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At least 289 records · Page 16

A versatile high-speed x-ray microscope for sub-10 nm imaging

We have developed a next-generation scanning x-ray microscope RASMI (RApid Scanning Microscopy Instrument) for high-throughput tomographic imaging. RASMI is installed at the hard x-ray nanoprobe beamline at NSLS-II and is capable of manipulating 1D multilayer Laue lenses (MLLs) and 2D optics (both zone plates and monolithically assembled 2D MLLs). The sample scanning stage utilizes line-focusing interferometry as an encoder while performing fly-scanning data acquisition. The system can be configured for both position- and time-triggering modes during fly-scanning. The microscope demonstrated a detector-limited data acquisition rate of 1.25 kHz during ptychography measurements. The initial x-ray results yielded a sample-limited resolution of ∼6 nm in 2D. RASMI can be adopted for in-vacuum applications and is a foundation for the next-generation scanning microscopy systems to be developed and commissioned at NSLS-II.

36 MATERIALS SCIENCE↗

Geometric Assessment of PlanetScope Imagery

Commercial companies such as Maxar, Planet Labs, and BlackSky have built large archives of high resolution (1-3 m) Earth observing data. The high temporal resolution and global coverage of these satellites makes commercial satellite images ideal for scientists studying rapidly changing processes such as flooding and fires. The high spatial resolution allows scientists to push studies to a fine scale such as monitoring agricultural growth experiments or detecting vehicles for traffic management. Scientists using these data need to know geolocation accuracy, band-to-band registration (BBR), and pixel footprint size to best determine appropriate applications as well as what extra steps they will need to take to use the data. Here, we find those key geometric properties for images from Planet Labs’ SuperDove constellation. Geolocation accuracy is assessed relative to WorldView images at globally distributed cities, band-to-band registration (BBR) is examined for every image we acquired (200+ images), and pixel footprint sizes are calculated from edge responses of the images over Cal/Val sites in China, India, or USA both near launch time and 1+ years after launch. We find that SuperDove geolocation is highly self-consistent with average global offsets among SuperDove images of 3.80 m (1.27 pixels). Despite this self-consistency, relative geolocation accuracy (with WorldView images as reference) varies by location from 3.20 m – 28.09 m mean offset. SuperDove BBR is sub-pixel and varies by band, with a mean radial offset relative to red band varying from 0.39 - 1.13 m. SuperDove image footprint size improves after 1+ years in orbit, with an average footprint size of 3.41 pixels (10.24 m) soon after launch and 3.23 pixels (9.7 m) 1+ years after launch. The BBR and self-consistency offsets are relatively small, given the large footprint sizes. A similarly extensive investigation is in progress for Planet Labs’ Dove-R series.

Alana G. Semple↗

Instrument for Achieving High Angular Resolution on the Infrared Telescope

Aberrations in stellar images caused by the atmosphere sets a significant limit on angular resolution in ground based astronomy. The largest of these aberrations is the image motion or wavefront tilt. Since the image motion is random it causes a blurring of the image, and this causes a blurring of the image from 0.3 arcseconds to about 0.7 arcseconds. The purpose of the tip-tilt project was to devise a system for the NASA Infrared Telescope Facility that would measure the image movement and correct it by rapidly tilting a mirror in two axes (tip and tilt). The system would involve building a CCD sensor package to measure the image motion, a new top end for the telescope to hold the tip-tilt mirror, a control system, and software. The system was designed to correct images for the facility camera, NSFCAM, and for the facility spectrometer, SPEX. Both of these instruments are equipped with a cold beamsplitter to feed the sensor package.

