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At least 307 records · Page 17

Seasonal stabilization effects slowed the greening of the Northern Hemisphere over the last two decades

Rising atmospheric CO₂ and warming spring temperatures increase vegetation growth and the terrestrial carbon sink. However, drought, heat stress, phenology, and resource limitations may stabilize or limit theses projected increases. We investigate the balance between these amplifying and stabilizing ecological factors by asking whether enhanced early-season growth leads to continued late-season growth. Using the Moderate Resolution Imaging Spectroradiometer (MODIS) leaf area index (LAI) dataset, we identify three seasonal growth patterns based on early- and peak-season positive LAI anomalies: (1) amplification, where late-season LAI anomalies exceed earlier ones; (2) weak stabilization, where late-season anomalies remain similar or slightly lower; and (3) strong stabilization, where late-season anomalies become negative. Weak and strong stabilization events dominate across 67% and 26% of Northern Hemisphere ecosystems above 30°N, respectively. The absence of any trend in amplifying or stabilizing events suggests stabilizing factors seasonally offset CO₂ and temperature-induced spring greening. Terrestrial biosphere models underestimate strong stabilization and overestimate amplification events. This inconsistency arises from the models’ underestimation late-season LAI sensitivity to precipitation in water-limited regions; overlook negative legacy effects of early enhanced LAI on late-season soil moisture via evapotranspiration losses in energy-limited regions. Our findings suggest water/heat stress and resource limitations limit greening and the land carbon sink.

Zhang, Wen↗

The ones that got away: chemical tagging of globular cluster-origin stars with Gaia BP/RP spectra

ABSTRACT Globular clusters (GCs) are sites of extremely efficient star formation, and recent studies suggest they significantly contributed to the early Milky Way’s stellar mass build-up. Although their role has since diminished, GCs’ impact on the Galaxy’s initial evolution can be traced today by identifying their most chemically unique stars – those with anomalous nitrogen and aluminum overabundances and oxygen depletion. While they are a perfect tracer of clusters, be it intact or fully dissolved, these high-[N/O], high-[Al/Fe] GC-origin stars are extremely rare within the current Galaxy. To address the scarcity of these unusual, precious former GC members, we train a neural network (NN) to identify high-[N/O], high-[Al/Fe] stars using low-resolution Gaia Blue Photometer/Red Photometer spectra. Our NN achieves a classification accuracy of approximately $\approx 99~{{\ \rm per\ cent}}$ and a false positive rate of around $\approx 7~{{\ \rm per\ cent}}$, identifying 878 new candidates in the Galactic field. We validate our results with several physically motivated sanity checks, showing, for example, that the incidence of selected stars in Galactic GCs is significantly higher than in the field. Moreover, we find that most of our GC-origin candidates reside in the inner Galaxy, having likely formed in the proto-Milky Way, consistent with previous research. The fraction of GC candidates in the field drops at a metallicity of [Fe/H]$\approx -1$, approximately coinciding with the completion of spin-up, i.e. the formation of the Galactic stellar disc.

Kane, Sarah G. (ORCID:0000000184111012)↗

Regioselectivity mechanism of the Thunbergia alata Δ 6 -16:0-acyl carrier protein desaturase

Plant plastidial acyl–acyl carrier protein (ACP) desaturases are a soluble class of diiron-containing enzymes that are distinct from the diiron-containing integral membrane desaturases found in plants and other organisms. The archetype of this class is the stearoyl-ACP desaturase which converts stearoyl-ACP into oleoyl (18:1Δ 9 cis )-ACP. Several variants expressing distinct regioselectivity have been described including a Δ 6 -16:0-ACP desaturase from black-eyed Susan vine ( Thunbergia alata ). We solved a crystal structure of the T. alata desaturase at 2.05 Å resolution. Using molecular dynamics (MD) simulations, we identified a low-energy complex between 16:0-ACP and the desaturase that would position C6 and C7 of the acyl chain adjacent to the diiron active site. The model complex was used to identify mutant variants that could convert the T. alata Δ 6 desaturase to Δ 9 regioselectivity. Additional modeling between ACP and the mutant variants confirmed the predicted regioselectivity. Furthermore, to validate the in-silico predictions, we synthesized two variants of the T. alata desaturase and analyzed their reaction products using gas chromatography-coupled mass spectrometry. Assay results confirmed that mutants designed to convert T. alata Δ 6 to Δ 9 selectivity exhibited the predicted changes. In complementary experiments, variants of the castor desaturase designed to convert Δ 9 to Δ 6 selectivity lost some of their Δ9 desaturation ability and gained the ability to desaturate at the Δ 6 position. The computational workflow for revealing the mechanistic understanding of regioselectivity presented herein lays a foundation for designing acyl-ACP desaturases with novel selectivities to increase the diversity of monoenes available for bioproduct applications.

