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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 271 records · Page 15
All sourcesNASA NTRSDOE OSTI
NASA NTRS2000

Caveolin-1 regulates shear stress-dependent activation of extracellular signal-regulated kinase

Fluid shear stress activates a member of the mitogen-activated protein (MAP) kinase family, extracellular signal-regulated kinase (ERK), by mechanisms dependent on cholesterol in the plasma membrane in bovine aortic endothelial cells (BAEC). Caveolae are microdomains of the plasma membrane that are enriched with cholesterol, caveolin, and signaling molecules. We hypothesized that caveolin-1 regulates shear activation of ERK. Because caveolin-1 is not exposed to the outside, cells were minimally permeabilized by Triton X-100 (0.01%) to deliver a neutralizing, polyclonal caveolin-1 antibody (pCav-1) inside the cells. pCav-1 then bound to caveolin-1 and inhibited shear activation of ERK but not c-Jun NH(2)-terminal kinase. Epitope mapping studies showed that pCav-1 binds to caveolin-1 at two regions (residues 1-21 and 61-101). When the recombinant proteins containing the epitopes fused to glutathione-S-transferase (GST-Cav(1-21) or GST-Cav(61-101)) were preincubated with pCav-1, only GST-Cav(61-101) reversed the inhibitory effect of the antibody on shear activation of ERK. Other antibodies, including m2234, which binds to caveolin-1 residues 1-21, had no effect on shear activation of ERK. Caveolin-1 residues 61-101 contain the scaffolding and oligomerization domains, suggesting that binding of pCav-1 to these regions likely disrupts the clustering of caveolin-1 or its interaction with signaling molecules involved in the shear-sensitive ERK pathway. We suggest that caveolae-like domains play a critical role in the mechanosensing and/or mechanosignal transduction of the ERK pathway.

Non-NASA Center↗
NASA NTRS1999

T cell activation responses are differentially regulated during clinorotation and in spaceflight

Studies of T lymphocyte activation with mitogenic lectins during spaceflight have shown a dramatic inhibition of activation as measured by DNA synthesis at 72 h, but the mechanism of this inhibition is unknown. We have investigated the progression of cellular events during the first 24 h of activation using both spaceflight microgravity culture and a ground-based model system that relies on the low shear culture environment of a rotating clinostat (clinorotation). Stimulation of human peripheral blood mononuclear cells (PBMCs) with soluble anti-CD3 (Leu4) in clinorotation and in microgravity culture shows a dramatic reduction in surface expression of the receptor for IL-2 (CD25) and CD69. An absence of bulk RNA synthesis in clinorotation indicates that stimulation with soluble Leu4 does not induce transition of T cells from G0 to the G1 stage of the cell cycle. However, internalization of the TCR by T cells and normal levels of IL-1 synthesis by monocytes indicate that intercellular interactions that are required for activation occur during clinorotation. Complementation of TCR-mediated signaling by phorbol ester restores the ability of PBMCs to express CD25 in clinorotation, indicating that a PKC-associated pathway may be compromised under these conditions. Bypassing the TCR by direct activation of intracellular pathways with a combination of phorbol ester and calcium ionophore in clinorotation resulted in full expression of CD25; however, only partial expression of CD25 occurred in microgravity culture. Though stimulation of purified T cells with Bead-Leu4 in microgravity culture resulted in the engagement and internalization of the TCR, the cells still failed to express CD25. When T cells were stimulated with Bead-Leu4 in microgravity culture, they were able to partially express CD69, a receptor that is constitutively stored in intracellular pools and can be expressed in the absence of new gene expression. Our results suggest that the inhibition of T cell proliferative response in microgravity culture is a result of alterations in signaling events within the first few hours of activation, which are required for the expression of important regulatory molecules.

