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At least 307 records · Page 17

Comparative Phenotyping of Two Commonly Used Chlamydomonas reinhardtii Background Strains: CC-1690 (21gr) and CC-5325 (The CLiP Mutant Library Background)

The unicellular green alga Chlamydomonas reinhardtii is an excellent model organism to investigate many essential cellular processes in photosynthetic eukaryotes. Two commonly used background strains of Chlamydomonas are CC-1690 and CC-5325. CC-1690, also called 21gr, has been used for the Chlamydomonas genome project and several transcriptome analyses. CC-5325 is the background strain for the Chlamydomonas Library Project (CLiP). Photosynthetic performance in CC-5325 has not been evaluated in comparison with CC-1690. Additionally, CC-5325 is often considered to be cell-wall deficient, although detailed analysis is missing. The circadian rhythms in CC-5325 are also unclear. To fill these knowledge gaps and facilitate the use of the CLiP mutant library for various screens, we performed phenotypic comparisons between CC-1690 and CC-5325. Our results showed that CC-5325 grew faster heterotrophically in dark and equally well in mixotrophic liquid medium as compared to CC-1690. CC-5325 had lower photosynthetic efficiency and was more heat-sensitive than CC-1690. Furthermore, CC-5325 had an intact cell wall which had comparable integrity to that in CC-1690 but appeared to have reduced thickness. Additionally, CC-5325 could perform phototaxis, but could not maintain a sustained circadian rhythm of phototaxis as CC1690 did. Finally, in comparison to CC-1690, CC-5325 had longer cilia in the medium with acetate but slower swimming speed in the medium without nitrogen and acetate. Our results will be useful for researchers in the Chlamydomonas community to choose suitable background strains for mutant analysis and employ the CLiP mutant library for genome-wide mutant screens under appropriate conditions, especially in the areas of photosynthesis, thermotolerance, cell wall, and circadian rhythms.

59 BASIC BIOLOGICAL SCIENCES↗

Comparing the Use of High- to Low-Cost Black Carbon and Carbon Dioxide Sensors for Characterizing On-Road Diesel Truck Emissions

The exhaust plume capture method is a commonly used approach to measure pollutants emitted by in-use heavy-duty diesel trucks. Lower cost sensors, if used in place of traditional research-grade analyzers, could enable wider application of this method, including use as a monitoring tool to identify high-emitting trucks that may warrant inspection and maintenance. However, low-cost sensors have for the most part only been evaluated under ambient conditions as opposed to source-influenced environments with rapidly changing pollutant concentrations. This study compared black carbon (BC) emission factors determined using different BC and carbon dioxide (CO 2 ) sensors that range in cost from $200 to $20,000. Controlled laboratory experiments show that traditional zero and span steady-state calibration checks are not robust indicators of sensor performance when sampling short duration concentration peaks. Fleet BC emission factor distributions measured at two locations at the Port of Oakland in California with 16 BC/CO 2 sensor pairs were similar, but unique sensor pairs identified different high-emitting trucks. At one location, the low-cost PP Systems SBA-5 agreed on the classification of 90% of the high emitters identified by the LI-COR LI-7000 when both were paired with the Magee Scientific AE33. Conversely, lower cost BC sensors when paired with the LI-7000 misclassified more than 50% of high emitters when compared to the AE33/LI-7000. Confidence in emission factor quantification and high-emitter identification improves with larger integrated peak areas of CO 2 and especially BC. This work highlights that sensor evaluation should be conducted under application-specific conditions, whether that be for ambient air monitoring or source characterization.

47 OTHER INSTRUMENTATION↗

Comparing XRISM Cluster Velocity Dispersions with Predictions from Cosmological Simulations: Are Feedback Models Too Ejective?

The dynamics of the intracluster medium (ICM), the hot plasma that fills galaxy clusters, are shaped by gravity-driven cluster mergers and feedback from supermassive black holes (SMBHs) in the cluster cores. XRISM measurements of ICM velocities in several clusters offer insights into these processes. We compare XRISM measurements for nine galaxy clusters (Virgo, Perseus, Centaurus, Hydra-A, PKS 0745–19, A2029, Coma, A2319, and Ophiuchus) with predictions from three state-of-the-art cosmological simulation suites, TNG-Cluster, the Three Hundred Project GADGET-X, and GIZMO-SIMBA, that employ different models of feedback. In cool cores, XRISM reveals systematically lower velocity dispersions than the simulations predict, with all 10 measurements below the median simulated values by a factor of 1.5–1.7 on average and all falling within the bottom 10% of the predicted distributions. The observed kinetic-to-total pressure ratio is also lower, with a median value of 2.2%, compared to the predicted 5.0%–6.5% for the three simulations. Outside the cool cores and in non-cool-core (NCC) clusters, simulations show better agreement with XRISM measurements, except for the outskirts of the relaxed, cool-core cluster A2029, which exhibits an exceptionally low kinetic pressure support (<1%), with none of the simulated systems in either of the three suites reaching such low levels. The NCC Coma and A2319 exhibit dispersions at the lower end but within the simulated spread. Our comparison suggests that the three numerical models may overestimate the kinetic effects of SMBH feedback in cluster cores. Additional XRISM observations of NCC clusters will clarify if there is a systematic tension in the gravity-dominated regime as well.

