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At least 307 records · Page 17

Association of Diet and Antimicrobial Resistance in Healthy U.S. Adults

Antimicrobial resistance (AMR) represents a significant source of morbidity and mortality worldwide, with expectations that AMR-associated consequences will continue to worsen throughout the coming decades. Since resistance to antibiotics is encoded in the microbiome, interventions aimed at altering the taxonomic composition of the gut might allow us to prophylactically engineer microbiomes that harbor fewer antibiotic resistant genes (ARGs). Diet is one method of intervention, and yet little is known about the association between diet and antimicrobial resistance. To address this knowledge gap, we examined diet using the food frequency questionnaire (FFQ; habitual diet) and 24-h dietary recalls (Automated Self-Administered 24-h [ASA24 ® ] tool) coupled with an analysis of the microbiome using shotgun metagenome sequencing in 290 healthy adult participants of the United States Department of Agriculture (USDA) Nutritional Phenotyping Study. We found that aminoglycosides were the most abundant and prevalent mechanism of AMR in these healthy adults and that aminoglycoside-O-phosphotransferases (aph3-dprime) correlated negatively with total calories and soluble fiber intake. Individuals in the lowest quartile of ARGs (low-ARG) consumed significantly more fiber in their diets than medium- and high-ARG individuals, which was concomitant with increased abundances of obligate anaerobes, especially from the family Clostridiaceae, in their gut microbiota. Finally, we applied machine learning to examine 387 dietary, physiological, and lifestyle features for associations with antimicrobial resistance, finding that increased phylogenetic diversity of diet was associated with low-ARG individuals. These data suggest diet may be a potential method for reducing the burden of AMR.

59 BASIC BIOLOGICAL SCIENCES↗

Characteristics of Veterans With Depression Who Use the Veterans Choice Program of the Veterans Health Administration

Objective: The Veterans Choice Program (VCP) of the Veterans Health Administration (VHA) allowed eligible veterans to use their benefits with participating providers outside the VHA. The authors aimed to identify characteristics of veterans with depression who used or did not use mental health care through the VCP. Methods: In this cross-sectional study, the authors analyzed secondary data from the national VHA Corporate Data Warehouse. VHA administrative data were linked with VCP claims to examine characteristics of VCP-eligible veterans with depression. Further, the study sample included 595,943 unique veterans who were enrolled in the VHA before 2013, were eligible for the VCP in 2016, were alive in 2018, and had an assessed Patient Health Questionnaire–9 (PHQ-9) score or depressive disorder diagnosis documented in the VHA between 2016 and 2018. Results: Veterans who used the VCP had lower medical comorbidity scores and lived in less socioeconomically disadvantaged counties, compared with veterans who received only VHA care. VCP veterans were also more likely to have a PHQ-9 score assessment and to have higher mean depression scores. Mean counts of annual mental health visits per 1,000 veterans were markedly higher for direct VHA care than for care provided via the VCP. As a percentage of the total counts of visits per 1,000 veterans across the VCP and VHA, residential programs and outpatient procedures were the services that were most frequently delivered through the VCP. Conclusions: Between 2016 and 2018, the VCP was used primarily to augment mental health care provided by the VHA, rather than to fill a gap in care.

60 APPLIED LIFE SCIENCES↗

The 6-minute walk test predicts mortality in a pulmonary nontuberculous mycobacteria-predominant bronchiectasis cohort

Background: Bronchiectasis is a chronic lung condition frequently associated with nontuberculous mycobacteria pulmonary (NTM) disease. Persons with these conditions are at increased risk of mortality. Patient reported outcome (PRO) instruments and the 6-minute walk test (6MWT) have been shown to predict mortality for several lung conditions, but these measures have not been fully evaluated for bronchiectasis and NTM. Methods: We conducted a retrospective cohort study among adult patients enrolled in a natural history study of bronchiectasis at the National Heart, Lung, and Blood Institute. Electronic medical records were queried for demographic, clinical, microbiologic, radiographic, and PRO instrument data: St. George’s Respiratory Questionnaire (SGRQ), Medical Research Council Dyspnea Scale, and the Pulmonary Symptom Severity Score (PSSS). The study baseline date was defined as the patient’s first visit after January 1st, 2015 with a SGRQ or 6MWT completed. Follow-up was defined as the interval between the study baseline visit and date of death or December 31st, 2019. Sex-stratified Cox proportional-hazards regression was conducted to identify predictors of mortality. Separate models were run for each PRO and 6MWT measure, controlling for age, body mass index (BMI), fibrocavitary disease status, and M. abscessus infection. Results: In multivariable Cox proportional-hazards regression models, the PSSS-severity (aHR 1.29, 95% CI 1.04–1.59), the 6MWT total distance walked (aHR 0.938, 95% CI 0.896–0.981) and distance saturation product (aHR 0.930, 95% CI 0.887–0.974) independently predicted mortality. In addition, BMI was significantly predictive of mortality in all models. Conclusions: The 6MWT and a PRO instrument capturing symptom severity are independently predictive of mortality in our cohort of bronchiectasis patients.

