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At least 271 records · Page 15

Simulated nitrogen deposition and precipitation events alter microbial carbon cycling during early stages of litter decomposition

Plant litter decomposition is a major nutrient input to terrestrial ecosystems that is primarily driven by microorganisms. Litter quality is considered a key drive of decomposition; however, human-induced global disturbance like nitrogen deposition and increasing extreme precipitation events will shift nutrient availability during litter decomposition. Little is known about how shifting nutrient availability will impact dissolved organic matter concentrations and microbially driven carbon cycling that are critical to soil organic matter formation. This study investigated the effect of simulated nitrogen deposition and repeated precipitation events on microbially driven carbon flow during short-term litter decomposition using a ‘common garden’ experiment with microcosms containing sand and blue grama grass litter inoculated with different microbial communities. Overall, nitrogen deposition decoupled respiration and dissolved organic carbon (DOC) by increasing respiration and not affecting DOC concentrations. Moreover, nitrogen deposition had no effect on microbial carbon use efficiency (CUE). Repeated simulated precipitation events significantly increased DOC concentrations, decreased microbial CUE, increased the microbial metabolic quotient (qCO2), and altered microbial composition and diversity. These findings highlight the complex interactions and responses of surface litter decomposers to shifting nutrient availability and contradicts previous findings that nitrogen deposition will increase soil carbon sequestration from a larger supply of DOC and reduced respiration.

59 BASIC BIOLOGICAL SCIENCES↗

Distinct xylem responses to acute vs prolonged drought in pine trees

Increasing dryness challenges trees’ ability to maintain water transport to the leaves. Most plant hydraulics models use a static xylem response to water stress. Yet, in reality, lower soil moisture and warmer temperatures during growing seasons feed back onto xylem development. In turn, adjustments to water stress in the newly built xylem influence future physiological responses to droughts. In this study, we investigate the annual variation of anatomical traits in branch xylem in response to different soil and atmospheric moisture conditions and tree stress levels, as indicated by seasonal predawn leaf water potential (??L,pd). We used a 6-year field experiment in southwestern USA with three soil water treatments applied to Pinus edulis Engelm trees—ambient, drought (45% rain reduction) and irrigation (15–35% annual water addition). All trees were also subject to a natural 1-year acute drought (soil and atmospheric) that occurred during the experiment. The irrigated trees showed only moderate changes in anatomy-derived hydraulic traits compared with the ambient trees, suggesting a generally stable, well-balanced xylem structure under unstressed conditions. The artificial prolonged soil drought increased hydraulic efficiency but lowered xylem construction costs and decreased tracheid implosion safety ((t/b)2), suggesting that annual adjustments of xylem structure follow a safety–efficiency trade-off. The acute drought plunged hydraulic efficiency across all treatments. The combination of acute and prolonged drought resulted in vulnerable and inefficient new xylem, disrupting the stability of the anatomical trade-off observed in the rest of the years. The xylem hydraulic traits showed no consistent direct link to ??L,pd. In the future, changes in seasonality of soil and atmospheric moisture are likely to have a critical impact on the ability of P. edulis to acclimate its xylem to warmer climate. Furthermore, the increasing frequency of acute droughts might reduce hydraulic resilience of P. edulis by repeatedly creating vulnerable and less efficient anatomical structure.

Guerin, Marceau↗

Extending evolutionary forecasts across bacterial species

Improving evolutionary forecasting requires progressing from studying repeated evolution of a single genotype under identical conditions to formulating broad principles. These principles should enable predictions of how similar species will adapt to similar selective pressures. Evolve-and-resequence experiments with multiple species allow testing forecasts on different biological levels and elucidating the causes for failed predictions. Here, we show that forecasts for adaptation to static culture conditions can be extended to multiple species by testing previous predictions for Pseudomonas syringae and Pseudomonas savastanoi. In addition to sequence divergence, these species differ in their repertoire of biofilm regulatory genes and structural components. Consistent with predictions, both species repeatedly produced biofilm mutants with a wrinkly spreader phenotype. Predominantly, mutations occurred in the wsp operon, with less frequent promoter mutations near uncharacterized diguanylate cyclases. However, mutational patterns differed on the gene level, which was explained by a lack of conservation in relative fitness of mutants between more divergent species. The same mutation was the most frequent for both species suggesting that conserved mutation hotspots can increase parallel evolution. This study shows that evolutionary forecasts can be extended across species, but that differences in the genotype–phenotype–fitness map and mutational biases limit predictability on a detailed molecular level.

