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At least 253 records · Page 14

A multi-level solution algorithm for steady-state Markov chains

A new iterative algorithm, the multi-level algorithm, for the numerical solution of steady state Markov chains is presented. The method utilizes a set of recursively coarsened representations of the original system to achieve accelerated convergence. It is motivated by multigrid methods, which are widely used for fast solution of partial differential equations. Initial results of numerical experiments are reported, showing significant reductions in computation time, often an order of magnitude or more, relative to the Gauss-Seidel and optimal SOR algorithms for a variety of test problems. The multi-level method is compared and contrasted with the iterative aggregation-disaggregation algorithm of Takahashi.

Horton, Graham↗

Ionospheric corrections to precise time transfer using GPS

The free electrons in the earth's ionosphere can retard the time of reception of GPS signals received at a ground station, compared to their time in free space, by many tens of nanoseconds, thus limiting the accuracy of time transfer by GPS. The amount of the ionospheric time delay is proportional to the total number of electrons encountered by the wave on its path from each GPS satellite to a receiver. This integrated number of electrons is called Total Electron Content, or TEC. Dual frequency GPS receivers designed by Allen Osborne Associates, Inc. (AOA) directly measure both the ionospheric differential group delay and the differential carrier phase advance for the two GPS frequencies and derive from this the TEC between the receiver and each GPS satellite in track. The group delay information is mainly used to provide an absolute calibration to the relative differential carrier phase, which is an extremely precise measure of relative TEC. The AOA Mini-Rogue ICS-4Z and the AOA TurboRogue ICS-4000Z receivers normally operate using the GPS P code, when available, and switch to cross-correlation signal processing when the GPS satellites are in the Anti-Spoofing (A-S) mode and the P code is encrypted. An AOA ICS-Z receiver has been operated continuously for over a year at Hanscom AFB, MA to determine the statistics of the variability of the TEC parameter using signals from up to four different directions simultaneously. The 4-channel ICS-4Z and the 8-channel ICS-4000Z, have proven capabilities to make precise, well calibrated, measurements of the ionosphere in several directions simultaneously. In addition to providing ionospheric corrections for precise time transfer via satellite, this dual frequency design allows full code and automatic codeless operation of both the differential group delay and differential carrier phase for numerous ionospheric experiments being conducted. Statistical results of the data collected from the ICS-4Z during the initial year of ionospheric time delay in the northeastern U.S., and initial results with the ICS-4000Z, will be presented.

Snow, Robert W.↗

Kinetics of phase transformation in glass forming systems

The objectives of this research were to (1) develop computer models for realistic simulations of nucleation and crystal growth in glasses, which would also have the flexibility to accomodate the different variables related to sample characteristics and experimental conditions, and (2) design and perform nucleation and crystallization experiments using calorimetric measurements, such as differential scanning calorimetry (DSC) and differential thermal analysis (DTA) to verify these models. The variables related to sample characteristics mentioned in (1) above include size of the glass particles, nucleating agents, and the relative concentration of the surface and internal nuclei. A change in any of these variables changes the mode of the transformation (crystallization) kinetics. A variation in experimental conditions includes isothermal and nonisothermal DSC/DTA measurements. This research would lead to develop improved, more realistic methods for analysis of the DSC/DTA peak profiles to determine the kinetic parameters for nucleation and crystal growth as well as to assess the relative merits and demerits of the thermoanalytical models presently used to study the phase transformation in glasses.

Ray, Chandra S.↗

Early results from the TOPEX/POSEIDON GPS precise orbit determination demonstration

TOPEX/POSEIDON, a US/French oceanographic mission launched in August 1992, is the first earth satellite to carry a multi-channel, dual frequency Global Positioning System (GPS) receiver capable of making high precision P-code pseudorange and carrier phase measurements. The receiver was placed on TOPEX/POSEIDON as an experiment to demonstrate the potential of differential GPS tracking for subdecimeter orbit determination. In addition to the receiver, TOPEX/POSEIDON carries two flight-proven tracking systems to provide the operational precise orbit determination needed to meet the mission scientific requirements. These include a French-built one-way Doppler system known as DORIS (Doppler Orbitography and Radiopositioning Integrated by Satellite) and a circular ring of laser retroreflectors. Here we evaluate the quality of the GPS-determined orbits by examining post-fit residuals, orbit comparisons with DORIS, and orbit repeatability on overlapping data arcs. Overlapping data arcs with 6 hrs of common data out of a 30-hr arc have an average root-mean-square (RMS) altitude difference of 3.0 cm for 9 arcs. The average RMS altitude difference about the mean with a DORIS orbit was 5.7 cm.

