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At least 307 records · Page 17

Dioctanoylglycerol stimulates accumulation of [methyl-14C]choline and its incorporation into acetylcholine and phosphatidylcholine in a human cholinergic neuroblastoma cell line

Dioctanoylglycerol, a synthetic diacylglycerol, stimulated [14C]choline uptake in cultured human neuroblastoma (LA-N-2) cells. As this effect has not, to our knowledge, been reported before, it was of interest to characterize it in more detail. In the presence of 500 microM dioctanoylglycerol the levels of [14C]choline attained during a 2 hour labeling period were elevated by 78 +/- 12%, while [14C]acetylcholine and long fatty acyl chain [14C]phosphatidylcholine levels increased by 26 +/- 2% and 19 +/- 5%, respectively (mean +/- S.E.M.). Total (long chain plus dioctanoyl-) [14C]phosphatidylcholine was increased by 198 +/- 33%. Kinetic analysis showed that dioctanoylglycerol reduced the apparent Km for choline uptake to 56 +/- 9% of control (n = 4). The Vmax was not significantly altered. The stimulation of [14C]choline accumulation by dioctanoylglycerol was not dependent on protein kinase C activation; the effect was not mimicked by phorbol ester or by 1-oleoyl-2-acetylglycerol, and was not inhibited by the protein kinase C inhibitors H-7 or staurosporine, or by prolonged pretreatment with phorbol 12-myristate 13-acetate. The effect of dioctanoylglycerol was slightly (but not significantly) reduced by EGTA and strongly inhibited by the cell-permeant calcium chelator bis(o-aminophenoxy)-ethane-N,N,N',N'-tetraacetic acid, tetra(acetoxymethyl)ester. Although these results implicate elevated intracellular calcium in the response, dioctanoylglycerol did not increase phosphatidylinositol hydrolysis in LA-N-2 cells, and its effect was not inhibited by the diacylglycerol kinase inhibitor R 59 022 (which blocks the conversion of diacylglycerol to phosphatidic acid, a known stimulator of phosphatidylinositol hydrolysis).(ABSTRACT TRUNCATED AT 250 WORDS).

Non-NASA Center↗

The Chemistry of Early Self-replicating Systems

The NASA Specialized Center of Research and Training in Exobiology (NSCORT/Exobiology) is a program within the University of California, San Diego, California Space Institute (Dr. Wolfgang Berger, Director). It has been funded by two 5-year Federal Demonstration Project Grants from NASA; and currently (February 1, 2003-December 31,2004) received supplemental funding to support our research completion of the NAG5-4546 Final Report for past 5 years, seminars, public lectures, and support for program administrative office. The program's specific aims have been: 1. The support and training of Postdoctoral, Graduate, and Undergraduate Fellows in Exobiology. 2. The support of research by the Principal Investigators and Fellows in the field of Exobiology. 3. Outreach programs emphasizing the dissemination and exchange of information concerning Exobiology within the scientific community, primary, secondary and college students, and the general public. 4. Public Lectures, Discussion S e m h q Seminars and Fellows Journals Club. 5. Host of the 2003 Public Lectures "Celebrating 50 Years of Prebiotic Chemistry" held at the University of California, San Diego in La Jolla, California on June 10,2003. 6. Host of the 1999 meeting of the International Society for the Study of the Origin of Life (ISSOL) held at the University of California, San Diego in La Jolla, California, from Sunday, July 11 through Friday, July 16,1999.

Bada, Jeffrey L.↗

Single Phase Passive Rectification Versus Active Rectification Applied to High Power Stirling Engines

Stirling engine converters are being considered as potential candidates for high power energy conversion systems required by future NASA explorations missions. These types of engines typically contain two major moving parts, the displacer and the piston, in which a linear alternator is attached to the piston to produce a single phase sinusoidal waveform at a specific electric frequency. Since all Stirling engines perform at low electrical frequencies (less or equal to 100 Hz), space explorations missions that will employ these engines will be required to use DC power management and distribution (PMAD) system instead of an AC PMAD system to save on space and weight. Therefore, to supply such DC power an AC to DC converter is connected to the Stirling engine. There are two types of AC to DC converters that can be employed, a passive full bridge diode rectifier and an active switching full bridge rectifier. Due to the inherent line inductance of the Stirling Engine-Linear Alternator (SE-LA), their sinusoidal voltage and current will be phase shifted producing a power factor below 1. In order to keep power the factor close to unity, both AC to DC converters topologies will implement power factor correction. This paper discusses these power factor correction methods as well as their impact on overall mass for exploration applications. Simulation results on both AC to DC converters topologies with power factor correction as a function of output power and SE-LA line inductance impedance are presented and compared.

