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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 271 records · Page 15
All sourcesNASA NTRSDOE OSTI
NASA NTRS2013

Horizontal Habitability Layout Studies

As our efforts in the 2011-2012 X-Hab Academic Innovation Challenge we designed, developed, constructed and tested a deployable horizontally oriented habitat system for use as an Earth analog. The goals of the project included a short-term goal of an interdisciplinary senior design project to design, build and evaluate a horizontally oriented habitat and a long-term goal to develop capabilities in education, research, and outreach in field of space habitat design. This included both technical engineering and outreach efforts. In the 2011-2012 year, the program involved the following schools at OSU: The Schools of Mechanical and Aerospace Engineering (MAE) and Industrial and Engineering Management (IEM) within the College of Engineering, Architecture and Technology and Design, Housing and Merchandising (DHM) in the College of Human Sciences. We also included the School of Aviation Education (AvED) in the College of Education through the ISNPIRE (Interdisciplinary National Science Project Incorporating Research and Education Experience) program of as part of our outreach efforts.

Jamey D. Jacob↗
DOE OSTI2020

Method and precision nanopositioning apparatus with compact vertical and horizontal linear nanopositioning flexure stages for implementing enhanced nanopositioning performance

An enhanced method and precision nanopositioning apparatus for implementing enhanced nanopositioning performance is provided. The nanopositioning apparatus includes a vertical linear nanopositioning flexure stage and a horizontal linear nanopositioning flexure stage. The vertical linear nanopositioning flexure stage includes a stage base, a symmetrically configured flexure linear guiding mechanism with precision motion enhancement structure, and a stage carriage. The horizontal linear nanopositioning flexure stage is mounted on the stage carriage of the vertical linear nanopositioning flexure stage, and includes a middle-bar relative position control mechanism to enhance the stiffness of a flexure linear guiding mechanism.

77 NANOSCIENCE AND NANOTECHNOLOGY↗
DOE OSTI2021

Engineering organisms resistant to viruses and horizontally transferred genetic elements

Organisms resistant to horizontal gene transfer (HGT), and compositions and methods of use thereof are provided. The organisms are typically genomically recoded organisms (GRO), typically cells, having a genome in which at least one endogenous codon has been eliminated by reassignment of the codon to a synonymous or non-synonymous codon. The GRO typically include a recombinant expression construct for expression of at least one element of a ribosomal rescue pathway, typically lacking the eliminated codon. Typically, the disclosed cells are resistant to completed transfer and/or expression of a horizontally transferred genetic element (HTGE) from another organism compared a corresponding cell having a genome wherein the eliminated codon has not been eliminated. In some embodiments, the organism from which the HTGE is being transferred is a bacterium or a virus.

Isaacs, Farren J.↗
DOE OSTI2020

Reduction of the Waste Occupied Area by Nuclide Separation and Horizontal Emplacement of the Waste Packages - 20422

Two of the key objectives for realizing the advanced nuclear fuel cycle are to reduce the volume of high-level radioactive waste and to reduce the foot-print of the geological repository. To achieve these objectives, research and development on nuclide separation technologies is a prerequisite. In this context, it is necessary to consider the vitrification conditions and the design of the geological repository where separation technologies would be implemented to achieve effective reductions in the volume of vitrified waste and the foot-print of the geological repository. In this study, the effects of the vitrification conditions on the number of vitrified waste units and the disposal area were evaluated, based on the assumption that separation of Cs and Sr from the spent nuclear fuel had been performed. In addition, with reference to the design of the geological repository, the impact on the foot-print of the repository for different emplacement methods of the waste packages was examined. As a result, it was clarified that the waste-occupied area could be reduced by horizontal emplacement of the vitrified waste with low heat generation. In addition, it was demonstrated that a low waste-loaded vitrified waste contributed to a reduction in the foot-print of the geological repository in the case of horizontal emplacement, although the number of vitrified waste units increased. (authors)

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗
DOE OSTI2020

Sensitivity and history match analysis of a carbon dioxide “huff-and-puff” injection test in a horizontal shale gas well in Tennessee

