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At least 289 records · Page 16

Personal Computer (PC) based image processing applied to fluid mechanics

A PC based image processing system was employed to determine the instantaneous velocity field of a two-dimensional unsteady flow. The flow was visualized using a suspension of seeding particles in water, and a laser sheet for illumination. With a finite time exposure, the particle motion was captured on a photograph as a pattern of streaks. The streak pattern was digitized and processed using various imaging operations, including contrast manipulation, noise cleaning, filtering, statistical differencing, and thresholding. Information concerning the velocity was extracted from the enhanced image by measuring the length and orientation of the individual streaks. The fluid velocities deduced from the randomly distributed particle streaks were interpolated to obtain velocities at uniform grid points. For the interpolation a simple convolution technique with an adaptive Gaussian window was used. The results are compared with a numerical prediction by a Navier-Stokes computation.

Cho, Y.-C.↗

Personal computer (PC) based image processing applied to fluid mechanics research

A PC based image processing system was employed to determine the instantaneous velocity field of a two-dimensional unsteady flow. The flow was visualized using a suspension of seeding particles in water, and a laser sheet for illumination. With a finite time exposure, the particle motion was captured on a photograph as a pattern of streaks. The streak pattern was digitized and processsed using various imaging operations, including contrast manipulation, noise cleaning, filtering, statistical differencing, and thresholding. Information concerning the velocity was extracted from the enhanced image by measuring the length and orientation of the individual streaks. The fluid velocities deduced from the randomly distributed particle streaks were interpolated to obtain velocities at uniform grid points. For the interpolation a simple convolution technique with an adaptive Gaussian window was used. The results are compared with a numerical prediction by a Navier-Stokes commputation.

Cho, Y.-C.↗

An XMM Study of Low-Activity Coronal Stars of 1-2 Solar Masses

The objective of these observations was to take advantage of the large collecting area and sensitivity of the European Space Agency's XMM-Newton telescope to obtain X ray grating spectra of two relatively inactive late type stars, Beta Geminorun (K0 III) and the solar analog Beta Hydri (G2 IV). The observations were carried out as scheduled, achieving exposure times of 36 kiloseconds for Beta Gem and 27 kiloseconds for Beta Hyi. Unfortunately both stars were too faint to yield spectra with enough signal-to-noise for any further analysis. Consequently, there are no scientific results to report. The following pages illustrate the very weak X ray spectra that were obtained with the two RGS grating instruments for each star, first Beta Gem and then beta Hyi.

Simon, Theodore↗

Influence of Mild Chronic Stress and Social Isolation on Acute Ozone-Induced Alterations in Stress Biomarkers and Brain-Region-Specific Gene Expression in Male Wistar–Kyoto Rats

