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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.
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Fluorometry, gas chromatography and optical resolution, mass spectrometry, computer managed instrumentation, and ultraviolet microspectrometry in exobiology studies
Survival and growth of Bacillus cereus and B. subtilis in simulated Martian environment of diurnal temperature cycling and low moisture and oxygen level
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The deployment of nuclear energy globally is hindered by the fear cleared by nuclear reactor disasters such as the Chernobyl and Fukushima reactor meltdowns. Additionally, accumulation of radioactive waste globally will, in the near future, present a significant threat to humans and the environment. Notably, the majority of nuclear waste from the energy generation industry is generated as Low Level Waste (LLW) in which the small radioactive component renders the bulk of the waste unsuitable for conventional treatment and disposal. In the case of radioactive graphite, the small radioactive component comprising radiocarbon-14 (C-14) and metallic pollutants can be removed to allow safe disposal or reuse as of the graphite. In the current study, mixed-cultures of organisms isolated from oil contaminated sites showed potential of selective bioaccumulation of C-14 from a powdered C-12/C-14 graphite matrix. The study was conducted in a continuously mixed reactor (growth vessel) constructed from Pyrex panels (30 cm x 20 cm x 15 cm) with finely ground graphite as the principal carbon source (1500-2000 mg.L{sup -1}) and spikes of glucose (500 mg.L{sup -1} every 48 hours). The growth vessel was connected to a biofilter unit by submersible pump where particulate matter was isolated for further analysis. Results showed that approximately 68% more C-14 was released from ignited dry cells than from respiration product. C-14 accumulated in cells corrected using a trap filter system, with a marked decrease of C-14 in carbon dioxide than vegetative suspended cells in the main tank. The only mechanism that could account for the differential C-14 distribution between the liquid medium and cells was possible fractionation during uptake of carbon compounds. The discriminating factor suggested in this study is the kinetic isotope effect (KIE) which allows heavier atoms to have different affinities for reaction sites on catalysis and biocatalysis. (authors)
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We described a mass spectrometry-based assay to rapidly quantify the production of primary alcohols directly from cell cultures. This novel assay used the combination of TEMPO-based oxidation chemistry and oxime ligation, followed by product analysis based on Nanostructure-Initiator Mass Spectrometry. This assay enables quantitative monitor both C5 to C18 alcohols as well as glucose and gluconate in the growth medium to support strain characterization and optimization. We find that this assay yields similar results to gas chromatography for isoprenol production but required much less acquisition time per sample. We applied this assay to gain new insights into P. Putida's utilization of alcohols and find that this strain largely could not grow on heptanol and octanol.
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