Bertiger, Willy↗

Comparisons of the Maxwell and CLL gas/surface interaction models using DSMC

The behavior of two different models of gas-surface interactions is studied using the Direct Simulation Monte Carlo (DSMC) method. The DSMC calculations examine differences in predictions of aerodynamic forces and heat transfer between the Maxwell and the Cercignani-Lampis-Lord (CLL) models for flat plate configurations at freestream conditions corresponding to a 140 km orbit around Venus. The size of the flat plate represents one of the solar panels on the Magellan spacecraft, and the freestream conditions correspond to those experienced during aerobraking maneuvers. Results are presented for both a single flat plate and a two-plate configuration as a function of angle of attack and gas-surface accommodation coefficients. The two-plate system is not representative of the Magellan geometry but is studied to explore possible experiments that might be used to differentiate between the two gas-surface interaction models. The Maxwell and CLL models produce qualitatively similar results for the aerodynamic forces and heat transfer on a single flat plate. However, the flow fields produced with the two models are qualitatively different for both the single-plate and two-plate calculations. These differences in the flowfield lead to predictions of the angle of attack for maximum heat transfer in a two plate configuration that are distinctly different for the two gas-surface interactions models.

Hedahl, Marc O.↗

Feedback Control of Plasma Instabilities with Charged Particle Beams and Study of Plasma Turbulence

A new non-perturbing technique for feedback control of plasma instabilities has been developed in the Columbia Linear Machine (CLM). The feedback control scheme involves the injection of a feedback modulated ion beam as a remote suppressor. The ion beam was obtained from a compact ion beam source which was developed for this purpose. A Langmuir probe was used as the feedback sensor. The feedback controller consisted of a phase-shifter and amplifiers. This technique was demonstrated by stabilizing various plasma instabilities to the background noise level, like the trapped particle instability, the ExB instability and the ion-temperature-gradient (ITG) driven instability. An important feature of this scheme is that the injected ion beam is non-perturbing to the plasma equilibrium parameters. The robustness of this feedback stabilization scheme was also investigated. The principal result is that the scheme is fairly robust, tolerating about 100% variation about the nominal parameter values. Next, this scheme is extended to the unsolved general problem of controlling multimode plasma instabilities simultaneously with a single sensor-suppressor pair. A single sensor-suppressor pair of feedback probes is desirable to reduce the perturbation caused by the probes. Two plasma instabilities the ExB and the ITG modes, were simultaneously stabilized. A simple 'state' feedback type method was used where more state information was generated from the single sensor Langmuir probe by appropriate signal processing, in this case, by differentiation. This proof-of-principle experiment demonstrated for the first time that by designing a more sophisticated electronic feedback controller, many plasma instabilities may be simultaneously controlled. Simple theoretical models showed generally good agreement with the feedback experimental results. On a parallel research front, a better understanding of the saturated state of a plasma instability was sought partly with the help of feedback. A plasma instability is usually observed in its saturated state and appears as a single feature in the frequency spectrum with a single azimuthal and parallel wavenumbers. The physics of the non-zero spectral width was investigated in detail because the finite spectral width can cause "turbulent" transport. One aspect of the "turbulence" was investigated by obtaining the scaling of the linear growth rate of the instabilities with the fluctuation levels. The linear growth rates were measured with the established gated feedback technique. The research showed that the ExB instability evolves into a quasi-coherent state when the fluctuation level is high. The coherent aspects were studied with a bispectral analysis. Moreover, the single spectral feature was discovered to be actually composed of a few radial harmonics. The radial harmonics play a role in the nonlinear saturation of the instability via three-wave coupling.