Individuals with psychosocial stress often experience an exaggerated response to air pollutants. Ozone (O 3 ) exposure has been associated with the activation of the neuroendocrine stress-response system. We hypothesized that preexistent mild chronic stress plus social isolation (CS), or social isolation (SI) alone, would exacerbate the acute effects of O 3 exposure on the circulating adrenal-derived stress hormones, and the expression of the genes regulating glucocorticoid stress signaling via an altered stress adaptation in a brain-region-specific manner. Male Wistar–Kyoto rats (5 weeks old) were socially isolated, plus were subjected to either CS (noise, confinement, fear, uncomfortable living, hectic activity, and single housing), SI (single housing only, restricted handling and no enrichment) or no stress (NS; double housing, frequent handling and enrichment provided) for 8 weeks. The rats were then exposed to either air or O 3 (0.8 ppm for 4 h), and the samples were collected immediately after. The indicators of sympathetic and hypothalamic–pituitary axis (HPA) activation (i.e., epinephrine, corticosterone, and lymphopenia) increased with O 3 exposure, but there were no effects from CS or SI, except for the depletion of serum BDNF. CS and SI revealed small changes in brain-region-specific glucocorticoid-signaling-associated markers of gene expression in the air-exposed rats (hypothalamic Nr3c1, Nr3c2 Hsp90aa1, Hspa4 and Cnr1 inhibition in SI; hippocampal HSP90aa1 increase in SI; and inhibition of the bed nucleus of the stria terminalis (BNST) Cnr1 in CS). Gene expression across all brain regions was altered by O 3 , reflective of glucocorticoid signaling effects, such as Fkbp5 in NS, CS and SI. The SI effects on Fkbp5 were greatest for SI in BNST. O 3 increased Cnr2 expression in the hypothalamus and olfactory bulbs of the NS and SI groups. O 3 , in all stress conditions, generally inhibited the expression of Nr3c1 in all brain regions, Nr3c2 in the hippocampus and hypothalamus and Bdnf in the hippocampus. SI, in general, showed slightly greater O 3 -induced changes when compared to NS and CS. Serum metabolomics revealed increased sphingomyelins in the air-exposed SI and O 3 -exposed NS, with underlying SI dampening some of the O 3 -induced changes. These results suggest a potential link between preexistent SI and acute O 3 -induced increases in the circulating adrenal-derived stress hormones and brain-region-specific gene expression changes in glucocorticoid signaling, which may partly underlie the stress dynamic in those with long-term SI.

gene expression↗

Confirmation of the spectral excess in DAMIC at SNOLAB with skipper CCDs

Here, we present results from a 3.25 kg–day target exposure of two silicon charge-coupled devices (CCDs), each with 24 megapixels and skipper readout, deployed in the DAMIC setup at SNOLAB. With a reduction in pixel readout noise of a factor of 10 relative to the previous detector, we investigate the excess population of low-energy events in the CCD bulk previously observed above expected backgrounds. We address the dominant systematic uncertainty of the previous analysis through a depth fiducialization designed to reject surface backgrounds on the CCDs. The measured bulk ionization spectrum confirms the presence of an excess population of low-energy events in the CCD target with characteristic rate of ~7 events per kg-day and electron-equivalent energies of ~80 eV, whose origin remains unknown.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗

The point spread function of the soft X-ray telescope aboard Yohkoh

The point spread function of the SXT telescope aboard Yohkoh has been measured in flight configuration in three different X-ray lines at White Sands Missile Range. We have fitted these data with an elliptical generalization of the Moffat function. Our fitting method consists of chi squared minimization in Fourier space, especially designed for matching of sharply peaked functions. We find excellent fits with a reduced chi squared of order unity or less for single exposure point spread functions over most of the CCD. Near the edges of the CCD the fits are less accurate due to vignetting. From fitting results with summation of multiple exposures we find a systematic error in the fitting function of the order of 3% near the peak of the point spread function, which is close to the photon noise for typical SXT images in orbit. We find that the full width to half maximum and fitting parameters vary significantly with CCD location. However, we also find that point spread functions measured at the same location are consistent to one another within the limit determined by photon noise. A 'best' analytical fit to the PSF as function of position on the CCD is derived for use in SXT image enhancemnent routines. As an aside result we have found that SXT can determine the location of point sources to about a quarter of a 2.54 arc sec pixel.

Martens, Petrus C.↗

Evaluation of Fairchild's Gate Drive Optocoupler, Type FOD3150, Under Wide Temperature Operation

