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At least 271 records · Page 15

Fire Island Water Resources: Assessing Sediment Dynamics and Turbidity Changes Along Fire Island National Seashore Using Satellite Data

Fire Island National Seashore has experienced damaging effects as a result of coastal erosion. Erosion has become an increasingly damaging problem that has led to the destruction of park and community infrastructure, contributed to rising groundwater tables, and posed a serious threat to a globally rare holly maritime forest. Beach nourishment project efforts have been made to mitigate damage, but dredging is expensive and can be thwarted by high rates of erosion. The NASA DEVELOP team partnered with the National Park Service and Fire Island National Seashore and used imagery from Landsat 5 Thematic Mapper (TM), Landsat 8 Operational Land Imager (OLI), and Sentinel-2 MultiSpectral Instrument (MSI) to analyze turbidity and sediment dynamics through surface reflectance data from 2000-2021. Imagery was atmospherically corrected using Atmospheric Correction for OLI Lite (ACOLITE) and visualized in SeaWiFS Data Analysis Systems (SeaDAS). Additionally, shoreline change was analyzed using high-resolution imagery from WorldView-2 acquired from Maxar for pre-Hurricane Sandy on July 25th, 2010 and post-Hurricane Sandy on December 18th, 2019. The results of this analysis showed that turbidity is highest in the winter seasons. The shoreline analysis estimated total shoreline loss of about 62 acres on the ocean side, and 11 acres on the bay side of the island. These results will be used to better inform future partner-designed shoreline management projects in the face of further erosion and sea level rise.

Kelly Young↗

Space-Based Lidar Observations of the 3D Structure of the Earth System

Lidar provides precise measurements of the three-dimensional structure of the clouds, aerosols, ocean/land/snow/ice surfaces, as well as ocean subsurface. Lidar also provides unique information about physical propertiesof particulates in the atmosphere for both radiative transfer and air quality applications.In this talk, I will present an overview of our recent studies of aerosols, clouds, ocean and snow using space-based lidar measurements (e.g., LITE, CALIPSO and ICESat-2), such as classifications of aerosols and thermodynamics phase of clouds, cloud microphysical properties, snow depths and phytoplankton biomass. I will also introduce a new concept of 3D Earth system observations with data fusion though combined active/passive remote sensing and machine learning. The new concept aims to reveal vertical structure of the aerosols/clouds/surfaces/subsurface from passive sensors by taking advantage of lidar measurements to effectively resolve the vertical structureby unscrambling the highly convoluted multi-angle, spectral and polarization information from passive sensors and apply the knowledge to a large swath where lidar measurements are not available.

Ali Omar↗

CubeSat Active Thermal Control in Support of Advanced Payloads: The Active Thermal Architecture Project

The Active Thermal Architecture (ATA) is an advanced sub-1U Active Thermal Control technology (ATC) for high power payload support in 6U CubeSat form factors and above. The design utilizes a two-stage, single-phase mechanically pumped fluid loop coupled through a two-axis flexible rotary fluid hinge, to reject thermal power to a deployable tracking radiator. A COTS Ricor K508N cryocooler forms the second stage and provides cryogenic cooling to a custom Kevlar detector mount through a TMT pyrolytic graphene thermal strap. Passive vibration isolation and damping technologies prevent the transfer of jitter to the satellite systems. The ATA design utilizes state-of-the-art 3D fabrication techniques such as Ultrasonic Additive Manufacturing (UAM) to directly embed the working fluid channels into the HX, radiator, and CubeSat chassis allowing for the miniaturization and simplification of the ATA system into an integrated thermal control solution. This paper will focus on the design and ground-based characterization and qualification of the ATA system and provide performance metrics for its use as a thermal support subsystem for advanced infrared electro-optical CubeSat payloads. The ATA project is funded through a NASA Small Satellite Technology Program (SSTP) and is a partnership between the Center for Space Engineering at Utah State University and the Jet Propulsions Laboratory. The ATA active thermal control system has been raised to a TRL of 6 and hopes to provide payload support to advanced missions such as the SABER-Lite and JPL CIRAS projects.