Hall, Donald N. B.↗

Crack Imaging and Quantification in Aluminum Plates with Guided Wave Wavenumber Analysis Methods

Guided wavefield analysis methods for detection and quantification of crack damage in an aluminum plate are presented in this paper. New wavenumber components created by abrupt wave changes at the structural discontinuity are identified in the frequency-wavenumber spectra. It is shown that the new wavenumbers can be used to detect and characterize the crack dimensions. Two imaging based approaches, filter reconstructed imaging and spatial wavenumber imaging, are used to demonstrate how the cracks can be evaluated with wavenumber analysis. The filter reconstructed imaging is shown to be a rapid method to map the plate and any existing damage, but with less precision in estimating crack dimensions; while the spatial wavenumber imaging provides an intensity image of spatial wavenumber values with enhanced resolution of crack dimensions. These techniques are applied to simulated wavefield data, and the simulation based studies show that spatial wavenumber imaging method is able to distinguish cracks of different severities. Laboratory experimental validation is performed for a single crack case to confirm the methods' capabilities for imaging cracks in plates.

Yu, Lingyu↗

ERTS-1 imagery as an aid to the understanding of the regional setting of base metal deposits in the North West Cape Province, South Africa

A number of base metal finds have recently focussed attention on the North Western Cape Province of South Africa as an area of great potential mineral wealth. From the point of view of competitive mineral exploration it was essential that an insight into the regional geological controls of the base metal mineralization of the area be obtained as rapidly as possible. Conventional methods of producing a suitable regional geological map were considered to be too time-consuming and ERTS-1 imagery was consequently examined. This imagery has made a significant contribution in the compilation of a suitable map on which to base further mineral exploration programmes. The time involved in the compilation of maps of this nature was found to be only a fraction of the time necessary for the production of similar maps using other methods. ERTS imagery is therefore considered to be valuable in producing accurate regional maps in areas where little or no geological data are available, or in areas of poor access. Furthermore, these images have great potential for rapidly defining the regional extent of metallogenic provinces.

Viljoen, R. P.↗

A new μ -high energy resolution fluorescence detection microprobe imaging spectrometer at the Stanford Synchrotron Radiation Lightsource beamline 6-2

In this report we describe a new synchrotron X-ray Fluorescence (XRF) imaging instrument with an integrated High Energy Fluorescence Detection X-ray Absorption Spectroscopy (HERFD-XAS) spectrometer at the Stanford Synchrotron Radiation Lightsource at beamline 6-2. The X-ray beam size on the sample can be defined via a range of pinhole apertures or focusing optics. XRF imaging is performed using a continuous rapid scan system with sample stages covering a travel range of 250 × 200 mm 2 , allowing for multiple samples and/or large samples to be mounted. The HERFD spectrometer is a Johann-type with seven spherically bent 100 mm diameter crystals arranged on intersecting Rowland circles of 1 m diameter with a total solid angle of about 0.44% of 4π sr. A wide range of emission lines can be studied with the available Bragg angle range of ~64.5°–82.6°. With this instrument, elements in a sample can be rapidly mapped via XRF and then selected features targeted for HERFD-XAS analysis. Furthermore, utilizing the higher spectral resolution of HERFD for XRF imaging provides better separation of interfering emission lines, and it can be used to select a much narrower emission bandwidth, resulting in increased image contrast for imaging specific element species, i.e., sparse excitation energy XAS imaging. This combination of features and characteristics provides a highly adaptable and valuable tool in the study of a wide range of materials.

47 OTHER INSTRUMENTATION↗

Machine learning assisted quantum super-resolution microscopy

One of the main characteristics of optical imaging systems is spatial resolution, which is restricted by the diffraction limit to approximately half the wavelength of the incident light. Along with the recently developed classical super-resolution techniques, which aim at breaking the diffraction limit in classical systems, there is a class of quantum super-resolution techniques which leverage the non-classical nature of the optical signals radiated by quantum emitters, the so-called antibunching super-resolution microscopy. This approach can ensure a factor of $\sqrt{n}$ improvement in the spatial resolution by measuring the n -th order autocorrelation function. The main bottleneck of the antibunching super-resolution microscopy is the time-consuming acquisition of multi-photon event histograms. We present a machine learning-assisted approach for the realization of rapid antibunching super-resolution imaging and demonstrate 12 times speed-up compared to conventional, fitting-based autocorrelation measurements. The developed framework paves the way to the practical realization of scalable quantum super-resolution imaging devices that can be compatible with various types of quantum emitters.

