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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.
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Transition from molecular diffusion to natural circulation mode air-ingress in high temperature helium loop
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Hemoglobin crystals immersed in liquid oxygen reveal diffusion channels
Highlights: • Crystal structure of Human Hemoglobin were solved after immersion in liquid oxygen. • Immersion in liquid oxygen oxidizes the heme-center revealing aquomethemoglobin. • Immersion in liquid oxygen also reveals the footprints of oxygen channels. • These channels contain medically relevant mutations that modulate oxygen affinity. Human hemoglobin (HbA) transports molecular oxygen (O{sub 2}) from the lung to tissues where the partial pressure of O{sub 2} is lower. O{sub 2} binds to HbA at the heme cofactor and is stabilized by a distal histidine (HisE7). HisE7 has been observed to occupy opened and closed conformations, and is postulated to act as a gate controlling the binding/release of O{sub 2}. However, it has been suggested that HbA also contains intraprotein oxygen channels for entrances/exits far from the heme. In this study, we developed a novel method of crystal immersion in liquid oxygen prior to X-ray data collection. In the crystals immersed in liquid oxygen, the heme center was oxidized to generate aquomethemoglobin. Increases of structural flexibility were also observed in regions that are synonymous with previously postulated oxygen channels. These regions also correspond to medically relevant mutations which affect O{sub 2} affinity. The way HbA utilizes these O{sub 2} channels could have a profound impact on understanding the relationship of HbA O{sub 2} transport within these disease conditions. Finally, the liquid oxygen immersion technique can be utilized as a new tool to crystallographically examine proteins and protein complexes which utilize O{sub 2} for enzyme catalysis or transport.
Convection-diffusion-reaction of CO2-enriched brine in porous media: A pore-scale study
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Diffusive retention of carbon and nitrogen in a microcrystalline quartz-dominated chert: Implications for reconstructing Earth's ancient atmosphere
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Effect of axial diffusion on the response of diffusion flames to axial flow perturbations
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The effects of incident electric fields on counterflow diffusion flames
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The breakdown of self-similarity in electrified counterflow diffusion flames
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Counterflow diffusion flame oscillations induced by ns pulse electric discharge waveforms
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Solubility and diffusivity of CO2 in ionic polyimides with [C(CN)3]x[oAc]1-x anion composition
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Experimental Investigation and Numerical Determination of Custom Gas Diffusion Layers on PEMFC Performance
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Wettability effects of thin titanium liquid/gas diffusion layers in proton exchange membrane electrolyzer cells
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