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At least 253 records · Page 14
All sourcesNASA NTRSDOE OSTI
DOE OSTI2023

Polymer-based microfluidic device for on-chip counter-diffusive crystallization and in situ X-ray crystallography at room temperature

Proteins are long chains of amino acid residues that perform a myriad of functions in living organisms, including enzymatic reactions, signalling, and maintaining structural integrity. Protein function is determined directly by the protein structure. X-ray crystallography is the primary technique for determining the 3D structure of proteins, and facilitates understanding the effects of protein structure on function. The first step towards structure determination is crystallizing the protein of interest. We have developed a centrifugally-actuated microfluidic device that incorporates the fluid handling and metering necessary for protein crystallization. Liquid handling takes advantage of surface forces to control fluid flow and enable metering, without the need for any fluidic or pump connections. Our approach requires only the simple steps of pipetting the crystallization reagents into the device followed by either spinning or shaking to set up counter-diffusive protein crystallization trials. The use of thin, UV-curable polymers with a high level of X-ray transparency allows for in situ X-ray crystallography, eliminating the manual handling of fragile protein crystals and streamlining the process of protein structure analysis. We demonstrate the utility of our device using hen egg white lysozyme as a model system, followed by the crystallization and in situ, room temperature structural analysis of the hub domain of calcium–calmodulin dependent kinase II (CaMKIIβ).

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗
NASA NTRS1974

Erosion by catastrophic floods on Mars and Earth

The morphologic features of the large Martian channels are shown to be strikingly similar to those of the Channeled Scabland of eastern Washington, produced by the catastrophic breakout floods of Pleistocene Lake Missoula. If the analogy is correct, floods involving water discharges of millions of cubic meters per second and peak flow velocities of tens of meters per second, but perhaps lasting no more than a few days, may have occurred on Mars.

Baker, V. R.↗
NASA NTRS2012

A Volcanic Origin for Sinuous and Branching Channels on Mars: Evidence from Hawaiian Analogs

Observations of sinuous and branching channels on planets have long driven a debate about their origin, fluvial or volcanic processes. In some cases planetary conditions rule out fluvial activity (e.g. the Moon, Venus, Mercury). However, the geology of Mars leads to suggestions that liquid water existed on the surface in the past. As a result, some sinuous and branching channels on Mars are cited as evidence of fluvial erosion. Evidence for a fluvial history often focuses on channel morphologies that are unique from a typical lava channel, for instance, a lack of detectable flow margins and levees, islands and terraces. Although these features are typical, they are not necessarily diagnostic of a fluvial system. We conducted field studies in Hawaii to characterize similar features in lava flows to better define which characteristics might be diagnostic of fluvial or volcanic processes. Our martian example is a channel system that originates in the Ascraeus Mons SW rift zone from a fissure. The channel extends for approx.300 km to the SE/E. The proximal channel displays multiple branches, islands, terraces, and has no detectable levees or margins. We conducted field work on the 1859 and 1907 Mauna Loa flows, and the Pohue Bay flow. The 51-km-long 1859 Flow originates from a fissure and is an example of a paired a a and pahoehoe lava flow. We collected DGPS data across a 500 m long island. Here, the channel diverted around a pre-existing obstruction in the channel, building vertical walls up to 9 m in height above the current channel floor. The complicated emplacement history along this channel section, including an initial a a stage partially covered by pahoehoe overflows, resulted in an appearance of terraced channel walls, no levees and diffuse flow margins. The 1907 Mauna Loa flow extends > 20 km from the SW rift zone. The distal flow formed an a a channel. However the proximal flow field comprises a sheet that experienced drainage and sagging of the crust following the eruption. The lateral margins of the proximal sheet, past which all lava flowed to feed the extensive channel, currently display a thickness of < 20 cm. Were this area covered by a dust layer, as is the Tharsis region on Mars, the margins would be difficult to identify. The Pohue Bay flow forms a lava tube. Open roof sections experienced episodes of overflow and spill out. In several places the resultant surface flows appear to have moved as sheet flows that inundated the preexisting meter scale features. Here the flows developed pathways around topographic highs, and in so doing accreted lava onto those features. The results are small islands within the multiple branched channels that display steep, sometimes overhanging walls. None of these features alone proves that the martian channel networks are the result of volcanic processes, but analog studies such as these are the first step towards identifying which morphologies are truly diagnostic of fluvial and volcanic channels.