6-minute walk test↗

Association of chronic fatigue syndrome with premature telomere attrition

Background: Chronic fatigue syndrome (CFS), also known as myalgic encephalomyelitis (ME), is a severely debilitating condition of unknown etiology. The symptoms and risk factors of ME/CFS share features of accelerated aging implicated in several diseases. Using telomere length as a marker, this study was performed to test the hypothesis that ME/CFS is associated with accelerated aging. Methods: Participant (n = 639) data came from the follow-up time point of the Georgia CFS surveillance study. Using the 1994 CFS Research Case Definition with questionnaire-based subscale thresholds for fatigue, function, and symptoms, participants were classified into four illness groups: CFS if all criteria were met (n = 64), CFS-X if CFS with exclusionary conditions (n = 77), ISF (insufficient symptoms/fatigue) if only some criteria were met regardless of exclusionary conditions (n = 302), and NF (non-fatigued) if no criteria and no exclusionary conditions (n = 196). Relative telomere length (T/S ratio) was measured using DNA from whole blood and real-time PCR. General linear models were used to estimate the association of illness groups or T/S ratio with demographics, biological measures and covariates with significance set at p < 0.05. Results: The mean T/S ratio differed significantly by illness group (p = 0.0017); the T/S ratios in CFS (0.90 ± 0.03) and ISF (0.94 ± 0.02) were each significantly lower than in NF (1.06 ± 0.04). Differences in T/S ratio by illness groups remained significant after adjustment for covariates of age, sex, body mass index, waist–hip ratio, post-exertional malaise and education attainment. Telomere length was shorter by 635, 254 and 424 base pairs in CFS, CFS-X and ISF, respectively, compared to NF. This shorter telomere length translates to roughly 10.1–20.5, 4.0–8.2 and 6.6–13.7 years of additional aging in CFS, CFS-X and ISF compared to NF respectively. Further, stratified analyses based on age and sex demonstrated that the association of ME/CFS with short telomeres is largely moderated by female subjects < 45 years old. Conclusions: This study found a significant association of ME/CFS with premature telomere attrition that is largely moderated by female subjects < 45 years old. Our results indicate that ME/CFS could be included in the list of conditions associated with accelerated aging. Further work is needed to evaluate the functional significance of accelerated aging in ME/CFS.

60 APPLIED LIFE SCIENCES↗

Impact of Long-Course Neoadjuvant Radiation on Postoperative Low Anterior Resection Syndrome and Quality of Life in Rectal Cancer: Post Hoc Analysis of a Randomized Controlled Trial

Background: Neoadjuvant radiation is recommended for locally advanced rectal cancer, with proven benefit in local control but not in disease-free survival. However, the impact of long-course radiation on postoperative bowel function and quality of life (QOL) remains controversial. This study aimed to investigate the impact of long-course neoadjuvant radiation on bowel function and QOL, and to identify risk factors for severe bowel dysfunction. Methods: Patients who underwent long-course neoadjuvant chemoradiotherapy (nCRT) or chemotherapy (nCT) followed by radical low anterior resection for locally advanced rectal cancer were recruited from the FOWARC randomized controlled trial. Low anterior resection syndrome (LARS) score and European Organisation for Research and Treatment of Cancer (EORTC) C30/CR29 questionnaires were used to assess bowel function and QOL, respectively. Results: Overall, 220 patients responded after a median follow-up of 40.2 months, of whom 119 (54.1%) reported major LARS, 74 (33.6%) reported minor LARS, and 27 (12.3%) reported no LARS. Compared with the nCT group, the nCRT group reported more major LARS (64.4% vs. 38.6%, p < 0.001) and worse QOL. Long-course neoadjuvant radiation (OR 2.20, 95% CI 1.24–3.91; p = 0.007), height of anastomosis (OR 0.74, 95% CI 0.63–0.88; p < 0.001), and diverting ileostomy (OR 2.59, 95% CI 1.27–5.30; p = 0.009) were independent risk factors for major LARS. Conclusions: Long-course neoadjuvant radiation, along with low anastomosis, are likely independent risk factors for postoperative bowel function and QOL. Our findings might have implications for alleviating LARS and improving QOL by informing selection of neoadjuvant treatment.