NASA Experiment Number 9403079 1/2↗
NASA NTRS1997

CCAAT/enhancer-binding protein delta activates insulin-like growth factor-I gene transcription in osteoblasts. Identification of a novel cyclic AMP signaling pathway in bone

Insulin-like growth factor-I (IGF-I) plays a key role in skeletal growth by stimulating bone cell replication and differentiation. We previously showed that prostaglandin E2 (PGE2) and other cAMP-activating agents enhanced IGF-I gene transcription in cultured primary rat osteoblasts through promoter 1, the major IGF-I promoter, and identified a short segment of the promoter, termed HS3D, that was essential for hormonal regulation of IGF-I gene expression. We now demonstrate that CCAAT/enhancer-binding protein (C/EBP) delta is a major component of a PGE2-stimulated DNA-protein complex involving HS3D and find that C/EBPdelta transactivates IGF-I promoter 1 through this site. Competition gel shift studies first indicated that a core C/EBP half-site (GCAAT) was required for binding of a labeled HS3D oligomer to osteoblast nuclear proteins. Southwestern blotting and UV-cross-linking studies showed that the HS3D probe recognized a approximately 35-kDa nuclear protein, and antibody supershift assays indicated that C/EBPdelta comprised most of the PGE2-activated gel-shifted complex. C/EBPdelta was detected by Western immunoblotting in osteoblast nuclear extracts after treatment of cells with PGE2. An HS3D oligonucleotide competed effectively with a high affinity C/EBP site from the rat albumin gene for binding to osteoblast nuclear proteins. Co-transfection of osteoblast cell cultures with a C/EBPdelta expression plasmid enhanced basal and PGE2-activated IGF-I promoter 1-luciferase activity but did not stimulate a reporter gene lacking an HS3D site. By contrast, an expression plasmid for the related protein, C/EBPbeta, did not alter basal IGF-I gene activity but did increase the response to PGE2. In osteoblasts and in COS-7 cells, C/EBPdelta, but not C/EBPbeta, transactivated a reporter gene containing four tandem copies of HS3D fused to a minimal promoter; neither transcription factor stimulated a gene with four copies of an HS3D mutant that was unable to bind osteoblast nuclear proteins. These results identify C/EBPdelta as a hormonally activated inducer of IGF-I gene transcription in osteoblasts and show that the HS3D element within IGF-I promoter 1 is a high affinity binding site for this protein.

NASA Discipline Musculoskeletal↗
NASA NTRS2018

Discovery of Activities via Statistical Clustering of Fixation Patterns

Human behavior often consists of a series of distinct activities, each characterized by a unique pattern of interaction with the visual environment. This is true even in a restricted domain, such as a piloting an aircraft, where activities with distinct visual signatures might be things like communicating, navigating, and monitoring. We propose a novel analysis method for gaze-tracking data, to perform blind discovery of these hypothetical activities. The method is in some respects similar to recurrence analysis, but here we compare not individual fixations, but groups of fixations aggregated over a fixed time interval. The duration of this interval is a parameter that we will refer to as delta. We assume that the environment has been divided into a set of N different areas-of-interest (AOIs). For a given interval of time of duration delta, we compute the proportion of time spent fixating each AOI, resulting in an N-dimensional vector. These proportions can be converted to integer counts by multiplying by delta divided by the average fixation duration (another parameter that we fix at 280 milliseconds). We compare different intervals by computing the chi-square statistic. The p-value associated with the statistic is the likelihood of observing the data under the hypothesis that the data in the two intervals were generated by a single process with a single set of probabilities governing the fixation of each AOI. The method has been applied to approximately 100 hours of eye movement data collected from pilots in a high-fidelity B747 flight simulator, and the results have been compared to synthetic data in which the each activity is represented as first-order Markov process with random probabilities assigned to the AOIs. Randomly-generated synthetic activities can require thousands of fixations to be discriminated with statistical significance, while the human data can be clustered using averaging windows of some 10's of seconds, suggesting that the actual activities are much more narrowly focused than random Markov models.

activity analysis↗
NASA NTRS2021

A Comparison of the CIR- and CME-Induced Geomagnetic Activity Effects on Mesosphere and Lower Thermospheric Temperature

