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At least 289 records · Page 16

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DOE OSTI2023

Genomics of turions from the Greater Duckweed reveal its pathways for dormancy and re‐emergence strategy

Summary Over 15 families of aquatic plants are known to use a strategy of developmental switching upon environmental stress to produce dormant propagules called turions. However, few molecular details for turion biology have been elucidated due to the difficulties in isolating high‐quality nucleic acids from this tissue. We successfully developed a new protocol to isolate high‐quality transcripts and carried out RNA‐seq analysis of mature turions from the Greater Duckweed Spirodela polyrhiza . Comparison of turion transcriptomes to that of fronds, the actively growing leaf‐like tissue, were carried out. Bioinformatic analysis of high confidence, differentially expressed transcripts between frond and mature turion tissues revealed major pathways related to stress tolerance, starch and lipid metabolism, and dormancy that are mobilized to reprogram frond meristems for turion differentiation. We identified the key genes that are likely to drive starch and lipid accumulation during turion formation, as well as those in pathways for starch and lipid utilization upon turion germination. Comparison of genome‐wide cytosine methylation levels also revealed evidence for epigenetic changes in the formation of turion tissues. Similarities between turions and seeds provide evidence that key regulators for seed maturation and germination were retooled for their function in turion biology.

59 BASIC BIOLOGICAL SCIENCES↗

NASA NTRS1985

Propagation of quasifracture in viscoelastic media under low-cycle repeated stressing

The propagation of a craze as quasifracture under repeated cyclic stressing in polymeric systems has been under intensive investigation recently. Based upon a time-dependent crazing theory, the governing differential equation describing the propagation of a single craze as quasifracture in an infinite viscoelastic plate has been solved for sinusoidal stresses. Numerical methods have been employed to obtain the normalized craze length as a function of time. The computed results indicate that the length of a quasifracture may decelerate and decrease indicating that its velocity can reverse. This behavior may be consistent with the observed and much discussed craze healing and the enclosure model in fatigue and fracture of solids.

Liu, X. P.↗

DOE OSTI2026

Novel Cell-Type-Specific Drought-Responsive Proteins in Root Tips of Field-Grown Perennial Switchgrass

The root-tip region of plants, including the root cap, forms the most basal terminal of the root and exhibits a high degree of cellular complexity in terms of morphology, cytological function, and interaction with environmental cues in the soil. Cells in this region follow a developmental trajectory, transitioning from stem cells to meristematic cells, and ultimately to fully differentiated cell types. However, our understanding of root-tip cell-type specific proteomic responses to abiotic stresses, such as drought, particularly under field conditions, remains limited. This study aimed to identify spatially resolved, cell type-specific proteomes in switchgrass (Panicum virgatum) root tips under drought stress. Root tips were collected from seven-year-old, field-grown switchgrass ‘Alamo’ plants excavated under both well-watered and long-term drought conditions. Cell type-specific proteins were identified using laser capture microdissection (LCM) coupled with nanoPOTS (Nanodroplet Processing in One Pot for Trace Samples) and nano-LC-MS proteomics analysis. Five distinct cell types were targeted: (1) cells in the quiescent center and stem cell niche (QuC), (2) protodermal epidermal cells (PEC) in the meristematic zone, (3) epidermal cells in the transition and elongation zones above the root cap (Epi), (4) peripheral root cap cells (PRC), forming 2–3 layers below the PEC and 1–2 layers above the root border cells, and (5) columella root cap cells (Col) comprising of the columella initials and a single underlying layer of cells undergoing active growth. Principal component analysis (PCA) revealed clear separation among the five targeted cell types, confirming distinct proteomic profiles. Proteins predominantly enriched in each cell type were linked to distinct cellular functions, with QuC cells showing involvement in chromosomal behavior, DNA replication, and mitosis—key processes for stem cell niche regulation. Drought stress resulted in alterations of proteostasis, as evidenced by significant decreases in ribosomal proteins and increases in protein synthesis inhibitors. Moreover, drought stress induced unique cell-type–specific proteins involved in phytohormone biosynthesis and signaling pathways, including auxin, cytokinin, and jasmonic acid. In particular, QuC cells were more highly enriched in proteins associated with DNA repair and mitotic processes. Metabolic pathways related to amino acids, carbohydrates, and lipids were differentially affected in a cell-type–dependent manner, whereas general stress-responsive proteins exhibited consistent changes across all five cell types. Overall, this study provides unique spatially resolved, cell-type-specific proteomic profiles in root tips, representing a significant advancement in our understanding of the cellular mechanisms underlying plant responses to drought stress in natural field conditions.

