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At least 307 records · Page 17

Hoechst fluorescence intensity can be used to separate viable bromodeoxyuridine-labeled cells from viable non-bromodeoxyuridine-labeled cells

BACKGROUND: 5-Bromo-2'-deoxyuridine (BrdU) is a powerful compound to study the mitotic activity of a cell. Most techniques that identify BrdU-labeled cells require conditions that kill the cells. However, the fluorescence intensity of the membrane-permeable Hoechst dyes is reduced by the incorporation of BrdU into DNA, allowing the separation of viable BrdU positive (BrdU+) cells from viable BrdU negative (BrdU-) cells. METHODS: Cultures of proliferating cells were supplemented with BrdU for 48 h and other cultures of proliferating cells were maintained without BrdU. Mixtures of viable BrdU+ and viable BrdU- cells from the two proliferating cultures were stained with Hoechst 33342. The viable BrdU+ and BrdU- cells were sorted into different fractions from a mixture of BrdU+ and BrdU- cells based on Hoechst fluorescence intensity and the ability to exclude the vital dye, propidium iodide. Subsequently, samples from the original mixture, the sorted BrdU+ cell population, and the sorted BrdU- cell population were immunostained using an anti-BrdU monoclonal antibody and evaluated using flow cytometry. RESULTS: Two mixtures consisting of approximately 55% and 69% BrdU+ cells were sorted into fractions consisting of greater than 93% BrdU+ cells and 92% BrdU- cells. The separated cell populations were maintained in vitro after sorting to demonstrate their viability. CONCLUSIONS: Hoechst fluorescence intensity in combination with cell sorting is an effective tool to separate viable BrdU+ from viable BrdU- cells for further study. The separated cell populations were maintained in vitro after sorting to demonstrate their viability. Copyright 2000 Wiley-Liss, Inc.

Evaluation Studies↗

Development of Testing Station for Prototype Rover Thermal Subsystem

In order to successfully and efficiently explore the moon or other planets, a vehicle must be built to assist astronauts as they travel across the surface. One concept created to meet this need is NASA's Space Exploration Vehicle (SEV). The SEV, a small pressurized cabin integrated onto a 12-wheeled chassis, can support two astronauts up to 14 days. Engineers are currently developing the second generation of the SEV, with the goal of being faster, more robust, and able to carry a heavier payload. In order to function properly, the rover must dissipate heat produced during operation and maintain an appropriate temperature profile inside the rover. If these activities do not occur, components of the rover will start to break down, eventually leading to the failure of the rover. On the rover, these requirements are the responsibility of the thermal subsystem. My project for the summer was to design and build a testing station to facilitate the design and testing of the new thermal subsystem. As the rover develops, initial low fidelity parts can be interchanged for the high fidelity parts used on the rover. Based on a schematic of the proposed thermal system, I sized and selected parts for each of the components in the thermal subsystem. For the components in the system that produced heat but had not yet been finalized or fabricated, I used power resistors to model their load patterns. I also selected all of the fittings to put the system together and a mounting platform to support the testing station. Finally, I implemented sensors at various points in the system to measure the temperature, pressure, and flow rate, and a data acquisition system to collect this information. In the future, the information from these sensors will be used to study the behavior of the subsystem under different conditions and select the best part for the rover.