Due to improvements in well development, shale gas production has gained importance recently, especially in the United States. To improve gas production and develop a better understanding of shale reservoirs, researchers are conducting field tests to monitor CO 2 storage and enhanced gas recovery. Reservoir simulations can then utilize these field results for sensitivity studies, uncertainty analysis, history matching, gas production forecasts, and CO 2 storage capacity estimations. One such field test was performed for the Chattanooga Shale formation in Morgan County, Tennessee. Approximately 463 tonnes (510 tons) of CO 2 was successfully injected into a hydraulically fractured horizontal well over a thirteen-day period in March 2014. The injection test was achieved in four steps: pre-injection, injection, soaking, and flowback. In this paper, those steps were modeled with a reservoir simulator to match the historic production and injection rates with gas component compositions and forecast the production for five years. The reservoir fluid was modeled as a multi-component gas, including CH 4 , C 2 H 6 , C 3 H 8 , N 2 , and CO 2 . Langmuir constants, reservoir pressure, and fracture network volume were adjusted to match simulation results with observed production rates and gas composition. Results showed that, under the same reservoir conditions, each gas component behaves differently by way of compositional changes in production. CH4 behaves like N2, while CO 2 behaves like heavier hydrocarbons such as C 2 H 6 and C 3 H 8 . CO 2 plume results showed that, after injection, the produced CO 2 is mostly derived from fractured limestone (i.e., Fort Payne Formation) because the injected CO 2 is not adsorbed in limestone but rather by shale formations.

03 NATURAL GAS↗
DOE OSTI2020

On the spectral behaviour of the turbulence-driven power fluctuations of horizontal-axis turbines

In this article we consider the spectral behaviour of turbulence-driven power fluctuations for a single horizontal-axis turbine. To this end, a small-scale instrumented axial-flow hydrokinetic turbine model ( diameter=0.724 m ) is deployed in the long water flume situated in the laboratory facilities of IFREMER in Boulogne-sur-Mer, France, and synchronous measurements of the upstream velocity and the rotor are collected for different tip-speed ratios. The study confirms previous findings suggesting that the power spectra follow the velocity spectra behaviour in the large scales region and a steeper power law slope behaviour ( -11/3 ) over the inertial frequency sub-range. However, we show that both the amplitude of the power spectra and low-pass filtering effect over the inertial sub-range also depend on the rotor aero/hydrodynamics (e.g. dC L /dα) and the approaching flow deceleration and not solely on the rotational effects. In addition, we present a novel semi-analytical model to predict the dominant blade-passing frequency harmonics in the high-frequency regime using the rotationally sampled spectra technique. For all calculations, the distortion of incoming turbulence is taken into account.

42 ENGINEERING↗
DOE OSTI2024

Comparison of horizontal wind speed and direction measurements from dual-Doppler radar and profiling lidars

Dual-Doppler radar is a relatively new technology in the wind energy community and thus not yet studied vastly. This paper aims to compare horizontal wind speed and direction data retrieved from dual-Doppler radar and profiling lidars within the American WAKE experimeNt (AWAKEN) to investigate the influence of measurement height, wind direction and speed on the comparison. The 10-min averaged data show a better agreement of the measurements for higher altitudes, especially at faster wind speeds. For the wind direction, two sectors of larger differences in the measurements were detected: around 270° transient winds occur with a higher frequency than in other sectors. To explain the different measurement values in the wind direction sector around 90°, further studies, e.g. on the influence of atmospheric stability, are necessary.

17 WIND ENERGY↗
DOE OSTI2024

Toxin-Antitoxin Systems Reflect Community Interactions Through Horizontal Gene Transfer

Bacterial evolution through horizontal gene transfer (HGT) reflects their community interactions. In this way, HGT networks do well at mapping community interactions, but offer little toward controlling them—an important step in the translation of synthetic strains into natural contexts. Toxin–antitoxin (TA) systems serve as ubiquitous and diverse agents of selection; however, their utility is limited by their erratic distribution in hosts. Here we examine the heterogeneous distribution of TAs as a consequence of their mobility. By systematically mapping TA systems across a 10,000 plasmid network, we find HGT communities have unique and predictable TA signatures. We propose these TA signatures arise from plasmid competition and have further potential to signal the degree to which plasmids, hosts, and phage interact. To emphasize these relationships, we construct an HGT network based solely on TA similarity, framing specific selection markers in the broader context of bacterial communities. This work both clarifies the evolution of TA systems and unlocks a common framework for manipulating community interactions through TA compatibility.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2022

Dead but Not Forgotten: How Extracellular DNA, Moisture, and Space Modulate the Horizontal Transfer of Extracellular Antibiotic Resistance Genes in Soil