Mok, Mason↗

The Psyche Planning Software Subsystem: Creating a Robust Toolset for a Discovery-class Mission

Psyche is a Discovery-class mission to the small metal-rich asteroid (16) Psyche, and is slated to launch in 2022. Psyche, like many missions, requires low-cost activity planning and sequence generation that serves as the backbone to overall uplink design. Such tools must be maintainable over long periods of operations, and powerful enough to solve complex issues that deep-space one-off missions encounter. In this paper we introduce cost-effective solutions that leverage inner- and open-source principles to meet a variety of common and novel use cases.The uplink process that was designed to meet these challenges is presented, as well as the data-flow through the high-level architecture of the planning software subsystem. The user-facing planning tools are described, particularly the Science Opportunity Analyzer, the Plan Editor, Psyche’s planning automation in the Blackbird framework, and Psyche Simulation Reports. All these applications are either new or have been substantially revamped to meet Psyche’s concept of operations. In particular, ensuring the entire toolchain can correctly process epoch-relative activities is discussed. Underlying the main applications are a common set of dependencies developed and maintained by a new cross-mission association of planning developers. In this way, Psyche can inherit well-tested functionality which saves effort and ensures its developers can focus on solving domain challenges. Quality control of the applications and libraries is ensured with a code-review and unit-test based novel ‘CM lite’ process. Collaboration with international industry and academia using the open-source modules is already occurring.The planning and scheduling software is designed to maximize operator awareness of the integrated plan at every step of the process and use common interfaces and file formats to easily transfer information. Design choices plus the team’s test-driven development process enables more expansive capabilities compared to the decentralized planning and sequence generation functions typical of Discovery-class orbiters without significant development cost increases. Benefits and drawbacks of Psyche’s approach are discussed, including comparison to other missions and tools where appropriate.

Ramanathan, Keshav↗

Desert Research and Technology Studies (D-RATS) 2022 Quicklook Report

This report summarizes the Desert Research and Technology Studies (D-RATS) 2022 analog tests. BACKGROUND - Artemis Challenges – NASA’s concept of operations (ConOps) for the Artemis mission architecture brings new challenges for human exploration of the lunar surface, including: (1) Low-angle, natural lighting at lunar poles; and (2) Exploration sites that challenge communication with Earth. - International Partner Involvement – NASA is working with the Japan Aerospace Exploration Agency (JAXA) to scope mission & functional requirements for an Artemis Pressurized Rover (PR), which JAXA may provide. - Charter – HQ Exploration Systems Development Mission Directorate (ESDMD) Moon to Mars Architecture Development Office (M2MADO) Strategy and Architectures (SA) chartered the Human-in-the-Loop (HITL) test team to investigate Artemis architectural questions related to pressurized rover ConOps. - Rationale – to inform the NASA/JAXA pressurized rover study-agreement. PLAN - Objectives – Analog tests conducted in October 2022 by the D-RATS team addressed three high-level objectives: 1. Investigate pressurized rover (PR) ConOps and capabilities for Artemis exploration 2. Integrate with JAXA engineers & astronauts and incorporate JAXA PR design elements into testing. 3. Re-establish analog field-testing skills & capabilities with rovers to investigate Artemis architecture ConOps. - Secondary Objectives – Work with other groups to leverage D-RATS field test for additional objectives. 4. Work with the Public Affairs Office (PAO) to perform D-RATS public outreach activities. 5. Coordinate with the Human Physiology Performance Protection & Operations (H-3PO) team to facilitate in-field evaluation of human health and performance (HHP) objectives. 6. Share D-RATS field-site and assets with Lunar LTE Studies (Lunar LiTES) team, to aid their study of the use of 4G/LTE communication protocols and devices for astronauts and robotic nodes on the lunar surface. - Team – Fully integrated test team comprised of members from 5 NASA centers, JAXA, and the United States Geological Survey (USGS) - Location – Black Point Lava Flow, ~40 miles north of Flagstaff, AZ HIGH-LEVEL OBJECTIVES ACCOMPLISHED - Investigated Pressurized Rover ConOps & Capabilities for Artemis Exploration (Objective 1) - Completed testing with 4 crew pairs, each spending 3 days and 2 nights in the rover conducting Artemis PR dayin-the-life activities (2 JAXA astronauts, 2 JAXA engineers, 1 NASA astronaut, 3 NASA engineers). - Collected detailed objective & subjective data supporting 10 strategic questions related to Artemis PR operations. - Field geologists present in field observed rover operations & EVAs. - Science team in Houston MCC communicated directly with crew. - Demonstrated crew-led and MCC-led PR teleoperation use cases during EVAs. - Integrated with JAXA Engineers & Astronauts and Incorporated JAXA PR Design Elements into Testing (Objective 2) - NASA & JAXA engineers, flight controllers, scientists, roboticists, and astronauts directly participated in and/or observed testing both in field and in MCC-Houston. - Incorporated JAXA PR design elements into both integrated and standalone testing at JSC and in the field. - Re-established Analog Field-Testing Skills & Capabilities with Rovers to Investigate Artemis Architecture ConOps (Objective 3) - Multiple teams successfully worked to establish and manage field-test base camp, monitor and maintain the rover, and plan and execute 2 weeks of consecutive field-testing with little to no breaks between crews. TEST OUTCOMES - Results will inform Artemis architecture ConOps & capabilities related to pressurized rover operations (see sections 2 for more details) - Summary and team detailed reports will be posted on the D-RATS 2022 wiki