Burlingame, Kaitlin↗

Lightweight Magnetic Cooler With a Reversible Circulator

A design of a highly efficient and lightweight space magnetic cooler has been developed that can continuously provide remote/distributed cooling at temperatures in the range of 2 K with a heat sink at about 15 K. The innovative design uses a cryogenic circulator that enables the cooler to operate at a high cycle frequency to achieve a large cooling capacity. The ability to provide remote/distributed cooling not only allows flexible integration with a payload and spacecraft, but also reduces the mass of the magnetic shields needed. The active magnetic regenerative refrigerator (AMRR) system is shown in the figure. This design mainly consists of two identical magnetic regenerators surrounded by their superconducting magnets and a reversible circulator. Each regenerator also has a heat exchanger at its warm end to reject the magnetization heat to the heat sink, and the two regenerators share a cold-end heat exchanger to absorb heat from a cooling target. The circulator controls the flow direction, which cycles in concert with the magnetic fields, to facilitate heat transfer. Helium enters the hot end of the demagnetized column, is cooled by the refrigerant, and passes into the cold-end heat exchanger to absorb heat. The helium then enters the cold end of the magnetized column, absorbing heat from the refrigerant, and enters the hot-end heat exchanger to reject the magnetization heat. The efficient heat transfer in the AMRR allows the system to operate at a relatively short cycle period to achieve a large cooling power. The key mechanical components in the magnetic cooler are the reversible circulator and the magnetic regenerators. The circulator uses non-contacting, self-acting gas bearings and clearance seals to achieve long life and vibration- free operation. There are no valves or mechanical wear in this circulator, so the reliability is predicted to be very high. The magnetic regenerator employs a structured bed configuration. The core consists of a stack of thin GGG disks alternating with thin polymer insulating films. The structured bed reduces flow resistance in the regenerator and therefore the pumping work by the cryogenic circulator. This magnetic cooler will enable cryogenic detectors for sensing infrared, x-ray, gamma-ray, and submillimeter radiation in future science satellites, as well as the detector systems in the Constellation-X (Con-X) and the Single Aperture Far-Infrared observatory (SAFIR). Scientific ap p - lica tions for this innovation include cooling for x-ray micro calorimeter spectrometers used for microanalysis, cryogenic particle detectors, and superconducting tunnel junction de tectors for biomolecule mass spectrometry. The cooler can be scaled to provide very large cooling capacities at very low temperatures, ideal for liquid helium and liquid hydrogen productions.

Chen, Weibo↗

Experimental Investigation of Pool Boiling Heat Transfer Enhancement in Microgravity in the Presence of Electric Fields

In boiling high heat fluxes are possible driven by relatively small temperature differences, which make its use increasingly attractive in aerospace applications. The objective of the research is to develop ways to overcome specific problems associated with boiling in the low gravity environment by substituting the buoyancy force with the electric force to enhance bubble removal from the heated surface. Previous studies indicate that in terrestrial applications nucleate boiling heat transfer can be increased by a factor of 50, as compared to values obtained for the same system without electric fields. The goal of our research is to experimentally explore the mechanisms responsible for EHD heat transfer enhancement in boiling in low gravity conditions, by visualizing the temperature distributions in the vicinity of the heated surface and around the bubble during boiling using real-time holographic interferometry (HI) combined with high-speed cinematography. In the first phase of the project the influence of the electric field on a single bubble is investigated. Pool boiling is simulated by injecting a single bubble through a nozzle into the subcooled liquid or into the thermal boundary layer developed along the flat heater surface. Since the exact location of bubble formation is known, the optical equipment can be aligned and focused accurately, which is an essential requirement for precision measurements of bubble shape, size and deformation, as well as the visualization of temperature fields by HI. The size of the bubble and the frequency of bubble departure can be controlled by suitable selection of nozzle diameter and mass flow rate of vapor. In this approach effects due to the presence of the electric field can be separated from effects caused by the temperature gradients in the thermal boundary layer. The influence of the thermal boundary layer can be investigated after activating the heater at a later stage of the research. For the visualization experiments a test cell was developed. All four vertical walls of the test cell are transparent, and they allow transillumination with laser light for visualization experiments by HI. The bottom electrode is a copper cylinder, which is electrically grounded. The copper block is heated with a resistive heater and it is equipped with 6 thermocouples that provide reference temperatures for the measurements with HI. The top electrode is a mesh electrode. Bubbles are injected with a syringe into the test cell through the bottom electrode. The working fluids presently used in the interferometric visualization experiments, water and PF 5052, satisfy requirements regarding thermophysical, optical and electrical properties. A 30kV power supply equipped with a voltmeter allows to apply the electric field to the electrodes during the experiments. The magnitude of the applied voltage can be adjusted either manually or through the LabVIEW data acquisition and control system connected to a PC. Temperatures of the heated block are recorded using type-T thermocouples, whose output is read by a data acquisition system. Images of the bubbles are recorded with 35mm photographic and 16mm high-speed cameras, scanned and analyzed using various software packages. Visualized temperature fields HI allows the visualization of temperature fields in the vicinity of bubbles during boiling in the form of fringes. Typical visualized temperature distributions around the air bubbles injected into the thermal boundary layer in PF5052 are shown. The temperature of the heated surface is 35 C. The temperature difference for a pair of fringes is approximately 0.05 C. The heat flux applied to the bottom surface is moderate, and the fringe patterns are regular. In the image a bubble penetrating the thermal boundary layer is visible. Because of the axial symmetry of the problem, simplified reconstruction techniques can be applied to recover the temperature field. The thermal plume developing above the heated surface for more intensive heating is shown. The temperature distribution in the liquid is clearly 3D, and tomographic techniques have to be applied to recover the temperature distribution in such a physical situation. A sequence of interferometric images showing the temperature distribution around the rising bubble, recorded with a high-speed camera is shown. Again, the temperature distribution is 3D, and a more complex approach to the evaluation, the tomographic reconstruction has to be taken. Measurement of the temperature distribution from the fringe pattern temperature distributions that yield important information regarding heat transfer are determined. Two algorithms that allow the quantitative evaluation of interferometric fringe patterns and the reconstruction of temperature fields during boiling have been developed at the Heat Transfer Laboratory of the Johns Hopkins University. In the first algorithm the bubble is assumed to be axially symmetrical, which significantly reduces the computational effort for quantifying the temperature distribution around the bubble. For this purpose the thermal boundary layer around the bubble is divided into equidistant concentric shells, and the refractive index is assumed to be constant in each of the shells. Since large temperature gradients are expected in the vicinity of the bubble during boiling, the deflection of the light beam cannot be neglected in boiling experiments. Since the exit angle of the light beam is known, this allows to account for the deflections and phase shifts outside the boundary layer (in the bulk fluid and in the windows of the test cell). Three dimensional temperature distributions in the vicinity of the bubble are reconstructed using tomographic techniques. In tomography, the measurement volume is sliced into 2D planes. In the present study these planes are parallel to the heated surface. The objective is to determine the values of the field parameter of interest in form of the field function in these 2D planes. The field parameter is the change of the refractive index of the liquid in the measurement volume caused by temperature changes. By superimposing data for many 2D planes recorded at the same time instant, the 3D temperature distribution in the measurement volume is recovered.

