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At least 253 records · Page 14

Hydrological control of chemical weathering and rock-carbon fluxes: East River, Colorado

This data package is used in the manuscript entitled “Hydrological control of chemical weathering and rock-carbon fluxes”. The field study was conducted in a lower montane hillslope of the East River watershed, underlain by Mancos Shale, within the lower 140 m section of a transect that extends nearly 1 km to its local peak. The data in this package, in CSV file format, were collected from Fall 2016 to fall 2021, including depth-resolved dynamic water table depths, subsurface water fluxes, solid phase (soil to bedrock) chemical compositions, geochemical properties of porewater and pore-gas, including radio carbon concentrations. The detailed methods of field studies, laboratory chemical analyses, and calculations are described in the Methods section and in the dataset file: Wan_et_al_Methods.pdf. The dataset additionally includes a file-level metadata (flmd.csv) file that lists each file contained in the dataset with associated metadata; and a data dictionary (dd.csv) file that contains column/row headers used throughout the files along with a definition, units, and data type.

54 ENVIRONMENTAL SCIENCES↗

Multi‐season analysis reveals hundreds of drought‐responsive genes in sorghum

Persistent drought affects global crop production and is becoming more severe in many parts of the world in recent decades. Deciphering how plants respond to drought will facilitate the development of flexible mitigation strategies. Sorghum bicolor L. Moench (sorghum), a major cereal crop and an emerging bioenergy crop, exhibits remarkable resilience to drought. To better understand the molecular traits that underlie sorghum's remarkable drought tolerance, we undertook a large-scale sorghum gene expression profiling effort, totaling nearly 1500 transcriptome profiles, across a 3-year field study with replicated plots in California's Central Valley. This study included time-resolved gene expression data from roots and leaves of two sorghum genotypes, BTx642 and RTx430, with different pre-flowering and post-flowering drought-tolerance adaptations under control and drought conditions. Quantification of genotype-specific drought tolerance effects was enabled by de novo sequencing, assembly, and annotation of both BTx642 and RTx430 genomes. These reference-quality genomes were used to construct a pangene set for characterizing conserved and genotype-specific expression. By integrating time-resolved transcriptomic responses to drought in the field across three consecutive years, we identified a set of 726 drought-responsive genes that responded similarly in all 3 years of our field study. Functional enrichment analysis identified abiotic stress, secondary cell wall-related processes and metabolism as particularly affected under both types of drought stress. We also found that some glyoxylate cycle pathway genes, including malate synthase and isocitrate lyase, are differentially regulated particularly during post-flowering drought stress, implicating this pathway as potentially important for drought responsiveness. This expansive dataset represents a unique resource for sorghum and drought research communities and provides a methodological framework for the integration of multi-faceted time-resolved transcriptomic datasets.

Cole, Benjamin [USDOE Joint Genome Institute (JGI)↗

Highly Variable Cycle Exhaust Model Test (HVC10)

Results from acoustic and flow-field studies using the Highly Variable Cycle Exhaust (HVC) model were presented. The model consisted of a lobed mixer on the core stream, an elliptic nozzle on the fan stream, and an ejector. For baseline comparisons, the fan nozzle was replaced with a round nozzle and the ejector doors were removed from the model. Acoustic studies showed far-field noise levels were higher for the HVC model with the ejector than for the baseline configuration. Results from Particle Image Velocimetry (PIV) studies indicated that large flow separation regions occurred along the ejector doors, thus restricting flow through the ejector. Phased array measurements showed noise sources located near the ejector doors for operating conditions where tones were present in the acoustic spectra.