Santiago, Walter↗

ECMWF and ERS-1 Surface Winds Over the Arabian Sea During July 1995

The European Center for Medium-Range Weather Forecasts (ECMWF) and Institut Francais Pour la Recherche et 1'Exploitation de la Mer (IFREMER) European Remote-Sensing Satellite (ERS-1), named IFR2, surface wind velocity data products are compared during July 1995 over the Arabian Sea.

Remote-Sensing Satellite ERS-1 Arabian Sea surface↗

Usefulness of AIRS-Derived OLR, Temperature, Water Vapor and Cloudiness Anomaly Trends for GCM Validation

Mainly due to their global nature, satellite observations can provide a very useful basis for GCM validations. In particular, satellite sounders such as AIRS provide 3-D spatial information (most useful for GCMs), so the question arises: can we use AIRS datasets for climate variability assessments? We show that the recent (September 2002 February 2010) CERES-observed negative trend in OLR of approx.-0.1 W/sq m/yr averaged over the globe is found in the AIRS OLR data as well. Most importantly, even minute details (down to 1 x 1 degree GCM-scale resolution) of spatial and temporal anomalies and trends of OLR as observed by CERES and computed based on AIRS-retrieved surface and atmospheric geophysical parameters over this time period are essentially the same. The correspondence can be seen even in the very large spatial variations of these trends with local values ranging from -2.6 W/sq m/yr to +3.0 W/sq m/yr in the tropics, for example. This essentially perfect agreement of OLR anomalies and trends derived from observations by two different instruments, in totally independent and different manners, implies that both sets of results must be highly accurate, and indirectly validates the anomalies and trends of other AIRS derived products as well. These products show that global and regional anomalies and trends of OLR, water vapor and cloud cover over the last 7+ years are strongly influenced by EI-Nino-La Nina cycles . We have created climate parameter anomaly datasets using AIRS retrievals which can be compared directly with coupled GCM climate variability assessments. Moreover, interrelationships of these anomalies and trends should also be similar between the observed and GCM-generated datasets, and, in cases of discrepancies, GCM parameterizations could be improved based on the relationships observed in the data. First, we assess spatial "trends" of variability of climatic parameter anomalies [since anomalies relative to the seasonal cycle are good proxies of climate variability] at the common 1x1 degree GCM grid-scale by creating spatial anomaly "trends" based on the first 7+ years of AIRS Version 5 Leve13 data. We suggest that modelers should compare these with their (coupled) GCM's performance covering the same period. We evaluate temporal variability and interrelations of climatic anomalies on global to regional e.g., deep Tropical Hovmoller diagrams, El-Nino-related variability scales, and show the effects of El-Nino-La Nina activity on tropical anomalies and trends of water vapor cloud cover and OLR. For GCMs to be trusted highly for long-term climate change predictions, they should be able to reproduce findings similar to these. In summary, the AIRS-based climate variability analyses provide high quality, informative and physically plausible interrelationships among OLR, temperature, humidity and cloud cover both on the spatial and temporal scales. GCM validations can use these results even directly, e. g., by creating 1x1 degree trendmaps for the same period in coupled climate simulations.

Molnar, Gyula I.↗

NASA Applied Sciences' DEVELOP National Program: Summer 2010 Florida Agriculture

The main agricultural areas in South Florida are located within the fertile land surrounding Lake Okeechobee. The Atlantic Watershed monthly rainfall anomalies showed a weak but statistically significant correlation to the Oceanic Nino Index (ONI). No other watershed s anomalies showed significant correlations with ONI or the Southern Oscillation Index (SOI). During La Nina months, less sea breeze days and more disturbed days were found to occur compared to El Nino and neutral months. The increase in disturbed days can likely by attributed to the synoptic pattern during La Nina, which is known to be favorable for tropical systems to follow paths that affect South Florida. Overall, neither sea breeze rainfall patterns nor total rainfall patterns in South Florida s main agricultural areas were found to be strongly influenced by the El Nino Southern Oscillation during our study time.