Herman, Cila↗

Control System Development for A Zero Boil-Off Hydrogen Storage Demonstration With Two-Stage Active Cooling

A NASA team is designing and building a test article to demonstrate the long duration storage of liquid hydrogen via active cooling (cryocoolers) while implementing a two-stage cooling approach. This activity is one of a large portfolio of NASA’s Space Technology Mission Directorate funded activities which focuses on the technology maturation needed for long duration storage of cryogenic liquid in-space. The current state-of-the-art for liquid hydrogen storage on-orbit is on the order of hours while NASA’s planned missions require storage for months, or even years. To enable such a long duration, Zero Boil-Off conditions must be achieved which requires passive technologies to minimize environmental heat loads, but also active cooling to intercept and reject the remaining heat to deep space. The implementation of active cooling results in a significant amount of dry mass added to the vehicle. Utilizing a Two-Stage Cooling approach, analysis and testing to date indicates the Zero Boil-Off of liquid hydrogen can be achieved with less mass and electrical power relative to a single-stage cooling approach where only one cryocooler is used. This activity will demonstrate the fully integrated suite of technologies needed to enable the Two-Stage Cooling approach which includes Multi-Layer Insulation blankets, Low Conductivity Structures, a Tube-On-Tank Heat Exchanger, Tube-On-Shield Heat Exchanger and a Two-Stage Cryogenerator (both 90 Kelvin and 20 Kelvin) with each stage having an independently controlled circulation loop. The bulk of the heat load is intercepted by the 90 Kelvin loop via a thin foil heat exchanger internal to the insulation blankets (also known as a Broad Area Cooling Shield), with the remainder removed at cryofluid temperature (20 Kelvin) by tubes directly welded to the outer tank surface. In this paper, we examine the challenges of controlling the system, and describe the development of control algorithms and software for future testing with liquid hydrogen. The interactions between the cryofans which control circulation loop mass flow rates, electrical heaters which vary the cryocooler lift to simulate the operation of variable-lift flight units, tank heaters which can vary the overall heat load, and the effects of changes in circulation loop pressure as temperatures change, can be complex and must be well understood to maintain steady-state propellant conditions and achieve Zero Boil-Off. Parallel PID loops and watchdog programs implemented on the user interface system will help bring the systems to steady state operation and keep them at selected operating points within acceptable error, while avoiding runaway feedback loops.