Henderson, Brenda↗

Using Wind and Temperature Fields to Study Dehydration Mechanisms in the Tropical Tropopause Layer

The tropics are the main region for troposphere-to-stratosphere transport (TST) of air. One of the dominant mechanisms that control tropical TST of water vapor is freeze-drying by the cold tropical tropopause. This mechanism is supported by evidence from satellite observations of the "tape recorder", where seasonal changes in stratospheric water vapor are in phase with seasonal changes in tropopause temperatures in the tropics. Over the last few years, however, the concept of the tropical tropopause has evolved from a single material surface to a layer called the Tropical Tropopause Layer (TTL). A recent hypothesis on dehydration mechanisms suggests that dehydration and entry point into the stratosphere are not always co-located (Holton and Gettelman, 2001). Instead, dehydration can occur during horizontal advection through Lagrangian 'cold pools', or coldest regions along a parcel's trajectory, as air ascends within the TTL while the entry point into the stratosphere occurs at a different geographical location. In this study, we investigate the impact that these Lagrangian cold pools have on TTL moisture. For this purpose, we use in situ measurements of TTL water vapor obtained aboard NASA's WB-57 aircraft over the Eastern Tropical Pacific, and we compare these measurements to minimum saturation water vapor mixing ratios obtained from three-dimensional backward trajectory calculations. Aircraft measurements show frequent unsaturated conditions, which suggest that the entry value of stratospheric water vapor in this region was not set by local saturation conditions. Trajectory calculations, driven by both ECMWF operational analysis and reanalysis winds and temperature fields, are used to explore the impact (e.g., geographical location, timing, dehydration magnitude) of the Lagrangian cold pools intercepted by the parcels sampled by the aircraft. We find noteworthy differences in the location of the Lagrangian cold pools using the two ECMWF data sets, namely influence of the Western Tropical Pacific region when using operational analysis fields versus influence of the Eastern Tropical Pacific and South America regions when using reanalysis fields. These results have a significant impact on our scientific conclusions on dehydration mechanisms affecting the air sampled by the aircraft, given that these regions have different thermodynamic and convective properties.

Pittman, Jasna↗

Additional Metadata Guidelines to Improve the Structure and Usability of HDF and NetCDF Files

The Hierarchical Data Format (HDF) and Network Common Data Form (NetCDF) are data file formats created to aid users in the creation or use of scientific data. These file formats are useful for handling large data volumes and hosting extensive metadata as global attributes or variables and are popular with the modeling community. HDF and NetCDF files are largely used with remote sensing data and have been used to support measurements from numerous campaigns, from satellite to aircraft or ground and mobile based measurements. The files from airborne field studies, however, vary greatly in terms of the file structure and the amount and content of metadata. Information relevant to the file that can be useful to the user such as the data producer, location where data was taken, variable descriptions, or information about the instrument might not be included in the file. This metadata might be present in another file in the dataset containing the same data using the International Consortium for Atmospheric Research on Transport and Transformation (ICARTT) format. Recently, the Aerosols, Clouds, and their Interactions for Earth System Models (MACIE) group started a grassroots effort to develop a set of requirements for the HDF and NetCDF files for field studies, aiming to make the data products more interoperable and usable. Particularly, these requirements seek to make the files more compliant to Climate and Forecast (CF) metadata conventions and to standardize the file structure and the global and variable attributes. These requirements would help to ensure that HDF and NetCDF files contain adequate metadata to better support their use for research, e.g., the modeling community, and to enhance the usability and interoperability of data for research communities at large. To be presented are the details of the MACIE requirements as well as examples of the implementation of these requirements for merge files and lidar observation data files.

Sean Leavor↗

Application of remote sensing for prediction and detection of thermal pollution, phase 2

The development of a predictive mathematical model for thermal pollution in connection with remote sensing measurements was continued. A rigid-lid model has been developed and its application to far-field study has been completed. The velocity and temperature fields have been computed for different atmospheric conditions and for different boundary currents produced by tidal effects. In connection with the theoretical work, six experimental studies of the two sites in question (Biscayne Bay site and Hutchinson Island site) have been carried out. The temperature fields obtained during the tests at the Biscayne Bay site have been compared with the predictions of the rigid-lid model and these results are encouraging. The rigid-lid model is also being applied to near-field study. Preliminary results for a simple case have been obtained and execution of more realistic cases has been initiated. The development of a free-surface model also been initiated. The governing equations have been formulated and the computer programs have been written.