59 BASIC BIOLOGICAL SCIENCES↗

Zero-added-loss entanglement multiplexing using time-bin spectral shearing

High-quality quantum communications that enable important capabilities, such as distributed quantum computing and sensing, will require quantum repeaters for providing high-quality entanglement. To realize high-rate heralded entanglement for quantum repeaters, Chen et al . [Phys. Rev. Appl. 19 , 054029 (2023)] proposed a scheme for heralded-multiplexed generation of quasideterministic entangled photon pairs, called zero-added-loss multiplexing (ZALM). Here, we propose a design of the ZALM source using time-bin entanglement and spectral shearing. Additionally, we provide an analysis of experimentally relevant spectral-shearing parameters to optimize the spectral multiplexing. Moreover, we experimentally verify the compatibility of time-bin pulses and spectral shearing, as supported by observation of no appreciable phase shift when the same shearing is applied to both time bins. These results expand the benefits of applying a ZALM source to time-bin entanglement use cases. Moreover, more fully demonstrating time-bin and spectral shearing compatibility clears a path toward a broader use of spectral shearing that provides a deterministic frequency shift of high utility.

entanglement production↗

Sensor-Assisted Fault Mitigation in Quantum Computation

We propose a method for assisting fault mitigation in quantum computation through the use of sensors, co-located near physical qubits. Specifically, we consider using transition edge sensors co-located on silicon substrates hosting superconducting qubits to monitor for energy injection from ionizing radiation that has been demonstrated to increase decoherence in transmon qubits. We generalize from these two physical device concepts and explore the potential advantages of co-located sensors to assist fault mitigation in quantum computation. In the simplest scheme, co-located sensors beneficially assist rejection of calculations potentially affected by environmental disturbances. Investigating the potential computational advantage further required development of an extension to the standard formulation of quantum error correction. In a specific case the standard three-qubit, bit flip quantum correction code, we show that given a 20% overall error probability per qubit, approximately 90% of repeated calculation attempts are correctable. However, when sensor-detectable errors account for 45% of overall error probability, the use of colocated sensors uniquely associated with independent qubits, boosts the fraction of correct final-state calculations to 96% at the cost of rejecting 7% of repeated calculation attempts.

Orrell, John L.↗

Detecting dark matter substructures on small scales with fast radio bursts

The matter power spectrum is only weakly constrained on subgalactic scales, while physics beyond the Standard Model can leave unique imprints, especially on sub-parsec scales. We propose measuring the arrival-time difference of fast radio bursts (FRBs) along two adjacent sightlines as a new probe to dark matter substructures on scales down to ∼ 1 AU . We discuss two observational scenarios in which it may be possible to place interesting constraints on such models through the monitoring of repeating FRB sources: (i) By sending radio receivers to space to form a baseline of tens of AU or more and measuring the temporal variation of the arrival-time difference between receivers. (ii) By measuring the temporal variation of the arrival-time difference between two lensed images of one strongly lensed repeater. In both scenarios, obtaining interesting constraints requires correlating the voltage time series to measure the radio signal arrival time to sub-nanosecond precision. We find that two radio dishes separated by 20 AU may be sensitive to the enhancement of small-scale structures at ∼ 10 - 8 M ⊙ masses in the QCD axion dark matter scenario, or from an early epoch of matter domination with a reheating temperature up to 60 MeV. Other dark matter models, such as those composed of ∼ 10 - 13 M ⊙ primordial black holes produced during inflation, would also be probed by this method. We further show that a strong lensing situation of multiple images provides an equivalent ∼ 2000 AU ( σ v / 10 3 km s - 1 ) ( δ t / 10 yr ) baseline, for a typical velocity of dark matter substructures σ v and an observational time span δ . This is much more sensitive, but with the uncertainty that intervening decoherence from the interstellar medium may degrade the timing precision, and that spatial variation in the FRB emission spot may result in confounding signals. We show that the lensing magnifications of Type Ia supernovae constrain a similar quantity to such FRB timing, with present limits being equivalent to ruling out the same parameter space that would be probed by a 0.14 AU baseline.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗

