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At least 289 records · Page 16

Climatic clustering and longitudinal analysis with impacts on food, bioenergy, and pandemics

Predicted growth in world population will put unparalleled stress on the need for sustainable energy and global food production, as well as increase the likelihood of future pandemics. In this work, we identify high-resolution environmental zones in the context of a changing climate and predict longitudinal processes relevant to these challenges. We do this using exhaustive vector comparison methods that measure the climatic similarity between all locations on earth at high geospatial resolution relative to global-scale analyses. The results are captured as networks, in which edges between geolocations are defined if their historical climate similarities exceed a threshold. We apply Markov clustering and our novel Correlation of Correlations method to the resulting climatic networks, which provides unprecedented agglomerative and longitudinal views of climatic relationships across the globe. The methods performed here resulted in the fastest (9.37x10 18 operations/sec) and one of the largest (168.7x10 21 operations) scientific computations ever performed, with more than 100 quadrillion edges considered for a single climatic network. Our climatic analysis reveals areas of the world experiencing rapid environmental changes, which can have important implications for global carbon fluxes and zoonotic spillover events. Correlation and network analyses of this kind are widely applicable across computational and predictive biology domains, including systems biology, ecology, carbon cycles, biogeochemistry, and zoonosis research.

59 BASIC BIOLOGICAL SCIENCES↗

Exploring the impact of high-precision top-quark pair production data on the structure of the proton at the LHC

The impact of recent LHC top-quark pair production single differential cross section measurements at 13 TeV collision energy on the structure of the proton is explored. In particular, the impact of these high-precision data on the gluon and other parton distribution functions (PDFs) of the proton at intermediate and large partonic momentum fraction x is analyzed. This study extends the CT18 global analysis framework to include these new data. The interplay between top-quark pair and inclusive jet production as well as other processes at the LHC, is studied. In addition, a study of the impact of scale choice on the theory description of the new 13 TeV t t ¯ measurements is performed. Published by the American Physical Society 2024

Astronomy & Astrophysics↗

Adaptive Time-Division Multiplexing Driving System for Solid State Lighting with Multiple Tunable Channels

Next generation solid state lighting enables unlimited control of light and serve much broader functions beyond the basic lighting for illuminance. The emerging lighting for productivity, well-being, healthcare, and growth have revolutionized the scope of lighting. To empower this unlimited controllability, a multi-channel tunable power-electronics driver is essential but has not been satisfactorily addressed so far. Existing solutions are lossy, large, and expensive, due to introduction of additional buck power conversion stages. This paper proposes a time-division multiplexing (TDM) LED driving system which eliminates those buck stages. The proposed design can directly pair with off-the-shelf pulse width modulated (PWM) controllers and since it inherits entire feature sets, it can achieve low loss at standby and at dimmed condition. The simple analog implementation avoids usage of expensive DSPs/MCU. GaN’s high switching frequency capability can push up the multiplexing frequency so a GaN-based flyback was built and proved the concept.

Xue, Lincoln↗

Medium-Voltage Isolated Auxiliary Power Supply Design for High Insulation Capability, Ultra-Low Coupling Capacitance, and Small Size

To fully utilize the benefits of medium-voltage (MV) silicon carbide (SiC)-based converter and achieve high power density, the isolated auxiliary power supply (IAPS) needs to be as small as possible. However, the high insulation and low coupling capacitance requirements, considering the high voltage and high dv / dt , are constraints of size reduction. This article proposes an MV IAPS design for high insulation capability, ultra-low coupling capacitance, and small size. The topology selection, efficiency, load regulation, coupling capacitance, and size reduction considerations are discussed. A 2.5 W MV IAPS is designed and built for a 10 kV SiC mosfet -based 13.8 kV/100 kW three-phase converter, achieving a partial discharge (PD) inception voltage of higher than 15 kV rms, ultra-low coupling capacitance of 1.03 pF, and smaller size than that of the commercial products as well as the state-of-art research prototypes. Experimental results of PD test, 10 kV SiC mosfet -based half-bridge test, and 13.8 kV/100 kVA three-phase converter full rating test are conducted for design validation.