Antibiotic-resistant bacteria and the spread of antibiotic resistance genes (ARGs) pose a serious risk to human and veterinary health. While many studies focus on the movement of live antibiotic-resistant bacteria to the environment, it is unclear whether extracellular ARGs (eARGs) from dead cells can transfer to live bacteria to facilitate the evolution of antibiotic resistance in nature. Here, we use eARGs from dead, antibiotic-resistant Pseudomonas stutzeri cells to track the movement of eARGs to live P. stutzeri cells via natural transformation, a mechanism of horizontal gene transfer involving the genomic integration of eARGs. In sterile, antibiotic-free agricultural soil, we manipulated the eARG concentration, soil moisture, and proximity to eARGs. We found that transformation occurred in soils inoculated with just 0.25 μg of eDNA g –1 soil, indicating that even low concentrations of soil eDNA can facilitate transformation (previous estimates suggested ~2 to 40 μg eDNA g –1 soil). When eDNA was increased to 5 μg g –1 soil, there was a 5-fold increase in the number of antibiotic-resistant P. stutzeri cells. We found that eARGs were transformed under soil moistures typical of terrestrial systems (5 to 30% gravimetric water content) but inhibited at very high soil moistures (>30%). Altogether, this work demonstrates that dead bacteria and their eARGs are an overlooked path to antibiotic resistance. More generally, the spread of eARGs in antibiotic-free soil suggests that transformation allows genetic variants to establish in the absence of antibiotic selection and that the soil environment plays a critical role in regulating transformation.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2024

Horizontal gene transfer of the Mer operon is associated with large effects on the transcriptome and increased tolerance to mercury in nitrogen-fixing bacteria

Abstract Background Mercury (Hg) is highly toxic and has the potential to cause severe health problems for humans and foraging animals when transported into edible plant parts. Soil rhizobia that form symbiosis with legumes may possess mechanisms to prevent heavy metal translocation from roots to shoots in plants by exporting metals from nodules or compartmentalizing metal ions inside nodules. Horizontal gene transfer has potential to confer immediate de novo adaptations to stress. We used comparative genomics of high quality de novo assemblies to identify structural differences in the genomes of nitrogen-fixing rhizobia that were isolated from a mercury (Hg) mine site that show high variation in their tolerance to Hg. Results Our analyses identified multiple structurally conserved merA homologs in the genomes of Sinorhizobium medicae and Rhizobium leguminosarum but only the strains that possessed a Mer operon exhibited 10-fold increased tolerance to Hg. RNAseq analysis revealed nearly all genes in the Mer operon were significantly up-regulated in response to Hg stress in free-living conditions and in nodules. In both free-living and nodule environments, we found the Hg-tolerant strains with a Mer operon exhibited the fewest number of differentially expressed genes (DEGs) in the genome, indicating a rapid and efficient detoxification of Hg from the cells that reduced general stress responses to the Hg-treatment. Expression changes in S. medicae while in bacteroids showed that both rhizobia strain and host-plant tolerance affected the number of DEGs. Aside from Mer operon genes, nif genes which are involved in nitrogenase activity in S. medicae showed significant up-regulation in the most Hg-tolerant strain while inside the most Hg-accumulating host-plant. Transfer of a plasmid containing the Mer operon from the most tolerant strain to low-tolerant strains resulted in an immediate increase in Hg tolerance, indicating that the Mer operon is able to confer hyper tolerance to Hg. Conclusions Mer operons have not been previously reported in nitrogen-fixing rhizobia. This study demonstrates a pivotal role of the Mer operon in effective mercury detoxification and hypertolerance in nitrogen-fixing rhizobia. This finding has major implications not only for soil bioremediation, but also host plants growing in mercury contaminated soils.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2023

Utah FORGE 1-2551: Multi-Stage Fracturing System and Well Tractor to Enable Zonal Isolation During Stimulation and EGS Operations in Horizontal Wellbores - Workshop Presentation and Report

Included here are a presentation recording, slides, and report on the Multi-Stage Fracturing System and Well Tractor to Enable Zonal Isolation During Stimulation and EGS Operations in Horizontal Wellbores project by Colorado School of Mines, presented by Dr. William W. Fleckenstein. One goal of the project is to develop and demonstrate the use of low-cost, rapid multistage fracture stimulation technology with cemented casing frac sleeves that eliminates packers used in both conventional stimulation and injection flow control. The second goal is to develop and demonstrate the devices to effectively detect and control the flow of heat-carrier fluid solely from a long-reach injector through the network of induced and existing fractures and produced from an open-hole long-reach well to improve heat recovery. This presentation was featured in the Utah FORGE R&D Annual Workshop on September 7, 2023. The workshop provided a valuable opportunity to explore the progress made in each of the 17 Research and Development projects funded under Solicitation 2020-1 which aim to enhance our understanding of the crucial factors influencing the development of Enhanced Geothermal Systems (EGS) reservoirs and resources.