Quantifying local rearrangements in three-dimensional granular materials: Rearrangement measures, correlations, and relationship to stresses

Quantifying the ways in which local particle rearrangements contribute to macroscopic plasticity is one of the fundamental pursuits of granular mechanics and soft matter physics. Here we examine local rearrangements that occur naturally during the deformation of three samples of 3D granular materials subjected to distinct boundary conditions by employing in situ x-ray measurements of particle-resolved structure and stress. We focus on five distinct rearrangement measures, their statistics, interrelationships, contributions to macroscopic deformation, repeatability, and dependence on local structure and stress. Our most significant findings are that local rearrangements (1) are correlated on a scale of three to four particle diameters, (2) exhibit volumetric strain-shear strain and nonaffine displacement-rotation coupling, (3) exhibit correlations that suggest either rearrangement repeatability or that rearrangements span multiple steps of incremental sample strain, and (4) show little dependence on local stress but correlate with quantities describing local structure, such as porosity. Our results are presented in the context of relevant plasticity theories and are consistent with recent findings suggesting that local structure may play at least as important of a role as local stress in determining the nature of local rearrangements.

58 GEOSCIENCES↗

A functional modeling approach for quality assurance in metal additive manufacturing

Purpose: Due to the complexity of and variations in additive manufacturing (AM) processes, there is a level of uncertainty that creates critical issues in quality assurance (QA), which must be addressed by time-consuming and cost-intensive tasks. This deteriorates the process repeatability, reliability and part reproducibility. So far, many AM efforts have been performed in an isolated and scattered way over several decades. Here, a systematically integrated holistic view is proposed to achieve QA for AM. Design/methodology/approach: A systematically integrated view is presented to ensure the predefined part properties before/during/after the AM process. It consists of four stages, namely, QA plan, prospective validation, concurrent validation and retrospective validation. As a foundation for QA planning, a functional workflow and the required information flows are proposed by using functional design models: Icam DEFinition for Function Modeling. Findings: The functional design model of the QA plan provides the systematically integrated view that can be the basis for inspection of AM processes for the repeatability and qualification of AM parts for reproducibility. Research limitations/implications: A powder bed fusion process was used to validate the feasibility of this QA plan. Feasibility was demonstrated under many assumptions; real validation is not included in this study. Social implications: This study provides an innovative and transformative methodology that can lead to greater productivity and improved quality of AM parts across industries. Furthermore, the QA guidelines and functional design models provide the foundation for the development of a QA architecture and management system. Originality/value: This systematically integrated view and the corresponding QA plan can pose fundamental questions to the AM community and initiate new research efforts in the in-situ digital inspection of AM processes and parts.

36 MATERIALS SCIENCE↗

Novel Technique for Developing Linearized Convex System Models from Experimentally Derived Data

This paper presents a novel technique for generating a convex system model from an experimentally derived data set which features variance among repeated measurements. The convex system model developed as a test case characterizes the dynamic system losses of a vanadium redox flow battery as a function of the active power output and the battery state of charge. The technique hinges on a pre-cleaning via clustering procedure which precedes the formation of a planar convex hull comprised of triangular simplices. The clustering procedure efficiently reduces the experimental data set while mitigating variance among repeated measurements and removes outliers. Ultimately, the lower evelope of the planar convex hull serves as the desired convex system model. The proposed technique reduces systematic model error which is otherwise present when directly developing a planar convex hull model based on an unreduced data set.

24 POWER TRANSMISSION AND DISTRIBUTION↗

Determination of Series Resistance in CdSeTe/CdTe Solar Cells by the Jsc–Voc Method

While commonly used in Si solar cell characterization, the Suns–Voc method of determining series resistance (Rs) is rarely used for CdTe and its alloys. However, it is advantageous relative to the slope method—commonly used in the CdTe community—because it measures series resistance at the maximum power point, at which a solar cell operates. Using the self-consistent predecessor to Suns–Voc, Jsc –Voc, , we determine Rs of two different CdSeTe/CdTe solar cell structures to be 0.96 and 2.72 Ωcm2, with repeated measurements showing little deviation

13 HYDRO ENERGY↗

Modifications to the JET Shattered Pellet Injector to Optimize Disruption Mitigation Experiments for Supporting ITER’s DMS Design