Tham, Philip Kin-Wah↗

DGPS with NASA's ACTS

The U.S. Army Topographic Engineering Center, concerned about frequency license problems inherent with operating data-ready radios to support differential GPS (DGPS), learned of an experiment program the National Aeronautics and Space Administration (NASA) was sponsoring with its prototype digital satellite - the Advanced Communications Technology Satellite (ACTS). ACTS was designed to evaluate many new, untested technologies for eventual transfer to U.S. telecommunications and related industries. The new technologies promised greater data transmission rates and significantly reduced transmission latency. recognizing these benefits, the Topographic Engineering Center designed a DGPS experiment using an ACTS datalink with baselines of 200 kilometers and longer to learn what positional accuracy was possible with leading-edge satellite telecommunications technology.

Dendy, Roger↗

Comparisons of the Maxwell and CLL Gas/Surface Interaction Models Using DSMC

Two contrasting models of gas-surface interactions are studied using the Direct Simulation Monte Carlo (DSMC) method. The DSMC calculations examine differences in predictions of aerodynamic forces and heat transfer between the Maxwell and Cercignani-Lampis-Lord (CLL) models for flat plate configurations at freestream conditions corresponding to a 140 km orbit around Venus. The size of the flat plate is that of one of the solar panels on the Magellan spacecraft, and the freestream conditions are one of those experienced during aerobraking maneuvers. Results are presented for both a single flat plate and a two-plate configuration as a function of angle of attack and gas-surface accommodation coefficients. The two plate system is not representative of the Magellan geometry, but is studied to explore possible experiments that might be used to differentiate between the two gas surface interaction models.

Hedahl, Marc O.↗

Chemical and Thermal Analysis

During the past six months we have conducted significant research in several domains in order to clarify and understanding the aging and chemical failure mechanism of thermoplastics (PVDF or Tefzel) for pipes. We organized numerous analytical studies with methods including Fourier Transform Infrared Spectroscopy, Dynamic Mechanical Analysis, Differential Scanning Calorimetry, and Stress Relaxation experiments. In addition we have reanalyzed previous thermogravimetric data concerning the rate of deplasticization of Coflon pipe. We investigated a number of aged samples of both Tefzel and Coflon that were forwarded from MERL. We conducted stress relaxation experiments of Coflon pipe at several temperatures and determined an activation energy. We also examined the dynamic mechanical response PVDF during deplasticization and during methanol plasticization. We performed numerous DSC analyses to research the changing crystalline morphology. We have noted significant changes in crystallinity upon aging for both PVDF and Tefzel. Little variation in elemental composition was noted for many of the aged Coflon and Tefzel samples tested.

Bulluck, J. W.↗

Retrieval and Analysis of Stratospheric NO2 from GOME

In this paper we describe the retrieval of stratospheric NO2 from the GOME (Global Ozone Monitoring Experiment) spectrometer. For this retrieval the Differential Optical Absorption Spectroscopy (DOAS) is used and we describe the influence of the instrument s characteristics on this measurement technique. This analysis led to an improved version of the DOAS algorithm resulting in results with lower systematic errors. Subsequently these results were used to separate the tropospheric and stratospheric fraction of the measured NO;! in the atmosphere. This paper is focusing on the annual variations of the stratospheric distribution of nitrogen oxides. For this examination the satellite data from beginning of 1996 to the end of 2001 was used and has been visualized in a plot zonal means versus time of the year, a visualization which proved to be very useful for Ozone. Additionally the so called "Noxon Cliff", a drop of NO2 column densities Noxon measured in 1975-77 while traveling northwards towards the pole in Canada, is shown. Also its southern equivalent could be discovered in the GOME data.

Wenig, M.↗

The Search for Lunar Mantle Rocks Exposed on the Surface of the Moon

The lunar surface is ancient and well-preserved, recording Solar System history and planetary evolution processes. Ancient basin-scale impacts excavated lunar mantle rocks, which are still expected to be present on the surface. Sampling these rocks would provide insight into fundamental planetary processes, including differentiation and magmatic evolution. There is contention among lunar scientists as to what lithologies make up the upper lunar mantle, and where they may have been exposed on the surface. We review dynamical models of lunar differentiation in the context of recent experiments and spacecraft data, assessing candidate lithologies, their distribution, and implications for lunar evolution.