Veziroglu, T. N.↗

Multiscale Data-Driven Seismic Full-Waveform Inversion With Field Data Study

Seismic full-waveform inversion (FWI), which uses iterative methods to estimate high-resolution subsurface models from seismograms, is a powerful imaging technique in exploration geophysics. In recent years, the computational cost of FWI has grown exponentially due to the increasing size and resolution of seismic data. Moreover, it is a nonconvex problem and can encounter local minima due to the limited accuracy of the initial velocity models or the absence of low frequencies in the measurements. To overcome these computational issues, we develop a multiscale data-driven FWI method based on fully convolutional networks (FCNs). In preparing the training data, we first develop a real-time style transform method to create a large set of synthetic subsurface velocity models from natural images. We then develop two convolutional neural networks with encoder-decoder structures to reconstruct the low- and high-frequency components of the subsurface velocity models, separately. To validate the performance of our data-driven inversion method and the effectiveness of the synthesized training set, we compare it with conventional physics-based waveform inversion approaches using both synthetic and field data. Finally, these numerical results demonstrate that, once our model is fully trained, it can significantly reduce the computation time and yield more accurate subsurface velocity models in comparison with conventional FWI.

58 GEOSCIENCES↗

Hydropower Biological Evaluation Toolset Best Practice Guide

Field studies using live fish are necessary for the evaluation of turbine biological performance, but they cannot determine the specific hydraulic conditions or physical stresses experienced by the fish, the locations where deleterious conditions occur, or the specific causes of the biological response. Using the Sensor Fish (SF) sensing technology, this deficiency can be overcome because the SF can be released independently or concurrently with live fish directly into operating infrastructure, and it takes high-frequency measurements of hydraulic conditions such as pressure, acceleration, and rotation acting on a body in situ during downstream passage. The Hydropower Biological Evaluation Tools (HBET; Hou et al. 2018) software package, developed by Pacific Northwest National Laboratory (PNNL), is designed to assemble, organize, and process data collected by the PNNL-developed SF and by live fish. HBET was developed specifically to design SF field studies, process the raw data, and analyze the processed data efficiently and scientifically. Its objectives are to facilitate SF studies focused on characterizing hydraulic conditions and to apply SF data for evaluating the impacts on fish from passage through hydro-structures. HBET allows users to design new studies, analyze data, perform statistical analyses, and evaluate predicted biological responses. It can be used by researchers, turbine designers, hydropower operators, and regulators to evaluate hydro-structures to enhance environmental sustainability in a cost-effective manner.

13 HYDRO ENERGY↗

Evaluation of survival estimates generated from tracking downstream migrating juvenile sea lamprey ( Petromyzon marinus ) with a miniature acoustic telemetry tag

Fish that undertake juvenile migration pass through habitats that vary in mortality risk. The ability to identify regions of persistently low survival would improve fishery management. We conducted a field study combined with predictive modeling of survival in downstream migrating juvenile sea lamprey using a new micro-acoustic telemetry tag designed for implantation into small, slender-bodied fishes. Detection data were collected from eight receivers placed across a coastal riverine–wetland–lake complex. Juvenile sea lamprey initiated downstream movement near nautical twilight, stopped frequently, and were more likely to move during high river discharge. Estimated survival was highest in the riverine reach, declined through the river–wetland complex, and dropped precipitously in the drowned rivermouth lake. However, the high transmission rate and resulting short battery life of the microtransmitters (as configured) likely resulted in missed detections in the lower reaches. Simulation analyses suggested survival estimation could be improved by increasing the number of tagged lamprey and staggering release locations. We offer practical recommendations for the use of this new transmitter in field studies with small anguilliform fish.

