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2023 Cleanroom Monitoring for OSIRIS-REx

OSIRIS-REx is an asteroid sample return mission that delivered asteroid regolith from Bennu to Earth on 9/24/2023 [1]. Cleanroom monitoring of OSIRIS-REx Curation facilities has been ongoing since 2021 when construction of the OSIRIS-REx lab at Johnson Space Center (JSC) was completed [2]. The monitoring continues inside of the OSIRIS-REx lab in Houston. Monitoring was also conducted on a temporary cleanroom constructed inside a hangar at the Utah Test and Training Range (UTTR) to initially receive the Sample Return Capsule (SRC) upon its landing there. The following reports on cleanroom monitoring that has taken place in the months leading up to as well as after OSIRIS-REx return. Monitoring techniques used include particle counts, deployment of Si wafer witness plates (Balazs, Inc.) and Al foil witness plates, gas sampling (Balazs, Inc.) and microbiological monitoring. We collected particle counts at both the lab at JSC as well as the temporary cleanroom at UTTR. Particle counts were taken monthly from six locations in the lab at JSC. Particle counts were taken from seven locations at UTTR in July, August, and September. The UTTR cleanroom particle counts were taken daily in the five days leading to OSIRIS-REx return as well as consistently the day of return (every 2 minutes and 15 seconds 7:21 am- 6:27 pm) from one location inside the cleanroom. The particle counts at UTTR consistently stayed well within the ranges of its required ISO 7 equivalent classification (352,000 maximum particles/ m3 ≥ 0.5 μm). Most analysis showed that the particle counts were far below ISO 7 requirements (highest measured count was 61,484 particles/ m3 ≥ 0.5 um). but the elevated instances correlated to exterior hangar doors being open. Particle counts were reduced when keeping exterior hangar doors closed. The particle counts at JSC have stayed consistently within their ISO 5 equivalent classification (3,520 maximum particles/ m3 ≥ 0.5 um). In most instances, the counts are much better except in one instance where the count measured 13,381 particles/ m3 ≥ 0.5 um. The high spike in particle counts in the JSC lab correlated with the air handlers being shut down temporarily the previous day. The particle counts taken in the same location quickly reduced and measured 0 when taken a month later with the air handlers on. We deployed 8-inch semiconductor polished Si wafer witness plates for 24-hour periods inside of the OSIRIS-REx Curation lab at JSC (in July, August, September, and November 2023) and the cleanroom at UTTR (July and September 2023) to test for possible organic and inorganic contaminants. We also deployed these wafers inside of the OSIRIS-REx Touch-and-Go sample Acquisition Mechanism (TAGSAM) glovebox (where Bennu sample processing occurs) and desiccator (Bennu sample storage) at JSC in August 2023. These samples were all sent to Balazs for analysis via Thermal Desorption Gas Chromatography Mass Spectroscopy (TD-GC-MS) to quantify organic compounds and vapor phase decomposition inductively coupled plasma mass spectrometry (VPD-ICP-MS) to quantify inorganic contaminants. We collected air samples using an adsorbent tube connected to a pump for six hours (100 mL/minute) inside of both the JSC lab and the UTTR cleanroom. These samples were taken at JSC in July, August, September, November, and December and at UTTR in July and September. Additionally, a sample was taken just outside of the TAGSAM glovebox airlock door in July. These samples were sent to Balazs for analysis of volatile organics in air. Overall, the OSIRIS-REx lab at JSC and cleanroom at UTTR yielded very low organic and inorganic contaminants (similar results to Genesis lab at JSC which is ISO 4 equivalent) except for boron, which is attributed to the borosilicate glass in the fan filter units. We collected monthly surface and air samples inside the JSC lab to monitor potential microbial contamination. We report the recovery rate for each sampling event, which is defined as the number of samples exhibiting bacterial or fungal growth divided by the total number of samples collected. Since the lab was commissioned in 2021 the median recovery rate is 29%. This recovery rate is consistent with other ISO 5 equivalent labs used to curate astromaterials collections. Increases in recovery rate correspond to construction and/or extra activity in the lab. However, increases in recovery rate do not reliably correlate to increases in particle counts, which highlights the need for a dedicated microbial monitoring program for biologically sensitive collections. We sampled the temporary clean room at UTTR in July, August, and September of 2023 to monitor potential microbial contamination. The recovery rate decreased from 80% in August to 29% in September. However, in all instances, the diversity of bacteria and fungi was higher in the UTTR cleanroom than in the JSC lab. We routinely collected more than twenty different organisms from the UTTR cleanroom. In the JSC lab the median diversity is 2 organisms. Based on these results we hypothesize that microbes from UTTR could be transported back to JSC with the Bennu samples. To mitigate this risk, in September of 2023, we implemented additional cleaning procedures in the JSC lab to reduce the bioburden on surfaces that could come into direct contact with hardware used to process Bennu samples. Since adopting these additional cleaning measures, the median recovery rate has decreased to 14%. We did not observe an increase in fungal or bacterial diversity in the lab in the October sampling. We will continue to monitor this trend closely for the next several months. Supported by NASA under Award NNH09ZDA007O and Contract NNM10AA11C.