cryogenics↗

Occurrence of Large Geomagnetically Induced Currents Within the EPRI SUNBURST Monitoring Network

Space weather, a natural hazard, can adversely impact human technological assets. High-voltage electric power transmission grids constitute one of the most critical technological systems vulnerable to space weather driven geomagnetically induced currents (GICs). One of the major challenges pertaining to the study of GICs over the continental United States has been the availability of GIC measurements, which are critical for validation of geoelectric field and power flow models, for example. In this study, we analyze GIC measurements collected at 17 Electrical Power Research Institute (EPRI) SUNBURST transformer locations across the United States for which a GIC value of 10 A or greater was recorded. This data set includes 52 individual geomagnetic storms with Kp index 6 and above during the period from 2010 to 2021. The analysis confirms that there is a good correlation between the number of geomagnetic storms per year and the number of recorded GIC events. Our results also show that about 76% of the top 17 GIC events are associated with the storm main phase, while only 24% are attributed to storm sudden commencements. In addition, it is shown, for the first time, that mid-latitude positive bays can cause large GICs over the continental United States. Finally, this study shows that the largest measured GIC event in the data set was associated with a localized intense dB/dt structure, which could be attributed to substorm activity.

Chigomezyo M. Ngwira↗

Acoustic Performance of an Advanced Model Turbofan in Three Aeroacoustic Test Facilities

A model advanced turbofan was acoustically tested in the NASA Glenn 9- by 15-Foot-Low-Speed Wind Tunnel (LSWT), and in two other aeroacoustic facilities. The Universal Propulsion Simulator (UPS) fan was designed and manufactured by the General Electric Aircraft Engines (GEAE) Company, and featured active core, as well as bypass, flow paths. The reference test configurations were with the metal, M4, rotor with hardwall and treated bypass flow ducts. The UPS fan was tested within an airflow at a Mach number of 0.20 (limited flow data were also acquired at a Mach number of 0.25) which is representative of aircraft takeoff and approach conditions. Comparisons were made between data acquired within the airflow (9x15 LSWT and German-Dutch Wind Tunnel (DNW)) and outside of a free jet (Boeing Low Speed Aero acoustic Facility (LSAF) and DNW). Sideline data were acquired on an 89-in. (nominal 4 fan diameters) sideline using the same microphone assembly and holder in the 9x15 LSWT and DNW facilities. These data showed good agreement for similar UPS operating conditions and configurations. Distortion of fan spectra tonal content through a free jet shear layer was documented, suggesting that in-flow acoustic measurements are required for comprehensive fan noise diagnostics. However, there was good agreement for overall sound power level (PWL) fan noise measurements made both within and outside of the test facility airflow.

Woodward, Richard P.↗

X-ray emission associated with radio galaxies in the Perseus cluster

In this paper, we report on new x-ray observations of the Perseus cluster made using four separate pointings of the Roentgen Satellite (ROSAT) Positron Sensitive Proportional Counter (PSPC). We searched for x-ray emission associated with 16 radio galaxies and detected six above 3 sigma. We made use of the PSPC spectra to determine if the x-ray emission associated with radio galaxies in Perseus is thermal or nonthermal in origin (i.e., hot gas or an active galactic nuclei (AGN)). For the head-tail radio galaxy IC 310, we find that the data are best fit by a power law model with an unusually large spectral index alpha = 2.7. This is consistent with its unresolved spatial structure. On the other hand, a second resolved x-ray source associated with another radio galaxy 2.3 Mpc from the Perseus center (V Zw 331) is best fit by a thermal model. For three sources with insufficient flux for a full spectral analysis, we calculated hardness ratios. On this basis, the x-ray emission associated with the well known head-tail source NGC 1265 is consistent with thermal radiation. The x-ray spectra of UGC 2608 and UGC 2654 probably arise from hot gas, although very steep power-law spectra (alpha greater than 3.2) are also possible. The spectrum of NGC 1275 is quite complex due to the presence of an AGN and the galaxy's location at the center of a cluster cooling flow.