Cooley, Zachary C.↗

Recent Spatial and Temporal Anomalies and Trends of OLR as Observed by CERES and Computed Based on AIRS Retrievals

We show that a recent CERES-observed negative trend in OLR of approx.-0.1 W/sq m/yr averaged over the globe, for the time period of September 2002 through February 2010 used in this study, is found in the AIRS OLR data as well. Most importantly, even minute details (down to 1 x 1 Degree GCM-scale resolution) of spatial and temporal anomalies and trends of OLR as observed by CERES and computed based on AIRS-retrieved surface and atmospheric geophysical parameters over this time period are essentially the same. We see this correspondence even in the very large spatial variations of these trends with local values ranging from -2.6 W/sq m/yr to +3.0 W/sq m/yr in the tropics. This essentially perfect agreement of OLR anomalies and even local trends derived from observations by two different instruments, in totally independent and different manners, implies that both sets of results must be highly accurate; and indirectly validates the anomalies and trends of other AIRS derived products as well. These products show that global and regional anomalies and trends of OLR, water vapor and cloud cover over the last 7+ years are strongly influenced by El-Nino-La Nina cycles . We use the anomalies and trends of AIRS derived products to explain why the global OLR has a large negative trend over this time period; Global and tropical OLR began to decrease significantly at the onset of a strong La Nina in mid-2007. AIRS products show that cloudiness and mid-tropospheric water vapor began to increase in the tropics at roughly the same time, especially in the region 5degN - 20degS latitude extending eastward from 150degW to 30degE longitude, with a corresponding very large drop in OLR in this region. Late 2009 is characterized by a strong El-Nino, with a corresponding change in sign of observed tropical water vapor, cloud cover, and OLR anomalies. If one excludes the area 5degN - 20degS, 150degW - 30degE from the statistics, area mean OLR trends over the rest of the globe are substantially reduced over the time period under study.

Molnar, Gyula↗

AIRS Water Vapor and Cloud Products Validate and Explain Recent Short Term Decreases in Global and Tropical OLR as Observed by CERES

A strong equatorial SST cooling occurred from 160E westward to 120W during the period of September 2002 through August 2010, surrounded by a weaker warming ring to the west. This is the result of a transition from a strong El Nino in late 2002 to a strong La Nina in 2008. Late 2009 is characterized by the beginning of another El Nino. Average rates of change (ARC's) in 500mb specific humidity and cloud cover are in phase with those in the Sea surface temperature (SST). In the El Nino and surrounding region causing outgoing longwave radiation (OLR), to decrease significantly near the dateline and increase in the vicinity of Indonesia. Tropical OLR ARC's in these two areas cancel each other to first order. The negative zonal mean tropical OLR ARC from a drop in equatorial OLR in region 1 from 140W to 40E. This results from increasing water vapor and cloud cover in this area during La Nina with the reverse holding during El Nino.

Susskind, Joel↗

Suzaku Observations of the HMXB 1A 1118-61

We present broad band analysis of the Be/X-ray transient lA 1118-61 by Suzaku at the peak of its 3rd observed outburst in January 2009 and 2 weeks later when the source flux had decayed by an order of magnitude. The continuum was modeled with a cutoffpl model as well as a compTT model, with both cases requiring an additional black body component at lower energies, We confirm the detection of a cyclotron line at approx. 55 keV and discuss the possibility of a first harmonic at approx. 110 keV. Pulse profile comparisons show a change in the profile structure at lower energies, an indication for possible changes in the accretion geometry, Phase resolved spectroscopy in the outburst data show a change in the continuum throughout the pulse period. The decrease in the CRSF centroid energy also indicates that the viewing angle on the accretion column is changing throughout the pulse period. Subject headings: X-rays: stars - X-rays: binaries - stars: pulsars: individual (lA 1118-61) - stars: magnetic fields

Suchy, Slawomir↗

The Arctic Vortex in March 2011: A Dynamical Perspective

Despite the record ozone loss observed in March 2011, dynamical conditions in the Arctic stratosphere were unusual but not unprecedented. Weak planetary wave driving in February preceded cold anomalies in t he polar lower stratosphere in March and a relatively late breakup of the Arctic vortex in April. La Nina conditions and the westerly phas e of the quasi-biennial oscillation (QBO) were observed in March 201 1. Though these conditions are generally associated with a stronger vortex in mid-winter, the respective cold anomalies do not persist t hrough March. Therefore, the La Nina and QBO-westerly conditions cannot explain the observed cold anomalies in March 2011. In contrast, po sitive sea surface temperature anomalies in the North Pacific may ha ve contributed to the unusually weak tropospheric wave driving and s trong Arctic vortex in late winter 2011.