galaxy clusters↗

Measurement report: A comparative analysis of an intensive incursion of fluorescing African dust particles over Puerto Rico and another over Spain

Measurements during episodes of African dust, made with two wideband integrated bioaerosol spectrometers (WIBSs), one on the northeastern coast of Puerto Rico and the other in the city of León, Spain, show unmistakable, bioaerosol-like fluorescing aerosol particles (FAPs) that can be associated with these dust episodes. The Puerto Rico event occurred during a major incursion of African dust during June 2020. The León event occurred in the late winter and spring of 2022, when widespread, elevated layers of dust inundated the Iberian Peninsula. Satellite and back-trajectory analyses confirm that dust from northern Africa was the source of the particles during both events. The WIBSs measure the size of individual particles in the range from 0.5 to 30 µm, derive a shape factor, and classify seven types of fluorescence from the FAPs. In general, it is not possible to directly determine the specific biological identity from fluorescence signatures; however, measurements of these types of bioaerosols in laboratory studies allow us to compare ambient fluorescence patterns with whole microbial cells measured under controlled conditions. Here we introduce some new metrics that offer a more quantitative approach for comparing FAP characteristics derived from particles measured under different environmental conditions. The analysis highlights the similarities and differences at the two locations and reveals differences that can be attributed to the age and history of the dust plumes, e.g., the amount of time that the air masses were in the mixed layer and the frequency of precipitation along the air mass trajectory.

54 ENVIRONMENTAL SCIENCES↗

Comparing the Performance of Julia on CPUs versus GPUs and Julia-MPI versus Fortran-MPI: a case study with MPAS-Ocean (Version 7.1)

Abstract. Some programming languages are easy to develop at the cost of slow execution, while others are fast at runtime but much more difficult to write. Julia is a programming language that aims to be the best of both worlds – a development and production language at the same time. To test Julia's utility in scientific high-performance computing (HPC), we built an unstructured-mesh shallow water model in Julia and compared it against an established Fortran-MPI ocean model, the Model for Prediction Across Scales–Ocean (MPAS-Ocean), as well as a Python shallow water code. Three versions of the Julia shallow water code were created: for single-core CPU, graphics processing unit (GPU), and Message Passing Interface (MPI) CPU clusters. Comparing identical simulations revealed that our first version of the Julia model was 13 times faster than Python using NumPy, where both used an unthreaded single-core CPU. Further Julia optimizations, including static typing and removing implicit memory allocations, provided an additional 10–20× speed-up of the single-core CPU Julia model. The GPU-accelerated Julia code was almost identical in terms of performance to the MPI parallelized code on 64 processes, an unexpected result for such different architectures. Parallelized Julia-MPI performance was identical to Fortran-MPI MPAS-Ocean for low processor counts and ranges from 2× faster to 2× slower for higher processor counts. Our experience is that Julia development is fast and convenient for prototyping but that Julia requires further investment and expertise to be competitive with compiled codes. We provide advice on Julia code optimization for HPC systems.

54 ENVIRONMENTAL SCIENCES↗

A Comparative Radiative Property Evaluation of Sintered Bauxite and AMS4003 Ceramic Particles

The radiative properties of sintered bauxite (ACCUCAST ID80) and AMS4003 particles were measured and compared to assess their performances as direct absorption and heat transfer media for particle-based concentrated solar power (CSP) plants. Reflectance measurements were performed over the spectral range 0.2–20 µm and used to calculate solar and thermal emission properties of the particles. In addition, reflectance was measured as a function of temperature up to 1000 °C for the spectral range 1–20 µm. The solar absorptance of AMS4003 was greater than that of ACCUCAST both before and after thermal cycling of the materials at 1000 °C, although thermal cycling was found to substantially decrease the solar absorptance of both materials. The thermal emittance of AMS4003 was also greater than that of ACCUCAST at all temperatures tested. Finally, the radiaitve properties measured in this study were used to estimate absorber efficiencies as a way to compare the particles in terms of their performance in a solar receiver. AMS4003 yielded greater efficiencies than ACCUCAST, suggesting its potential as a material for CSP.