Shattered pellet injection (SPI) experiments on Joint European Torus (JET) are an important element in determining the physics basis for mitigating disruptions in ITER. Here, the initial design of the JET SPI system included three barrels to produce pellets with diameters of 4.5, 8.1, and 12.5 mm. The variability of the pellet speed by operating with and without a mechanical punch was limited and led to poor pellet integrity, so the mechanical punch was removed. Fragment size distribution is a function of pellet speed and the desire to change the resulting fragment size distribution was not originally a requirement. After the first set of SPI experiments on JET, different pellet sizes were considered to enable dual injection experiments with identical pellet diameters. It was also determined that speed control is necessary to improve experimental repeatability and to determine how the fragment size distribution impacts mitigation performance. Two barrels were fabricated to form pellets of 10 mm diameter and a third to form an 8.1 mm diameter pellet. New propellant valves were also fabricated and characterized to improve repeatability and overall performance. To have fine control of pellet speed, inserts to reduce the breech volume were fabricated and installed. Laboratory testing was conducted to ensure pellet release and provide a comparison of propellant gas delivered versus pellet speeds for a range of pellet types and mixtures. This article will also discuss how lessons learned from the JET SPI modifications can be applied to other SPI systems, such as the ITER SPI system, as controlling pellet release with the least amount of propellant gas is essential for optimal SPI effectiveness.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗

InterQnet: A Heterogeneous Full-Stack Approach to Co-Designing Scalable Quantum Networks

Quantum communications have progressed significantly, moving from a theoretical concept to small-scale experiments to recent metropolitan-scale demonstrations. As the technology matures, it is expected to revolutionize quantum computing in much the same way that classical networks revolutionized classical computing. Quantum communications will also enable breakthroughs in quantum sensing, metrology, and other areas. However, scalability has emerged as a major challenge, particularly in terms of the number and heterogeneity of nodes, the distances between nodes, the diversity of applications, and the scale of user demand. This article describes InterQnet, a multidisciplinary project that advances scalable quantum communications through a comprehensive approach that improves devices, error handling, and network architecture. InterQnet has a two-pronged strategy to address scalability challenges: InterQnet-Achieve focuses on practical realizations of heterogeneous quantum networks by building and then integrating first-generation quantum repeaters with error mitigation schemes and centralized automated network control systems. The resulting system will enable quantum communications between two heterogeneous quantum platforms through a third type of platform operating as a repeater node. InterQnet-Scale focuses on a systems study of architectural choices for scalable quantum networks by developing forward-looking models of quantum network devices, advanced error correction schemes, and entanglement protocols. Here, we report our current progress toward achieving our scalability goals.

Chung, Joaquin [Argonne] (ORCID:0000000173833810)↗

A genomic region associated with iteroparous spawning phenology is linked with age‐at‐maturity in female steelhead trout

Abstract Age‐at‐maturity and iteroparity are two life history variations of steelhead trout ( Oncorhynchus mykiss ) that are believed to increase population resilience and stability. While repeat‐spawning individuals are thought to have historically made up a substantial portion of the reproductive population in the Columbia River and the majority of females still attempt outmigration as kelts, return rates of repeat‐spawner are low throughout the basin and below 1% for the furthest migrating stocks. Notably, outmigrating adults exhibit variation in rematuration phenology, displaying either “consecutive” (reproduce immediately the following season) or “skip” (delay spawning for future seasons) spawning patterns. Here, we use low coverage whole genome sequencing of consecutive versus skip spawning female Columbia River steelhead from two populations to test for genomic differences between these two iteroparous phenotypes. We identified genomic regions on several chromosomes which were associated with the phenology of iteroparity, including a region on chromosome 25 containing two genes, estradiol receptor beta ( ER β) and glycoprotein hormone beta‐5 ( GPHB5 ), which, in mammals, are estrogen‐sensitive and expressed in reproductive tissues. Allele frequencies in this ER β/ GPHB5 region differed among female steelhead of different age at maturity, but not males. These genes also shared an island of linkage disequilibrium with the SIX6 gene, 600Kbp away on the same chromosome, a region of known association with age‐at‐maturity. These observations contribute to growing evidence that age‐at‐maturity and the phenology of iteroparity are determined by overlapping physiological processes and genetic pathways.