Rachel Comstock Funk↗

Mapping Rice Production in China with AVHRR Imagery

The study of rice agriculture is necessary for both the importance of rice as a vital food source and because of the fact that cultivating it has an unfortunate byproduct, namely methane gas. As a food source, rice is a staple for a large majority of the world's population, especially in Asia. Because the populations of many Asian nations are increasing at rapid rates, the production of rice will need to similarly increase. In 1989, it was estimated that the demand for rice would increase by 65% by the year 2019. Rice crops are considered to be one of the primary anthropogenic sources of methane gas. A reason for concern is that this gas is a so-called "greenhouse" trace gas and given its increasing levels in the atmosphere, is thought to contribute to the suspected global warming phenomenon. Some estimate that methane may contribute up to 20% to the global warming effect. Trace gas emissions from anthropogenic sources is an issue that generates great worldwide interest because of the fact that mankind is very likely affecting the current and future climate in potentially negative ways. In an effort to better understand these effects, scientists and engineers are conducting research on all of the varied fronts which relate to climate change and biosphere/atmosphere interactions. The study of global warming through increasing concentrations of greenhouse gases is one area which has received much media and scientific attention. Research fueled by debates on this topic is being conducted on numerous, interrelated fronts in an effort to better understand the complex relationship between human activities and the earth's climate. The research ranges from attempting to verify if the observed data even supports the existence of an anthropogenically generated global-warming phenomenon, to identification of sources and sinks of the trace gases, to measuring the source strengths, to studies which focus on modeling the processes which generate the gases, and finally, to trying to project their impact on the global climatic system. Some of the more commonly known sources of greenhouse gases are related to industry and transportation. Carbon dioxide, CO2, from automobile emissions is one such example. Lesser known are sources from natural and cultivated vegetation, such as the methane, CH4, resulting from rice cropping. While the concentration of atmospheric methane is significantly less than that of carbon dioxide, CH4 has been estimated to have up to 32 times the insulating capability of carbon dioxide, making it an important gas to monitor. The remainder of this chapter will provide some additional background information on the effects of atmospheric methane, and the role that rice agriculture plays as a source in the methane budget. This will be followed by a brief description of efforts to model this source of atmospheric methane. Finally, this chapter will end with a statement of the hypotheses of this thesis, at which time a description of the information contained in the rest of this document will be provided.

Paliouras, Eleni J.↗

Reactor/Brayton power systems for nuclear electric spacecraft

Studies are currently underway to assess the technological feasibility of a nuclear-reactor-powered spacecraft propelled by electric thrusters. This vehicle would be capable of performing detailed exploration of the outer planets of the solar system during the remainder of this century. The purpose of this study was to provide comparative information on a closed cycle gas turbine power conversion system. The results have shown that the performance is very competitive and that a 400 kWe space power system is dimensionally compatible with a single Space Shuttle launch. Performance parameters of system mass and radiator area were determined for systems from 100 to 1000 kWe. A 400 kWe reference system received primary attention. The components of this system were defined and a conceptual layout was developed with encouraging results. The preliminary mass determination for the complete power system was very close to the desired goal of 20 kg/kWe. Use of more advanced technology (higher turbine inlet temperature) will substantially improve system performance characteristics.

