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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 271 records · Page 15
All sourcesNASA NTRSDOE OSTI
DOE OSTI2013

Flavivirus infection from mosquitoes in vitro reveals cell entry at the plasma membrane

Dengue and West Nile viruses are enveloped RNA viruses that belong to genus Flavivirus (family Flaviviridae) and are considered important mosquito-borne viral pathogenic agents worldwide. A potential target for intervention strategies is the virus cell entry mechanism. Previous studies of flavivirus entry have focused on the effects of biochemical and molecular inhibitors on viral entry leading to controversial conclusions suggesting that the process is dependent upon endocytosis and low pH mediated membrane fusion. In this study we analyzed the early events in the infection process by means of electron microscopy and immuno-gold labeling of viral particles during cell entry, and used as a new approach for infecting cells with viruses obtained directly from mosquitoes. The results show that Dengue and West Nile viruses may infect cells by a mechanism that involves direct penetration of the host cell plasma membrane as proposed for alphaviruses.

60 APPLIED LIFE SCIENCES↗
DOE OSTI2014

The V domain of dog PVRL4 (nectin-4) mediates canine distemper virus entry and virus cell-to-cell spread

The entry of canine distemper virus (CDV) is a multistep process that involves the attachment of CDV hemagglutinin (H) to its cellular receptor, followed by fusion between virus and cell membranes. Our laboratory recently identified PVRL4 (nectin-4) to be the epithelial receptor for measles and canine distemper viruses. In this study, we demonstrate that the V domain of PVRL4 is critical for CDV entry and virus cell-to-cell spread. Furthermore, four key amino acid residues within the V domain of dog PVRL4 and two within the CDV hemagglutinin were shown to be essential for receptor-mediated virus entry. - Highlights: • PVRL4 (nectin-4) is the epithelial cell receptor for measles and canine distemper viruses. • V domain of PVRL4 is critical for CDV entry, cell-to-cell spread, and syncytia formation. • Chimeric PVRL1 backbone substituted with the V domain of PVRL4 can function as a receptor. • Amino acids (F132/P133/A134/G135) within the V domain are essential for PVRL4 receptor activity. • Amino acids (P493/Y539) within CDV H protein are essential for PVRL4 receptor interaction.

60 APPLIED LIFE SCIENCES↗
DOE OSTI2014

Molecular determinants of dengue virus 2 envelope protein important for virus entry in FcγRIIA-mediated antibody-dependent enhancement of infection

Antibody-dependent enhancement (ADE) of infection may cause severe illness in patients suffering a secondary infection by a heterologous dengue virus (DENV) serotype. During ADE of infection, cross-reactive non- or poorly-neutralizing antibodies form infectious virus-Ab complexes with the newly infecting serotype and enhance virus infection by binding to the Fcγ receptors (FcγR) on FcγR-bearing cells. In this study, we determined that molecular determinants of DENV2 envelope protein critical for virus entry during non-ADE infection are also required for ADE infection mediated by FcγRIIA, and binding of virus-Ab complexes with FcγRIIA alone is not sufficient for ADE of infection. The FcγRIIA mainly plays an auxiliary role in concentrating the virus–Ab complex to the cell surface, and other primary cellular receptors are required for virus entry. Understanding the viral entry pathway in ADE of DENV infection will greatly facilitate rational designs of anti-viral therapeutics against severe dengue disease associated with ADE. - Highlights: • KKK305/307/310 in DENV2 E-DIII is critical for virus attachment in ADE and non-ADE infection. • Binding of DENV2–Ab complex with FcγRII alone is not sufficient for virus entry in ADE infection. • Other primary receptors were required for DENV2 internalization during FcγRII–mediated ADE. • G104 and L135 of DENV2 E are critical for virus-mediated membrane fusion. • DENV2 virus-mediated membrane fusion is required for both ADE and non-ADE infection.