42 ENGINEERING↗

Silicate minerals as a direct source of limiting nutrients: Siderophore synthesis and uptake promote ferric iron bioavailability from olivine and microbial growth

Iron is a micronutrient critical to fundamental biological processes including respiration and photosynthesis, and it can therefore impact primary and heterotrophic productivity. Yet in oxic environments, iron is highly insoluble, rendering it, in principle, unavailable as a nutrient for biological growth. Life has “solved” this problem via the invention of iron chelates, known as siderophores, that keep iron available for microbial productivity. In this work, we examined the impact of siderophore synthesis on the speciation, mobility, and bioavailability of iron from rock-forming silicate minerals—shedding new light on the mechanisms by which microbes use mineral substrates to support primary productivity, as well as the consequent effects on silicate dissolution. Growth experiments were performed with Shewanella oneidensis MR-1 in an oxic, iron-depleted minimal medium, amended with olivine minerals as the sole source of iron. Experiments included the wild-type strain MR-1, and a siderophore synthesis gene deletion mutant strain (ΔMR-1). Relative to MR-1, ΔMR-1 exhibited a very pronounced growth penalty and an extended lag phase. However, substantial growth of ΔMR-1, comparable to MR-1 growth, was observed when the mutant strain was provided with siderophores in the form of either filtrate from a well-grown MR-1 culture, or commercially available deferoxamine. These observations suggest that siderophores are critical for S. oneidensis to acquire iron from olivine. Growth-limiting concentrations of deferoxamine amendments were observed to be ≤5–10 µM, concentrations significantly lower than previously recorded as necessary to impact mineral dissolution rates. X-ray photoelectric spectroscopy analyses of the incubated olivine surfaces suggest that siderophores deplete mineral surface layers of ferric iron. Combined, these results demonstrate that low micromolar concentrations of siderophores can effectively mobilize iron bound within silicate minerals, supporting very significant biological growth in limiting environments. The specific mechanism would involve siderophores removing a protective layer of nanometer-thick iron oxides, enhancing silicate dissolution and nutrient bioavailability.

59 BASIC BIOLOGICAL SCIENCES↗

Microfluidic droplets with amended culture media cultivate a greater diversity of soil microorganisms

ABSTRACT Uncultivated but abundant soil microorganisms have untapped potential for producing broad ranges of natural products, as well as for bioremediation. However, cultivating soil microorganisms while maintaining a broad microorganism diversity to enable phenotyping and functional analysis of as diverse individual isolates as possible remains challenging. In this study, we developed and tested the ability of several culture media formulations that contain defined soil metabolites or soil extracts to maintain microorganism diversity during culture. We also assessed their performance in microfluidic droplet cultivation where single-soil microorganism isolates were encapsulated and cultivated in picoliter-volume water-in-oil emulsion droplets to enable clonal growth needed for downstream functional analyses. Our results show that droplet cultivation with media supplemented by soil extract or soil metabolites enables the recovery of soil microorganisms with higher diversity (up to 1.5-fold higher richness) compared to bulk cultivation methods. Importantly, 1.7-fold more of less abundant (<1%) phyla and 11-fold more of unique genera were recovered, demonstrating the utility of this method for interrogating highly diverse soil microorganisms for broad ranges of applications. IMPORTANCE Although soil microorganisms hold a significant value in bioproduction and bioremediation, only a small fraction—less than 1%—can be cultured under specific media and cultivation conditions. This indicates that there are ample opportunities in harvesting the diverse environmental microorganisms if isolating and recovering these uncultured microorganisms are possible. This paper presents a new cultivation technique composed of isolating single-soil microorganism cell from anin situsoil microorganism community in microfluidic droplets and conducting in-droplet cultivation in media supplemented by soil extract or soil metabolites. This method enables the recovery of a broader diversity of the original microorganism community, laying the groundwork for a high-throughput phenotyping of these diverse microorganisms from their natural habitats.