Layton, J. P.↗

Aluminum oxide barriers in MCrAlY superalloy systems

An investigation was made of sputtered aluminum oxide diffusion barriers to protect gas turbine engine blade and vane alloys from their coatings. MAR M200 + Hf coated with sputtered NiCoCrAlY and MAR M509 coated with sputtered FeCrAlY were obtained both with and without 1 and 2 micron sputtered Al2O3 barrier layers. Electron dispersive X-ray analysis was used to determine the concentration profiles of as-received and heat treated samples.

Kreider, Kenneth G.↗

Technology Development for Human Exploration Beyond LEO in the New Millennium IAA-13-3 Strategies and Plans for Human Mars Missions

As we look forward into the new millennium, the extension of human presence beyond Low-Earth Orbit (LEO) looms large in the plans of NASA. The Agency's Strategic Plan specifically calls out the need to identify and develop technologies for 100 and 1000-day class missions beyond LEO. To meet the challenge of these extended duration missions, it is important that we learn how to utilize the indigenous resources available to us on extraterrestrial bodies. This concept, known as In-Situ Resource Utilization (ISRU) can greatly reduce the launch mass & cost of human missions while reducing the risk. These technologies may also pave the way for the commercial development of space. While no specific target beyond LEO is identified in NASA's Strategic Plan, mission architecture studies have been on-going for the Moon, Mars, Near-Earth Asteroids and Earth/Moon & Earth/Sun Libration Points. As a result of these studies, the NASA Office of Space Flight (Code M) through the Johnson and Kennedy Space Centers, is leading the effort to develop ISRU technologies and systems to meet the current and future needs of human missions beyond LEO and on to Mars. This effort also receives support from the NASA Office of Biological and Physical Research (Code U), the Office of Space Science (Code S), and the Office of Aerospace Technology (Code R). This paper will present unique developments in the area of fuel and oxidizer production, breathing air production, water production, C02 collection, separation of atmospheric gases, and gas liquefaction and storage. A technology overview will be provided for each topic along with the results achieved to date, future development plans, and the mission architectures that these technologies support.

Larson, William E.↗

Space Launch Vehicle Transient Particle Redistribution Modeling and Implications for Optically Sensitive Payloads

The performance of contamination sensitive components—such as optical components—can be degraded by particulate matter depositing on the surfaces. Particles can accumulate during manufacturing, handling and operation. For a space-based system, particles can shed from the fairing and redistribute onto sensitive surfaces during launch. An engineering modeling approach has been developed for modeling particle migration during launch. The approach involves particle detachment from the fairing, particle transport through the venting atmosphere inside the fairing, and attachment to the receiving surface. Particle size and amounts on the fairing surface can be modeled using distributions from standards, such as IEST-STD-CC1246E, as well as from empirical data obtained from tape lifts. Surface interactions are modeled using theoretical as well as empirical data. Commercial computational fluid dynamics codes are used to calculate the gas flow in the fairing during depressurization during launch. This approach not only provides insight into particle redistribution during launch but also can be used to establish fairing cleanliness requirements.

Brieda, Lubos↗

Long-term CF6 engine performance deterioration: Evaluation of engine S/N 451-479

The performance testing and analytical teardown of CF6-6D engine is summarized. This engine had completed its initial installation on DC-10 aircraft. The investigative test program was conducted inbound prior to normal overhaul/refurbishment. The performance testing included an inbound test, a test following cleaning of the low pressure turbine airfoils, and a final test after leading edge rework and cleaning the stage one fan blades. The analytical teardown consisted of detailed disassembly inspection measurements and airfoil surface finish checks of the as received deteriorated hardware. Included in this report is a detailed analysis of the test cell performance data, a complete analytical teardown report with a detailed description of all observed hardware distress, and an analytical assessment of the performance loss (deterioration) relating measured hardware conditions to losses in both SFC (specific fuel consumption) and EGT (exhaust gas temperature).

Kramer, W. H.↗

Spontaneous Ignition Characteristics of Hydrocarbon Fuel-air Mixtures

Although the subject of spontaneous ignition of liquid fuels has received considerable attention in the past, the role of fuel evaporation in the overall spontaneous ignition process is still unclear. A main purpose of this research is to carry out measurements of ignition delay times, using fuels of current and anticipated future aeronautical interest, at test conditions that are representative of those encountered in modern gas turbine engines. Attention is focused on the fuel injection process, in particlar the measurement and control of man fuel drop size and fuel-air spatial distribution. The experiments are designed to provide accurate information on the role of fuel evaporation processes in determining the overall ignition delay time. The second objective is to examine in detail the theoretical aspects of spontaneous ignition in order to improve upon current knowledge and understanding of the basic processes involved, so that the results of the investigation can find general and widespead application.