Geochemistry↗

Differential wall growth in gravistimulated corn roots: Its timing and regulation

The experiments designed to document cell-wall level changes which occur as a result of their gravistimulation are described. The goal of this research is to elucidate the mechanism and time frame of differential growth following a controlled gravistimulation. To achieve this, rates of wall deposition will be determined by following the incorporation of radioactive monosaccharides into the wall. Complementing this experiment will be a freeze-etch study directed at revealing the spatial arrangment of both newly-deposited microfibrils and microfibrils that were present in the growing root prior to stimulation. The second phase of the proposed research will examine the roles ethylene and Ca(2+) have in the modulation of differential wall changes during gravitropism. Ethylene and Ca(2+) have both been implicated as regulators of the gravitropic response in roots and they have also been implicated as regulators of the gravitropic response in roots and they have also been reported to exert some control on the orientation of microfibrils. Both of these agents will be manipulated in such a way as to reveal whether they have a direct influence on cell wall deposition and microfibrillar alignment during the geotropic response.

Serlin, B. S.↗

Development of a deep learning based automated data analysis for step-filter x-ray spectrometers in support of high-repetition rate short-pulse laser-driven acceleration experiments

We present a deep learning based framework for real-time analysis of a differential filter based x-ray spectrometer that is common on short-pulse laser experiments. The analysis framework was trained with a large repository of synthetic data to retrieve key experimental metrics, such as slope temperature. With traditional analysis methods, these quantities would have to be extracted from data using a time-intensive and manual analysis. Furthermore, this framework was developed for a specific diagnostic, but may be applicable to a wide variety of diagnostics common to laser experiments and thus will be especially crucial to the development of high-repetition rate (HRR) diagnostics for HRR laser systems that are coming online.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗

On differentiable local bounds preserving stabilization for Euler equations

This work presents the design of nonlinear stabilization techniques for the finite element discretization of Euler equations in both steady and transient form. Implicit time integration is used in the case of the transient form. A differentiable local bounds preserving method has been developed, which combines a Rusanov artificial diffusion operator and a differentiable shock detector. Nonlinear stabilization schemes are usually stiff and highly nonlinear. This issue is mitigated by the differentiability properties of the proposed method. Moreover, in order to further improve the nonlinear convergence, we also propose a continuation method for a subset of the stabilization parameters. The resulting method has been successfully applied to steady and transient problems with complex shock patterns. Numerical experiments show that it is able to provide sharp and well resolved shocks. Furthermore, the importance of the differentiability is assessed by comparing the new scheme with its non-differentiable counterpart. Numerical experiments suggest that, for up to moderate nonlinear tolerances, the method exhibits improved robustness and nonlinear convergence behavior for steady problems. Additionally, in the case of transient problem, we also observe a reduction in the computational cost.

42 ENGINEERING↗

Phase partitioning experiment (8-IML-1)

Phase partitioning is a method of separating biological cells and macromolecules via their differential distribution in two phase aqueous polymer solutions. The ultimate goal of the experiment is to test the hypothesis that the efficiency of separation of closely related cell types, by partitioning in immiscible aqueous phases, will be enhanced in the non-convective environment provided by space. Before a cell separation experiment can be performed, the demixing of immiscible aqueous polymer solutions must be understood and controlled in order to optimize the experimental conditions for a cell separation experiment in the future. The present Phase Partitioning Experiment (PPE) is the third in a series, the first two flew on STS 51-D in Apr. 1985 and STS 26 in Oct. 1988. In those experiments the immiscible aqueous phases demixed spontaneously at different rates, the final disposition being one in which the phase which wetted the container wall surrounded the second phase which formed an 'egg yolk' in the center of the chamber.