62 RADIOLOGY AND NUCLEAR MEDICINE↗

Lead Exposure during Early Human Development and DNA Methylation of Imprinted Gene Regulatory Elements in Adulthood

Here, lead exposure during early development causes neurodevelopmental disorders by unknown mechanisms. Epidemiologic studies have focused recently on determining associations between lead exposure and global DNA methylation; however, such approaches preclude the identification of loci that may alter human disease risk. The objective of this study was to determine whether maternal, postnatal, and early childhood lead exposure can alter the differentially methylated regions (DMRs) that control the monoallelic expression of imprinted genes involved in metabolism, growth, and development. Questionnaire data and serial blood lead levels were obtained from 105 participants (64 females, 41 males) of the Cincinnati Lead Study from birth to 78 months. When participants were adults, we used Sequenom EpiTYPER assays to test peripheral blood DNA to quantify CpG methylation in peripheral blood leukocytes at DMRs of 22 human imprinted genes. Statistical analyses were conducted using linear regression. Mean blood lead concentration from birth to 78 months was associated with a significant decrease in PEG3 DMR methylation (β = –0.0014; 95% CI: –0.0023, –0.0005, p = 0.002), stronger in males (β = –0.0024; 95% CI: –0.0038, –0.0009, p = 0.003) than in females (β = –0.0009; 95% CI: –0.0020, 0.0003, p = 0.1). Elevated mean childhood blood lead concentration was also associated with a significant decrease in IGF2/H19 (β = –0.0013; 95% CI: –0.0023, –0.0003, p = 0.01) DMR methylation, but primarily in females, (β = –0.0017; 95% CI: –0.0029, –0.0006, p = 0.005) rather than in males, (β = –0.0004; 95% CI: –0.0023, 0.0015, p = 0.7). Elevated blood lead concentration during the neonatal period was associated with higher PLAGL1/HYMAI DMR methylation regardless of sex (β = 0.0075; 95% CI: 0.0018, 0.0132, p = 0.01). The magnitude of associations between cumulative lead exposure and CpG methylation remained unaltered from 30 to 78 months. Our findings provide evidence that early childhood lead exposure results in sexdependent and gene-specific DNA methylation differences in the DMRs of PEG3, IGF2/H19, and PLAGL1/HYMAI in adulthood.

59 BASIC BIOLOGICAL SCIENCES↗

Spatial Access Priority Mapping (SAPM) with Fishers: A Quantitative GIS Method for Participatory Planning

Spatial management tools, such as marine spatial planning and marine protected areas, are playing an increasingly important role in attempts to improve marine management and accommodate conflicting needs. Robust data are needed to inform decisions among different planning options, and early inclusion of stakeholder involvement is widely regarded as vital for success. One of the biggest stakeholder groups, and the most likely to be adversely impacted by spatial restrictions is the fishing community. In order to take their priorities into account, planners need to understand spatial variation in their perceived value of the sea. Here, a readily accessible, novel method for quantitatively mapping fishers’ spatial access priorities is presented. Spatial access priority mapping, or SAPM, uses only basic functions of standard spreadsheet and GIS software. Unlike the use of remote-sensing data, SAPM actively engages fishers in participatory mapping, documenting rather than inferring their priorities. By so doing, SAPM also facilitates the gathering of other useful data, such as local ecological knowledge. The method was tested and validated in Northern Ireland, where over 100 fishers participated in a semi-structured questionnaire and mapping exercise. The response rate was excellent, 97%, demonstrating fishers’ willingness to be involved. The resultant maps are easily accessible and instantly informative, providing a very clear visual indication of which areas are most important for the fishers. The maps also provide quantitative data, which can be used to analyse the relative impact of different management options on the fishing industry and can be incorporated into planning software, such as MARXAN, to ensure that conservation goals can be met at minimum negative impact to the industry. This research shows how spatial access priority mapping can facilitate the early engagement of fishers and the ready incorporation of their priorities into the decision-making process in a transparent, quantitative way.

