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At least 289 records · Page 16

AmeriFlux FLUXNET-1F US-xHA NEON Harvard Forest (HARV)

This is the AmeriFlux Management Project (AMP) created FLUXNET-1F version of the carbon flux data for the site US-xHA NEON Harvard Forest (HARV). This is the FLUXNET version of the carbon flux data for the site US-xHA NEON Harvard Forest (HARV) produced by applying the standard ONEFlux (1F) software. Site Description - The Harvard Forest site is comprised of 3,750 acres of land and multiple research facilities; it is the core NEON site for the Northeast region. Harvard Forest is a department of the Faculty of Arts and Sciences of Harvard University. Representative habitats at Harvard Forest include northern, transition, and central forests; marshes, swamps, and conifer-dominated bogs; and forest plantations. Since its inception in 1907, research and education have been the focus of Harvard Forest: the original purpose was to develop a field laboratory for students, a research center in forestry and related disciplines, and a demonstration of practical sustained forestry. Since 1988, Harvard Forest has been a Long-Term Ecological Research site, funded by the National Science Foundation to conduct integrated, long-term studies of forest dynamics.

Network), NEON (National Ecological Observatory↗

Understanding large‐scale, complex, human–environmental processes: a framework for social–ecological observatories

There is a growing consensus that transdisciplinary environmental networks are required to generate knowledge of the dynamics of coupled human–natural systems ( CHAN s) and to assess societal and policy consequences of complex environmental issues. Few of the existing large environmental observatory networks collect much, if any, data on the CHAN s coupling mechanisms, reaffirming the need to develop observing approaches and structures that address the interoperability of the diverse data being obtained from these systems. We review the characteristics of existing environmental observatories and similar initiatives used to monitor CHAN s, and identify opportunities for interoperability among them. From a synthesis of the characteristics of environmental networks representing a wide spectrum of research approaches and applications, a conceptual framework of a typical Social–Ecological Observatory ( SEO ) is presented to support long‐term, multi‐scaled CHAN s observations through a common set of principles for organizing the observatories. Finally, we identify three areas of emphasis where progress is needed to support the use and application of SEO s.

Bourgeron, Patrick↗

Multi‐Omics Analyses Reveal Divergent Molecular Mechanisms Underlying Plant Biomass Conversion by Five Fungi

Fungal plant biomass conversion (FPBC) is of great importance to the global carbon cycle and has been increasingly applied for the production of biofuel and biochemicals from lignocellulose. However, the comprehensive understanding of relevant molecular mechanisms in different fungi remains challenging. Here, we comparatively analyzed the transcriptome, proteome and metabolome profile of four ascomycetes and one basidiomycete fungi during their growth on two common agricultural feedstocks (soybean hulls and corn stover). We revealed strong time‐, substrate‐ and species‐specific responses at multi‐omics levels for the tested fungi, highlighting species‐specific carbon utilization approaches and evolutionary adaptation to environmental niches. Notably, a remarkable expressional diversity of lignocellulose degrading enzymes, sugar transporter and metabolic genes, as well as industrially relevant metabolites were identified across different fungi and cultivation conditions. The findings improves our understanding of complex molecular networks underlying FPBC and fungal ecological roles, offering novel insights that can guide future genetic engineering of fungi for valorization of agriculture waste into value‐added bioproducts.

CAZy↗

Nonequilibrium physics in biology

Life is characterized by a myriad of complex dynamic processes allowing organisms to grow, reproduce, and evolve. Physical approaches for describing systems out of thermodynamic equilibrium have been increasingly applied to living systems, which often exhibit phenomena not found in those traditionally studied in physics. Spectacular advances in experimentation during the last decade or two, for example, in microscopy, single-cell dynamics, in the reconstruction of subcellular and multicellular systems outside of living organisms, and in high throughput data acquisition, have yielded an unprecedented wealth of data on cell dynamics, genetic regulation, and organismal development. These data have motivated the development and refinement of concepts and tools to dissect the physical mechanisms underlying biological processes. Notably, landscape and flux theory as well as active hydrodynamic gel theory have proven useful in this endeavor. Together with concepts and tools developed in other areas of nonequilibrium physics, significant progress has been made in unraveling the principles underlying efficient energy transport in photosynthesis, cellular regulatory networks, cellular movements and organization, embryonic development and cancer, neural network dynamics, population dynamics and ecology, as well as aging, immune responses, and evolution. In this paper, recent advances in nonequilibrium physics are reviewd and their application to biological systems is surveyed. Many of these results are expected to be important cornerstones as the field continues to build our understanding of life.