15 GEOTHERMAL ENERGY↗
DOE OSTI2020

Phylogenomic Analyses of Non-Dikarya Fungi Supports Horizontal Gene Transfer Driving Diversification of Secondary Metabolism in the Amphibian Gastrointestinal Symbiont, Basidiobolus

Research into secondary metabolism (SM) production by fungi has resulted in the discovery of diverse, biologically active compounds with significant medicinal applications. The fungi rich in SM production are taxonomically concentrated in the subkingdom Dikarya, which comprises the phyla Ascomycota and Basidiomycota. Here, we explore the potential for SM production in Mucoromycota and Zoopagomycota, two phyla of nonflagellated fungi that are not members of Dikarya, by predicting and identifying core genes and gene clusters involved in SM. The majority of non-Dikarya have few genes and gene clusters involved in SM production except for the amphibian gut symbionts in the genus Basidiobolus. Basidiobolus genomes exhibit an enrichment of SM genes involved in siderophore, surfactin-like, and terpene cyclase production, all these with evidence of constitutive gene expression. Gene expression and chemical assays also confirm that Basidiobolus has significant siderophore activity. Finally, the expansion of SMs in Basidiobolus are partially due to horizontal gene transfer from bacteria, likely as a consequence of its ecology as an amphibian gut endosymbiont.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2021

Imaging the Shallow Structure of the Yucca Flat at the Source Physics Experiment Phase II Site with Horizontal-to-Vertical Spectral Ratio Inversion and a Large- N Seismic Array

The Source Physics Experiment (SPE) is a series of chemical explosions at the Nevada National Security Site (NNSS) with the goal of understanding seismic-wave generation and propagation of underground explosions. To understand explosion source physics, accurate geophysical models of the SPE site are needed. Here, we utilize a large-N seismic array deployed at the SPE phase II site to generate a shallow subsurface model of shear-wave velocity. The deployment consists of 500 geophones and covers an area of, approximately, 2.5 × 2 km. The array is located in the Yucca Flat in the northeast corner of the NNSS, Nye County, Nevada. Using ambient-noise recordings throughout the large-N seismic array, we calculate horizontal-to-vertical spectral ratios (HVSRs) across the array. We obtain 2D seismic images of shear-wave velocities across the SPE phase II site for the shallow structure of the basin. In this work the results clearly image two significant seismic impedance interfaces at ~150–500 and ~350–600 m depth. The shallower interface relates to the contrast between Quaternary alluvium and Tertiary volcanic rocks. The deeper interface relates to the contrast between Tertiary volcanic rocks and the Paleozoic bedrock. The 2D subsurface models support and extend previous understanding of the structure of the SPE phase II site. This study shows that the HVSR method in conjunction with a large-N seismic array is a quick and effective method for investigating shallow structures.

58 GEOSCIENCES↗
DOE OSTI2021

Bias Characterization, Vertical Interpolation, and Horizontal Interpolation for Distributed Wind Siting Using Mesoscale Wind Resource Estimates

Much like their counterparts in utility-scale wind energy, developers of industrial, small-scale and distributed wind turbine deployments need to understand and accurately characterize the wind resource to properly assess the power generation and financial ramifications during siting and planning. National Renewable Energy Laboratory’s WIND (Wind Integration National Dataset) Toolkit (WTK) provides a best-in-class wind resource dataset generated using the Weather Research and Forecasting (WRF) model. This dataset includes parameters such as the wind speed, wind direction, and temperature at various heights, plus atmospheric stability near the surface. This data is available at 2-km spatial resolution and five-minute temporal resolution across 7 years, from 2007 to 2013 through a publicly accessible API interface. The Tools Assessing Performance (TAP) project seeks to extend this dataset to allow long term resource estimates and leverage it to better equip distributed wind equipment manufacturers, owner-operators, and installation professionals with better tools for practical siting applications. In this report, we present the results from our investigation within the TAP project focused on characterization of bias in WTK-based wind speed estimates and evaluation of vertical and horizontal interpolation techniques. We discuss the tradeoffs between different techniques and their combinations, as well as describe the lower bounds we determine for the studied validation errors. While the specific estimates we present are specific to WTK and the validation dataset we have chosen for this investigation (NREL's Wind Resource Meteorological Database), the overall analysis and the studied techniques are general enough to be applied to a broader set of wind datasets, both simulation-based and observational.