59 BASIC BIOLOGICAL SCIENCES↗

Isotropic seismic radiation from rock damage and dilatancy

SUMMARY We present semi-analytical and numerical results on isotropic radiation expected to be generated by seismic sources with damaged dilatant materials that sustain during failure an overall shear deformation. The results are derived for inclusions with different stiffness components, including those responsible for shear-volumetric coupling in dilatant material, and different geometries, in a surrounding elastic solid. The obtained relations indicate that the isotropic seismic potency and seismic moment components are proportional to the shear potency components multiplied by the aspect ratio between the thickness and length of the source zone. For a typical rupture zone with a thin tabular geometry, the expected small aspect ratio explains the general seismological observations of small isotropic radiation. However, isotropic radiation induced by dynamic dilation for short and thick sources is more pronounced and may reach for spherical sources sustaining shear deformation 18 per cent of the shear components. Sources that include also tensile components and explosions can produce larger isotropic radiation. The results provide rigorous relations that can be used to estimate different source properties in numerical simulations, experiments and field studies.

Geochemistry & Geophysics↗

Mechanisms of Waterflood Inefficiency: Analysis of Geological, Petrophysical and Reservoir History, a Field Case Study of FWU (East Section)

The petroleum reservoir represents a complex heterogeneous system that requires thorough characterization prior to the implementation of any incremental recovery technique. One of the most commonly utilized and successful secondary recovery techniques is waterflooding. However, a lack of sufficient investigation into the inherent behavior and characteristics of the reservoir formation in situ can result in failure or suboptimal performance of waterflood operations. Therefore, a comprehensive understanding of the geological history, static and dynamic reservoir characteristics, and petrophysical data is essential for analyzing the mechanisms and causes of waterflood inefficiency and failure. In this study, waterflood inefficiency was observed in the Morrow B reservoir located in the Farnsworth Unit, situated in the northwestern shelf of the Anadarko Basin, Texas. To assess the potential mechanisms behind the inefficiency of waterflooding in the east half, geological, petrophysical, and reservoir engineering data, along with historical information, were integrated, reviewed, and analyzed. The integration and analysis of these datasets revealed that several factors contributed to the waterflood inefficiency. Firstly, the presence of abundant dispersed authigenic clays within the reservoir, worsened by low reservoir quality and high heterogeneity, led to unfavorable conditions for waterflood operations. The use of freshwater for flooding exacerbated the adverse effects of sensitive and migratory clays, further hampering the effectiveness of the waterflood. In addition to these factors, several reservoir engineering issues played a significant role in the inefficiency of waterflooding. These issues included inadequate perforation strategies due to the absence of detailed hydraulic flow units (HFUs) and rock typing, random placement of injectors, and uncontrolled injected fresh water. These external controlling parameters further contributed to the overall inefficiencies observed during waterflood operations in the east half of the reservoir. A detailed understanding of the mechanistic factors of inefficient waterflood operation will provide adequate insights into the development of the improved recovery technique for the field.

Morgan, Anthony (ORCID:0000000211519153)↗

Nitrogen derived from Combined Algal Processing supports algae cultivation for biofuels

Algae biomass, a biofuel feedstock with potential to reduce global CO 2 emissions, is currently too expensive Nitrogen, a significant input and life cycle cost, requires research on recycling and additional sources to reduce costs. Integral to nitrogen recycling is the knowledge of actual nitrogen requirements in outdoor ponds, thus data from the Unified Field Studies of the Algae Testbed Public-Private Partnership was analyzed. The data showed that Nannochloropsis oceanica used 14–24 mg/L NH 4 + per harvest cycle for robust growth. Nitrogen recycled directly from the algae conversion process will likely be the primary source of nitrogen for algae cultivation. Harvested algae can be fractionated via Combined Algal Processing (CAP), where algae are pretreated, fermented, and the fuel feedstocks (e. g. ethanol, lipids) are removed leaving a nitrogen rich slurry. Chlorella vulgaris grew robustly in nitrogen-free media supplemented with CAP residues through 4 harvest cycles with no inhibition. The amount of nitrogen as free ammonium available for recycling directly without further processing was insufficient (~4% of total needed), thus the nitrogen fixing bacteria, Azotobacter vinelandii, was investigated as a means for increasing ammonium concentration in CAP residues. This organism was shown to be proficient at growth on sugars liberated from algae biomass during pretreatment and sustained algae growth using A. vinelandii supernatants was also demonstrated. Using Azotobacter to increase ammonium concentrations on-site using an inexpensive biological process to generate the necessary ammonium for outdoor algae cultivation improves sustainability of algae biofuels by avoiding the energy intensive steps of typical ammonium production and transport. Furthermore, these results show that byproducts of algae conversion begin to close nitrogen demand and, with further supplementation by diazotrophic bacteria or protein hydrolysis, may completely replace current energyintensive and costly industrial nitrogen sources offering a path to increased economic feasibility and improved life cycle analysis of algae-based biofuels.