Willis, Stuart↗

Low-intensity frequent fires in coniferous forests transform soil organic matter in ways that may offset ecosystem carbon losses

The impact of shifting disturbance regimes on soil carbon (C) storage is a key uncertainty in global change research. Wildfires in coniferous forests are becoming more frequent in many regions, potentially causing large C emissions. Repeated low-intensity prescribed fires can mitigate wildfire severity, but repeated combustion may decrease soil C unless compensatory responses stabilize soil organic matter. Here, we tested how 30 years of decadal prescribed burning affected C and nitrogen (N) in plants, detritus, and soils in coniferous forests in the Sierra Nevada mountains, USA. Tree basal area and litter stocks were resilient to fire, but fire reduced forest floor C by 77% (-36.4 Mg C/ha). In mineral soils, fire reduced C that was free from minerals by 41% (-4.4 Mg C/ha) but not C associated with minerals, and only in depths ≤ 5 cm. Fire also transformed the properties of remaining mineral soil organic matter by increasing the proportion of C in a pyrogenic form (from 3.2% to 7.5%) and associated with minerals (from 46% to 58%), suggesting the remaining soil C is more resistant to decomposition. Laboratory assays illustrated that fire reduced microbial CO2 respiration rates by 55% and the activity of eight extracellular enzymes that degrade cellulosic and aromatic compounds by 40–66%. Lower decomposition was correlated with lower inorganic N (-49%), especially ammonium, suggesting N availability is coupled with decomposition. The relative increase in forms of soil organic matter that are resistant to decay or stabilized onto mineral surfaces, and the associated decline in decomposition suggest that low-intensity fires may promote mineral soil C storage in pools with long mean residence times in coniferous forests.

54 ENVIRONMENTAL SCIENCES↗

Sustained Ability of a Natural Microbial Community to Remove Nitrate from Groundwater

Abstract Microbial‐mediated nitrate removal from groundwater is widely recognized as the predominant mechanism for nitrate attenuation in contaminated aquifers and is largely dependent on the presence of a carbon‐bearing electron donor. The repeated exposure of a natural microbial community to an electron donor can result in the sustained ability of the community to remove nitrate; this phenomenon has been clearly demonstrated at the laboratory scale. However, in situ demonstrations of this ability are lacking. For this study, ethanol (electron donor) was repeatedly injected into a groundwater well (treatment) for six consecutive weeks to establish the sustained ability of a microbial community to remove nitrate. A second well (control) located upgradient was not injected with ethanol during this time. The treatment well demonstrated strong evidence of sustained ability as evident by ethanol, nitrate, and subsequent sulfate removal up to 21, 64, and 68%, respectively, as compared to the conservative tracer (bromide) upon consecutive exposures. Both wells were then monitored for six additional weeks under natural (no injection) conditions. During the final week, ethanol was injected into both treatment and control wells. The treatment well demonstrated sustained ability as evident by ethanol and nitrate removal up to 20 and 21%, respectively, as compared to bromide, whereas the control did not show strong evidence of nitrate removal (5% removal). Surprisingly, the treatment well did not indicate a sustained and selective enrichment of a microbial community. These results suggested that the predominant mechanism(s) of sustained ability likely exist at the enzymatic‐ and/or genetic‐levels. The results of this study demonstrated the in situ ability of a microbial community to remove nitrate can be sustained in the prolonged absence of an electron donor.

54 ENVIRONMENTAL SCIENCES↗

Structure and sequence evolution in the pennycress ( Thlaspi arvense ) pangenome

Eukaryotic genomes harbor many forms of variation, including nucleotide diversity and structural polymorphisms, which experience natural selection and contribute to genome evolution and biodiversity. Harnessing this variation for agriculture hinges on our ability to detect, quantify, catalog, and deploy genetic diversity. Here, we explore seven complete genomes of the emerging biofuel crop pennycress ( Thlaspi arvense ) drawn from across the species' current genetic diversity to catalog variation in genome structure and content. Across this new pangenome resource, we find contrasting evolutionary modes in different genomic zones. Gene-poor, repeat-rich pericentromeric regions experience frequent rearrangements, including repeated centromere repositioning. By contrast, conserved gene-dense chromosome arms maintain large-scale synteny across accessions even in fast-evolving NOD-like receptor immune genes, where microsynteny breaks down across species, but gene cluster positioning macrosynteny is maintained. Our findings highlight that multiple elements of the genome experience dynamic evolution that conserves functional content on the chromosome scale but allows repositioning and presence–absence variation on a local scale. This diversity is invisible to classical reference-based strategies and highlights the strength and utility of pangenomic resources. These results provide a valuable case study of rapid genomic structural evolution within a species and powerful resources for crop development in an emerging biofuel crop.