Hurwitz, Margaret M.↗

Diagnosing the Sensitivity of Local Land-Atmosphere Coupling via the Soil Moisture-Boundary Layer Interaction

The inherent coupled nature of earth s energy and water cycles places significant importance on the proper representation and diagnosis of land atmosphere (LA) interactions in hydrometeorological prediction models. However, the precise nature of the soil moisture precipitation relationship at the local scale is largely determined by a series of nonlinear processes and feedbacks that are difficult to quantify. To quantify the strength of the local LA coupling (LoCo), this process chain must be considered both in full and as individual components through their relationships and sensitivities. To address this, recent modeling and diagnostic studies have been extended to 1) quantify the processes governing LoCo utilizing the thermodynamic properties of mixing diagrams, and 2) diagnose the sensitivity of coupled systems, including clouds and moist processes, to perturbations in soil moisture. This work employs NASA s Land Information System (LIS) coupled to the Weather Research and Forecasting (WRF) mesoscale model and simulations performed over the U.S. Southern Great Plains. The behavior of different planetary boundary layers (PBL) and land surface scheme couplings in LIS WRF are examined in the context of the evolution of thermodynamic quantities that link the surface soil moisture condition to the PBL regime, clouds, and precipitation. Specifically, the tendency toward saturation in the PBL is quantified by the lifting condensation level (LCL) deficit and addressed as a function of time and space. The sensitivity of the LCL deficit to the soil moisture condition is indicative of the strength of LoCo, where both positive and negative feedbacks can be identified. Overall, this methodology can be applied to any model or observations and is a crucial step toward improved evaluation and quantification of LoCo within models, particularly given the advent of next-generation satellite measurements of PBL and land surface properties along with advances in data assimilation schemes.

Santanello, Joseph A., Jr.↗

Updates of the KArLE Experiment: New Libs Calibration Under High Vacuum for the Quantification of Potassium in Basalt for In Situ Geochronology

In planetary exploration, in situ absolute geochronology is one of the main important measurements that needs to be accomplished. Until now, on Mars, the age of the surface is only determined by crater density counting, which gives relative ages. These ages can have a lot of uncertainty as they depend on many parameters. More than that, the curves must be ties to absolute ages. Thus far, only the lost lander Beagle 2 was designed to conduct absolute geochronology measurements, though some recent attempts using MSL Curiosity show that this investigation is feasible and should be strongly encouraged for future flight. Experimental: The Potassium (K)-Argon Laser Experiment (KArLE) is being developed at MSFC through the NASA Planetary Instrument Definition and Development Program (PIDDP). The goal of this experiment is to provide in situ geochronology based on the K-Ar method. A laser ablates a rock under high vacuum, creating a plasma which is sensed by an optical spectrometer to do Laser Induced Breakdown Spectroscopy (LIBS). The ablated material frees gases, including radiogenic 40Ar,which is measured by a mass spectrometer (MS). As the potassium is a content and the 40Ar is a quantity, the ablated mass needed in order to relate them. The mass is given by the product of the ablated volume by the density of this material. So we determine the mineralogy of the ablated material with the LIBS spectra and images and calculate its density. The volume of the pit is measured by using microscopy. LIBS measurement of K under high vacuum: Three independant projects [1, 2, 3] including KArLE, are developing geochronological instruments based on this LA-LIBS-MS method. Despite several differences in their setup, all of them have validated the methods with analyses and ages. However, they all described difficulties with the LIBS measurements of K [3,4]. At ambient pressure, the quantification of K by LIBS on geological materials can be accurate [5]. However the protocol of the LA-LIBS-MS experiment required hundreds of shots under high vacuum in order to free enough 40Ar* to be measured by the QMS. This long duration of ablation may induces significant changes in the LIBS spectra. The pressure may increases by orders of magnitudewithin the chamber and the laser pit geometry can change the effectiveness of ablation and intensity of plasma light received. These effects introduce variation between the first and last spectra and so the quantification of K is more complex. The ablation of one crater can give, depending on the protocol of acquisition, from tens to hundreds of spectra. Protocol and results: We are in the process of further characterizing the variation introduced into LIBS spectra by the use of hundreds of laser shots, and definining a protocol that can be used to ensure accuracy and reporoducibility in the results.We are using natural rock powder standards fused in a furnace, as well as mars analog samples with known K content. We will show the result of the calibration and some new statistical approaches in order to apprehend the effects of the long time ablation on rocks under high vacuum.