17 WIND ENERGY↗
DOE OSTI2022

Comparison of Auxenochlorella protothecoides and Chlorella spp. Chloroplast Genomes: Evidence for Endosymbiosis and Horizontal Virus-like Gene Transfer

Resequencing of the chloroplast genome (cpDNA) of Auxenochlorella protothecoides UTEX 25 was completed (GenBank Accession no. KC631634.1), revealing a genome size of 84,576 base pairs and 30.8% GC content, consistent with features reported for the previously sequenced A. protothecoides 0710, (GenBank Accession no. KC843975). The A. protothecoides UTEX 25 cpDNA encoded 78 predicted open reading frames, 32 tRNAs, and 4 rRNAs, making it smaller and more compact than the cpDNA genome of C. variabilis (124,579 bp) and C. vulgaris (150,613 bp). By comparison, the compact genome size of A. protothecoides was attributable primarily to a lower intergenic sequence content. The cpDNA coding regions of all known Chlorella species were found to be organized in conserved colinear blocks, with some rearrangements. The Auxenochlorella and Chlorella species genome structure and composition were similar, and of particular interest were genes influencing photosynthetic efficiency, i.e., chlorophyll synthesis and photosystem subunit I and II genes, consistent with other biofuel species of interest. Phylogenetic analysis revealed that Prototheca cutis is the closest known A. protothecoides relative, followed by members of the genus Chlorella. The cpDNA of A. protothecoides encodes 37 genes that are highly homologous to representative cyanobacteria species, including rrn16, rrn23, and psbA, corroborating a well-recognized symbiosis. Several putative coding regions were identified that shared high nucleotide sequence identity with virus-like sequences, suggestive of horizontal gene transfer. Despite these predictions, no corresponding transcripts were obtained by RT-PCR amplification, indicating they are unlikely to be expressed in the extant lineage.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2022

A Novel Trichothecene Toxin Phenotype Associated with Horizontal Gene Transfer and a Change in Gene Function in Fusarium

Fusarium trichothecenes are among the mycotoxins of most concern to food and feed safety. Production of these mycotoxins and presence of the trichothecene biosynthetic gene (TRI) cluster have been confirmed in only two multispecies lineages of Fusarium: the Fusarium incarnatum-equiseti (Incarnatum) and F. sambucinum (Sambucinum) species complexes. Here, we identified and characterized a TRI cluster in a species that has not been formally described and is represented by Fusarium sp. NRRL 66739. This fungus is reported to be a member of a third Fusarium lineage: the F. buharicum species complex. Cultures of NRRL 66739 accumulated only two trichothecenes, 7-hydroxyisotrichodermin and 7-hydroxyisotrichodermol. Although these are not novel trichothecenes, the production profile of NRRL 66739 is novel, because in previous reports 7-hydroxyisotrichodermin and 7-hydroxyisotrichodermol were components of mixtures of 6–8 trichothecenes produced by several Fusarium species in Sambucinum. Heterologous expression analysis indicated that the TRI13 gene in NRRL 66739 confers trichothecene 7-hydroxylation. This contrasts the trichothecene 4-hydroxylation function of TRI13 in other Fusarium species. Phylogenetic analyses suggest that NRRL 66739 acquired the TRI cluster via horizontal gene transfer from a close relative of Incarnatum and Sambucinum. These findings provide insights into evolutionary processes that have shaped the distribution of trichothecene production among Fusarium species and the structural diversity of the toxins.

Food Science & Technology↗
DOE OSTI2022

The SDSS-Gaia View of the Color–Magnitude Relation for Blue Horizontal-branch Stars

We present an updated sample of blue horizontal-branch (BHB) stars selected from the photometric and spectroscopic data from Sloan Digital Sky Survey and its associated project Sloan Extension for Galactic Understanding and Exploration (SEGUE). With these data, we selected candidates for A-type stars in the color–color space and then a mixture modeling technique was implemented in order to distinguish between BHB and main-sequence/blue-straggler stars based on their surface gravity values (log g) estimated by the SEGUE Stellar Parameter Pipeline. Our robust approach allows us to attribute individual probabilities of each star truly being in the BHB stage. Hence, our method is advantageous in comparison to previous SEGUE BHB selections that adopted simple log g cuts. We also revisit the color–magnitude relation for these stars and propose two calibrations, based on updated distances for Galactic globular clusters, to estimate absolute magnitudes with (g – r) 0 and (u – r) 0 colors.

79 ASTRONOMY AND ASTROPHYSICS↗