Rhee, George↗

Potential vorticity index

Using standard data analysis techniques, researchers explore the links between disturbance growth and quasi-geostrophic potential vorticity (PV) gradients; appearance and disappearance of cutoff lows and blocking highs and their relation to a zonal index (properly defined in terms of PV); and teleconnections between different flow patterns and their relation to the zonal index. It was found that the PV index and the eddy index correlate better than a zonal index (defined by zonal wind) and the eddy index. In the frequency domain there are three frequencies (.03, .07 and .17 cpd (cycle per day) corresponding to periods of 33, 14 and 6 days) at which PV index and the eddy index exhibit local maxima. The high correlation found at periods of 33 days is mainly due to eddy activity at high latitudes while the local correlation maxima found at the shorter periods are mainly due mid-latitude eddy activity. The correlation between the PV index and the geopotential height anomaly at 500 mb, at each grid point in the Northern Hemisphere, shows the existence of most of the teleconnection patterns summarized by Wallace and Gutzler (1981): the North Atlantic Oscillation, the North Pacific Oscillation, and the Pacific/North American patterns. Results show that the Isentropic Potential Vorticity (IPV) analysis can be a very useful and powerful tool when used to understand the dynamics of several large scale atmospheric systems. Although the data are limited to only one winter, and it is difficult to assess the statistical significance of the correlation coefficients presented here, the results are encouraging from physical viewpoint.

Barcilon, Albert↗

Vortex motion phase separator for zero gravity liquid transfer

A vortex motion phase separator is disclosed for transferring a liquid in a zero gravity environment while at the same time separating the liquid from vapors found within either the sender or the receiving tanks. The separator comprises a rigid sender tank having a circular cross-section and rigid receiver tank having a circular cross-section. A plurality of ducts connects the sender tank and the receiver tank. Disposed within the ducts connecting the receiver tank and the sender tank is a pump and a plurality of valves. The pump is powered by an electric motor and is adapted to draw either the liquid or a mixture of the liquid and the vapor from the sender tank. Initially, the mixture drawn from the sender tank is directed through a portion of the ductwork and back into the sender tank at a tangent to the inside surface of the sender tank, thereby creating a swirling vortex of the mixture within the sender tank. As the pumping action increases, the speed of the swirling action within the sender tank increases creating an increase in the centrifugal force operating on the mixture. The effect of the centrifugal force is to cause the heavier liquid to migrate to the inside surface of the sender tank and to separate from the vapor. When this separation reaches a predetermined degree, control means is activated to direct the liquid conveyed by the pump directly into the receiver tank. At the same time, the vapor within the receiver tank is directed from the receiver tank back into the sender tank. This flow continues until substantially all of the liquid is transferred from the sender tank to the receiver tank.

Howard, Frank S.↗

Catalyst for Carbon Monoxide Oxidation

In many applications, it is highly desirable to operate a CO2 laser in a sealed condition, for in an open system the laser requires a continuous flow of laser gas to remove the dissociation products that occur in the discharge zone of the laser, in order to maintain a stable power output. This adds to the operating cost of the laser, and in airborne or space applications, it also adds to the weight penalty of the laser. In a sealed CO2 laser, a small amount of CO2 gas is decomposed in the electrical discharge zone into corresponding quantities of CO and O2. As the laser continues to operate, the concentration of CO2 decreases, while the concentrations of CO and O2 correspondingly increase. The increasing concentration of O2 reduces laser power, because O2 scavenges electrons in the electrical discharge, thereby causing arcing in the electric discharge and a loss of the energetic electrons required to boost CO2 molecules to lasing energy levels. As a result, laser power decreases rapidly. The primary object of this invention is to provide a catalyst that, by composition of matter alone, contains chemisorbed water within and upon its structure. Such bound moisture renders the catalyst highly active and very long-lived, such that only a small quantity of it needs to be used with a CO2 laser under ambient operating conditions. This object is achieved by a catalyst that consists essentially of about 1 to 40 percent by weight of one or more platinum group metals (Pt, Pd, Rh, Ir, Ru, Os, Pt being preferred); about 1 to 90 percent by weight of one or more oxides of reducible metals having multiple valence states (such as Sn, Ti, Mn, Cu, and Ce, with SnO2 being preferred); and about 1 to 90 percent by weight of a compound that can bind water to its structure (such as silica gel, calcium chloride, magnesium sulfate, hydrated alumina, and magnesium perchlorate, with silica gel being preferred). Especially beneficial results are obtained when platinum is present in the catalyst composition in an amount of about 5 to 25 (especially 7) percent by weight, SnO2 is present in an amount of about 30 to 40 (especially 40) percent by weight, and silica gel is present in an amount of 45 to 55 (especially 50) percent by weight. The composition of this catalyst was suggested by preliminary experiments in which a Pt/SnO2 catalyst was needed for bound water to enhance its activity. These experimental results suggested that if the water were bound to the surface, this water would enhance and prolong catalyst activity for long time periods. Because the catalyst is to be exposed to a laser gas mixture, and because a CO2 laser can tolerate only a very small amount of moisture, a hygroscopic support for the catalyst would provide the needed H2O into the gas. Silica gel is considered to be superior because of its property to chemisorb water on its surface over a wide range of moisture content.