Devismes, D.↗

Colombia Mi Pronostico Flood Application: Updating and Improving the Mi Pronostico Flood Web Application to Include an Assessment of Flood Risk

Colombia is a country with highly variable terrain, from the Andes Mountains to plains and coastal areas, many of these areas are prone to flooding disasters. To identify these risk areas NASA's Advanced Spaceborne Thermal Emission and Reflection Radiometer (ASTER) was used to construct a digital elevation model (DEM) for the study region. The preliminary risk assessment was applied to a pilot study area, the La Mosca River basin. Precipitation data from the National Aeronautics and Space Administration (NASA) Tropical Rainfall Measuring Mission (TRMM)'s near-real-time rainfall products as well as precipitation data from the Instituto de Hidrologia, Meteorologia y Estudios Ambientales (the Institute of Hydrology, Meteorology and Environmental Studies, IDEAM) and stations in the La Mosca River Basin were used to create rainfall distribution maps for the region. Using the precipitation data and the ASTER DEM, the web application, Mi Pronóstico, run by IDEAM, was updated to include an interactive map which currently allows users to search for a location and view the vulnerability and current weather and flooding conditions. The geospatial information was linked to an early warning system in Mi Pronóstico that can alert the public of flood warnings and identify locations of nearby shelters.

Rushley, Stephanie↗

Identification of Martian Regolith Sulfur Components in Shergottites Using Sulfur K Xanes and Fe/S Ratios

Based on isotopic anomalies in Kr and Sm, Sr-isotopes, S-isotopes, XANES results on S-speciation, Fe/S ratios in sulfide immiscible melts [5], and major element correlations with S determined in impact glasses in EET79001 Lith A & Lith B and Tissint, we have provided very strong evidence for the occurrence of a Martian regolith component in some impact melt glasses in shergottites. Using REE measurements by LA-ICP-MS in shergottite impact glasses, Barrat and co-workers have recently reported conflicting conclusions about the occurrence of Martian regolith components: (a) Positive evidence was reported for a Tissint impact melt, but (b) Negative evidence for impact melt in EET79001 and another impact melt in Tissint. Here, we address some specific issues related to sulfur speciation and their relevance to identifying Martian regolith components in impact glasses in EET79001 and Tissint using sulfur K XANES and Fe/S ratios in sulfide immiscible melts. XANES and FE-SEM measurements in approx. 5 micron size individual sulfur blebs in EET79001 and Tissint glasses are carried out by us using sub-micron size beams, whereas Barrat and coworkers used approx. 90 micron size laser spots for LA- ICP-MS to determine REE abundances in bulk samples of the impact melt glasses. We contend that Martian regolith components in some shergottite impact glasses are present locally, and that studying impact melts in various shergottites can give evidence both for and against regolith components because of sample heterogeneity.

Sutton, S. R.↗

Surface Ozone Variability and Trends over the South African Highveld from 1990 to 2007

