SEARCH · Engineering Papers

Results for “SITE CHARACTERIZATION”

Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

SourceResearch connectionFrom yearThrough year

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 289 records · Page 16

All sources NASA NTRS DOE OSTI

NASA NTRS1999

Modem Characterization Through a Wideband, Hard-Limited Ka-Band Satellite Channel

NASA is using a commercial customized TDMA/FDMA bandwidth on demand modem for use with the Advanced Communications Technology Satellite (ACTS) to highlight the numerous services and experiments that can be performed using small Ka-Band terminals. Characterizing the modems proved challenging due to the characteristics of the satellite transponder. The ACTS channel is hard-limited and up to 900 MHz wide. The channel has some unusual dynamic properties due to the satellite and antenna system, which make modem testing through the satellite challenging and the test requirements stringent. The satellite Multi-Beam Antenna (MBA) has a 1 hertz oscillation induced by the momentum wheel, which causes the transmit antenna pattern to move slightly. This results in a 1 hertz oscillation in the ground station receive power, with amplitude changes up to 1 dB depending on terminal location within a spot beam and associated gain slope. In addition, ACTS experiences a solar induced "thermal event" each day. This "thermal event" occurs when the sun heats the antenna support structure causing the transmit and receive reflectors to mispoint. This results in a slowly decreasing or increasing power density at the ground station receiver as the antenna pattern moves off bore-site. This paper describes the method used to fully characterize the TDMA/FDMA modem through the ACTS wideband, hard-limited transponder. In particular, techniques are discussed for conducting RF measurements on such a channel, the affect that the thermal characteristics and 1 hertz variations have on the accuracy of the results, and suggested means to minimize the error and provide useful and valuable data.

Reinhart, Richard C.↗

NASA NTRS2010

Development of a NASA 2018 Mars Landed Mission Concept

Fundamental to NASA's Mars Exploration Program (MEP) is an ongoing development of an integrated and coordinated set of possible future candidate missions that meet fundamental science and programmatic objectives of NASA and the Mars scientific community. In the current planning horizon of the NASA MEP, a landed mobile surface exploration mission launching in the 2018 Mars launch opportunity exists as a candidate project to meet MEP in situ science and exploration objectives. This paper describes the proposed mission science objectives and the mission implementation concept developed for the 2018 opportunity. As currently envisioned, this mission concept seeks to explore a yet-to-be-selected site with high preservation potential for physical and chemical biosignatures, evaluate paleoenvironmental conditions, characterize the potential for preservation of biosignatures, and access multiple sequences of geological units in a search for evidence of past life and/or prebiotic chemistry at a site on Mars.

Mars Astrobiology Explorer- Cacher (MAX-C)↗

NASA NTRS2018

K-Means Cluster Study for Radiofrequency Propagation Characterization

The objective of this study is to design a simple method for mining radio frequency (RF) propagation data. The study explored the characteristics of a large dataset of propagation experiments conducted over the span of years and using several ground stations around the world. Furthermore, this study developed simple predictive models that can be used for link characterization and overall propagation behavior description, without the need for physical measurements on-site. It is understood that such statistical learning has several drawbacks in terms of accuracy and precision. K-means clustering was used to characterize the data set in a way never explored before in an attempt to create useful tools that reduce cost, time and risk. K-means clustering was used to characterize the data set. Cosine distance was used as a method to determine the optimal number for clustering each feature. Dependence and independence analysis was performed to explore intra and inter-sensitivity between the presented features, with respect to each other and time. Several predicative models were generated and evaluated with respect to a test set to assess a measure of prediction accuracy and precision. A simple method for data analysis was developed and tested as the basis for further studies and future refinement to produce optimal performing models.

Cognitive↗

NASA NTRSDate not supplied

Additional Characterization of Sonoran Pics in Support of A Stable Multi-Sensor Geostationary Imager Record

