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NASA NTRS2000

Optical Variability of BL Lacertae During the Major Outburst of 1997

We have undertaken an investigation of recent flux variability in BL Lac. We present optical observations taken over 22 nights documenting major as well as minor outbursts. This has been combined, for purposes of multifrequency analysis, with published X-ray and T-ray data taken for an additional single night, On two nights in particular, including the night of the X-ray observations, a major outburst of about a full magnitude of variation was recorded. All the data have been analyzed with theoretical models. Attempts were made to use synchrotron self-Compton and external Comptonization models to explain the data; however, both classes of models were found lacking. More satisfactory results were obtained using an analytical model proposed by Wang et al. that involves the evolution of synchrotron spectra in a homogeneous jet due to the injection of relativistic electrons, taking into account radiation losses during the outbursts. It is hoped that the results of this study of BL Lac, an archetype for the class of blazars in general, represent a more generic phenomenon applicable to the entire class.

Ghosh, K. K.↗

NASA NTRS2004

Human cutaneous vascular responses to whole-body tilting, Gz centrifugation, and LBNP

We hypothesized that gravitational stimuli elicit cardiovascular responses in the following order with gravitational stress equalized at the level of the feet, from lowest to highest response: short-(SAC) and long-arm centrifugation (LAC), tilt, and lower body negative pressure (LBNP). Up to 15 healthy subjects underwent graded application of the four stimuli. Laser-Doppler flowmetry measured regional skin blood flow. At 0.6 G(z) (60 mmHg LBNP), tilt and LBNP similarly reduced leg skin blood flow to approximately 36% of supine baseline levels. Flow increased back toward baseline levels at 80-100 mmHg LBNP yet remained stable during 0.8-1.0 G(z) tilt. Centrifugation usually produced less leg vasoconstriction than tilt or LBNP. Surprisingly, SAC and LAC did not differ significantly. Thigh responses were less definitive than leg responses. No gravitational vasoconstriction occurred in the neck. All conditions except SAC increased heart rate, according to our hypothesized order. LBNP may be a more effective and practical means of simulating cardiovascular effects of gravity than centrifugation.

Motor Activity/physiology↗

NASA NTRS2005

Coronal Dynamics and Complete Flare Energy Budget for the M Dwarf AD Leo

This grant supported the observing and data analysis for FUSE Cycle 3 project C114 to observe the flare star AD Leo for 50 ksec coordinated with HST (Hubble Space Telescope) STIS ultraviolet spectroscopy and Chandra X-ray spectroscopy. Unfortunately, it was impossible to obtain the planned FUSE observations because AD Leo is a low declination target (delta approximately 20 degrees) and was rendered unobservable by restrictions in the sky coverage for FUSE observations. In April 2002 another M dwarf star, EV Lac, which is at significantly higher declination, was substituted for this project. EV Lac was observed by FUSE for a cumulative exposure of 35 ksec on 2002 July 1. The observation used the large LWRS aperture and collected data in time-tagged mode. The LWRS aperture is large enough that the target should remain within the aperture despite the normal level of pointing jitter and target drift experienced during FUSE observing. Our examination of the stellar signal showed that the target was well within the aperture throughout the observation. The data were split into night-time and day-time data so that the effects of airglow emission were recognizable. No obvious flaring, the primary science objective, was detected during the observation. The only stellar lines detected are 0 VI 1031.9, 1037.6 Angstrom, and the C III 1175 Angstrom, UV multiplet and the 977 Angstrom, resonance line. A comparison of the day-time and night-time spectra show that the 0 VI lines and the C III intersystem multiplet are unaffected by airglow features. The day-time data 977 Angstrom profile shows the presence of significant scattered solar C III photons, which should not be present in the night time spectrum. Emission fluxes for these lines were measured by direct summation of the emission lines. No continuum signal was detected in the region of these lines. The cleanest emission line profiles are for the O VI lines and we performed Gaussian fitting for these profiles. Both lines are clearly skewed with the emission peak shifted shortward of the line centroid and with emission extending further in the longward wing than for the shortward wing. A single Gaussian fits the 1032 Angstrom line profile reasonably and gives a line flux similar to that from direct integration with a line width (FWHM) of 0.16 Angstrom, (46 kilometers per second).