59 BASIC BIOLOGICAL SCIENCES↗

Self-compensating light calorimetry with liquid argon time projection chamber for GeV neutrino physics

The Liquid Argon Time Projection Chamber (LArTPC) is a powerful dual calorimeter capable of estimating particle energy from both ionization charge and scintillation light. Here, our study shows that, due to the recombination luminescence, the LArTPC functions as a self-compensating light calorimeter: the missing energy in the hadronic component is compensated for by the increased luminescence relative to the electromagnetic component. Using 0.5-5 GeV electron neutrino charged current interactions as a case study, we show that good compensation of the electron-to-hadron response ratio (e/h) from 1-1.05 can be achieved across a broad range of drift electric fields (0.2-1.8 kV / cm), with better performance for neutrino energies above 2 GeV. This study highlights the potential of light calorimetry in LArTPCs for GeV neutrino energy reconstruction, complementing traditional charge calorimetry. Under ideal conditions of uniform light collection, we show that LArTPC light calorimetry can achieve an energy resolution comparable to the charge imaging calorimetry. Challenges arising from nonuniform light collection in large LArTPCs can be mitigated with a position-dependent light yield correction derived from 3D charge signal imaging.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗

Evaluation of interactive and prescribed agricultural ammonia emissions for simulating atmospheric composition in CAM-chem

Abstract. Ammonia (NH3) plays a central role in the chemistry of inorganic secondary aerosols in the atmosphere. The largest emission sector for NH3 is agriculture, where NH3 is volatilized from livestock wastes and fertilized soils. Although the NH3 volatilization from soils is driven by the soil temperature and moisture, many atmospheric chemistry models prescribe the emission using yearly emission inventories and climatological seasonal variations. Here we evaluate an alternative approach where the NH3 emissions from agriculture are simulated interactively using the process model FANv2 (Flow of Agricultural Nitrogen, version 2) coupled to the Community Atmospheric Model with Chemistry (CAM-chem). We run a set of 6-year global simulations using the NH3 emission from FANv2 and three global emission inventories (EDGAR, CEDS and HTAP) and evaluate the model performance using a global set of multi-component (atmospheric NH3 and NH4+, and NH4+ wet deposition) in situ observations. Over East Asia, Europe and North America, the simulations with different emissions perform similarly when compared with the observed geographical patterns. The seasonal distributions of NH3 emissions differ between the inventories, and the comparison to observations suggests that both FANv2 and the inventories would benefit from more realistic timing of fertilizer applications. The largest differences between the simulations occur over data-scarce regions. In Africa, the emissions simulated by FANv2 are 200 %–300 % higher than in the inventories, and the available in situ observations from western and central Africa, as well as NH3 retrievals from the Infrared Atmospheric Sounding Interferometer (IASI) instrument, are consistent with the higher NH3 emissions as simulated by FANv2. Overall, in simulating ammonia and ammonium concentrations over regions with detailed regional emission inventories, the inventories based on these details (HTAP, CEDS) capture the atmospheric concentrations and their seasonal variability the best. However these inventories cannot capture the impact of meteorological variability on the emissions, nor can these inventories couple the emissions to the biogeochemical cycles and their changes with climate drivers. Finally, we show with sensitivity experiments that the simulated time-averaged nitrate concentration in air is sensitive to the temporal resolution of the NH3 emissions. Over the CASTNET monitoring network covering the US, resolving the NH3 emissions hourly instead monthly reduced the positive model bias from approximately 80 % to 60 % of the observed yearly mean nitrate concentration. This suggests that some of the commonly reported overestimation of aerosol nitrate over the US may be related to unresolved temporal variability in the NH3 emissions.