An optocoupler is a semiconductor device that is used to transfer a signal between different parts of a circuit that need to be electrically isolated from one another - for example, where a high voltage is to be switched with a low voltage control signal. Optocouplers often can be used in place of relays. These optocouplers utilize an infrared LED (light emitting diode) and a photodetector such as a silicon controlled rectifier or photosensitive silicon diode for the transfer of the electronic signal between components of a circuit by means of a short optical transmission channel. For maximum coupling, the wave-length responses of the LED and the detector should be very similar. In switch-mode power supply applications, optocouplers offer advantages over transformers by virtue of simpler circuit design, reduced weight, and DC coupling capability. The effects of extreme temperature exposure and thermal cycling on the performance of a commercial-off-the-shelf (COTS) optocoupler, Fairchild FOD3150, were evaluated in this work. This 1.0 A output current, high noise immunity gate drive optocoupler utilizes an aluminum gallium arsenide (AlGaAs) LED, is capable of driving most 800V/20A IGBT/MOSFETs, and is suited for fast switching in motor control inverter applications and high performance power systems. Some of the specifications of the isolator chip are listed. The device was evaluated in terms of output response, output rise (t(sub r)) and fall times (t(sub f)), and propagation delays (using a 50% level between input and output during low to high (t(sub PLH)) and high to low (t(sub PLH)) transitions). The output supply current was also obtained. These parameters were recorded at various test temperatures between -190 C and +110 C.

Patterson, Richard↗

The Feasibility of Tropospheric and Total Ozone Determination Using a Fabry-perot Interferometer as a Satellite-based Nadir-viewing Atmospheric Sensor

Monitoring of the global distribution of tropospheric ozone (O3) is desirable for enhanced scientific understanding as well as to potentially lessen the ill-health impacts associated with exposure to elevated concentrations in the lower atmosphere. Such a capability can be achieved using a satellite-based device making high spectral resolution measurements with high signal-to-noise ratios; this would enable observation in the pressure-broadened wings of strong O3 lines while minimizing the impact of undesirable signal contributions associated with, for example, the terrestrial surface, interfering species, and clouds. The Fabry-Perot Interferometer (FPI) provides high spectral resolution and high throughput capabilities that are essential for this measurement task. Through proper selection of channel spectral regions, the FPI optimized for tropospheric O3 measurements can simultaneously observe a stratospheric component and thus the total O3 column abundance. Decreasing stratospheric O3 concentrations may lead to an increase in biologically harmful solar ultraviolet radiation reaching the earth's surface, which is detrimental to health. In this research, a conceptual instrument design to achieve the desired measurement has been formulated. This involves a double-etalon fixed-gap series configuration FPI along with an ultra-narrow bandpass filter to achieve single-order operation with an overall spectral resolution of approximately .068 cm(exp -1). A spectral region of about 1 cm(exp -1) wide centered at 1054.73 cm(exp -1) within the strong 9.6 micron ozone infrared band is sampled with 24 spectral channels. Other design characteristics include operation from a nadir-viewing satellite configuration utilizing a 9 inch (diameter) telescope and achieving horizontal spatial resolution with a 50 km nadir footprint. A retrieval technique has been implemented and is demonstrated for a tropical atmosphere possessing enhanced tropospheric ozone amounts. An error analysis assessing the impact on retrieved O3 amounts of the most significant uncertainties associated with this particular measurement has been performed for several different types of atmospheres. Results show the proposed instrumentation to enable a good measurement of absolute ozone amounts and an even better determination of relative changes, with a range of accuracy to within 7.55 to 20.6 percent for integrated tropospheric amounts (and 1.99 to 4.02 percent for total O3 column abundance) and a corresponding range in precision to within 7.73 to 10.4 percent (and 3.30 to 3.95 percent for total O3 column abundance), for the atmospheric conditions considered.

