Microbial Bioburden on NASA Cleanroom Surfaces: Cell Counting and Fluorescence in situ Hybridization
Spacecraft assembly cleanrooms can harbor a low number of microbial cells that could contaminate hardware designed for life detection missions (known as “bioburden”). We are evaluating epifluorescence microscopy and fluorescence in situ hybridization (FISH) as rapid, accurate, and cost-effective methods to complement other contamination detection techniques. This study is part of a larger effort to characterize background organic contamination and the efficacy of cleaning procedures in Johnson Space Center (JSC) Astromaterials Curation Laboratories and Goddard Space Flight Center (GSFC) spacecraft assembly rooms. The low level of biomass required careful use of blanks during each stage of sample collection and preparation. FISH was performed using general archaeal and bacterial probes, as well as some group-specific probes. While some active bacteria were identified, a large majority of them were dead or inactive, and thus were difficult to identify by standard FISH. Our results are consistent with other studies that show that the bioburden present in clean rooms includes active, dormant, and dead cells.