Brown, Alexander↗

NASA NTRS2007

Does the Blazar Gamma-ray Spectrum Harden with Increasing Flux? - Analysis of Nine Years of EGRET Data

The Energetic Gamma Ray Experiment Telescope (EGRET) on the Compton Gamma Ray Observatory (CGRO) discovered gamma-ray emission from more than 67 blazars during its nine-year lifetime. We conducted an exhaustive search of the EGRET archives and selected all the blazars that were observed multiple times and were bright enough to enable a spectral analysis using standard powerlaw models. The sample consists of 18 flat-spectrum radio quasars (FSRQs), 6 low-frequency-peaked BL Lacs (LBLs) and 2 high-frequency-peaked BL Lacs (HBLs). We do not detect any clear pattern in'the variation of spectral index with flux. Some of the blazars do not show any statistical evidence for spectral variability. The spectrum hardens with increasing flux in a few cases. There is also evidence for a flux-hardness anticorrelation at lo\v fluxes in five blazars. The well observed blazars (3C 279,3C 273, PKS 0528-i-134, PKS 1622-297, PKS 0208- 512) do not show any overall trend in the long-term spectral dependence on flux, but the sample shows a mixture of hard and soft states. We observed spectral hysteresis at weekly timescales in all the three FSRQs for which data from flares lasting for 3 approx. 4 weeks were available. All three sources show a counterclockwise rotation despite the widely different flux profiles. Hysteresis in the spectral index vs. flux space has never been observed in FSRQs in gamma-rays at weekly timescales. itre analyze the observed spectral behavior in the context of various inverse-Compton mechanisms believed to be responsible for emission in the EGRET energy range. Our analysis uses the EGRET skymaps that were regenerated to include the changes in performance during the mission.

Nandikotkur, Giridhar↗

NASA NTRS2014

The Unusual Multiwavelength Properties of the Gamma-Ray Source PMN J1603-4904

Context. We investigate the nature and classification of PMNJ1603-4904, a bright radio source close to the Galactic plane, which is associated with one of the brightest hard-spectrum gamma-ray sources detected by Fermi/LAT. It has previously been classified as a low peaked BL Lac object based on its broadband emission and the absence of optical emission lines. Optical measurements, however, suffer strongly from extinction and the absence of pronounced short-time gamma-ray variability over years of monitoring is unusual for a blazar. Aims. In this paper, we are combining new and archival multi-wavelength data of PMNJ1603-4904 in order to reconsider the classification and nature of this unusual gamma-ray source. Methods. For the first time, we study the radio morphology of PMNJ1603-4904 at 8.4GHz and 22.3GHz, and its spectral properties on milliarcsecond scales, based on VLBI observations from the TANAMI program. We combine the resulting images with multi-wavelength data in the radio, IR, optical/UV, X-ray, and gamma-ray regimes. Results. PMNJ1603-4904 shows a symmetric brightness distribution at 8.4 GHz on milliarcsecond scales, with the brightest, and most compact component in the center of the emission region. The morphology is reminiscent of a compact symmetric object (CSO). Such objects, thought to be young radio galaxies, have been predicted to produce gamma-ray emission but have not been detected as a class by the Fermi gamma-ray telescope so far. Sparse (u, v)-coverage at 22.3GHz prevents an unambiguous modeling of the source morphology at this higher frequency. Moreover, infrared measurements reveal an excess in the spectral energy distribution (SED), which can be modeled with a blackbody with a temperature of about 1600 K, and which is usually not present in blazar SEDs. Conclusions. The TANAMI VLBI data and the shape of the broadband SED challenge the current blazar classification of one of the brightest gamma-ray sources in the sky. PMNJ1603-4904 seems to be either a highly peculiar BL Lac object or a misaligned jet source. In the latter case, the intriguing VLBI structure opens room for a possible classification of PMNJ1603-4904 as a gamma-ray bright CSO.

