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At least 253 records · Page 14

Constant-Distance Mode Nanospray Desorption Electrospray Ionization Mass Spectrometry Imaging of Biological Samples with Complex Topography

A new approach for constant distance mode mass spectrometry imaging of biological samples using nanospray desorption electrospray ionization (nano-DESI MSI) was developed by integrating a shear-force probe with nano-DESI probe. The technical concept and basic instrumental setup as well as general operation of the system are described. Mechanical dampening of resonant oscillations due to the presence of shear forces between the probe and the sample surface enables constant-distance imaging mode via a computer controlled closed feedback loop. The capability of simultaneous chemical and topographic imaging of complex biological samples is demonstrated using living Bacillus Subtilis ATCC 49760 colonies on agar plates. The constant-distance mode nano-DESI MSI enabled imaging of many metabolites including non-ribosomal peptides (surfactin, plipastatin and iturin) and iron-bound heme on the surface of living bacterial colonies ranging in diameter from 10 mm to 13 mm with height variations of up to 0.8 mm above the agar plate. Co-registration of ion images to topographic images provided higher-contrast images. Constant-mode nano-DESI MSI is ideally suited for imaging biological samples of complex topography in their native state.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

Seismic Imaging of a Shale Landscape Under Compression Shows Limited Influence of Topography–Induced Fracturing

We used seismic refraction to image the P-wave velocity structure of a shale watershed experiencing regional compression in the Valley and Ridge Province (USA). From estimates showing strong compressional stress, we expected the depth to unweathered bedrock to mirror the hill-valley-hill topography (“bowtie pattern”) by analogy to seismic velocity patterns in crystalline bedrock in the North American Piedmont that also experience compression. Previous researchers used failure potentials calculated for strong compression in the Piedmont to suggest fractures are open deeper under hills than valleys to explain the “bowtie” pattern. Seismic images of the shale watershed, however, show little evidence of such a “bowtie.” Instead, they are consistent with weak (not strong) compression. Here, this contradiction could be explained by the greater importance of infiltration-driven weathering than fracturing in determining seismic velocities in shale compared to crystalline bedrock, or to local perturbations of the regional stress field due to lithology or structures.

58 GEOSCIENCES↗

X-ray topography using the forward transmitted beam under multiple-beam diffraction conditions

X-ray topographs are taken for a sapphire wafer with the [0001] surface normal, as an example, by forward transmitted synchrotron x-ray beams combined with two-dimensional electronic arrays in the x-ray detector having a spatial resolution of 1 μm. They exhibit no shape deformation and no position shift of the dislocation lines on the topographs. Since the topography is performed under multiple-beam diffraction conditions, the topographic images of a single diffraction (two-wave approximation condition) or plural diffractions (six-wave approximation condition) can be recorded without large specimen position changes. As usual Lang topographs, it is possible to determine the Burgers vector of each dislocation line. Because of high parallelism of the incoming x-rays and linear sensitivity of the electronic arrays to the incident x-rays, the present technique can be used to visualize individual dislocations in single crystals of the dislocation density as high as 1 × 10{sup 5} cm{sup −2}.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗

Growth and surface topography of WSe{sub 2} single crystal

Tungsten Di-Selenide belongs to the family of TMDCs showing their potential applications in the fields of Optoelectronics and PEC solar cells. Here in the present investigation single crystals of WSe{sub 2} were grown by Direct Vapour Transport Technique in a dual zone furnace having temperature difference of 50 K between the two zones. These single crystals were characterized by EDAX which confirms the stiochiometry of the grown crystals. Surface topography of the crystal was studied by optical micrograph showing the left handed spirals on the surface of WSe{sub 2} crystals. Single crystalline nature of the crystals was confirmed by SAED.