59 BASIC BIOLOGICAL SCIENCES↗

SoDaH: the SOils DAta Harmonization database, an open-source synthesis of soil data from research networks, version 1.0

Data collected from research networks present opportunities to test theories and develop models about factors responsible for the long-term persistence and vulnerability of soil organic matter (SOM). Synthesizing datasets collected by different research networks presents opportunities to expand the ecological gradients and scientific breadth of information available for inquiry. Synthesizing these data is challenging, especially considering the legacy of soil data that have already been collected and an expansion of new network science initiatives. To facilitate this effort, here we present the SOils DAta Harmonization database (SoDaH; https://lter.github.io/som-website, last access: 22 December 2020), a flexible database designed to harmonize diverse SOM datasets from multiple research networks. SoDaH is built on several network science efforts in the United States, but the tools built for SoDaH aim to provide an open-access resource to facilitate synthesis of soil carbon data. Moreover, SoDaH allows for individual locations to contribute results from experimental manipulations, repeated measurements from long-term studies, and local- to regional-scale gradients across ecosystems or landscapes. Finally, we also provide data visualization and analysis tools that can be used to query and analyze the aggregated database. The SoDaH v1.0 dataset is archived and available at https://doi.org/10.6073/pasta/9733f6b6d2ffd12bf126dc36a763e0b4 (Wieder et al., 2020).

54 ENVIRONMENTAL SCIENCES↗

Genetic correlation network prediction of forest soil microbial functional organization

Soil ecological functions are largely determined by the activities of soil microorganisms, which, in turn, are regulated by relevant interactions between genes and their corresponding pathways. Therefore, the genetic network can theoretically elucidate the functional organization that supports complex microbial community functions, although this has not been previously attempted. We generated a genetic correlation network based on 5421 genes derived from metagenomes of forest soils, identifying 7191 positive and 123 negative correlation relationships. This network consisted of 27 clusters enriched with sets of genes within speci fi c functions, represented with corresponding cluster hubs. The clusters revealed a hierarchical architecture, re fl ecting the functional organization in the soil metagenomes. Positive correlations mapped functional associations, whereas negative correlations often mapped regulatory processes. The potential functions of uncharacterized genes were predicted based on the functions of located clusters. The global genetic correlation network highlights the functional organization in soil metagenomes and provides a resource for predicting gene functions. We anticipate that the genetic correlation network may be exploited to comprehensively decipher soil microbial commu

54 ENVIRONMENTAL SCIENCES↗

Relationship between forest clearing and biophysical factors in tropical environments: Implications for the design of a forest change monitoring approach

The relationship between forest clearing, biophysical factors (e.g, ecological zones, slope gradient, soils), and transportation network in Costa Rica was analyzed. The location of forested areas at four reference datas (1940, 1950, 1961, and 1977) as derived from aerial photography and LANDSAT MSS data was digitilized and entered into a geographically-referenced data base. Ecological zones as protrayed by the Holdridge Life Zone Ecology System, and the location of roads and railways were also digitized from maps of the entire country as input to the data base. Information on slope gradient and soils was digitized from maps of a 21,000 square kilometer area. The total area of forest cleared over four decades are related to biophysical factors was analyzed within the data base and deforestation rates and trends were tabulated. The relatiohship between forest clearing and ecological zone and the influence of topography, sils, and transportation network are presented and discussed.