Bleacher, Jacob E.↗
NASA NTRS1985

Multiscale modeling to evaluate proposed space-based Doppler lidar sampling strategies

A proposal has been made to place a pulsed Doppler lidar on a space platform (Huffaker, et al., 1980; Emmitt, 1982) in a low earth orbit (200 to 800 km) to measure the atmospheric winds with a spatial resolution commensurate with the current continental rawinsonde network density - i.e., 300 to 500 km resolution. In the case of the space-based doppler lidar, the full range of space scales applies. Single shot pulses with dimensions of 10 x 1000 meters are used to sample areas 100,000 x 100,000 meters to resolved mass flow structure with wavelength of 1 million meters. Simulation studies, therefore, require an equally broad range of atmospheric models. A general circulation model is appropriate to answer questions regarding the impact of a global wind measuring system upon synoptic forecasts. Since the nominal resolution of the spacebased system is expected to be a few 100's of kilometers, then a numerical model with mesoscale dynamics is required. The meaning of an average Doppler shift within a laser pulse volume must be evaluated with models of turbulent/convective scale motions and aerosol gradients. Examples of how models on all these scales have been applied in an ongoing simulation study are presented. In particular, the uncertainties in a mesoscale wind estimate are separated into those arising from pulse scale variances and those due to sample distribution within a prescribed resolution volume. Trade-offs between accuracy and representativeness are discussed in terms of the model results.

Emmitt, G. D.↗
NASA NTRS1984

Flow improvements in the NASA Langley 4- by 7-meter tunnel circuit

The mean velocity profiles in both the horizontal and vertical planes of symmetry at specific locations throughout the tunnel circuit to identify the most promising means for improving the flow in the 4 by 7 meter wind tunnel were measured. In the base line tunnel flow surveys, the flow patterns near the end of the test section indicate a uniform mean velocity distribution. Downstream of the test section, unsymmetrical flow patterns result in low velocities along the inner walls and in flow separation along the inner wall of the diffuser upstream of the drive fan and along the outer wall of the large diffuser downstream of the drive fan. A set of trailing-edge flaps attached to the five flow-control vanes located just downstream of the first corner were installed. These flaps are successful in making the tunnel flow more symmetrical and in eliminating the regions of separation in the diffusers upstream and downstream of the drive fan. Previously announced in STAR as N84-15117

Applin, Z. T.↗
NASA NTRS1982

Velocity and flow angle measurements in the Langley 0.3-meter transonic cryogenic tunnel using a laser transit anemometer

The Laser Transit Anemometer (LTA) system is described. In the LTA system two parallel laser beams of known separation and cross sectional area are focussed at the same location or plane. When a particle in a flow field passes through both beams and the time is recorded for its transit (time of flight), its velocity can be calculated knowing the distance between the beams. By rotating the two beams (spots) around a common center and recording the number of valid events (a particle which passes through both spots in the proper sequence) at each angle the flow angle can be determined by curve fitting a predetermined number of angles or points and calculating the peak of what should be a Gaussian curve. The best angle or flow angle is defined as the angle at which the maximum number of valid events occurs. The LTA system functioned properly although conditions were less than desirable.

Honaker, W. C.↗
NASA NTRS2017

Weather Impact on Airport Arrival Meter Fix Throughput

Time-based flow management provides arrival aircraft schedules based on arrival airport conditions, airport capacity, required spacing, and weather conditions. In order to meet a scheduled time at which arrival aircraft can cross an airport arrival meter fix prior to entering the airport terminal airspace, air traffic controllers make regulations on air traffic. Severe weather may create an airport arrival bottleneck if one or more of airport arrival meter fixes are partially or completely blocked by the weather and the arrival demand has not been reduced accordingly. Under these conditions, aircraft are frequently being put in holding patterns until they can be rerouted. A model that predicts the weather impacted meter fix throughput may help air traffic controllers direct arrival flows into the airport more efficiently, minimizing arrival meter fix congestion. This paper presents an analysis of air traffic flows across arrival meter fixes at the Newark Liberty International Airport (EWR). Several scenarios of weather impacted EWR arrival fix flows are described. Furthermore, multiple linear regression and regression tree ensemble learning approaches for translating multiple sector Weather Impacted Traffic Indexes (WITI) to EWR arrival meter fix throughputs are examined. These weather translation models are developed and validated using the EWR arrival flight and weather data for the period of April-September in 2014. This study also compares the performance of the regression tree ensemble with traditional multiple linear regression models for estimating the weather impacted throughputs at each of the EWR arrival meter fixes. For all meter fixes investigated, the results from the regression tree ensemble weather translation models show a stronger correlation between model outputs and observed meter fix throughputs than that produced from multiple linear regression method.