Multiwavelength↗

NASA NTRS2018

VLTI-GRAVITY Measurements of Cool Evolved Stars I. Variable Photosphere and Extended Atmosphere of the Mira Star R Peg

Context. Dynamic model atmospheres of Mira stars predict variabilities in the photospheric radius and in atmospheric molecular layers which are not yet strongly constrained by observations. Aim. Here we measure the variability of the oxygen-rich Mira star R Peg in near-continuum and molecular bands. Methods. We used near-infrared K-band spectro-interferometry with a spectral resolution of about 4000 obtained at four epochs between post-maximum and minimum visual phases employing the newly available GRAVITY beam combiner at the Very Large Telescope Interferometer (VLTI). Results. Our observations show a continuum radius that is anti-correlated with the visual lightcurve. Uniform disc (UD) angular diameters at a near-continuum wavelength of 2.24 m are steadily increasing with values of 8.7 0.1 mas, 9.4 0.1 mas, 9.8 0.1 mas, and 9.9 0.1 mas at visual phases of 0.15, 0.36, 0.45, 0.53, respectively. UD diameters at a bandpass around 2.05 m, dominated by water vapour, follow the near-continuum variability at larger UD diameters between 10.7 mas and 11.7 mas. UD diameters at the CO 2-0 bandhead, instead, are correlated with the visual lightcurve and anti-correlated with the near-continuum UD diameters, with values between 12.3 mas and 11.7 mas. Conclusions. The observed anti-correlation between continuum radius and visual lightcurve is consistent with an earlier study of the oxygen-rich Mira S Lac, and with recent 1D CODEX dynamic model atmosphere predictions. The amplitude of the variation is comparable to the earlier observations of S Lac, and smaller than predicted by CODEX models. The wavelength-dependent visibility variations at our epochs can be reproduced by a set of CODEX models at model phases between 0.3 and 0.6. The anti-correlation of water vapour and CO contributions at our epochs suggests that these molecules undergo different processes in the extended atmosphere along the stellar cycle. The newly available GRAVITY instrument is suited to conducting longer time series observations, which are needed to provide strong constraints on the model-predicted intra- and inter-cycle variability.

photospheric radius↗

NASA NTRS2020

The Fourth Catalog of Active Galactic Nuclei Detected by the Fermi Large Area Telescope

The fourth catalog of active galactic nuclei (AGNs) detected by the Fermi Gamma-ray Space Telescope Large Area Telescope (4LAC) between 2008 August 4 and 2016 August 2 contains $2863$ objects located at high Galactic latitudes (| b| > 10^9). It includes 85% more sources than the previous 3LAC catalog based on 4 yr of data. AGNs represent at least 79% of the high-latitude sources in the fourth Fermi-Large Area Telescope Source Catalog (4FGL), which covers the energy range from 50 MeV to 1 TeV. In addition, 344 gamma-ray AGNs are found at low Galactic latitudes. Most of the 4LAC AGNs are blazars (98%), while the remainder are other types of AGNs. The blazar population consists of 24% Flat Spectrum Radio Quasars (FSRQs), 38% BL Lac-type objects, and 38% blazar candidates of unknown types (BCUs). On average, FSRQs display softer spectra and stronger variability in the gamma-ray band than BL Lacs do, confirming previous findings. All AGNs detected by ground-based atmospheric Cerenkov telescopes are also found in the 4LAC.

M. Ajello↗

NASA NTRS2020

The Fourth Catalog of Active Galactic Nuclei Detected by the Fermi Large Area Telescope

The fourth catalog of active galactic nuclei (AGNs) detected by the Fermi Gamma-ray Space Telescope Large Area Telescope (4LAC) between 2008 August 4 and 2016 August 2 contains $2863$ objects located at high Galactic latitudes (|b|>10°). It includes 85% more sources than the previous 3LAC catalog based on 4 yr of data. AGNs represent at least 79% of the high-latitude sources in the fourth Fermi-Large Area Telescope Source Catalog (4FGL), which covers the energy range from 50 MeV to 1 TeV. In addition, 344 gamma-ray AGNs are found at low Galactic latitudes. Most of the 4LAC AGNs are blazars (98%), while the remainder are other types of AGNs. The blazar population consists of 24% Flat Spectrum Radio Quasars (FSRQs), 38% BL Lac-type objects, and 38% blazar candidates of unknown types (BCUs). On average, FSRQs display softer spectra and stronger variability in the gamma-ray band than BL Lacs do, confirming previous findings. All AGNs detected by ground-based atmospheric Cerenkov telescopes are also found in the 4LAC.