09 BIOMASS FUELS↗

Novel and Emerging Capabilities that Can Provide a Holistic Understanding of the Plant Root Microbiome

In recent years, the root microbiome (i.e., microorganisms growing inside, on, or in close proximity to plant roots) has been shown to play an important role in plant health and productivity. Despite its importance, the root microbiome is challenging to study because of its complexity, heterogeneity, and subterranean location. Fortunately, root microbiome research has seen a tremendous influx of novel technologies (e.g., imaging tools, robotics, and molecular analyses), experimental platforms (e.g., micro- and mesocosms), and data integration, modeling, and prediction tools in the past decade that have greatly increased our ability to dissect the complex network of interactions between above- and belowground environmental parameters, plants, bacteria, and fungi that dictate soil and broader ecosystem health. Herein, we discuss methods that are currently used in root microbiome research and that can be expanded to phytobiome research in general ranging from laboratory studies to mesocosm-scale studies and, finally, to field studies; evaluate their relevance to ecosystem studies; and discuss future root microbiome research directions.

59 BASIC BIOLOGICAL SCIENCES↗

Main Bearing Replacement and Damage - A Field Data Study on 15 Gigawatts of Wind Energy Capacity

This study seeks to establish a comprehensive baseline of knowledge for the replacement and damage of main bearings in wind turbines. The purpose of this report is to provide a high-level summary of the data set, methodology, and results of this work. Full technical details and an extended analysis will be made available in a future publication. We collected data on main bearing replacements and reported damage from industrial partners based in Europe and the United States. In total, we obtained data for 167 wind power plants, with a combined capacity of 15.3 gigawatts (GW). Most of the data set was comprised of land-based, three-point mount, spherical roller bearings. Within this data set were 689 instances of main bearing replacement. Analysis was undertaken in two parts: first, a statistical analysis of the main bearing time-to-replacement data using survival analysis techniques; second, quantitative and qualitative analyses of the obtained damage information. Our results showed that 10% of a fixed main bearing population would be expected to have been replaced by 10.5 years. This is close to half of the 20-year design value. Fitted parametric distributions then indicated that by year 20, between 22% and 25% of main bearings are expected to have been replaced. Analysis of the damage reports revealed spalling to be the main type of damage listed. The additional presence of surface damage in the collected data indicates that at least part of the spalling cases are likely due to surface-initiated rolling contact fatigue. At this stage is not clear what proportion of spalling cases result from "wear induced", surface-initiated and subsurface-initiated rolling contact fatigue. While this work provides important insights into the current state of main bearing replacements and damage, many questions remain. An ongoing and expanding data collection and analysis effort focused on wind turbine main bearings is therefore recommended.

17 WIND ENERGY↗

Development of Operando Atom Probe to study field-assisted surface chemistry

Recent efforts at PNNL have led to the development of in-situ Atom Probe Tomography (ISAPT) and Operando Atom Probe (OAP), establishing PNNL as a key player in this technology, both in the U.S. and globally. OAP has already pushed the initial limits of APT, which was traditionally used at cryo temperatures (<60 K) and ultra-high vacuum (<10⁻¹² bar), to operate in the presence of reactive gases (up to 10⁻¹⁰ bar) at a maximum temperature of 300 K. Using OAP, we successfully observed Co and Fe oxidation in real-time at the nanoscale, revealing the importance of surface structures in these interactions. Additionally, OAP opens the possibility to explore the influence of local electric fields (EFs) on surface chemistry and hydrogen interactions at the nanoscale.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