Machine Learning Model.↗
NASA NTRS1983

Flow improvements in the circuit of the Langley 4- by 7-meter tunnel

In order to identify the most promising means for improving the flow in the Langley 4- by 7-Meter Tunnel, detailed surveys of the mean velocity profiles were measured in both the horizontal and vertical planes of symmetry at specific locations throughout the tunnel circuit. These data were obtained at test-section dynamic pressures of 1.53, 2.78, and 4.79 kPa (3 2, 58, and 100 psf, respectively) for tunnel operation in both the closed and open test-section configurations. In the base-line tunnel-flow surveys, the flow patterns near the end of the test section indicate a uniform mean velocity distribution. Downstream of the test section, unsymmetrical flow patterns result in low velocities along the inner walls and in flow separation along the inner wall of the diffuser upstream of the drive fan and along the outer wall of the large diffuser downstream of the drive fan. In order to correct these unsymmetrical flow patterns, a set of trailing-edge flaps attached to the five flow-control vanes located just downstream of the first corner were installed. These flaps were very successful in making the tunnel flow more symmetrical and in eliminating the regions of separation in the diffusers upstream and downstream of the drive fan.

Zachary T Applin↗
NASA NTRS2004

Liquid Metal Propellant Feed System for Plasma Propulsion

High-power plasma thrusters that utilize molten metallic propellants (e.g., the Lithium Lorentz Force Accelerator) are currently being investigated as a primary propulsion option for in-space nuclear-electric systems. A critical component of the thruster is the propellant feed system, which must reliably and accurately pump liquid metal into the thruster discharge chamber. We present design details and calibration results for a compact liquid metal propellant feed system that contains no moving parts, for use in laboratory testing of plasma thrusters. Feed line pressure is maintained using an MHD flow coupler, and the flow rate is monitored using a simple voltage divider, which is submerged in the propellant reservoir. Results for lithium and gallium propellants show capability to meter propellant at flow rates up to 10 +/- 0.1 mg/s.

Markusic, T. E.↗
NASA NTRS1989

Whistle Gauge Measures Flow And Temperature

Simple, rugged gauge used to measure speed of flow and temperature of steam or other gas flowing through pipes of arbitrary diameter, from 1 to 28 in. or larger. Specially designed, instrumented whistle - has no moving parts, small, nonobstruction, operates at high temperature and pressure, and cleans itself. Does not operate at zero flow, but at moderate flows (tens of meters per second) generates intense sound for use in measurements. Consists of slanted ring groove of depth D and pressure taps in wall of pipe carrying flow to be measured. Resonant wavelength of sound generated by ring groove depends primarily on size and shape of groove and approximately equal to 4D.

Shakkottai, Parthasarathy↗
DOE OSTI2022

Influence of sequential stimulation practices on geochemical alteration of shale

Water-based hydraulic fracturing fluids (HFFs) can chemically interact with formation shale, resulting in altered porosity and permeability of the host rock. Experimental investigations of spatial and temporal shale-HFF interactions are helpful in interpreting chemical compositions of the injectate, as well as predicting alteration of hydraulic properties in the reservoir due to mineral dissolution and precipitation. Most bench-top experiments designed to study shale-HFF chemical interactions, either using batch reactors or flow-through setups, are carried out assuming that the acid spearhead has already become mixed with neutral HFFs. During operations, however, HFFs are typically injected according to a sequenced pumping schedule, starting with a concentrated acid spearhead, followed by multiple additions of near-neutral pH HFFs containing chemical amendments and proppant. In this study, we use geochemical modeling to consider whether this pre-mixed experimental protocol provides results directly comparable to a sequential discrete fluid-shale interaction protocol. Our results show that for the batch system, the transient evolution in major ion concentrations is faster with the sequential procedure. After 2 h of reaction time, the two protocols converge to the same aqueous concentrations. In a flow-through geometry, the pre-mixed model predicts extensive chemical alteration close to the injection point but negligible alteration downstream. In contrast, the sequential model predicts mineral reactions over hundreds of meters along the flow path. The extent of shale alteration in the sequential model at a given location depends on shale mineralogy and where the acid spearhead resides during the shut-in period. The predictive model developed in this study can help experimentalists to design bench-top tests and operators to better translate the results of laboratory experiments into practical applications.