Biotechnology & Applied Microbiology↗

Genomic analysis and identification of a novel superantigen, SargEY, in Staphylococcus argenteus isolated from atopic dermatitis lesions

During surveillance of Staphylococcus aureus in lesions from patients with atopic dermatitis (AD), we isolated Staphylococcus argenteus, a species registered in 2011 as a new member of the genus Staphylococcus and previously considered a lineage of S. aureus. Genome sequence comparisons between S. argenteus isolates and representative S. aureus clinical isolates from various origins revealed that the S. argenteus genome from AD patients closely resembles that of S. aureus causing skin infections. We previously reported that 17%–22% of S. aureus isolated from skin infections produce staphylococcal enterotoxin Y (SEY), which predominantly induces T-cell proliferation via the T-cell receptor (TCR) Vα pathway. Complete genome sequencing of S. argenteus isolates revealed a gene encoding a protein similar to superantigen SEY, designated as SargEY, on its chromosome. Population structure analysis of S. argenteus revealed that these isolates are ST2250 lineage, which was the only lineage positive for the SEY-like gene among S. argenteus. Recombinant SargEY demonstrated immunological cross-reactivity with anti-SEY serum. SargEY could induce proliferation of human CD4 + and CD8 + T cells, as well as production of TNF-α and IFN-γ. SargEY showed emetic activity in a marmoset monkey model. S arg EY and SET (a phylogenetically close but uncharacterized SE) revealed their dependency on TCR Vα in inducing human T-cell proliferation. Additionally, TCR sequencing revealed other previously undescribed Vα repertoires induced by SEH. S arg EY and SEY may play roles in exacerbating the respective toxin-producing strains in AD.

59 BASIC BIOLOGICAL SCIENCES↗

WISDEM® v3.4.1(Wind Plant Integrated System Design and Engineering Model) [SWR-14-05]

The Wind Plant Integrated System Design and Engineering Model (WISDEM®) is a set of models for assessing overall wind plant cost of energy (COE). The models use wind turbine and plant cost and energy production as well as financial models to estimate COE and other wind plant system attributes. WISDEM® is accessed through Python, is built using OpenMDAO, and uses several sub-models that are also implemented within OpenMDAO. These sub-models can be used independently but they are required to use the overall WISDEM® turbine design capability. Please install all of the pre-requisites prior to installing WISDEM® by following the directions below. For additional information about the NWTC effort in systems engineering that supports WISDEM® development, please visit the official NREL systems engineering for wind energy website. https://www.nlr.gov/wind/systems-engineering

Dykes, Katherine↗

WISDEM® v3.15.2 2024 (Wind-Plant Integrated System Design and Engineering Model) [SWR-14-05]

The Wind-Plant Integrated System Design and Engineering Model (WISDEM®) is a set of models for assessing overall wind plant cost of energy (COE). The models use wind turbine and plant cost and energy production as well as financial models to estimate COE and other wind plant system attributes. WISDEM® is accessed through Python, is built using OpenMDAO, and uses several sub-models that are also implemented within OpenMDAO. These sub-models can be used independently but they are required to use the overall WISDEM® turbine design capability. Please install all of the pre-requisites prior to installing WISDEM® by following the directions below. For additional information about the NWTC effort in systems engineering that supports WISDEM® development, please visit the official NREL systems engineering for wind energy website. https://www.nrel.gov/wind/systems-engineering.html

Dykes, Katherine↗

Plasma Assisted Combustion and Chemical Processing: Chapter 9 - Plasma Diagnostics

Plasma dynamics and chemistry have a broad range of timescales from picoseconds to milliseconds. In addition, it involves nonequilibrium energy transfer between electrons, ions, electronically and vibrationally excited states, radicals, intermediate species, and reactants and products as well as surface charges and chemistry. To understand plasma physics and chemistry, it is essential to conduct time and space resolved, quantitative detection of nonequilibrium temperature distributions, electron energy and number density, electric field, and species concentrations. There are enormous publications and review articles on this subject. The focus of this chapter is to be placed on the most recent progress in gas phase plasma properties and chemistry, especially on optical emission spectroscopy, laser absorption spectroscopy, Faraday rotational spectroscopy, Raman and Thompson scattering, femtosecond and picosecond (fs/ps) coherent anti-Stokes Raman scattering (CARS) spectroscopy, and electric field-induced second harmonic generation methods.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗

Quantification of Ebola virus replication kinetics in vitro

Mathematical modelling has successfully been used to provide quantitative descriptions of many viral infections, but for the Ebola virus, which requires biosafety level 4 facilities for experimentation, modelling can play a crucial role. Ebola virus modelling efforts have primarily focused on in vivo virus kinetics, e.g., in animal models, to aid the development of antivirals and vaccines. But, thus far, these studies have not yielded a detailed specification of the infection cycle, which could provide a foundational description of the virus kinetics and thus a deeper understanding of their clinical manifestation. Here, we obtain a diverse experimental data set of the Ebola virus infection in vitro, and then make use of Bayesian inference methods to fully identify parameters in a mathematical model of the infection. Our results provide insights into the distribution of time an infected cell spends in the eclipse phase (the period between infection and the start of virus production), as well as the rate at which infectious virions lose infectivity. We suggest how these results can be used in future models to describe co-infection with defective interfering particles, which are an emerging alternative therapeutic.