Sader, S. A.↗

AmeriFlux US-xBL NEON Blandy Experimental Farm (BLAN)

This is the AmeriFlux version of the carbon flux data for the site US-xBL NEON Blandy Experimental Farm (BLAN). Site Description - The Blandy Experimental Farm contains several land use types typically found in rural-suburban landscapes. This mix of land use types is typical and representative in the Middle Atlantic Domain. This site will be under increasing ecological pressure from urbanization within the rapidly growing megapolitan area. The amount of land cover and associated ecosystem processes in each of these land use types is expected to change over time.

Network), NEON (National Ecological Observatory↗

AmeriFlux FLUXNET-1F US-xBL NEON Blandy Experimental Farm (BLAN)

This is the AmeriFlux Management Project (AMP) created FLUXNET-1F version of the carbon flux data for the site US-xBL NEON Blandy Experimental Farm (BLAN). This is the FLUXNET version of the carbon flux data for the site US-xBL NEON Blandy Experimental Farm (BLAN) produced by applying the standard ONEFlux (1F) software. Site Description - The Blandy Experimental Farm contains several land use types typically found in rural-suburban landscapes. This mix of land use types is typical and representative in the Middle Atlantic Domain. This site will be under increasing ecological pressure from urbanization within the rapidly growing megapolitan area. The amount of land cover and associated ecosystem processes in each of these land use types is expected to change over time.

Network), NEON (National Ecological Observatory↗

Islands Within Islands: Bacterial Phylogenetic Structure and Consortia in Hawaiian Lava Caves and Fumaroles

Lava caves, tubes, and fumaroles in Hawai‘i present a range of volcanic, oligotrophic environments from different lava flows and host unexpectedly high levels of bacterial diversity. These features provide an opportunity to study the ecological drivers that structure bacterial community diversity and assemblies in volcanic ecosystems and compare the older, more stable environments of lava tubes, to the more variable and extreme conditions of younger, geothermally active caves and fumaroles. Using 16S rRNA amplicon-based sequencing methods, we investigated the phylogenetic distinctness and diversity and identified microbial interactions and consortia through co-occurrence networks in 70 samples from lava tubes, geothermal lava caves, and fumaroles on the island of Hawai‘i. Our data illustrate that lava caves and geothermal sites harbor unique microbial communities, with very little overlap between caves or sites. We also found that older lava tubes (500–800 yrs old) hosted greater phylogenetic diversity (Faith's PD) than sites that were either geothermally active or younger (<400 yrs old). Geothermally active sites had a greater number of interactions and complexity than lava tubes. Average phylogenetic distinctness, a measure of the phylogenetic relatedness of a community, was higher than would be expected if communities were structured at random. This suggests that bacterial communities of Hawaiian volcanic environments are phylogenetically over-dispersed and that competitive exclusion is the main driver in structuring these communities. This was supported by network analyses that found that taxa (Class level) co-occurred with more distantly related organisms than close relatives, particularly in geothermal sites. Network “hubs” (taxa of potentially higher ecological importance) were not the most abundant taxa in either geothermal sites or lava tubes and were identified as unknown families or genera of the phyla, Chloroflexi and Acidobacteria. These results highlight the need for further study on the ecological role of microbes in caves through targeted culturing methods, metagenomics, and long-read sequence technologies.

59 BASIC BIOLOGICAL SCIENCES↗

Metabolic capability and in situ activity of microorganisms in an oil reservoir

Background: Microorganisms have long been associated with oxic and anoxic degradation of hydrocarbons in oil reservoirs and oil production facilities. While we can readily determine the abundance of microorganisms in the reservoir and study their activity in the laboratory, it has been challenging to resolve what microbes are actively participating in crude oil degradation in situ and to gain insight into what metabolic pathways they deploy. Results: Here, we describe the metabolic potential and in situ activity of microbial communities obtained from the Jiangsu Oil Reservoir (China) by an integrated metagenomics and metatranscriptomics approach. Almost complete genome sequences obtained by differential binning highlight the distinct capability of different community members to degrade hydrocarbons under oxic or anoxic condition. Transcriptomic data delineate active members of the community and give insights that Acinetobacter species completely oxidize alkanes into carbon dioxide with the involvement of oxygen, and Archaeoglobus species mainly ferment alkanes to generate acetate which could be consumed by Methanosaeta species. Furthermore, nutritional requirements based on amino acid and vitamin auxotrophies suggest a complex network of interactions and dependencies among active community members that go beyond classical syntrophic exchanges; this network defines community composition and microbial ecology in oil reservoirs undergoing secondary recovery. Conclusion: Our data expand current knowledge of the metabolic potential and role in hydrocarbon metabolism of individual members of thermophilic microbial communities from an oil reservoir. The study also reveals potential metabolic exchanges based on vitamin and amino acid auxotrophies indicating the presence of complex network of interactions between microbial taxa within the community.