54 ENVIRONMENTAL SCIENCES↗

Inadequate Exercise as a Risk Factor for Sepsis Mortality

Objective: Test whether inadequate exercise is related to sepsis mortality. Research Design and Methods: Mortality surveillance of an epidemiological cohort of 155,484 National Walkers’ and Runners’ Health Study participants residing in the United States. Deaths were monitored for an average of 11.6-years using the National Death index through December 31, 2008. Cox proportional hazard analyses were used to compare sepsis mortality (ICD-10 A40-41) to inadequate exercise (,1.07 METh/d run or walked) as measured on their baseline questionnaires. Deaths occurring within one year of the baseline survey were excluded. Results: Sepsis was the underlying cause in 54 deaths (sepsisunderlying) and a contributing cause in 184 deaths (sepsiscontributing), or 238 total sepsis-related deaths (sepsistotal). Inadequate exercise was associated with 2.24-fold increased risk for sepsisunderlying (95%CI: 1.21 to 4.07-fold, P = 0.01), 2.11-fold increased risk for sepsiscontributing (95%CI: 1.51- to 2.92-fold, P,1024 ), and 2.13-fold increased risk for sepsistotal (95%CI: 1.59- to 2.84-fold, P,1026 ) when adjusted for age, sex, race, and cohort. The risk increase did not differ significantly between runners and walkers, by sex, or by age. Sepsistotal risk was greater in diabetics (P = 1025 ), cancer survivors (P = 0.0001), and heart attack survivors (P = 0.003) and increased with waist circumference (P = 0.0004). The sepsistotal risk associated with inadequate exercise persisted when further adjusted for diabetes, prior cancer, prior heart attack and waist circumference, and when excluding deaths with cancer, or cardiovascular, respiratory, or genitourinary disease as the underlying cause. Inadequate exercise also increased sepsistotal risk in 2163 baseline diabetics (4.78-fold, 95%CI: 2.1- to 13.8-fold, P = 0.0001) when adjusted, which was significantly greater (P = 0.03) than the adjusted risk increase in non-diabetics (1.80-fold, 95%CI: 1.30- to 2.46-fold, P = 0.0006). Conclusion: Inadequate exercise is a risk factor for sepsis mortality, particular in diabetics.

60 APPLIED LIFE SCIENCES↗

Neural Correlates of Gender Differences in Reputation Building

Gender differences in cooperative choices and their neural correlates were investigated in a situation where reputation represented a crucial issue. Males and females were involved in an economic exchange (trust game) where economic and reputational payoffs had to be balanced in order to increase personal welfare. At the behavioral level, females showed a stronger reaction to negative reputation judgments that led to higher cooperation than males, measured by back transfers in the game. The neuroanatomical counterpart of this gender difference was found within the reward network (engaged in producing expectations of positive results) and reputation-related brain networks, such as the self-control network (engaged in strategically resisting the temptation to defect) and the mentalizing network (engaged in thinking about how one is viewed by others), in which the dorsolateral prefrontal cortex (DLPFC) and the medial (M)PFC respectively play a crucial role. Furthermore, both DLPFC and MPFC activity correlated with the amount of back transfer, as well as with the personality dimensions assessed with the Big-Five Questionnaire (BFQ-2). Males, according to their greater DLPFC recruitment and their higher level of the BFQ-2 subscale of Dominance, were more focused on implementing a profit-maximizing strategy, pursuing this target irrespectively of others’ judgments. On the contrary, females, according to their greater MPFC activity and their lower level of Dominance, were more focused on the reputation per se and not on the strategic component of reputation building. These findings shed light on the sexual dimorphism related to cooperative behavior and its neural correlates.