60 APPLIED LIFE SCIENCES↗
DOE OSTI2014

Identification of residues on human receptor DPP4 critical for MERS-CoV binding and entry

Middle East respiratory syndrome coronavirus (MERS-CoV) infects host cells through binding the receptor binding domain (RBD) on its spike glycoprotein to human receptor dipeptidyl peptidase 4 (hDPP4). Here, we report identification of critical residues on hDPP4 for RBD binding and virus entry through analysis of a panel of hDPP4 mutants. Based on the RBD–hDPP4 crystal structure we reported, the mutated residues were located at the interface between RBD and hDPP4, which potentially changed the polarity, hydrophobic or hydrophilic properties of hDPP4, thereby interfering or disrupting their interaction with RBD. Using surface plasmon resonance (SPR) binding analysis and pseudovirus infection assay, we showed that several residues in hDPP4–RBD binding interface were important on hDPP4–RBD binding and viral entry. These results provide atomic insights into the features of interactions between hDPP4 and MERS-CoV RBD, and also provide potential explanation for cellular and species tropism of MERS-CoV infection. - Highlights: • It has been demonstrated that MERS-CoV infects host cells through binding its envelope spike (S) glycoprotein to the host cellular receptor dipeptidyl peptidase 4 (DPP4). • To identify the critical residues on hDPP4 for RBD binding and virus entry, we constructed a panel of hDPP4 mutants based on structure-guided mutagenesis. • Using surface plasmon resonance (SPR) binding analysis and pseudovirus infection assay, we showed that several residues on hDPP4 had significant impacts on virus/receptor interactions and viral entry. • Our study has provided new insights into the features of interactions between hDPP4 and MERS-CoV RBD, and provides potential explanation for cellular and species tropism of MERS-CoV infection.

60 APPLIED LIFE SCIENCES↗
DOE OSTI2015

Contribution of N-linked glycans on HSV-2 gB to cell–cell fusion and viral entry

HSV-2 is the major cause of genital herpes and its infection increases the risk of HIV-1 acquisition and transmission. HSV-2 glycoprotein B together with glycoproteins D, H and L are indispensable for viral entry, of which gB, as a class III fusogen, plays an essential role. HSV-2 gB has seven potential N-linked glycosylation (N-CHO) sites, but their significance has yet to be determined. For the first time, we systematically analyzed the contributions of N-linked glycans on gB to cell–cell fusion and viral entry. Our results demonstrated that, of the seven potential N-CHO sites on gB, mutation at N390, N483 or N668 decreased cell–cell fusion and viral entry, while mutation at N133 mainly affected protein expression and the production of infectious virus particles by blocking the transport of gB from the endoplasmic reticulum to Golgi. Our findings highlight the significance of N-linked glycans on HSV-2 gB expression and function. - Highlights: • N-linked glycan at N133 is important for gB intracellular trafficking and maturation. • N-linked glycans at N390, N483 and N668 on gB are necessary for optimal cell–cell fusion. • N-linked glycans at N390, N483 and N668 on gB are necessary for optimal viral entry.

60 APPLIED LIFE SCIENCES↗
DOE OSTI2013

Experimental energy resolution of a paracentric hemispherical deflector analyzer for different entry positions and bias

A specially designed hemispherical deflector analyzer (HDA) with 5-element input lens having a movable entry position R{sub 0} suitable for electron energy analysis in atomic collisions was constructed and tested. The energy resolution of the HDA was experimentally determined for three different entry positions R{sub 0}= 84, 100, 112 mm as a function of the nominal entry potential V(R{sub 0}) under pre-retardation conditions. The resolution for the (conventional) entry at the mean radius R{sub 0}= 100 mm was found to be a factor of 1.6-2 times worse than the resolution for the two (paracentric) positions R{sub 0}= 84 and 112 mm at particular values of V(R{sub 0}). These results provide the first experimental verification and a proof of principle of the utility of such a paracentric HDA, while demonstrating its advantages over the conventional HDA: greater dispersion with reduced angular aberrations resulting in better energy resolution without the use of any additional fringing field correction electrodes. Supporting simulations of the entire lens plus HDA spectrometer are also provided and mostly found to be within 20%-30% of experimental values. The paracentric HDA is expected to provide a lower cost and/or more compact alternative to the conventional HDA particularly useful in modern applications utilizing a position sensitive detector.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗
DOE OSTI2015