Davis, Patricia↗

Risk-Based Fire Safety Experiment Definition for Manned Spacecraft

Risk methodology is used to define experiments to be conducted in space which will help to construct and test the models required for accident sequence identification. The development of accident scenarios is based on the realization that whether damage occurs depends on the time competition of two processes: the ignition and creation of an adverse environment, and the detection and suppression activities. If the fire grows and causes damage faster than it is detected and suppressed, then an accident occurred. The proposed integrated experiments will provide information on individual models that apply to each of the above processes, as well as previously unidentified interactions and processes, if any. Initially, models that are used in terrestrial fire risk assessments are considered. These include heat and smoke release models, detection and suppression models, as well as damage models. In cases where the absence of gravity substantially invalidates a model, alternate models will be developed. Models that depend on buoyancy effects, such as the multizone compartment fire models, are included in these cases. The experiments will be performed in a variety of geometries simulating habitable areas, racks, and other spaces. These simulations will necessitate theoretical studies of scaling effects. Sensitivity studies will also be carried out including the effects of varying oxygen concentrations, pressures, fuel orientation and geometry, and air flow rates. The experimental apparatus described herein includes three major modules: the combustion, the fluids, and the command and power modules.

Apostolakis, G. E.↗

Development of a Deep-Penetrating, Compact Geothermal Heat Flow System for Robotic Lunar Geophysical Missions

Geothermal heat flow measurements are a high priority for the future lunar geophysical network missions recommended by the latest Decadal Survey of the National Academy. Geothermal heat flow is obtained as a product of two separate measurements of geothermal gradient and thermal conductivity of the regolith/soil interval penetrated by the instrument. The Apollo 15 and 17 astronauts deployed their heat flow probes down to 1.4-m and 2.3-m depths, respectively, using a rotary-percussive drill. However, recent studies show that the heat flow instrument for a lunar mission should be capable of excavating a ~3-m deep hole to avoid the effect of potential long-term changes of the surface thermal environment. For a future robotic geophysical mission, a system that utilizes a rotary/percussive drill would far exceed the limited payload and power capacities of the lander/rover. Therefore, we are currently developing a more compact heat flow system that is capable of 3-m penetration. Because the grains of lunar regolith are cohesive and densely packed, the previously proposed lightweight, internal hammering systems (the so-called moles ) are not likely to achieve the desired deep penetration. The excavation system for our new heat flow instrumentation utilizes a stem which winds out of a pneumatically driven reel and pushes its conical tip into the regolith. Simultaneously, gas jets, emitted from the cone tip, loosen and blow away the soil. Lab tests have demonstrated that this proboscis system has much greater excavation capability than a mole-based heat flow system, while it weighs about the same. Thermal sensors are attached along the stem and at the tip of the penetrating cone. Thermal conductivity is measured at the cone tip with a short (1- to 1.5-cm long) needle sensor containing a resistance temperature detector (RTD) and a heater wire. When it is inserted into the soil, the heater is activated. Thermal conductivity of the soil is obtained from the rate of temperature increase during the heating. By stopping during the excavation, it is possible to measure thermal conductivities at different depths. The gas jets are turned off when the penetrating cone reaches the target depth. Then, the stem pushes the needle sensor into the undisturbed soil at the bottom of the hole and carries out a thermal conductivity measurement. When the measurement is complete, the system resumes excavation. RTDs, placed along the stem at short (approx 30 cm) intervals, will monitor long-term temperature stability of the subsurface. Temperature in the shallow subsurface would fluctuate with the diurnal, annual, and precession cycles of the Moon. These thermal waves penetrate to different depths into the regolith. Longterm monitoring of the subsurface temperature would allow us to accurately delineate these cyclic signals and separate them from the signal associated with the outward flow of the Moon s endogenic heat. Further, temperature toward bottom of the 3-m hole should be fairly stable after the heat generated during the excavation dissipates into the surrounding soil. The geothermal gradient may be determined reliably from temperature measurements at the RTDs near the bottom. In order to minimize the heat conduction along the stem from affecting the geothermal gradient measurements, we plan to use low-conductive materials for the stem and develop a mechanism to achieve close coupling between the RTDs and the wall of the excavated hole.