Surface ozone is a secondary air pollutant formed from reactions between nitrogen oxides (NOx = NO + NO2) and volatile organic compounds in the presence of sunlight. In this work we examine effects of the climate pattern known as the El Niño-Southern Oscillation (ENSO) and NOx variability on surface ozone from 1990 to 2007 over the South African Highveld, a heavily populated region in South Africa with numerous industrial facilities. Over summer and autumn (December-May) on the Highveld, El Niño, as signified by positive sea surface temperature (SST) anomalies over the central Pacific Ocean, is typically associated with drier and warmer than normal conditions favoring ozone formation. Conversely, La Niña, or negative SST anomalies over the central Pacific Ocean, is typically associated with cloudier and above normal rainfall conditions, hindering ozone production. We use a generalized regression model to identify any linear dependence that the Highveld ozone, measured at five air quality monitoring stations, may have on ENSO and NOx. Our results indicate that four out of the five stations exhibit a statistically significant sensitivity to ENSO at some point over the December-May period where El Niño amplifies ozone formation and La Niña reduces ozone formation. Three out of the five stations reveal statistically significant sensitivity to NOx variability, primarily in winter and spring. Accounting for ENSO and NOx effects throughout the study period of 18 years, two stations exhibit statistically significant negative ozone trends in spring, one station displays a statistically significant positive trend in August, and two stations show no statistically significant change in surface ozone.

NOx↗

Mapping Successional Stages in a Wet Tropical Forest Using Landsat ETM+ and Forest Inventory Data

In this study, we test whether an existing classification technique based on the integration of Landsat ETM+ and forest inventory data enables detailed characterization of successional stages in a wet tropical forest site. The specific objectives were: (1) to map forest age classes across the La Selva Biological Station in Costa Rica; and (2) to quantify uncertainties in the proposed approach in relation to field data and existing vegetation maps. Although significant relationships between vegetation height entropy (a surrogate for forest age) and ETM+ data were detected, the classification scheme tested in this study was not suitable for characterizing spatial variation in age at La Selva, as evidenced by the error matrix and the low Kappa coefficient (12.9%). Factors affecting the performance of the classification at this particular study site include the smooth transition in vegetation structure between intermediate and advanced successional stages, and the low sensitivity of NDVI to variations in vertical structure at high biomass levels.

forest structure↗

Combined Effect of El Nino Southern Oscillation and Atlantic Multidecadal Oscillation on Lake Chad Level Variability Region

In this study, the combined effect of the Atlantic Multidecadal Oscillation (AMO) and El Niño Southern Oscillation (ENSO) on the Lake Chad (LC) level variability is explored. Our results show that the lake level at the Bol monitoring station has a statistically significant correlation with precipitation (R2 = 0.6, at the 99.5% confidence level). The period between the late 1960s and early 1970s marked a turning point in the response of the regional rainfall to climatic drivers, thereby severely affecting the LC level. Our results also suggest that the negative impact of the cold phase of AMO on Sahel precipitation masks and supersedes the positive effect of La Niña in the early the 1970s. The drop in the size of LC level from 282.5 m in the early 1960s to about 278.1 m in 1983/1984 was the largest to occur within the period of study (1900-2010) and coincides with the combined cold phase of AMO and strong El Niño phase of ENSO. Further analyses show that the current warm phase of AMO and increasing La Niña episodes appear to be playing a major role in the increased precipitation in the Sahel region. The LC level is responding to this increase in precipitation by a gradual recovery, though it is still below the levels of the 1960s. This understanding of the AMO-ENSO-rainfall-LC level association will help in forecasting the impacts of similar combined episodes in the future. These findings also have implications for long-term water resources management in the LC region.

ENSO↗

A Pan-Tropical Cascade of Fire Driven by El Nino/Southern Oscillation

The El Nino/Southern Oscillation (ENSO) has a pronounced influence on year-to-year variations in climate. The response of fires to this forcing is complex and has not been evaluated systematically across different continents. Here we use satellite data to create a climatology of burned-area and fire emissions responses, drawing on six El Nino and six La Nina events during 1997-2016. On average, reductions in precipitation and terrestrial water storage increased fire emissions in pan-tropical forests by 133% during and following El Nino as compared with La Nina. Fires peaked in equatorial Asia early in the ENSO cycle when El Nino was strengthening (Aug-Oct), before moving to southeast Asia and northern South America (Jan-Apr), Central America (Mar-May) and the southern Amazon (Jul-Oct) during the following year. Large decreases in fire occurred across northern Australia during Sep-Oct of the second year from a reduced fuel availability. Satellite observations of aerosols and carbon monoxide provided independent confirmation of the spatiotemporal evolution of fire anomalies. The predictable cascade of fire across different tropical continents described here highlights an important time delay in the Earth system's response to precipitation redistribution. These observations help to explain why the growth rate of atmospheric CO2 increases during El Nino and may contribute to improved seasonal fire forecasts.

Chen, Yang↗