14 SOLAR ENERGY↗

Medium Voltage Testbed for Comparing Advanced Power Line Sensors vs. Transformers with Electrical Grid Events

Electrical utilities have relied upon potential transformers (PTs) and current transformers (CTs) for very accurate metering and to provide reliable signals for protective relays. These devices measure phase voltages and currents and are commissioned by electrical engineers. PTs/CTs can detect and react to various electrical anomalies that could adversely affect electrical grid operations. Less expensive alternative sensing technologies offer the possibility of wider deployment, particularly in grids that employ distributed energy resources. In this work, the performance of an advanced medium-voltage sensor is compared with that of a reference PT and a CT and experimentally evaluated for different power grid scenarios on an advanced outdoor power line sensor testbed at the U.S. Department of Energy’s Oak Ridge National Laboratory. The sensor is based on a capacitive divider for voltage monitoring and a Rogowski coil with an integrator for current monitoring. This study simulated a power grid model based on a utility circuit at the Riverside EPB of Chattanooga. The simulation circuit was created with MATLAB/Simulink software and was integrated into an RT-LAB project to run with the OP4510 real-time simulator at the OPLST. During the tests, the real-time simulations were run for 40 s, and the signal to record the test event with the power meter was set at 30 s for the event-trigger circuit. The advanced outdoor power line sensor testbed has a real-time simulator that is used to generate transient scenarios (e.g., electrical faults, capacitor bank operation, and service restoration), while the analog signals are recorded by the same high-resolution power meter. The behaviors of analog signals, harmonic components, total harmonic distortion, and crest factors are assessed for this power line sensor and compared with those of the reference PT/CT because of the absence of testing standards for advanced outdoor power line sensors. The results showed that this OPLS technology responded identically to the PT and CT under all conditions.

Piesciorovsky, Emilio [ORNL]↗

Globular-cluster stars - Results of theoretical evolution and pulsation studies compared with the observations.

Survey of recently published studies on globular clusters, and comparison of stellar evolution and pulsation theory with reported observations. The theory of stellar evolution is shown to be capable of describing, in principle, the behavior of a star through all quasi-static stages. Yet, as might be expected, estimates of bulk properties obtained by comparing observations with results of pulsation and stellar atmosphere theory differ somewhat from estimates of these same properties obtained by comparing observations with results of evolution theory. A description is given of how such estimates are obtained, and suggestions are offered as to where the weak points in each theory may lie.

Iben, I., Jr.↗

Comparative cut-bar thermal conductivity apparatus.

Construction details, error analysis, and typical data for a rapid comparative device for thermal conductivity measurements are presented. The apparatus consists of a comparative cut-bar arrangement contained in a bell-jar. The cylinder shaped specimen is placed between two ARMCO-iron meter bars of the same diameter. The upper bar is equipped with a heater, while the lower one is terminated in a heat sink. The temperature gradient along the bars is measured with a series of copper/constantan microthermocouples, spot welded to them. The assembly is held together by loading the bars to a desired pressure, via a pneumatic cylinder. Contact resistance at the boundaries between the specimen and the meter bars is minimized by using a Hg/In/Ga alloy film at the interface and sufficient loading force. The gradients determined for the meter bars then may be extrapolated to the boundaries yielding the temperature drop along the specimen. Using two different length specimens, the magnitude of the temperature drop across the interface can be computed, or the true gradient along the specimen can be obtained from the difference in overall temperature drops and lengths.

Gaal, P. S.↗

Comparative evaluation of solar, fission, fusion, and fossil energy resources, part 3

The role of nuclear fission reactors in becoming an important power source in the world is discussed. The supply of fissile nuclear fuel will be severely depleted by the year 2000. With breeder reactors the world supply of uranium could last thousands of years. However, breeder reactors have problems of a large radioactive inventory and an accident potential which could present an unacceptable hazard. Although breeder reactors afford a possible solution to the energy shortage, their ultimate role will depend on demonstrated safety and acceptable risks and environmental effects. Fusion power would also be a long range, essentially permanent, solution to the world's energy problem. Fusion appears to compare favorably with breeders in safety and environmental effects. Research comparing a controlled fusion reactor with the breeder reactor in solving our long range energy needs is discussed.