Stock-Specific Size and Timing at Ocean Entry of Columbia River Juvenile Chinook Salmon and Steelhead: Implications for Early Ocean Growth

Abstract Juvenile salmon transitioning from freshwater to marine environments experience high variation in growth and survival, yet the specific causes of this variation are poorly understood. Size at and timing of ocean entry may contribute to this variation because they influence both the availability of prey and vulnerability to predators. To explore this issue, we used stock assignments based on genetic stock identification and internal tags to document the stock-specific size and timing of juvenile hatchery and presumed wild Columbia River Chinook Salmon Oncorhynchus tshawytscha and steelhead O. mykiss at ocean entry during 2007–2011. We found that juvenile salmon and steelhead had consistent stock-specific capture dates, with lower-river stocks typically having earlier timing than those originating farther upstream. Mean size also varied among stocks and was related to hatchery practices. Hatchery yearling Chinook Salmon and steelhead were consistently larger than wild fish from the same stocks, although timing in the estuary was similar. In contrast, hatchery subyearling Chinook Salmon were of similar size to wild fish but entered the ocean up to a month earlier. We evaluated the potential importance of these traits on early marine growth by estimating stock-specific growth rates for Chinook Salmon caught in estuarine and ocean habitats. Growth rates were related to relative ocean entry timing, with lower growth rates for stocks that had only recently arrived in marine waters. Our results demonstrate that stocks within a single basin can differ in their size and timing of ocean entry, life history traits that contribute to early marine growth and potentially to the survival of juvenile salmon. Our results also highlight the necessity of considering stock-specific variation in life history traits to understand salmon ecology and survival across the entire life cycle. Received January 16, 2015; accepted April 28, 2015

Weitkamp, Laurie A.↗
DOE OSTI2013

Structural and functional studies of a phosphatidic acid-binding antifungal plant defensin MtDef4: Identification of an RGFRRR motif governing fungal cell entry

A highly conserved plant defensin MtDef4 potently inhibits the growth of a filamentous fungus Fusarium graminearum. MtDef4 is internalized by cells of F. graminearum. To determine its mechanism of fungal cell entry and antifungal action, NMR solution structure of MtDef4 has been determined. The analysis of its structure has revealed a positively charged patch on the surface of the protein consisting of arginine residues in its γ-core signature, a major determinant of the antifungal activity of MtDef4. Here, we report functional analysis of the RGFRRR motif of the γ-core signature of MtDef4. The replacement of RGFRRR to AAAARR or to RGFRAA not only abolishes fungal cell entry but also results in loss of the antifungal activity of MtDef4. MtDef4 binds strongly to phosphatidic acid (PA), a precursor for the biosynthesis of membrane phospholipids and a signaling lipid known to recruit cytosolic proteins to membranes. Mutations of RGFRRR which abolish fungal cell entry of MtDef4 also impair its binding to PA. Our results suggest that RGFRRR motif is a translocation signal for entry of MtDef4 into fungal cells and that this positively charged motif likely mediates interaction of this defensin with PA as part of its antifungal action.

antifungal agent↗
DOE OSTI2025

Acoustic Observations of the OSIRIS-REx Sample Return Capsule Re-Entry from Wendover Airport

The Origins, Spectral Interpretation, Resource Identification, and Security‐Regolith Explorer sample return capsule (SRC) re‐entered the Earth’s atmosphere at hypersonic speeds from interplanetary space on 24 September 2023. The current work reports on 18 ground‐based acoustic sensors deployed at Wendover Airport, the same location that the Genesis and Stardust SRC re‐entries were recorded. Four different sensors (Chaparral Physics, Gem, Wilson Engineering Research and Development [WERD], and RedVox) were deployed in close proximity to compare their performance. All the sensors captured an N‐wave signal associated with the SRC re‐entry shock wave followed by a broadband coda. The Chaparral Physics array served as the high‐fidelity reference measurement. The N‐wave signal had a peak‐to‐peak amplitude of 4.07 Pa with a fundamental frequency of 4.98 Hz from 167.5° measured clockwise from north, nearly perpendicular to the SRC trajectory. In addition, high coherence in the coda was shown to be associated reflections from the surrounding mountains. In general, the more economical sensors (Gem, WERD, and RedVox) produced results that were consistent with these observations and sensor specifications. Beamforming with these single sensors arranged as an array showed agreement with the high‐fidelity array to within a couple of degrees. Furthermore, the current high‐fidelity results were compared with the measurements during the Genesis and Stardust SRC re‐entries. All three entries produced a broadband fundamental peak at a frequency that was inversely related to the SRC diameter as well as evidence of reflections from the surrounding topography.