54 ENVIRONMENTAL SCIENCES↗

Improved Localization Precision and Angular Resolution of a Cylindrical, Time-Encoded Imaging System From Adaptive Detector Movements

To the first order, the localization precision and angular resolution of a cylindrical, time-encoded imaging (c-TEI) system is governed by the geometry of the system. Improving either measure requires increasing the mask radius or decreasing the detector diameter, both of which are undesirable. Here, we propose an alternative option of repositioning the detector within the mask to increase the detector-to-mask distance in the direction of a source, thereby improving the localization precision and angular resolution in that direction. Since the detector-to-mask distance only increases for a small portion of the field of view (FOV), we propose implementing adaptive imaging where one leverages data collected during the measurement to optimize the system configuration. This article utilizes both simulations and experiments to set upper bounds on the potential gain from adaptive detector movements for one and two sources in the FOV. When only one source is present, adaptive detector movements can improve the localization precision and angular resolution by 20% for a source at 90 cm and by 32% for a far-field source. When two sources are present, adaptive detector movements can improve localization precision and angular resolution by up to 50% for sources that are ~10° apart (90 cm from the system). We experimentally verify these results through maximum likelihood estimation of the source position(s) and image reconstruction of point sources that are close together. As a demonstration of an adaptive imaging algorithm, we image a complex arrangement of special nuclear material at the Zero Power Physics Reactor facility at Idaho National Laboratory.

46 - INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AN↗

Crystallographic structure of wild-type SARS-CoV-2 main protease acyl-enzyme intermediate with physiological C-terminal autoprocessing site

Abstract Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), the pathogen that causes the disease COVID-19, produces replicase polyproteins 1a and 1ab that contain, respectively, 11 or 16 nonstructural proteins (nsp). Nsp5 is the main protease (M pro ) responsible for cleavage at eleven positions along these polyproteins, including at its own N- and C-terminal boundaries, representing essential processing events for subsequent viral assembly and maturation. We have determined X-ray crystallographic structures of this cysteine protease in its wild-type free active site state at 1.8 Å resolution, in its acyl-enzyme intermediate state with the native C-terminal autocleavage sequence at 1.95 Å resolution and in its product bound state at 2.0 Å resolution by employing an active site mutation (C145A). We characterize the stereochemical features of the acyl-enzyme intermediate including critical hydrogen bonding distances underlying catalysis in the Cys/His dyad and oxyanion hole. We also identify a highly ordered water molecule in a position compatible for a role as the deacylating nucleophile in the catalytic mechanism and characterize the binding groove conformational changes and dimerization interface that occur upon formation of the acyl-enzyme. Collectively, these crystallographic snapshots provide valuable mechanistic and structural insights for future antiviral therapeutic development including revised molecular docking strategies based on M pro inhibition.

59 BASIC BIOLOGICAL SCIENCES↗

Exploring electron-beam induced modifications of materials with machine-learning assisted high temporal resolution electron microscopy

Directed atomic fabrication using an aberration-corrected scanning transmission electron microscope (STEM) opens new pathways for atomic engineering of functional materials. In this approach, the electron beam is used to actively alter the atomic structure through electron beam induced irradiation processes. One of the impediments that has limited widespread use thus far has been the ability to understand the fundamental mechanisms of atomic transformation pathways at high spatiotemporal resolution. Here, we develop a workflow for obtaining and analyzing high-speed spiral scan STEM data, up to 100 fps, to track the atomic fabrication process during nanopore milling in monolayer MoS 2 . An automated feedback-controlled electron beam positioning system combined with deep convolution neural network (DCNN) was used to decipher fast but low signal-to-noise datasets and classify time-resolved atom positions and nature of their evolving atomic defect configurations. Through this automated decoding, the initial atomic disordering and reordering processes leading to nanopore formation was able to be studied across various timescales. Using these experimental workflows a greater degree of speed and information can be extracted from small datasets without compromising spatial resolution. This approach can be adapted to other 2D materials systems to gain further insights into the defect formation necessary to inform future automated fabrication techniques utilizing the STEM electron beam.