Lefebvre, A. H.↗

A quarantine protocol for analysis of returned extraterrestrial samples

A protocol is presented for the analysis at an earth-orbiting quarantine facility of return samples of extraterrestrial material that might contain (nonterrestrial) life forms. The protocol consists of a series of tests designed to determine whether the sample, conceptualized as a 1-kg sample of Martian soil, is free from nonterrestrial biologically active agents and so may safely be sent to a terrestrial containment facility, or it exhibits biological activity requiring further (second-order) testing outside the biosphere. The first-order testing procedure seeks to detect the presence of any replicating organisms or toxic substances through a series of experiments including gas sampling, analysis of radioactivity, stereomicroscopic inspection, chemical analysis, microscopic examination, the search for metabolic products under growth conditions, microbiologicl assays, and the challenge of cultured cells with any agents found or with the extraterrestrial material as is. Detailed plans for the second-order testing would be developed in response to the actual data received from primary testing.

Bagby, J. R.↗

Feasibility of Actively Cooled Silicon Nitride Airfoil for Turbine Applications Demonstrated

Nickel-base superalloys currently limit gas turbine engine performance. Active cooling has extended the temperature range of service of nickel-base superalloys in current gas turbine engines, but the margin for further improvement appears modest. Therefore, significant advancements in materials technology are needed to raise turbine inlet temperatures above 2400 F to increase engine specific thrust and operating efficiency. Because of their low density and high-temperature strength and thermal conductivity, in situ toughened silicon nitride ceramics have received a great deal of attention for cooled structures. However, the high processing costs and low impact resistance of silicon nitride ceramics have proven to be major obstacles for widespread applications. Advanced rapid prototyping technology in combination with conventional gel casting and sintering can reduce high processing costs and may offer an affordable manufacturing approach. Researchers at the NASA Glenn Research Center, in cooperation with a local university and an aerospace company, are developing actively cooled and functionally graded ceramic structures. The objective of this program is to develop cost-effective manufacturing technology and experimental and analytical capabilities for environmentally stable, aerodynamically efficient, foreign-object-damage-resistant, in situ toughened silicon nitride turbine nozzle vanes, and to test these vanes under simulated engine conditions. Starting with computer aided design (CAD) files of an airfoil and a flat plate with internal cooling passages, the permanent and removable mold components for gel casting ceramic slips were made by stereolithography and Sanders machines, respectively. The gel-cast part was dried and sintered to final shape. Several in situ toughened silicon nitride generic airfoils with internal cooling passages have been fabricated. The uncoated and thermal barrier coated airfoils and flat plates were burner rig tested for 30 min without and with air cooling. Without cooling, the surface temperature of the flat plate reached approximately 2350 F. Starting with computer aided design (CAD) files of an airfoil and a flat plate with internal cooling passages, the permanent and removable mold components for gel casting ceramic slips were made by stereolithography and Sanders machines, respectively. The gel-cast part was dried and sintered to final shape. Several in situ toughened silicon nitride generic airfoils with internal cooling passages have been fabricated. The uncoated and thermal barrier coated airfoils and flat plates were burner rig tested for 30 min without and with air cooling. Without cooling, the surface temperature of the flat plate reached approximately 2350 F. With cooling, the surface temperature decreased to approximately 1910 F--a drop of approximately 440 F. This preliminary study demonstrates that a near-net-shape silicon nitride airfoil can be fabricated and that silicon nitride can sustain severe thermal shock and the thermal gradients induced by cooling and, thus, is a viable candidate for cooled components.