Larar, Allen Maurice↗

Macromolecular Topography Leaps into the Digital Age

A low-cost, real-time digital topography system is under development which will replace x-ray film and nuclear emulsion plates. The imaging system is based on an inexpensive surveillance camera that offers a 1000x1000 array of 8 im square pixels, anti-blooming circuitry, and very quick read out. Currently, the system directly converts x-rays to an image with no phosphor. The system is small and light and can be easily adapted to work with other crystallographic equipment. Preliminary images have been acquired of cubic insulin at the NSLS x26c beam line. NSLS x26c was configured for unfocused monochromatic radiation. Six reflections were collected with stills spaced from 0.002 to 0.001 degrees apart across the entire oscillation range that the reflections were in diffracting condition. All of the reflections were rotated to the vertical to reduce Lorentz and beam related effects. This particular CCD is designed for short exposure applications (much less than 1 sec) and so has a relatively high dark current leading to noisy raw images. The images are processed to remove background and other system noise with a multi-step approach including the use of wavelets, histogram, and mean window filtering. After processing, animations were constructed with the corresponding reflection profile to show the diffraction of the crystal volume vs. the oscillation angle as well as composite images showing the parts of the crystal with the strongest diffraction for each reflection. The final goal is to correlate features seen in reflection profiles captured with fine phi slicing to those seen in the topography images. With this development macromolecular topography finally comes into the digital age.

Lovelace, J.↗

Performance Characterization of UV Science Cameras Developed for the Chromospheric Lyman-Alpha Spectro-Polarimeter (CLASP)

The NASA Marshall Space Flight Center (MSFC) has developed a science camera suitable for sub-orbital missions for observations in the UV, EUV and soft X-ray. Six cameras will be built and tested for flight with the Chromospheric Lyman-Alpha Spectro-Polarimeter (CLASP), a joint National Astronomical Observatory of Japan (NAOJ) and MSFC sounding rocket mission. The goal of the CLASP mission is to observe the scattering polarization in Lyman-alpha and to detect the Hanle effect in the line core. Due to the nature of Lyman-alpha polarization in the chromosphere, strict measurement sensitivity requirements are imposed on the CLASP polarimeter and spectrograph systems; science requirements for polarization measurements of Q/I and U/I are 0.1% in the line core. CLASP is a dual-beam spectro-polarimeter, which uses a continuously rotating waveplate as a polarization modulator, while the waveplate motor driver outputs trigger pulses to synchronize the exposures. The CCDs are operated in frame-transfer mode; the trigger pulse initiates the frame transfer, effectively ending the ongoing exposure and starting the next. The strict requirement of 0.1% polarization accuracy is met by using frame-transfer cameras to maximize the duty cycle in order to minimize photon noise. Coating the e2v CCD57-10 512x512 detectors with Lumogen-E coating allows for a relatively high (30%) quantum efficiency at the Lyman-$\alpha$ line. The CLASP cameras were designed to operate with ≤10 e- /pixel/second dark current, ≤ 25 e- read noise, a gain of 2.0 and ≤0.1% residual non-linearity. We present the results of the performance characterization study performed on the CLASP prototype camera; dark current, read noise, camera gain and residual non-linearity.

Champey, Patrick↗

Thermal Design Challenges Posed by the Four Bed CO2 Scrubber COTS Air-Save Pump

The Four Bed Carbon Dioxide (4BCO2) scrubber Air-Save Pump (ASP) operates as part of the adsorbent bed regeneration cycle. The ASP removes residual air from the bed for return to the cabin prior to heat and vacuum exposure which drives out the CO2 regenerating the bed. 4BCO2 employs a Commercial Off-the-Shelf (COTS) scroll type air pump, repackaged in an acoustically insulated enclosure to reduce noise and mounted to a cold plate. The International Space Station (ISS) Low Temperature Loop (LTL) flow (operating between 38°F and 50°F) first cools the process air, and then flows through the cold plate, cooling the pump. This results in competing ASP thermal design goals: (1) to keep the pump and motor sufficiently cool and (2) to avoid forming condensation due to over-cooling. Surfaces below 60°F typically warrant careful consideration of condensation. A test-calibrated thermal model demonstrates such a balanced design is feasible with temperatures above 60°F. A separate, coupled fluid model predicts the potential for condensation formation, allowing risk assessment of flying with the unmodified design.