perennial grass↗

NASA NTRS1992

Morphologic differentiation of colon carcinoma cell lines HT-29 and HT-29KM in rotating-wall vessels

A new low shear stress microcarrier culture system has been developed at NASA's Johnson Space Center that permits three-dimensional tissue culture. Two established human colon adenocarcinoma cell lines, HT-29, an undifferentiated, and HT-29KM, a stable, moderately differentiated subline of HT-29, were grown in new tissue culture bioreactors called Rotating-Wall Vessels (RWVs). RWVs are used in conjunction with multicellular cocultivation to develop a unique in vitro tissue modeling system. Cells were cultivated on Cytodex-3 microcarrier beads, with and without mixed normal human colonic fibroblasts, which served as the mesenchymal layer. Culture of the tumor lines in the absence of fibroblasts produced spheroidlike growth and minimal differentiation. In contrast, when tumor lines were co-cultivated with normal colonic fibroblasts, initial growth was confined to the fibroblast population until the microcarriers were covered. The tumor cells then commenced proliferation at an accelerated rate, organizing themselves into three-dimensional tissue masses that achieved 1.0- to 1.5-cm diameters. The masses displayed glandular structures, apical and internal glandular microvilli, tight intercellular junctions, desmosomes, cellular polarity, sinusoid development, internalized mucin, and structural organization akin to normal colon crypt development. Differentiated samples were subjected to transmission and scanning electron microscopy and histologic analysis, revealing embryoniclike mesenchymal cells lining the areas around the growth matrices. Necrosis was minimal throughout the tissue masses. These data suggest that the RWV affords a new model for investigation and isolation of growth, regulatory, and structural processes within neoplastic and normal tissue.

Colonic Neoplasms/pathology↗

NASA NTRS1992

Free-edge effects in laminates under extension, bending and twisting. II - Sublaminate/layer modeling and analysis

The stress-function-based variational method of Yin (1991) is extended and modified into a combined layer/sublaminate approach applicable to a laminated strip composed of a large number of differently orientated, anisotropic elastic plies. Lekhnitskii's (1963) stress functions are introduced into two interior layers adjacent to a particular interface. The remaining layers are grouped into an upper sublaminate and a lower sublaminate. The stress functions are expanded in truncated power series of the thickness coordinate, and the differential equations governing the coefficient functions are derived by using the complementary virtual work principle. The layer/sublaminate approach limits the dimension of the eigenvalue problem to a fixed number irrespective of the number of layers in the sublaminate, so that reasonably accurate solutions of the interlaminar stresses can be computed with extreme ease. For symmetric, four-layer, angle-ply and cross-ply laminates, a comparison of the previous analysis results based on the pure layer model and new results based on two different layer/sublaminate models indicates reasonable over-all agreement in the interlaminar stresses and superior agreement in the total peeling and shearing force.

Yin, Wan-Lee↗

NASA NTRS1973

Three-dimensional elastic stress and displacement analysis of finite circular geometry solids containing cracks

A seminumerical method is presented for solving a set of coupled partial differential equations subject to mixed and coupled boundary conditions. The use of this method is illustrated by obtaining solutions for two circular geometry and mixed boundary value problems in three-dimensional elasticity. Stress and displacement distributions are calculated in an axisymmetric, circular bar of finite dimensions containing a penny-shaped crack. Approximate results for an annular plate containing internal surface cracks are also presented.