The Sonoran Desert is the most utilized Pseudo Invariant Calibration Site (PICS) located in the Americas for post-launch radiometer drift monitoring and sensor pair radiometric scaling. The Sonoran Desert is located near the US Arizona and Mexican border with an elevation of 37 m. The site has small spatial and angular variations; however, soil moisture can cause short- term periodic reflectance fluctuations. The NASA Clouds and the Earth’s Radiant Energy System (CERES) project utilizes the site to validate the GOES East and West imager calibration coefficients derived from inter-calibrating GOES with MODIS. Because the daily local noon angular solar and viewing angles repeat every year over the site, a daily exoatmospheric radiance model (DERM) can be constructed over the lifetime of a well-calibrated GOES sensor. DERM based on a well-calibrated GOES imager can then be used to determine the calibration coefficients of successive GOES imagers to provide a stable multi-sensor GOES imager record. This will ensure that the GOES retrieved clouds and derived broadband fluxes can be used to infer the regional diurnal cycle in between Terra and Aqua CERES measurements to accurately compute the daily mean regional fluxes and clouds over the 20-year CERES SYN1deg product. Although the Sonoran Desert surface reflectance and atmospheric column vary seasonally, the inter-annual variability of the seasonal cycle is small, but it adds noise to the resulting DERM calibration coefficients. We seek to reduce the uncertainty of the DERM approach by improving the clear-sky filtering and correlating the observed interannual reflectance variability with atmospheric parameters, such as precipitable water, ozone concentration, and aerosol optical depth. The additional characterization of the Sonoran Desert site DERM for both the GOES East and West positions should improve the accuracy of the CERES SYN1deg product clouds and fluxes.

Prathana Khakurel↗

NASA NTRS2017

Mars 2020 Site-Specific Mission Performance Analysis: Part 2. Surface Traversability

The Mars 2020 Rover Mission (M2020) is characterized by long-range traverses between scientific Regions Of Interest (ROIs), as well as the demanding requirement on the distance and time for the inter-ROI traverses. As a result, surface traversability is one of the major driving factors for the landing site selection of M2020. With the newly developed Mars Terrain Traversability analysis Tools (MTTT), we performed traversability analysis of the eight candidate landing sites with an unprecedented granularity. This paper describes the MTTT analysis capabilities, as well as how the MTTT capabilities were used to down-select from eight to three candidate landing sites for further evaluation.

Milkovich, Sarah↗

NASA NTRS2019

Identification of Absorbing Aerosol Types at a Site in the Northern Edge of Indo-Gangetic Plain and a Polluted Valley in the Foothills of the Central Himalayas

Identification of atmospheric aerosol types and characterization of absorbing aerosols, based on AErosol RObotic NETwork (AERONET) data collected during 2013–2014 over two sites in Nepal: Lumbini in the northernmost part of central Indo-Gangetic Plain (IGP) and Kathmandu Valley in foothills of the central Himalayas, have been conducted in the present study. The relationship between four aerosol parameters; Extinction Angstrom Exponent (EAE), Absorption Angstrom Exponent (AAE), Single Scattering Albedo (SSA) and Real Refractive Index (RRI) was analyzed to study the aerosol types. This resulted in the identification of two types of aerosols concerning their origin: biomass burning and urban/industrial mix. Furthermore, to understand the absorbing aerosol types, the relationship between aerosol size parameters; Fine Mode Fraction (FMF) and Angstrom Exponent (AE), and aerosol absorption characteristics; SSA and AAE were investigated. In regards to the absorbing aerosol types, ‘Mostly BC’ was the dominant absorbing aerosol, over both sites, with comparatively negligible contribution from other absorbing aerosol types such as dust. The aerosol subtypes obtained from satellite-borne CALIPSO instrument supported the results derived from the AERONET data. The CALIPSO images also indicated that the aerosols over the foothills of the Himalayas could extend to the height of >5 km above the ground, which could be transported towards the Himalayan and Tibetan Plateau (HTP) region with sensitive ecosystems. The multi-sites based study of long-term records is required to elucidate the nature and trends of aerosols in the HTP region and any perturbation to the atmospheric environment and other environments in this region.

Aerosol types↗

NASA NTRS2018

Results from the Deep Convective Clouds-Based Response Versus Scan-Angle Characterization for the MODIS Reflective Solar Bands