59 BASIC BIOLOGICAL SCIENCES↗

Sedentary behavior associated with reduced medial temporal lobe thickness in middle-aged and older adults

Atrophy of the medial temporal lobe (MTL) occurs with aging, resulting in impaired episodic memory. Aerobic fitness is positively correlated with total hippocampal volume, a heavily studied memory-critical region within the MTL. However, research on associations between sedentary behavior and MTL subregion integrity is limited. Here we explore associations between thickness of the MTL and its subregions (namely CA1, CA23DG, fusiform gyrus, subiculum, parahippocampal, perirhinal and entorhinal cortex,), physical activity, and sedentary behavior. We assessed 35 non-demented middle-aged and older adults (25 women, 10 men; 45–75 years) using the International Physical Activity Questionnaire for older adults, which quantifies physical activity levels in MET-equivalent units and asks about the average number of hours spent sitting per day. All participants had high resolution MRI scans performed on a Siemens Allegra 3T MRI scanner, which allows for detailed investigation of the MTL. Controlling for age, total MTL thickness correlated inversely with hours of sitting/day (r = -0.37, p = 0.03). In MTL subregion analysis, parahippocampal (r = -0.45, p = 0.007), entorhinal (r = -0.33, p = 0.05) cortical and subiculum (r = -0.36, p = .04) thicknesses correlated inversely with hours of sitting/day. No significant correlations were observed between physical activity levels and MTL thickness. Though preliminary, our results suggest that more sedentary non-demented individuals have less MTL thickness. Future studies should include longitudinal analyses and explore mechanisms, as well as the efficacy of decreasing sedentary behaviors to reverse this association.

60 APPLIED LIFE SCIENCES↗

Feasibility and preliminary efficacy for morning bright light therapy to improve sleep and plasma biomarkers in US Veterans with TBI. A prospective, open-label, single-arm trial

Mild traumatic brain injury (TBI) is associated with persistent sleep-wake dysfunction, including insomnia and circadian rhythm disruption, which can exacerbate functional outcomes including mood, pain, and quality of life. Present therapies to treat sleep-wake disturbances in those with TBI (e.g., cognitive behavioral therapy for insomnia) are limited by marginal efficacy, poor patient acceptability, and/or high patient/provider burden. Thus, this study aimed to assess the feasibility and preliminary efficacy of morning bright light therapy, to improve sleep in Veterans with TBI (NCT03578003). Thirty-three Veterans with history of TBI were prospectively enrolled in a single-arm, open-label intervention using a lightbox (~10,000 lux at the eye) for 60-minutes every morning for 4-weeks. Pre- and post-intervention outcomes included questionnaires related to sleep, mood, TBI, post-traumatic stress disorder (PTSD), and pain; wrist actigraphy as a proxy for objective sleep; and blood-based biomarkers related to TBI/sleep. The protocol was rated favorably by ~75% of participants, with adherence to the lightbox and actigraphy being ~87% and 97%, respectively. Post-intervention improvements were observed in self-reported symptoms related to insomnia, mood, and pain; actigraphy-derived measures of sleep; and blood-based biomarkers related to peripheral inflammatory balance. The severity of comorbid PTSD was a significant positive predictor of response to treatment. Morning bright light therapy is a feasible and acceptable intervention that shows preliminary efficacy to treat disrupted sleep in Veterans with TBI. A full-scale randomized, placebo-controlled study with longitudinal follow-up is warranted to assess the efficacy of morning bright light therapy to improve sleep, biomarkers, and other TBI related symptoms.

60 APPLIED LIFE SCIENCES↗

2019 and 2022 Twin Cities Travel Behavior Inventory Surveys

# 2019 and 2022 Twin Cities Travel Behavior Inventory Surveys To help local and regional planning agencies understand shifting demographics and travel patterns, surveys were conducted in Minnesota’s greater Twin Cities region in 2019 and 2022. Survey results aided the Metropolitan Council in proposing practical transportation investments, preparing competitive grant applications, and prioritizing improvements to best fit regional needs. ## Data Collection Agency RSG conducted the surveys for the Metropolitan Council. ## Survey Methodology These mixed-mode surveys focused on bus, rail, car, micromobility, ride-hailing, and walking. Designed as household travel surveys, they were carried out in English, Spanish, Karen, Oromo, Somali, and Hmong during two timeframes: Oct. 1, 2018—Sept. 30, 2019 and June 22, 2021—Feb. 5, 2022. Participants accessed the surveys using a smartphone-based app, website, or call center. A questionnaire captured data about demographics, daily travel activities, and typical transportation patterns to inform model updates and gain information about emerging behavioral changes such as electric vehicle adoption and teleworking frequency. It also addressed the impacts of COVID-19 on participants’ typical travel behavior. ## Survey Records, Data, and Documentation Survey records include a total of 31,251 participants—16,152 participants from 7,837 households during the 2019 survey and 15,099 participants from 7,952 households during the 2022 survey.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗

2019 and 2022 Twin Cities Travel Behavior Inventory Surveys

# 2019 and 2022 Twin Cities Travel Behavior Inventory Surveys To help local and regional planning agencies understand shifting demographics and travel patterns, surveys were conducted in Minnesota’s greater Twin Cities region in 2019 and 2022. Survey results aided the Metropolitan Council in proposing practical transportation investments, preparing competitive grant applications, and prioritizing improvements to best fit regional needs. ## Data Collection Agency RSG conducted the surveys for the Metropolitan Council. ## Survey Methodology These mixed-mode surveys focused on bus, rail, car, micromobility, ride-hailing, and walking. Designed as household travel surveys, they were carried out in English, Spanish, Karen, Oromo, Somali, and Hmong during two timeframes: Oct. 1, 2018—Sept. 30, 2019 and June 22, 2021—Feb. 5, 2022. Participants accessed the surveys using a smartphone-based app, website, or call center. A questionnaire captured data about demographics, daily travel activities, and typical transportation patterns to inform model updates and gain information about emerging behavioral changes such as electric vehicle adoption and teleworking frequency. It also addressed the impacts of COVID-19 on participants’ typical travel behavior. ## Survey Records, Data, and Documentation Survey records include a total of 31,251 participants—16,152 participants from 7,837 households during the 2019 survey and 15,099 participants from 7,952 households during the 2022 survey.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗

2019 and 2022 Twin Cities Travel Behavior Inventory Surveys

# 2019 and 2022 Twin Cities Travel Behavior Inventory Surveys To help local and regional planning agencies understand shifting demographics and travel patterns, surveys were conducted in Minnesota’s greater Twin Cities region in 2019 and 2022. Survey results aided the Metropolitan Council in proposing practical transportation investments, preparing competitive grant applications, and prioritizing improvements to best fit regional needs. ## Data Collection Agency RSG conducted the surveys for the Metropolitan Council. ## Survey Methodology These mixed-mode surveys focused on bus, rail, car, micromobility, ride-hailing, and walking. Designed as household travel surveys, they were carried out in English, Spanish, Karen, Oromo, Somali, and Hmong during two timeframes: Oct. 1, 2018—Sept. 30, 2019 and June 22, 2021—Feb. 5, 2022. Participants accessed the surveys using a smartphone-based app, website, or call center. A questionnaire captured data about demographics, daily travel activities, and typical transportation patterns to inform model updates and gain information about emerging behavioral changes such as electric vehicle adoption and teleworking frequency. It also addressed the impacts of COVID-19 on participants’ typical travel behavior. ## Survey Records, Data, and Documentation Survey records include a total of 31,251 participants—16,152 participants from 7,837 households during the 2019 survey and 15,099 participants from 7,952 households during the 2022 survey.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗

2019 and 2022 Twin Cities Travel Behavior Inventory Surveys

# 2019 and 2022 Twin Cities Travel Behavior Inventory Surveys To help local and regional planning agencies understand shifting demographics and travel patterns, surveys were conducted in Minnesota’s greater Twin Cities region in 2019 and 2022. Survey results aided the Metropolitan Council in proposing practical transportation investments, preparing competitive grant applications, and prioritizing improvements to best fit regional needs. ## Data Collection Agency RSG conducted the surveys for the Metropolitan Council. ## Survey Methodology These mixed-mode surveys focused on bus, rail, car, micromobility, ride-hailing, and walking. Designed as household travel surveys, they were carried out in English, Spanish, Karen, Oromo, Somali, and Hmong during two timeframes: Oct. 1, 2018—Sept. 30, 2019 and June 22, 2021—Feb. 5, 2022. Participants accessed the surveys using a smartphone-based app, website, or call center. A questionnaire captured data about demographics, daily travel activities, and typical transportation patterns to inform model updates and gain information about emerging behavioral changes such as electric vehicle adoption and teleworking frequency. It also addressed the impacts of COVID-19 on participants’ typical travel behavior. ## Survey Records, Data, and Documentation Survey records include a total of 31,251 participants—16,152 participants from 7,837 households during the 2019 survey and 15,099 participants from 7,952 households during the 2022 survey.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗

2019 and 2022 Twin Cities Travel Behavior Inventory Surveys

# 2019 and 2022 Twin Cities Travel Behavior Inventory Surveys To help local and regional planning agencies understand shifting demographics and travel patterns, surveys were conducted in Minnesota’s greater Twin Cities region in 2019 and 2022. Survey results aided the Metropolitan Council in proposing practical transportation investments, preparing competitive grant applications, and prioritizing improvements to best fit regional needs. ## Data Collection Agency RSG conducted the surveys for the Metropolitan Council. ## Survey Methodology These mixed-mode surveys focused on bus, rail, car, micromobility, ride-hailing, and walking. Designed as household travel surveys, they were carried out in English, Spanish, Karen, Oromo, Somali, and Hmong during two timeframes: Oct. 1, 2018—Sept. 30, 2019 and June 22, 2021—Feb. 5, 2022. Participants accessed the surveys using a smartphone-based app, website, or call center. A questionnaire captured data about demographics, daily travel activities, and typical transportation patterns to inform model updates and gain information about emerging behavioral changes such as electric vehicle adoption and teleworking frequency. It also addressed the impacts of COVID-19 on participants’ typical travel behavior. ## Survey Records, Data, and Documentation Survey records include a total of 31,251 participants—16,152 participants from 7,837 households during the 2019 survey and 15,099 participants from 7,952 households during the 2022 survey.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗

2019 and 2022 Twin Cities Travel Behavior Inventory Surveys

# 2019 and 2022 Twin Cities Travel Behavior Inventory Surveys To help local and regional planning agencies understand shifting demographics and travel patterns, surveys were conducted in Minnesota’s greater Twin Cities region in 2019 and 2022. Survey results aided the Metropolitan Council in proposing practical transportation investments, preparing competitive grant applications, and prioritizing improvements to best fit regional needs. ## Data Collection Agency RSG conducted the surveys for the Metropolitan Council. ## Survey Methodology These mixed-mode surveys focused on bus, rail, car, micromobility, ride-hailing, and walking. Designed as household travel surveys, they were carried out in English, Spanish, Karen, Oromo, Somali, and Hmong during two timeframes: Oct. 1, 2018—Sept. 30, 2019 and June 22, 2021—Feb. 5, 2022. Participants accessed the surveys using a smartphone-based app, website, or call center. A questionnaire captured data about demographics, daily travel activities, and typical transportation patterns to inform model updates and gain information about emerging behavioral changes such as electric vehicle adoption and teleworking frequency. It also addressed the impacts of COVID-19 on participants’ typical travel behavior. ## Survey Records, Data, and Documentation Survey records include a total of 31,251 participants—16,152 participants from 7,837 households during the 2019 survey and 15,099 participants from 7,952 households during the 2022 survey.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗

2019 and 2022 Twin Cities Travel Behavior Inventory Surveys

# 2019 and 2022 Twin Cities Travel Behavior Inventory Surveys To help local and regional planning agencies understand shifting demographics and travel patterns, surveys were conducted in Minnesota’s greater Twin Cities region in 2019 and 2022. Survey results aided the Metropolitan Council in proposing practical transportation investments, preparing competitive grant applications, and prioritizing improvements to best fit regional needs. ## Data Collection Agency RSG conducted the surveys for the Metropolitan Council. ## Survey Methodology These mixed-mode surveys focused on bus, rail, car, micromobility, ride-hailing, and walking. Designed as household travel surveys, they were carried out in English, Spanish, Karen, Oromo, Somali, and Hmong during two timeframes: Oct. 1, 2018—Sept. 30, 2019 and June 22, 2021—Feb. 5, 2022. Participants accessed the surveys using a smartphone-based app, website, or call center. A questionnaire captured data about demographics, daily travel activities, and typical transportation patterns to inform model updates and gain information about emerging behavioral changes such as electric vehicle adoption and teleworking frequency. It also addressed the impacts of COVID-19 on participants’ typical travel behavior. ## Survey Records, Data, and Documentation Survey records include a total of 31,251 participants—16,152 participants from 7,837 households during the 2019 survey and 15,099 participants from 7,952 households during the 2022 survey.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