Gyekenyesi, J. P.↗

DOE OSTI2024

Diverse fates of ancient horizontal gene transfers in extremophilic red algae

Abstract Horizontal genetic transfer (HGT) is a common phenomenon in eukaryotic genomes. However, the mechanisms by which HGT‐derived genes persist and integrate into other pathways remain unclear. This topic is of significant interest because, over time, the stressors that initially favoured the fixation of HGT may diminish or disappear. Despite this, the foreign genes may continue to exist if they become part of a broader stress response or other pathways. The conventional model suggests that the acquisition of HGT equates to adaptation. However, this model may evolve into more complex interactions between gene products, a concept we refer to as the ‘Integrated HGT Model’ (IHM). To explore this concept further, we studied specialized HGT‐derived genes that encode heavy metal detoxification functions. The recruitment of these genes into other pathways could provide clear examples of IHM. In our study, we exposed two anciently diverged species of polyextremophilic red algae from the Galdieria genus to arsenic and mercury stress in laboratory cultures. We then analysed the transcriptome data using differential and coexpression analysis. Our findings revealed that mercury detoxification follows a ‘one gene‐one function’ model, resulting in an indivisible response. In contrast, the ars H gene in the arsenite response pathway demonstrated a complex pattern of duplication, divergence and potential neofunctionalization, consistent with the IHM. Our research sheds light on the fate and integration of ancient HGTs, providing a novel perspective on the ecology of extremophiles.

59 BASIC BIOLOGICAL SCIENCES↗

NASA NTRS1982

A viscous-inviscid interaction algorithm for three-dimensional turbulent subsonic aerodynamic juncture region flow

An order-of magnitude analysis of the subsonic, three-dimensional, steady time-averaged Navier-Stokes equations for semibounded aerodynamic juncture geometries yields the parabolic Navier-Stokes simplification. The numerical solution of the resultant pressure Poisson equation is cast into complementary and particular parts, yielding an iterative interaction algorithm with an exterior three-dimensional potential flow solution. A parabolic transverse momentum equation set is constructed, wherein robust enforcement first-order continuity effects is accomplished using a penalty differential constraint concept within a finite element solution algorithm. A Reynolds stress constitutive equation, with low turbulence Reynolds number wall functions, is employed for closure, using parabolic forms of the two-equation turbulent kinetic energy-dissipation equation system. The algorithm is employed to predict the three-dimensional turbulent flowfield in the juncture region formed by two intersecting parabolic arcs at a free-stream Mach-number of 0.08, and Re/C = 600,000/m.

Baker, A. J.↗

NASA NTRS1981

Thematic mapper - An overview of spectral band registration

The Thematic Mapper (TM) is a high-resolution radiometer designed for earth resources classification and mapping. The TM employs multispectral scanning in a near polar orbit to sweep a 185-km swath. Data are obtained through a combination of spacecraft motion and the sweeping action of the scan mirror. These data are transmitted either directly to ground stations around the world or through a relay to the central data processing facility at White Sands, NM. Seven spectral passbands are employed, and applications include coastal water mapping, soil vegetation differentiation, biomass surveys, water body delineation, vegetation moisture measurement, plant heat stress management, and hydrothermal mapping. Attention is given to the scan mirror assembly, scan nonlinearities, the characterization and compensation of scan profiles, experimental performance, and a procedure for midscan correction.

Freudenstein, W. H.↗

NASA NTRS1982

The influence of cobalt on the microstructure of the nickel-base superalloy MAR-M247

Nickel was substituted for Co to produce 0, 5, and the standard 10% versions of MAR-M247, a cast nickel-base superalloy. The microstructures of the alloys were examined in as-cast, heat treated, aged, and stress-rupture tested conditions using a variety of metallographic techniques and differential thermal analysis. As cobalt concentration was reduced from 10 to 0 wt %, the gamma-prime weight fraction decreased from 59 to 41%; W and Ti concentrations in the gamma-prime phase increased from 5 to 8 and 2 to 3 at.%, respectively; the mean gamma-prime particle size increased from 0.6 to 0.8 micron; Cr and Al concentrations in the gamma matrix decreased from 17 to 13 and 15 to 12 at.%, respectively; and the weight fraction of carbides increased by approximately 1%.