The Terra and Aqua Moderate-Resolution Imaging Spectroradiometer (MODIS) scan mirror reflectance is a function of the angle of incidence (AOI) and was characterized prior to launch by the instrument vendor. The relative change of the prelaunch response versus scan angle (RVS) is tracked and linearly scaled on-orbit using observations at two AOIs of 11.2° and 50.2° corresponding to the moon view and solar diffuser, respectively. As the missions continue to operate well beyond their design life of six years, the assumption of linear scaling between the two AOIs is known to be inadequate in accurately characterizing the RVS, particularly at short wavelengths. Consequently, an enhanced approach of supplementing the on-board measurements with response trends from desert pseudoinvariant calibration sites (PICS) was formulated in MODIS Collection 6 (C6). An underlying assumption for the continued effectiveness of this approach is the long-term (multiyear) and short-term (month to month) stability of the PICS. Previous work has shown that the deep convective clouds (DCC) can also be used to monitor the onorbit RVS performance with less trend uncertainties compared with desert sites. In this paper, the raw sensor response to the DCC is used to characterize the on-orbit RVS on a band and mirror-side basis. These DCC-based RVS results are compared with those of C6 PICS-based RVS, showing an agreement within 2% observed in most cases. The pros and cons of using a DCC-based RVS approach are also discussed in this paper. Although this reaffirms the efficacy of the C6 PICS-based RVS, the DCC-based RVS approach presents itself as an effective alternative for future considerations. Potential applications of this approach to other instruments, such as Suomi National Polarorbiting Partnership, Joint Polar Satellite Systems, and Visible Infrared Imaging Radiometer Suite, are also discussed.

Angal, Amit↗

NASA NTRS1972

Apollo 14 active seismic experiment.

Explosion seismic refraction data indicate that the lunar near-surface rocks at the Apollo 14 site consist of a regolith 8.5 meters thick and characterized by a compressional wave velocity of 104 meters per second. The regolith is underlain by a layer with a compressional wave velocity of 299 meters per second. The thickness of this layer, which we interpret to be the Fra Mauro Formation, is between 16 and 76 meters. The layer immediately beneath this has a velocity greater than 370 meters per second. We found no evidence of permafrost.

Watkins, J. S.↗

NASA NTRS1995

Global stratigraphy of Venus: Analysis of a random sample of thirty-six test areas

Basilevsky, Alexander T.↗

NASA NTRS1995

Global Stratigraphy of Venus: Analysis of a Random Sample of Thirty-Six Test Areas

The age relations between 36 impact craters with dark paraboloids and other geologic units and structures at these localities have been studied through photogeologic analysis of Magellan SAR images of the surface of Venus. Geologic settings in all 36 sites, about 1000 x 1000 km each, could be characterized using only 10 different terrain units and six types of structures. These units and structures form a major stratigraphic and geologic sequence (from oldest to youngest): (1) tessera terrain; (2) densely fractured terrains associated with coronae and in the form of remnants among plains; (3) fractured and ridged plains and ridge belts; (4) plains with wrinkle ridges; (5) ridges associated with coronae annulae and ridges of arachnoid annulae which are contemporary with wrinkle ridges of the ridged plains; (6) smooth and lobate plains; (7) fractures of coronae annulae, and fractures not related to coronae annulae, which disrupt ridged and smooth plains; (8) rift-associated fractures; and (9) craters with associated dark paraboloids, which represent the youngest 1O% of the Venus impact crater population (Campbell et al.), and are on top of all volcanic and tectonic units except the youngest episodes of rift-associated fracturing and volcanism; surficial streaks and patches are approximately contemporary with dark-paraboloid craters. Mapping of such units and structures in 36 randomly distributed large regions (each approximately 10(exp 6) sq km) shows evidence for a distinctive regional and global stratigraphic and geologic sequence. On the basis of this sequence we have developed a model that illustrates several major themes in the history of Venus. Most of the history of Venus (that of its first 80% or so) is not preserved in the surface geomorphological record. The major deformation associated with tessera formation in the period sometime between 0.5-1.0 b.y. ago (Ivanov and Basilevsky) is the earliest event detected. In the terminal stages of tessera fon-nation, extensive parallel linear graben swarms representing a change in the style of deformation from shortening to extension were formed on the tessera and on some volcanic plains that were emplaced just after, and perhaps also during the latter stages of the major compressional phase of tessera emplacement. Our stratigraphic analyses suggest that following tessera formation, extensive volcanic flooding resurfaced at least 85% of the planet in the form of the presently-ridged and fractured plains. Several lines of evidence favor a high flux in the post-tessera period but we have no independent evidence for the absolute duration of ridged plains emplacement. During this time, the net state of stress in the lithosphere apparently changed from extensional to compressional, first in the form of extensive ridge belt development, followed by the formation of extensive wrinkle ridges on the flow units. Subsequently, there occurred local emplacement of smooth and lobate plains units which are presently essentially undefortned. The major events in the latest 10% of the presently preserved history of Venus (less than 50 m.y. ago) are continued rifting and some associated volcanism, and the redistribution of eolian material largely derived from impact crater deposits.