KC, Real J. [Oklahoma State University, Stillwater↗
DOE OSTI2021

Entry and Disposition of Zika Virus Immune Complexes in a Tissue Culture Model of the Maternal-Fetal Interface

Zika virus (ZIKV) infections have been associated with an increased incidence of severe microcephaly and other neurodevelopmental disorders in newborn babies. Passive immunization with anti-ZIKV neutralizing antibodies has the potential to become a feasible treatment or prophylaxis option during pregnancy. Prior to clinical use, such antibodies should be assessed for their ability to block ZIKV passage to the fetus. We used human placental and mammalian cell monolayers that express FcRn and laboratory preparations of anti-ZIKV antibodies as a model system to investigate the disposition of ZIKV/antibody immune complexes (ICs) at the maternal-fetal interface. We further characterized solution properties of the ICs to evaluate whether these are related to in vitro effects. We found that both ZIKV and ZIKV envelope glycoprotein can enter and passage through epithelial cells, especially those that overexpress FcRn. In the presence of ZIKV antibodies, Zika virus entry was bimodal, with reduced entry at the lowest (0.3–3 ng/mL) and highest (µg/mL) antibody concentrations. Intermediate concentrations attenuated inhibition or enhanced viral entry. With respect to anti-ZIKV antibodies, we found that their degradation was accelerated when presented as ICs containing increased amounts of ZIKV immunogen. Of the two monoclonal antibodies tested, the preparation with higher aggregation also exhibited higher degradation. Our studies confirm that intact Zika virus and its envelope immunogen have the potential to enter and be transferred across placental and other epithelial cells that express FcRn. Presence of anti-ZIKV IgG antibodies can either block or enhance cellular entry, with the antibody concentration playing a complex role in this process. Physicochemical properties of IgG antibodies can influence their degradation in vitro.

60 APPLIED LIFE SCIENCES↗
NASA NTRS1972

Apollo experience report: Mission planning for Apollo entry

The problems encountered and the experience gained in the entry mission plans, flight software, trajectory-monitoring procedures, and backup trajectory-control techniques of the Apollo Program should provide a foundation upon which future spacecraft programs can be developed. Descriptions of these entry activities are presented. Also, to provide additional background information needed for discussion of the Apollo entry experience, descriptions of the entry targeting for the Apollo 11 mission and the postflight analysis of the Apollo 10 mission are presented.

Graves, C. A.↗
NASA NTRS1972

Separation and communications geometry analysis for a Jupiter entry probe from a Pioneer spacecraft

The separation and communications geometry analysis is presented which shows that it is possible to target a Jupiter entry probe from a Pioneer spin-stabilized vehicle to desired entry conditions and maintain a continuous line-of-sight communications link while the probe descends to a pressure of 10 bars within the atmosphere. Such a targeting is possible with either the deflected probe mode or with the deflected bus mode. For the nominal mission studied, an 800 day trip in 1978 with the Pioneer type spacecraft flying by a 1.3 R(J), it was possible to achieve entries near zero angle of attack at about -25 deg relative entry flight path angle. Because of the constraint on the deflection maneuver for the deflected probe mode, the deflection and phasing velocity requirements are much larger for this mode. The geometry parameters, look angles, ranges and range rates, provided by this analysis permit the design of a functional communications system for the bus and probe. Avoiding a high trapped radiation belt hazard at Jupiter by flying the bus by at 6 R(J), however, requires using a despun antenna on the flyby bus.