Nathal, M. V.↗

NASA NTRS1982

The CMC:3DPNS computer program for prediction of three-dimensional, subsonic, turbulent aerodynamic juncture region flow. Volume 1: Theoretical

An order-of-magnitude analysis of the subsonic three dimensional steady time averaged Navier-Stokes equations, for semibounded aerodynamic juncture geometries, yields the parabolic Navier-Stokes simplification. The numerical solution of the resultant pressure Poisson equation is cast into complementary and particular parts, yielding an iterative interaction algorithm with an exterior three dimensional potential flow solution. A parabolic transverse momentum equation set is constructed, wherein robust enforcement of first order continuity effects is accomplished using a penalty differential constraint concept within a finite element solution algorithm. A Reynolds stress constitutive equation, with low turbulence Reynolds number wall functions, is employed for closure, using parabolic forms of the two-equation turbulent kinetic energy-dissipation equation system. Numerical results document accuracy, convergence, and utility of the developed finite element algorithm, and the CMC:3DPNS computer code applied to an idealized wing-body juncture region. Additional results document accuracy aspects of the algorithm turbulence closure model.

Baker, A. J.↗

NASA NTRS1987

Damage growth in composite laminates with interleaves

The influence of placing interleaves between fiber reinforced plies in multilayered composite laminates is investigated. The geometry of the composite is idealized as two dimensional, isotropic, linearly elastic media made of a damaged layer bonded between two half planes and separated by thin interleaves of low extensional and shear moduli. The damage in the layer is taken in the form of a symmetric crack perpendicular to the interface and may extend up to the interface. The case of an H-shaped crack in the form of a broken layer with delamination along the interface is also analyzed. The interleaves are modeled as distributed shear and tension springs. Fourier integral transform techniques are used to develop solutions in terms of singular integral equations. An asymptotic analysis of the integral equations based on Muskhelishvili's techniques reveals logarithmically singular axial stresses in the half plane at the crack tips for the broken layer. For the H shaped crack, similar singularities are found to exist in the axial stresses at the interface crack tips in the layer and the half plane. The solution of the equations is found numerically for the stresses and displacements by using the Hadamard's concept of direct differentiation of Cauchy integrals as well as Gaussian integration techniques.

Goree, James G.↗

NASA NTRS1992

Exploiting similarity in turbulent shear flows for turbulence modeling

It is well known that current k-epsilon models cannot predict the flow over a flat plate and its wake. In an effort to address this issue and other issues associated with turbulence closure, a new approach for turbulence modeling is proposed which exploits similarities in the flow field. Thus, if we consider the flow over a flat plate and its wake, then in addition to taking advantage of the log-law region, we can exploit the fact that the flow becomes self-similar in the far wake. This latter behavior makes it possible to cast the governing equations as a set of total differential equations. Solutions of this set and comparison with measured shear stress and velocity profiles yields the desired set of model constants. Such a set is, in general, different from other sets of model constants. The rational for such an approach is that if we can correctly model the flow over a flat plate and its far wake, then we can have a better chance of predicting the behavior in between. It is to be noted that the approach does not appeal, in any way, to the decay of homogeneous turbulence. This is because the asymptotic behavior of the flow under consideration is not representative of the decay of homogeneous turbulence.

Robinson, David F.↗

NASA NTRS1939

Some notes on the numerical solution of shear-lag and mathematically related problems

The analysis of box beams with shear deformation of the flanges can be reduced to the solution of a differential equation. The same equation is met in other problems of stress analysis. No analytical solutions of this equation can be given for practical cases, and numerical methods of evaluation must be used. Available methods are briefly discussed. Two numerical examples show the application of the step-by-step method of integration to shear-lag problems.

Kuhn, Paul↗

NASA NTRS1999

Delayed translational silencing of ceruloplasmin transcript in gamma interferon-activated U937 monocytic cells: role of the 3' untranslated region