71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSIC↗

Methods and systems of proteome analysis and imaging

Provided herein are methods and systems for proteome analysis that are at least partially automated and/or performed robotically. In some aspects, the methods and systems described herein can rapidly and efficiently provide protein identification of each of the proteins from a proteome, or a complement of proteins, obtained from extremely small amounts of biological samples. The identified proteins can be imaged quantitatively over a spatial region. Automation and robotics facilitates the throughput of the methods and systems, which enables protein imaging and/or rapid proteome analysis.

Piehowski, Paul D.↗

High Accuracy In-Flight Wavelength Calibration of Imaging Spectrometry Data

Accurate wavelength calibration of imaging spectrometer data is essential if proper atmospheric transmission corrections are to be made to obtain apparent surface reflectance. Accuracies of 0.1 nm are necessary for a 10 nm-sampling instrument in order to match the slopes of the deep atmospheric water vapor features that dominate the 0.7-2.3 micrometer regions. The Airborne Visible/Infrared Imaging Spectrometer (AVIRIS) is calibrated in the laboratory to determine the wavelength position and full-width-half-maximum (FWHM) response for each of the 224 channels. The accuracies are limited by the quality of the monochromator used as a source. The accuracies vary from plus or minus to plus or minus 1.5 nm depending on the wavelength region, in general decreasing with increasing wavelength. Green et al. make corrections to the wavelength calibrations by using the known positions of 14 atmospheric absorption features throughout the 0.4-2.5 micrometer wavelength region. These features, having varying width and intensity, were matched to the MODTRAN model with a non-linear least squares fitting algorithm. A complete calibration was developed for all 224 channels by interpolation. Instrument calibration cannot be assumed to be stable to 0.1 nm over a flight season given the rigors of thermal cycling and launch and landing loads. The upcoming sensor HYDICE will require a means for in-flight spectral calibration of each scene because the calibration is both temperature and pressure sensitive. In addition, any sensor using a two-dimensional array has the potential for systematic wavelength shifts as a function of cross-track position, commonly called 'smile'. Therefore, a rapid means for calibrating complete images will be required. The following describes a method for determining instrument wavelength calibration using atmospheric absorption features that is efficient enough to be used for entire images on workstations. This study shows the effect of the surface reflectance on the calibration accuracy and the calibration history for the AVIRIS B spectrometer over the 1992 flight season.

Goetz, Alexander F. H.↗

Solar flare gamma-ray and hard x ray imaging with the GRID-on-a-balloon

A primary scientific objective for solar flare research during the rapidly approaching maximum in solar activity is the imaging of gamma-ray and hard x ray sources of solar flare emissions. These goals will be pursued by the Gamma Ray Imaging Device (GRID) instrument, one of three instruments recently selected for NASA's Max '91 Solar Balloon Program. The GRID instrument is based on the technique of Fourier transform imaging and utilizes scanning modulation grid collimator optics to provide full-Sun imaging with 1.9-arcsecond resolution over the energy range from 20 to 700 keV at time resolutions from 0.1 to 2 s. The GRID telescope will employ 32 subcollimators, each composed of a matched pair of high-Z collimator grids separated by 5.2 meters and a phoswich scintillation spectrometer detector having no spatial resolution. The subcollimators and integrally-mounted fine aspect system are contained within a telescope canister which will be pointed to 0.1 degree accuracy and cyclically scanned to produce source modulation. The 32 subcollimators provide a uniform distribution of grid slit orientations and a logarithmic distribution of slit spacings corresponding to angular dimensions of 1.9 arcseconds to several arcminutes. The instrument is several orders of magnitude more sensitive than the HXIS instrument on the Solar Maximum Mission (SMM) and nearly 10 times more sensitive than any similar instrument scheduled to fly during the next solar maximum. The payload, designed for long-duration high-altitude balloon capability, is scheduled for its first science flight (8 to 14 days duration) from the Antarctic in January of 1992.