Analog↗

Evaluating the Maturity of Cybersecurity Programs for Building Control Systems

The cyber-physical security threat to buildings is complex, non-linear, and rapidly evolving as operational and information technologies converge and connect buildings to cyberspace. Cyberattacks on buildings can exploit smart building controls and breach corporate networks, causing financial and reputational damage. This may result in the loss of sensitive building information or the disruption of, or damage to, the systems necessary for the safe and efficient operation of buildings. For the buildings and facility infrastructure, there is a need for a robust national cybersecurity strategy for buildings, guidance on the selection and implementation of appropriate cybersecurity controls for buildings, an approach to evaluate the maturity and adequacy of the cybersecurity programs. To provide an approach for evaluating the maturity of the cybersecurity programs for building control systems, the US Department of Energy’s widely used Cybersecurity Capability and Maturity Model (C2M2) has been adapted into a building control systems version. The revised model, the Buildings-C2M2 (B-C2M2) provides maturity level indicators for cybersecurity programmatic domains. A “B-C2M2 Lite” version allows facility managers and building control system engineers, or information technology personnel to perform rapid self-assessments of their cybersecurity program. Both tools have been pilot tested on several facilities. This paper outlines the concept of a maturity model, describes the B-C2M2 tools, presents results and observations from the pilot assessments, and lays out plans for future work.

Glantz, Clifford S.↗

3-D simulation codes for EMP E1 formation and propagation

LLNL is developing a suite of modern tools for simulating the generation and propagation of the prompt (E1) electromagnetic pulse (EMP). These include the 3-D EMPulse code, based on particle-in-cell methods and a Cartesian grid in the laboratory frame, and a fast-running companion 3-D code, SHEMP, which employs a vector spherical harmonic decomposition. EMPulse is a comprehensive, first-principles code with full generality and requiring significant computing resources. It tracks Compton electrons directly in the laboratory frame, and includes Ohmic and swarm models for the evolving local conductivity. SHEMP builds on the methods used in Longmire's fast-running CHAP code and in our own CHAP-lite; in those codes, 1-D spherical symmetry is assumed, and the calculation takes advantage of a separation of scales. In SHEMP we seek similar efficiencies in 3-D, incorporating non-spherically-symmetric physics via a pseudospectral approach. We describe these tools and other aspects of our project herein.

45 MILITARY TECHNOLOGY, WEAPONRY, AND NATIONAL DEF↗

Solar System Modeling at NREL

This workshop walked through a description and demonstration of solar modeling data and tools available from the US National Renewable Energy Laboratory (NREL). The topics included: The National Solar Radiation Database (NSRDB) - updates and ways to access this sub-hourly gridded resource data; PVWatts - recent updates to include the NSRDB - opening up much of North and South America for analysis; System Advisor Model (SAM) - SAM is now open source, includes PV + batteries, bifacial panels, and much more; REOpt Lite - an online tool for quick PV and battery sizing for commercial buildings; PV Component LCOE Calculator - Allows for module costing based on components; A detailed demonstration of the SAM model for modeling a PV system with a battery and some of the questions that can be answered.

24 POWER TRANSMISSION AND DISTRIBUTION↗

Behind-the-Meter Solar + Storage Modeling Tool Comparison

Several techno-economic models exist to evaluate solar PV plus battery storage opportunities for behind-the-meter, commercial application. This tool comparison evaluates the methodological differences between several solar plus storage modeling tools and employs a case study to compare their outputs. The tools selected for comparison include NREL's REopt Lite, NREL's System Advisor Model (SAM), Homer Energy's Homer Grid, Geli ESyst, EPRI StorageVET, and LBL's DER-CAM. The comparison focuses on each tool's simulation and optimization capabilities, PV and battery performance modeling, dispatch strategies, and economic model assumptions and calculations, with brief mention of additional capabilities outside the scope of behind-the-meter PV and battery storage. This report describes the case study's tool inputs and outputs and dives into the differences in assumptions, methodologies, and capabilities between tools.