59 BASIC BIOLOGICAL SCIENCES↗

Ecology Based Decentralized Agent Management System

The problem of maintaining a desired number of mobile agents on a network is not trivial, especially if we want a completely decentralized solution. Decentralized control makes a system more r e bust and less susceptible to partial failures. The problem is exacerbated on wireless ad hoc networks where host mobility can result in significant changes in the network size and topology. In this paper we propose an ecology-inspired approach to the management of the number of agents. The approach associates agents with living organisms and tasks with food. Agents procreate or die based on the abundance of uncompleted tasks (food). We performed a series of experiments investigating properties of such systems and analyzed their stability under various conditions. We concluded that the ecology based metaphor can be successfully applied to the management of agent populations on wireless ad hoc networks.

Peysakhov, Maxim D.↗

AmeriFlux FLUXNET-1F US-xJE NEON Jones Ecological Research Center (JERC)

This is the AmeriFlux Management Project (AMP) created FLUXNET-1F version of the carbon flux data for the site US-xJE NEON Jones Ecological Research Center (JERC). This is the FLUXNET version of the carbon flux data for the site US-xJE NEON Jones Ecological Research Center (JERC) produced by applying the standard ONEFlux (1F) software. Site Description - This terrestrial relocatable field site is located in the Joseph Jones Ecological Research Center is an 11,000-hectare reserve located within the Lower Coastal Plains and Flatwoods areas in southern Georgia. The Jones site has been managed with low intensity, dormant-season prescribed fires for the past 75 years at a frequency of every 3-4 years.

Network), NEON (National Ecological Observatory↗

A Task-Optimized Neural Network Replicates Human Auditory Behavior, Predicts Brain Responses, and Reveals a Cortical Processing Hierarchy

A core goal of auditory neuroscience is to build quantitative models that predict cortical responses to natural sounds. Reasoning that a complete model of auditory cortex must solve ecologically relevant tasks, we optimized hierarchical neural networks for speech and music recognition. The best-performing network contained separate music and speech pathways following early shared processing, potentially replicating human cortical organization. The network performed both tasks as well as humans and exhibited human-like errors despite not being optimized to do so, suggesting common constraints on network and human performance. The network predicted fMRI voxel responses substantially better than traditional spectrotemporal filter models throughout auditory cortex. It also provided a quantitative signature of cortical representational hierarchy—primary and non-primary responses were best predicted by intermediate and late network layers, respectively. Here the results suggest that task optimization provides a powerful set of tools for modeling sensory systems.

59 BASIC BIOLOGICAL SCIENCES↗

Mechanism Across Scales: A Holistic Modeling Framework Integrating Laboratory and Field Studies for Microbial Ecology

Over the last century, leaps in technology for imaging, sampling, detection, high-throughput sequencing, and -omics analyses have revolutionized microbial ecology to enable rapid acquisition of extensive datasets for microbial communities across the ever-increasing temporal and spatial scales. The present challenge is capitalizing on our enhanced abilities of observation and integrating diverse data types from different scales, resolutions, and disciplines to reach a causal and mechanistic understanding of how microbial communities transform and respond to perturbations in the environment. This type of causal and mechanistic understanding will make predictions of microbial community behavior more robust and actionable in addressing microbially mediated global problems. To discern drivers of microbial community assembly and function, we recognize the need for a conceptual, quantitative framework that connects measurements of genomic potential, the environment, and ecological and physical forces to rates of microbial growth at specific locations. We describe the Framework for Integrated, Conceptual, and Systematic Microbial Ecology (FICSME), an experimental design framework for conducting process-focused microbial ecology studies that incorporates biological, chemical, and physical drivers of a microbial system into a conceptual model. Through iterative cycles that advance our understanding of the coupling across scales and processes, we can reliably predict how perturbations to microbial systems impact ecosystem-scale processes or vice versa. We describe an approach and potential applications for using the FICSME to elucidate the mechanisms of globally important ecological and physical processes, toward attaining the goal of predicting the structure and function of microbial communities in chemically complex natural environments.