Orwig, Larry E.↗

Segmentation method comparison for residual fiber length measurement across tiled microscopy images

Fiber length distribution (FLD), in part, governs mechanical properties in discontinuous fiber composites, yet manual measurement methods limit the high-throughput characterization needed for materials design optimization. This study compares deep learning segmentation approaches for automated FLD measurement in large-field microscopy, evaluating how method choice affects the microstructural descriptors used in structure-property-processing relationships. A critical challenge is that high-resolution microscopy images (10,000×10,000 pixels) must be tiled for deep learning analysis, fragmenting fibers at boundaries. We demonstrate that segmentation method proves crucial for measurement accuracy. For example, instance segmentation with Slicing Aided Hyper Inference (SAHI) preserves individual fiber integrity across tiles while semantic segmentation prioritizes speed. Comparing against manual measurement of extracted carbon fibers, YOLOv11-SAHI matched manual ground truth (238 μm weighted mean) with 40x speedup (4.5 vs 167 minutes per image). U-Net provides rapid quantification although it is at the cost of reduced accuracy due only reliably measuring stand-alone fibers. Our comparative analysis reveals that instance segmentation with SAHI better preserves length measurements while semantic segmentation prioritizes speed, providing empirical guidance for method selection. The characterization provides essential inputs for mechanical property prediction models and inverse design workflows, accelerating composite materials development cycles.

Additive manufacturing↗

Projection system for display of parallax and perspective

A projection system for the display of parallax and perspective of a real image from a hologram is presented. A reference beam was projected in a sequence of several projections at selected angles of perspective through the hologram, this sequence being rapidly performed. The resulting angular spaced images emitting from the hologram were directed onto a mirror which was coordinately tilted to reflect all of the resulting images to register onto a screen where they appeared as a single three dimensional image.

Kurtz, R. L.↗

BigView Image Viewing on Tiled Displays

BigView allows for interactive panning and zooming of images of arbitrary size on desktop PCs running Linux. Additionally, it can work in a multi-screen environment where multiple PCs cooperate to view a single, large image. Using this software, one can explore on relatively modest machines images such as the Mars Orbiter Camera mosaic [92,160 33,280 pixels]. The images must be first converted into paged format, where the image is stored in 256 256 pages to allow rapid movement of pixels into texture memory. The format contains an image pyramid : a set of scaled versions of the original image. Each scaled image is 1/2 the size of the previous, starting with the original down to the smallest, which fits into a single 256 x 256 page.