59 BASIC BIOLOGICAL SCIENCES↗

The genome-scale metabolic model for the purple non-sulfur bacterium Rhodopseudomonas palustris Bis A53 accurately predicts phenotypes under chemoheterotrophic, chemoautotrophic, photoheterotrophic, and photoautotrophic growth conditions

The purple non-sulfur bacterium Rhodopseudomonas palustris is recognized as a critical microorganism in the nitrogen and carbon cycle and one of the most common members in wastewater treatment communities. This bacterium is metabolically extremely versatile. It is capable of heterotrophic growth under aerobic and anaerobic conditions, but also able to grow photoautotrophically as well as mixotrophically. Therefore R . palustris can adapt to multiple environments and establish commensal relationships with other organisms, expressing various enzymes supporting degradation of amino acids, carbohydrates, nucleotides, and complex polymers. Moreover, R . palustris can degrade a wide range of pollutants under anaerobic conditions, e.g., aromatic compounds such as benzoate and caffeate, enabling it to thrive in chemically contaminated environments. However, many metabolic mechanisms employed by R . palustris to breakdown and assimilate different carbon and nitrogen sources under chemoheterotrophic or photoheterotrophic conditions remain unknown. Systems biology approaches, such as metabolic modeling, have been employed extensively to unravel complex mechanisms of metabolism. Previously, metabolic models have been reconstructed to study selected capabilities of R . palustris under limited experimental conditions. Here, we developed a comprehensive metabolic model (M-model) for R . palustris Bis A53 ( i DT1294) consisting of 2,721 reactions, 2,123 metabolites, and comprising 1,294 genes. We validated the model using high-throughput phenotypic, physiological, and kinetic data, testing over 350 growth conditions. i DT1294 achieved a prediction accuracy of 90% for growth with various carbon and nitrogen sources and close to 80% for assimilation of aromatic compounds. Moreover, the M-model accurately predicts dynamic changes of growth and substrate consumption rates over time under nine chemoheterotrophic conditions and demonstrated high precision in predicting metabolic changes between photoheterotrophic and photoautotrophic conditions. This comprehensive M-model will help to elucidate metabolic processes associated with the assimilation of multiple carbon and nitrogen sources, anoxygenic photosynthesis, aromatic compound degradation, as well as production of molecular hydrogen and polyhydroxybutyrate.

59 BASIC BIOLOGICAL SCIENCES↗

Techno-Economic Simulation Results Using dGeo for EGS-Based District Heating in the Northeastern United States

This dataset presents the results of techno-economic simulations performed using the Distributed Geothermal Market Demand Model (dGeo) to evaluate the feasibility of Enhanced Geothermal Systems (EGS)-based district heating in the Northeastern United States. Developed by the National Renewable Energy Laboratory (NREL), dGeo is a geospatially resolved, bottom-up modeling framework designed to explore the deployment potential of geothermal distributed energy resources. The dataset, created as part of the Cornell EGS Ground-Truthing Project, provides census tract-level data that includes inputs and outputs such as thermal demand, road length, energy prices, geothermal system sizing, annual energy contributions from geothermal and natural gas peaking boilers, system capital costs (CAPEX), operation and maintenance costs (OPEX), and the levelized cost of heat (LCOH). Key simulation parameters include geothermal gradients, measured well depths, production temperatures, and district heating piping lengths based on S1400 neighborhood road lengths. The simulations assume a target bottom hole temperature of 80C and the development of new district heating networks in each census tract.