75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND↗

The effect of surface topography on the micellisation of hexadecyltrimethylammonium chloride at the silicon-aqueous interface

Amphiphilic aggregation at solid-liquid interfaces can generate mesostructured micelles that can serve as soft templates. In this study we have simulated the self-assembly of hexadecyltrimethylammonium chloride (C16TAC) surfactants at the Si(100)- and Si(111)-aqueous interfaces. The surfactants are found to form semicylindrical micelles on Si(100) but hemispherical micelles on Si(111). This difference in micelle structure is shown to be a consequence of the starkly different surface topographies that result from the reconstruction of the two silicon surfaces. This reveals that micelle structure can be governed by epitaxial matching even with non-polar substrates.

CTAC↗

Surface Topography and Optical Properties of Thin AlN Films Produced on GaAs (100) Substrate by Reactive Ion-Plasma Sputtering

A study of the surface topography and optical characteristics of thin AlN films used as passivating and antireflection coatings deposited on n-GaAs (100) substrates by reactive ion-plasma sputtering is reported. It was found that the process conditions affect the structure and the optical characteristics of the films, which makes it possible to obtain coatings with prescribed parameters. An analysis of the results furnished by ellipsometry and atomic-force microscopy of the surface shows that the refractive index of the films is correlated with the surface structure.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗

Remote Sensing and GIS data at 1km-grid over Chesapeake Bay used in “He et al. 2024, Effects of spatial variability in vegetation phenology, climate, landcover, biodiversity, topography, and soil property on soil respiration across a coastal ecosystem”

The package contains the data layers used in “He et al. 2024, Effects of spatial variability in vegetation phenology, climate, landcover, biodiversity, topography, and soil property on soil respiration across a coastal ecosystem”. The study aims to use multi-source remote sensing and GIS datasets to investigate the spatial heterogeneity and identify spatial zones with similar environmental characteristics and understand the primary driving factors affecting soil respiration within sub-ecosystems of the coastal ecosystem. We employed unsupervised hierarchical clustering analysis to identify spatial regions with distinct environmental characteristics, then determined the main driving factors using Random Forest regression and SHapley Additive exPlanations (SHAP). Spatial data layers include soil respiration, kernel Normalized Difference Vegetation Index (kNDVI) computed from Harmonized Landsat 8 and Sentinel-2 time series, climate variables from the Daymet dataset, land cover, biodiversity, topographical metrics, soil property, and tidal elevation.

54 ENVIRONMENTAL SCIENCES↗

(abstract) The Shuttle Radar Topography Mapper

The Shuttle Radar Topography Mapper (SRTM), is a cooperative project between NASA and the Defense Mapping Agency of the U.S. Department of Defense. The mission is designed to use a single-pass radar interferometer to produce a digital elevation model of the Earth's land surface between about 60 degrees north and south latitude. The DEM will have 30 m horizontal resolution and about 10 m vertical errors.

Shuttle Radar Topography Mapper interferometry glo↗

The wide swath ocean altimeter: algorithm and technology developments for improved ocean topography measurements

The Wide Swath Ocean Altimeter (WSOA) is a recently proposed interferometric instrument that would provide nearly complete global ocean topography measurements from a single platform. Several new algorithm and technology developments improve the expected WSOA performance, and facilitate the feasibility of including WSOA on a next generation altimeter mission. Those developments are discussed in this paper.

interferometry↗

The Glacier and Land Ice Surface Topography Interferometer (GLISTIN): A Novel Ka-band Digitally Beamformed Interferometer

The estimation of the mass balance of ice sheets and glaciers on Earth is a problem of considerable scientific and societal importance. A key measurement to understanding, monitoring and forecasting these changes is ice-surface topography, both for ice-sheet and glacial regions. As such NASA identified 'ice topographic mapping instruments capable of providing precise elevation and detailed imagery data for measurements on glacial scales for detailed monitoring of ice sheet, and glacier changes' as a science priority for the most recent Instrument Incubator Program (IIP) opportunities. Funded under this opportunity is the technological development for a Ka-Band (35GHz) single-pass digitally beamformed interferometric synthetic aperture radar (InSAR). Unique to this concept is the ability to map a significant swath impervious of cloud cover with measurement accuracies comparable to laser altimeters but with variable resolution as appropriate to the differing scales-of-interest over ice-sheets and glaciers.

ice surface topography↗