Nagihara, Seiichi↗

Rapid Cycle Amine 4.0 System Development

Carbon dioxide (CO2) and humidity control is a key function of the spacesuit Portable Life Support Subsystem (PLSS). The latest spacesuit design utilizes a pressure swing bed containing a solid amine sorbent to scrub CO2 and humidity produced by the crewmember from the ventilation loop. The solid amine sorbent removes CO2 and humidity from the ventilation loop through a chemisorption mechanism. Simultaneously, CO2 and humidity are desorbed to vacuum via the two-bed pressure swing system during operation. This continuous regeneration allows for longer Extravehicular Activity (EVA) times while minimizing maintenance, consumables, and power usage. This concept has been in development for over 20 years, during which many prototypes and design iterations have been fabricated and evaluated. This paper discusses the recent Rapid Cycle Amine (RCA) design, development, and testing that has been done to advance the technology. The most recent fabrication is the RCA 3.0. It was delivered to NASA Johnson Space Center in 2015 and has since been tested at the component level and at the integrated PLSS level. The purpose of the component level testing was to understand CO2 removal capacity, end-of-life performance, and establish a baseline performance prior to integrated testing. The RCA 3.0 was integrated into the Exploration Extravehicular Mobility Unit (xEMU) PLSS for a comprehensive Design Verification Test (DVT) series. The integrated testing provided the benefit of characterizing the impact performance degradation has on the system. Many of the RCA requirements were developed through analysis, based on the limitations of the PLSS and, in some cases, assumptions about the system and flow behavior. Integrated testing was an opportunity to validate those requirements and assumptions. While the RCA 3.0 was being tested, the next design iteration, RCA 4.0, was under development. The RCA 4.0 preliminary design phase began in late-2019 and shortly after the Preliminary Design Review, the critical design phase began in 2021. The design work has concluded with the completion of a Pre-Manufacturing Review in 2022. This paper provides an overview of the RCA 4.0 system design, summarizes the results of integrated RCA 3.0 testing, and discusses the pertinence of driving requirements such as mass, volume, and power consumption.

Kelsey Bloom↗

Space Shuttle Main Engine plume spectral monitoring preliminary results

In order to provide the information necessary to develop a rocket engine ground test failure detection monitor which keys on anomalous activity in the plume, a series of experimental observations were made during single engine Space Shuttle Main Engine (SSME) tests. The observations were conducted from the 220 nm wavelength in the ultraviolet out to the 15 micron wavelength in the infrared. Four types of instruments were employed including analog autoranging narrow band radiometers, optical multichannel analyzers, mechanical scanning and film plate spectrometers, and imaging cameras. Some results included: strong OH radiation in the UV up to vibrational transition state four; Na and K radiation lines in all phases of engine operation; reaction of some of the instruments to minor engine anomalies; good shock structure imaging; striations in the flow in the 310 nm OH band; and detection of gross changes in engine operating state such as start or shutdown.