15 GEOTHERMAL ENERGY↗

Microclimate observations associated with snowmelt experiment gradient sites, East River, Colorado, 2017 to 2020

The timing of snowmelt in mountain systems is a main driver of vegetation phenology and production, as well as recharge of soil moisture and ground water. Decreases in maximum snowpack and warmer spring temperatures have led to a higher frequency of early snowmelt. This study combines a natural elevation gradient with an experimental manipulation of snowmelt timing to assess the effect of earlier melt on hillslope and watershed ecohydrological processes. This data package includes csv files with plot and sub-plot level observations of soil temperature (SM_ST.csv), soil moisture (SM_SWC.csv), air temperature (SM_AirT.csv), and relative humidity (SM_rH.csv). The date range of this dataset includes a pre-treatment year (2017), two treatment years (2018 and 2019), and a post-treatment year (2020). Location IDs associated with this data package are: ER-LM, WG-UM, WG-LS, ER-US, and XX-AL. Related data packages include: “Colorado Elevation Gradient Snowmelt Manipulation Plant Phenology 2017-2018” and “Sensor-based phenology from snowmelt experiment gradient, East River, Colorado, 2017 to 2020”

54 ENVIRONMENTAL SCIENCES↗

Operational and Mission Highlights A Monthly Summary of Top Achievements

In September 2020, the Laboratory’s Chemical Microscopy Facility tripled in size, expanding to 1,000 square feet. Located at TA-46, Building158, this facility houses a unique Laboratory resource. Operated by the Physical Chemistry and Applied Spectroscopy (C-PCS) group, this facility was established to renew the Laboratory’s rich history in radiobiology. The facility combines biological research capabilities with isotope production, as well as radioanalytical and chemical infrastructure. This facility provides space for Biosafety Level 2 (BSL-2) research, with alpha-emitting and other radionuclides of interest associated with biological systems. Equipped with a Class ll/A2 biological safety cabinet, a radiological fume hood and a host of other analytical equipment, this facility serves an effective resource to evaluate the efficacy of targeted alpha therapeutics, as well as the deleterious effects of radiological materials on living organisms. The facility receives funding through LDRD- 20180005DR, “Establishing a Radiotherapeutic Capability to Counter Biothreats,” and the BES Heavy Element Chemistry Program at the Laboratory.

38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEA↗

Integrated Modeling Report for CO 2 Storage with Enhanced Oil Recovery in Northern Michigan

The Midwest Regional Carbon Sequestration Partnership (MRCSP) was founded in 2003 as part of the U.S. Department of Energy’s (DOE’s) Regional Carbon Sequestration Partnership initiative. Since its founding, MRCSP has made significant strides toward making CCUS a viable option for states in the region. The public/private consortium, funded through the DOE Regional Carbon Sequestration Initiative, brings together nearly 40 industry partners and 10 states. Battelle, as the project lead, oversees research, development and operations and coordinates activities among the partners. The incremental, phased approach has built a valuable knowledge base for the industry and paved the way for commercial-scale adoption of CCUS technologies. From 2008 to 2020, MRCSP Phase III focused on the development of large-scale injection projects. This report is part of a series of reports prepared under the Midwestern Regional Carbon Sequestration Partnership (MRCSP) Phase III (Development Phase). These reports summarize and detail the findings of the work conducted under the Phase III project. MRCSP has been investigating various reservoir characterization and modeling technologies related to Carbon Capture, Utilization, and Storage (CCUS) in conjunction with carbon dioxide enhanced oil recovery (CO 2 -EOR) operations in multiple Silurian-age (Niagaran), oil-bearing, carbonate pinnacle reefs in northern Michigan, USA. This report provides a comprehensive discussion of reservoir modeling studies that were conducted for tracking oil production, forecasting CO 2 plume migration, and estimating associated storage in a number of reefs that were at different stages of their CO 2 -EOR life cycle. The modeling process for simulating oil production, CO 2 injection, and associated storage in these reefs entails two phases. The first phase, geologic framework modeling, integrates all pertinent geological and geophysical data (from logs, cores and seismic surveys) about reservoir structure, geometry, rock types, and property distributions (porosity, permeability, water saturation) into a 3-D distributed grid-based static earth model (SEM). The second phase, dynamic reservoir modeling, uses the SEM as a platform to simulate the movement of oil, gas, water, and CO 2 within the reservoir during primary hydrocarbon production, as well as during subsequent phases such as CO 2 -injection assisted EOR, plume migration, and associated storage. In addition, an assessment of coupled process effects is also carried out, where the impacts of geochemical and geo-mechanical processed induced by CO 2 injection are studied.