29 ENERGY PLANNING, POLICY, AND ECONOMY↗

City Decision Analysis Resources and Tools

The City Decision Analysis at Any Scale Workshop was held February 17-18, 2021. This brochure was created to provide participants with a collection of tools and resources that support planning and implementation of clean energy goals for communities and businesses.

ACES↗

Structures and Mechanisms of a Novel Bacterial Transport System for Fatty Acids

Abstract Bacterial acquisition of metabolites is largely facilitated by transporters with unique substrate scopes. The tripartite ATP‐independent periplasmic (TRAP) transporters comprise a large family of bacterial proteins that facilitate the uptake of a variety of small molecules. It has been reported that some TRAP systems encode a fourth protein, the T component. The T‐component, or TatT, is predicted to be a periplasmic‐facing lipoprotein that enables the uptake of metabolites from the outer membrane. However, no substrates were revealed for any TatT and their functional role(s) remained enigmatic. We recently identified a homolog in Methylococcus capsulatus that binds to sterols, and herein, we report two additional homologs that demonstrate a preference for long‐chain fatty acids. Our bioinformatics, quantitative analyses of protein‐ligand interactions, and high‐resolution crystal structures suggest that TatTs might facilitate the trafficking of hydrophobic or lipophilic substrates and represent a new class of bacterial lipid and fatty acid transporters.

59 BASIC BIOLOGICAL SCIENCES↗

Effects of naturally occurring charged mutations on the structure, stability, and binding of the Pin1 WW domain

Pin1 is a peptidyl-prolyl cis-trans isomerase, whose WW domain specifically recognizes the pSer/Thr-Pro motif. Pin1 is involved in multiple phosphorylation events that regulate the activities of various substrates, and Pin1 deregulation has been reported in various diseases, including cancer and Alzheimer's disease. The WW domain of Pin1 has been used as a small model protein to investigate the folding mechanisms of the β-sheet structure by studying the effect of mutations or its naturally occurring variants. However, only a few studies have investigated the structure and binding of Pin1 WW mutants. In the present work, two naturally occurring Pin1 WW variants, namely, G20D and S16R, derived from the cynomolgus monkey and African green monkey, respectively, were selected to investigate the influence of charge mutation on the structure, stability, and binding properties of the Pin1 WW domain. Analysis using a combination of nuclear magnetic resonance (NMR) and chemical shift-based calculations revealed that the G20D and S16R mutants had high structural similarity to the wild-type Pin1 WW domain. However, the presence of a charge mutation significantly decreased the stability of the Pin1 WW domain. Both the wild-type and G20D forms of the Pin1 WW domain utilized a three-site mode to bind to a phosphorylated Tau peptide, pT231, whereas the S16R mutant binds to the pT231 peptide either in a non-specific manner or through a totally different binding mechanism. Correspondingly, the wild-type and two mutant Pin1 WW domains showed different binding affinities to the Tau phosphopeptide. Considering that the WW domain participates in the catalytic activity of the Pin1 isomerase, our study represents a novel approach for studying Pin1 function through the analysis of its naturally occurring mutants. - Highlights: • G20D and S16R Pin1 WW had high structure similarity to wild-type. • Stabilities of G20D and S16R mutant Pin1 WW significantly decreased. • wild-type and G20D Pin1 WW utilized three-site mode for phosphorylated Tau binding. • wild-type and mutant Pin1 WW showed differential binding affinities to substrate.

60 APPLIED LIFE SCIENCES↗

Growth, structure, defects and polarized absorption spectral properties of Er:Yb:YCa{sub 4}O(BO{sub 3}){sub 3} crystals