36 MATERIALS SCIENCE↗

Mapping structural heterogeneity at the nanoscale with scanning nano-structure electron microscopy (SNEM)

Here, in this work, we explore the use of scanning electron diffraction (also known as 4D-STEM) coupled with electron atomic pair distribution function analysis (ePDF) to understand the local order (structure and chemistry) as a function of position in a complex multicomponent system, a hot rolled, Ni-encapsulated, Zr 65 Cu 17.5 Ni 10 Al 7.5 bulk metallic glass (BMG), with a spatial resolution of 3 nm. We show that it is possible to gain insight into the chemistry and chemical clustering/ordering tendency in different regions of the sample, including in the vicinity of nano-scale crystallites that are identified from virtual dark field images and in heavily deformed regions at the edge of the BMG. In addition to simpler analysis, unsupervised machine learning was used to extract partial PDFs from the material, modeled as a quasi-binary alloy, and map them in space. These maps allowed key insights not only into the local average composition, as validated by EELS, but also a unique insight into chemical short-range ordering tendencies in different regions of the sample during formation. The experiments are straightforward and rapid and, unlike spectroscopic measurements, don’t require energy filters on the instrument. We spatially map different quantities of interest (QoI’s), defined as scalars that can be computed directly from positions and widths of ePDF peaks or parameters refined from fits to the patterns. We developed a flexible and rapid data reduction and analysis software framework that allows experimenters to rapidly explore images of the sample on the basis of different QoI’s. The power and flexibility of this approach are explored and described in detail. Because of the fact that we are getting spatially resolved images of the nanoscale structure obtained from ePDFs we call this approach scanning nano-structure electron microscopy (SNEM), and we believe that it will be powerful and useful extension of current 4D-STEM methods.

36 MATERIALS SCIENCE↗

Can fractal dimensions objectivize gastropod shell morphometrics? A case study from Lake Lugu (SW China)

1. Morphometrics are fundamental for the analysis of size and shape in fossils, particularly because soft parts or DNA are rarely preserved and hard parts such as shells are commonly the only source of information. Geometric morphometrics, that is, landmark analysis, is well established for the description of shape but it exhibits a couple of shortcomings resulting from subjective choices during landmarking (number and position of landmarks) and from difficulties in resolving shape at the level of micro-sculpture. 2. With the aid of high-resolution 3D scanning technology and analyses of fractal dimensions, we test whether such shortcomings of linear and landmark morphometrics can be overcome. As a model group, we selected a clade of modern viviparid gastropods from Lake Lugu, with shells that show a high degree of sculptural variation. Linear and landmark analyses were applied to the same shells in order to establish the fractal dimensions. 3. The genetic diversity of the gastropod clade was assessed. The genetic results suggest that the gastropod clade represents a single species. The results of all morphometric methods applied are in line with the genetic results, which is that no specific morphotype could be delimited. Apart from this overall agreement, landmark and fractal dimension analyses do not correspond to each other but represent data sets with different information. Generally, the fractal dimension values quantify the roughness of the shell surface, the resolution of the 3D scans determining the level. In our approach, we captured the micro-sculpture but not the first-order sculptural elements, which explains that fractal dimension and landmark data are not in phase. 4. We can show that analyzing fractal dimensions of gastropod shells opens a window to more detailed information that can be considered in evolutionary and ecological contexts. We propose that using low-resolution 3D scans may successfully substitute landmark analyses because it overcomes the subjective landmarking. Analyses of 3D scans with higher resolution than used in this study will provide surface roughness information at the mineralogical level. We suggest that fractal dimension analyses of a combination of differently resolved 3D models will significantly improve the quality of shell morphometrics.