Bhatt, Ramakrishna T.↗

Synthesis of Submillimeter Radiation for Spectroscopy

The frequency-multiplier submillimeter spectrometer (FMSS) is a laboratory apparatus for far-infrared molecular spectroscopy, embodying several advances over prior such apparatuses. The most innovative part of the FMSS is a source of monochromatic submillimeter- wavelength radiation that can be tuned over a wide frequency range, as needed for trace gas analyses and molecular- structure studies for which such apparatuses are typically used. The radiation source features a modular design and is built mostly from commercially available components. It includes a computer-controlled radio-frequency synthesizer, amplifiers, and frequency multipliers of a type heretofore used in local oscillators for heterodyne far-infrared receivers. In conjunction with the rest of the apparatus, this source makes it possible to perform measurements over large portions of the submillimeter-wavelength spectrum with resolution, accuracy, and sensitivity greater than those achievable in Fourier-transform infrared spectroscopy. In comparison with prior laboratory submillimeter- wavelength radiation sources, this source is much lighter in weight, less cumbersome, less power-hungry, and capable of sustained operation with less intervention by laboratory personnel. Also, unlike some prior submillimeter-wavelength sources, this source does not require a high-voltage power source. As shown in more detail in the figure, the radiation source includes a sweep frequency synthesizer connected to an external waveform generator, the output of which is used as a reference signal. The synthesizer is computer-controlled through a standard general-purpose interface bus (GPIB) and is operated in phase-locked continuous- wave mode for all measurements. The synthesizer output ranges in frequency from 11 to 18 GHz. For suppression of frequency spurs and harmonics, the output of the frequency synthesizer is fed through a tunable yttrium iron garnet (YIG) filter, which is swept in frequency simultaneously with the frequency synthesizer.

Maiwald, Frank↗

Carbon Limited Soils of Iceland: Analogs for Identifying Carbon-Mineral Interactions in Gale Crater, Mars Surface Materials

The Mars Science Laboratory’s Curiosity rover mission to Gale Crater, Mars has a goal to search for organic carbon and corresponding evidence of ancient microbiology preserved in clay mineral-rich sedimentary deposits [1]. No clear correlation of clay mineral (smectite) abundances with the Sample Analysis at Mars (SAM) carbon detections (up to 2000 ppm C) [2] has been established, suggesting other phases may preserve organic C in martian sediments. Terrestrial volcanic glass soils can accumulate C due to the C-complexing abilities of poorly crystalline Al- and Fe-oxyhydroxides [3]. The Xray amorphous component (20 to 73 wt. %) in Gale Crater materials [4,5] has received little consideration regarding organic C preservation in Gale Crater. The objectives of this work are to 1) Evaluate C content and level of soil development of basaltic glass-bearing Icelandic soils to determine the role that poorly crystalline phases have in complexing organic C in carbon limited soils and 2) Determine if evolved gas analysis (EGA) of well-characterized Mars analog basaltic materials from Iceland can be used to interpret SAM data to understand the nature of organic-C mineral/geochemical associations in Gale materials.

B. Sutter↗

An investigation of environmental influence on the creep behavior of a low pressure plasma sprayed NiCoCrAlY alloy

Low pressure sprayed MCrAlY overlay coatings are currently being used on advanced single crystal superalloy blades for gas turbine engines. Many studies were made on the influence of coatings on the mechanical properties of superalloys in oxidizing or hot-corroding environments, but very few on the properties of the bulk coating alloy itself. The creep behavior of a typical NiCoCrAlY alloyd (PWA 276) was studied in air and vacuum. The as-received low pressure plasma sprayed NiCoCrAlY plates were heat treated for 4 h at 1080 C followed by 32 h at 870 C, the heat treatment applied to coated superalloy parts. Standard creep specimens 12.7 mm long and 3.2 mm in diameter were then machined. Constant load creep-rupture tests were performed in air and vacuum at 650, 850, and 1050 C and various initial stresses. In addition, some specimens were preoxidized at 1050 C for 100 h prior to testing. Results are briefly discussed.

Hebsur, M. G.↗

Aperture synthesis CO observations of the inner disk of NGC 1068

NGC 1068 is probably the nearest galaxy with both a high rate of star formation and a high luminosity active nucleus. About one-half of the total IR luminosity originates in a disk approximately 30 seconds in size, which has been taken as evidence for a high rate of massive star formation (Telesco et al. 1984). Previous CO line observations have shown the existence of a ring at the outer boundary of the inner disk (Myers and Scoville 1987). Although these observations have proved that this ring is extremely rich in molecular gas (as suggested by Scoville et al. 1983), the angular resolution (approx. 6 seconds) was not high enough to resolve its structure. New aperture synthesis observations of the CO (J=1 to 0) emission in the inner disk of NGC 1068 have been carried out with the Owens Valley Radio Observatory (OVRO) mm Interferometer. The new receivers installed for the 88/89 season have allowed researchers to obtain a high sensitivity map of the CO emission. The molecular cloud ring has been resolved and continuum as well as line emission from the nucleus of the galaxy have been detected.