Neutral temperature responses in the mesosphere and lower thermosphere (MLT) to severe geomagnetic storms induced by coronal mass ejections (CMEs) are of growing interest to the space science research community. Recently, it was found that geomagnetic activities produced by the corotating interaction regions (CIRs) caused comparable effects on the Earth's upper atmosphere. In this work, we carried out a comparative study of the temperature responses in the MLT region to these two types of geomagnetic activities, using the temperature measured by the Sounding of the Atmosphere using Broadband Emission Radiometry (SABER) instruments onboard the Thermosphere, Ionosphere, Mesosphere Energetics and Dynamics (TIMED) satellite. Our results demonstrate that CIR-induced geomagnetic activity produced temperature variations in the MLT region and that this effect can penetrate downward to ∼100 km at high latitudes in both hemispheres. Temperature enhancements penetrated deeper during CME-induced geomagnetic activities, but the heating effects lasted longer during CIR-induced geomagnetic activities. There is a hemispherical asymmetry in the geomagnetical activity induced temperature changes in the MLT region. The temperature enhancements are stronger in the southern hemisphere than in the northern hemisphere during CME events.

geomagnetic activity↗
NASA NTRS1973

The activation energy for creep of columbium /niobium/.

The activation energy for creep of nominally pure columbium (niobium) was determined in the temperature range from 0.4 to 0.75 T sub M by measuring strain rate changes induced by temperature shifts at constant stress. A peak in the activation energy vs temperature curve was found with a maximum value of 160 kcal/mole. A pretest heat treatment of 3000 F for 30 min resulted in even higher values of activation energy (greater than 600 kcal/mole) in this temperature range. The activation energy for the heat-treated columbium (Nb) could not be determined near 0.5 T sub M because of unusual creep curves involving negligible steady-state creep rates and failure at less than 5% creep strain. It is suggested that the anomalous activation energy values and the unusual creep behavior in this temperature range are caused by dynamic strain aging involving substitutional atom impurities and that this type of strain aging may be in part responsible for the scatter in previously reported values of activation energy for creep of columbium (Nb) near 0.5 T sub M.

Klein, M. J.↗
NASA NTRS1974

Flutter suppression and gust alleviation using active controls

The effects of active controls on the suppression of flutter and gust alleviation of two different types of subsonic aircraft (the Arava, twin turboprop STOL transport, and the Westwind twin-jet business transport) are investigated. The active controls are introduced in pairs which include, in any chosen wing strip, a leading-edge (LE) control and a trailing-edge (TE) control. Each control surface is allowed to be driven by a combined linear-rotational sensor system, located on the activated strip. The control law, which translates the sensor signals into control surface rotations, is based on the concept of aerodynamic energy. The results indicate the extreme effectiveness of the active systems in controlling flutter. A single system spanning 10% of the wing semispan made the Arava flutter-free, and a similar active system, for the Westwind aircraft, yielded a reduction of 75% in the maximum bending moment of the wing and a reduction of 90% in the acceleration of the cg of the aircraft. Results for simultaneous activation of several LE - TE systems are presented. Further work needed to bring the investigation to completion is also discussed.

Nissim, E.↗
NASA NTRS1973

The evolution of prominences and their relationship to active centers /A Review/

A necessary condition for the formation of prominences is established where components of the magnetic field of opposite sign lie juxtaposed. This condition is sometimes recognizable prior to the formation of prominences in active centers and between adjacent active centers in H-alpha by paths of fibrils aligned nearly end to end. Prominences are usually not found in active regions until they are about 4 days old. After this time, the number of prominences appears to be a function of the large-scale complexities in the magnetic field pattern whether these are a result of emerging flux, the merging of adjacent active centers, or the coalescing of remnant fields of active centers. Prominences may disappear by the slow or rapid flow of mass into the chromosphere or by the more violent eruptives. The formation of new active centers has been associated with the eruption of some filaments related to weak chromospheric fields.