54 ENVIRONMENTAL SCIENCES↗

Influence of ion-pairing reagents on the separation of intact glycoproteins using hydrophilic-interaction liquid chromatography - high-resolution mass spectrometry

Hydrophilic-interaction liquid chromatography (HILIC) of intact proteins offers high-resolution separations of glycoforms of glycoproteins differing in the number of (neutral) glycans. However, to obtain efficient separations it is essential that the positively charged sites of the proteins are shielded by acidic (negative) ion-pair reagents (IPRs), so as to enhance the contribution of the hydroxyl groups of the (neutral) sugars in the glycoprotein. Here, we studied the influence of various IPRs that differ in physico-chemical properties, such as hydrophobicity and acidity, on the capillary-scale HILIC separation of intact (glyco)proteins. We evaluated the use of fluoroacetic acid (MFA), difluoroacetic acid (DFA), trifluoroacetic acid (TFA), and heptafluorobutyric acid (HFBA) as diluents for sample preparation, as solvents for sample loading on a reversed-phase trap prior to the HILIC separation, and as mobile-phase components for HILIC and HILIC-MS. To reduce the contribution of ion-exchange interaction with the (silica-based) stationary phase, we used an acrylamide-based monolithic column. We studied the influence of the different IPRs on each step of the separation of a mixture of proteins of different size and hydrophilicity and on the separation of the five glycoforms of ribonuclease B. The content of IPR in the sample was shown not to affect the separation and the MS detection. However, a low content of TFA and DFA in the mobile phase is favourable, as it reduces adduct formation and leads to higher signal intensity. The optimized HILIC conditions successfully resolved nine major glycoforms groups of a ~40 kDa glycoprotein horseradish peroxidase (HRP), as an example of a complex glycoprotein.

59 BASIC BIOLOGICAL SCIENCES↗

Atom Identification in Bilayer Moiré Materials with Gomb-Net

Moiré patterns in van der Waals bilayer materials complicate the analysis of atomic-resolution images, hindering the atomic-scale insight typically attainable with scanning transmission electron microscopy. Here, we report a method to detect the positions and identities of atoms in each of the individual layers that compose twisted bilayer heterostructures. We developed a deep learning model, Gomb-Net, which identifies the coordinates and atomic species in each layer, deconvoluting the moiré pattern. This enables layer-specific mapping of atomic positions and dopant distributions, unlike other commonly used segmentation models which struggle with moiré-induced complexity. Using this approach, we explored the Se atom substitutional site distribution in a twisted fractional Janus WS 2 -WS 2(1–x) Se 2x heterostructure and found that layer-specific implantation sites are unaffected by the moiré pattern’s local energetic or electronic modulation. In conclusion, this advancement enables atom identification within material regimes where it was not possible before, opening new insights into previously inaccessible material physics.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

Design of a Thomson scattering diagnostic for the SMall Aspect Ratio Tokamak (SMART)

Here, we describe the design of a Thomson scattering (TS) diagnostic to be used on the SMall Aspect Ratio Tokamak (SMART). SMART is a spherical tokamak being commissioned in Spain that aims to explore positive triangularity and negative triangularity plasma scenarios at a low aspect ratio. The SMART TS diagnostic is designed to operate at high spatial resolution, 6 mm scattering length in the low-field side and 9 mm in the high-field side regions, and a wide dynamic range, electron temperature from 1 eV to 1 keV and density from 5 x 10 18 m -3 to 1 x 10 20 m -3 ⁠, to resolve large gradients formed at the plasma edge and in the scrape-off layer (SOL) under different triangularities and low aspect ratios. A 2 J @1064 nm laser will be used that is capable of operating in the burst mode at 1, 2, and 4 kHz to investigate fast phenomena and at 30 Hz to study 1 s (or more) long discharges. The scattered light will be collected over an angular range of 60° – 120° from 28 spatial points in the midplane covering the entire plasma width and the outer midplane SOL. Each scattering signal will be spectrally resolved on five wavelength channels of a polychromator to obtain the electron temperature measurement. We will also present a method to monitor in situ laser alignment in the core during calibrations and plasma operations.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗

A search for the lenses in the Herschel Bright Sources (HerBS) sample

ABSTRACT Verifying that sub-mm galaxies are gravitationally lensed requires time-expensive observations with oversubscribed high-resolution observatories. Here, we aim to strengthen the evidence of gravitational lensing within the Herschel Bright Sources (HerBS) by cross-comparing their positions to optical (SDSS) and near-infrared (VIKING) surveys, in order to search for the foreground lensing galaxy candidates. Resolved observations of the brightest HerBS sources have already shown that most are lensed, and a galaxy evolution model predicts that ∼76 per cent of the total HerBS sources are lensed, although with the SDSS survey we are only able to identify the likely foreground lenses for 25 per cent of the sources. With the near-infrared VIKING survey, however, we are able to identify the likely foreground lenses for 57 per cent of the sources, and we estimate that 82 per cent of the HerBS sources have lenses on the VIKING images even if we cannot identify the lens in every case. We find that the angular offsets between lens and Herschel source are larger than that expected if the lensing is done by individual galaxies. We also find that the fraction of HerBS sources that are lensed falls with decreasing 500-micron flux density, which is expected from the galaxy evolution model. Finally, we apply our statistical VIKING cross-identification to the entire Herschel-ATLAS catalogue, where we also find that the number of lensed sources falls with decreasing 500-micron flux density.

Bakx, Tom J. L. C.↗

Improving CdMnTe Detector Performance by Adding 2% of Selenium

About 2% of selenium was added to CdMnTe (CMT) to reveal the role of selenium in CMT material used as a gamma-ray detector. The average etch-pit density (EPD) in a typical 2-in CdMnTeSe (CMTS) wafer was measured to be about 8.3×10 3 cm –2 except for the edge regions, and the radial nonuniformity of the bandgap along the wafer was about 2-meV. The typical mobility-lifetime product of electrons and holes was evaluated to be 3.06×10 –3 cm 2 /V and 3.1×10 –4 cm 2 /V, respectively. The energy resolution of the 122-keV photo-peak for a Co-57 radioactive source with a planar CMTS detector was 5.2%, demonstrating a positive effect associated with adding dilute amounts of Se to CMT. Finally, the enhanced detector performance in a planar CMTS detector is attributed to an improved value of hole mobility-lifetime product in CMTS, which mitigates the adverse hole tailing effect found in CdZnTe (CZT) and CMT detectors.

47 OTHER INSTRUMENTATION↗

Structures of SARS-CoV-2 N7-methyltransferase with DOT1L and PRMT7 inhibitors provide a platform for new antivirals

The RNA N7-methyltransferase (MTase) activity of SARS-CoV-2’s nsp14 protein is essential for viral replication and is a target for the development of new antivirals. Nsp14 uses S-adenosyl methionine (SAM) as the methyl donor to cap the 5’ end of the SARS-CoV-2 mRNA and generates S-adenosyl homocysteine (SAH) as the reaction byproduct. Due to the central role of histone MTases in cancer, many SAM/SAH analogs with properties of cell permeability have recently been developed for the inhibition of these MTases. We have succeeded in identifying two such compounds (SGC0946 and SGC8158) that display significant antiviral activity and bind to the SARS-CoV-2 nsp14 N7-MTase core. Unexpectedly, crystal structures of SGC0946 and SGC8158 with the SARS-CoV-2 nsp14 N7-MTase core identify them as bi-substrate inhibitors of the viral MTase, co-occupying both the SAM and RNA binding sites; positing novel features that can be derivatized for increased potency and selectivity for SARS-CoV-2 nsp14. Taken together, the high-resolution structures and the accompanying biophysical and viral replication data provide a new avenue for developing analogs of SGC0946 and SGC8158 as antivirals.