Sandstrom, Timothy↗

Assessing the Needs of NASA's Near Real-Time Earth Observation Products

"The 2017-2027 Decadal Survey for Earth Science and Applications from Space stated that NASA's Earth Science with planned implementation of applications provides sustained earth observations for societal benefits [1]. The Decadal Survey indicated that data latency is invaluable for time-sensitive applications including disaster risk reduction, wildland fire carbon emissions quantification, real-time measurements of the state of the hydrologic systems and many more. Data latency refers to the time between earth observation and data products available to users. During the past 13 years, NASA's Land, Atmosphere Near Real-Time Capability for Earth Observing Systems (LANCE) continues to provide free access to earth observation products that are made available much quicker than routine processing allows. The latency of most LANCE data products is Near Real-time (NRT) which is defined as less than three hours from satellite observations [2]. LANCE is managed by the Earth Science Data and Information System (ESDIS) Project at NASA Goddard Space Flight Center [3], and a User Working Group (UWG) is responsible for providing guidance to LANCE. LANCE data are used by direct users and brokers who add value to the data [4]. NASA Earth Applied Sciences Program (ASP) is one of the primary users of LANCE, which collaborates with partner organizations and provides support to scientists to solve problems in applications of earth observations. ASP promotes the use of LANCE NRT data products to demonstrate applications in decision making, facilitates end-user feedback to the science team to improve data products, and provides information on future demands for research. LANCE supports applications that need a rapid response including detecting wildland fires and volcanic eruptions, tracking smoke, ash and dust plumes, monitoring air quality and tracking extreme weather events such as hurricanes, landslides, and floods. To gather feedback regarding the availability, accessibility and actionability of NASA's NRT data products for societal benefit, three surveys and a few discussions with experts involved in the topic within ASP were conducted from the perspective of users. Feedback has been collected from users who are interested in using low latency NASA data within application communities of agriculture, disasters, water resources, health and air quality, ecological conservation, wildland fires and capacity building. Analysis-ready NRT data products in a variety of formats have been mentioned many times in the collected feedback, especially for applied users with little to no experience using research-grade earth observation products. Users prefer to have products that can be easily integrated into their existing workflows and take their analysis to the data. HDF5 is a commonly used data format for research, but typically requires some conversion to a more friendly format for applications and regular use in decision-making. Users prefer the GeoTIFF data format that can be directly ingested into a GIS mapping software and platform for data analysis and visualization. For example, LANCE’s fire, flood, SO2 and Black Marble Nighttime Blue/Yellow Composite data products have been integrated into NASA Disasters Mapping Portal, which is an GIS-based open data portal, for users in the disaster management community. There are 291 LANCE NRT layers available through GIBS and Worldview, where users can download a snapshot in GeoTIFF format. Operational users expect data to be processed as close to the user as possible. The collected feedback indicates that LANCE fire products within 3 hours latency would meet the needs of the wildland fire community. The ideal latency for volcanic application is 10-15 minutes. Users in Volcanic Ash Advisory Centers (VAAC) reported that the first forecast volcanic product should be issued within 75 minutes from the volcano eruption [5]. Overall, for disaster applications, data latency within 3 hours is useful while latency greater than 12 hours is not timely enough for operational use. Capacity building and training are critical for users to be able to access, interpret and use data products and tools for their decision making, especially for applied users with limited experience using earth observation products. LANCE data products have been used in a number of capacity building projects domestically and internationally [6]. As LANCE continues to bring new products into the system, users request training to utilize LANCE new and upcoming data products and capabilities in their applications. Due to the limitation of bandwidth and downstream flow paths, users in some developing countries need tools to select and download data for a specific area of interest instead of bulk downloads. The collected feedback also shows the lack of available SAR satellite low latency data products. The advantages of SAR to monitor conditions and changes on the ground through darkness, clouds, volcanic ash, and other atmospheric conditions, are appealing to low latency users. For example, terabytes of low latency but cloudy optical images are not helpful in rapidly identifying the extent of flood or fire impacts. LANCE could be complemented with low latency measurements via the upcoming NASA-ISRO Synthetic Aperture Radar (NISAR) mission [7]. Requests for higher spatial resolution products are expressed. A user from the wildland fire management community reported that products with 30-m spatial resolution could be used to detect small fires. The 30-m Landsat OLI fire data is now part of NASA’s Fire Information for Resource Management System (FIRMS) US/Canada [8]. Within the open and free NASA resources, LANCE disseminates NRT data products in a manner that allows them to be accessible and understandable to both scientific and applied users. In many application areas, latency plays an important or even decisive role where low latency earth observations help people to observe areas of interest, detect and track changes in the environment and make timely decisions. NASA’s Earth Applied Sciences Program promotes the use of LANCE NRT products and builds a bridge between application users and research teams. The collected feedback indicates data latency within 3 hours is useful for most of the applications, and shows the needs of user-friendly, analysis-ready products, and requests training on LANCE’s new and upcoming data products. User feedback has been provided to LANCE UWG for guidance and recommendations, and for translating findings into something actionable.