M. Ajello↗

NASA NTRS2021

Simultaneous Multiwavelength Flare Observations of EV Lacertae

We present the first results of our ongoing project conducting simultaneous multiwavelength observations of flares on nearby active M dwarfs. We acquired data of the nearby dM3.5e star EV Lac using five different observatories: NASA's Transiting Exoplanet Survey Satellite (TESS), NASA's Neil Gehrels Swift Observatory (Swift), NASA's Neutron Interior Composition Explorer (NICER), the University of Hawaii 2.2-meter telescope (UH88), and the Las Cumbres Observatory Global Telescope (LCOGT) Network. During the ∼25 days of TESS observations, we acquired three simultaneous UV/X-ray observations using Swift that total ∼18 ks, 21 simultaneous epochs totaling ∼98 ks of X-ray data using NICER, one observation (∼3 hr) with UH88, and one observation (∼3 hr) with LCOGT. We identified 56 flares in the TESS light curve with estimated energies in the range log E(T) (erg) = (30.5–33.2), nine flares in the Swift UVM2 light curve with estimated energies in the range log E(UV) (erg) = (29.3–31.1), 14 flares in the NICER light curve with estimated minimum energies in the range log E(N) (erg) = (30.5–32.3), and 1 flare in the LCOGT light curve with log E(L) (erg) = 31.6. We find that the flare frequency distributions (FFDs) of TESS and NICER flares have comparable slopes, β(T) = −0.67 ± 0.09 and β(N) = − 0.65 ± 0.19, and the FFD of UVOT flares has a shallower slope (β(U) = −0.38 ± 0.13). Furthermore, we do not find conclusive evidence for either the first ionization potential (FIP) or the inverse FIP effect during coronal flares on EV Lac.

Rishi R. Paudel↗

DOE OSTI2023

Reconstruction of dynamic scenes based on differences between collected view and synthesized view

A system for generating a 4D representation of a scene in motion given a sinogram collected from the scene while in motion. The system generates, based on scene parameters, an initial 3D representation of the scene indicating linear attenuation coefficients (LACs) of voxels of the scene. The system generates, based on motion parameters, a 4D motion field indicating motion of the scene. The system generates, based on the initial 3D representation and the 4D motion field, a 4D representation of the scene that is a sequence of 3D representations having LACs. The system generates a synthesized sinogram of the scene from the generated 4D representation. The system adjusts the scene parameters and the motion parameters based on differences between the collected sinogram and the synthesized sinogram. The processing is repeated until the differences satisfy a termination criterion.

Kim, Hyojin↗

DOE OSTI2023

The Global Climate Action Partnership

The Global Climate Action Partnership (GCAP; formerly the Low Emission Development Strategies Global Partnership) is a worldwide network that accelerates ambitious climate goals and advances implementation in Africa, Asia, and Latin America and the Caribbean (LAC). Our strategic approach emphasizes country-driven implementation actions to achieve resilient, just, and inclusive low-emission and net-zero economies. As an incubator for scalable knowledge and solutions, GCAP is leading the way to equitable, climate-resilient, low-emission development.

African Climate Action Partnership↗

DOE OSTI2021

A New Sample of Gamma-Ray Emitting Jetted Active Galactic Nuclei—Preliminary Results

We are compiling a new list of gamma-ray jetted active galactic nuclei (AGN), starting from the fourth catalog of point sources of the Fermi Large Area Telescope (LAT). Our aim is to prepare a list of jetted AGN with known redshifts and classifications to be used to calibrate jet power. We searched in the available literature for all the published optical spectra and multiwavelength studies useful to characterize the sources. We found new, missed, or even forgotten information leading to a substantial change in the redshift values and classification of many sources. We present here the preliminary results of this analysis and some statistics based on the gamma-ray sources with right ascension within the interval 0 h --12 h (J2000). Although flat-spectrum radio quasars and BL Lac objects are still the dominant populations, there is a significant increase in the number of other objects, such as misaligned AGN, narrow-line Seyfert 1 galaxies, and Seyfert galaxies. We also introduced two new classes of objects: changing-look AGN and ambiguous sources. About one third of the sources remain unclassified.