Tindle, E. L.↗
NASA NTRS1973

Structure of the ionospheric disturbances about planetary entry probes

Local ionospheric disturbances which would be created by a planetary entry probe are investigated. Competing theories of spacecraft-ambient plasma interactions are used to estimate computationally the perturbations of the plasma, particularly the structure of the near wake behind planetary entry vehicles. The results have bearing on the location and operation of plasma diagnostic instrumentation aboard planetary entry vehicles. Recent estimates of Mars ionospheric properties plus vehicle dimensions and speeds similar to those of the Viking Mars Lander are used to define the parameters essential to the theory. Smaller entry bodies are also considered. Comparisons are made of the results based on the different theories for a given assumed planetary atmosphere, and also with the perturbations a similar vehicle would generate in the earth's ionosphere.

Weil, H.↗
NASA NTRS1974

Effect of initial conditions on deduced atmosphere for Uranus and Jupiter entries

Atmosphere reconstruction based on the data from a probe is discussed in terms of the effect of errors in entry velocity and entry flight path angle on the determination of density, pressure, and temperature as functions of altitude. Emphasis is placed on Uranus and Jupiter entries, although Saturn entries are included.

Kirk, D.↗
NASA NTRS1974

Planetary entry aerothermodynamics. II - Computational analyses and flight experience

Limitations concerning the possibility to simulate all the significant flow and thermal phenomena occurring during the entry of a space vehicle into a planetary atmosphere make it necessary to rely on computational analyses to obtain the required data for the design of the spacecraft needed for the NASA missions planned for the next two decades. 'Benchmark' computer programs concerned with complete, detailed, and accurate computational solutions of entry problems are considered along with programs representing engineering approximations for cases in which the accuracy provided by the benchmark programs is not needed. The information obtainable by computational analysis has to be supplemented by actual flight experience in order to meet the goals of the NASA entry-technology program. The individual space missions planned for the coming years are examined together with the possibilities for obtaining the data needed to satisfy the entry requirements in each case.

Olstad, W. B.↗
NASA NTRS1976

Planetary/DOD entry technology flight experiments. Volume 1: Executive summary

The feasibility of using the space shuttle to launch planetary and DoD entry flight experiments was examined. The results of the program are presented in two parts: (1) simulating outer planet environments during an earth entry test, the prediction of Jovian and earth radiative heating dominated environments, mission strategy, booster performance and entry vehicle design, and (2) the DoD entry test needs for the 1980's, the use of the space shuttle to meet these DoD test needs, modifications of test procedures as pertaining to the space shuttle, modifications to the space shuttle to accommodate DoD test missions and the unique capabilities of the space shuttle. The major findings of this program are summarized.

Christensen, H. E.↗
NASA NTRS1976

Drag bias feedback for the analytic drag control entry guidance system

The Analytic Drag Control (ADC) entry guidance has been developed and baselined for the space shuttle orbiter entry. A method is presented which corrects the orbiter entry guidance commanded bank angle for biases between navigated drag and guidance computed reference drag. This is accomplished by an integral feedback technique, which uses the drag bias information to adjust the difference between navigated and reference altitude rate used by the ADC guidance. The method improves the capability of the ADC guidance system by compensating for any error source which causes a bias between the navigated drag and reference drag profile. These errors include navigated altitude rate errors, atmosphere dispersions, and roll attitude deadband effects. A discussion of the method and results of digital computer entry simulations is presented.

Kyle, H. C.↗
NASA NTRS1977

Electrical power requirements analysis. Single failure tolerant entry

The results of an analysis of the orbiter electrical power system for the case of a single failure tolerant (SFT) entry are presented. The analysis was performed using the shuttle electrical power system analysis computer program. It was performed to permit assessment of the capability of the orbiter systems to support the proposed entry configuration and to provide the data necessary to identify potential constraints and limitations. Three contingency modes have been identified which would require an SFT entry. This analysis addresses an SFT entry resulting from the loss of two fuel cell powerplants, while on orbit. The results of the analysis indicate that, even under near optimum conditions, the fuel cell power demand will exceed the tested operating capacity of 16 kw, and that various electrical components may experience voltages below 24 VDC.

Pipher, M. D.↗