Brooks, Donald E.↗

Studies on penetration of antibiotic in bacterial cells in space conditions (7-IML-1)

The Cytos 2 experiment was performed aboard Salyut 7 in order to test the antibiotic sensitivity of bacteria cultivated in vitro in space. An increase of the Minimal Inhibitory Concentration (MIC) in the inflight cultures (i.e., an increase of the antibiotic resistance) was observed. Complementary studies of the ultrastructure showed a thickening of the cell envelope. In order to confirm the results of the Cytos 2 experiment, we performed the ANTIBIO experiment during the D1 mission to try to differentiate, by means of the 1 g centrifuge in the Biorack, between the biological effects of cosmic rays and those caused by microgravity conditions. The originality of this experiment was in the fact that it was designed to test the antibiotic sensitivity of bacteria cultivated in vitro during the orbital phase of the flight. The results show an increase in resistance to Colistin in in-flight bacteria. The MIC is practically double in the in-flight cultures. A cell count of living bacteria in the cultures containing the different Colistin concentrations showed a significant difference between the cultures developed during space flight and the ground based cultures. The comparison between the 1 g and 0 g in-flight cultures show similar behavior for the two sets. Nevertheless, a small difference between the two sets of ground based control cultures was noted. The cultures developed on the ground centrifuge (1.4 g) present a slight decrease in comparison with the cultures developed in the static rack (1 g). In order to approach the mechanisms of the increase of antibiotic resistance on bacteria cultivated in vitro in space, we have proposed the study on penetration of antibiotics in bacterial cells in space conditions. This experiment was selected for the International Microgravity Laboratory 1 (IML-1) mission.

Tixador, R.↗

A Pipeline for Assessing the Quality of Rna-Seq Datasets in GeneLab

Transcriptome profiling by RNA sequencing (RNA-seq) is a powerful approach to identify gene expression changes in organisms exposed to unique environments such as spaceflight. One of the challenges of evaluating RNA-seq data both within and across different space-relevant studies is the ability to control for technical differences, including the use of different library preparation kits, sequencing platforms, RNA yield, and person-to-person variation. To help address this issue, the National Institute of Standards and Technology (NIST, nist.gov) initiated a consortium, at the request of industry and academia, to develop a set of controls for gene expression measurements. The result was a set of 92 unlabeled, polyadenylated transcripts that range from 250 – 2,000 nucleotides in length to mimic natural eukaryotic mRNAs. These External RNA Controls Consortium (ERCC) genes can be used in any RNA-seq experiment, by adding known concentrations of the ERCC genes to samples after RNA extraction, to offer a standard measurement for data comparison. At NASA GeneLab, we employ these controls as part of our standard operating procedures for every in-house RNA-seq study to assess the limit of detection, dynamic range, and power of differential expression analysis both within and across experiments. Here we will discuss the use, benefits, and limitations of ERCC genes and other types of controls, such as universal RNA references, to generate quality control information for RNA-seq studies conducted at GeneLab.

GeneLab↗

Osteoblastic phenotype of rat marrow stromal cells cultured in the presence of dexamethasone, beta-glycerolphosphate, and L-ascorbic acid

We investigated the effects of the time course of addition of osteogenic supplements dexamethasone, beta-glycerolphosphate, and L-ascorbic acid to rat marrow stromal cells, and the exposure time on the proliferation and differentiation of the cells. It was the goal of these experiments to determine the time point for supplement addition to optimize marrow stromal cell proliferation and osteoblastic differentiation. To determine this, two studies were performed; one study was based on the age of the cells from harvest, and the other study was based on the duration of exposure to supplemented medium. Cells were seen to proliferate rapidly at early time points in the presence and absence of osteogenic supplements as determined by 3H-thymidine incorporation into the DNA of replicating cells. These results were supported by cell counts ascertained through total DNA analysis. Alkaline phosphatase (ALP) activity and osteocalcin production at 21 days were highest for both experimental designs when the cells were exposed to supplemented medium immediately upon harvest. The ALP levels at 21 days were six times greater for cells maintained in supplements throughout than for control cells cultured in the absence of supplements for both studies, reaching an absolute value of 75 x 10(-7) micromole/min/cell. Osteocalcin production reached 20 x 10(-6) ng/cell at 21 days in both studies for cells maintained in supplemented medium throughout the study, whereas the control cells produced an insignificant amount of osteocalcin. These results suggest that the addition of osteogenic supplements to marrow-derived cells early in the culture period did not inhibit proliferation and greatly enhanced the osteoblastic phenotype of cells in a rat model.

Non-NASA Center↗