, and molecular switches. Unfortunately, these conventional experimental approaches ignore the importance of supramolecular control mechanisms that play a critical role in cellular regulation. Thus, the significance of individual molecular constituents cannot be fully understood when studied in isolation because their function may vary depending on their context within the structural complexity of the living cell. These higher-order regulatory mechanisms are based on the cell's use of a form of solid-state biochemistry in which molecular components that mediate biochemical processing and signal transduction are immobilized on insoluble cytoskeletal scaffolds in the cytoplasm and nucleus. Key to the understanding of this form of cellular regulation is the realization that chemistry is structure and hence, recognition of the the importance of architecture and mechanics for signal integration and biochemical control. Recent work that has unified chemical and mechanical signaling pathways provides a glimpse of how this form of higher-order cellular control may function and where paths may lie in the future.

Non-NASA Center↗

Decoding Ca2+ signals in plants

Different input signals create their own characteristic Ca2+ fingerprints. These fingerprints are distinguished by frequency, amplitude, duration, and number of Ca2+ oscillations. Ca(2+)-binding proteins and protein kinases decode these complex Ca2+ fingerprints through conformational coupling and covalent modifications of proteins. This decoding of signals can lead to a physiological response with or without changes in gene expression. In plants, Ca(2+)-dependent protein kinases and Ca2+/calmodulin-dependent protein kinases are involved in decoding Ca2+ signals into phosphorylation signals. This review summarizes the elements of conformational coupling and molecular mechanisms of regulation of the two groups of protein kinases by Ca2+ and Ca2+/calmodulin in plants.

Non-NASA Center↗

Real Time Phase Noise Meter Based on a Digital Signal Processor

A digital signal-processing meter for phase noise measurement on sinusoidal signals is dealt with. It enlists a special hardware architecture, made up of a core digital signal processor connected to a data acquisition board, and takes advantage of a quadrature demodulation-based measurement scheme, already proposed by the authors. Thanks to an efficient measurement process and an optimized implementation of its fundamental stages, the proposed meter succeeds in exploiting all hardware resources in such an effective way as to gain high performance and real-time operation. For input frequencies up to some hundreds of kilohertz, the meter is capable both of updating phase noise power spectrum while seamlessly capturing the analyzed signal into its memory, and granting as good frequency resolution as few units of hertz.

phase noise↗