79 ASTRONOMY AND ASTROPHYSICS↗

NASA NTRS2022

The X-Ray Polarization View of Mrk 421 in an Average Flux State as Observed By the Imaging X-Ray Polarimetry Explorer

Particle acceleration mechanisms in supermassive black hole jets, such as shock acceleration, magnetic reconnection, and turbulence, are expected to have observable signatures in the multiwavelength polarization properties of blazars. The recent launch of the Imaging X-Ray Polarimetry Explorer (IXPE) enables us, for the first time, to use polarization in the X-ray band (2–8 keV) to probe the properties of the jet synchrotron emission in high-synchrotron-peaked BL Lac objects (HSPs). We report the discovery of X-ray linear polarization (degree Π x = 15% ± 2% and electric vector position angle ψ x = 35° ± 4°) from the jet of the HSP Mrk 421 in an average X-ray flux state. At the same time, the degree of polarization at optical, infrared, and millimeter wavelengths was found to be lower by at least a factor of 3. During the IXPE pointing, the X-ray flux of the source increased by a factor of 2.2, while the polarization behavior was consistent with no variability. The higher level of Π x compared to longer wavelengths, and the absence of significant polarization variability, suggest a shock is the most likely X-ray emission site in the jet of Mrk 421 during the observation. The multiwavelength polarization properties are consistent with an energy-stratified electron population, where the particles emitting at longer wavelengths are located farther from the acceleration site, where they experience a more disordered magnetic field.

acceleration of particles↗

DOE OSTI2021

Enhancing transcription in Escherichia coli and Pseudomonas putida using bacteriophage lambda anti-terminator protein Q

Functional characterization of metagenomic DNA often involves expressing heterologous DNA in genetically tractable microorganisms such as Escherichia coli . Functional expression of heterologous genes can suffer from limitations due to the lack of recognition of foreign promoters or presence of intrinsic terminators on foreign DNA between a vector-based promoter and the transcription start site. Anti-terminator proteins are a possible solution to overcome this limitation. When bacteriophage lambda infects E. coli , it relies on the host transcription machinery to transcribe and express phage DNA. Lambda anti-terminator protein Q (λQ) regulates the expression of late-genes of phage lambda. E. coli RNA polymerase recognizes the P R ' promoter on the lambda genome and forms a complex with λQ, to overcome the terminator t R '. In this study we show the use of λQ to efficiently transcribe a capsular polysaccharide cluster, cps3 , from Lactobacillus plantarum containing intrinsic terminators in Escherichia coli . In addition, we expand the use of anti-terminator λQ in Pseudomonas putida . The results show ~ fivefold higher expression of a fluorescent reporter locate ~ 12.5kbp downstream from the promoter, when the transcription is driven by P R ' promoter in presence of λQ compared to a lac promoter. These results suggest that λQ could be used in metabolic engineering to enhance expression of heterologous DNA.

59 BASIC BIOLOGICAL SCIENCES↗

DOE OSTI2025

Discovery, characterization, and application of chromosomal integration sites in the hyperthermophilic archaeon Sulfolobus islandicus

Sulfolobus islandicus , an emerging archaeal model organism, offers unique advantages for metabolic engineering and synthetic biology applications owing to its ability to thrive in extreme environments. Although several genetic tools have been established for this organism, the lack of well-characterized chromosomal integration sites has limited its potential as a cellular factory. Here, in this work, we systematically identified and characterized 13 artificial CRISPR RNAs targeting eight integration sites in S. islandicus using the CRISPR-COPIES pipeline and a multi-omics-informed computational workflow. We leveraged the endogenous CRISPR-Cas system to integrate the reporter gene lacS and validated heterologous expression through a β-galactosidase assay, revealing significant positional effects. As a proof of concept, we utilized these sites to genetically manipulate lipid ether composition by overexpressing glycerol dibiphytanyl glycerol tetraether (GDGT) ring synthase B (GrsB). This study expands the genetic toolbox for S. islandicus and advances its potential as a robust platform for archaeal synthetic biology and industrial biotechnology.