Clement, J. D.↗

Comparative Evaluation of Phase 1 Results from the Energy Conversion Alternatives Study (ECAS)

Ten advanced energy conversion systems for central-station, based-load electric power generation using coal and coal-derived fuels which were studied by NASA are presented. Various contractors were selected by competitive bidding to study these systems. A comparative evaluation is provided of the contractor results on both a system-by-system and an overall basis. Ground rules specified by NASA, such as coal specifications, fuel costs, labor costs, method of cost comparison, escalation and interest during construction, fixed charges, emission standards, and environmental conditions, are presented. Each system discussion includes the potential advantages of the system, the scope of each contractor's analysis, typical schematics of systems, comparison of cost of electricity and efficiency for each contractor, identification and reconciliation of differences, identification of future improvements, and discussion of outside comments. Considerations common to all systems, such as materials and furnaces, are also discussed. Results of selected in-house analyses are presented, in addition to contractor data. The results for all systems are then compared.

Source record↗

Geologic guide to the island of Hawaii: A field guide for comparative planetary geology

With geological data available for all inner planets except Venus, we are entering an era of true comparative planetary geology, when knowledge of the differences and similarities for classes of structures (e.g., shield volcanoes) will lead to a better understanding of general geological processes, regardless of planet. Thus, it is imperative that planetologists, particularly those involved in geological mapping and surface feature analysis for terrestrial planets, be familiar with volcanic terrain in terms of its origin, structure, and morphology. One means of gaining this experience is through field trips in volcanic terrains - hence, the Planetology Conference in Hawaii. In addition, discussions with volcanologists at the conference provide an important basis for establishing communications between the two fields that will facilitate comparative studies as more data become available.

Greeley, R.↗

An initial comparative assessment of orbital and terrestrial central power systems

Orbital solar power plants, which beam power to earth by microwave, are compared with ground-based solar and conventional baseload power plants. Candidate systems were identified for three types of plants and the selected plant designs were then compared on the basis of economic and social costs. The representative types of plant selected for the comparison are: light water nuclear reactor; turbines using low BTU gas from coal; central receiver with steam turbo-electric conversion and thermal storage; silicon photovoltaic power plant without tracking and including solar concentration and redox battery storage; and silicon photovoltaics.

Caputo, R.↗

Comparative orthostatic responses - Standing vs. head-up tilt

In both physiologic and clinical studies, standing is often loosely equated with head-up tilt (HUT) although the cardiovascular effects of these variants of orthostasis have not been formally compared, except for heart rate. The effects of HUT and standing were measured noninvasively in male volunteers satisfying identical criteria for age and normal status. In both postures, there were significant increases in heart rate, pre-ejection period, isovolumic contraction time, and the ratio of pre-ejection to ejection period, and significant decreases in ejection period and ejection time index. All of these changes were greater for standing than for tilt. Pulse transmission time changed significantly (-14%) only with tilt. We conclude that head-up tilt and standing are rather comparable but not physiologically identical forms of orthostasis.

Spodick, D. H.↗

The effect of technology advancements on the comparative advantages of electric versus chemical propulsion for a large cargo orbit transfer vehicle

Techniques for sizing electrically or chemically propelled orbit transfer vehicles and analyzing fleet requirements are used in a comparative analysis of the two concepts for various levels of traffic to geosynchronous orbit. The vehicle masses, fuel requirements, and fleet sizes are determined and translated into launch vehicle payload requirements. Technology projections beyond normal growth are made and their effect on the comparative advantages of the concepts is determined. A preliminary cost analysis indicates that although electric propulsion greatly reduces launch vehicle requirements substantial improvements in the cost and reusability of power systems must occur to make an electrically propelled vehicle competitive.

Rehder, J. J.↗

Comparative vibration environments of transportation vehicles

Measured vibration data are presented for a number of air and surface vehicles. Consideration is given to the importance of direction effects; of vehicle operating modes such as takeoff, cruise, and landing; and of measurement location on the level and frequency of the measurements. Various physical measurement units or descriptors are used to quantify and compare the data. Results suggest the range of vibration associated with a particular mode of transportation and illustrate the comparative levels in terms of each of the descriptors. Collectively, the results form a data base which may be useful in assessing the ride of existing or future systems relative to vehicles in current operation. In addition, subjective response data obtained from vibration simulator studies are presented to illustrate human response characteristics as well as to indicate a laboratory approach for the development of ride-quality criteria.

Stephens, D. G.↗

Window comparator for voltages

Circuit determines whether voltage is within preselected range of voltage levels.Device requires fewer components than previous window comparators and is less susceptible to errors from reference drift. Comparator is useful in process-control circuitry, measuring instruments, and checkout equipment.

Black, J. M.↗