Planesas, P.↗

Evolution of the Lunar Receiving Laboratory to the Astromaterial Sample Curation Facility: Technical Tensions Between Containment and Cleanliness, Between Particulate and Organic Cleanliness

The Lunar Receiving Laboratory (LRL) was planned and constructed in the 1960s to support the Apollo program in the context of landing on the Moon and safely returning humans. The enduring science return from that effort is a result of careful curation of planetary materials. Technical decisions for the first facility included sample handling environment (vacuum vs inert gas), and instruments for making basic sample assessment, but the most difficult decision, and most visible, was stringent biosafety vs ultra-clean sample handling. Biosafety required handling of samples in negative pressure gloveboxes and rooms for containment and use of sterilizing protocols and animal/plant models for hazard assessment. Ultra-clean sample handling worked best in positive pressure nitrogen environment gloveboxes in positive pressure rooms, using cleanable tools of tightly controlled composition. The requirements for these two objectives were so different, that the solution was to design and build a new facility for specific purpose of preserving the scientific integrity of the samples. The resulting Lunar Curatorial Facility was designed and constructed, from 1972-1979, with advice and oversight by a very active committee comprised of lunar sample scientists. The high precision analyses required for planetary science are enabled by stringent contamination control of trace elements in the materials and protocols of construction (e.g., trace element screening for paint and flooring materials) and the equipment used in sample handling and storage. As other astromaterials, especially small particles and atoms, were added to the collections curated, the technical tension between particulate cleanliness and organic cleanliness was addressed in more detail. Techniques for minimizing particulate contamination in sample handling environments use high efficiency air filtering techniques typically requiring organic sealants which offgas. Protocols for reducing adventitious carbon on sample handling surfaces often generate particles. Further work is needed to achieve both minimal particulate and adventitious carbon contamination. This paper will discuss these facility topics and others in the historical context of nearly 50 years' curation experience for lunar rocks and regolith, meteorites, cosmic dust, comet particles, solar wind atoms, and asteroid particles at Johnson Space Center.

Allton, J. H.↗

Multifrequency dial sensing of the atmospheric gaseous constituents using the first and second harmonics of a tunable CO2 laser radiation

The results of field measurements of concentration of some gaseous components of the atmosphere along the paths, in Sofia, Bulgaria, using a gas analyzer based on the use of a CO2 laser radiation frequency-doubled with ZnGeP2 monocrystals are presented. The gas analyzer is a traditional long path absorption meter. Radiation from the tunable CO2 laser of low pressure and from an additional He-Ne laser is directed to a colliminating hundredfold Gregori telescope with a 300 mm diameter of the principal mirror. The dimensions of the mirrors of a retroreflector 500 x 500 mm and a receiving telescope allow one to totally intercept the beam passed through the atmospheric layer under study and back.

Zuev, V. E.↗

The photons payload, G-494: A learning experience

PHOTONS (Photometric Thermospheric Oxygen Nightglow Study) is an optical remote sensing payload developed for Get Away Special (GAS) flight by the National Research Council of Canada. The device is extremely sensitive and is suitable for making measurements of low intensity, aeronomically generated atmospheric emissions in the nadir and the limb and of Shuttle ram glow. The unit uses a sealed canister and UV transmitting viewing ports. During the flight of STS 61-C, PHOTONS received one hour of operation and aeronomic observations were made. Good diagnostic data were obtained and the science part of the experiment malfunctioned. Post flight inspection revealed that the payload was in perfect working order except for total failure of the photomultiplier detectors. The experiment and the payload are described and the flight results are discussed along with the cause of the malfunctions. It is shown that enough was learned from the flight diagnostic data and about the cause of the malfunction to conclude that the engineering flight was successful and that subsequent flight of the PHOTONS payload will be productive.

Harris, F. R.↗