59 BASIC BIOLOGICAL SCIENCES↗

DOE OSTI2024

Optimized CRISPR Interference System for Investigating Pseudomonas alloputida Genes Involved in Rhizosphere Microbiome Assembly

Pseudomonas alloputida KT2440 (formerly P. putida) has become both a well-known chassis organism for synthetic biology and a model organism for rhizosphere colonization. Here, we describe a CRISPR interference (CRISPRi) system in KT2440 for exploring microbe–microbe interactions in the rhizosphere and for use in industrial systems. Our CRISPRi system features three different promoter systems (XylS/P m , LacI/P lac , and AraC/P BAD ) and a dCas9 codon-optimized for Pseudomonads, all located on a mini-Tn7-based transposon that inserts into a neutral site in the genome. It also includes a suite of pSEVA-derived sgRNA expression vectors, where the expression is driven by synthetic promoters varying in strength. We compare the three promoter systems in terms of how well they can precisely modulate gene expression, and we discuss the impact of environmental factors, such as media choice, on the success of CRISPRi. We demonstrate that CRISPRi is functional in bacteria colonizing the rhizosphere, with repression of essential genes leading to a 10–100-fold reduction in P. alloputida cells per root. Finally, we show that CRISPRi can be used to modulate microbe–microbe interactions. When the gene pvdH is repressed and P. alloputida is unable to produce pyoverdine, it loses its ability to inhibit other microbes in vitro. Furthermore, our design is amendable for future CRISPRi-seq studies and in multispecies microbial communities, with the different promoter systems providing a means to control the level of gene expression in many different environments.

Bacteria↗

DOE OSTI2020

Simultaneous observations of the blazar PKS 2155-304 from ultra-violet to TeV energies

Here we report the results of the first ever contemporaneous multi-wavelength observation campaign on the BL Lac object PKS 2155−304 involving Swift , NuSTAR , Fermi -LAT, and H.E.S.S. The use of these instruments allows us to cover a broad energy range, which is important for disentangling the different radiative mechanisms. The source, observed from June 2013 to October 2013, was found in a low flux state with respect to previous observations but exhibited highly significant flux variability in the X-rays. The high-energy end of the synchrotron spectrum can be traced up to 40 keV without significant contamination by high-energy emission. A one-zone synchrotron self-Compton model was used to reproduce the broadband flux of the source for all the observations presented here but failed for previous observations made in April 2013. A lepto-hadronic solution was then explored to explain these earlier observational results.

79 ASTRONOMY AND ASTROPHYSICS↗

DOE OSTI2023

Long-term multi-wavelength study of 1ES 0647+250

The BL Lac object 1ES 0647+250 is one of the few distant γ-ray emitting blazars detected at very high energies (VHEs; &100 GeV) during a non-flaring state. It was detected with the MAGIC telescopes during a period of low activity in the years 2009-2011 as well as during three flaring activities in the years 2014, 2019, and 2020, with the highest VHE flux in the last epoch. An extensive multi-instrument data set was collected as part of several coordinated observing campaigns over these years. We aim to characterise the long-term multi-band flux variability of 1ES 0647+250, as well as its broadband spectral energy distribution (SED) during four distinct activity states selected in four different epochs, in order to constrain the physical parameters of the blazar emission region under certain assumptions. We evaluated the variability and correlation of the emission in the different energy bands with the fractional variability and the Z transformed discrete correlation function, as well as its spectral evolution in X-rays and γ rays. Owing to the controversy in the redshift measurements of 1ES 0647+250 reported in the literature, we also estimated its distance in an indirect manner through a comparison of the GeV and TeV spectra from simultaneous observations with Fermi-LAT and MAGIC during the strongest flaring activity detected to date. Moreover, we interpret the SEDs from the four distinct activity states within the framework of one-component and two-component leptonic models, proposing specific scenarios that are able to reproduce the available multi-instrument data. We find significant long-term variability, especially in X-rays and VHE γ rays. Furthermore, significant (3-4σ) correlations were found between the radio, optical, and high-energy (HE) γ-ray fluxes, with the radio emission delayed by about ~400 days with respect to the optical and γ-ray bands. The spectral analysis reveals a harder-when-brighter trend during the non-flaring state in the X-ray domain. However, no clear patterns were observed for either the enhanced states or the HE (30 MeV < E < 100 GeV) and VHE γ-ray emission of the source. The indirect estimation of the redshift yielded a value of z = 0.45 ± 0.05, which is compatible with some of the values reported in the literature. The SEDs related to the low-activity state and the three flaring states of 1ES 0647+250 can be described reasonably well with the both one-component and two-component leptonic scenarios. However, the long-term correlations indicate the need for an additional radio-producing region located about 3.6 pc downstream from the gamma-ray producing region.

79 ASTRONOMY AND ASTROPHYSICS↗