Martin, S. F.↗
NASA NTRS1976

JPL Activated Carbon Treatment System (ACTS) for sewage

An Activated Carbon Treatment System (ACTS) was developed for sewage treatment and is being applied to a one-million gallon per day sewage treatment pilot plant in Orange County California. Activities reported include pyrolysis and activation of carbon-sewage sludge, and activated carbon treatment of sewage to meet ocean discharge standards. The ACTS Sewage treatment operations include carbon-sewage treatment, primary and secondary clarifiers, gravity (multi-media) filter, filter press dewatering, flash drying of carbon-sewage filter cake, and sludge pyrolysis and activation. Tests were conducted on a laboratory scale, 10,000 gallon per day demonstration plant and pilot test equipment. Preliminary economic studies are favorable to the ACTS process relative to activated sludge treatment for a 175,000,000 gallon per day sewage treatment plant.

Source record↗
NASA NTRS1976

Application of the aerodynamic energy concept to flutter suppression and gust alleviation by use of active controls

The effects of active controls on flutter suppression and gust alleviation of the Arava twin turboprop STOL transport and the Westwind twinjet business transport are investigated. The active control surfaces are introduced in pairs which include, in any chosen wing strip, a 20-percent chord leading-edge control and a 20-percent chord trailing-edge control. Each control surface is driven by a combined linear-rotational sensor system located on the activated strip. The control law is based on the concept of aerodynamic energy and utilizes previously optimized control law parameters based on two-dimensional aerodynamic theory. The best locations of the activated system along the span of the wing are determined for bending-moment alleviation, reduction in fuselage accelerations, and flutter suppression. The effectiveness of the activated system over a wide range of maximum control deflections is also determined. Two control laws are investigated. The first control law utilizes both rigid-body and elastic contributions of the motion. The second control law employs primarily the elastic contribution of the wing and leads to large increases in the activated control effectiveness as compared with the basic control law. The results indicate that flutter speed can be significantly increased (over 70 percent increase) and that the bending moment due to gust loading can be almost totally eliminated by a control system of about 10 to 20 percent span with reasonable control-surface rotations.

Nissim, E.↗
NASA NTRS1977

Preliminary investigation of a sealed, remotely activated silver-zinc battery

Methods necessary to provide a remotely activated, silver zinc battery capable of an extended activated stand while in a sealed condition were investigated. These requirements were to be accomplished in a battery package demonstrating an energy density of at least 35 watt hours per pound. Several methods of gas suppression were considered in view of the primary nature of this unit and utilized the electroplated dendritic zinc electrode. Amalgamation of the electrode provided the greatest suppression of gas at the zinc electrode. The approach to extending the activated stand capability of the remotely activated battery was through evaluation of three basic methods of remote, multi-cell activation; 1) the electrolyte manifold, 2) the gas manifold and 3) the individual cell. All three methods of activation can be incorporated into units which will meet the minimum energy density requirement.

Wheat, C. G.↗
NASA NTRS1977

Open magnetic fields in active regions

Soft X-ray images and magnetograms of several active regions and coronal holes are examined which support the interpretation that some of the dark X-ray gaps seen between interconnecting loops and inner cores of active regions are foot points of open field lines inside the active regions. Characteristics of the investigated dark gaps are summarized. All the active regions with dark X-ray gaps at the proper place and with the correct polarity predicted by global potential extrapolation of photospheric magnetic fields are shown to be old active regions, indicating that field opening is accomplished only in a late phase of active-region development. It is noted that some of the observed dark gaps probably have nothing in common with open fields, but are either due to the decreased temperature in low-lying portions of interconnecting loops or are the roots of higher and less dense or cooler loops.