Basilevsky, Alexander T.↗

NASA NTRS2006

Development of Analytical Protocols For Organics and Isotopes Analysis on the 2009 MARS Science Laboratory.

The Mars Science Laboratory, under development for launch in 2009, is designed explore and quantitatively asses a local region on Mars as a potential habitat for present or past life. Its ambitious goals are to (1) assess the past or present biological potential of the target environment, (2) to characterize the geology and geochemistry at the MSL landing site, and (3) to investigate planetary processes that influence habitability. The planned capabilities of the rover payload will enable a comprehensive search for organic molecules, a determination of definitive mineralogy of sampled rocks and fines, chemical and isotopic analysis of both atmospheric and solid samples, and precision isotope measurements of several volatile elements. A range of contact and remote surface and subsurface survey tools will establish context for these measurements and will facilitate sample identification and selection. The Sample Analysis at Mars (SAM) suite of MSL addresses several of the mission's core measurement goals. It includes a gas chromatograph, a mass spectrometer, and a tunable laser spectrometer. These instruments will be designed to analyze either atmospheric samples or gases extracted from solid phase samples such as rocks and fines. We will describe the range of measurement protocols under development and study by the SAM engineering and science teams for use on the surface of Mars.

Mahaffy, P. R.↗

NASA NTRS2006

Thermal and Evolved Gas Analysis of Geologic Samples Containing Organic Materials: Implications for the 2007 Mars Phoenix Scout Mission

The Thermal and Evolved Gas Analyzer (TEGA) instrument scheduled to fly onboard the 2007 Mars Phoenix Scout Mission will perform differential scanning calorimetry (DSC) and evolved gas analysis (EGA) of soil samples and ice collected from the surface and subsurface at a northern landing site on Mars. We have been developing a sample characterization data library using a laboratory DSC integrated with a quadrupole mass spectrometer to support the interpretations of TEGA data returned during the mission. The laboratory TEGA test-bed instrument has been modified to operate under conditions similar to TEGA, i.e., reduced pressure (e.g., 100 torr) and reduced carrier gas flow rates. We have previously developed a TEGA data library for a variety of volatile-bearing mineral phases, including Fe-oxyhydroxides, phyllosilicates, carbonates, and sulfates. Here we examine the thermal and evolved gas properties of samples that contain organics. One of the primary objectives of the Phoenix Scout Mission is to search for habitable zones by assessing organic or biologically interesting materials in icy soil. Nitrogen is currently the carrier gas that will be used for TEGA. In this study, we examine two possible modes of detecting organics in geologic samples; i.e., pyrolysis using N2 as the carrier gas and combustion using O2 as the carrier gas.

Lauer, H. V., Jr.↗

NASA NTRS2011

Multiangle Implementation of Atmospheric Correction (MAIAC): 2. Aerosol Algorithm

An aerosol component of a new multiangle implementation of atmospheric correction (MAIAC) algorithm is presented. MAIAC is a generic algorithm developed for the Moderate Resolution Imaging Spectroradiometer (MODIS), which performs aerosol retrievals and atmospheric correction over both dark vegetated surfaces and bright deserts based on a time series analysis and image-based processing. The MAIAC look-up tables explicitly include surface bidirectional reflectance. The aerosol algorithm derives the spectral regression coefficient (SRC) relating surface bidirectional reflectance in the blue (0.47 micron) and shortwave infrared (2.1 micron) bands; this quantity is prescribed in the MODIS operational Dark Target algorithm based on a parameterized formula. The MAIAC aerosol products include aerosol optical thickness and a fine-mode fraction at resolution of 1 km. This high resolution, required in many applications such as air quality, brings new information about aerosol sources and, potentially, their strength. AERONET validation shows that the MAIAC and MOD04 algorithms have similar accuracy over dark and vegetated surfaces and that MAIAC generally improves accuracy over brighter surfaces due to the SRC retrieval and explicit bidirectional reflectance factor characterization, as demonstrated for several U.S. West Coast AERONET sites. Due to its generic nature and developed angular correction, MAIAC performs aerosol retrievals over bright deserts, as demonstrated for the Solar Village Aerosol Robotic Network (AERONET) site in Saudi Arabia.