Ceruloplasmin (Cp) is an acute-phase protein with ferroxidase, amine oxidase, and pro- and antioxidant activities. The primary site of Cp synthesis in human adults is the liver, but it is also synthesized by cells of monocytic origin. We have shown that gamma interferon (IFN-gamma) induces the synthesis of Cp mRNA and protein in monocytic cells. We now report that the induced synthesis of Cp is terminated by a mechanism involving transcript-specific translational repression. Cp protein synthesis in U937 cells ceased after 16 h even in the presence of abundant Cp mRNA. RNA isolated from cells treated with IFN-gamma for 24 h exhibited a high in vitro translation rate, suggesting that the transcript was not defective. Ribosomal association of Cp mRNA was examined by sucrose centrifugation. When Cp synthesis was high, i.e., after 8 h of IFN-gamma treatment, Cp mRNA was primarily associated with polyribosomes. However, after 24 h, when Cp synthesis was low, Cp mRNA was primarily in the nonpolyribosomal fraction. Cytosolic extracts from cells treated with IFN-gamma for 24 h, but not for 8 h, contained a factor which blocked in vitro Cp translation. Inhibitor expression was cell type specific and present in extracts of human cells of myeloid origin, but not in several nonmyeloid cells. The inhibitory factor bound to the 3' untranslated region (3'-UTR) of Cp mRNA, as shown by restoration of in vitro translation by synthetic 3'-UTR added as a "decoy" and detection of a binding complex by RNA gel shift analysis. Deletion mapping of the Cp 3'-UTR indicated an internal 100-nucleotide region of the Cp 3'-UTR that was required for complex formation as well as for silencing of translation. Although transcript-specific translational control is common during development and differentiation and global translational control occurs during responses to cytokines and stress, to our knowledge, this is the first report of translational silencing of a specific transcript following cytokine activation.

NASA Discipline Regulatory Physiology↗

NASA NTRS2000

Altered cytokine production by specific human peripheral blood cell subsets immediately following space flight

In this study, flow cytometry was used to positively identify the specific lymphocyte subsets exhibiting space flight-induced alterations in cytokine production. Whole blood samples were collected from 27 astronauts at three points (one preflight, two postflight) surrounding four space shuttle missions. Assays performed included serum/urine stress hormones, white blood cell (WBC) phenotyping, and intracellular cytokine production following mitogenic stimulation. Absolute levels of peripheral granulocytes were significantly elevated following space flight, but the levels of circulating lymphocytes and monocytes were unchanged. Lymphocyte subset analysis demonstrated a decreased percentage of T cells, whereas percentages of B cells and natural killer (NK) cells remained unchanged after flight. Nearly all the astronauts exhibited an increased CD4/CD8 T cell ratio. Assessment of naive (CD45RA+) vs. memory (CD45RO+) CD4+ T cell subsets was ambiguous, and subjects tended to group within specific missions. Although no significant trend was seen in absolute monocyte levels, a significant decrease in the percentage of the CD14+ CD16+ monocytes was seen following space flight in all subjects tested. T cell (CD3+) production of interleukin-2 (IL-2) was significantly decreased after space flight, as was IL-2 production by both CD4+ and CD8+ T cell subsets. Production of interferon-gamma (IFN-gamma) was not altered by space flight for the CD8+ cell subset, but there was a significant decrease in IFN-gamma production for the CD4+ T cell subset. Serum and urine stress hormone analysis indicated significant physiologic stresses in astronauts following space flight. Altered peripheral leukocyte subsets, altered serum and urine stress hormone levels, and altered T cell cytokine secretion profiles were all observed postflight. In addition, there appeared to be differential susceptibility to space flight regarding cytokine secretion by T cell subsets. These alterations may be the result of either microgravity exposure or the physiologic stresses of landing and readaptation to unit gravity. Future studies, including in-flight analysis or sampling, will be necessary to determine the cause of these alterations.

NASA Discipline Cell Biology↗

NASA NTRS1977

Three-dimensional elastic stress and displacement analysis of finite geometry solids containing cracks

The line method of analysis is applied to the Navier-Cauchy equations of elastic equilibrium to calculate the displacement fields in finite geometry bars containing central, surface, and double-edge cracks under extensionally applied uniform loading. The application of this method to these equations leads to coupled sets of simultaneous ordinary differential equations whose solutions are obtained along sets of lines in a discretized region. Normal stresses and the stress intensity factor variation along the crack periphery are calculated using the obtained displacement field. The reported results demonstrate the usefulness of this method in calculating stress intensity factors for commonly encountered crack geometries in finite solids.

Kring, J.↗

NASA NTRS1977

Three-dimensional elastic stress and displacement analysis of finite geometry solids containing cracks

Kring, J.↗