YCa{sub 4}O(BO{sub 3}){sub 3} (YCOB) crystals co-doped with 3 at% Er{sup 3+} and 20 at% Yb{sup 3+} were successfully grown by the Czochralski method. X-ray powder diffraction (XRPD) results show that the as-grown Er:Yb:YCOB crystal belongs to the monoclinic system with space group Cm. And the lattice parameters are a=8.076(8) Å, b=16.023(7) Å, c=3.528(4) Å and β=101.15(4)°. Crystal defects were revealed by chemical etching experiments. The density of etch pits, attributed to dislocations and observed on (010) planes, was found to be not uniform along the crystal diameter. The detailed polarized absorption spectra were measured. The polarized absorption cross sections at 977 nm are 1.01×10{sup −20}, 1.22×10{sup −20} and 1.05×10{sup −20} cm{sup 2} for E//X, E//Y and E//Z, respectively. And the polarized absorption cross sections at 1538 nm is about 1.86×10{sup −20} cm{sup 2} for both E//X and E//Z, but for E//Y the result is 1.03×10{sup −20} cm{sup 2}, which is much smaller. The relationship between the crystal structure and absorption spectra was discussed. - Graphical abstract: The as-grown bulk Er:Yb:YCOB crystal (the long axis is along the b-axis) and LeBail fit to the XRPD pattern of Er:Yb:YCOB (top) compared to the diffraction pattern of YCOB from the JCPDS data bank (bottom). Reflection markers indicate the calculated reflection positions. The middle trace gives the difference between observed and calculated intensity. Reflection indices corresponding to the strongest diffraction peaks are marked on the experimental pattern. - Highlights: • Er:Yb:YCOB crystal with a new composition was grown. • XRPD and FT-IR showed a certain degree of deformation in the crystal structure. • Detailed polarized absorption cross sections at 977 nm and 1538 nm were calculated. • Crystal defects were clearly revealed by chemical etching experiments.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

Deubiquitinase USP33 is negatively regulated by β-TrCP through ubiquitin-dependent proteolysis

Ubiquitin-mediated proteolysis regulates cellular levels of various proteins, and therefore plays important roles in controlling cell signaling and disease progression. The Skp1-Cul1-F-box ubiquitin ligase β-TrCP is recognized as an important negative regulator for numerous key signaling proteins. Recently, the deubiquitinases (DUBs) have turned out to be essential to regulate signaling pathways related to human diseases. However, whether β-TrCP is able to regulate the deubiquitinase family members remains largely unexplored. Here, we found that β-TrCP downregulated cellular levels of endogenous USP33. We also revealed that β-TrCP interacted with USP33 independently of the classic binding motif for β-TrCP, and mediated USP33 degradation via the ubiquitin proteasome pathway. Furthermore, we found that the WD40 motif of β-TrCP and 201–400 amino acid motif of USP33 are required for the interaction between β-TrCP and USP33. Consequently, β-TrCP attenuated USP33-mediated inhibition of cell proliferation and cell invasion. Taken together, our study clarified that the E3 ligase β-TrCP regulates cellular USP33 levels by the ubiquitin-proteasomal proteolysis. - Highlights: •β-TrCP downregulates protein levels of USP33. •β-TrCP promotes USP33 ubiquitination and degradation. • The effect of β-TrCP on USP33 is independent of DSG motif. • β-TrCP attenuates USP33-mediated suppression of cell proliferation and invasion.

60 APPLIED LIFE SCIENCES↗

Elucidating Phosphate and Cadmium Cosorption Mechanisms on Mineral Surfaces with Direct Spectroscopic and Modeling Evidence

The simultaneous sorption of cations and anions at the mineral–water interface can substantially alter their individual sorption characteristics; however, this phenomenon lacks a mechanistic understanding. Our study provides direct spectroscopic and modeling evidence of the molecular cosorption mechanisms of the cadmium ion (Cd 2+ ) and phosphate (P) on goethite and layered manganese (Mn) oxide of birnessite, through in situ attenuated total reflection Fourier-transform infrared (ATR-FTIR), P K-edge X-ray absorption near-edge structure (XANES) spectroscopy, and surface complexation modeling. Phosphate synergistically cosorbed with Cd on goethite predominantly through P-bridged ternary complexes (≡Fe–P–Cd) and electrostatic interactions at wide pH conditions. Likewise, P and Cd exhibited synergistic cosorption on birnessite by forming P-bridged ternary complexes (≡Mn–P–Cd) and weak competitive sorption at the layer edge sites. As pH and Cd loading increased, the surface P species transitioned from a binary complex to a ternary complex and/or Cd 3 (PO 4 ) 2 precipitate for both goethite and birnessite. Compared to that in solution at pH 8, the formation of Cd 3 (PO 4 ) 2 was inhibited by the presence of goethite and birnessite, ascribed to the specific adsorption of P and Cd, more pronounced in birnessite due to the stronger sorption of Cd at its vacant sites. Finally, the discovered cosorption mechanisms of P and Cd have important implications for understanding and predicting their mobility and availability in Cd-contaminated settings.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