Lyapustin, A.↗

NASA NTRS2018

Simulating Forest Dynamics of Lowland Rainforests in Eastern Madagascar

Ecological modeling and forecasting are essential tools for the understanding of complex vegetation dynamics. The parametric demands of some of these models are often lacking or scant for threatened ecosystems, particularly in diverse tropical ecosystems. One such ecosystem and also one of the world's biodiversity hotspots, Madagascar's lowland rainforests, have disappeared at an alarming rate. The processes that drive tree species growth and distribution remain as poorly understood as the species themselves. We investigated the application of the process-based individual-based FORMIND model to successfully simulate a Madagascar lowland rainforest using previously collected multi-year forest inventory plot data. We inspected the model's ability to characterize growth and species abundance distributions over the study site, and then validated the model with an independently collected forest-inventory dataset from another lowland rainforest in eastern Madagascar. Following a comparative analysis using inventory data from the two study sites, we found that FORMIND accurately captures the structure and biomass of the study forest, with r(squared) values of 0.976, 0.895, and 0.995 for 1:1 lines comparing observed and simulated values across all plant functional types for aboveground biomass (tonnes/ha), stem numbers, and basal area (m(squared)/ha), respectively. Further, in validation with a second study forest site, FORMIND also compared well, only slightly over-estimating shade-intermediate species as compared to the study site, and slightly under-representing shade-tolerant species in percentage of total aboveground biomass. As an important application of the FORMIND model, we measured the net ecosystem exchange (NEE, in tons of carbon per hectare per year) for 50 ha of simulated forest over a 1000-year run from bare ground. We found that NEE values ranged between 1 and -1 t Cha(exp -1)year(exp -1), consequently the study forest can be considered as a net neutral or a very slight carbon sink ecosystem, after the initial 130 years of growth. Our study found that FORMIND represents a valuable tool toward simulating forest dynamics in the immensely diverse Madagascar rainforests.

Forest Productivity↗

NASA NTRS2020

Structural evolution, electrochemical kinetic properties, and stability of A-site doped perovskite Sr(1-x)Yb(x)CoO(3-δ)

Mixed ionic and electronic conducting (MIEC) perovskite SrCoO(3-δ) is a widely studied (electro)catalyst for the oxygen reduction reaction (ORR) and possesses different crystal structures at different temperatures. These temperature dependent phase transitions significantly impact the ordering of oxygen vacancies and electrochemical kinetic properties as well as the reliability of the related devices. Some of the crystal structures formed, e.g. hexagonal phases, turn out to be almost impermeable to oxygen gas. Therefore, it is important to stabilize the crystal structure of SrCoO(3-δ) that favors the ORR over a wide temperature range. Herein, the partial substitution of the A-site Sr with Yb is systematically studied, including synthesis, characterization and analysis of structural evolution, electrochemical kinetic properties, thermal stability, and stability in a CO2-containing atmosphere. The results indicate that Sr(0.90)Yb(0.10)CoO(3-δ) is able to stabilize the tetragonal crystal structure with less ordered oxygen vacancies, leading to polarization resistances of 0.051, 0.115 and 0.272 U sq.cm at 750, 700 and 650 °C, respectively, on symmetrical cells. Sr(0.90)Yb(0.10)CoO(3-δ) demonstrates a very stable surface oxygen vacancy distribution and electronic structure near oxygen vacancies but dissociation of adsorbed oxygen molecules into atomic oxygen is affected by surface Sr segregation, and polarization resistance degradation is mainly induced by surface Sr segregation. Furthermore, Sr(0.90)Yb(0.10)CoO(3-δ) exhibits excellent thermal stability as well as excellent recovery stability and improved polarization performance after a few pure air/CO2-containing air treatment cycles at 700 °C. However, a hysteresis behavior of polarization performance is observed at 650 °C during gas cycling treatment, which may cause long-term degradation of the Sr(0.90)Yb(0.10)CoO(3-δ) electrode. The different polarization behaviors during gas cycling treatment are induced by different sensitivities of the formed surface strontium carbonate and chemisorbed surface oxo-carbonaceous species to different operating temperatures.

electrochemical kinetic↗

NASA NTRSDate not supplied

Volume Raycasting of GNSS Signals through Ground Structure Lidar for UAV Navigational Guidance and Safety Estimation