Thlaspi arvense↗

Effect of Hydrogen on Steady-State and Transient Combustion Instability Characteristics

This paper examines the effects of steady-state and transient hydrogen enrichment on thermoacoustic instability in a model gas turbine combustor. Combustion instability, a feedback loop between flame heat release rate oscillations and combustor acoustics, is characterized in a swirl-stabilized flame operated at a range of hydrogen–natural gas fuel blends and heat rates. Measurements of combustor chamber pressure fluctuations and CH* chemiluminescence imaging are used to characterize instability at a range of operating conditions. Steady-state tests show that both mixture heat rate and hydrogen content affect system stability. At a given heat rate, higher levels of hydrogen result in unstable combustion. As heat rate increases, instability occurs at lower concentrations of hydrogen in the fuel. Transient operation was tested in two directions—instability onset and decay—and two hydrogen-addition times—a short time of 1 ms and a longer time of 4 s. Results show that instability onset processes, through the transient addition of hydrogen, are highly repeatable regardless of the timescale of hydrogen addition. Certain instability decay processes are less repeatable, resulting in cases that do not fully transition from unstable to stable combustion despite similar changes in hydrogen fuel flow rate. Flame behavior before, during, and after the transient is characterized using high-speed CH* chemiluminescence imaging. Analysis of the high-speed images shows changes in flame stabilization and dynamics during the onset and decay processes. Finally, the results of this study can have implications for systems that experience variations in fuel composition, particularly in light of growing interest in hydrogen as a renewable fuel.

42 ENGINEERING↗

REGULATORY TESTING AND POSTTEST ANALYSIS OF THE DPP-3 TYPE B SHIPPING CONTAINER FOR NCT AND HAC TESTS

The safe transportation of radioactive material using appropriately designed and certified transportation packages is important to protect the public and environment. Therefore, a comprehensive and rigorous system of packaging requirements are required. The purpose of this test protocol is to determine the effects of drop testing on package performance in a Type B shipping package (DPP-3). Required physical tests for the DPP-3 package include normal conditions of transport and hypothetical accident conditions test requirements. The preparation, regulatory testing, and post-test evaluation of a Type B shipping package tested to the 10 CFR 71.71 and 10 CFR 71.73 requirements will be presented. Six packages were tested and the responses when subjected to the HAC sequence of free drop, puncture, and thermal tests according to 10 CFR 71.73 were measured. The posttest helium leak test showed a leak rate less than 1 × 10-7 He-cc/sec which means that the containment boundary was maintained after the regulatory testing, therefore maintaining a leak tight environment. To determine the amount of change in the package performance measurements, statistical analyses were conducted using SPSS version 26. The null hypothesis of the testing was that there would not be a change in the measurements from pre to post-testing large enough to find statistical significance. Therefore, a finding of statistical significance indicated too much variability in the package design and was deemed an unfavorable finding. A repeated measures t-test was used to analyze the drum lids and CV torques. A one-way ANOVA was used to analyze height and diameter packages. Results of the repeated measures t-test indicated that all six containment vessel lid torques were statistically significant. However only three out of the six drum lid torques were statistically significant. Therefore, design of the lids caused more variability than necessary when subjected to the following drop tests: 1.) horizontal and cold; 2.) vertical lid down; and 3.) corner with lid up. Results of the one-way ANOVA analysis on height indicated that four out of the six CTUs were statistically significant. Thus, design of the CTUs caused more variability then necessary when subjected to the following drop tests: 1.) horizontal hot; 2.) horizontal cold; 3.) corner lid up; and 4.) corner lid down. Results of the one-way ANOVA analysis on diameter indicated that all six CTUs were statistically significant. Thus, design of the CTUs caused more variability then necessary when subjected to all of the tests. The DPP-3 design has been shown to be an acceptable design for the transport of radioactive materials. However, results of the statistical analysis reveal a possible improvement in package and test design to reduce variability in package performance measurement before and after testing.

Martinez, Oscar↗