01 COAL, LIGNITE, AND PEAT↗

High Temperature Steam Electrolysis Process Performance and Cost Estimates

Technology readiness levels (TRLs) of electrolysis systems have dramatically increased in recent years as the interest in clean hydrogen production and decarbonization of transportation, industrial and other sectors increases across the globe. This is especially true of high temperature steam electrolysis (HTSE) / solid oxide electrolysis cell (SOEC) systems which show promise of much higher system efficiencies than other more developed electrolysis technologies. This possibility of higher efficiencies of HTSE / SOEC systems has been previously assumed to be theoretically possible but in recent years it has become less theoretical and more realistic as an increasing amount of suppliers complete lab and pilot tests showing very promising results. Research in the areas of manufacturing techniques, material selection, electrode and electrolyte compositions, and balance of plant size and integration continues at a fast pace as an increasing number of suppliers both internationally and domestically become involved. The advantages of HTSE become more pronounced when HTSE is coupled with nuclear power plants (NPPs). This is because thermal energy produced by the nuclear reactor can be used in a series of heat transfer loops and heat exchangers to vaporize HTSE feedwater, which drastically improves the economics of the process. Idaho National Laboratory (INL) has been very involved in the research and modeling of HTSE systems for a number of years, in collaboration with other national laboratories, academia, and industry stakeholders both on the hydrogen production as well as the hydrogen demand side. The modeling completed over the years on a large variety of projects has led to a wealth of knowledge at INL including in the area of the technoeconomic assessment (TEA) of HTSE systems. TEAs include process modeling of the HTSE systems to calculate system energy requirements and equipment sizing, followed by estimation of capital and operating costs to enable calculation of the levelized cost of hydrogen (LCOH). The TEA work performed has produced incremental improvements and tuning of the methods, assumptions, models, and results of the analyses as well as providing some opportunities for validating these results. The purpose of this document is to record the current baseline HTSE analyses led by INL to show the current status of assumptions and costs of these systems. Given the rapid development of this technology, the variety of suppliers entering the space, and the increasing attention government and industry are giving to such systems, this document may be updated on a periodic basis with updated analysis and assumptions. This document compiles various analyses results and approaches completed over a period of years into a single document to be used as a baseline going forward. It represents what the INL HTSE analysis group assumes to be the internal best estimate of the current operation, costs, and landscape of the HTSE industry state of the art capability for current SOEC technology in an Nth-of-a-Kind (NOAK) plant, which in this study is defined as existence of the manufacturing capacity to support previous deployment of N = 100 count of 25 MWe modular HTSE blocks (with modular equipment component cost reductions specified as following a 95% learning curve). That said, This is a public document and as such so no proprietary data was used or included in this report. There may be HTSE suppliers that have performance specifications, and cost estimates, and test data that differ from the analysis presented in this document. This document is meant to be a best conservative estimate of the technology and not an absolute reference.

08 HYDROGEN↗

14 MeV Irradiation and Analysis of a 93% 239 Pu Target in Preparation for a F2019 FY22 Pu Campaign

In this work, we present the irradiation of a 93% 239 Pu with 14 MeV neutrons and subsequent analysis of the fission and activation products. The fully assembled target, a Pu metal bead encapsulated in Al, further encapsulated in welded stainless steel, was analyzed 22 times over more than 100 days using gamma emission analysis (GEA). Using the results from these analyses, R-values and fission yields for fission products were determined. To prepare for the FY22 Pu irradiation using the GODIVA critical assembly at NCERC, the irradiated Pu target was disassembled, dissolved, and separated using chemistry provided by LANL collaborators. This chemical separation was intended to remove the Pu from solution with little to no effect on the remaining elements. The chemistry was assessed to try to determine possible routes of fractionation of the sample and the fission products. The separation process used was successful for the bulk of the analyzed fission and activation products, as well as the added radiotracers. The final dissolved solution and separated fractions were analyzed by GEA looking at the remaining fission, activation and radiotracers and thermal ionization mass spectrometry analysis looking at the Pu isotopics.

14MeV↗