Tian Yao↗

Ultra-High Temperature Materials Characterization for Propulsion Applications

Propulsion system efficiency increases as operating temperatures are increased. Some very high-temperature materials are being developed, including refractory metal alloys, carbides, borides, and silicides. System design requires data for materials properties at operating temperatures. Materials property data are not available for many materials of interest at the desired operating temperatures (up to approx. 3000 K). The objective of this work is to provide important physical property data at ultra-high temperatures. The MSFC Electrostatic levitation (ESL) facility can provide measurements of thermophysical properties which include: creep strength, density and thermal expansion for materials being developed for propulsion applications. The ESL facility uses electrostatic fields to position samples between electrodes during processing and characterization studies. Because the samples float between the electrodes during studies, they are free from any contact with a container or test apparatus. This provides a high purity environment for the study of high-temperature, reactive materials. ESL can be used to process a wide variety of materials including metals, alloys, ceramics, glasses and semiconductors. The MSFC ESL has provided non-contact measurements of properties of materials up to 3400 C. Density and thermal expansion are measured by analyzing digital images of the sample at different temperatures. Our novel, non-contact method for measuring creep uses rapid rotation to deform the sample. Digital images of the deformed samples are analyzed to obtain the creep properties, which match those obtained using ASTM Standard E-139 for Nb at 1985 C. Data from selected ESL-based characterization studies will be presented. The ESL technique could support numerous propulsion technologies by advancing the knowledge base and the technology readiness level for ultra-high temperature materials. Applications include non-eroding nozzle materials and lightweight, high-temperature alloys for turbines and structures.

Rogers, Jan↗

Listeria monocytogenes loss of cultivability on carrot is associated with the formation of mesosome-like structures

Raw carrot is known to have antimicrobial activity against Listeria monocytogenes, but the mechanism of action has not been fully elucidated. In this study, we examined carrot antilisterial activity against several strains of Listeria species (including L. grayi, L. innocua, L. seeligeri, and L. welshimeri) and L. monocytogenes. A representative strain of L. monocytogenes was subsequently used for further characterizing carrot antilisterial activity. Exposure to fresh-cut carrot for 15 min resulted in a similar loss of cultivability, ranging from 2.5 to 4.7 log units, across all Listeria strains evaluated. L. monocytogenes recovered from the fresh-cut surface of different raw carrots was 1.6 to 4.1 log lower than levels obtained from paired boiled carrot samples with abolished antilisterial activity. L. monocytogenes levels recovered from fresh-cut carrot were 2.8 to 3.1 log lower when enumerated by culture-dependent methods than by the culture-independent method of PMAxx-qPCR, a qPCR assay that is performed using DNA pre-treated to selectively sequester DNA from cells with injured membranes. These results suggested that L. monocytogenes loss of cultivability on fresh-cut carrot was not associated with a loss of L. monocytogenes cell membrane integrity and putative cell viability. Transmission electron microscopy imaging revealed that L. monocytogenes rapidly formed mesosome-like structures upon exposure to carrot fresh-cut surface but not upon exposure to boiled carrot surface, suggesting there may be an association between the formation of these mesosome-like structures and a loss of cultivability in L. monocytogenes. As a result, further research is necessary to conclude the causality of this association.

59 BASIC BIOLOGICAL SCIENCES↗

Advances in the photon avalanche luminescence of inorganic lanthanide-doped nanomaterials

Photon avalanche (PA)—where the absorption of a single photon initiates a ‘chain reaction’ of additional absorption and energy transfer events within a material—is a highly nonlinear optical process that results in upconverted light emission with an exceptionally steep dependence on the illumination intensity. Over 40 years following the first demonstration of photon avalanche emission in lanthanide-doped bulk crystals, PA emission has been achieved in nanometer-scale colloidal particles. The scaling of PA to nanomaterials has resulted in significant and rapid advances, such as luminescence imaging beyond the diffraction limit of light, optical thermometry and force sensing with (sub)micron spatial resolution, and all-optical data storage and processing. In this review, we discuss the fundamental principles underpinning PA and survey the studies leading to the development of nanoscale PA. Finally, we offer a perspective on how this knowledge can be used for the development of next-generation PA nanomaterials optimized for a broad range of applications, including mid-IR imaging, luminescence thermometry, (bio)sensing, optical data processing and nanophotonics.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