Li, Qingyun↗
NASA NTRS2003

Real-time three-dimensional color doppler evaluation of the flow convergence zone for quantification of mitral regurgitation: Validation experimental animal study and initial clinical experience

BACKGROUND: Pitfalls of the flow convergence (FC) method, including 2-dimensional imaging of the 3-dimensional (3D) geometry of the FC surface, can lead to erroneous quantification of mitral regurgitation (MR). This limitation may be mitigated by the use of real-time 3D color Doppler echocardiography (CE). Our objective was to validate a real-time 3D navigation method for MR quantification. METHODS: In 12 sheep with surgically induced chronic MR, 37 different hemodynamic conditions were studied with real-time 3DCE. Using real-time 3D navigation, the radius of the largest hemispherical FC zone was located and measured. MR volume was quantified according to the FC method after observing the shape of FC in 3D space. Aortic and mitral electromagnetic flow probes and meters were balanced against each other to determine reference MR volume. As an initial clinical application study, 22 patients with chronic MR were also studied with this real-time 3DCE-FC method. Left ventricular (LV) outflow tract automated cardiac flow measurement (Toshiba Corp, Tokyo, Japan) and real-time 3D LV stroke volume were used to quantify the reference MR volume (MR volume = 3DLV stroke volume - automated cardiac flow measurement). RESULTS: In the sheep model, a good correlation and agreement was seen between MR volume by real-time 3DCE and electromagnetic (y = 0.77x + 1.48, r = 0.87, P <.001, delta = -0.91 +/- 2.65 mL). In patients, real-time 3DCE-derived MR volume also showed a good correlation and agreement with the reference method (y = 0.89x - 0.38, r = 0.93, P <.001, delta = -4.8 +/- 7.6 mL). CONCLUSIONS: real-time 3DCE can capture the entire FC image, permitting geometrical recognition of the FC zone geometry and reliable MR quantification.

Validation Studies↗
DOE OSTI2022

Scale-Up and Modeling Efforts Using an Omniphobic Surface Treatment for Mitigating Solids Deposition

Abstract Gas hydrates, waxes and asphaltenes represent some of the most significant flow assurance challenges, especially in subsea lines, where treatment options can be limited. Currently, complete avoidance is the primary strategy for hydrate management, while chemical or mechanical flushing/pigging may be utilized for other solids. Each system must also be treated with individualized solutions, as there have been no proved one-size-fits-all technologies demonstrated to date. As an alternative to constant chemical injection or thermodynamic controls such as insulation or heating, a robust omniphobic surface treatment material has been developed which has been shown in previous studies to significantly reduce the adhesion of flow assurance solids, resulting in lower risk for deposition and plugging of gas hydrates, waxes, and asphaltenes. As part of a Department of Energy study, laboratory scale tests were performed on a variety of apparatuses ranging from micromechanical force (micron scale) to laboratory flow loop testing (meter scale). While the results from these tests have been promising, there can be some disconnects between lab-scale observations and field-scale testing. Several factors can result in discrepancies between small-scale bench-top studies and deployment-scale efforts. These include flow conditions (Reynolds number, flow regime), chemistry (synthetic vs. crude oils, gas composition, and additives), physical differences (pipeline material, size, and geometry), etc. This work will describe the actions taken to better understand the deployment and performance of a material such as this omniphobic surface treatment in field scenarios, and to build confidence for deployment in a production scenario. These efforts include modeling field production scenarios using models developed from lab observations, application of the material to field-scale equipment to better understand application challenges, and expanded survivability and longevity testing. Understanding the bridge between laboratory testing and full-scale deployment allows for better technique and risk mitigation for field scale testing.

03 NATURAL GAS↗
NASA NTRS2005

Electromagnetic Pumps for Conductive-Propellant Feed Systems

There has been a recent, renewed interest in high-power electric thrusters for application in nuclear-electric propulsion systems. Two of the most promising thrusters utilize liquid metal propellants: the lithium-fed magnetoplasmadynamic thruster and the bismuth-fed Hall thruster. An important element of part of the maturation of these thrusters will be the development of compact, reliable conductive-propellant feed system components. In the present paper we provide design considerations and experimental calibration data for electromagnetic (EM) pumps. The role of an electromagnetic pump in a liquid metal feed system is to establish a pressure gradient between the propellant reservoir and the thruster - to establish the requisite mass flow rate. While EM pumps have previously been used to a limited extent in nuclear reactor cooling loops, they have never been implemented in electric propulsion (EP) systems. The potential benefit of using EM pumps for EP are reliability (no moving parts) and the ability to precisely meter the propellant flow rate. We have constructed and tested EM pumps that use gallium, lithium, and bismuth propellants. Design details, test results (pressure developed versus current), and material compatibility issues are reported. It is concluded that EM pumps are a viable technology for application in both laboratory and flight EP conductive-propellant feed systems.