Daniel P Popok↗

Impulse Noise Sampling at 9965 - 300-pound energetic experimant

On October 1, 2022, sound level measurements were taken at various locations throughout Kirtland Air Force Base (KAFB) and Southeastern Albuquerque. The purpose was to support sound propagation modeling predictions and sound regulations for public exposure during the detonation of an approximately 300-pound energetic experiment. Ground Zero was located on Range 7 of Sandia Thunder Range (06647). A total of 8 measurement locations were identified (e.g., 5 on KAFB and 3 in the Southeastern Albuquerque neighborhoods).

42 ENGINEERING↗

Dynamic Transcriptomic and Phosphoproteomic Analysis During Cell Wall Stress in Aspergillus nidulans

The fungal cell-wall integrity signaling (CWIS) pathway regulates cellular response to environmental stress to enable wall repair and resumption of normal growth. This complex, interconnected, pathway has been only partially characterized in filamentous fungi. To better understand the dynamic cellular response to wall perturbation, a β-glucan synthase inhibitor (micafungin) was added to a growing A. nidulans shake-flask culture. From this flask, transcriptomic and phosphoproteomic data were acquired over 10 and 120 min, respectively. To differentiate statistically-significant dynamic behavior from noise, a multivariate adaptive regression splines (MARS) model was applied to both data sets. Over 1800 genes were dynamically expressed and over 700 phosphorylation sites had changing phosphorylation levels upon micafungin exposure. Twelve kinases had altered phosphorylation and phenotypic profiling of all non-essential kinase deletion mutants revealed putative connections between PrkA, Hk-8–4, and Stk19 and the CWIS pathway. Our collective data implicate actin regulation, endocytosis, and septum formation as critical cellular processes responding to activation of the CWIS pathway, and connections between CWIS and calcium, HOG, and SIN signaling pathways.

59 BASIC BIOLOGICAL SCIENCES↗

Performance Characterization of UV Science Cameras Developed for the Chromospheric Lyman-Alpha Spectro-Polarimeter

The NASA Marshall Space Flight Center (MSFC) has developed a science camera suitable for sub-orbital missions for observations in the UV, EUV and soft X-ray. Six cameras will be built and tested for flight with the Chromospheric Lyman-Alpha Spectro-Polarimeter (CLASP), a joint National Astronomical Observatory of Japan (NAOJ) and MSFC sounding rocket mission. The goal of the CLASP mission is to observe the scattering polarization in Lyman-alpha and to detect the Hanle effect in the line core. Due to the nature of Lyman-alpha polarization in the chromosphere, strict measurement sensitivity requirements are imposed on the CLASP polarimeter and spectrograph systems; science requirements for polarization measurements of Q/I and U/I are 0.1 percent in the line core. CLASP is a dual-beam spectro- polarimeter, which uses a continuously rotating waveplate as a polarization modulator, while the waveplate motor driver outputs trigger pulses to synchronize the exposures. The CCDs are operated in frame-transfer mode; the trigger pulse initiates the frame transfer, effectively ending the ongoing exposure and starting the next. The strict requirement of 0.1 percent polarization accuracy is met by using frame-transfer cameras to maximize the duty cycle in order to minimize photon noise. Coating the e2v CCD57-10 512x512 detectors with Lumogen-E coating allows for a relatively high (30 percent) quantum efficiency at the Lyman-alpha line. The CLASP cameras were designed to operate with a gain of 2.0 +/- 0.5, less than or equal to 25 e- readout noise, less than or equal to 10 e-/second/pixel dark current, and less than 0.1percent residual non-linearity. We present the results of the performance characterization study performed on the CLASP prototype camera; system gain, dark current, read noise, and residual non-linearity.