Svestka, Z.↗
NASA NTRS1979

Accelerated development and flight evaluation of active controls concepts for subsonic transport aircraft

The flight test of an active load alleviation/extended span for the L-1011 wide-body transport aircraft, and piloted simulation work leading to use of active stability augmentation with a small tail and aft center of gravity are reported. The extended span showed the expected cruise drag reduction of 3%. The small tail is expected to reduce cruise drag by another 3%, and eventual use of more aft center of gravity with active stability augmentation will provide further fuel savings. The active load alleviation functions included maneuver load control (MLC) and elastic mode suppression (EMS), using symmetric motions of the outboard ailerons to reduce wing bending loads in maneuvers or long-term up- or down-drafts (MLC), and to damp wing bending motions in turbulence (EMS). A gust load alleviation function using the active horizontal tail to provide airplane pitch damping in turbulence was found unnecessary. The piloted simulation tests evaluated criteria for augmentation-on and augmentation-off flying qualities. of a simple pitch control law was verified at neutral static margin. The simulation tasks established the basis for follow-on construction and flight testing of a small tail with active stability augmentation.

Source record↗
NASA NTRS1979

Review of selected geomagnetic activity indices

Magnetic activity indexes are reviewed. Classifications of magnetograms from single observatories and the global range of potential associated with the equivalent currents which could have produced the variations monitored at a large array of recording sides are addressed. Principal magnetic activity indexes discussed include: the auroral electrojet index and its associated indexes (AU, AL and AO) useful for auroral zone studies; the Kp, ap, aa and am indexes which are measures of midlatitude geomagnetic activity; and the Dst index of magnetic activity recorded at low latitudes. It is concluded that geomagnetic activity indexes are useful in studies of the interaction between solar activity, the interplanetary magnetic field and solar wind, the magnetosphere, ring current, field aligned currents, and ionospheric currents.

Allen, J. H.↗
NASA NTRS1981

A global tectonic activity map with orbital photographic supplement

A three part map showing equatorial and polar regions was compiled showing tectonic and volcanic activity of the past one million years, including the present. Features shown include actively spreading ridges, spreading rates, major active faults, subduction zones, well defined plates, and volcanic areas active within the past one million years. Activity within this period was inferred from seismicity (instrumental and historic), physiography, and published literature. The tectonic activity map was used for planning global geodetic programs of satellite laser ranging and very long base line interferometry and for geologic education.

Lowman, P. D., Jr.↗
NASA NTRS1982

Studies of neutron and proton nuclear activation in low-Earth orbit

The expected induced radioactivity of experimental material in low Earth orbit was studied for characteristics of activating particles such as cosmic rays, high energy Earth albedo neutrons, trapped protons, and secondary protons and neutrons. The activation cross sections for the production of long lived radioisotopes and other existing nuclear data appropriate to the study of these reactions were compiled. Computer codes which are required to calculate the expected activation of orbited materials were developed. The decreased computer code used to predict the activation of trapped protons of materials placed in the expected orbits of LDEF and Spacelab II. Techniques for unfolding the fluxes of activating particles from the measured activation of orbited materials are examined.

Laird, C. E.↗
NASA NTRS1983

Magnetic fields and activity of the sun and stars - An overview

Recent work on the observation and theory of solar and stellar magnetic field activity and its relation to stellar activity is reviewed, emphasizing those aspects relevant to the problem of activity of red dwarf stars. New observational facts relevant to understanding the root cause of stellar surface activity are summarized and theoretical questions concerning the underlying physical basis for the observed correlations between stellar activity, rotation, and magnetic fields are addressed. These include dyanamo theory and the rotation-activity connection as well as flux tube dynamics and plasma heating.

Rosner, R.↗
NASA NTRS1985

Method for measuring surface activity of silicon nitride powder

Amorphous, alpha-, and beta-Si3N4 powders were activated by vibration ball milling in purified MeOH, and the surface activity of ground powders was determined by the temperature programmed desorption (TPD) method using NH3 gas. The concentration of active sites with a potential energy equivalent to the peak temperature in the spectrum increased was markedly by ball milling the amorphous Si3N4. The alpha- and beta-Si3N4 also had active sites produced by ball milling. The concentration of active site increased with increased ball milling time. A method for measuring surface activity of ceramic raw materials by TPD is proposed.

Kanno, Y.↗