59 BASIC BIOLOGICAL SCIENCES↗

A dataset of eco-evidence tools to inform early-stage environmental impact assessments of hydropower development

The datasets described herein provide the foundation for a decision support prototype (DSP) toolkit aimed at assisting stakeholders in determining evidence of which aspects of river ecosystems have been impacted by hydropower. The DSP toolkit and its application are presented and described in the article “Evidence-based indicator approach to guide preliminary environmental impact assessments of hydropower development” [1]. Development of the DSP and the output for decision support centralize around 42 river function indicators describing the dimensionality of river ecosystems through six main categories: biota and biodiversity, water quality, hydrology, geomorphology, land cover, and river connectivity. Three main tools are represented in the DSP: A science-based questionnaire (SBQ), an environmental envelope model (EEM), and a river function linkage assessment tool (RFLAT). The SBQ is a structured survey-style questionnaire whose objective is to provide evidence of which indicators have been impacted by hydropower. Based on a global literature review, 140 questions were developed from general hypotheses regarding the impacts of dams on rivers. The EEM is a model to predict the likelihood of hydropower impacting indicators based on a several variables. The intended use of the EEM is for situations of new hydropower development where results of the SBQ are incomplete or highly uncertain. The EEM was developed through the compilation of a dataset containing attributes of dams, reservoirs, and geospatial information on environmental concerns, which was combined with data on ecological indicators documented at those sites through literature review. The model operates through 247 “envelopes” and weighting factors, representing the individual effect of each variable on each indicator, all available through spreadsheets. Finally, the RFLAT is a tool to examine causal relationships amongst indicators. Inter-indicator relationships were hypothesized based on literature review and summarized into node and edge datasets to represent the structure of a graphical network. Bayes theorem was used estimate conditional probabilities of inter-indicator relationships based on the output of the SBQ. Nodes and edges were imported into R programming environment to visualize ecological indicator networks. The datasets can be expanded upon and enriched with more detailed questions for the SBQ, building upon the EEM with to develop more sophisticated models, and identifying new relationships for the RFALT. Additionally, once the tools are applied to numerous hydropower developments, the output of the tools (e.g. evidence of impacted indicators) becomes a very useful dataset for meta-analyses of hydropower impacts.

13 HYDRO ENERGY↗

The nitrogen gap in soil health concepts and fertility measurements

Soil nitrogen (N) often limits productivity in agroecosystems, prompting fertilizer applications that increase crop yields but can degrade the environment. Nitrogen's dual role in both productivity and environmental quality should center it in soil health frameworks. We use recent evidence to argue that N availability is an emergent property of the integrated soil biogeochemical system and is strongly influenced by plant traits and their interactions with microbes and minerals. Building upon this, we theorize that the sources of plant and microbial N shift across soil health gradients, from inorganic N dependence in ecologically simple systems with poor soil health to a highly networked supply of organic N in healthy soils; ergo, investments in soil health should increase ecological complexity and the pathways by which plants can access N, leading to more resilient nutrient supplies and yields in a variable climate. However, current N assessment methods derive from a historical emphasis on inorganic N pool sizes and are unable to capture the shifting drivers of N availability across soil health gradients. We highlight the need to better understand the plant-microbial-mineral interactions that regulate bioavailable N as a first step to improving our ability to measure it. We conclude it will be necessary to harness agroecosystem complexity, account for plant and microbial drivers, and carefully integrate external N inputs into soils' internal N network to expand the routes by which N from organic pools can be made bioavailable. Finally, by emphasizing N in soil health concepts, we argue that researchers can accelerate advances in N use efficiency and resiliency.

60 APPLIED LIFE SCIENCES↗