Champey, Patrick↗

Performance Characterization of UV Science Cameras Developed for the Chromospheric Lyman-Alpha Spectro-Polarimeter

The NASA Marshall Space Flight Center (MSFC) has developed a science camera suitable for sub-orbital missions for observations in the UV, EUV and soft X-ray. Six cameras will be built and tested for flight with the Chromospheric Lyman-Alpha Spectro-Polarimeter (CLASP), a joint National Astronomical Observatory of Japan (NAOJ) and MSFC sounding rocket mission. The goal of the CLASP mission is to observe the scattering polarization in Lyman-alpha and to detect the Hanle effect in the line core. Due to the nature of Lyman-alpha polarization in the chromosphere, strict measurement sensitivity requirements are imposed on the CLASP polarimeter and spectrograph systems; science requirements for polarization measurements of Q/I and U/I are 0.1 percent in the line core. CLASP is a dual-beam spectro-polarimeter, which uses a continuously rotating waveplate as a polarization modulator, while the waveplate motor driver outputs trigger pulses to synchronize the exposures. The CCDs are operated in frame-transfer mode; the trigger pulse initiates the frame transfer, effectively ending the ongoing exposure and starting the next. The strict requirement of 0.1 percent polarization accuracy is met by using frame-transfer cameras to maximize the duty cycle in order to minimize photon noise. Coating the e2v CCD57-10 512x512 detectors with Lumogen-E coating allows for a relatively high (30 percent) quantum efficiency at the Lyman-alpha line. The CLASP cameras were designed to operate with 10 e-/pixel/second dark current, 25 e- read noise, a gain of 2.0 +/- 0.5 and 1.0 percent residual non-linearity. We present the results of the performance characterization study performed on the CLASP prototype camera; dark current, read noise, camera gain and residual non-linearity.

Champey, P.↗

Acoustic sensor engineering evaluation test report

Two types of one-inch diameter sound pressure level sensors, which are candidates for monitoring ambient noise in the shuttle orbiter crew compartment during rest periods, were exposed to temperature, passive humidity, and vibration. One unexposed sensor of each type served as a reference unit. Except for the humidity exposures, each of the three capacitive microphones was individually tested in sequence with the essential voltage power supply and preamplifier. One unit exibited anomalous characteristics after the humidity exposure but returned to normal after being dried in an oven at 115 deg for two hours. Except for the humidity exposures, each of the three piezoelectric microphones was individually tested with a laboratory type amplifier. Two apparent failures occurred during these tests. The diaphragm on one was found ruptured after the fourth cycle of the humidity test. A second sensor showed an anomaly after the random vibration tests at which time its sensitivity was consistent at about one-half its former value.

Phillips, E. L., Jr.↗

Spectral anomalies in low dispersion SWP images

Physical interpretation of IUE spectra obtained with the SWP camera may be significantly affected by artificial spectral features of several types. In low dispersion large aperture SWP exposures of sources with nominally featureless spectra, a spectral imprint which alters the shape of the continuum, several spurious "emission features" which recur at fixed locations on the camera target, and fixed pattern noise which can result in illusory emission and absorption features were identified. The anomalies appear in spectra extracted from line by line files generated by IUESIPS at all epochs, regardless of the ITF or rectification scheme used for processing.

Hackney, R. L.↗

Detection of p-mode Oscillations in HD 35833 with NEID and TESS

We report the results of observations of p-mode oscillations in the G0 subgiant star HD 35833 in both radial velocities and photometry with NEID and TESS, respectively. We achieve separate, robust detections of the oscillation signal with both instruments (radial velocity amplitude ARV = 1.11 ± 0.09 m s−1, photometric amplitude Aphot = 6.42 ± 0.60 ppm, frequency of maximum power nmax = 595.71  17.28 μHz, and mode spacing Δν = 36.65 ± 0.96 μHz) as well as a nondetection in a TESS sector concurrent with the NEID observations. These data shed light on our ability to mitigate the correlated noise impact of oscillations with radial velocities alone and on the robustness of commonly used asteroseismic scaling relations. The NEID data are used to validate models for the attenuation of oscillation signals for exposure times t max < n-1 , and we compare our results to predictions from theoretical scaling relations and find that the observed amplitudes are weaker than expected by >4σ, hinting at gaps in the underlying physical models.

Arvind F Gupta↗