Markusic, T. E.↗
NASA NTRS1990

A conflict analysis of 4D descent strategies in a metered, multiple-arrival route environment

A conflict analysis was performed on multiple arrival traffic at a typical metered airport. The Flow Management Evaluation Model (FMEM) was used to simulate arrival operations using Denver Stapleton's arrival route structure. Sensitivities of conflict performance to three different 4-D descent strategies (clear-idle Mach/Constant AirSpeed (CAS), constant descent angle Mach/CAS and energy optimal) were examined for three traffic mixes represented by those found at Denver Stapleton, John F. Kennedy and typical en route metering (ERM) airports. The Monte Carlo technique was used to generate simulation entry point times. Analysis results indicate that the clean-idle descent strategy offers the best compromise in overall performance. Performance measures primarily include susceptibility to conflict and conflict severity. Fuel usage performance is extrapolated from previous descent strategy studies.

Izumi, K. H.↗
NASA NTRS2015

A Sinuous Tumulus over an Active Lava Tube at Klauea Volcano: Evolution, Analogs, and Hazard Forecasts

Inflation of narrow tube-fed basaltic lava flows (tens of meters across), such as those confined by topography, can be focused predominantly along the roof of a lava tube. This can lead to the development of an unusually long tumulus, its shape matching the sinuosity of the underlying lava tube. Such a situation occurred during Klauea Volcanos (Hawaii, USA) ongoing East Rift Zone eruption on a lava tube active from July through November 2010. Short-lived breakouts from the tube buried the flanks of the sinuous, ridge-like tumulus, while the tumulus crest, its surface composed of lava formed very early in the flows emplacement history, remained poised above the surrounding younger flows. At least several of these breakouts resulted in irrecoverable uplift of the tube roof. Confined sections of the prehistoric Carrizozo and McCartys flows (New Mexico, USA) display similar sinuous, ridge-like features with comparable surface age relationships. We contend that these distinct features formed in a fashion equivalent to that of the sinuous tumulus that formed at Kīlauea in 2010. Moreover, these sinuous tumuli may be analogs for some sinuous ridges evident in orbital images of the Tharsis volcanic province on Mars. The short-lived breakouts from the sinuous tumulus at Kīlauea were caused by surges in discharge through the lava tube, in response to cycles of deflation and inflation (DI events) at Kīlauea's summit. The correlation between DI events and subsequent breakouts aided in lava flow forecasting. Breakouts from the sinuous tumulus advanced repeatedly toward the sparsely populated Kalapana Gardens subdivision, destroying two homes and threatening others. Hazard assessments, including flow occurrence and advance forecasts, were relayed regularly to the Hawaiʻi County Civil Defense to aid their lava flow hazard mitigation efforts while this lava tube was active.

volcanic hazards↗
NASA NTRS1973

Laser velocimeter for simultaneous two-dimensional velocity measurements

Laser velocimeter provides simultaneous orthogonal measurements in manner which minimizes many problems attending prior systems, and allows spatial traversing of flowfield in order to obtain velocity profiles. Velocimeter permits rapid interrogation of unsteady flows where area of interest is of the order of one meter in extent and flow does not vary appreciably over time of about one second.

Orloff, K. L.↗
NASA NTRS1984

NASA/MSFC ground-based Doppler lidar nocturnal boundary layer experiment (Noblex)

During the summer of 1982, NASA/MSFC's ground-based CO2 Doppler Lidar Velocimeter (DLV) was deployed at the Denver Stapleton Airport as part of NASA's participation in the JAWS (Joint Airport Weather Studies) program. Configured to measure the radial wind component within a 10 km radius, the conically scanning lidar was used to examine the evolution of a nocturnal boundary layer under the conditions of cloud free skies and rolling terrain. A valley drainage flow was detected and a two dimension flow visualization constructed. The depth of the gravity current was -700 meters while the depth of the creek valley was -150 meters. This deep drainage flow was detectable for distances of 30 to 40 km from the exit region of the valley. Although the sample period (2000 to 2300 CST) was short and only one nocturnal boundary layer case examined, the usefulness of the DLV was demonstrated as well as the care that must be exercised in interpreting lidar data taken in a stable boundary layer in the vicinity of subtle terrain features.

Emmitt, G. D.↗