Cikanek, H. A., III↗

Identification of low-frequency fluctuations in the terrestrial magnetosheath

On the basis of magnetohydrodynamic (MHD) theory we develop a scheme for distinguishing among the four low-frequency modes which may propagate in a high-beta anisotropic plasma such as the magnetosheath: the fast and slow magnetosonic, the Alfven, and mirror modes. We use four parameters: the ratio of transverse to compressional powers in the magnetic field, the ratio of the wave powers in the thermal pressure and in the magnetic field, the ratio of the perturbations in the thermal and magnetic pressures, and the ratio of the wave powers in the velocity and in the magnetic field. In the test case of an Active Magnetospheric Particle Tracer Explorers/Ion Release Module (AMPTE/IRM) magnetosheath pass near the Sun-Earth line downstream of a quasi-perpendicular shock, the four modes can be clearly distinguished both spatially and spectrally. Near the bow shock, the waves are Alfvenic in a large frequency range, 1 to 100 mHz. In the middle and inner magnetosheath, the waves below 10 mHz are Alfvenic. The fast mode waves occur in the higher-frequency end of the enhanced spectrum, 80 mHz for the middle magnetosheath and 55 mHz for the inner sheath. The wave enhancement in the intermediate frequencies is slow modes in the inner sheath and mirror modes in the middle sheath. This confirms the earlier report of the existence of the slow mode waves near the magnetopause. These slow waves provide evidence that the magnetopause is an active source of the waves in the sheath. We also show that the measured frequency of a wave is close to an invariant if the magnetosheath flow is in a steady state. Therefore changes in the frequencies of enhanced waves indicate emergence, or damping, or mode conversion of the waves.

Song, P.↗

An Active Smart Material Control System for F/A-18 Buffet Alleviation

The vertical tail buffet problem of fighter aircraft occurs at high angles of attack when the vortical flow breaks down ahead of the vertical tails resulting in unsteady and unbalanced pressure loads on the vertical tails. The buffet loads imposed upon the vertical tails resulted in a premature fatigue failure of the tails, and consequently limits the performance and super maneuverability of twin-tail fighter aircraft. An active smart material control system using distributed piezoelectric actuators has been developed for buffet alleviation and is presented. The inboard and outboard surfaces of the vertical tail are equipped with piezoelectric actuators to control the buffet responses in the first bending and torsion modes. The electrodynamics of the piezoelectric actuators are expressed with a three-dimensional finite-element model. A single-input-single-output controller is designed to drive the active piezoelectric actuators. High-fidelity multidisciplinary analysis modules for the fluid dynamics, structure dynamics, electrodynamics of the piezoelectric actuators, control law, fluid structure interfacing, and grid motion are integrated into a multidisciplinary computing environment that controls the temporal synchronization of the analysis modules. At 30 degree angle of attack, RMS values of tip acceleration are reduced by as much as 12%. The peak values of the power spectral density of tail-tip acceleration are reduced by as much as 22% in the first bending mode and by as much as 82% in the first torsion mode. The actively controlled piezoelectric actuators were also effective in adding damping at wide range of angles of attack.

Sheta, Essam F.↗

Low-Loss, High-Isolation Microwave Microelectromechanical Systems (MEMS) Switches Being Developed

Switches, electrical components that either permit or prevent the flow of electricity, are the most important and widely used electrical devices in integrated circuits. In microwave systems, switches are required for switching between the transmitter and receiver; in communication systems, they are needed for phase shifters in phased-array antennas, for radar and communication systems, and for the new class of digital or software definable radios. Ideally, switches would be lossless devices that did not depend on the electrical signal's frequency or power, and they would not consume electrical power to change from OFF to ON or to maintain one of these two states. Reality is quite different, especially at microwave frequencies. Typical switches in microwave integrated circuits are pin diodes or gallium arsenide (GaAs) field-effect transistors that are nonlinear, with characteristics that depend on the power of the signal. In addition, they are frequency-dependent, lossy, and require electrical power to maintain a certain state. A new type of component has been developed that overcomes most of these technical difficulties. Microelectromechanical (MEMS) switches rely on mechanical movement as a response to an applied electrical force to either transmit or reflect electrical signal power. The NASA Glenn Research Center has been actively developing MEMS for microwave applications for over the last 5 years. Complete fabrication procedures have been developed so that the moving parts of the switch can be released with near 100-percent yield. Moreover, the switches fabricated at Glenn have demonstrated state-of-the-art performance. A typical MEMS switch is shown. The switch extends over the signal and ground lines of a finite ground coplanar waveguide, a commonly used microwave transmission line. In the state shown, the switch is in the UP state and all the microwave power traveling along the transmission line proceeds unimpeded. When a potential difference is applied between the cantilever and the transmission line, the cantilever is pulled downward until it connects the signal line to the ground planes, creating a short circuit. In this state, all the microwave power is reflected. The graph shows the measured performance of the switch, which has less than 0.1 dB of insertion loss and greater than 30dB of isolation. These switches consume negligible electrical power and are extremely linear. Additional research is required to address reliability and to increase the switching speed.

Ponchak, George E.↗