60 APPLIED LIFE SCIENCES↗

Continuous snow depth and temperature measurements from dense network of above-ground distributed temperature profiling systems from 2021-09-23 to 2024-08-23, Seward Peninsula, Alaska

The dataset contains temperature measurements from distributed temperature profiling (DTP) systems (Dafflon et al., 2022; Wielandt et al., 2022; Wang et al., 2024a; Fiolleau et al., 2024) deployed vertically above the ground surface at a large number of locations from 2021 to 2024. The research is designed to improve understanding of the local heterogeneity in snow depth and snow thermal insulation dynamics, as well as their interactions in a discontinuous permafrost region (Wang et al., 2025). The DTP systems were deployed at 96 locations in a watershed along the Nome-Teller road at mile marker 27 (T27) and at 54 locations on a hillslope along the Kougarok road at mile marker 64 (K64) in the Seward Peninsula, Alaska. The probe location information is stored in Probe_locations_T27.csv and Probe_locations_K64.csv. Temperature measurements were recorded at 15-minute intervals using high-precision digital sensors (accuracy: ±0.1°C, resolution: 0.0078°C). The temperature probes, either 1.4 m or 1.6 m long, contain sensors spaced every 5 cm or 10 cm along their length. The temperature data are stored in compressed files following the format: DTP_snow_air_temperature_(site)_(start)_(end).zip, where site is either T27 or K64, and start and end represent the time series period. Within each ZIP file, individual CSV files are named by probe ID and contain temperature records at different heights above the ground surface.This dataset also includes derived snow depth time series over three snow seasons, estimated from temperature measurements. Snow depth was estimated by identifying the consecutive sensor pair that exhibited the largest drop in high-frequency temperature fluctuations (detailed in the methods). These data are stored in: Snow_depths_flags_(site)_(start)_(end).csv, which includes snow depth time series and corresponding quality flags (defined in the methods) from different probes. Additionally, the dataset includes derived metrics and supporting measurements at selected locations over two snow seasons, contributing to the manuscript of Wang et al., 2025. These locations were chosen based on the availability of high-quality snow depth time series during both seasons. The additional data include: (1) Air temperature proxies measured from the top sensors on the pole when they were not buried by snow, stored in Air_temperature_proxies_(site)_(start)_(end).csv (2) Ground interface temperature, recorded at 3 cm above the ground, stored in Ground_interface_temperature_(site)_(start)_(end).csv (3) Site characteristics, including vegetation height, elevation, and the topographic position index (TPI) within a 50 m radius, stored in Selected_probe_locations_gps_vegheight_tpi_elevation_(site).csv. These metrics were derived from 1 m resolution summer LiDAR-based digital elevation models and digital surface models from Singhania et al., 2023, DOI:10.5440/1832016. Metadata files include data descriptions (_dd.csv) for tabular data. All included files are listed and described in xxxx_flmd.csv.This dataset is an updated version of a previous archive (Wang et al., 2024b, DOI: 10.15485/2475020), incorporating multiple seasons and improved snow depth estimation. Please note that due to large amount of information present in this dataset, many specificities associated with the acquisition of snow temperature, air temperature proxy and estimation of snow depth, and the future archiving of additional datasets on the soil temperature, thaw depth and soil characteristics at these locations, the author would welcome being contacted by people planning to use this dataset.The Next-Generation Ecosystem Experiments: Arctic (NGEE Arctic), was a research effort to reduce uncertainty in Earth System Models by developing a predictive understanding of carbon-rich Arctic ecosystems and feedbacks to climate. NGEE Arctic was supported by the Department of Energy's Office of Biological and Environmental Research.The NGEE Arctic project had two field research sites: 1) located within the Arctic polygonal tundra coastal region on the Barrow Environmental Observatory (BEO) and the North Slope near Utqiagvik (Barrow), Alaska and 2) multiple areas on the discontinuous permafrost region of the Seward Peninsula north of Nome, Alaska.Through observations, experiments, and synthesis with existing datasets, NGEE Arctic provided an enhanced knowledge base for multi-scale modeling and contributed to improved process representation at global pan-Arctic scales within the Department of Energy's Earth system Model (the Energy Exascale Earth System Model, or E3SM), and specifically within the E3SM Land Model component (ELM).

54 ENVIRONMENTAL SCIENCES↗