Autonomous UAS navigation at low altitudes is often hindered by degradation of GNSS position estimates. The line of sight from the UAS to orbital satellites may be intersected by foliage (which attenuates the received signal) and by buildings (which block the signal). Since the geometric ray from the presumed UAS position to each GNSS satellite orbital location is predictable, if a 3D survey of ground structures is available, the degree of blockage of each GNSS signal can be estimated. In this study we show raycasting from a UAS location to GNSS satellites at two flight locations: one with overlying structures and bordered by tall trees, and another in an arboreal canyon bordered by tall trees. We confirm the intermittent blockage of satellites in the first location sufficient to lose GNSS position fix. We demonstrate low-altitude GNSS fidelity forecasting via the raycasting method at the second location that can be used to plan navigable flight locations and altitudes. Finally, we match the GNSS signal strength with raycast-derived foliage obstruction depth at hundreds of observation times from 55 recordings collected over 14 days from November 2018 to February 2021 at the second location. This matching confirms that signal attenuation varies with the depth of foliage blockage along a saturating exponential curve, as found in prior continuous-wave RF studies. The exponent and saturation value are species dependent and therefore vary from site to site; once determined empirically, they can be used to characterize foliage along a particular flight path, and refine GNSS fidelity forecasts of flights along that path. The techniques described in this study show the feasibility of a survey method to construct low-altitude navigation safety maps and forecasts.

Navigation↗

NASA NTRS1994

Carbon monoxide measurements at Mace Head, Ireland

The North Atlantic Ocean is bordered by continents which may each, under the influence of seasonal weather patterns, act as sources of natural and anthropogenic trace gas and particulate species. Photochemically active species such as carbon monoxide (CO) react to form ozone (O3), a species of critical importance in global climate change. CO is sparingly soluble in water, and the relatively long lifetime of CO in the troposphere makes this species an ideal tracer of air masses with origin over land. We have measured CO using a nondispersive infrared gas filter correlation analyzer at Mace Head on the west coast of Ireland nearly continuously since August 9, 1991. Measurements of CO were acquired at 20-sec resolution and recorded as 60-sec averages. Daily, monthly, and diurnal variation data characteristics of CO mixing ratios observed at this site are reported. Depending on source regions of air parcels passing over this site, 60-min concentrations of CO range from clean air values of approximately 90 ppbv to values in excess of 300 ppbv. Data characterizing the correlation between 60-min CO and O3 mixing ratio data observed at this site are reported also.

Doddridge, Bruce G.↗

NASA NTRS2002

Rationally designed fluorescently labeled sulfate-binding protein mutants: evaluation in the development of a sensing system for sulfate

Periplasmic binding proteins from E. coli undergo large conformational changes upon binding their respective ligands. By attaching a fluorescent probe at rationally selected unique sites on the protein, these conformational changes in the protein can be monitored by measuring the changes in fluorescence intensity of the probe which allow the development of reagentless sensing systems for their corresponding ligands. In this work, we evaluated several sites on bacterial periplasmic sulfate-binding protein (SBP) for attachment of a fluorescent probe and rationally designed a reagentless sensing system for sulfate. Eight different mutants of SBP were prepared by employing the polymerase chain reaction (PCR) to introduce a unique cysteine residue at a specific location on the protein. The sites Gly55, Ser90, Ser129, Ala140, Leu145, Ser171, Val181, and Gly186 were chosen for mutagenesis by studying the three-dimensional X-ray crystal structure of SBP. An environment-sensitive fluorescent probe (MDCC) was then attached site-specifically to the protein through the sulfhydryl group of the unique cysteine residue introduced. Each fluorescent probe-conjugated SBP mutant was characterized in terms of its fluorescence properties and Ser171 was determined to be the best site for the attachment of the fluorescent probe that would allow for the development of a reagentless sensing system for sulfate. Three different environment-sensitive fluorescent probes (1,5-IAEDANS, MDCC, and acylodan) were studied with the SBP171 mutant protein. A calibration curve for sulfate was constructed using the labeled protein and relating the change in the fluorescence intensity with the amount of sulfate present in the sample. The detection limit for sulfate was found to be in the submicromolar range using this system. The selectivity of the sensing system was demonstrated by evaluating its response to other anions. A fast and selective sensing system with detection limits for sulfate in the submicromolar range was developed. Copyright 2002 Wiley Periodicals, Inc. Biotechnol Bioeng 78: 517-526, 2002.

Evaluation Studies↗